SUPPLEMENTARY INFORMAION

REGULAR ARTICLE

DNA Interactions and Biocidal activity of Metal complexes of Benzothiazole Schiff bases:

Synthesis, Characterization and Validation

NARENDRULA VAMSIKRISHNA, MARRI PRADEEP KUMAR, GALI RAMESH,

NIRMALA GANJI, SREENU DARAVATH and SHIVARAJ*

Department of Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India

Email: shivaraj_sunny@yahoo.co.in

*For correspondence

CONTENTS

1. 1H-NMR of L1 and L2 S12. IR spectra of L1, 1, 2 and 3 S23. SEM and EDX graphs of L2 and 4, 5 and 6 complexes S34. Mass spectra of L1, 1, 2 and 3. S45. Fluorescence spectra of EB (0 to 16.8 µM) bound to DNA (125 µM) S56. Viscosity of the EB, complexes 4, 5 and 6 S67. DNA cleavage patterns of complexes 4,5 and 6 S7

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Figure S1.

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Figure S2.

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Figure S3.

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Figure S4.

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Figure S5. Fluorescence spectra of EB (0 to 16.8 µM) bound DNA (125 µM); arrow shows the increasing emission intensity upon increasing concentration, maximum at [EB]=12.6 µM.

Figure S6.

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Figure S7. (a) Photoactivated cleavage of supercoiled pBR322 DNA (0.2 μg, 33.3 μM) by the complexes at 37 °C in 5mM Tris HCl/5 mM NaCl buffer UV irradiation of wavelength 345 nm. Lane 1, DNA control; Lane, DNA + L1 (20 μM); Lane 3, DNA + 4 (20 μM); Lane 4, DNA + 5 (20 μM); Lane 5, DNA + 6 (20 μM). (b) Oxidative cleavage of supercoiled pBR322 DNA(0.2 μg, 33.3 μM) at 37 °C in 5mM Tris HCl/50 mM NaCl buffer . Lane 1, DNA control; Lane 2, DNA +H2O2 (1mM); Lane 3, DNA + H2O2 (1mM) + L1; Lane 4, DNA + H2O2 (1mM) + 3 (20 μM); Lane 5, DNA + H2O2 (1mM) + 4 (20 μM); Lane 6, DNA + H2O2 (1mM) + 6 (20 μM).

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