Influence of hyperactivity treatment at the collagenous tisue

7/29/2019 Influence of hyperactivity treatment at the collagenous tisue

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INFLUENCE OF HYPERACTIVITY

TREATMENT AT THE COLLAGENOUS

TISSUE DENSITY OF THE MUSCLEATTACHMENT OF LONG BONES

DURING GROWTH

NAME : ARIE PRADIPTA

NIM : 030.07.031


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TOPIC

Collagen is the major structural protein of the body

Approximately 33 percent of protein in the body is collagen

In fact, bone is also composed of collagen combined with certain minerals such as

calcium and phosphorus

Bone is a tissue composed of cells and is dominated by collagen extracellular

matrix (type I collagen) are known as osteoid.


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METHODS

A method for this study building on experimental animal with cross-sectional

approach

During the migration process, there are changes in the density collagen

tissue

As experimentals animal used 120 male rats

- Age 6 weeks- Weighing 70 grams

Doing the experiment in hyperactivity treatment during 2 until 6 months, may

be used to explain the results of primary research


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METHODS

Preparations for this experiment taken from musculus pectineus and

musculus rectus femoris from experimental animal, that is the muscle

attachment of the long bone during growth being checked under microscope

Preparations workable by the method of paraffin and made cuts nearly

parallel to the axis bone length and preparations 6 microns thick slices and

polished with Azan Mallory staining

On microscopic preparations of m. pectineus and m. rectus femoris

attachment assessed the level density of collagen tissue is qualitatively by

comparing the color of the collagen tissue with the guidelines previously

determined color density values with degree of color intensity values, ie

less dense (+) with a score of 1quite dense (++) with a score of 2

the densely (+++) with a score of 3, drawn from the entire preparation.


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METHODS

Three example of the level density can be seen in the picture below:

Figure 1: Microscopic images of longitudinal cross section of the

muscle tendon attachment on the bone density that show fibraecollagenous less dense. Mallory azan staining. Information: 1.Fibraecollagenouse 2. Cartilage cells


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METHODS



muscle tendon attachment on the bone density that show fibraecollagenous pretty solid. Mallory azan staining. Information:1.Fibrae collagenouse 2. Cartilage cells


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METHODS



muscle tendon attachment on the bone density showed a hiperdensity fibrae collagenous. Mallory azan staining. Information:1.Fibrae collagenouse 2. Cartilage cells


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METHODS

The Implementation Of The Research

All of the experimental animals are placed to live inside the cage with 90cm

long and 30cm height of size, for 10 rats in about 2 until 6 months after

treatment

Rats are fed with food 521 and added with 100gr mashed corns each day for

every 10 rats and also drink ad libitum

Experimental animals are divided in to two groups: 1. Controls (60 rats) 2.

Hiperactivity (60 rats)

The value of the collagen tissue density is taken at the M.pectineusattachment on femur proximal part and M.rectus femoris on tibia proximal

part, on the control group and hyperactivity group, each after 2 months and

6 months of treatment on the both group mentioned before


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METHODS

Measurement Result Of The Collagen Tissue Density On The Muscle

Attachment To The Bone

Table XIIa: The average of the collagen tissue density on control group 2months and 6 months

Time of

Treatment

The Average of Density Collagen Tissue

2 months 6 months

Muscles

Attachment

X S.B X S.B

M.pectineus 1,567 0,496 1,9330,249

M.rectus

femoris

2,600 0,554 2,8330,373


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METHODS

Table XIIb: The average of the collagen tissue density on hyperactivitygroup 2 months and 6 months

Time of

Treatment

The Average of Density Collagen Tissue

2 months 6 months

Muscles

Attachment

X S.B X S.B

M.pectineus 1,967 0,180 2,000 0,000

M.rectus

femoris

2,700 0,458 2,767 0,423


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METHODS

Table XIIc: Result of Fisher Exact test from the differences of the collagentissue density between 2 months and 6 months

Group Control Hyperactivity

Muscles Attachment

M.pectineus p>0,05*

*the differences not to shown


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METHODS

Table XIIc: Result of Chi-kuadrat test from the differences of the collagentissue density between 2 months and 6 months

Muscles

Attachment

Group

Control Hyperactivity

M.pectineus X2=10,755

p0,05*** p>0,05***

*the differences shown

**the differences very shown

***the differences not to shown


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RESULT

Hyperactivity treatment causes collagenous tissue density show fibrae

collagenous less dense, pretty solid, and hiper density

The addition of muscle activity during the development will increase the

growth of tendon.

In hyperactivity treatment (physical exercise) there is a correlation between

collagenous tissue density and the muscle attachment of long bones wassignificantly with the degree of migration of muscle attachment on the bone

during growth.


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RESULT

The treatment makes collagenous tissue density show fibrae collagenous:

Less dense

Pretty solid

Hiper density

Assessed the level density of collagen tissue is qualitatively by comparing

the color of the collagen tissue with the guidelines previously determined

color density values with degree of color intensity values, ie1. less dense (+) with a score of 1

2. quite dense (++) with a score of 2

3. the densely (+++) with a score of 3, drawn from the entire preparation.

In the hyperactivity treatment was found that the absolute color densityvalues with degree of color intensity values of the collagen tissue density at

the muscle attachment


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CONCLUSION

The influence of hyperactivity treatment for the experimental animal to get

the changes of collagenous tissue density of the muscle attachment of long

bones during growth.

It was found that in the hyperactivity rats, a change of collagenous tissue

density was detected, whereas none in the control group.

The change of the collagenous tissue density show fibrae collagenous lessdense, pretty solid, and hiper density.

In conclusion, there was a convincing the influence of hyperactivity

treatment at the collagenous tissue density of the muscle attachment of long

bones during growth. That influence to the muscules gain mass and will addthe sacroplasma. Hyperactivety treatment causes muscle hyperplasia or

hypertrophy that increases muscle volume and bone length change.


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REFERENCES

.

1. Boer A 1980 a. Pertumbuhan Femur dan Tibia Mencit akibat Perlakuan

Bipedal. Pertemuan Nasional Anatomi V Semarang. Boer A 1980 b.

Pertumbuhan dan Perkembangan Beberapa Otot Tungkai Belakang

Mencit akibat Perlakuan Bipedal. Pertemuan Nasional Anatomi V

Semarang.

2. Chaui R et al. 2004. A Little Exercise Goes A long Way. Child Health

Alert. Vol: 22, p: 2-3. National Library ofMedicines Midline.

3. Cheema TA et al. 2006. Measurement of Rotation of the FirstMetacarpal during Opposition using Comperted Tomigraph. Journal of

Hand Surgery. Vol: 31, Iss: 1, p: 76-9.

4. Cooper RR and Misol S 1970. Tendon and Ligament Insertion. A Light

and Electron Microscopic Study. J. Bone Joint Surgery 52A (1) : 1-20.

5. Dill DB et al. 1964. Handbook of Physiology, Adaptation to theEnvirontment. Section 4, pp.75. American Physiological Society.

Washington, D.C.

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Influence of hyperactivity treatment at the collagenous tisue