Identification and characterization of New Psychoactive …€¦ · Emma Gracia Lor. Identification and Assessment of New Psychoactive Substances: A European Network WS1 : Identification

Identification and characterization Identification and characterization

of of New Psychoactive New Psychoactive SubstancesSubstancesof of New Psychoactive New Psychoactive SubstancesSubstances

LisbonLisbon, 28th , 28th SeptemberSeptember 20162016

Emma Gracia LorEmma Gracia Lor

Identification and Assessment of New Psychoactive Substances:Identification and Assessment of New Psychoactive Substances:

A European NetworkA European Network

WS1 : Identification and characterization of NPSWS1 : Identification and characterization of NPS

Activity 1Activity 1: Listing NPS, collection of data, literature search. Start at month 1 and

last 6 months. This activity will produce a list of priority NPS already reported.

Activity 2.Activity 2. Identification of unreported NPS:

Activity 2a, by qualitative analysis of wastewater and urine from emergency rooms Activity 2a, by qualitative analysis of wastewater and urine from emergency rooms

and forensic samples, will start at month 3 and will last 1 year

Activity 2b, targeted survey, and analysis of wastewater and urine from music

festivals in Norway, will start at month 2 and will last 13 months

This activity will identify NPS use in the population, including substances not yet

reported, and will provide information for prevention strategies

Lisbon, 28th September 2016



Reports of new molecules sent by the EMCDDA

(December 2013-March 2016)

Data sources:

Activity 1Activity 1: Listing NPS, collection of data, literature searchListing NPS, collection of data, literature search


Alerts (Europe and Italy) + Italy seizure list +

Notifications in Italy (intoxications)

(October 2013-March 2016)

Database ~ Database ~ 350 NPS350 NPS

UNODC control list



Phenethylamine (37)

Arilcicloexilamine - ketamine anal. (3)

Piperidine (6)

Tryptamine (7)

Selection “Priority compounds”: 200 NPSSelection “Priority compounds”: 200 NPS

Criteria:

Frequency of use

Recently reported

Tryptamine (7)

Synthetic cathinone (53)

Synthetic cannabinoid (72)

Synthetic oppioid (9)

Aminorex derivate (3)

Natural psychoactive substance (4)

Benzodiazepine (4)







WS1 : Identification and characterization of NPSWS1 : Identification and characterization of NPS

Activity 2. Identification of unreported NPS:

Activity 2a, by qualitative analysis of wastewater and urine from emergency rooms

and forensic samples, will start at month 3 and will last 1 year




festivals in Norway, will start at month 2 and will last 13 months

This activity will identify NPS use in the population, including substances not yet

reported, and will provide information for prevention strategies.



Norway

Wastewater samples

(May 2016)

19 places

Alba-IuliaItaly

Switzerland

Portugal

Malta

Slovakia (4)

Netherlands

Slovenia

Bulgaria

Romania (3)

Poland

Sarajevo

Hungary

Serbia (2)

Ireland


Urine samples

(music festival –

August 2016)

Oslo



Wastewater samplesWastewater samples

Pooled sample: Friday + Saturday + Sunday

100 mL

(pH 7)100 mL

(pH 2)Oasis MCXOasis HLB


200 µL (10% MeOH)200 µL (10% MeOH)

Orbitrap QExactive

2 injections :

Screening (Full MS)

Confirmation (ddMS2)



Urine samplesUrine samples

5 mL Oasis HLB

Pooled sample from urinating spots

200 µL (10% MeOH)

Orbitrap QExactive

2 injections :

Screening (Full MS)

Confirmation (ddMS2)




Screening and identification of the compoundsScreening and identification of the compounds

Thermo hardware/software suites


Thermo hardware/software suites



Screening and identification of the compoundsScreening and identification of the compounds

TraceFinder application


TraceFinder application

Software able to do a rapid targeted

screening of compounds and identify

then according to different parameters

m/z Retention

time

Fragment

ions

Isotopic

pattern

Library

search

Library

score






1st injection: Screening (Full MS)1st injection: Screening (Full MS)

Wastewater

from Porto

23 “suspected”

NPS





Urine samples

from Norway


36 “suspected”

NPS







1st 1st injectioninjection: Screening (: Screening (FullMSFullMS))




2nd 2nd injectioninjection: : IdentificationIdentification and and confirmationconfirmation (ddMS(ddMS22))

4-Methylethcathinone?

NH

CH3

O

CH3

CH3


Exact m/z: 146.0964

(STD available)



2nd 2nd injectioninjection: : IdentificationIdentification and and confirmationconfirmation (ddMS(ddMS22))

ClCH3

N

O

4-chloro-αααα-PPP

Exact m/z: 238.1006��

Fragments (database)

m/z: 167.027 ?

m/z: 139.032��


Exact m/z: 238.1006��



Future workFuture work

Analysis with Orbitrap + data processing

Analysis of cathinones with QqQ


Sara CastiglioniEttore Zuccato Emma Gracia LorSara CastiglioniEttore Zuccato Emma Gracia Lor

Nikolaos Rousis Francesco Riva

Renzo Bagnati



Documents

Identification and characterization of New Psychoactive …€¦ · Emma Gracia Lor. Identification and Assessment of New Psychoactive Substances: A European Network WS1 : Identification