Grouping and compatibility issues and their resolution

3rd Basic Haematopathology CourseTMH

Mumbai

Discrepancy – A conflict or variation …between things that ought to be the same Collins dictionary

In the blood bank• Reverse typing discrepant with forward grouping• Patients phenotype inconsistent with antibody

profile• Group/type inconsistent with previously

assigned group/type• Crossmatch results discrepant with prior or

subsequent compatibility testing

Classification of grouping discrepancies

• Group I – Unexpected reactions in serum grouping due to weakly reacting or missing antibodies

• Group II – Unexpected reaction in cell grouping due to weakly reacting or missing antigens

• Group III – Unexpected reactions in cell and serum grouping caused by protein or plasma abnormalities

• Group IV – Miscellaneous problems

General principles for resolution

• Eliminate the chance of clerical error or misidentification by retesting where possible on a fresh sample.

• Test with alternate platforms and reagents • Use washed cells and saline

replacement/dilution to eliminate type III reactions

• Use enhancement techniques to bring out weak or missing reactions

• Review and analyse the patient’s history for a possible role

Lab tests that aid in resolutionThe tools to him that has the ability to handle them.

French proverb

• Multiple platforms and reagents• Antibody screening and identification• Non-routine serological tests

(enhancement techniques, secretor test, adsorption and elution, etc.)

• Extended phenotyping• Genotyping

Case 1 - Where a discrepancy aided diagnosis!

• A 55 year old lady slated to undergo an orthopaedic surgery elsewhere, was referred to our blood bank. Results of grouping done elsewhere were:-

Anti A Anti B Anti AB

A cells B cells O cells

4+ 4+ 4+ 2+ 2+ 2+

Forward group:AB Reverse group:???

• Rouleaux – Stacking of erythrocytes in a coin like fashion (right) can be mistaken for agglutination (left)

• Saline dilution/replacement will neutralise rouleaux formation

• Microscopy: Rouleaux

• With saline dilution reverse group consistent with AB.

• In view of the clinical history of fracture, patient’s clinician informed of need to exclude plasma cell dyscrasia.

• On further investigation, patient diagnosed to have myeloma

• The presence of a discrepancy may point to a significant clinical condition

Case 2 – Clinical history to the rescue!

• Sample from a 1 year old child was sent for grouping

• Grouping results

Anti A Anti B Anti AB

A cells B cells O cells

Neg Neg Neg Neg Neg Neg

Forward group:O Reverse group: AB?

• Sample retested after incubation at 4 degrees Celsius showed similar results.

• Clinical history enquired into:- history of recurrent infections; suspected case of severe combined immunodeficiency syndrome

• Inference: Serum grouping negative because of hypogammaglobulinemia.

• Clinical history can play an important role in resolving a discrepancy

Case 3 – A blood group makeover!

• 59 year old gentleman posted for surgery for carcinoma colon.

• Previously documented A group• Repeat grouping:-

Anti A Anti B Anti AB

A cells B cells O cells

4+ 2+ 4+ Neg 4+ Neg

Forward group: AB Reverse group: A?

• Patient’s serum tested against patient’s own cells gave a negative reaction

• Acquired B suspected in view of the above, and clinical history

• Confirmed by secretor test: Showed only anti A

• Inference: A group with acquired B

Acquired B phenotype

• Seen mostly in patients with gastrointestinal lesions, particularly carcinoma colon.

• Always occur in group A patients, nearly always A1

• Acquired B cells do not agglutinate with patient’s own anti B

• Secretors do not secrete B substance• Reversible on treatment with acetic anhydride• Ph sensitive:- Anti B reagents with PH beyond

the range of 6.0-8.5 do not agglutinate acquired B cells

oOOO

OH

CH2OH

O

Bacterial deacetylating enzymes

A immunodminant sugar(N- acetyl D galactosamine)

Acquired B (D galactosamine)

OH

CH2OH

OH

O

OH

OH

OH

B immunodominant sugar(D galactose)

O

OH

OH

CH2OH

NHCO- NH2

Bacterial deacetylating enzymes

OH OH

O

Anti B

Case 4 – An argument in favour of different phases of compatibility testing!

• A 19 year old youth posted for an elective surgery was grouped in our blood bank. The patient had received 16 uneventful transfusion prior to this

• Grouping results

Anti A Anti B Anti AB A cells B cells O cells

4+ 4+ 4+ Neg Neg Neg

Forward group:AB Reverse group:AB

• Antibody screen - Negative

Crossmatch results

Phase of testing

Donor 1Group AB

Donor 2Group A

Donor3Group O

saline Neg Neg Neg

37o C 3+ 3+ Neg

Coombs 4+ 4+ Neg

Patients cells tested with A1 lectin – Negative reaction signifying A2B group

Incompatible units tested with lectin and found to be positive signifying A1 phenotype.

Crossmatch performed with A2B unit was compatible

Inference: A2 with anti A1

• Significance:- Anti A1 reactive at 37o C – clinically significant!!

• Would have been missed if immediate spin crossmatch alone was done eg. type and screen/electronic crossmatch scenario

Case 5 – Dilemma after resolution!

• 55 year old lady slated for elective surgery referred from elsewhere. She had received 2 uneventful transfusions of O positive blood elsewhere one year previously.

• Grouping performed elsewhere.

Anti A Anti B Anti AB

A cells

B cells

O cells

Neg Neg Neg 4+ 4+ NegForward group: O Reverse group: O

Case 5 – Dilemma after resolution!

• 55 year old lady slated for elective surgery referred from elsewhere. She had received 2 uneventful transfusions of O positive blood elsewhere one year previously.

• Grouping performed elsewhere.

Anti A Anti B Anti AB

Anti H

A cells

B cells

O cells

Neg Neg Neg Neg 4+ 4+ Neg

Our groupingPhase

Anti A

Anti B

Anti AB

Anti H

H lectin

A cells

B cells

O cells pooled

O cells donor1

O cells donor2

O cells donor 3

IS/LISS

Neg Neg Neg Neg Neg 4+ 4+ Neg Neg Neg Neg

4O inc

Neg Neg Neg Neg - 4+ 4+ Neg Neg Neg Neg

37O inc

Neg Neg Neg Neg - 4+ 4+ Neg Neg Neg Neg

Coombs

Neg Neg Neg Neg - 4+ 4+ Neg Neg Neg 2+

Lewis typing: Le(a-b+) suggesting secretor positive status

Inference: Parabombay (Oh secretor)

Bombay categoriesClassification

Gene Glycosyltransferase

Antigens Secretion

Antibodies

Bombay (Red cell H deficient, non secretor)

hh seseA and/or B

None or A and/or B (depending on ABO genotype) in RBC or serum

None None Anti-A, anti-B, anti-H

Parabombay(Red cell H partially deficient, non secretor)

hh (weak variant)sese

A and/or B (depending on genotype)

Weak A and/or B depending on genotype. Weak H in Oh.Residual H if A or B enzymatically removed in others

None Anti H, anti A and/or anti B (depending on ABO genotype)

Parabombay(Red cell H deficient secretor)

hhSe

A and/or B(depending on genotype), H in serum

Weak A and/or B depending on genotype and weak H

H, A and/or B

Weak IH, anti-A and or anti-B

• H deficient secretor• Little or no A,B and H antigens• RBCs may be agglutinated by strong anti-H.• Usually not agglutinated by anti-A and anti-B but may

occasionally be agglutinated by potent antisera or anti-AB

• Weak H like antibody (HI) reactive at low temperature almost always present in sera. This is not inhibited by secretor saliva and does not agglutinate cord cells.

• Normal H levels in saliva

Para-bombay (Oh secretor)

Ulex europaeus (common gorse)

• (In red cell H deficient secretors) ‘Anti-HI is unlikely to be active at 37oC. ABO-identical blood, compatible by ICT at 37oC, can be used for transfusion.’

• (In H partially deficient non secretors) ‘little information exists on the clinical significance of anti H. Ideally Oh (Bombay) phenotype should be selected, but if not available red cells of the appropriate ABO group (A for Ah, B for Bh) compatible by ICT at 37oC, may be used.’

Source: The clinical significance of blood group alloantibodies and the supply of blood for transfusion. (NHSBT specification SPN214/1.1)

Bombay blood group not available! Can I transfuse O group cells in Parabombay

phenotype?

Case 6 – Transfusion and transplantation

• 39 year old male with Chronic Myeloid Leukaemia

• Underwent bone marrow transplant using stem cells from HLA identical sibling

• Blood group of patient – AB positive• Blood group of donor – O positive• Received 2 units of compatible O+ve red

cells in CMC, 6 days and 3 days prior to transplant

• 1 day after transplant blood request for 1 unit packed cells received.

• Forward typing of patient sample – AB+ve• Backward typing of patient sample –AB+ve • Antibody screen – Negative• Issued 1 unit of O positive blood

crossmatched and compatible till Coombs

• 1 day after transfusion haemoglobinuria reported.

• Major ABO incompatibility between donor and recipient

• Minor ABO incompatibility between donor and recipient

• Major incompatibility for other blood group antigens• Minor incompatibility for other blood group antigens• Transfusion of erythrocytes incompatible with

donor or patient• Other causes of immune haemolysis eg. auto

immune haemolytic anaemia, drug induced• Non immune haemolysis - TTP

DD of haemolysis following haematopoietic stem cell transplantation

Major incompatibility between donor and recipient

• Due to recipient-derived antierythrocyte antibodies

• Haemolysis immediately after transplant

• Should be suspected when DCT positive

• Eluate must be tested for anti A and anti B to identify haemolysis due to ABO mismatch

• Volume of erythrocytes in bone marrow HPC product can be equal to or greater than that of a unit of blood. Therefore, significant haemolysis is possible.

• Erythrocytes can be removed from the donor's bone marrow by Hetastarch separation, mononuclear cell concentration by machine, or through density gradient separation.

• During apheresis collection, haematocrit should be kept to less than 2%

• Other methods of prophylaxis include plasma exchange using AB plasma or in vivo adsorption using donor group plasma blood group substances; carry risk of rebound and haemolytic transfusion reactions

Minor incompatibility

• Usually delayed haemolysis• May be due to plasma or ‘passenger

lymphocytes’• Plasma in a bone marrow product to be

depleted depending on agglutinin tire. • Diagnosis - DCT positive with eluate

showing anti A or anti B

Passenger lymphocyte syndrome

• Donor derived lymphocytes in the HPC graft form blood group specific antibodies to patient erythrocytes

• Usually ABO antibodies but can involve other blood group antibodies. (Kell, Kidd, Duffy reported)

• Risk factors inclcude treatment with cyclosporine alone without an antproliferative agent like methotrexate, low intensity conditioning and ?PBSCT (theoretically, since it contains more B lymphocytes)

• Usually begins 1-2 weeks after transplant, persists for 5-10 days, then subsides

• Haemolysis can be severe• Can involve transfused compatible erythrocytes (bystander

immune haemolysis) • Management - Transfusion of compatible erythrocytes at a pace

that matches haemolysis• In massive haemolysis with risk of renal injury, exchange

transfusion may be considered

Incompatibility due to other blood group antibodies

• Supected when DCT is positive and antibody screen positive for donor or recipient

• The eluate shows an irregular antibody

Investigations in our patient

• DCT +ve (2+) • Post transfusion sample – ICT +ve; antibody

screen positive (1+)• Antibody ID – anti Jkb (3+)• Transfused red cells – Jkb positive • Phenotype of stem cell donor- D+; C4+; E-;

c4+; e4+; K-; Fya3+; Fyb -; Jka3+; Jkb3+• Phenotype of patient – D+; C4+; E-; c4+;

e4+; K-; Fya3+mf; Fyb 3+mf; Jka3+mf; Jkb3+mf

• Inference: Delayed haemolytic transfusion reaction due to anti-Jk(b) formed by patient reacting with transfused cells.

• Subsequent transfusions with Jkb negative fully compatible units.

• However, haemoglobinuria persisted until 54 days after transplant, when antibody screen turned negative.

Pure red cell aplasia

• Occurs in major mismatch• Recipient lymphocytes may remain and later produce

antibodies to transplanted donor derived erythrocytes• Can occur early or late (>100 days)• Suppress erythropoiesis by destroying erythroid

progenitors• Diagnosis – Based on reticulocytopenia persisting for

more than 60 days with absence of erythroids in marrow • Delayed erythrocyte engraftment occurs in 20% of ABO

mismatched transplants• ?related to haemagglutinins

Transfusion support in mismatched transplants

• Must be compatible with recipient and donor

• Such compatible blood must be selected prior to transplantation (from the start of myeloablation)as red cells may persist for weeks

3 phases of transplantation

Phase 2Phase 1 Phase 3

Beginning of preparationRBC tx:DCT negative and recipient isoagglutinins not detectable

Plasma and platelets tx: Recipient red cells not detectableRecipient group

Donor group

Compatible with both donor and recipient

Transplant

Selection of blood products in phase 2

Recipient RBC group

Donor's RBC group

Category of ABO mismatch†

Erythrocyte transfusion

Platelet or plasma transfusion

A O Minor O A, AB

B O Minor O B, AB

AB O Minor O AB

AB A Minor A, O AB

AB B Minor B, O AB

O A Major O A, AB

O B Major O B, AB

O AB Major O AB

A AB Major A, O AB

B AB Major B, O AB

A B Minor and major O AB

B A Minor and major O AB

A A None A, O A, AB

B B None B, O B, AB

AB AB None AB, A, B, O AB

O O None O O, A, B, AB

Blood group chimerism

• Forward or backward grouping discrepancies and mixed field agglutination

• Intrinsic characteristic of mismatched transplantation eg. A group recipient transplanted with O group donor

Anti-A

Anti-B

Anti-AB

A cells

B cells

O cells

Inference

MF Neg MF Neg 4+ Neg Mixed field on front type may be due to transfused O cells/ conversion. Recipient red cells still seen. Reverse type is recipient group

Neg Neg Neg 2+ 4+ Neg Front and reverse type donor group, indicating full donor engraftment

Case 7 – ABO mismatch in kidney transplant

• A 21 year old gentleman of blood group O-positive, with end stage kidney disease

• Only available related donor was mother whose blood group was A-positive.

• HLA-crossmatch by Complement Dependent Cytotoxicity (CDC) was negative

• Anti-A titre at presentation was 1:512.• Desensitization protocol involving Rituximab,

plasmapheresis and Anti-thymocyte globulin initiated

• Column agglutination test used for monitoring• Schedule of monitoring: Pre-pheresis, immediately post

pheresis and 24 hours later to detect rebound• A group FFP used for replacement in order to quench

anti A in recipient. • Rituximab interfered with HLA CDC crossmatch. HLA

antibody monitoring supplemented with solid phase assays

• Anti A titre reduced to 1:2 against which transplant performed successfully.

• 3 years post transplant - graft functional, patient well

A brief history of ABO incompatible kidney transplant (ABOi KT)

• 1955 - earliest efforts by Chung et al. 8/ 10 grafts failed within few days

• 1987 – Thielke et al transplanted A2 grafts into O donors. 12/20 retained long term function

• 1987- Alexandre et al used plasmapheresis in protocol and achieved 75% 1 year graft survival

• From 1989 popularised in Japan (near absence of deceased donors and only 0.15% A2). 14 % of all transplants in Japan.

• From 2000, gained acceptance in western countries• Today, outcome not different from other transplants*

*J. M. Gloor and M. D. Stegall, “ABO incompatible kidney transplantation,” Current Opinion in Nephrology and Hypertension, vol. 16, no. 6, pp. 529–534, 2007. 

Principles of ABOi KT

• Removal of existing antibody – plasma exchange, double filtration plasmapheresis, immunoadsorption

• Preventing recurrence of antibody – IVIg, immunosuppression, splenectomy/ Rituximab

• Waiting until titres achieve safe levels for transplantation

Range of Plasmapheresis Treatments required Before and After ABOiKT based on initial antibody titre

Lipshutz GS et al. Arch Surg. 2011;146(4):453-458

Monitoring of antibody titre

• Baseline antibody levels (> 256) predict severity of antibody mediated graft injury and graft survival. Reports suggest this is no longer true in patients that received tacrolimus or mycophenolate mofetil for immunosuppression*

• Titre cutoff for transplant 16– empirically chosen • Titre endpoint based on ?IgG or ?IgG and IgMB.

* H. Chung, J. Y. Lee, S. H. Kang et al., “Comparison of clinical outcome between high and low baseline anti-ABO antibody titers in ABO-incompatible kidney transplantation,” Renal Failure, vol. 33, no. 2, pp. 150–158, 2011.

Possible outcomes of ABOi KT

• Rejection (2-5%)• Immunological tolerance – ABO antibodies

against graft do not reaccumulate. ?an outcome of prolonged B cell/ T cell suppression

• Accomodation – ABO antibodies recur, but the graft is not rejected. Due to ?change in quality of antibody eg. shift in IgG isotype ?change in allograft eg. change in balance of apoptotic proteins bcl-2 and bcl-xl, up regulation of complement inhibitory substance CD59

If we cannot end our differences, at least we can make the world safe for diversityJohn F. Kennedy

Acknowledgement : Dr. Mary Purna ChackoAsst. Prof: Dept of Transfusion Medicine