GMS provides “One stop solution”Sacace Biotechnologies Srl

“Complete qPCR Solutions”

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Founded in 2001 Sacace Biotechnologies s.r.l., is an innovative Italian

company, located in Como, Lombardia

2001-2009: Caserta, Campania region Now: Como, Lombardia region

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Our MissionTo supply the best products to the

international diagnostics and pharmaceutical industry, as well as to hospitals and laboratories

through a global network of distributors located in more than 60 countries all over the world.

Sacace Worldwide

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Functions• Research & Development• Manufacturing• Logistics• Marketing• Certifications (ISO 9001:2008

13485:2012, CE Mark - Directive 98/79 EC)

• International external quality control

Hepatitis Viruses (Quantitative tests for HCV, HBV, HDV, HGV, HAV detection)

Human Papilloma Viruses (Screening, Typing, Quantitative detection of Low & High Risk genotypes)

STD (Multiplex detection of Chlamydia, Neisseria, Trichomonas, Mycoplasma, Treponema, Candida ….)

Herpes Viruses (Quantitative Multiplex detection of CMV, EBV, HHV6, HSV, VZV)

Our PCR Kits

TORCH infections (Toxoplasma, Rubella, Parvovirus)

Respiratory infections (Tuberculosis, Influenza, hRSV, Bordetella, Pseudomonas, Legionella)

Neurological infections (Enteroviruses, N.meningitidis, H.influenza, Str.pneumoniae)

Gastrointestinal infections (Shigella, Salmonella, E.coli, Rotavirus, Astrovirus, Norovirus, H.pylori)

Our PCR Kits

Single Nucleotide Polymorphisms (SNP):

• Cardio Trombophilia Panel (FII, FV, FVII, FXIII, FGB)

• Cardio Hypertension Panel (ADD, AGT, NOS....)

• Folates Methabolism Panel (MTHFR, MTR, MTRR)

• Haemochromatosis Panel (HFE 187, HFE193,

HFE845)

• Warfarin Panel (CYP2C9, CYP4F2, VKORC1)

Our PCR Kits

Oncologic diseases (Mbcr-ABL, BRCA)Genetically Modified Organisms (Soya,

Corn, Rice, Tomatoes)Veterinary kits (CPV, PPV, CSVF,

BVDV, ASF, Brucella, TGEV...)RNA/DNA extraction kits

Our PCR Kits

Our Real Time PCR kits can work on multiple instruments:

Qiagen Rotorgene TM

Bio-Rad iQ5TM

Cepheid SmartCycler TM

Applied Biosystems 7300/7500/StepOne TM

Stratagene MX3005P TM

Roche LightCycler 2.0 TM

Bioer LineGene-K TM

SaCycler-96 TM from Sacace Biotechnologies ...more to come

Our PCR Kits

SaCycler™-96

SaCycler™-96 Features

4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well

Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips

Possibility to use tubes with different heights with regulation of the matrix block

Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera

Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance

SaCycler™-96 Features

Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings

Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis

Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming.

SaCycler™-96 Characteristics

Thermal block format 96 test tubes of 0.2 ml (12 х 8) Test tube type 0.2-ml test tubes for PCR (individual, in strips, 8 pieces each or a

holder 12 x 8) Range of thermal block temperature control 0 C…100°С Resolution of temperature setting 0.1°С Absolute accuracy of temperature maintenance ±0.2°С Uniformity of thermal block temperature ±0.15 °С Maximum heating rate of the thermal block within temperature range of 4…99

°С 3.5 °С /s Maximum cooling rate of the thermal block within temperature range of

99….55 °С 2.5°С /s “Hot cover” temperature 105°С 1°С Actuating device of the thermal block Peltier elements Excitation source Light-emitting diode (LED) Detector CCD (charge coupled device) -matrix Number of the fluorescence measurement channels 4 or 5 Excitation/detection wave length 470/525, 532/570, 585/633, 633/670, 690/750

SaCycler™-96 Comparison  ABI 7500

BioRad CFX96

Qiagen RotorGene Q SaCycler-96

Thermal System Peltier Peltier air cooled PeltierAverage ramp rate (°C/s) 1.6 3.3 15-20 3.3Temperature accuracy (°C) ±0.25 ±0.2 ±0.25 ±0.2Uniformity of thermal block temperature (°C) ±0.5 ±0.4 ±0.01 ±0.15Range of temperature (°C) 4-100 0-100 ambient-99 0-100 

Optical SystemTungsten halogen

lamp LED LED LEDExcitation spectrum 5 filters 450-684 nm 365-680 470-750Detector CCD camera Photodiodes PMT CCD cameraEmission filters/channels 5 6 6 4 or 5

Compatible dyes

FAM, JOE, VIC, NED, TAMRA, Rox, TexasRed,

Cy3, Cy5

FAM, JOE, VIC, NED,

TAMRA, Rox, TexasRed, Cy3, Cy5

FAM, JOE, VIC, NED, TAMRA, Rox, TexasRed,

Cy3, Cy5

FAM, JOE, HEX, R6G, ROX,

TexasRed, Cy5, Cy5.5

Format 96-well plates 96-well plates rotor disk 96-well platesTest tube type (ml) 0.2 0.2 0.1 - 0.2 0.2Supported volumes (µl) 20-100 up to 50 10-50 10-100Weight (Kg) 64 21.4 14 27

Sacace thermalcycling protocols

UROGENITAL + CMV + EBV + TORCH + HPV INFECTIONS

Chlamydia trachomatis Real-TMNeisseria gonorrhoeae Real-TMTreponema pallidum Real-TMMycoplasma genitalium Real-TMUreaplasma parvum, Ureaplasma urealyticum Real-TMMycoplasma hominis Real-TMGardnerella vaginalis Real-TMHSV I,II Real-TMCMV Real-TM QualCMV Real-TM QuantEBV Real-TM QualEBV Real-TM QuantTrichomonas vaginalis Real-TMCandida albicans Real-TMChlamydia trachomatis / Ureaplasma spp. / Mycoplasma genitalium Real-TMChlamydia trachomatis / Ureaplasma spp. / Mycoplasma hominis Real-TMNeisseria gonorrhoeae / Chlamydia trachomatis / Mycoplasma genitalium Real-TMHSV I,II Typing Real-TMUreaplasma parvum / Ureaplasma urealyticum Real-TMTrichomonas vaginalis /Neisseria gonorrhoeae Real-TM

Chlamydia trachomatis / Ureaplasma spp. Real-TMChlamydia trachomatis / Mycoplasma genitalium Real-TMCandida albicans / Candida glabrata / Candida krusei Real-TMHSV / CMV Real-TMMycoplasma hominis / Gardnerella vaginalis Real-TMUreaplasma parvum / Ureaplasma urealyticum / Mycoplasma Real-TMTrichomonas vaginalis / Neisseria gonorrhoeae /Chlamydia trachomatis Real-TMNeisseria gonorrhoeae / Chlamydia trachomatis Real-TMPseudomonas aeruginos Real-TMPneumocystis jirovecii Real-TMCMV/EBV/HHV6 QuantHHV6 Real-TM QuantRubella Real-TMToxoplasma Real-TMParvovirus B19 Real-TM QuantHPV High Risk Screening Real-TM QuantHPV High Risk Typing Real-TMHPV 6/11 Real-TMHPV 16/18 Real-TM

38 different kits

Sacace thermalcycling protocols

NEUROLOGICALPoliovirus Real-TMNHS Meningitidis (N. meningitidis, H. influenzae, Str. pneumoniae) Real-TM

INTESTINAL INFECTIONSNorovirus/Astrovirus/Rotavirus Real-TMShigella/Salmonella/Campylobacter Real-TMEHEC Real-TM

Helicobacter pylori Real-TM

RESPIRATORY INFECTIONSInfluenza A/B Real-TMInfluenza A Real-TMInfluenza A H5N1 Real-TMInfluenza A H1N1 Real-TMInfluenza A H1N1/H3N2 Real-TM

Influenza A H5/H7/H9 Real-TMhRSV Real-TM

MTB INFECTIONMTB Real-TMMTB Diff Real-TM

2 different kits

2 different kits

7 different kits

4 different kits

Thanks for your attention