ISSAAS 2019 - erepo.unud.ac.id

ISSAAS 2019

18 – 20 OCTOBER 2019 | UPM, SERDANG, SELANGOR, MALAYSIA

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BOOK OF ABSTRACTS

ISSAAS 2019 International Congress & General Meeting

“RESHAPING AGRICULTURE FOR SUSTAINABLE DEVELOPMENT”

Editors:

Abdul Shukor Juraimi

Rita Muhamad Awang

Uma Rani Sinniah

Juwaidah Sharifuddin

Erneeza Mohd Hata

Anis Syahirah Mokhtar

Nurul Nadia Ramli

ISSAAS 2019


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TABLE OF CONTENT

Plenary Abstracts

o Indonesia iii o Japan iv o Malaysia v o Philippines vi o Thailand vii o Vietnam viii

Program at-a-Glance x List of Presentations 1- 15 Oral Presentations 16 - 158 Poster Presentations 159 - 211

ISSAAS 2019


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PROGRAM AT-A-GLANCE

18 OCTOBER 2019 (FRIDAY) Time Auditorium Angsana Putra 1 Angsana Putra 2 Wacana Putra 2 Wacana Putra 3

14.30-16.00 Chairman: Dr. Nguyen Thi

Bich Thuy (Vietnam)

Chairman: Assoc. Prof. Dr. Nur

Azura Adam (Malaysia)

Chairman: Dr. Machito Mihara

(Japan)

Chairman: Dr. Nina Shimoguchi

(Japan)

Chairman: Prof. Maria Endo

Mahata (Indonesia)

Crop Science/Plant Science I

CS01-CS06

Entomology I ENTO01-ENTO05

Smart Farming/Digital Agriculture I

SFDA01-SFDA05

Agribusiness/ Agricultural Economics I

AEC01-AEC06

Animal Science and Production I AS01-AS06

16.00-16.15 COFFEE BREAK & POSTER SESSION 16.15-17.15 Chairman:

Prof. Dr. Sreeramanan Subramaniam

(Malaysia)

Crop Science/Plant Science II

CS07-CS10

Chairman: Prof. Dr. Dadang

(Indonesia)

Entomology II ENTO06-ENTO07

Agricultural Chemistry I AC01-AC02

Chairman: Dr. Ahmad Suhaizi

Mat Su (Malaysia)

Agro-Engineering I AEG01-AEG03

Chairman: Assoc. Prof. Mukhamad

Najib (Indonesia)

Agribusiness/ Agricultural Economics II

AEC07-AEC10

Chairman: Prof. Dr. Janjira Phavaphutanon

(Thailand)

Animal Science and Production II AS07-AS10

19 OCTOBER 2019 (SATURDAY) 8.30-10.00 Chairman:

Dr. Evalour Aspuria

(Philippines)

Chairman: Dr. Suwardi (Indonesia)

Soil Science I SS01-SS04

Forestry I FOR01

Chairman: Assoc. Prof. Dr. Khairulmazmi

Ahmad (Malaysia)

Plant Pathology I PATHO01- PATHO06

Chairman: Dr. Nguyen Thi Minh

Hien (Vietnam)

Agribusiness/ Agricultural Economics

III AEC11-AEC16

Chairman: Prof. Dr. Nuraini

(Indonesia)

Crop Science/Plant Science III CS11-CS15

Animal Science and Production III AS11-AS16

10.00-10.30 COFFEE BREAK & POSTER SESSION

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Time Auditorium Angsana Putra 1 Angsana Putra 2 Wacana Putra 2 Wacana Putra 3

10.30-11.30 Chairman: Dr. Sasitorn Nakthong (Thailand)

Plant Production

and Environmental Agriculture I

PPEA01-PPEA04

Chairman: Dr. Naroon Waramit

(Thailand)

Plant Production and Environmental Agriculture

II PPEA05-PPEA08

Chairman: Assoc. Prof. Dr.

Maria Lima Diongzon Pascial

(Philippines)

Plant Pathology II PATHO07- PATHO10

Chairman: Dr. Susanita Lumbo

(Philippines)

Agribusiness/ Agricultural Economics

IV AEC17-AEC20

Chairman: Dr. Nieta Colineras

Amit (Philippines)

Animal Science and

Production IV AS17-AS20

11.30-12.30 Chairman: Prof. Dr. Ghizan

Saleh (Malaysia)

Chairman: Prof. Dr. Itji Diana Daud

(Indonesia)

Chairman: Prof. Baharuddin

(Indonesia)

Chairman: Assoc. Prof. Dr. I. Wayan Budiasa

(Indonesia)

Chairman: Dr. Tee Tuan Poy

(Malaysia)

Plant Production

and Environmental Agriculture III

PPEA09-PPEA13

Plant Production and Environmental Agriculture

IV PPEA14-PPEA18

Plant Pathology III PATHO11- PATHO14

Agribusiness/

Agricultural Economics V AEC21-AEC24

Animal Science and Production V AS21-AS22

Aquatic Science I AQS01-AQS02

12.30-14.00 LUNCH BREAK & POSTER SESSION

14.00-15.30 Chairman: Dr. Susan

Calumpang (Philippines)

Chairman: Prof. Dr. Kenji

Yokoto (Japan)

Chairman: Prof. Tutik Kuswinanti (Indonesia)

Chairman: Dr. Nur Bahiah Mohamed Haris

(Malaysia)

Chairman: Assoc. Prof. Dr. Norida Mazlan

(Malaysia)

Plant Production

and Environmental Agriculture V

PPEA19-PPEA24

Plant Pathology V PATHO20-PATHO24

Plant Pathology IV PATHO15- PATHO19

Agricultural Extension I AEX01-AEX06

Agricultural Extension II

AEX07-AEX10

15.30-16.00 COFFEE BREAK 16.00-17.30 CLOSING CEREMONY

ISSAAS 2019


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ORAL AND POSTER PRESENTATIONS

LIST OF ORAL PRESENTATIONS

Agribusiness/Agricultural Economics

Session Info Paper ID

Presenter Title

Agribusiness/ Agricultural Economics I Oct. 18 (Friday) 14.30-16.00 Wacana Putra 2

AEC01 Nia Kurniawati Hidayat

Livelihood Resilience of Indonesian Palm Oil Smallholders: An Analysis Among Different Types of Smallholders with and without Certification

AEC02 Blas Enrique Hahn Libardi

Sesame Sesamum Indicum (L.) Production of Paraguay With Emphasis to Japanese Market Value Chain Analysis

AEC01-AEC06

AEC03 Melissa Alina Yusoff

Determining Ranchers’ Willingness-To-Pay (WTP) For New Dairy Goat Feeding Technology in Peninsular Malaysia, Malaysia

AEC04 Komang Wibuti Emriko

Domestic Behavior Indonesia

and on

Foreign Traditional

Consumers’

Medicines Purchasing (Jamu) in

AEC05 Susanita G. Lumbo

Valuing of Lowland Rice Culture and Its Relation to the Social Well-Being and Status of Upland Resources of Tau-Buid in Occidental Mindoro, Philippines

AEC06 I Wayan Budiasa Developing Agricultural Cooperative Model for Supporting Subak-Based Agribusiness Development Within the World Culture Heritage Site, Bali Indonesia

Agribusiness/ Agricultural Economics II Oct. 18 (Friday) 16.15-17.15 Wacana Putra 2

AEC07 Bounpathana Kanyavong

Improvement in Livelihood of Organic Vegetable Producer Group in Laos: Case Study from Thaxang Village, Vientiane

AEC08 Dipendra Dhakal Study on The Potentiality of Resilience of Rural People for Disaster Recovery. A Case Study from Nepal

AEC07-AEC10

AEC09 Dina Lianita Sari Food Waste in University Canteens: Does It Threat Food Security?

AEC10 Kaori Amano A Case Study of The Consumers Buying Behavior for Branded Tomato in Nara, Japan

Agribusiness/ Agricultural Economics III Oct. 19 (Saturday) 8.30-10.00 Wacana Putra 2

AEC11-AEC16

AEC11 Silvia Dewi Sagita Andik

Halal Food Purchase Intention Among Muslim Consumers in Indonesia: A Structural Equation Modeling With Partial Least Square Approach

AEC12 Normalyn T. Longay

Value Chain of Strawberry in La Trinidad, Benguet, Philippines

AEC13 Sayed Abdul Wahab Saeedi

Adoption of Industry 4.0 Technologies Among SMEs in Malaysian Dairy Manufacturing Industry: Are They Ready?

ISSAAS 2019


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Presenter Title

AEC14 Christine Joy B. Manalo

Fluctuations in Supply and Prices of White Potato and Tomato: Are the Triggers the Same?

AEC15 A Faroby Falatehan

Fertilizer Subsidy, Post-Harvest Technology and Rice Loss Nexus in Indonesia

AEC16 Chairani Putri Pratiwi

The Development Strategy of Indonesian Floriculture Business

Agribusiness/ Agricultural Economics IV Oct. 19 (Saturday) 10.30-11.30 Wacana Putra 2

AEC17-AEC20

AEC17 Hamidu Abubakar Kara

Socio-Economic Factors Influencing Rice Production in Kebbi State, Nigeria

AEC18 Rahmat Yanuar Differences in Indonesian Dairy Industry Performance: A Comparison Between Two Regions

AEC19 Jan Idel Emmanuel Castañeda

Utilization of A Crop Model for Decision Tools in Rice Production: The Case of Calapan, Oriental Mindoro, Philippines

AEC20 Alia Khalidah Ismail

Consumer’s Preferences of Goat Milk Based on

Sensory Evaluation

Agribusiness/ Agricultural Economics V Oct. 19 (Saturday) 11.30-12.30 Wacana Putra 2

AEC21-AEC24

AEC21 Mukhamad Najib Personal and Socio-Cultural Factors as Driving Forces of Indonesian Consumers’ Purchase Intention for Organic Food

AEC22 Tyrone Jasper I. Reodica

Comparative Supply Responses of Philippine Garlic and Ginger to Smuggling and Other Factors

AEC23 Kim Sun Ho Improving Livelihood of Ethnic Minority Households in Lao Cai Province of Vietnam

AEC24 Mirasol L. Agpuldo

Assessment of Rice Producing Organization in Lower Apayao, Philippines

Agricultural Chemistry


Presenter Title

Agricultural Chemistry I Oct. 18 (Friday) 16.15-17.15 Angsana Putra 1

AC01 Masaya Hojo Manufacturing process for new post-fermented persimmon leaf tea

AC02 James Kennard S. Jacob

Anti-Hyperglycemic Potential of Pleurotus djamor Mycelial Extract on Alloxan-Induced Mice (Mus musculus)

AC01-AC02

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Agricultural Extension


Presenter Title

Agricultural Extension I Oct. 19 (Saturday) 14.00-15.30 Wacana Putra 2

AEX01-AEX06

AEX01 Ryan Mark A. Ambong

Influencing Factors of Modern Rice Technologies Adoption Among Lowland Rice Farmers of Occidental Mindoro, Philippines

AEX02 Bunthan Sereirtoh

The Conditions of Developing Sustainable Rice Farming Through Agricultural Cooperatives-Case Study of Prey Kabbas District, Takeo Province

AEX03 Narat Rattanacharoen

Assessment of the Sustainability of Rice Cultivation Practice of Farmers in Three Certified Programs Compared with Conventional Farmers in Ubon Ratchathani Province, Thailand

AEX04 Agung Suryawan Wiranatha

Establishing Priority Criteria for Agritourism Development to Support Sustainable Tourism in Bali

AEX05 Torres, Remuel O Attitudes and Perceptions Toward the Edible Landscaping (EL) Technology: An Examination of The Cases of Rural Women and Elderly Groups in the Two Provinces in The Philippines

AEX06 Praleena Thongsri

The Development of The Elderly Potential in Urban Agricultural Center

Agricultural Extension II Oct. 19 (Saturday) 14.00-15.30 Wacana Putra 3

AEX07-AEX10

AEX07 I Gusti Ayu Oka Suryawardani

Structural Model of Rural Tourism Development in Bali to Buildup Global Competitiveness

AEX08 Nabara Isah Shehu

A Review of The Current Position of The Nigerian Agricultural Extension Service

AEX09 De Guzman, Rowena P.

Socio-Economic and Demographic Correlates of Edible Landscaping Technology Innovation Adoption: Implications for Community-Based Garden Projects

AEX10 Mohammed Galadima

Influence of Socio-Demographic Factors on Adoption of Improved Pearl Millet Production Practices in North-Eastern Nigeria

Agro-Engineering/ Agricultural Engineering


Presenter Title

Agro- AEG01 Al Christian R. Efficiency of Fabricated Pest Control Pilotless Engineering/ Hilario Aircraft in Commercial Poultry and Livestock Farms Agricultural in Oriental Mindoro Engineering I AEG02 Le Thi Giang Determination of Changes in Agricultural Land Use Oct. 18 (Friday) in Coastal Zone of Nam Dinh Province Using Gis and 16.15-17.15 Remote Sensing Technology

ISSAAS 2019


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Presenter Title

Angsana Putra 2 AEG01-AEG03

AEG03 Phan Thi Phuong Thao

Effect of Extraction Solvents on Quality of Vietnamese Tea (Camellia sinensis O. Kuntze) Seed Oil

Animal Science/ Animal Production


Presenter Title

Animal Science/ Animal Production I Oct. 18 (Friday) 14.30-16.00 Wacana Putra 3

AS01-AS06

AS01 Dwi Yuzaria The Livelihood Strategies of Dairy Farmers in The Town of Padang Panjang

AS02 Janjira Phavaphutanon

Chicken Microsatellite Markers Are Efficient to Assess Genetic Studies in Red Jungle Fowl and Great Argus

AS03 Nuraini Cocoa Pods Fermented by Pleurotus ostreatus in Quail Diet: Effects on Laying Performance and Egg Quality

AS04 Angie R. Poliquit Genetic Parameter Estimation in Reproduction Traits of Japanese Quail (Coturnix japonica Temminck & Schlegel, 1849) in The Philippines

AS05 Yose Rizal Effects of Fermented Cassava Leaf Meal and Palm Kernel Cake Mixture Inclusion in The Diet on Egg Characteristics and Egg-Yolk Fat Content of Kamang Ducks

AS06 Geradine C. Sanchez

Anticlastogenic Potential of Organic Turmeric (Curcuma longa L.) Aqueous Extract Against Mitomycin-C in The Bone Marrow Cells of White Mice (Mus musculus L.)

Animal Science/ Animal Production II Oct. 18 (Friday) 16.15-17.15 Wacana Putra 3

AS07-AS10

AS07 Jacob Anderson C. Sanchez

Molecular Diagnostics of Porcine Epidemic Diarrhea Virus (Pedv) Among Native Pigs in Pampanga Using Reverse, Transcription Polymerase Chain Reaction Assay

AS08 Khalil Mineral Composition, Physical Properties and Nutritive Values of Local Rock Flours for Feeding of Laying Quails

AS09 Yuliaty Shafan Nur

Technology in Processing Palm Press Fiber with Aspergillus niger To Improve Its Fiber Quality as a Component of Feed

AS10 Armenia Administration of Cassytha filiformis Butanol Fraction During The 3rd Period Pregnancy on Mice Is Relatively Safe To Its Ofspring Growth and Behavior Development

ISSAAS 2019


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Presenter Title

Animal Science/ Animal Production III Oct. 19 (Saturday) 8.30-10.00 Wacana Putra 3

AS11-AS16

AS11 Agerico L. Nicdao

Phenotypic Characteristics of Native Pigs Grown in Pampanga, Philippines

AS12 Hermon Cassava Leaves Suplement in Cow Diet of Ammoniated Rice Straw Basis and Synchronized in Releasing N-Protein and Energy in The Rumen

AS13 Manmueang Malison

Efficacy Test of Herbal Product for Protecting Fatty Liver Haemorrhagic Syndrome in Broilers

AS14 Akhis Soleh Ismail

Active Compounds and Antioxidant Activities of Gambier Water Draining (Uncaria gambier (Hunter) Roxb) Obtained from Different Processes

AS15 Phakka-orn Akaramathurakul

Effects of Multi-Enzymes on Productive Performance of Broilers

AS16 Maria Endo Mahata

Performance Of Laying Hens Fed Fermented Pineapple [Ananas Comosus (L) Merr] Peel

Animal Science/ Animal Production IV Oct. 19 (Saturday) 10.30-11.30 Wacana Putra 3

AS17-AS20

AS17 Jennifer B. Bermudez

Efficacy of Spray Dried Noni (Morinda citrifolia) on The Performance and Control of Coccidiosis in Eimeria spp. Infected Broilers

AS18 Mohammad Khurshed Alam

Food Safety Issue and Animal Welfare in Goat Marketing in Bangladesh

AS19 Karina Marie G. Nicolas

Establishment of Gut Health Management Protocol Against Gastrointestinal Parasites of Free-Range Philippine Native Pigs with Noni Fruit Aqueous and Spray Dried Extract

AS20 Adrizal Adrizal Performance of Growing Buffalo Bulls Fed Agricultural Wastes Sillage That Formulated by Fuzzy Linear Programming

Animal Science/ Animal Production V Oct. 19 (Saturday) 11.30-12.30 Wacana Putra 3

AS21-AS22

AS21 Arianne V. Julian Proximate Analysis and Antioxidant Activity of Sorghum bicolor L. After Solid-State Fermentation Using Hericium spp. and Stropharia rugosoannulata strains: A Potential Probiotic Ingredient of Animal Feeds

AS22 Agus Susilo The Physical Quality of Broiler Meat with Candlenut Powder (Aleurites mollucana (L.) Willd) as Feed Additive

ISSAAS 2019


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Aquatic Science


Presenter Title

Aquatic Science I Oct. 19 (Saturday) 11.30-12.30 Wacana Putra 3

AQS01-AQS02

AQS01 Shenna Kate M. Torres

Genetic Diversity and Population Structure of The Trevallies Caranx ignobilis and Caranx sexfasciatus

AQS02 Lea Concepcion B. Flores

Mushrooms on Aquaculture: Wild Mushrooms Against Fish Pathogens Streptococcus iniae and Pseudomonas aeruginosa

Crop Science/ Plant Science


Presenter Title

Crop Science/ Plant Science I Oct. 18 (Friday) 14.30-16.00 Auditorium

CS01-CS06

CS01 Trizhia Marie B. Silva

Corosolic Acid in Leaves of Banaba (Lagerstroemia speciose Linn.)

CS02 Sreeramanan Subramaniam

Micropropagation of Physalis Peruviana L. Using Shoot Tip and Nodal Segment Explants

CS03 Ida Ayu Astarini Developing New Potato Variety via Gamma Irradiation

CS04 Maria Fe. S. Bulao

Occurrence of Aflatoxin in Peanut in Central Luzon, Philippines

CS05 Lop Phavaphutanon

Root Distribution Pattern of Young Aromatic Coconut Trees

CS06 Anyamanee Auvuchanon

Vegetable Soybean Evaluation Using AFLP Markers for Breeding Program Utilization

Crop Science/ Plant Science II Oct. 18 (Friday) 16.15-17.15 Auditorium

CS07-CS10

CS07 Ghizan Saleh Performance, Stability and Heterosis Revealed by Locally-Developed Sweet Corn (Zea mays L. var. saccharata) Hybrids from A Multi-Locational Trial in Malaysia

CS08 Samuel C. Chukwu

SSRs for Marker-Assisted Selection of Resistance Genes Against Bacterial Leaf Blight and Blast Diseases of Rice

CS09 Parichat Buaban Physical Properties of Fruit Cuticle in Relation to Chili Anthracnose Reaction

CS10 Siraj Munir Bin Mohammad

Analysis of Growth of Oil Palm (Elaeis guineensis) and Physical Soil Properties of Sandy Coastal Soil Amended with EFB Compost Applied with Slow Release Fertilizer

ISSAAS 2019


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Presenter Title

Crop Science/ Plant Science III Oct. 19 (Saturday) 8.30-10.00 Auditorium

CS11-CS15

CS11 Hidehiko Kikuno Effects of Different Hot Water Treatments on The Storage Quality of Fruit on Mango ‘Irwin’ (Mangifera indica L.)

CS12 Nguyen Thi Bich Thuy

Effects of Harvesting Maturity and Temperature on Quality of Avocado Fruit cv. ‘Booth’ Fruits After

Storage CS13 Abubakar M.M Effect of Hot Water Treatments in Prolonging the

Shelf-Life of Differently Pollinated Rockmelon CS14 Mohd Norsazwan

Ghazali Oil Palm (Elaeis guineensis Jacq.) D × P Seed Germination and Morphophysiological Evaluation at The Nursery: A Comparison Between Seeds Differing in Colour

CS15 Rindang Dwiyani Shoot Production from Runner-Tip Culture of Strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-Induction Medium

Entomology

Session Info Paper ID Presenter Title Entomology I Oct. 18 (Friday) 14.30-16.00 Angsana Putra 1

ENTO01 I Kadek Wisma Yudha

Preference and Interspecific Competition in Encyrtidae Parasitoid on Invasive Pest, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudocococidae) on Cassava

ENTO01- ENTO05

ENTO02 Nina Maryana Diacrotricha fasciola Zeller (Lepidoptera: Pterophoridae) on Starfruit (Averrhoa carambola L.): Biological Aspects

ENTO03 I Wayan Supartha

Distribution and Structure of Invasive Pest Populations, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudocococidae) on Cassava in Bali, Indonesia

ENTO04 Dadang Toxicity of Extracts of Piper Retrofractum Fruit, Annona squamosa Seed, and Azadiractha indica Seed Against Tetranychus sp. (Acarina: Tetranychidae)

ENTO05 Ibrahim Sani Case Study: First Report of Thrips Infestation and Gray Mold Disease on Hydroponically Grown Lettuce in Perak Malaysia

Entomology II Oct. 18 (Friday) 16.15-17.15

ENTO06 Nurariaty Agus Preferences of Earwig, Euborellia Spp. (Dermaptera: Anisolabididae) As Pest Predators to Synthetic and Natural Artificial Feed

ISSAAS 2019


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Session Info Paper ID Presenter Title Angsana Putra 1

ENTO06- ENTO07

ENTO07 Vien Sartika Dewi

Micropropagation of Physalis peruviana L. Using Shoot Tip and Nodal Segment Explants

Forestry


Presenter Title

Forestry I Oct. 19 (Saturday) 8.30-10.00 Angsana Putra 1

FOR01 Maria Christina Zarate- Manicad

Evaluation of Syzygium lineatum in Apayao, Philippines For Its Utilization and Conservation

FOR01

Plant Pathology/ Plant Protection

Session Info Paper ID Presenter Title Plant Pathology / Plant Protection I Oct. 19 (Saturday) 8.30-10.00 Angsana Putra 2

PATHO01- PATHO06

PATHO01 Bernadith T. Borja

Development of Weather-Based Prediction Tools in Forecasting Tomato Leaf Curl Disease Epidemics in Northern Mindanao

PATHO02 Marita S. Panili ‘Candidatus Phytoplasma luffae’ 16SrVIII-A and Its Related Strain are Associated with the Cassava Witches’ Broom Disease in the Philippines

PATHO03 Safwan Mazlan Molecular Phylogeny of Bacillus pumilus Strains Causing Trunk Bulges Disease of Rubber Tree in Malaysia

PATHO04 Shun Tomita Susceptibility to Tolaasins on Tolaasin- Detoxyfying Microbacterium spp.

PATHO05 Elizabeth P. Parac

Characteristics of Two Selected Hybrids of Abaca (Musa textilis Nee) With Resistance to Bunchy Top

PATHO06 Baharuddin Characteristics of Bacterial Grain Rot on Rice in Indonesia

Plant Pathology / Plant Protection II

PATHO07 Mojica, Mary Joy P

Control of Coffee Berry Pathogen (Colletotrichum kahawae L.) Using Selected Botanical Extracts

ISSAAS 2019


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Session Info Paper ID Presenter Title Oct. 19 (Saturday) 10.30-11.30 Angsana Putra 2

PATHO08 Joanne A. Langres

Molecular Identification, Distribution and Chemical Control of Mango Gray Leaf Spot in The Philippines

PATHO07- PATHO10

PATHO09 Anysia Hedy Ujat

Fusarium Malaysia

Wilt of Local Banana Variety in

PATHO10 Kenji Yokota Promotion of Fungal Colony Elongation on Fusarium oxysporum by Bacterial Isolates from Soil

Plant Pathology / Plant Protection III Oct. 19 (Saturday) 11.30-12.30 Angsana Putra 2

PATHO11 Carla Suzuki Altrao

Analysis of The Inhibitory Effect of Bacterial Growth on Cabbage Leaves in Response to Treatment with Lipopeptides

PATHO12 Muhammad Ammar Abdul Rahman

Preliminary Diagnostic of Pantoea Strains Associated With Leaf Blight Disease Of Rice (Oryza sativa) Using Multiplex Pcr Assay

PATHO11- PATHO14

PATHO13 Tri Asmira Damayanti

Utilizing of Biocontrol Bacteria to Suppress Whitefly and Transmission of Cowpea mild mottle virus (CPMMV) on Soybean

PATHO14 Alysa G. Estopace

Endophytic Bacteria Associated with Healthy and Rust-Infected Coffea arabica L. Leaves

Plant Pathology / Plant Protection IV Oct. 19 (Saturday) 14.00-15.30 Angsana Putra 2

PATHO15- PATHO19

PATHO15 Earlyn Kate Grimares

Understanding the Quantitative Resistance of Rice to Sheath Blight Through Genotypic, and Histochemical Analyses

PATHO16 Tutik Kuswinanti

Cocoa Pod Rot in South Sulawesi: Their Characterization and Virulence Diversity

PATHO17 Abdullahi, Adamu

In Vitro Antimicrobial Activity of Ginger Essential Oil Against Selected Bacteria and Fungi

PATHO18 Maria Lima Diongzon Pascual

Next-Generation Sequencing Detects Emerging Begomoviruses Associated with Tomato (Lycopersicum solanum L.) and Pepper (Capsicum spp.) Crops in The Philippines

PATHO19 Suhanna Ahmad Detection of Six1 And Six8 Genes in The Banana Pathogen, Fusarium oxysporum f. sp. cubense from Peninsular Malaysia

Plant Pathology / Plant Protection V Oct. 19 (Saturday) 14.00-15.30 Angsana Putra 1

PATHO20- PATHO24

PATHO20 Ade Rosmana Control of Anthracnose Disease by Using Trichoderma asperellum in Combination with Composted Plant Residues and Other Fungi on Cacao

PATHO21 Mari Neila P. Seco

Resistance Evaluation and Gene Expression of Wheat-Durable Resistance Gene LR67 in Rice (Oryza sativa L.) Against Fungal Pathogens

PATHO22 Suryo Wiyono Diseases Control and Growth Promotion of Tomato by Application of Combination of Trichoderma hamatum THWSY013 and Plant Growth Promoting Rhizobacteria Under Field Condition

ISSAAS 2019


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Session Info Paper ID Presenter Title PATHO23 John Ericho R.

Quesada Effect of Light-Emitting Diode (LED) On the Mycelial Growth of Trichoderma longibrachiatum

PATHO24 Abdjad Asih Nawangsih

The Potency of Rice Phyllosphere Bacteria to Control the Bacterial Grain Rot Disease Caused by Burkholderia glumae

Plant Production/ Environmental Agriculture

Session Info Paper ID Presenter Title Plant Production/ Environmental Agriculture I Oct. 19 (Saturday) 10.30-11.30 Auditorium

PPEA01-PPEA04

PPEA01 Gulbuddin GULAB

Physiological Study on Tomato Fruit to Keep Freshness Under High-Temperature Condition

PPEA02 Brenda C. Panelo Varying Levels of Indole Butyric Acid (IBA) on Selected Forest Tree Cuttings

PPEA03 Weerasekara Mudiyanselage Indika Weerasekara

Soybean [Glycine max (L.) Merrill.] Seed Quality Produced Under Different Agro- Ecological Locations

PPEA04 Mary Yole Apple Declaro Reudas

Garlic (Allium sativum L.) Marketing Structure and Problems in Garlic Production in Looc and Lubang, Occidental Mindoro, Philippines

Plant Production/ Environmental Agriculture II Oct. 19 (Saturday) 10.30-11.30 Room I

PPEA05-PPEA08

PPEA05 Analita M. Magsino

Evaluation of Adlai (Coix lacryma-jobi) as Rice Substitute in Filipino Diet

PPEA06 Petcharat Netlak Association of Some Postharvest Quality Traits with The Genome Composition of Cultivated Bananas

PPEA07 Pattamawan Anusornpornpong

The Study of Senescent Spotting, Electrolyte Leakage and Anatomical of Peel in Banana During Ripening

PPEA08 Milagros R. Dumaslan

Field Performance of Strawberry (Fragaria x ananasa) Varieties Grown from Different Sub- Cultured Tissue Culture Meristem

Plant Production/ Environmental Agriculture III Oct. 19 (Saturday) 11.30-12.30 Auditorium

PPEA09-PPEA13

PPEA09 I Nyoman Rai The Effectiveness of Indigenus Endomycorrhizal Biofertilizer Prototype in Organic Salak (Salacca Zalacca) Plantations

PPEA10 Leoniel S. Bais Status of Farm Mechanization in Magsaysay, Occidental Mindoro, Philippines

PPEA11 Kantima Thongsri Effects of Gibberellin and Brassinosteroids Plant Growth Regulators on Seed Imbibition and Quality of Soybean Under Low Temperature

PPEA12 Ni Komang Alit Astiari

Effort to Increase Off-Season Production and Fruit Quality of Siam Orange (Citrus nobilis

ISSAAS 2019


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Session Info Paper ID Presenter Title var. microcarva L.) Through Application of

Mycorrhizal Inoculants and Auxin

PPEA13 Prakobkit Dangthaisong

Abiotic Stress During Flowering Stage Affected to Aromatic and Yield Components in Thai Fragrant Rice (Oryza sativa L.) Cultivars

Plant Production/ Environmental Agriculture IV Oct. 19 (Saturday) 14.00-15.30 Angsana Putra 1

PPEA14-PPEA18

PPEA14 Ahmad Junaedi Is The Rice Plant That More Efficient in Using Water Showing More Adaptive To Drought ?

PPEA15 Alcones, Ariel M Soil Quality and Biodiversity of Traditional Upland Rice Farms in Conner, Apayao, Philippines

PPEA16 Jamilu Halidu Genetic Diversity Studies in Okra A Bed Rock of Future Breeding Programme

PPEA17 Darel Kenth S. Antesco

Rooting Response of Glabrous and Pubescent Leaf Type Accessions of Pa’uohi’iaka

(Jacquemontia Sandwicensis A. Gray) Using Single and Four-Node Stem Cuttings

PPEA18 Mika Yoshida Physico-Chemical Responses to Storage Temperature of Calamondin (x Citrofortunella microcarpa) Fruit Packed in Perforated Polyethylene Film

Plant Production/ Environmental Agriculture V Oct. 19 (Saturday) 14.00-15.30 Auditorium

PPEA19-PPEA24

PPEA19 Itji Diana Daud Improvement of Corn Farming Income Through Endophytic Seeds in South Sulawesi Indonesia

PPEA20 Ted Dominique S. Belonias

Lead Tolerance and Antioxidant Activity of Andrographis paniculata (Burm.f.) Nees

PPEA21 Shahidullah Niazi Effects of Different Nitrogen Rates and Percentage of Shades on Growth and Yield Performance of Sweet Basil (Ocimum basilicum L.)

PPEA22 Yolina T. Castaneto

Vegetative Propagation of Lipote (Syzygium polycephaloides) Using Stem Cuttings in Different Rooting Media

PPEA23 NGUYEN Thi Thuy Hanh

Identification of QTLs For Nitrogen-Use Efficiency and Agronomical Traits Using Vietnamese Rice Landraces

PPEA24 Tessie A. Boncato Commercialization of Off-Season Tomato Production Technologies

ISSAAS 2019


12

Smart Farming/ Digital Agriculture

Session Info Paper ID Presenter Title Smart Farming/ Digital Agriculture I Oct. 18 (Friday) 14.30-16.00 Angsana Putra 2

SFDA01- SFDA05

SFDA01 Nik Norasma Che' Ya

GIS and Multispectral Imagery Ability on Rice Field Monitoring in Farming Management

SFDA02 Ramadhona Saville

A Study of Environmental Factors of Tomato Production in Green House by Utilizating Low Cost Sensor Network

SFDA03 Rhushalshafira Rosle

Monitoring Paddy’s Growth Using UAV. Paddy

Variety: PadiU-Putra SFDA04 R N Athirah Use of Normalized Difference Vegetation Index for

Paddy Management in Mobile Application Development

SFDA05 Arief Hartono Development of Quick Test Method for Soil pH, Nitrat, Available Phosphorus, and Exchangeable Potassium Combining Chemicals and Phone Cellular Application

Soil Science


Presenter Title

Soil Science I Oct. 19 (Saturday) 8.30-10.00 Angsana Putra 1

SS01-SS04

SS01 Marvin D. Cascante

Effects of Intensive Vegetable Production on The Properties of a Volcanic Soil (Andisol) in Leyte, Philippines

SS02 Jhon Vincent C. Caisip

Physiological Response of Carrot and Changes in Chemical Properties of Acidic Sandy Loam Soil Applied with Different Fertilizer Blends

SS03 Latief Mahir Rachman

Evaluation of Physical and Chemical Properties and Quality Assessment of Marginal Dryland Soil

SS04 Anass Rouifi Optimization of Compost Tea Extraction to Overcome Nutrient Deficiencies in Organic Farming System

ISSAAS 2019


13

LIST OF POSTER PRESENTATIONS


Presenter Title

POSTER SESSION Oct. 18(Friday) 16.00-16.15

Oct. 19 (Saturday) 10.00-10.30 12.30-14.00

PO01 Apichart Jai-aree Guidelines for Development of Organic Agricultural Product Marketing in Thailand

PO02 Machiko Kazami Effect of Soybean Whey on Physical Properties of Salmon Fillets

PO03 Yuichi Koshiishi Development and Characterization of 10 Novel Microsatellite Loci For The Emu (Dromaius Novaehollandiae) and Genetic Diversity of Japanese Farm Populations

PO04 Mina Orita Intramolecular Cyclization of A Bis Trichloroacetimidate, and It’s Application for The

Synthesis of Taxol Side Chain PO05 Yukako Ogawa Synthetic Studies on Bacilosarcins, Having Potent

Herbicidal Activity PO06 Natsumi

Nakamaru Development of A New Route for Intermediates of Carbapenem Antibiotics

PO07 Mana Adachi Arthrobacter sp. Isolate Enhance Fusarium Soil-Borne Disease

PO08 Nur Haida Syazana

Antioxidant Activities of Vernonia Amygdalina Leaves As Affected by Different Extraction Solvents

PO09 Shiho Tamaki Characteristics of Consumers of Ready-Made Meals Based on Eating Styles: Online Survey Targeting Tokyo and Osaka Residents

PO10 Wan Asrina Wan Yahaya

Distribution of Cu, Zn And Na in Merit Soil Series Cultivated with Maize

PO11 Izzah Abd Hamid Effect of Rainfall on Potassium in Sandy Loam Texture PO12 Wachiraya

Imsabai Impacts of Enviroment Factors on Fruit Cracking of Aromatic Coconut

PO13 Koki Asano Colonization of Roots in Sago Palm Seedlings Associated with Commercial

PO14 Hikari Obuchi Suppressive Effect of Plant Diseases Using Coffee Grounds

PO15 Kazuki YOSHIMITSU

Challenges for Introducing Foreign Labor in The Agricultural Labor Market in Japan And Future Prospects

PO16 Nurulhikma Md Isa

Overexpression of Ossap8 Enhanced Tolerance To Salinity Stress During Transition from Vegetative To Flowering Stage

PO17 Mitsuaki Maruyama

Functional Properties of Yam Leaves (Dioscorea spp.)

PO18 Ara Fatima C. Algar-Carbonera

Extraction and Comparison of the Β-Carotene and Lycopene Contents of Balbas Bakiro (Momordica conchinchinensis Spreng.) To Carrots, Squash, And Tomato

PO19 Takafumi Yoshida

Comparative Study of surfactin homologues on antibacterial activity

ISSAAS 2019


14


Presenter Title

PO20 Masami Gunji Functional Properties of Sea Buckthorn (Hippophae rhamnoides L.) Leaves

PO21 Pattraporn Nusit Effect of 1-Methylcyclopropene Powder in Controlling Longkong Fruit Drop

PO22 Wan Asrina Wan Yahaya

Extension Topics and Speaker Acceptance: A Cross Analysis Among UPMKB Staff

PO23 Ryan Rodrigo P. Tayobong

Yield, Lycopene and Beta-Carotene Content Of Buyok-Buyok (Momordica cochinchinensis Spreng.) in Response To Different Fertilizer Applications

PO24 Riko Morimoto Effect of Adhesion Properties of Gliadin on Extensibility of Wheat Flour Dough

PO25 Ryoka Tajima Physicochemical Analysis of The Rice Yamada Nishiki Used for Brewing Sake

PO26 Brian S. Santos Status Assessment of Channa sp. in The Philippines

PO27 Daisy May C. Santos

Sequence and Expression Analysis of Select Terpene Synthase Genes in Philippine Coffea liberica Varieties

PO28 Bee Lynn Chew The Effect of 1-Naphthaleneacetic-Acid (NAA) in Vitro Root Culture of Butterfly Pea (Clitoria ternatea L.)

PO29 Chiharu Nakashima

Detection of Colletotrichum gloeosporioides Species Complex Using A Loop-Mediated Isothermal Amplification Assay

PO30 I Nyoman Suarsana

Immune Response in Rats with The Intensive Training and Tempe Extract Supplementation

PO31 Jonathan Jaime G. Guerrero

Effects of Copper Oxide and Zinc Oxide Nano and Bulk Forms to Soil Fungal Community

PO32 I Wayan Nico Fajar Gunawan

Profile of Superoxide Dismutase by Immunohistochemical In Rats with The Intensive Training

PO33 Tuan Poy Tee Optimization Biogas Production from Anaerobic Co- Digestion Durian Shell with Cattle Manure Using RSM

PO34 Ron Patrick C. Campos

Fungi Associated with Corn Fields

PO35 Harold Ray R. Landingin

Optimization of Culture Conditions for Mycelial Growth and Basidiocarp Production of Pleurotus cornucopiae

PO36 Rose Ann B. Fernandez- Grospe

Calcium Fortification of Pleurotus florida Grown on Calcium –Enriched Rice Straw Based Formulation

PO37 Murni Karim A Preliminary Study on Characteristics of Enterococcus hirae Isolated from Seabass (Lates calcarifer) As Potential Probiotic

PO38 Renato G. Reyes New Mushroom – based Products with Natural Sources of Amino Acids: Mushroom Shing-a-ling and Chips

ISSAAS 2019


15


Presenter Title

PO39 Rita Muhamad Awang

Assessment of Yellow Sticky Traps Against Adult Diaphorina citri Kuwayama in Citrus

PO40 Tayobong, Rodrigo P.

Developing An Edible Landscape Garden Design In The Philippines Through The Community-Based Participatory Research (CBPR) Approach

PO41 Nurul Nadia Ramli

Consumers Intention To Purchase Korean Food Products

PO42 Norsazilawati Saad Detection of Begomoviruses In Chilli Plant In Malaysia

PO43 Erneeza Mohd Hata

In vivo Assessment of Streptomyces Treatment on Early Growth Development Of Oryzae sativa

PO44 Anis Syahirah Mokhtar

The Efficacy of Phaleria macrocarpa Crude Extract Against Leptocorisa oratoria, Sucking Insects of Rice

PO45 Evalour T. Aspuria

Greenhouse Acclimatization of In Vitro-Derived Aloe Vera (Aloe vera Burm., f.) as Affected by Different Potting Media

PO46 Nolila Mohd Nawi

Factors Influencing Consumer’s Awareness Towards

Powdered Camel Milk in Klang Valley PO47 Kosuke

Matsumaru Analysis Of The Metabolic Change In The Eker Rat

PO48 Kazuki Kuramae Functional Analysis of Tumor Suppressor Protein TSC1 Using Genetically Modified Animals

PO49 Norhayu binti Asib

Evaluation Study Of Moringa oleifera Extract Formulations Against Tribolium castaneum

PO50 Jonathan Jaime G. Guerrero

Identification and Siderophore Characterization of Fungi from Lipa Clay Loam Soil in The University of The Philippines Los Baños, Laguna, Philippines

PO51 Uma Rani Sinniah

Importance of Maturity Stage On Quality Of Lablab purpureus Seeds

PO52 Juju Nakasha Jaafar

Response of Adventitious Root Culture To Different Types and Strength of Media in Chlorophytum borivilianum (Safed Musli)

PO53 Aliyu Isah Take- tsaba

Competitive Ability of Aerobic Rice Genotype (MR219-9) Against Weed (Goosegrass) As Influenced by Biofertilizer Under Glasshouse Condition

16

ISSAAS 2019


ORAL ENTO01

PREFERENCE AND INTERSPECIFIC COMPETITION IN ENCYRTIDAE PARASITOID ON INVASIVE PEST, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudocococidae)

ON CASSAVA

I Kadek Wisma Yudha1 I Wayan Eka Karya Utama1

and I Wayan Supartha2* 1Magister of DryLand Agriculture, Faculty of Agriculture, Udayana University, Jln. PB. Sudirman

Denpasar 80231 Bali, Indonesia 2IPMLaB - Integrated Pest Management Laboratory, Faculty of Agriculture, Udayana University,

Jln. PB. Sudirman Denpasar 80231 Bali, Indonesia. *Corresponding email: [email protected]

Cassava mealybug, Phenacoccus manihoti Matile-Fererro (Hemiptera: Pseudococcidae) is an invasive pest that causes a lot of damage to cassava plants in Indonesia. The pest has now attacked cassava plants in Bali, but has never been reported previously. There are 3 types of parasitoids that regulate the population of cassava mealybug in the field, namely Anagyrus lopezi, Acerophagus sp and Blepyrus sp. This study aims to determine the preference of each parasitoid species to its host instar. The research also aims to evaluate the adaptability and parasitization ability of each parasitoid species to its host. The preference study used a randomized block design with 3 host instar treatments namely instar-2, instar-3 and adult with 10 replications. While the competition study used a randomized block design with 10 treatments namely P1, P2, P3, P1-P2, P1-P3, P2-P1, P3-P1, P1 + P2, P1 + P3 and P1 + P2 + P3 with 10 replications. (P1 = species A. lopezi, P2 = species Acerophagus sp and P3 = species Blepyrus sp). The treatments of P1, P2 and P3 are the release of each parasitoid species singly for 24 hours in a petri dish containing white ticks of P. manihoti instar-3. P1-P2 treatment is a single release of P1 for 24 hours and then replaced by P2 species for 24 hours. In the same way, the treatments for P1-P3, P2-P1 and P3- P1 are treated. While the P1 + P2 treatment is the exposure of both types of parasitoids simultaneously on a petri dish containing the host P. manihoti for 24 hours. In the same way, the treatment of P1 + P3 and P1 + P2 + P3 is also done. The variable that was turned off was the number of host parasites in each treatment. The preference level of each local parasitoid species is calculated using the Manly index. The results showed that each parasitoid species had different preferences and adaptability to the host instar. Anagyrus lopezi species have a strong preference for instar-3 hosts (Li = 0.42) which are also able to paralyze instar-2 hosts (Li = 0.09) and adults (Li = 0.02), while species Acerophagus sp and Blepyrus sp is only able to paralyze each instar-2 host (Li = 0.07) and instar-3 host (Li = 0.05) with very low index values. The competitiveness of parasitoid species, Acerophagus sp and Blepyrus sp differ markedly in parasitizing P. manihoti host, A. lopezi parasitoid is the most superior species among the three species, both single or double or triple species released simultaneously, that A. lopezi is the most powerful candidate for P. manihoti pest control in Indonesia.

Keywords: Preference, Interspecific competition, Braconid parasitoid, invasive pest, Phenacoccus manihoti, Cassava

Area of Discipline: Entomology

mailto:[email protected]

ISSAAS 2019


17

ORAL ENTO03

DISTRIBUTION AND STRUCTURE OF INVASIVE PEST POPULATIONS, Phenacoccus manihoti MATILE-FERRERO (HEMIPTERA: PSEUDOCOCOCIDAE) ON CASSAVA

IN BALI OF INDONESIA

I Wayan Supartha* and Dwi Widaningsih IPMLaB - Integrated Pest Management Laboratory, Faculty of Agriculture, Udayana University, Jln. PB. Sudirman Denpasar 80231 Bali, Indonesia.

*Corresponding email: [email protected]

Cassava mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), is a serious threat to cassava cultivation in many countries of the world. The pest has now entered Indonesia and attacked cassava plants with symptoms of bunchy top, which can cause death in plants. Symptoms of these pests have begun to appear in several locations of cassava plants in Bali. For this reason, in the first year of the study, it was focused on (1) ascertaining Phenacoccus pest species that attack cassava plants in Bali, (2) mapping the population distribution and abundance of these pest species on cassava plants in Bali, (3) determining the structure of pest populations in cassava plantations in Bali, (4) identify the dominant factors that influence the population structure of cassava mealybug in Bali. The results showed that the morphological and molecular characteristics of the mealybug species that attacked cassava in Bali were Phenacoccus manihoti. The species has spread widely to attack cassava plants in low and highland areas in Bali with a relative abundance that varies between districts. The highest population abundance is found in the districts of Bangli, Gianyar and Badung. The population structure of the pest species varies between districts / city in Bali. However, nymphs, instars-1, 2 and 3 dominate the pest population in almost all districts / city. Egg population is the lowest population structure among other stages. Total population density ranges from 24.48 - 293.48 egg, nymph and adult / shoots. The highest population density was found in the Bangli Regency area (293.44 egg, nymph and adult / shoots) while the lowest density was in the Karangasem district area (24.48 nymph an adult / shoots. The main factors affecting the population structure of P. manihoti in the field are intrinsic properties of pests such as the nature of fast breeding, polyphagic and cosmopolitan that are easily adapted to the environment and extrinsic factors such as plant factors (plant varieties, abundance of host plants in the field), how to grow crops (such as monoculture and mixed farming) and temperature and rainfall factors.

Keywords: Population Structure, Invasive Pest Phenacoccus manihoti, Cassava

Area of Discipline: Entomology

mailto:[email protected]

ISSAAS 2019


18

IPMLAB - FACULTY OF AGRICULTURE UDAYANA UNIVERSITY, OCTOBER 2019

Presented at ISSAAS International Congress & General Meeting 2019HELD 18 – 20 OCTOBER 2019, UNIVERSITI PUTRA MALAYSIA, SERDANG, SELANGOR

• Cassava is one of the staples food in Indonesia• Production of cassava in Indonesia, has fluctuated

and tends to decline from 2010 to 2015• One of the factors causing the decline in cassava

production is pest and disease disorders

• Phenacoccus manihoti Matile-Ferrero, is the most harmful pest in cassava plantations in many countries in the world (Belloti et al., 1999).

• The pest is an invasive pest originating from South America which has now spread to Indonesia and Bali.

Place and time

• This research was conducted in the field and laboratory.

• Field research was carried out in all districts in Bali Province, from March to July 2019.

• While the molecular analysis (PCR, DNA Squensing) of each Phenacoccus sp specimen was carried out in the Denpasar Class-I Plant Quarantine Laboratory.

Methods of Determining Location, Taking and Handling SamplesSampling locations were carried out in all districts in Bali Province on cassava plantations which became host plants for Phenacoccus sp.

At each location, samples were taken at an altitude of <500 m asl, and> 1000 masl in accordance with local site conditions.

Leaf sampling was carried out purposively as many as 5 shoots of cassava leaves per location in accordance with the conditions of availability of host plants in the field.

Leaf samples taken at each location are temporarily stored in a 2kg clear plastic bag, then collected in a cool box and then brought to the laboratory.

Samples were then taken 5 leaves and counted the number of pest populations and types of predators.

• Nymphs and adult of Phenacoccus sp were counted and recorded and accommodated in a collecting bottle containing 96% alcohol

• Identification of Phenacoccus sp was done by matching specimens with the morphological characteristics of Phenacoccus sp in accordance with the keys compiled by Williams and Granara de Willink (1992) (Figure 3.1).

• Molecular identification is done by preserving the fly specimens into pure ethanol then stored in the -20 C frezer.

• The preservation of these specimens is needed for DNA isolation, DNA electrophoresis, PCR chain reactions, which were then re-electrophoretic, before entering the final step of the screening method.

• DNA was visualized by horizontal electrophoresis using 1% agarose gel.

• After electrophoresis, DNA was visualized in a UV transiluminator.

• A good PCR product is followed by a screening phase. DNA screening is performed to determine the sequence of nucleotides in the COI region.

• The results of the screening are entered in the NCBI BLAST (Basic Local Alignment Search Tool) to see homology with the closest species.

• The phylogeny tree was obtained using the neighbor-joining method.

1. Population distribution records were carried out on plants that showed symptoms at all observation locations based on symptoms of attack that appeared and colonies consisting of nymphs, adult and egg sacs (Ovisac)

2. The abundance index was measured by the Margalefindex formula (Morrugan, 2004)

R1=𝑠−1

1𝑛𝑁

where: R1 : Abundance IndexS : Number of species foundN : Total number of individualsNilai : R1< 3,5 : Low

R1> 5,0 : Hight

The abundance index uses the Margalef index (Morrugan, 2004)R1=

𝑠−1

1𝑛𝑁

Where : • R1 : Abundance Index• S : Number of species found• N : Total number of individuals• Nilai : R1< 3,5 : Low

R1> 5,0 : Hight

Diversity Index of species - Shannon–Winner (Morrugan, 2004)

H’ = -∑iPln Pi

Where :

H’ : Diversity Indeks keragaman

Pi : Proportion of i-type

Nilai : H’<1,0 : Low; H’> 3 : Hight

Dominance index was calculated using the Menheinick index, (Morrugan, 2004)

D= ∑(𝒏𝒊(𝒏𝒊−𝟏)

𝑵 𝑵−𝟏

Dimana :

• D : Dominance Index

• Ni : individuals number of i-type

• N : Total number of individuals

• Values : D ≤ 0,00-0,30(low)

D ≥0,60 - 1,00 (Hight)

1. Morphological Identification of Phenacoccus sp. Lice (Left and Molecular (Right)

Analysis of P. manihoti White Lice Agarose Gel Found in Lowland (DR) and Highland Cassava (DT) Plants in Bali

Province

AB

Morphological characteristics of adult female Phenacoccus manihoti.

The reddish white color is covered by starchy secretions (A) and

special features on Photo preparations (B).

• The presence of P. manihoti white lice was found at all survey locations in nine regencies / cities in Bali.

• An indicator of the presence of P. manihoti on a host plant is indicated by the presence of a P. manihoti colony at the top of the plant consisting of pink nymphs, adult females and white ovisacs such as cotton

• P. manihoti's white mites spread from the lowlands (<500 masl) to the highlands (532 masl)

0100020003000400050006000700080009000

Popu

latio

n A

bund

ance

Regency/City ini Bali

Population Abundance

• The abundance of P. manihoti population on cassava

plants in each district has a different value.

• The highest population abundance was found in

Bangli Regency (7336 individuals), followed by

Badung (4535 individuals) and Gianyar (4203

individuals).

• While the lowest population abundance was found in

Karangasem and Jembrana regencies which were 612

and 716 respectively.

• The average population abundance of P. manihoti in

Bangli Regency ranges from> 100 -> 500 individuals;

while in Badung and Gianyar each> 100 -> 500

individuals and <100 -> 500 individuals.

• While the range of population abundance in

Karangasem and Jembrana districts is <100-500

individuals respectively.

P.

manihoti

Badung Bangli Buleleng Denpasar Gianyar Jembrana Klungkung Karangasem Tabanan

Total of Individuals / 25 Plant shoot

Telur 454 106 73 219 71 58 173 29 128

Instar-1 1105 3080 242 485 1312 198 454 197 608

Instar-2 1231 1568 431 251 1408 184 521 236 466

Instar-3 811 1301 395 153 1014 124 461 104 152

Instar-4 934 1281 163 233 398 152 360 46 260

0

5

10

15

20

25

30

35

40

45

50

Rel

ativ

e P

opul

atio

n A

bund

ance

(%

)

Regency/City in Bali

Age Structure telur Nimfa-1 Nimfa-2 Nimfa-3 Nimfa-4

• the population structure is dominated by instars-1 and 2 except in Buleleng Regency dominated by instars-3 and 4

• The eggs were found to be between 29 - 454 on 25 cassava plants.

• The mealybug nymph P. manihotiwas known to have a relatively high total number of individuals, with a range of between 46 - 380 tails / 25 shoots of cassava

• Population density in each location varies between 24.48 - 293.44 per plant shoot

• The highest population density was found in Bangli, followed by Badung, Gianyar, Klungkung, Tabanan, Denpasar and Buleleng

• The lowest population density is in Karangasem and Jembrana

181,40

293,44

52,16 53,64

168,12

28,64

78,76

24,48

64,56

0

50

100

150

200

250

300

350

Ke

pa

da

tan

po

pu

lasi

pe

r p

ucu

k d

au

n

Kabupaten/kota di Bali

telur Nimfa-1 Nimfa-2

Nimfa-3 Nimfa-4 total

Population of Mealybug P. manihoti per Shoots of Cassava Leaves in Bali

Regencies Variety of Cassava Altitude (m asl) Palnting Pattern Population Density of P.

manihoti (per 25 plant shoot

BangliUJ 5, Adira 1 danMalang 6

237-927Monoculture and

Intercropping198,92

Gianyar UJ 5, Adira 1 52-503Monoculture and

Intercropping116,6

Klungkung UJ 5, Adira 1 20-323 Monoculture 71,84Denpasar UJ 5 10-84 Monoculture 54,28

BadungUJ 5, Adira 1 danMalang 6


Intercropping163,24

Tabanan UJ 5, Adira 1 51-732 Monoculture 59,44Jembrana UJ 5, Adira 1 8-38 Monoculture 26,32

BulelengUJ 5, Adira 1 danMalang 6

17-375 Monoculture 49,24

KarangasemUJ 5, Adira 1 danMalang 6


Intercropping23,32

Regencies Rainfall (mm)

Tempe

rature

(0C)

Humidity (%)Wind velocity

(KmPh)

Bangli 39,6 - 143,7 28 - 30 73 - 79 7,5 - 9,1

Gianyar 39,6 - 143,7 28 -30 73 - 79 7,5 - 9,1

Klungkung 198,9 - 480,5 29 - 30 77 - 80 7,7 11,7

Denpasar 32,4 - 83,5 28 - 29 73 - 77 17,1 - 22,8

Badung 39,6 - 143,7 28 -30 73 - 79 7,5 - 9,1

Tabanan 39,6 - 143,7 28 - 30 73 - 79 7,5 - 9,1

Negara 69,6 - 214 30 - 31 74 - 79 8,7 - 11

Buleleng 565,6 - 711,2 26 - 27 79 - 81 7,7 - 10,9

Karangasem 198 - 480,5 29 - 30 77 - 80 7,7 - 11,7

• Factors that influence the structure of the Phenaccosu manihoti population in the field are intrisic and extrinsic factors.

• The main intellectual factor that influences the pest's adaptability is the availability of species and density of host plants in the field.

• As a polyphagic insect, it can attack various types of host plants in the field.

• Host plant factors such as abundance, variety also affect population structure

• The most influential physical factors are temperature and rainfall

• Natural enemy factors from predators have an influence on the

(a) Adult of C. montrouzieri , (b) Instar-3 of C. carnea Larva, (c) Adult of Scymnus sp.

Spesies PredatorBadung Bangli Buleleng

Denpasa

rGianyar

Jembran

aKlungkung Karangasem Tabanan

Total Individu (Ekor)

C. mentrouzieri 30 14 15 7 28 20 17 18 29

C. carnea 10 5 4 7 3 3 1 3 5

Scymnus sp 0 2 6 0 3 0 2 4 0

N Predator 40 21 25 14 34 23 20 25 34

R1 0,54 0,66 0,62 0,76 0,57 0,64 0,67 0,62 0,57

H' 0,56 0,84 0,94 0,69 0,59 0,39 0,52 0,78 0,42

D 0,62 0,49 0,42 0,46 0,68 0,76 0,72 0,54 0,74

1. Invasive mealybug species, which attacks cassava in Bali was Phenacoccus manihoti.

2. Mealybug pests have spread evenly to P. manihoti on cassava in Bali with high population abundance.

3. The pest population structure of P. manihoti was dominated by instar-1 and 2 nymphs except in Buleleng Regency with an average population density of 24.48 - 293.44 per plant shoot.

4. Host plant, altitude, temperature and rainfall were key factors that affect the structure and population density of P.manihoti in the field.

5. There were three predatory fauna that play a role in regulating the population of P. manihoti in the field, namely Cryptolaemus montrouzieri, Chrysoperla carnea and Scymnus sp.

• Test predatory functional responses to population densities of Phenacoccusmanihoti pests.

• Evaluate the demographic parameters of parasitoids and predators as natural enemies controlling mealybug in the field.

• Parasitoid Preference and Competition for Hosts

• Evaluate the biological potential of parasitoids and predators as biological agents for control of white Phenacoccus manihoti ticks on cassava in Bali.

Documents

ISSAAS 2019 - erepo.unud.ac.id