Importance of trace minerals for nutrient stability in feed and optimized trace element supply of animals

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    Digital Re-print -March | April 2013

    Importance of trace minerals fornutrient stability in feed and optimized trace

    element supply of animals

    www.gfmt.co.uk

    Grain & Feed Milling Technology is published six times a year by Perendale Publishers Ltd of the United Kingdom.All data is published in good faith, based on information received, and while every care is taken to prevent inaccuracies,the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis ofinformation published.Copyright 2013 Perendale Publishers Ltd. All rights reserved. No part of this publication may be reproduced in any formor by any means without prior permission of the copyright owner. Printed by Perendale Publishers Ltd. ISSN: 1466-3872

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    Trace element supplementation

    to maintain optimal health and

    performance in livestock is often

    underestimated. This is partly due to the

    fact that trace elements perform very

    complex functions in the metabolism and

    deficiencies can lead to general disorders in

    animals and due to the important physiolog-

    ical functions it is therefore

    necessary to provide them to

    the animal through the feed.

    On the other hand, higher

    allowances for example of

    zinc and copper have also

    specific effects on intestinal

    health. To address the difficult

    discussion about physiology,

    intestinal health and finally

    environmental pollution too,

    the supply of trace elements

    in the EU is regulated by law

    (EU Regulation 1334/2003).

    Several years ago, the

    maximum concentrations

    in the feed were significant-ly lowered again to reduce

    the heavy metal output in

    the environment (Table 1).

    Impact onbioavailability

    It is known that the bio-

    availability of trace elements

    is difficult to compare due to

    the anatomy of the digestive

    tract between the different

    species. In addition, the trace

    element uptake capacity of

    the intestine decreases with

    increasing age of the animals

    in general. Furthermore, recent

    studies show that the trace

    element availability is dependent on health

    and also on the presence of stress. The trace

    element bioavailability, however, depends

    further on a number of factors such as

    performance stage, trace element status,

    form of the chemical bonds, trace element

    dosage and antagonists which inhibit absorp-

    tion (Flachowsky 2000). The lowering of the

    legal limits inevitably raises the question of

    trace elements with high bioavailability, and

    to understand even at lower concentrations

    in the feed the supply to the animals and

    also the possible additional effects at least

    partially.

    Important for absorption of, for example,

    zinc, manganese and copper are the mutual

    interactions as well as the die-

    tary amounts of iron, calcium,

    phosphorus, potassium and

    sulphur and further antago-

    nists. Unbalanced or high levels

    of some of these elements

    lead to shortages of the other

    elements. For example, a zinc

    surplus will cause copper trans-

    port across the enterocytes

    (intestinal epithelial cells) to be

    blocked due to the formation

    of intestinal metallothionein.

    This is also recognised as a

    secondary copper deficiency.

    Other factors affecting the

    availability of trace elementsare, for example, mycotoxins,

    crude fibre, tannins and phytic

    acid (Schenkel and Flachowsky

    2002).

    Characterisation anddifferentiation

    The various feed additives

    approved as trace element

    sources can be distinguished

    in terms of their availability for

    the animal. The best-known

    and largely used inorganic trace

    mineral sources of sulphates

    and oxides vary mainly due to

    their solubility. The main task

    of these trace mineral sources

    is to dissolve in the aqueous

    Table 1: Legal limits for trace elements in animal compound feed

    Traceelement

    Species

    Max. content incompound feed(mg/kgcompound feed)

    Copper

    Piglets until 12 weeks of age 170

    Bovine before the start of rumination 15

    Other bovines 35

    Ovines 15

    Crustaceans 50

    Other animals 25

    Cobalt All species 2

    Iodine

    Equines 4

    Dairy cows / Laying hens 5

    Fish 20

    Other animals 10

    Iron

    Piglets until 1 wee before weaning 250

    Ovines 500

    Pet 1250

    Other animals 750

    ManganeseFish 100

    Other animals 150

    Selenium All species 0,5

    Zinc

    Pet 250

    Fish / Milk replacer 200

    Other animals 150

    Importance of trace minerals for

    nutrient stability in feed and optimized

    trace element supply of animals

    by Dr Detlef Kampf, Orffa Additives, The Netherlands

    Gn&feed mnG tenooGy40 | march - pril 2013

    FEATURE

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    http://www.satake-group.com/
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    medium of the digestive tract and sulphates

    are attributed to a higher value than oxides.

    A product with very low solubility, for

    example, copper oxide, therefore should

    not be used (any longer) in feeding livestock.

    The good solubility of sulphates, however,

    provides us with the disadvantage that for

    example dissociated copper ions are very

    reactive (strong pro-oxidant that initiate and

    speed up oxidation) and therefore promote

    adverse reactions with feed components

    (degradation and damage of sensitive ingre-

    dients such as vitamins or fat as well as

    forming harmful free radicals and peroxides

    and reducing palatability of feed) (Miles et

    al. 1998; Lu et al. 2010; Pang and Applegate

    2006; Shurson et al. 2011).

    In contrast to inorganic, organically bound

    trace elements (trace element chelates) are

    stable in the diet and absorption by the

    animal is improved due to their chemical

    structure. Their advantage is thus superior

    in isolation from the solubility of inorganic

    trace elements, but also in reducing the

    absorption-inhibitory responses in the diges-

    tive tract. These are mostly antagonistic

    interactions with feed components that

    compete for the same absorption capabili-

    ties. The positive effects of organically bound

    to inorganic trace elements were confirmed

    in numerous scientific studies (Kincaid et al.

    1997, Nockels et al. 1993, Wedekind et al.

    1992). Nevertheless, an adequate assess-

    Graph 1: Impact of various copper sources on the

    concentration of vitamin E in feed (Lu et al. 2010)

    Graph 2: Impact of increasing dosages of various copper

    sources on the phytate phosphorus hydrolysis by phytase(Pang and Applegate 2006)

    Gn&feed mnG tenooGy march - pril 2013 | 41

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    ment of the quality of organically bound

    trace elements must be taken. It should be

    noted at this point that the products on the

    market significantly differ in quality that can

    be checked by simple analytical methods

    (Helle and Kampf 2008).

    Traditionally, minerals have been cat-

    egorised as either organic or inorganic. In

    2012 a new category of trace minerals,

    known as hydroxy trace minerals, obtained

    EU approval as a feed additive for all

    animal species. These new products (Dicopper chloride tri hydroxide Cu

    2(OH)

    3Cl

    and Zinc chloride hydroxide monohy-

    drate - Zn5Cl2(OH)

    8(H

    2O) brand name:

    IntelliBond) belong in the group of inorganic

    trace mineral sources, but have similar chem-

    ically stable structures such as organically

    bound trace elements. Covalent bonds and a

    unique crystalline matrix structure provide a

    reliable stability, which differentiates hydroxy

    trace minerals from other organic or inor-

    ganic minerals.

    In addition, there are major advantages

    from a nutritional point of view. In com-

    parison to copper sulphate, these products

    are virtually insoluble at neutral pH, which

    provides increased trace mineral stability in

    the intestine. Less reactive trace element

    ions occur in the upper digestive tract, which

    is associated with fewer interactions with

    other feed components. There are already

    numerous studies that demonstrate in com-

    parison to sulphates the better protection

    of feed components (Lu et al. 2010; Miles et

    al. 1998; Pang and Applegate 2006). Due to

    the crystalline structure the trace elements

    are slowly released over the full length of

    the small intestine resulting in more efficient

    trace element absorption and a very good

    effect on intestinal health. Trial results in all

    animal species confirm the superior effectscompared to sulphate (Allee et al. 2011; Luo

    et al. 2005; Miles et al. 1998; Spears et al.

    2004, Shao et al. 2010).

    Effects of various trace elementsources in feed

    Improved stability of sensitive feed ingredi-

    ents by using stable trace element sources e.g.

    chelates or hydroxy trace minerals in compar-

    ison to sulphates was already demonstrated in

    many trials (Miles et al. 1998; Lu et al. 2010;

    Luo et al. 2005; Banks et al. 2004; Liu et al.

    2005; Pang and Applegate 2006; Shurson

    et al. 2011; Kampf 2012). All these results

    are based on the non-hygroscopic and non-

    reactive (non-pro oxidative) characteristics of

    these trace element sources. In one example

    copper from copper sulphate at a high dosage

    level showed a negative impact on vitamin E

    stability in feed and in contrast, hydroxy cop-

    per was not negatively affecting the stability of

    vitamin E and showed comparable results as

    by using lower levels of copper sulphate (Lu

    et al. 2010; graph 1).

    In another trial increasing dosage levels

    of copper sulphate were tested in compari-

    son to other trace mineral sources on the

    phytate phosphorus hydrolysis by phytase In

    vitro. In this study copper sulphate has shown

    due to the high solubility and hence a highreactivity (formation of insoluble complexes

    with phytate) a strong negative impact on

    phytate phosphorus hydrolysis. In contrast,

    as well as the copper lysine complex and

    the hydroxy copper did not influence the

    phosphorus hydrolysis (Pang and Applegate

    2006; Graph 2).

    A recent trial was conducted to test the

    direct negative impact of copper from vari-

    ous copper sources and different dosages on

    the oxidative stability of soybean oil under

    Rancimat test conditions. In this study a

    strong negative effect of the copper dosage

    was observed. Beside this copper sulphate

    reduced the oxidative stability relatively by

    9 and 46 percent, in contrast hydroxy cop-

    per only by 5 and 36 percent respectively

    (Kampf 2012; Graph 3).

    Graph 4: Relative bioavailability of copper from hydroxycopper compared to copper sulphate in cattle (Spears

    et al. 2004)

    Graph 6: Solubility of zinc from hydroxy zinc and zincsulphate and impact on production of volatile fatty acids

    in the rumen (Schaeffer 2006)

    Graph 5: Relative bioavailability of zinc from hydroxyzinc compared or zinc sulphate in cattle (Schaeffer

    2006)

    Graph 3: Impact of increasing dosages of variouscopper sources on the oxidative stability of soybean

    oil (Kampf 2012)

    Gn&feed mnG tenooGy42 | march - pril 2013

    FEATURE

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    Effects in animalsMuch trial work has been carried out in

    monogastrics and in ruminants where stable

    trace element sources for example chelates or

    hydroxy trace minerals confirmed better effects

    when compared to sulphates with regards to an

    improved bioavailability, intestinal efficacy and gut

    health (Allee et al. 2011; Kidd et al. 1996; Luo

    et al. 2005; Miles et al. 1998; Nollet et al. 2008;

    Spears et al. 2004; Wedekind et al. 1992). This

    is based on the fact that stable trace element

    sources are, in contrast to sulphates, insoluble

    at neutral pH and will due to the covalent bond

    structure only partially dissolve in the acidic

    environment of the stomach. As a consequence

    more intact molecules will be available and effec-

    tive in the lower parts of the digestive tract, which

    results also in improved gut health.

    In one example in broilers a 13 to 16 per-

    cent higher bioavailability (expressed as tendon

    copper, 10 d deficient model, 0 - 6 ppm Cu)

    of hydroxy copper was measured in contrast

    to copper sulphate. In a second trial (same trial

    conditions) a 25 percent higher bioavailabilityfor hydroxy copper and 35 percent for copper

    chelate was measured (Klasing and Naziripour

    2010). Other research groups figured out a

    better relative biological value of organic zinc

    chelate by 1.64 (measured as tibia zinc content)

    compared with zinc sulphate as a reference zinc

    source (1.00) in broiler (Staret al. 2012).

    Not statistically proven but nevertheless

    numerically higher broiler tibia zinc concentra-

    tions especially in combination with a phytase

    supplementation was examined by Schlegel et

    al. (2010). In a recent meta-analysis the same

    authors reported that broiler bone Zn respond-

    ed slightly better (not at significant value) to

    organic zinc than inorganic zinc with increasing

    zinc levels (Schlegel et al. 2013). Noteworthy is

    the fact that in all these experiments in advance

    of the measurements no zinc deficiency situation

    has been created, whereby the positive effect of

    a more readily available source of zinc would

    have manifested better.

    In another example Allee et al. (2011)

    showed that increasing dosage levels of

    hydroxy copper from 15 to 200 ppm in

    piglets resulted in significant linear increases

    in average daily gain (ADG) and average

    daily feed intake (ADFI) at 7, 21, and 42days on test. Feed conversion ratio (FCR)

    was improved linearly by addition of hydroxy

    copper over the 42-day trial. In this trial

    the dose/response relationship of hydroxy

    copper on daily gain and feed conversion in

    piglets (0-42 days) resulted in a breakpoint

    of the maximum copper effect already at

    a dosage of 130-140 ppm (in comparison

    maximum copper effect of copper sulphate

    is at around 200-250 ppm) which indicates

    that hydroxy copper has a higher intestinal

    efficacy and is more valuable on gut health as

    copper sulphate (Allee et al. 2011).

    In ruminants the positive effects of organi-

    cally bound to inorganic trace elements were

    confirmed in numerous scientific studies and

    the major advantage was a better bioavailabil-

    ity compared to sulphates under the presence

    of stress or antagonists (Kincaid et al.

    1997, Nockels et al. 1993, Wedekind

    et al. 1992). In diets containing cop-

    per antagonists like molybdenum and

    sulphur a higher relative bioavailability

    of copper (based on plasma copper,

    plasma ceruloplasmin and liver copper)

    from hydroxy copper compared to

    copper sulphate was measured, too

    (Spears et al. 2004; graph 4). In another

    trial of the same research group the

    relative bioavailability of zinc from

    hydroxy zinc compared to zinc sulphate

    based on plasma zinc, and retained zinc,

    respectively, was also determined as

    better (Schaeffer 2006; Graph 5).

    In an additional trial the amount of

    soluble zinc was measured and com-

    pared with the production of volatile

    fatty acids in the rumen (Schaeffer 2006;

    Graph 6). Zinc sulphate delivered higher

    concentrations of rumen soluble zinc and

    showed a negative impact on the micro-

    bial fermentation (expressed as produc-tion of volatile fatty acids). In contrast zinc

    from hydroxy zinc was less soluble in

    the rumen and therefore not influencing

    the rumen microflora (Schaeffer 2006;

    Graph 6). This fact could be the explana-

    tion for a direct effect of trace elements

    on milk yield improvement in dairy cows.

    SummaryThe supplementation of trace elements

    plays an essential role in maintaining optimal

    health and performance of livestock. When

    considering the supply of trace elements

    not primarily the absolutely fed, but the

    usable amount of trace elements for the

    animal is crucial. This requires accurate

    knowledge of the composition of the feed

    and if possible, the diminishing availability

    of trace elements and interactions with

    other feed ingredients. The trace element

    supply can be improved by the use of

    high-quality trace mineral sources such as

    organic chelates, however, the assessment

    of the used quality is essential.

    Another new form of high-quality

    trace elements are hydroxy trace ele-

    ments, which recently received EUapproval and which are characterised by

    excellent stability and processing proper-

    ties together with a high physiological sig-

    nificance. The positive effects of chelates

    and hydroxy trace elements in feed (bet-

    ter stability of sensitive feed components

    and ingredients) as well as in animals

    (higher bioavailability) have been already

    confirmed in numerous publications.

    Literature is available upon request

    from the author.

    More inforMation:

    Tel: +31 183447771

    Email: [email protected]

    Website: www.orffa.com

    Gn&feed mnG tenooGy march - pril 2013 | 43

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