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1 Gerald Gale Wagner May, 2012 Name: Gerald Gale Wagner Title: Professor Office: Dept. Veterinary Pathobiology Phone: (409) 845-4275 College of Veterinary Medicine Fax: (409) 862-1147 & Biomedical Sciences Email: [email protected] Texas A&M University College Station, TX 77843-4467 Education: B.S. Texas Technological College, Lubbock, Bacteriology, 1963 M.A. University of Kansas, Lawrence, Microbiology, 1966 Thesis: Some aspects of the antibody response of guinea pigs to Coxiella burnetti. Ph.D. University of Kansas, Lawrence, Immunochemistry, 1968 Dissertation: Studies of rabbit immunoglobulins: Functional, chemical and physical properties of antibodies to bacteriophage ΦX174 Professional Experience and Academic Appointments: Postdoctoral Research Associate National Science Foundation 1968-70 Plum Island Animal Disease Center Greenport, New York Microbiologist Immunological Investigations 1970-71 Plum Island Animal Disease Center Greenport, New York Microbiologist and USDA Cooperative Research Division 1971-77 Official-in-Charge East African Veterinary Research Organization, Muguga, Kenya Associate Professor Department of Veterinary 1977-86 Microbiology and Parasitology Professor Department of Veterinary 1986-2012 Microbiology and Parasitology Interim Assistant Department Head Department of Veterinary 1990-91 Microbiology and Parasitology Coordinator of International Programs College of Veterinary Medicine and Bioimedical Sciences 1991-2011 Membership in Professional Organizations: American Association of Immunologists (emeritus) American Society for Microbiology (current) United States Animal Health Association (emeritus) Awards, Honors and Special Recognition: Elected to the Society of Sigma Xi 1971 Elected to the American Association of Immunologists 1973 Selected for inclusion in American Men and Women of Science 1977 Selected for inclusion in Who's Who in Frontier Science and Technology 1979 Texas Agricultural Experiment Station Team Research Award 1984

Gerald Gale Wagner Title Phone Fax Email - Texas A&M .... Ruwe, M.Sc., 1986, Biological Technician, Southwestern Medical School, Dallas, Texas I. Baradji, M.Sc., 1986, Research Microbiologist,

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Gerald Gale Wagner May, 2012

Name: Gerald Gale Wagner Title: Professor Office: Dept. Veterinary Pathobiology Phone: (409) 845-4275 College of Veterinary Medicine Fax: (409) 862-1147 & Biomedical Sciences Email: [email protected] Texas A&M University College Station, TX 77843-4467

Education: B.S. Texas Technological College, Lubbock, Bacteriology, 1963 M.A. University of Kansas, Lawrence, Microbiology, 1966 Thesis: Some aspects of the antibody response of guinea pigs to Coxiella burnetti. Ph.D. University of Kansas, Lawrence, Immunochemistry, 1968 Dissertation: Studies of rabbit immunoglobulins: Functional, chemical and physical properties of antibodies to bacteriophage ΦX174 Professional Experience and Academic Appointments: Postdoctoral Research Associate National Science Foundation 1968-70 Plum Island Animal Disease Center Greenport, New York Microbiologist Immunological Investigations 1970-71 Plum Island Animal Disease Center Greenport, New York Microbiologist and USDA Cooperative Research Division 1971-77 Official-in-Charge East African Veterinary Research Organization, Muguga, Kenya Associate Professor Department of Veterinary 1977-86 Microbiology and Parasitology Professor Department of Veterinary 1986-2012 Microbiology and Parasitology Interim Assistant Department Head Department of Veterinary 1990-91 Microbiology and Parasitology Coordinator of International Programs College of Veterinary Medicine and Bioimedical Sciences 1991-2011 Membership in Professional Organizations: American Association of Immunologists (emeritus) American Society for Microbiology (current) United States Animal Health Association (emeritus) Awards, Honors and Special Recognition: Elected to the Society of Sigma Xi 1971 Elected to the American Association of Immunologists 1973 Selected for inclusion in American Men and Women of Science 1977 Selected for inclusion in Who's Who in Frontier Science and Technology 1979 Texas Agricultural Experiment Station Team Research Award 1984

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Awards, Honors and Special Recognition (continued): Selected for inclusion in Who's Who in the South and Southwest 1985 Elected to Phi Beta Delta International Society 1990 Selected for inclusion in Who's Who in American Education 1995 Outstanding Faculty Award, TAMU Graduate Student Council 1997 Vice Chancellor’s Award in Excellence for International Involvement 1998 Nominated for the Bridges Chair in Veterinary Medical Education (declined) 2001 Named a USDA-APHIS Foreign Animal and Poultry Disease Expert 2001 Invited by the WHO to participate in the Malaria Genome Sequencing Consortium (declined) 2001 Selected for inclusion in Who’s Who in Medicine and Healthcare 2002 Invited to address an international symposium on tick-borne diseases in Obihiro, Japan 2002 Invited to address an international symposium on tick-borne diseases in Chonju, Korea (declined) 2002 Inducted into the Mexican Veterinary Academy 2002 Selected for inclusion in Who’s Who in Veterinary Medicine Higher Education 2003 Texas A&M University, Bush Excellence Award for Faculty in International Teaching 2008 Inducted as the first foreign member of the Royal Society for Veterinary Medical Science, Madrid, Spain 2009 Academic Committee Experience: Deputy Chancellor - Task Force for International Programs - 1989 Deputy Chancellor - Faculty Forum - 1984-1986 University - Graduate Faculty - 1977-present University - Graduate Diversity Fellowship Committee - 2005 University - Tropical Studies Committee - 1980-1990 University - Women in Development Steering Committee - 1991-1993 University - International Advisory Council - 1983-1984 University - International Programs Enhancement and Coordination Committee,1991-2011:

Activities: Chair, Subcmte on International Awareness,1992-94; Chair, Subcmte on International Student Issues, 1995-99; Member, Subcmte on International Memoranda of Agreement, 1995-96; Member, Subcmte on International Center for Bush Presidential Library,1995-96; Member, Wrkgrp on Reciprocal Exchange, MOA-related and other Special Program Students, 1999-2000; Member, Study Abroad Program and Policy Cmte, 2000-2011; Member Subcmte on International Undergraduate Research, 2005; Chair, Subcmte on International Memoranda of Agreement, 2006; Reviewer for Study Abroad Scholarship Applications, 2007-2011; Judge, Cultural Exhibits during International Week, 2008-09.

University - International Studies Degree Program, Executive Committee - 1993-2000 University - Provost’s International Affairs Degree Committee for the George Bush School of Government and Public Affairs - 1998-1999 University - L.T. Jordan Institute for International Awareness, Fellows Program Selection Committee - 1998-2002 University - European Union Center Management Committee – 2004-2008 University - Qatar Research Advisory Committee – 2004-2008 University - International Faculty Network Committee - 2004-2008 University - Fullbright Program Advisory Committee - 2004-2009 University – Association of Former Students, Graduate Merit Fellowship Program Selection Committee - 2007- 2009

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Academic Committee Experience (continued): University – International Curriculum Development Program, Review Committee – 2007-08. University – Member, Search Committee for the Dean of the College of Veterinary Medicine and Biomedical Sciences, 2008 University – Member, Search Committee for the Director of the TAMU, Mexico City Center, 2008 University – Bush Excellence Award for Faculty in International Teaching Selection Committee - 2008 University – Member, Selection Committee for the Association of Former Students, Distinguished Achievement Awards, 2009 University – Member, University Advisory Committee for the TAMU University Center in Mexico, 2009-2011 College - CVMBS Coordinator of International Programs – 1991-2011 College - CVMBS College Council - 1991-1994 College - CVMBS Scholarships and Convocations Committee - 1982-1986 College - CVMBS Graduate Instruction Committee - 1987-1990 and 1994-2002 College - CVMBS member on the Agricultural Program Faculty Advisory Committee - 1998-2002 College - COALS, Signature Program, Faculty Search Committee - 2004-2005 College - Faculty Search Committee - numerous Department - Curriculum Committee – 1980 Department - Representative to the Steering Committee for the Graduate Faculty in Microbiology-1984 Department - Graduate Advisor - 1984-1991, 1995-2001 Department - Graduate Advisory Committee - 1984-2001 Department - Promotion and Tenure Committee - 1986-2012 Department - Merger Committee - 1990-1991 Department - Review of Graduate Immunology Courses Committee - 1994 Department - Faculty Search Committee - numerous Activities with Students: Departmental Graduate Advisor - 1984-1991, 1995-2001 Major advisor for 22 PhD and 8 MSc candidates (see below), and member of the advisory committees for 32 PhD and 27 MSc candidates Faculty Advisor for the International Veterinary Student Association – 1997-2007 Program Director and advisor for the Global Veterinary Leadership Program – 2000-2009 Chair, Intern Selection Committee, TAMU, L.T. Jordan Institute for International Awareness, 1998-2011 Member, TAMU Fullbright Program Advisory Committee, 2004-2011 Member, TAMU Study Abroad Scholarship committee, 2005-2010 Member, TAMU Association of Former Students, Graduate Merit Fellowship Advisory Committee, 2006-2010 Mentor for TAMU Mexican Graduate Student Association – 1997-2001

Mentor for undergraduate students in the Howard Hughes Medical Institute Program, 1995-2000; Bridging the Baccalaureate Program, 1996-1998; Summer Research Scholars Program, 1996-1998; NASA-SHARP Program, 1999, 2001; Student Chapter, Mexican American Engineers and Scientists, 1999; Veterinary Apprenticeship Program, 1996-2000.

Occasional advisor for the CVM Graduate Student Association, 1995-2005, and the International Veterinary Student Association, 2005-2008 Visiting member of the Graduate Faculties of: University of Nairobi, Nairobi, Kenya, 1971-1977; University of Birmingham, England, 1973-1974; Colorado State University, Ft. Collins, 1973- 1975; University of the Witwatersrand, Johannesburg, South Africa, 1995-1997; University of Nuevo Leon, Mexico, 1995-1997; National Autonomous University of Mexico, 1991-present

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Graduate Students (as Major Advisor) and their current positions L.W. Johnson, M.Sc., 1978, Microbiologist, USDA, ARS, Pullman, Washington W. Goff, Ph.D., 1980, Research Leader, USDA, ARS, Pullman, Washington G.E. Elissalde, Ph.D., 1980, Resides in College Station, Texas D. Miller, M.Sc., 1982, Visiting Professor, Universidad Autonoma de Nuevo Leon, Mexico R. Long, M.Sc., 1982, Microbiologist, Brazos County Health Department. Deceased 1994 R. Teclaw, Ph.D., 1984, Epidemiologist, USDA, APHIS, Indianapolis, Indiana S. Sharp, Ph.D., 1985, Chief, Immunology Service, Miami General Hospital, Miami, Florida R. Nordgren, Ph.D., 1985, Head, Animal Vaccines, Merial, Kansas City, Missouri K. Ruwe, M.Sc., 1986, Biological Technician, Southwestern Medical School, Dallas, Texas I. Baradji, M.Sc., 1986, Research Microbiologist, Government of Mali C. Tripp, Ph.D., 1987, Research Leader, Heska Pharmaceuticals, Ft. Collins, Colorado B. Mamer, Ph.D., 1988, Diagnostician, Idaho Vet. Med. Diagnostic Lab., Caldwell, Idaho G. Carranza, M.Sc., 1986, Administrator, International Programs, TAMU G. Carranza, Ph.D., 1989, Associate Provost, North Texas State University K. Waldrup, Ph.D., 1991, Field Veterinarian, Texas Animal Health Commission A. Sahagun, M.Sc., 1992, Research Technician, Texas A&M University A. Sahagun, Ph.D. 1994, Professor, National Autonomous University of Mexico P. Holman, Ph.D., 1994, Research Scientist, Texas A&M University R. Droleskey, M. Sc., 1981, Research Technician, Texas A&M University R. Droleskey, Ph.D., 1996, Research Microbiologist, USDA, ARS, College Station, TX R. Makram, Ph.D., 1996, Research Microbiologist, Government of Egypt M. Hsieh, Ph.D., 1999, Post Doctoral Research Associate, University of Washington, Seattle R. Rosario Cruz, Ph.D., 1999, Research Microbiologist, INIFAP, Government of Mexico R. Hernandez, Ph.D., 1999, Research Microbiologist, INIFAP, Government of Mexico K. Genovese, Ph. D. (Co-chair), 1999, Research Microbiologist, USDA, ARS, College Station, TX M. Villarino, Ph.D., 2000, Livestock Production Consultant, Texas J. Steiner, Ph.D. (Co-chair), 2000, Associate Professor, Texas A&M University A. Tamayo, Ph.D. 2002, Assistant Professor, University of Baja California, Mexico M. Farnell, Ph.D. (Co-chair), 2003, Research Microbiologist, USDA, ARS, College Station, TX J. Suchodolski, Ph.D. (Co-chair), 2005, Clinical Assistant Professor, Texas A&M University As PhD or MS committee member: Graduate committees for 35 PhD and 32 MS students. Faculty advisor for international PhD student externs: Dr. Nasser Hegazy, Serum and Vaccine Research Institute, Cairo, Egypt, 1993-94 Dr. Ensaf Khashabah, Serum and Vaccine Research Institute, Cairo, Egypt, 1993-94 Dr. Romany Makram, Serum and Vaccine Research Institute, Cairo, Egypt, 1994-95 (completed a PhD program at TAMU in 1996) Dr. Rashid Ahmed, Livestock Production Research Institute, Bhadurnagar, Pakistan, 1997 Dr. Yazmin Alcala, National Autonomous University of Mexico, Mexico City, Mexico, 1999-2000

Editorial Service (last 10 years): Member of the Editorial Review Committee for Veterinaria Mexico, a quarterly research-oriented, peer reviewed journal published by the National Autonomous University of Mexico, 2000 - 2011. Manuscripts reviewed for Veterinary Immunology and Immunopathology, Infection and Immunity, Vector-Borne and Zoonotic Diseases, Veterinary Parasitology, Veterinary Microbiology, Experimental

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Editorial Service (last 10 years) (continued): Parasitology, American Journal of Veterinary Research, Zoonoses and Public Health, and the Journal of Medical Entomology. Grant Review Committees (last 10 years): Grant proposals reviewed for USDA-NRI; USDA-APHIS; USDA-ARS; the International Foundation for Science (Sweden), the CONACyT (Mexico), the InterAmerican Development Bank (Mexico), and several bilateral programs administered by the US Dept. of State. National and International Committees, Boards, Consultancies and Review Teams: Research Advisor, International Centre of Insect Physiology and Ecology, Nairobi, Kenya, 1972-1977. Technical Advisor, U.S. Agency for International Development (USAID) - animal health projects in Kenya, Ethiopia, Uganda, Tanzania and Mali, 1971-1981, 2006. Consultant to IMC - Hemoparasitic disease diagnostic test development. 1979-1980. Consultant to Beecham Laboratories - Hemoparasitic disease diagnostic test development. 1979-1980. Consultant to Pfizer - Hemoparasitic disease chemotherapy. 1980-1984; 1986-1988; 1990. Technical Advisor, Interamerican Institute for Cooperation in Agriculture, animal health projects in Mexico, 1980-1984. Member, National Research Council, Board on Agriculture, Subcommittee on the Long-range Plan for Foreign Animal Disease and Ectoparasite Diagnosis and Research, 1982-1984. Member of the Emergency Animal Disease Task Force, Texas Veterinary Medical Diagnostic Laboratory System, 1983-2008. Consultant and Review Team Leader, Review of the Trypanosomiasis Research Project at the International Laboratory for Research on Animal Diseases (ILRAD), United Nations Development Programme, Nairobi, Kenya, 1984. Member, U.S. Animal Health Association (USAHA) 1988 -2011; Chair of the Foreign and Emerging Disease Committee, 1993-1998; Member of the Annual Program Committee, 1997-2000; Chair of

the Parasitic Diseases Committee, 1999-2003. Appointed by the President, Texas A&M University, to review animal health research and possible collaborative research programs with Makerere University, Kampala, Uganda, 1990. Consultant to USDA/APHIS/VS for Foreign Animal Disease Awareness courses, 1991-1996. Consultant to Becton Dickinson, Tropical Diseases Section, for development of diagnostic tests for hemoparasitic diseases 1991-1992. Consultant to Mattek Corporation - Hemoparasitic disease diagnostic test development.1991-1995. Member, External Academic Advisory Council of the Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, Mexico, D.F., 1992-1995, 2006-2010. Project Director, USAID University Development Linkage Program in Mexico, 1992-1999. Member, Internal Academic Advisory Council of the Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, Mexico, D.F., 1994-1997. Member, USDA Review Panel, Foreign Animal Disease Diagnostic Capability, Plum Island, 1995. Member, International Affairs Committee, Association of American Veterinary Medical Colleges (AAVMC), 1995-2005. Member, Institute for Food Safety and Education, Center for Food Safety, Texas A&M University 1995-2010. Member, USDA Review Panel, Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX, 1997, 2007. Member, the Dean’s Panel, Society of Mexican American Engineers & Scientists, 1998. Consultant, Blue Ridge Pharmaceutical Company, hemoparasitic disease chemoprophylaxis. 1998. Member, Texas Emergency Response Team, a workgroup organized by the Texas Animal Health Commission, 1998-2000.

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National and International Committees, Boards, Consultancies and Review Teams (continued): Organized the “Global Veterinary Leadership Program” for new veterinary students, Queretaro, Mexico, July, 2000. Consultant to USDA/APHIS/VS for Equine Piroplasmosis regulatory programs, 2003, 2005, 2010 External Referee, Promotion & Tenure Committee, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, 2004 Consultant to USDA/APHIS/VS for Tick and Tick-borne Disease regulatory programs, 2001, 2005-08 Member, American Councils for International Education, US Dept. Education Grant Advisory Committee, 2006-2007 Member, USDA Selection Panel for candidates for the position of Director, USDA Livestock Insects Research Laboratory, Kerrville, TX, 2008. Consultant to the United Nations Development Programme (UNDP) for design of a multi-year project to strengthen SAGARPA (the equivalent to APHIS) in Mexico,.2008-2009. Member, Search Committee for the Director for Mexico and Latin American Programs, Texas A&M University, 2009. Member, University Advisory Committee for the Texas A&M University Center in Mexico, 2009- 2012. Member, Review Committee for the Scholarship Program, University Association of Former Students, 2008 - 2010 Ad hoc consultant, to the USDA Livestock Insect Research Laboratory, Kerrville, TX, 2008 –2012. Request from Drs. Marguerite Pappaioanou, CDC, and Alfonso Torres, Dean, College of Veterinary Medicine, Cornell University, to re-write and update the concept paper entitled “Global Veterinary Leadership” for use as a ‘concept paper’ for internal training activities at the CDC. 2011. . Participation in various organizations and activities: Co-chair, Symposium on the Bovine Immune System, College Park, Maryland, 1970. FAO/OIE Conference on Protozoal Tick-borne Diseases of Cattle, Nairobi, Kenya, 1972. Session Chair, 3rd International Congress of Parasitology, Munich, Germany, 1974. WHO Conference - Immunology of Infectious Diseases, Lausanne, Switzerland, 1974. Joint WHO/FAO Program - Immunology of East Coast Fever, Nairobi, Kenya, 1974. Joint USAID/BIFAD Workshop on the Title XII Animal Health Collaborative Research Support Program, Washington, D.C., 1980. Co-organizer (with USDA, ARS) of the series of joint U.S./Mexico Tick Technical Meetings, in Texas (1979, 1980, 1986, 1994 and 1997) and in Mexico (1982, 1984, 1995 and 1997). Presented "Tick-borne Diseases of Cattle" to the National Cattlemen's Association, Research and Education Committee meeting, College Station, 1981. Presented "Tick-borne Diseases of Cattle" to the Annual Meeting of the Mexican Association of Veterinary Parasitologists, Villahermosa, Tabasco, 1981. Session Chair, 7th National Anaplasmosis Conference, Mississippi State University, 1981. Organizing Committee and Session Chair, 4th International Conference of the Association of Institutes of Tropical Veterinary Medicine, Orlando, 1983. Organizing Committee and Session Chair, International Symposium on Hemoparasitic Diseases, College Station, 1983. Organizing Committee, Fall Meeting, Texas Branch of the American Society for Microbiology, College Station, 1983. Organizing Committee and Session Chair, Symposium on Immunoregulation of Parasitic Diseases, College Station, 1983. Session Co-chair, 58th Annual Meeting of the American Society of Parasitologists, San Antonio, 1983.

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Participation in various organizations and activities (continued): Organizing Committee, 2nd International Conference of Effector Mechanisms in Domestic Animals, College Station, 1985. Organizing Committee and Session Co-chair, 40th Annual Meeting of Animal Disease Research Workers in the Southern States, College Station, 1987. Session Co-chair, 14th International Symposium on Controlled Release of Bioactive Materials, Toronto, Canada, 1987. Joint USDA/ARS and APHIS Meeting on Cattle Fever Tick - Technical Orientation and Research Priorities, McAllen, Texas, 1987. Co-organized the conference: "Mexico-USA: Past, Present and Future", Texas A&M University, 1987. Reviewed the development of recombinant protozoal and helminthic vaccines for Salisbury Laboratories, Inc., Charles City , Iowa, 1988. Presented "DNA Probes for Babesiosis," to the plenary session, 85th Annual Meeting of the USAHA, St.Louis, 1989. Co-organized the International Conference on Bacterial Diseases in Cattle, Monterrey, Mexico, 1989. Session Chair, 8th National Veterinary Hemoparasite Disease Conference, St. Louis, 1989. Presented "Tick-borne Diseases," to the Regulatory Veterinary Medicine Section, at the 127th Annual Meeting of the AVMA, San Antonio, 1990. Presented "Tick-borne Disease and Trade," to the Tropical Diseases Section, at the International Stockman's School, Houston, 1991. Presented "Tick-borne Disease and Trade," to the Texas Consortium on Free Trade Conference, sponsored by the Texas Department of Commerce, Austin, 1991. Member of the faculty for the annual USDA/APHIS Foreign Animal Disease Courses, held in various locations in the U.S. 1991-1996. Co-organized the Tri-county Beef Cattle Herd Health Program, Matthews Ranch, Albany, Texas, 1991. Co-organized the Inaugural, 2nd and 3rd Annual "Technology for Latin America" conferences, College Station, 1992, 1993 and 1994. Co-organized the 9th International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993. Participated in the First Congress of the PanAmerican Federation of Colleges of Veterinary Medicine, Mexico City, Mexico, 1993. Presented "International Education and Research Agreements," at the 6th Annual Mexico-Texas Higher Education Conference, TAMU-Kingsville, 1994. Organized the Mid-Term Review of the USAID University Development Linkage Program for Mexico, meetings in Mexico City and in College Station, 1995. Presented "Funding for Collaborative Programs with Mexican Institutions" at the TAMU Faculty Development Seminar, Mexico City, 1995. Participated in the conference "Strategic Support Systems for Cattle Ranching in 2000," organized by the Office of Mexico and Latin American Programs, TAMU, 1995. Participated in the conference “The Farm Bill and Beyond” organized by the Texas Agricultural and Natural Resource Summit Initiative, Lubbock, Texas, 1996. Presented "Equine Babesiosis," to the International Andalusian & Lusitano Horse Association, Las Vegas, NE 1996. Presented "Collaborative International Research Programs," to the Committee on International Affairs, AAVMC, Louisville, 1996. Participated in the 14th Symposium on Veterinary Medical Education, Athens, Georgia, 1996. Panel member: "Regionalization under GATT and NAFTA, the changing world order." Texas Veterinary Medical Association Summer Seminar, Austin, 1997.

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Participation in various organizations and activities (continued): Presented "Animal and Plant Health Problems Important to Free Trade between Mexico and the U.S.," at the USAID Global Bureau Conference on Human Capacity Development for the 21st Century, Washington, D.C., 1997. Participated in the USDA Program Review, Livestock Insects Research Laboratory, Kerrville, 1997. Participated in the Legislative Strategy Meeting, USAHA, Washington, D.C., 1998. Participated in the Risk Assessment Working Group, USDA, APHIS, VS, Washington, D.C. 1998. Presented "Animal Disease Research in Mexico," to the Kenedy County Commissioners Court, Sarita, Texas, 1998. Participated in the Annual Project Director’s Meeting, US Department of Education, International Mobility in Higher Education Program, Huntsville, Canada, 1998. Presented "Establishing Collaborative International Veterinary Research Programs," at the USDA Regional Workshop on Globalizing Agricultural Science and Education Programs for America, Houston, 1998. Presented "Non-Pathogenic Theilerias," to the faculty of the WHO Collaborating Center for Tropical Diseases, UTMB, Galveston, 1998 Participated in the inaugural meeting of the Gulf Coast Region Tropical Medicine Club, Galveston, 1998. Presented "International Cooperative Agreements," at the TAMU/COALS International Agricultural Programs Retreat, College Station, 1998. Reviewed animal agriculture programs for the Livestock Association of Tabasco, Villahermosa, Mexico, 1998. Presented "Establishing Collaborative International Veterinary Research Programs," at the USAID Higher Education Partnership in Development Conference for Latin America and the Caribbean. Tegucigalpa, Honduras, 1999. Participated as Chair, Working Group on Establishing Collaborative International Veterinary Research Programs, at the USDA Workshop on Mobilizing the Veterinary Profession to Recognize and Respond to Exotic animal Disease Threats, Ames, 1999. Participated in the conference: “Reconstruction and Transformation of Central America - A Roundtable Discussion with Policymakers,” College Station, 1999. Participated in the Regional Fund for Agricultural Technology (FONTAGRO) workshop. College Station, 1999. Participated in the Annual Project Director’s Meeting, US Department of Education, International Mobility in Higher Education Program, Mexico City, 1999. Presented "Veterinary Curricula in the Next Millennium - Emerging Diseases, Food Safety and Food Security," to the plenary session, AAVMC, Washington, D.C. 1999. Participated in the IX International Symposium of Veterinary Laboratory Diagnosticians/OIE Seminar on Biotechnology. College Station, 1999. Participated in the 2nd Biennial Texas Conference on International Education, Texas Higher Education Coordinating Board, Austin, 1999. Presented "Establishing Collaborative International Veterinary Research Programs," at the USDA, International Services Division conferences on “Planning for Animal Health Surveillance” in Managua, Nicaragua, and in Tegucigalpa, Honduras, 2000. Participated in the Annual Project Director’s Meeting, US Department of Education, International Mobility in Higher Education Program, Austin, 2000. Presented "Diseases of Exotic Animals in Transit," at the International Millennium Meeting of the Animal Transportation Association, Houston, 2000. Participated in the Annual Project Director’s Meeting, US Department of Education, International Mobility in Higher Education Program, San Diego, 2000. Presented "Veterinary Curriculum in the Next Millennium - Emerging Diseases, Food Safety and Food Security" at the College of Veterinary Medicine, Universidad Austral de Chile, Valdivia, 2000.

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Participation in various organizations and activities (continued): Presented a briefing on "Ticks and Tick-borne Diseases in Texas and Mexico," at the invitation of the Commissioner of Agriculture, State of Texas, Austin, 2000. Presented a briefing on "International Animal Health," at a meeting for U.S. Agricultural Attaches, during the National Food & Beverage Marketing Conference, Miami, 2000. Organized and participated in the international conference "Preparing the Veterinary Profession for Corporate and Trade Issues in the Americas," Santiago, Chile, 2001. Participated in a panel discussion, “Increasing the International Capacity of Higher Education," at the Association of International Education Administrators meeting, Tucson, 2001. Participated in a panel discussion, "Partnerships and Sector Impacts," USAID meeting, Bethesda, 2001. Participated in the Annual Project Director’s Meeting, US Department of Education, US/Brazil Higher Education Consortia Program, Austin, 2001. Member of the invited faculty to the annual USDA/APHIS Foreign Animal Disease Course, held at Plum Island Animal Disease Center, New York, 2001. Participated in the Annual Project Director’s Meeting, US Department of Education, International Mobility in Higher Education Program, San Diego, 2001. Presented "Bioterrorism: An International Challenge to Animal Agriculture" at the National Institute for Animal Agriculture (NIAA) meeting, Colorado Springs, 2001. Participated in a panel discussion, "Tick-borne Diseases," USDA APHIS Workshop on Acaricide Resistance in Cattle Fever Ticks in Mexico, Kerrville, 2002 Presented "U.S.-Mexico Free Trade - Animal Health Issues," at the 8th Agricultural and Food Policy Information Workshop, Puerto Vallarta, Mexico, 2002. Participated in the USDA APHIS Workshop on Anaplasmosis - Science and Policy, Fort Collins, 2002. Presented "Ticks and Tick-borne Diseases," to the Cattle Health and Well-being Committee, National Cattlemen's Beef Association (NCBA) summer conference, Reno, 2002. Organized the symposium, "Global Veterinary Medical Education" at the annual AAVMC meeting, Washington, 2002. Organized and participated in the "Symposium on International Education," at the annual meeting of the American Veterinary Medical Association (AVMA), Denver, 2003. Working group moderator at the Global Veterinary Opportunities and Responsibilities Workshop, jointly sponsored by the AAVMC, USDA and the US Army Veterinary Corps, San Antonio, 2004. Participated in a panel discussion, "Risk Management in the Integrated NAFTA Market: Lessons from the Case of BSE,." at the inaugural Annual North American Agrifood Market Integration Workshop, Cancun, Mexico, 2004 Organizing Committee, 2nd Annual North American Agrifood Market Integration Workshop. San Antonio, 2005. Participated in the Department of Homeland Security (DHS), Center for Foreign and Zoonotic Diseases (FAZD) semi-annual meetings in Davis, CA, and in College Station, 2005. Presented "Evaluating tick and tick-borne diseases as threats to the Gulf Coast and Southeast U.S.," to the Cattle Health and Well-being Committee, NCBA annual meeting, San Antonio, 2005. Presented "Digital Governance for Animal Health and Biosecurity Applications," at the National Science Foundation, Annual Conference for Digital Government Research, Atlanta, 2005. Organizing Committee, Roundtable on Risk Analysis and Animal Health Policy, North American Agrifood Market Integration, College Station, 2005. Participated in the design of an international, scenario-driven, FMD simulation, for a conference on international law, Case Western Reserve University School of Law, Cleveland, 2006 Presented "Texas fever and cattle fever ticks," to the Cattle Health and Well-being Committee, NCBA annual meeting, Denver, 2006. Participated in the Department of Homeland Security (DHS), Center for Foreign and Zoonotic Diseases (FAZD) semi-annual meetings, alternately in Davis, CA and in College Station, TX, 2005-07.

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Participation in various organizations and activities (continued): Organized and participated in a series of international conferences "US-Mexico Cattle Fever Tick Round Table Forum," in Mexico City, Fall, 2006, in Piedras Negras , Spring, 2007; in Kingsville, Texas, Fall, 2007, and in Mexico City, Spring, 2008. . Presented "Science, Politics and Texas Cattle Fever" at the annual Texas Agricultural Experiment Station conference, in College Station, 2007. Presented "Science, Politics and Texas Cattle Fever" at the international conference "US Cattle Fever Tick Eradication Program: 100 years of protecting the US cattle industry from Texas Cattle Fever," in Kingsville, 2007. Presented "Science, Politics and Texas Cattle Fever" by video conference to faculty at the College of Medicine, East Tennessee State University, Johnson City, 2007. Presented “An update on Texas Cattle Fever (Babesiosis) and Cattle Fever Ticks (Boophilus microplus and B. annulatus). Ticks and Tick-borne Diseases Committee, Annual meeting of the U.S. Animal Health Association, Reno, NV, 2007. Presented “Globalization of Veterinary Medical Education” to faculty and students at the Faculty of Veterinary Medicine, University of Padua, Padua, Italy, 2007. Presented "Science, Politics and Texas Cattle Fever" to the International Veterinary Students Association, Texas A&M University, January, 2008. Presented “The Threat of Transatlantic and Global Pandemics” at the EU Center workshop: “The European Union: Addressing Global Issues,” College Station, January, 2008. Participated in an ad hoc meeting on Cattle Fever Ticks, Texas Animal Health Commission, Laredo, TX, March, 2008. Participated in an ad hoc meeting on the Social Context of Foot and Mouth Disease Control – survey Design workshop, College Station, TX, Cattle Fever Ticks, Texas Animal Health Commission, Laredo, TX, March, 2008. Participated in an ad hoc meeting on the concept of Global Veterinary Leadership, with Drs. Marguerite Pappaioanou (CDC) and Dr. Alfonso Torres, Dean, College of Veterinary Medicine, Cornell University, concerning the Global Veterinary Leadership Program. College Station, April, 2010. International Training Activities: Organized workshops, seminars and training for visiting scientists and veterinarians, involving the Dean and two to eight faculty, in collaboration with the College of Agriculture and Life Sciences, Office of International Agriculture Programs, the Texas Veterinary Diagnostic Lab, and/or the TAMU International Programs Office. 1991 - Workshop on hemoparasitic diseases of livestock - veterinarians from the Livestock Producer’s Association, Monterrey, Nuevo Leon, Mexico. 1992 - Workshop on babesia cell culture techniques - scientists from the Onderstepoort Research Institute, Pretoria, South Africa. 1993 - Workshop on babesia cell culture techniques - scientists from the Onderstepoort Research Institute, Pretoria, South Africa. 1993-94 - Training on recombinant hemoparasite vaccines - two scientists from the Serum and Vaccine Research Institute, Cairo Egypt. 1995-97 - Training on babesia cell culture and recombinant hemoparasite vaccines - scientist from the Veterinary Research Institute, Cairo, Egypt. 1995 - Workshop on hemoparasitic diseases of livestock - scientist from the Diagnostic Section, AgCanada Food Production and Inspection Branch, Nepean, Ontario. 1995 - Workshop on babesia cell culture - laboratory technicians from the Colleges of Veterinary Medicine, National Autonomous University of Mexico, and the Autonomous University of Yucatan.

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International Training Activities (continued): 1995 - Workshop on higher education curriculum and teaching methods - government and industry officials, and university administrators from Indonesia. 1996 - Workshop on hemoparasitic diseases of livestock - professor from the Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. 1996 - Workshop on hemoparasitic diseases of livestock - professor from the Haryana Agricultural University, Haryana, India. 1996 - Workshop on advanced babesia cell culture techniques - laboratory technician from the College of Veterinary Medicine, National Autonomous University of Mexico, Mexico City 1997 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Tunisia. 1997 - Workshop on hemoparasitic diseases of livestock - scientist from the Livestock Production Research Institute, Bhadurnagar, Pakistan. 1997 - Workshop on veterinary medicine and international trade - regulatory veterinarians from the Czech Republic, the Slovak Republic, Hungary, Estonia, Lithuania, Croatia and Slovenia. 1998 - Workshop on veterinary medicine and food safety - government regulatory veterinarians from Poland, Yugoslavia, Romania, Bulgaria and Latvia. 1998 - Workshop on food regulation and inspection - government regulatory veterinarians from Brazil. 1998 - Workshop on veterinary medicine and food safety - government regulatory veterinarians from Kazakstan, Kyrgyzstan, Usbekistan, Turkmenistan and Tajikistan. 1998 - Workshop on veterinary medicine and international trade - government regulatory veterinarians, an industry official, and a professor of veterinary medicine from Poland. 1999 - Seminar on veterinary and agricultural biotechnology - university professors from Taiwan. 1999 - Seminar on veterinary and agricultural biotechnology - government official from Chile. 1999 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Poland. 1999 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Romania and Bulgaria. 1999 - Seminar on continuing education programs for veterinary medicine - officials from the Japanese Veterniary Medical Association. 1999 - Workshop on veterinary education curriculum and teaching methods - university officials from the ANGR Agricultural University of India. 2000 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from the Ukraine. 2001 - Veterinary education and biotechnology - FSIS class of foreign veterinarians involved in food safety. 2001 - Workshop on poultry, bovine and wildlife disease problems - government regulatory veterinarians from Poland. 2002 - Veterinary education and biotechnology - FSIS class of foreign veterinarians involved in food safety. 2002 - Veterinary education - officials from the Ministry of Education, Government of Spain. 2002 - Veterinary education - officials from the Ministry of Education, Government of Bolivia. 2002 - Veterinary education - officials from the Ministries of Agriculture and Education, Government of Ecuador. 2002 - Workshop on veterinary education, organization and administration – government regulatory veterinarians from Trinidad, Uganda and Panama. 2002 – Workshop on Global Veterinary Medicine and Biosecurity, Obihiro University, College of Veterinary Medicine, Obihiro, Japan 2003 - Veterinary education and biotechnology - FSIS class of foreign veterinarians involved in food safetty.

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International Training Activities (continued): 2003 - State-federal relationships and biosecurity - government regulatory veterinarians from Poland. 2004 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Eastern Europe. 2005 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Eastern Europe and Central Asia. 2006 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Eastern Europe and Central Asia. 2007 - Workshop on veterinary medicine and veterinary medical education – faculty members from the Veterinary College, China Agricultural University, Beijing. 2007 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Ethiopia. 2007 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Kosovo and Macedonia. 2007 - International veterinary medical programs – presentation to the Vice President, the Republic of Uganda, and his delegation. 2008 - Workshop on medicine and international trade - government regulatory veterinarians from Jordan, Ghana, Nigeria, Yemen and Tanzania. 2009 - International veterinary medical programs – presentation to the President of Baghdad University, and the President of Kirkuk University. 2009 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Moldova. 2009 – Veterinary education – officials from the Board of Regents, Sri Venketeswara Veterinary University, Andhra Pradesh, India 2010 - Workshop on veterinary medicine and international trade - government regulatory veterinarians from Kazakhstan

Other Activities: Member of the Board of Directors of the Twin City Mission, Bryan, Texas, 1986-1996, President of the Board of Directors, 1989-90. City of College Station, Texas, Committee for Comprehensive Development Plan, 1980-81, and Zoning Board of Adjustment, 1980-86. Reviewer, Annual Science Fair poster session, Texas A&M University, 2010-11.

Teaching Activities: Teaching interest and experience has been in unfergraduateimmunology courses, and in professional courses in under-emphasized areas such as emerging diseases, biosecurity, veterinary public health and food safety. International veterinary education has been a long term interest, particularly curriculum harmonization, and education in veterinary regulatory medicine, biosecurity, and free trade. Graduate education has been a very important activity, as has the international exchange of students and faculty. Formal instructional activities have included:

Instructor for VTMI 409 - Immunology (undergraduate) Author/instructor for VTMI 645 - Host-Agent Interactions in Veterinary Medicine (graduate)

Instructor for VTMI 649 - Immunology (graduate) Instructor for VTMI 650 - Experimental Immunology (graduate) Instructor for VTMI 941 - Senior Didactic Rotation (professional) - current Instructor for VPAR 605 – Immuno-parasitiology (graduate)

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Courses taught (last 5 years): Course Title Institution Credit Hr % of Course Dates Contact Hr Undergraduate: VTPB 409-Immunology TAMU 3 25 Spr 07, Fall 07 20 VTPB 409-Immunology TAMU 3 25 Spr 08, Fall 08 20 VTPB 409-Immunology TAMU 3 25 Spr 09, Fall 09 20 VTPB 409-Immunology TAMU 3 25 Spr 10, Fall 10 20 VTPB 409-Immunology TAMU 3 25 Spr 11, Fall 11 10 Courses taught (last 5 years) (continued): Professional: VTMI 941- Senior Didactic TAMU 3 5 02-2012 36 Rotation SEP 929 Biosecurity FSIS 3 5 02-03 12 Smith-Kilborne FADD training USDA (Cornell) 3 20 May 03, 04 5 Competitive Grant Support for Education: Title Source Funding Year Role Animal Hlth, Food Safety & Wildlife Dis DoEducation 179,000 97-00 PI Global Vet Leadership/Signature Prog TAMU-CVM 7,500 99-00 PI UG International Studies & Language DoEducation 198,000 99-01 CoI Vet Ed - Emerg Dis, BioT & Food Security DoDefense 388,802 00-04 PI Globalization of Vet Ed in the Americas DoEducation 107,019 02-06 PI FAD Digital Library for Dx, Conf & Ed USDA Challenge 99,999 00-02 PI Digital Governance NSF 55,000 04-06 PI Vet Ed – Veterinary Biosecurity DHS 85,000 05-10 CoI

Last 5 years: National Homeland Security Center on Foreign Animal and Zoonotic Diseases. Tammy Beckham, Director. CoPI with Morgan Scott (thru 2008) and Bo Norrby (thru 2009) on Veterinary Education. US Department of Homeland Security - ONR 14-04-1-0). Total funding $149,055 for 5 years, 2005-10.

Education Meeting Participation: Abstracts: 1. Animal health regulations and international trade. G. Wagner* Proceedings, Conference on Technology for Latin America, College Station, Texas, 1992. 2. Increasing the International Capacity of Veterinary Education. G. Wagner. Annual Meeting of the Association of International Education Administrators. Tucson, Arizona, February, 2001. 3. University Partnerships in Research and Applied Technology. Joint USAID/NIH Workshop on Human Capacity Development. Bethesda, Maryland, August 2001. 4. Managing Successful Consortia Programs.” G. Wagner. Department of Education, US-Brazil Higher Education Consortia Program, 2001 Bilateral Project Director’s Meeting, Austin, Texas, October 2001. 5. Developing International Curricula. G. Wagner. Department of Education, Fund for the Improvement of PostSecondary Education, 2001 Project Director’s Meeting, San Diego, California, November, 2001. 6. Canada, US and Mexico Cattle Issues. G. Wagner and R. Ochoa. Policy Disputes Information Consortium, 8th Agricultural and Food Policy Workshop, Puerto Vallarta, Mexico, March, 2002.

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Education Meeting Participation – Abstracts (continued): 7. The use of the Intercultural Development Inventory as an Assessement Tool for Determining Intercultural Competence - US-Brazil Veterinary Student Exchanges. C. Drennon, C. Brown, C. Barros, G. Wagner, I. Carbajal and R. Santos. Department of Education, US-Brazil Higher Education Consortia Program, 2003 Bilateral Project Director’s Meeting, Miami, FL October 2003. 8. Internationalization of the Curriculum at U.S. Veterinary Colleges. Invited presentation and panel discussant, NAFSA/Association of International Educators annual conference, Washington, D.C., May, 2008.

Refereed Proceedings: 1. Preparing the Veterinary Profession for Corporate and Trade Issues in the Americas: Proceedings of a conference on Synergism and Globalization, Santiago, Chile, May 6-8, 2001. C. Brown, I. Carbajal and G. Wagner. J. Vet. Med. Ed. 28:56-61 (2001). 2. A Digital Governance System for Animal Health and Biosecurity Applications. G. Wagner, A. Vedlitz and S. Waghela. Annual Project Director’s meeting, NSF Digital Governance Program. Atlanta, Georgia, May, 2005. 3. Overview of TAMU, CVMBS International Program. G. Wagner. Annual meeting of the American Veterinary Medical Association, International Programs Committee, Minneapolis, Minnesota, July, 2005.

Bibliography:: Educational Publications in Refereed Journals: 1. Educational preparedness of veterinarians for foreign animal diseases. M. Thurmond, P. Gibbs, C. Brown, G. Wagner, T. Wilson and B. Lautner. J. Amer. Vet. Med. Assoc. 222:1352-1357 (2003). 2. Texas A&M University's College of Veterinary Medicine and Biomedical Sciences: Global Veterinary Leadership Program. G. Wagner. J. Vet Med Educ, 33:299-300 (2006). Book Chapter: Global Veterinary Leadership. G. Wagner and C. Brown. In: Veterinary Clinics of North America, (Vet. Clin. Food Anim. Vol. 18); Ed. J. England. Elsevier Science, Philadelphia, 2002. 417-430. Non-refereed Publications/Interviews: 1. Veterinary students learn about bioterrorism in academic environment. In The Battalion, Aug., 2000. 2. AAVMC institutions unite to solve global problems. In Animal Matters, Nov. 2000. 3. Globe trotters - The global veterinary leadership program. In CVM Today, Winter, 2001 4. Corporate, trade issues unfold at Chile summit. In AVMA Onlinenews, July, 2001. 5. Colleges launch global veterinary programs. In DVM Magazine - Career Builder. Sept. 2001, pp. 6-7. Research Activities: Research training and experience has been directed toward inter-disciplinary studies of immune defense mechanisms in viral and hemoparasitic infections, especially effector mechanisms of resistance. Current projects support work on the molecular basis of virulence of protozoal parasites; identification of functional bacterial and viral antigens for serodiagnosis and immunization; and development of recombinant antibodies. Another major project involves the development of relational databases for the diagnosis of livestock diseases, and decision support systems to aid in the control of outbreaks of animal or zoonotic disease.

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Competitive Grant Support for Research Title Source Funding ($/year) Years Role Foreign Animal Disease Investigations USDA 9 ,000 71-77 PI Diagnosis of Hemotropic Diseases TAMU 2,000 77-78 CoI Ticks and Tick-borne Diseases of Cattle USDA 20,000 77-79 CoI Animal Health in Tropical Environment TAES 16,000 78-83 PI Efficacy of Experimental Drugs Pfizer 13,800 79-82 PI Bovine Brucellosis USDA 3,000 77-78 PI Canine Neutrophil Defects NIH 20,000 77-78 CoI Chronic Anaplasmosis in Cows TAMU 10,000 80-83 CoI Antigens of Brucella USDA 5,000 82-84 CoI Bovine Hemoparasitic Diseases USDA 12,000 78-82 PI Tick Ecology and Control TAES 13,000 79-82 CoI Tick-borne Diseases in Mexico USDA 22,000 83-85 PI Ticks and Tick-borne sses of Cattle TAES 12,000 83-85 PI Control of Fever Ticks and Texas Fever TAES 40,000 83-85 CoI Efficacy of Experimental Drugs Pfizer 3,900 86-87 PI Ticks and Tick-borne Diseases of Cattle TAES 15,000 86-88 PI Control of Fever Ticks and Texas Fever TAES 95,000 86-88 CoI Tick-borne Protozoan Diseases TAES 7,000 88-90 PI Control of Fever Ticks and Texas Fever TAES 23,350 88-90 CoI Bovine Babesia DNA Probes USDA 25,600 88-90 PI Control of Fever Ticks and Texas Fever TAES 30,000 90-92 CoI Bovine Anaplasmosis Vaccines USDA 21,000 90-92 PI Bovine Infectious Diseases TAES 10,600 91-93 CoI Equine Babesia DNA Probes USDA 29,305 90-94 PI Animal Health in Mexico USAID 100,000 92-97 PI Anaplasmosis vaccines Schering 33,000 92-93 CoI Recombinant hemoparasite vaccines ENARP 16,000 93-94 PI DNA probes for acaricide resistance USDA 35,000 93-97 PI Equine Babesia DNA Probes AHSA 2,500 95-96 PI Virulence factors for Babesia USDA 24,500 94-96 PI Mechanisms of Acaricide Resistance in Ticks USDA 35,000 95-98 PI Epidemiology of Tick Acaricide Resistance USDA 14,000 98-99 PI OP Resistance in Boophilus microplus ticks USDA 20,000 97-99 CoI Emerging Infectious Diseases of Trade TAES 7,000 99-04 PI Monoclonal Antibodies for Colibacillosis Texas ARP 54,000 98-00 CoI' Recombinant hemoparasite vaccines ENARP 12,000 99-01 PI Molecular Diagnosis of Babesia equi Texas ARP 46,000 00-02 CoI Internal Monitors of Health Status NASA/JPL 12,500 00-01 PI Mechanisms of Acaricide Resistance in Ticks USDA 25,000 99-02 PI Control of Avian Coccidiosis TAMU-CVM 15,000 00-02 PI Detection of B.microplus acaricide resistance USDA 17,000 01-03 CoI Ecological risk factors of disease in cattle USDA 25,000 01-03 CoI Epidemiology of Classical Swine Fever USDA 120,000 02-04 CoPI Detection of anthrax prior to clinical signs USDA 152,647 03-04 CoI

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Competitive Grant Support for Research - Last 7 years: A Digital Governance System for Animal Health and Biosecurity Applications. PI with Surya Waghela. NSF. Total funding $55,000, 2004-05. Rapid Pen Side Diagnostic Test for Classical Swine Fever. Co-investigator with Surya Waghela, and Austin Sequeira, Omnisite, Inc (PI) USDA-SBIR. Total funding $80,000. 2005-06. Preventing colibacillosis diarrhea in bovine calves with anti-F5 recombinant antibodies produced in rice plants. PI with Surya Waghela and Alfredo Sahagun. CONACyT. Total funding $24,000. 2006-07. Development of an improved DNA vaccine for prevention of bovine viral diarrhea in calves. Co-I with Surya Waghela and Alfredo Sahagun. CONACyT, Total funding $15,780, 2008-09. Scientific Meeting Participation: Abstracts: 1. Properties of rabbit anti-bacteriophage ΦX174 antibodies. G.G. Wagner*, M.J. Freeman, and C.S.

Buller. 67th Annual Meeting of the American Society for Microbiology, 1967. 2. Immunological and physical characterization of foot-and-mouth disease virus concentrated by

polyethylene glycol precipitation. G.G. Wagner*, J.L. Card, K.M. Cowan, and J.H. Graves. Fed. Proc. 28:429 (1969).

3. Neutralizing reactivities of 19S and 7S antibodies to foot-and-mouth disease virus. G.G. Wagner* and K.M. Cowan. Fed. Proc. 29:435 (1970).

4. Application of radial immunodiffusion for the detection of foot-and-mouth disease virus infections. G.Wagner* and K.Cowan. 71st Annual Meeting of the Amer. Soc. for Microbiology, 1971.

5. The immune response to foot-and-mouth disease virus. K.M. Cowan* and G.G. Wagner. Proceedings of the symposium, The Bovine Immune System. J. Dairy Sci. 54:1309 (1971).

6. Antigenic character of Theileria. G.G. Wagner*, W.P.H. Duffus, M. Burridge, M. Lule and B.A. Allsopp. Proceedings of the Third International Congress of Parasitology. 2:1105 (1974).

7. Immunoglobulin responses of cattle to Theileria parva. G.G. Wagner*, W.P.H. Duffus, and M. Lule. Proceedings of the Third Internat. Cong. of Parasitology. 2:1033 (1974).

8. Effects of cyclophosphamide induced immunosuppression on the recrudescence of Anaplasma infection. D.Corrier*, G.Wagner and G.Adams.59th Conf. Res. Workers on Animal Diseases, 1978.

9. Effects of niridazole on the cell-mediated immune response and leukocyte counts of calves. D.Corrier*, G.Adams, G.Wagner and K.Pierce. 60th Conf. Res. Workers on Animal Diseases, 1979.

10. Biochemical parameters of bovine babesiosis. G. Elissalde*, M. Elissalde, G. Wagner, and T. Craig. 25th Annual Meeting of the American Association of Veterinary Parasitologists, 1980.

11. Characterization of a tick stabilate of Babesia bovis. T.M. Craig* and G.G. Wagner. 25th Annual Meeting of the American Association of Veterinary Parasitologists, 1980.

12. Effects of acute Babesia bovis infection on bovine leukocytes. G.G. Wagner*, W. Goff and G.S. Elissalde. Amer. Zool.20:794 (1980).

13. Alterations in complement during acute bovine babesiosis. G. Adams* and G. Wagner. In: The Ruminant Immune System (Adv in Exp.Med. and Biol.Vol.137). Ed. J.Butler, Plenum Press, New York, 1980. p. 793.

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Scientific Meeting Participation – Abstracts (continued): 14. Ultrastructural characterization of a cell line from the tick Bophilus microplus. P. Holman*,

R.Droleskey, and G.Wagner. 32nd Annual Meeting of the Tissue Culture Association, 1981. 15. Babesia bovis infection of a tick cell line studied by electron microscopy. R.E. Droleskey, P.J.

Holman* and G.G. Wagner. 32nd Annual Meeting of the Tissue Culture Association, 1981. 16. Studies on the epidemiology of anaplasmosis. G.G. Wagner*. 2nd Annual Meeting of the Mexican

Assoc. Veterinary Parasitologists, 1981. 17. Antibody-dependent cell-mediated cytotoxicity in sheep. R. Zipern*, R. Wilson, M. Elissalde, D.

Clark and G. Wagner. 81st Annual Meeting, American Society for Microbiology, 1981. 18. Antibody-dependent cell-mediated cytotoxic reactions during Brucella abortus infection in cattle.

G.G. Wagner*, L.G. Adams and F.C. Heck. 34th Annual Brucellosis Research Conference, 1981. 19. Failure of viable BCG to stimulate resistance to Anaplasma marginale infection in calves. D.

Corrier*, G. Adams and G. Wagner. 62nd Conf. Res. Workers on Animal Diseases, 1981. 20. A possible mechanism for the pathogenesis of bovine babesiosis. G. Elissalde*, G. Wagner, M.

Elissalde and T.Craig. 7th National Anaplasmosis Conference, 1981. 21. Immunoglobulin responses of cattle associated with recrudescent Anaplasmosis marginale infections.

G. Wagner. 7th National Anaplasmosis Conference, 1981. 22. Antigen binding buffers in the enzyme-linked immunospecific assay (ELISA) for Anaplasma

marginale antibody activity in bovine sera. R.F. Long and G. Wagner. 7th National Anaplasmosis Conference, 1981.

23. The use of antibody-dependent cell-mediated cytotoxicity, complement fixation and indirect fluorescent antibody tests to determine specific immunoglobulin activity from cattle infected with Babesis bovis. W. Goff, G. Wagner, T. Craig, and R. Long.7th National Anaplasmosis Conference, 1981.

24. The protective effect of killed Corynebacterium parvum against acute babesiosis in mice and calves. D. Corrier*, G. Adams and G. Wagner, 63rd Conf. Res. Workers on Animal Diseases, 1982.

25. Comparison of the IFA test and ELISA for Anaplasma marginale serology in cattle. R. Teclaw*, R. Nordgren and G. Wagner, Spring Meeting, Texas Branch, Amer. Soc. for Microbiol. 1982.

26. Morphology of different stages of Cryptosporidium spp. in avian,bovine and mouse. R.E. Mamer* and G.G. Wagner. 83rd Annual Meeting of the American Society for Microbiology, 1983.

27. Diagnosis of hemoprotozoan infections of cattle. G.G. Wagner*. Mexican Ministry of Agriculture - S.A.R.H., International Symposium on Hemoprotozoan Diseases, 1983.

28. The in vitro observation of the proposed sexual stages of Babesia bovis. R. Droleskey*, P. Holman, T.Craig, H.Mollenhauer and G.Wagner. Texas Branch, Electron Microscopy Society, 1983.

29. Encapsulation of drugs in bovine carrier erythrocytes for treatment of anaplasmosis. J. DeLoach* and G.Wagner. 10th Internat. Symposium on Controlled Release of Bioactive Materials, 1983.

30. Immunodiagnosis of protozoan infections. G.G. Wagner*. Texas A&M University Symposium on Immunoregulation of Parasitic Diseases, 1983.

31. Effect of Infection by Babesia microti on glycolysis in mouse erythrocytes - an in vivo 13-C-NMR spectroscopic approach. N.E. MacKenzie*, J.S. Johnson and G.G. Wagner. 58th Annual Meeting of the American Society of Parasitologists, 1983.

32. Seroepidemiology of babesiosis in northeastern Mexico. R.F. Teclaw and G.G. Wagner*. Foreign Animal Disease Committee, 88th Meeting of the U.S. Animal Health Association, 1984.

33. Vaccine protection against anaplasmosis without induction of persistent post-vaccinal antibody response. D.Corrier*, G.Wagner and J. Johnson. 65th Ann. Conf. Res. Wkr. Anim. Dis., 1984.

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Scientific Meeting Participation – Abstracts (continued): 34. Detection of Plasmodium falciparum and Babesia bovis using repetitive DNA probes. G.

McLaughlin*, T. Edlind, G. Campbell, R. Eller,G. Wagner and G. Ihler. 13th Annual Gulf Coast Molecular Biology Conference, 1984.

35. Babesia bovis diagnosis using plasmids containing repetitive DNA. G. McLaughlin*, T. Edlind, G. Wagner and G. Ihler. 60th Annual Meeting of the American Society of Parasitologists, 1985.

36. Seroepidemiology of Babesia bovis and B. bigemina in cattle in five Mexican states. R. Teclaw, G.Wagner and G.Carranza*. 6th Ann.Meeting, Mexican Assoc. Veterinary Parasitologists, 1985.

37. Anaplasmosis and babesiosis in Nuevo Leon. S. Romo*, R.F. Teclaw and G. Wagner. 6th Annual Meeting of the Mexican Association of Veterinary Parasitologists, 1985.

38. Characterization of the cervine piroplasm, Babesia odocoilei (Emerson). K Waldrup*, P. Holman, R.Droleskey and G.Wagner.19th Ann.Meeting, Southwestern Assoc. of Parasitologists, 1986.

39. Studies of cross-strain protection between two strains of Babesia microti, S.E. Sharp* and G.G. Wagner. Fed. Proc. 45:861 (1986).

40. Fine Structure of continuously cultured Babesia isolated from a white-tailed deer (Odocoileus virginianus). R.Droleskey* P.Holman, K.Waldrup and G.Wagner. Texas Branch, Electron Microscopy Society, 1986.

41. Serological studies of bluetongue incidence in Nuevo Leon, Tamaulipas and Coahuila. S. Romo*, R. Teclaw, G.G. Wagner and Z. Garcia. 12th National Congress of Buiatrics (Mexico), 1986.

42. Prevalence of brucellosis in cattle in northeast Mexico. S. Romo*, R. Teclaw, G.G. Wagner and Z. Garcia. 12th National Congress of Buiatrics (Mexico), 1986.

43. Studies into possible reservoirs of babesiosis in Texas. K. Waldrup* and G. Wagner. National Center for Animal Parasitology (Mexico), Internat. Symposium on Animal Parasitology, 1986.

44. Immune response to Babesia. G.G. Wagner*, C. Tripp and A. Cavazzani. National Center for Animal Parasitology (Mexico), International Symposium on Animal Parasitology, 1986.

45. Expression vector cloning of mung bean nuclease digested Babesia bovis DNA. C. Tripp*, G.G. Wagner and A.C. Ficht. University of Texas, Lost Pines Molecular Biology Conference, 1986.

46. Immune responses of cattle to Babesia. G.G. Wagner* and C Tripp. Annual Meeting, Anaplasmosis Research Workers, 1986.

47. Cloning Babesia bovis genes in the lambda gt11 expression vector. Fall meeting, Texas Branch, American Society for Microbiology, 1986.

48. Expression vector cloning of mung bean nuclease digested Babesia bovis DNA. C. Tripp*, G.G. Wagner and A.C. Ficht. 61st Annual Meeting American Society of Parasitologists, 1986.

49. Serologic response to Cryptosporidia in naturally infected cattle. B.E. Mamer* and G.G. Wagner. 61st Annual Meeting of the American Society of Parasitologists, 1986.

50. In vitro growth of a bovine Babesia in cervine erythrocytes. P.J. Holman*, K.A. Waldrup and G.G. Wagner. 40th Annual Meeting of Animal Disease Research Workers in Southern States, 1987.

51. Ultrastructure of three continuously cultured Babesia species. R.Droleskey*, P.Holman, K. Waldrup, H.Mollenhauer and G.Wagner.40th Ann.Meeting, Anim.Dis.Res.Wkrs S. States, 1987.

52. Comparison of immunosuppression by T-2 toxin, dexamethasone and cyclophosphamide on murine babesiosis. D.Corrier* and G.Wagner. 40th Ann.Mtg, Anim.Dis.Res.Wkrs in Southern States, 1987.

53. Erythrocyte drug carriers for babesiosis chemotherapy. G.G. Wagner*, D.E. Corrier and J.R. DeLoach. 14th International Symposium on Controlled Release of Bioactive Materials, 1987.

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Scientific Meeting Participation – Abstracts (continued): 54. Immunosuppression by T-2 toxin, cyclophosphamide and dexamethasone on Babesia microti

infections in mice. D.Corrier and G.Wagner.68th Ann. Conf. Res.Workers in Animal Disease, 1987.

55. A specific DNA probe for detection of Babesia bovis in blood samples. P.J. Holman*, C.A. Tripp, A.C. Ficht and G.G. Wagner, Eighth National Veterinary Hemoparasite Conference, 1989.

56. Observations on Babesia bovis and Babesia bigemina infected cultures using differential interference contrast microscopy-Nomarski, scanning and transmission electron microscopy, and scanning density microspectrophotometry. D. Kennedy*, R. Droleskey, J. DeLoach, C. Yeverino, R. Alanis, P. Holman and G. Wagner. Eighth National Veterinary Hemoparasite Conference, 1989.

57. A serologic investigation of babesiosis in south Texas cattle herds. R.F. Teclaw*, R. Smith, S. Reynolds and G.Wagner. Eighth National Veterinary Hemoparasite Conference, 1989.

58. Cross-strain protection between lethal and non-lethal strains of Babesia microti. G.Carranza*, S. Sharp, J.Womack and G.Wagner. Eighth National Veterinary Hemoparasite Conference, 1989.

59. Infection of non-domestic ruminants with the etiologic agents of bovine babesiosis. K. Waldrup*, P.J. Holman and G.G. Wagner. Eighth National Veterinary Hemoparasite Conference, 1989.

60. Structure of hemolymph and intraerythrocytic stages of Babesia bovis. R.Droleskey*, P. Holman, D. Cruz, J.DeLoach and G. Wagner. Texas Branch, Electron Microscopy Society, 1990.

61. Macrophage dependent cross protection between a non-lethal and a lethal strain of Babesia microti in mice. G. Wagner*, G. Carranza and S. Sharp. FASEB J. 4:A2191 (1990).

62. Characterization of Babesia bovis antigens which stimulate bovine helper T cells. W.C. Brown*, K.S. Logan, G.G. Wagner and C. Tetzlaff. FASEB J. 4:A2250 (1990).

63. The threat of tick-borne disease to international livestock interests in the western hemisphere. G. Wagner. 127th Annual Meeting of the American Veterinary Medical Association, 60 (1990).

64. Epidemiology, diagnosis and control alternatives for anaplasmosis. G. Wagner*, D. Cruz, P. Holman and S. Waghela. Proceedings, Second International Seminar on Animal Parasitology, Cuernavaca, Morelos, Mexico, 1991.

65. Epidemiology, Diagnosis and Prevention of Bovine Babesiosis. G. Wagner*, D. Cruz, P. Holman and S. Waghela. Proceedings, Mexican National Association for Livestock Research, Ciudad Victoria, Tamaulipas, Mexico, 1991.

66. Anaplasmosis: Epidemiology, diagnosis and alternate control strategies. G. Wagner* Proceedings, International Symposium on Preventative Veterinary Medicine, Monterrey, Nuevo Leon, Mexico, 1992.

67. The effect of confinement in metabolism stalls on cortisol, antibody production, and antibody-dependent cell-mediated cytotoxicity of tame lambs. M. Hsieh*, T. Friend and G. Wagner, American Society of Animal science, 1993.

68. Fine structure of a wildlife ruminant babesia. R. Droleskey*, P. Holman, K. Waldrup, W. Goff, D. Corrier and G. Wagner. Texas Society of Electron Microscopy, 1993.

69. Culture confirmation of Babesia equi infection. P.J. Holman, S.K. Hietala, L.R. Kayashima, D. Olson* and G.G Wagner. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

70. Comparison of a Babesia sp. from desert bighorn sheep with other wild ruminant Babesia spp. and B. odocoilei. W.L. Goff*, D. Alperin, P.J. Holman and G.G Wagner. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

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Scientific Meeting Participation – Abstracts (continued): 71. Comparison of three babesia isolated from cervids. P.J. Holman, T.M. Craig*, S.D. Waghela, R.E.

Droleskey, J.R. DeLoach and G.G. Wagner. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

72. In vitro cultivation of Anaplasma marginale. S.D. Waghela, D. Melendy, D. Cruz, R.E. Droleskey, and G.G. Wagner*. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

73. Isolation of a high molecular weight protein of Babesia bovis with monoclonal antibodies. A.S. Sahagun*, S.D. Waghela, and G.G. Wagner. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

74. Cell culture derived vaccine for babesiosis. G. Wagner*, P. Holman, D. Cruz, S. Waghela, A.Sahagun, A.Snydelaar, J.Dominguez, and I.Rodriguez. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

75. Ultrastructural characterization of a Babesia isolated froma naturally infected desert bighorn sheep (Ovis canadensis nelsoni). R. Droleskey, P. Holman, K. Waldrup, W. Goff, J. DeLoach and G. Wagner*. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

76. Babesia caballi carrier horses identified by culture. P. Holman, D. Melendy*, L. Chieves and G. Wagner. Proceedings of the Ninth International Veterinary Hemoparasite Disease Conference, Merida, Yucatan, Mexico, 1993.

77. Fine structure of a wildlife ruminant babesia. R. Droleskey*, P. Holman, K. Waldrup, W. Goff, D. Corrier and G. Wagner. Texas Branch, Electron Microscope Society, 1994.

78. The pattern of antibody activity against Babesia bovis in western blot. (Abstract). A. Sahagun*, S. Waghela, P. Holman and G. Wagner. 69th Annual Meeting of the American Society of Parasitologists, Ft. Collins, Colorado, 1994.

79. A cloned DNA probe for identification of Babesia caballi in infected horses. A. Sahagun*, S.D. Waghela, P. Holman, and G.Wagner. Proceedings, XIV Congreso Panamericano de Ciencias Veterinarias, Acapulco, Guerrero, Mexico, 1994.

80. Babesia Vaccine Trials in Mexico. G. Wagner*, P. Holman, D. Cruz, S. Waghela, A. Sahagun, A. Snydelaar, J. Medellin, J Dominguez, and I. Rodriguez. Proceedings, 8th International Congress of Parasitology, Izmir, Turkey, 1994.

81. Cloning and characterization of a Babesia caballi specific DNA probe. A. Sahagun*, S. Waghela, and G.Wagner. 76th Annual Meeting of the Conference of Research Workers in Animal Disease. Chicago, Illinois, 1995.

82. Cloning and expression of a Babesia caballi protein froma a genomic library in lambda Zap II. A. Sahagun*, S.Waghela, and G. Wagner. Proceedings, Reunion Nacional de Investigacion Pecuaria, Mexico City, Mexico, 1995.

83. Cloning and expression of a Babesia caballi protein from a genomic library in lambda Zap II. A. Sahagun*, S. Waghela, and G.Wagner. Proceedings, Reunion Nacional de Investigacion Pecuaria, Mexico City, Mexico, 1995.

84. The role of esterases in the mechanism of resistance to acaricides in Boophilus microplus ticks. R. Rosario*, G. Wagner and J. George. III Seminario Internacional de Parasitologia Animal, Acapulco, Guerrero, Mexico, 1995.

21

Scientific Meeting Participation – Abstracts (continued): 85. Preliminary studies on Theileria infections of cattle. P. Holman, J. Chae, S. Waghela, M. Levy, and

G. Wagner*. Proceedings, 99th Annual Meeting of U.S. Animal Health Association, Reno, Nevada 1995.

86. Characterization of a high molecular weight antigen common to various isolates of Babesia bovis. A. Sahagun*, S.Waghela, M. Romany, P. Holman, D. Melendy, D. Cruz and G. Wagner. Proceedings, XXVI Congreso Nacional de Microbiologia. Acapulco, Guerrero, Mexico, 1996.

87. Cloning and expression of a Babesia caballi protein from a genomic library in lambda Zap II. A. Sahagun*, S.Waghela, and G.Wagner. 77th Annual Meeting of the Conference of Research Workers in Animal Disease. Chicago, Illinois, 1996.

88. Cysteine Proteases of Babesia equi Identified using Substrate Gel SDS-Page and Specific Inhibitors. M. Hsieh*, S. Waghela, P. Holman and G. Wagner. Lost Pines Annual Conference on Molecular Biology, Bastrop, Texas, 1998.

89. Organophosphate degradation activity in the acaricide resistant cattle tick Boophilus microplus E. Miranda*, R. Cossio, S. Waghela and G. Wagner. Lost Pines Annual Conference on Molecular Biology, Bastrop, Texas, 1998.

90. Generation of a Boophilus microplus organophosphate resistant cell line. R. Cossio*, G. Wagner and P. Holman. Lost Pines Annual Conference on Molecular Biology, Bastrop, Texas, 1998.

91. Molecular characterization of Theileria cervi from a White-tailed Deer (Odocoileus virginianus). A. Johnson*, R. Cossio-Bayugar, D. Cruz, J. Chae, G. Wagner and P. Holman. Howard Hughes Science Conference, College Station, Texas, 1998.

92. Cysteine Proteases of Babesia equi Identified using Substrate Gel SDS-Page and Specific Inhibitors. M. Hsieh*, S. Waghela, P. Holman and G. Wagner. Amer. Soc. for Microbiology, Texas Branch Meeting, College Station, Texas, 1998.

93. Babesia equi infected horses detected by culture, complement fixation and indirect fluorescent antibody techniques. P. Holman*, D. Cruz, T. Becu, and G. Wagner. IX International Symposium of Veterinary Laboratory Diagnosticians/OIE Seminar on Biotechnology. College Station, Texas, 1999.

94. Theileria cervi and Babesia odocoilei infections detected in deer hosts by specific ssu rRNA gene fragment amplification. P. Holman*, J. Bennett, D. Cruz, and G. Wagner. IX Internat. Symp. of Veterinary Laboratory Diagnosticians/OIE Seminar on Biotechnology. College Station, Texas, 1999.

95. Molecular analysis of the small subunit ribosomal RNA gene from elk hemoparasites Babesia odocoilei and Theileria cervi. Kristy Rialon*, G. Wagner and P. Holman. NASA-SHARP Summer program at Texas A&M University. College Station, Texas, 1999.

96. Economic impact of Boophilus microplus infestation in two Mexican tropical cattle farms. M. Villarino* and G. Wagner. Proc., 4th Internat. Sem. on Animal Parasitology, Puerto Vallarta, Jalisco, Mexico, 1999.

97. Cattle tick gene expression in the baculovirus system. E. Miranda*, R. Cossio, J. George, S. Waghela and G. Wagner. Proceedings, 4th Internat. Seminar on Animal Parasitology, Puerto Vallarta, Jalisco, Mexico, 1999.

98. Esterase genes in Boophilus microplus: Differences between susceptible and ixodicide resistant ticks. R. Hernandez*, H. He. A. Chen, S. Waghela, W. Ivie, J. George and G. Wagner. Proceedings, 4th International Seminar on Animal Parasitology, Pto Vallarta, Jalisco, Mexico, 1999.

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Scientific Meeting Participation – Abstracts (continued): 99. Differential display studies of organophosphate resistance in cultured Boophilus microplus cells. R.

Cossio*, G. Wagner and P. Holman. Proceedings, 4th International Seminar on Animal Parasitology, Puerto Vallarta, Jalisco, Mexico, 1999.

100. “New” diagnostic tests: Parasites or antibody? P. Holman, D. Cruz and G. Wagner*. Proc. 4th International Seminar on Animal Parasitology, Puerto Vallarta, Jalisco, Mexico, 1999.

101. Detection of Esterase B in Culex quinquefasciatus (Say), Boophilus microplus (Canestrini), Amblyomma cajennense (Fabricius), and Blatella germanica (L) with the use of Zymograms. M. Villarino*, S. Waghela, J. Olson and G. Wagner. 17th Southern Conference of Animal Parsitologists, Columbus, Georgia, April, 2000.

102. Prueba de PCR para la Deteccion de Alelos Mutantes en un Gen de Esterasa en Garrapatas Individuales Boophilus microplus. R. Hernandez*, J. George, Z. Garcia and G. Wagner. Proc. XIV National Congress of Parasitology, Guadalajara, Jalisco, Mexico, October, 2000.

103. Aislamiento de campo de una cepa local de babesia bigemina para el establecimiento del cultivo in vitro.” R. Rodríguez*, P. Quiniones, G. Ramirez, D. Cruz and G. Wagner. Proc. XIV National Congress of Parasitology, Guadalajara, Jalisco, Mexico, October, 2000.

104. Expresion Aumentada de un Gen de Esterasa en una Cepa Resistente a los Piretroides de la Garrapata Boophilus microplus.” R. Hernandez*, F. Guerrero, J. George, Z. Garcia and G. Wagner. Proc. XIV National Congress of Parasitology, Guadalajara, Jalisco, Mexico, October, 2000.

105. Bioterrorism: An International Challenge to Animal Agriculture. G. Wagner. Annual Meeting of the National Institute of Animal Agriculture, Colorado Springs, Colorado, April, 2001.

106. Update on Parasitic Diseases Foreign to the U.S. G. Wagner. Annual Meeting of the National Institute of Animal Agriculture, Colorado Springs, Colorado, April, 2001.

107. Update on Parasitic Diseases Foreign to the U.S. G. Wagner. USDA Foreign Animal Disease Diagnosticians course, Plum Island Animal Disease Center, Greenport, New York, October, 2001.

108. Cell culture establishment of a local (Yucatan) strain of Babesia bigemina. I. Rodriguez, J. Quiniones, G. Ramirez, D. Cruz and G. Wagner. Proc. XV National Congress of Parasitology, Merida, Yucatan, Mexico, October, 2002.

109. Case Review: Presumed Babesiosis in a North American Elk. A. Irizarry-Rovira, L. Gallatin, P. Holman, M. Renninger, G. Wagner, J. Sojka, and J. Christian. Annual Meeting of the American Society for Veterinary Clinical Pathology, Banff, Alberta, Canada, November, 2003.

110. Cryptosporidium parvum Infection Up-regulates the Gene Expression of Glycolytic Enzymes in Host Cells. X. Cai, S. Waghela, G. Wagner and G. Zhu. 110th Annual Meeting of the American Society for Microbiology, San Diego, California, May, 2004.

111. Update on Texas cattle fever (babesiosis) and cattle fever ticks (Boophilus microplus and B. annulatus). Cattle Health and Well-being Committee, Annual meeting of the National Cattlemen's Beef Association, Denver, Colorado, February, 2006.

112. Update on Texas cattle fever (babesiosis) and cattle fever ticks (Boophilus microplus and B. annulatus). Ticks and Tick-borne Diseses Committee, Annual meeting of the U.S. Animal Health Association, Reno, NV, October, 2007.

112. Preventing colibacillosis diarrhea in calves with anti-F5 recombinant antibodies produced in rice plants. Texas A&M University – CONACyT Principal Investigators Symposium, College Station, TX, February, 2008.

23

Scientific Meeting Participation – Abstracts (continued): 113. Effects of foreign animal disease outbreaks on United Kingdom animal health policy: Implications

for a US emergency response. A. Delgado, B. Norby, H.M. Scott and G. Wagner. Department of Homeland Security, Science and Technology, Annual Research and Education Summit, Washington, D.C., March, 2008

114. Shift in rules of engagement for eradication of cattle fever ticks in the United States. Annual meeting of the American Association of Veterinary Parasitology, Atlanta, GA, August, 2010.

Refereed Proceedings: 1. Proceedings of the symposium, The Bovine Immune System. J.E. Butler, A.J. Winter, and G.G.

Wagner. J. Dairy Sci. 54:1309-1340 (1971). 2. A possible mechanism for the pathogenesis of bovine babesiosis. G. Elissalde, G. Wagner, T.M. Craig

and M. Elissalde. In: Proceedings 7th Natl. Anaplas. Conf. (1981): 57-80. 3. Enhancement of antigen binding in the enzyme-linked immunospecific assay (ELISA) for Anaplasma

marginale antibody activity in bovine serum. R.F. Long and G.G. Wagner. In: Proceedings 7th Nat. Anaplas. Conf. (1981): 321-331.

4. The use of antibody-dependent cell-mediated cytotoxicity, complement fixation and indirect fluorescent antibody tests to determine specific immunoglobulin levels in cattle infected with Babesia bovis. W. Goff, G.G. Wagner, T.M. Craig and R. Long. In: Proceedings 7th Natl. Anaplas. Conf. (1981): 369-390.

5. Immunoglobulin responses of cattle associated with recrudescent Anaplasma marginale infection. G.G. Wagner. In: Proceedings 7th Natl. Anaplas. Conf. (1981): 307-320.

6. Rapid card test for bovine babesiosis. L. Chieves, G. Wagner, P. Byers and W.M. Frerichs. In: Proceedings 8th Natl. Hemopar. Conf. (1989): 341-351.

7. Vulnerability of U.S. livestock to tick-borne diseases. G. Wagner. In: Proceedings U.S. Anim. Hlth Assn. 93:270-272 (1989).

8. Non-immunological methods of diagnosis of babesiosis. G. Wagner, D. Cruz, P. Holman, S. Waghela, J. Perrone, S. Shompole and F. Rurangirwa. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, 87:193-199 (1992).

9. The Threat of Tick-Borne Disease to International Livestock Interests in the Western Hemisphere. In: Proceedings Food Animal Policy Research Institute Biennial Economics Conference. (2003).

10. Babesia and Theileria infections in wildlife. G. Wagner and P. Holman. In: Proceedings U.S. Anim. Hlth Assn. 107, 231 (2003).

11. Science, Politics and Texas Cattle Fever. G. Wagner. U.S. Cattle Fever Tick Eradication Program: 100 years of protecting the U.S. cattle industry from Texas Cattle Fever. In: Proceedings: 100th Anniversary of the U.S. Tick Eradication Program, Kingsville, TX, April, 2007.

12. One Health approach to identify research needs in bovine and human babesioses: A. Perez de Leon, et al. Workshop Report. Parasites & Vectors, 3:36-46 (2010).

Bibliography:: Scientific Publications in Refereed Journals: 1. Quantitative aspects of the antibody response of rabbits to bacteriophage ΦX174. G.G. Wagner and

J.M. Freeman. Int. Arch. Allergy Appl. Immun. 37:432-439 (1970). 2. Heterogeneity of the antibody responses of rabbits to bacteriophage ΦX174. G.G. Wagner and M.J.

Freeman. Int. Arch. Allergy Appl. Immun. 37:449-457 (1970). 3. Immunochemical studies of foot-and-mouth disease. VII. Characterization of foot-and-mouth disease

virus concentrated by polyethylene glycol precipitation. G.G. Wagner, J.L. Card, and K.M. Cowan. Arch. Ges. Virus-forsch. 30:343-352 (1970).

24

Bibliography -- Scientific Publications in Refereed Journals (continued): 4. Foot-and-mouth disease virus antibodies: Comparison of a tissue culture microneutralization test with

the assay in suckling mice. G.G. Wagner and J.W. McVicar. Appl. Microbiol. 20:995-997 (1970). 5. Immunochemical studies of foot-and-mouth disease VIII. Detection and quantitation of antibodies by

radial immunodiffusion. K.M. Cowan and G.G. Wagner. J. Immunol. 105:557-566 (1970). 6. Immunochemical studies of foot-and-mouth disease. IX. Differences in neutralizing activities of

guinea pig and bovine 19S and 7S antibodies. G.Wagner and K.Cowan. J. Immunol. 106:656-660 (1971).

7. Data processing for radial immunodiffusion. R. Trautman, K.M. Cowan, and G.G. Wagner. Immunochemistry. 8:901-916 (1971).

8. Latent virus infection in contact transmission of foot-and-mouth disease from infected to susceptible cattle. J.H. Graves, J.W. McVicar, P. Sutmoller, R. Trautman and G.G. Wagner. J. Infect. Dis. 124:270-276 (1971).

9. Screening of sera for antibodies to foot-and-mouth viral antigen. G.G. Wagner, K.M. Cowan, and J.W. McVicar. Inf. and Imm. 5:227-231 (1972).

10. Theileriosis: The exposure of immunized cattle in a Theileria lawrencei enzootic area. M.P. Cunningham, C.G.D. Brown, M.J. Burridge, A. Irvin, I. Kirimi, R.E. Purnell, D.E. Radley, and G.G. Wagner. Trop. Anim. Hlth. Prod. 6:39-43 (1974).

11. The specific immunoglobulin response in cattle immunized with Theileria parva (Muguga) stabilate. W.P.H. Duffus, and G.G. Wagner. Parasitology. 69:31-41 (1974).

12. The specific immunoglobulin response in cattle immunized with isolated Theileria parva antigens. G.G. Wagner, W.P.H. Duffus, and M.J. Burridge. Parasitology. 69:43-53 (1974).

13. Leukocyte migration inhibition as a model for the demonstration of delayed hypersensitivity in East Coast Fever. S.I. Muhammed, G.G. Wagner, and L.H. Lauerman. Immunology. 27:1033-1037 (1974).

14. Immunochemical studies on East Coast Fever I. Partial segregation and characterization of the Theileria parva schizont antigen. G.G. Wagner, C.G.D. Brown, W.P.H. Duffus, C.D. Kimber, J.C. Crawford, and M. Lule. J. Parasitol. 60:848-853 (1974).

15. Immunological studies on East Coast Fever II. Partial segregation and characterization of the Theileria parva piroplasm antigen. G.G. Wagner, W.P.H. Duffus, C.D. Kimber, and M. Lule. J. Parasitol. 60:854-859 (1974).

16. Immunochemical studies on East Coast Fever III. Development of the indirect hemagglutination assay using Theileria parva piroplasm antigen. W.P.H. Duffus, and G.G. Wagner. J. Parasitol. 60:860-865 (1974).

17. The specific immune response in the lymph nodes of cattle undergoing Theileria parva infection, as determined by the rosette test. P. Duffus, and G. Wagner. In Parasitic Zoonoses, Clinical and Experimental Studies. Ed. Soulsby, Academic Press, New York, 1974, pp. 85-95.

18. Anti-lymphocyte antibody response in cattle inoculated with theileria parva infected lymphoblastoid cell lines. G. Wagner and P. Duffus. In Parasitic Zoonoses, Clinical and Experimental Studies. Ed. Soulsby, Academic Press, New York, 1974, pp. 97-107.

19. The specific immunoglobulin response in cattle to Theileria parva (Muguga) infection. G.G. Wagner, and W.P.H. Duffus. Parasitology. 70:95-102 (1975).

20. Diminished antibody response to rinderpest vaccination in cattle undergoing experimental East Coast Fever. G.G. Wagner, D.H. Jessett, D.E. Radley, and C.G.D. Brown. Res. Vet. Sci. 19:209-211 (1975).

21. Purification and characterization of precipitating antigens from Theileria parva. B.A. Allsopp, G.G. Wagner, K.P. Matthews, and C.G. Kariavu. J. Gen. Microbiol. 100:319-328 (1977).

22. East Coast Fever: Cultivation in vitro of cell-free schizonts and merozoites of Theileria parva and their immunogenicity in cattle. M.B.A. Nyindo, J.S. Kaminjolo, G.G. Wagner, and M. Lule. Am. J. Vet. Res. 39:37-44 (1978).

25

Bibliography -- Scientific Publications in Refereed Journals (continued): 23. Antibody-dependent cell-mediated cytotoxicity in the bovine: Activity against 51-Cr-labelled

chicken red blood cells coated with protozoal antigens. W.P.H. Duffus, A.E. Butterworth, G.G. Wagner, J.M. Preston, and D. Franks. Inf. and Imm. 22:492-501 (1978).

24. Initial studies on the properties of a bovine lymphoid cell culture line infected with Theileria parva. W.P.H. Duffus, G.G. Wagner, and J.M. Preston. Clin. Exptl. Immunol. 34:347-353 (1978).

25. Study of sonicated extracts and saline washes of Brucella melitensis in the agar gel immunodiffusion test. S. Waghela, J.G. Wandera and G.G. Wagner. Kenya Veterinarian. 3:13-16 (1979).

26. Sonic extracts of Brucella melitensis as antigens in complement fixation and indirect hemagglutination tests. S. Waghela, J. Wandera and G.G. Wagner. Kenya Veterinarian. 4:11-12 (1980).

27. Comparison between certain serological tests for diagnosis of East Coast Fever. W.P.H. Duffus and G.G. Wagner. Vet. Parasitol. 6:313-324 (1980).

28. Bovine babesiosis in northern Mexico. G.D. Thompson, J.A. Medellin, G.S. Trevino and G.G. Wagner. Trop. Anim. Hlth. Prod. 12:132-136 (1980).

29. Comparison of four serological tests in diagnosis of caprine brucellosis. S. Waghela, J.G. Wandera and G.G. Wagner. Res. Vet. Sci. 28:168-171 (1980).

30. Prevention of brucellosis in dairy cattle. L.G. Adams, R.P. Crawford, D.S. Davis, F.C. Heck, G.G. Wagner, J.D. Williams and D.L. Zink. Dairy Research in Texas, TAES PR-3751, 108-111 (1980).

31. Recrudescence of Anaplasma marginale induced by immunosuppression with cyclophosphamide. D.E. Corrier, G.G. Wagner and L.G. Adams. Amer. J. Vet. Res. 42:19-21 (1981).

32. Serological prevalence of bovine babesiosis in Guyana. L.M. Applewhaite, T.M. Craig and G.G. Wagner. Trop Anim. Hlth. Prod. 13:13-18 (1981).

33. Prevention of brucellosis in beef cattle. L.G. Adams, R.P. Crawford, D.S. Davis, F.C. Heck, G.G. Wagner, J.D. Williams and D.L. Zink. Beef Research in Texas, TAES PR-3804, 125-127 (1981).

34. Ultrastructure of Babesia bovis (Babes, 1880). R. Todorovic, G.G. Wagner and M. Kopf. Vet. Parasitol. 8:277-290 (1981).

35. Imidocarb dipropionate encapsulation and binding to resealed carrier bovine erythrocytes for potential babesiosis chemotherapy. J. DeLoach, G. Wagner, and T. Craig. J. Appl.Biochem. 3:254-262 (1981).

36. Clinical and serological evidence of bovine babesiosis and anaplasmosis in Saint Lucia. R.T. Knowles, M. Montrose, T.M. Craig, G.G. Wagner and R.F. Long. Vet. Parasitol. 10:307-311 (1982).

37. The bovine immune response to tick-derived Babesia bovis infection: Serological studies of isolated immunoglobulins. W. Goff, G. Wagner, T. Craig and R. Long. Vet. Parasitol. 11:109-120 (1982).

38. Survival of carrier erythrocytes in splenectomized calves. J.R. DeLoach, and G.G. Wagner. Amer. J. Vet. Res. 44:751-754 (1983).

39. Ultrastructure of Babesia bovis sexual stages as observed in Boophilus microplus cell cultures. R.L. Droleskey, P.J. Holman, T.M. Craig, G.G. Wagner and H.H. Mollenhauer. Res. Vet. Sci. 34:249-251 (1983).

40. Bovine hemoparasitic diseases in Jamaica. D.A.P. Bundy, G. Hylton and G.G. Wagner. Trop. Anim. Hlth. Prod. 15:47-48 (1983).

41. Hypocholesterolemia and hypocortisolemia in acute and terminal Babesia bovis infections. E.S. Elissalde, G.G. Wagner, T.M. Craig, M.H. Elissalde and L. Rowe. Vet. Parasitol. 12:1-11 (1983).

42. Derivation of monoclonal antibodies against Brucella abortus antigens. P. Holman, L. Adams, D. Hunter, F. Heck, K. Nielsen, and G.Wagner. Vet. Immunol. Immunopath. 4:603-613 (1983).

43. Serological prevalence of bovine babesiosis in Mali. D.K. Miller, O. Diall, T.M. Craig and G.G. Wagner. Trop. Anim. Hlth. Prod. 16:71-77 (1984).

26

Bibliography -- Scientific Publications in Refereed Journals (continued): 44. The role of specific immunoglobulins in antibody-dependent cell-mediated cytotoxicity assays during

Babesia bovis infection. W.L. Goff, G.G. Wagner, T.M. Craig and R.F. Long. Vet. Parasitol. 14:117-128 (1984).

45. Comparative assessment of antibody isotypes to Brucella abortus by primary and secondary binding assays. K.N. Nielsen, F. Heck, G. Wagner, J. Stiller, B. Rosenbaum, R. Pugh and E. Flores. Prev. Vet. Med. 2:197-204 (1984).

46. The protective effect of pretreatment with killed Corynebacterium parvum against acute babesiosis in calves. D.E. Corrier and G.G. Wagner. Vet. Parasitol. 15:165-168 (1984).

47. Pharmacokinetics of tetracycline encapsulated in bovine carrier erythrocytes. J.R. DeLoach and G.G. Wagner. Amer. J. Vet. Res. 45:640-642 (1984).

48. 13-C-NMR studies of glycolysis in intra- and extra-erythrocytic Babesia microti. N.E. Mackenzie, J. Johnson, G. Burton, G.G. Wagner and A.I. Scott. Molec. Biochem. Parasitol. 13:13-20 (1984).

49. Increased activity of bovine ADCC effector cells during acute Babesia bovis infection. W.L. Goff, G.G. Wagner and T.M. Craig. Vet. Parasitol. 16:5-15 (1984).

50.. Adverse effects of antibiotics on the development of gut-associated lymphoid tissues and the serum immunoglobulins in chickens. S.A. Naqi, N. Sahin, G. Wagner and J. Williams. Amer. J. Vet. Res. 45:1425-1429 (1984).

51. Demonstration of vaccine-induced immunity to anaplasmosis without induction of persistant postvaccinal complement-fixing and agglutinating antibodies in yearling steers. D.E. Corrier, J.S. Johnson and G.G. Wagner. Amer. J. Vet. Res. 46:583-586 (1985).

52. Viability and virulence of Babesia rodhaini eight years after cryogenic preservation with dimethyl sulfoxide. D.E. Corrier, G.G. Wagner and K.L. Kuttler. Vet. Parasitol. 18:71-73 (1985).

53. A seroepidemiological study of bovine babesiosis in the Mexican states of Nuevo Leon, Tamaulipas and Coahuila. R.F. Teclaw, S. Romo, Z. Garcia, M. Casteneda and G. G. Wagner. Prev. Vet. Med. 3:403-416 (1985).

54. A serological survey for anaplasmosis in cattle in the Mexican states of Nuevo Leon, Tamaulipas and Coahuila using the card test. R.F. Teclaw, S. Romo, Z. Garcia and G.G. Wagner. Prev. Vet. Med. 3:417-426 (1985).

55. Incidence of babesiosis and anaplasmosis in cattle sampled monthly in the Mexican states of Nuevo Leon and San Luis Potosi. R.F. Teclaw, S. Romo, Z. Garcia and G. G. Wagner. Prev. Vet. Med. 3:427-436 (1985).

56. Serologic study of the incidence and prevalence of bluetongue infections in cattle in the Mexican states of Nuevo Leon, Tamaulipas, Coahuila and San Luis Potosi. R.F. Teclaw, S. Romo, S. McConnell and G.G. Wagner. Prev. Vet. Med. 3:437-444 (1985).

57. Prevalence of brucellosis infections in cattle in the Mexican states of Nuevo Leon, Tamaulipas and Coahuila as determined by the ELISA. R.F. Teclaw, S. Romo, F.C. Heck and G.G. Wagner. Prev. Vet. Med. 3:445-448 (1985).

58. The use of vacuum in processing intact ticks for histology. G.A. Carranza, L.M. Martinez, R.M. Rios and G.G. Wagner. J. Parasitol. 73:870-872 (1987).

59. In vitro cultivation of a Babesia isolated from a white-tailed deer (Odocoileus virginianus). P.J. Holman, K.A. Waldrup and G.G. Wagner. J. Parasitol. 74:111-115 (1988).

60. Effect of carrier erythrocytes containing inositol hexaphosphate on Babesia microti infections. J.R. DeLoach, D.E. Corrier and G.G. Wagner. Res. Vet. Sci. 45:262-263 (1988).

61. Comparison of the effect of T-2 toxin with that of dexamethone or cyclophosphamide on resistance to Babesia microti infection in mice. D. Corrier and G. Wagner. Amer. J. Vet. Res. 49:2000-2003 (1988).

62. Erythrocyte drug carriers for babesiosis chemotherapy. G.G, Wagner, D.E. Corrier and J.R. DeLoach. J. Cont. Rel. 8:79-83 (1988).

27

Bibliography -- Scientific Publications in Refereed Journals (continued): 63. Some effects of the trypanocidal drug isometamidium on encapsulation in bovine carrier

erythrocytes. J.R. DeLoach and G.G. Wagner. Biotech. Appl. Biochem. 10:447-453 (1988). 64. Prevalence of erythrocytic protozoa and serologic reactivity to selected pathogens in deer in Texas.

K. Waldrup, E. Collisson, S. Bentsen, C. Winkler and G. Wagner. Prev. Vet. Med. 7:49-58 (1989). 65. Serological prevalence and isolation of Babesia odocoilei among white-tailed deer in Texas and

Oklahoma. K. Waldrup, A. Kocan, T. Qureshi, D.Davis and G.Wagner. J. Wild. Dis. 25:192-201 (1989).

66. Babesia bovis: Gene isolation and characterization using a mung bean nuclease derived espression library. C.A. Tripp, G.G. Wagner and A.C0. Rice-Ficht. Exp. Parasitol. 69:211-225 (1989).

67. Pharamokinetics of imidocarb dipropionate encapsulated in carrier erythrocytes and use of carrier cells for babesiosis chemotherapy. J. DeLoach, G. Wagner and D. Corrier. J. Cont. Rel. 9:243-248 (1989).

68. Bovine Babesia bigemina seroreactors in Jamaica. B. McGinnis, G. Grant, M. Hugh-Jones and G. Wagner. Trop. Anim. Hlth. Prod. 21:227-228 (1989).

69. Microwave fixation and polyester wax embedding for Boophilus microplus histoanatomy. G. Carranza, K. Waldrup, D. Cruz and G. Wagner. J. Med. Ent. 27:1067-1070 (1990)

70. Transmission of Babesia odocoilei in white-tailed deer (Odocoileus virginianus) by Ixodes scapularis (Acari:Ixodidae). K. Waldrup, A Kocan, R. Barker and G. Wagner. J. Wild. Dis. 26:390-391 (1990).

71. Encapsulation and kinetics of muramyl dipeptide in murine carrier erythrocytes and attempted treatment of Babesia in mice with free and encapsulated drug. J.R. DeLoach, K. Andrews, S. Sharp and G. Wagner. Adv. Biosci. 81:67-71 (1991).

****72. Comparative studies of Babesia spp. from white-tailed and sika deer. J.S. Gray, T.M. Murphy, K.A. Waldrup, G.G. Wagner, D.A. Blewett and R. Harrington. J. Wild. Dis. 27:86-91 (1991).

71. Cell-mediated immune responses to Babesia bovis merozoite antigens in cattle following infection with tick-derived or cultured parasites. W.C. Brown, K.S. Logan, G.G. Wagner and C.L. Tetzlaff. Inf. Imm. 59:2418-2426 (1991).

72. Quantitation of Babesia bigemina infections in nymphal and adult Boophilus microplus using a DNA probe. J.L. Hodgson, D. Stiller, D.P. Jasmer, G.M.Buening, G.G. Wagner and T.C. McGuire. Exp. Parasitol. 74:117-126 (1992).

73. Monthly incidence of Theileria cervi and seroconversion to Babesia odocoilei in white-tailed deer (Odocoileus virginianus) in Texas. K.A. Waldrup, J, Moritz, D. Baggett, S. Magyar and G.G. Wagner. J. Wild. Dis. 28:457-459 (1992).

74. In vitro growth of Babesia bovis in white-tailed deer (Odocoileus virginianus) erythrocytes. P.J. Holman, K. waldrup, R. Droleskey, D. Corrier and G. Wagner. J. Parasitol., 79:233-237 (1993)

75. Culture confirmation of the carrier status of Babesia caballi infected horses. P.J. Holman, W. Frerichs, L. Chieves and G. Wagner. J. Clin. Microbiol. 31:698-701 (1993).

76. Carrier red cells from white-tailed deer: Morphology and circulating survival of sickled erythrocytes. J.DeLoach, K.Andrews, R.Droleskey, K. Waldrup and G.Wagner.Res.Vet.Sci., 54:227-234 (1993).

77. The isolation and partial characterization of a babesia spp. from north American desert bighorn sheep (Ovis canadensis nelsoni). W. Goff, D. Jessup, K. Waldrup, J. Thomford, P. Conrad, W. Boyce, J. Gorham and G. Wagner. J. Euk. Microbiol. 40:237-243 (1993).

78. Ultrastructural characteristics of Babesia odocoilei in vitro. R. Droleskey, P. Holman, K. Waldrup, D. Corrier and G. Wagner. J. Parasitol. 79:424-434 (1993).

79. Babesia equi erythrocytic stage continuously cultured in an enriched medium. P. Holman, L. Chieves, W. Frerichs, D. Olson and G. Wagner. J. Parasitol. 80:232-236 (1994).

80. In vitro isolation and cultivation of a babesia from an American woodland caribou (Rangifer tarandus caribou).P. Holman, K. Petrini, J. Rhyan and G. Wagner. J. Wild. Dis. 30:195-200 (1994).

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Bibliography -- Scientific Publications in Refereed Journals (continued): 81. Culture isolation and partial characterization of a babesia from a North American elk (Cervus

elaphus). P. Holman, T. Craig, D. Doan, K. Petrini, J. Rhyan and G. Wagner. J. Wild. Dis. 30:460-465 (1994).

82. Enzyme-linked immunoelectrotransfer blot analysis of excretory-secretory protein of Fascioloides magna and Fasciola hepatica. T. Qureshi, G. Wagner, L. Drawe, D. Davis and T. Craig. Vet. Parasitol. 58:357-363 (1995).

83. Fatal babesiosis in an American woodland caribou (Rangifer tarandus caribou). K. Petrini, P. Holman, J. Rhyan, N. Reindl, P. Wolff, J. Schlater, S. Jenkins and G. Wagner. J. Zoo. and Wild. Med. 26:298-305 (1995).

84. Effect of confinement in metabolism stalls on cortisol, antibody production and antibody-dependent cell-mediated cytotoxicity of lambs.M Hsieh, T Friend, D Lay and G Wagner. J.Anim.Sci.35:52-56 (1996).

85. Development and validation of an indirect enzyme ummunoassay for detection of antibody to Anaplasma marginale in bovine sera. K. Nielsen, P. Smith, D. Gall, S. de Eshaide, G. Wagner and A. Dajer. Vet. Parasitol. 67:133-142 (1996).

86. Field-acquired subclinical Babesia equi infection confirmed by in vitro culture. P. Holman, S. Hietala, L.Kayashima, D.Olson, S.Waghela, and G.Wagner. J.Clin. Microbiol. 35:474-476 (1997).

87. In vitro cultivation of Anaplasma marginale in bovine erythrocytes co-cultured with endothelial cells. S.Waghela, D.Cruz, R.Droleskey, R.DeLoach and G.Wagner. Vet. Parasitol.,73:43-52 (1997).

88. Biotin-labeled DNA probe: A PCR-based assay increases detection sensitivity for Babesia caballi. A. Sahagun, S.Waghela, P. Holman, L. Chieves, and G. Wagner. Vet. Parasitol., 73:53-63 (1997).

89. Identical Small Subunit Ribosomal RNA Gene Nucleotide Sequence from Korean and Japanese Bovine Theileria Isolates and Theileria buffeli (Marula, Kenya). J.S. Chae, O.D. Kwon, P.J. Holman, S.D. Waghela, G.G.Wagner and J.M. Lee. Korean J. Parasitol., 36:47-53 (1998).

90. Nucleotide Sequence Heterogeneity in the Small Subunit Ribosomal RNA Gene Variable (V4) Region among and within Geographic Isolates of Theileria from Cattle, Elk, and White-tailed Deer. J. Chae, J.Lee, O. Kwon, P. Holman, S. Waghela, and G.Wagner. Vet. Parasitol., 75:41-52 (1998).

91. Babesia equi Field Isolates Cultured from Horse Blood using a Microcentrifuge Method. P. J. Holman, T. Becu, E. Bakos, G. Polledo, and G.G. Wagner. J. Parasitol., 84:696-699 (1998).

92. Comparison of Genomic DNA in Various Strains of Boophilus microplus (Acari: Ixodidae). R. Hernandez, A. Chen, R. Davey, W. Ivie, G. Wagner and J.George. J. Med. Ent., 35:895-900 (1998). 93. Studies on the Effect of Infection by Babesia sp. on Oviposition of Boophilus microplus Engorged

Females Naturally Infected in the Mexican Tropics. J. Cen-Aguilar, I Rodriguez-Vivas, J. Dominguez-Alpizar and G. Wagner. Vet. Parastiol. 78: 253-257 (1998).

94. Identification and Sequence Analysis of Small Subunit ribosomal RNA Gene of Bovine Theileria Isolates from Korea and Japan. J. Chae, J. Park, O. Kwon, S. Waghela, P. Holman, G. Wagner and J. Lee. Korean J. Vet. Res., 38:909-917 (1998).

95. Theileria Spp. Infections Associated with Bovine fatalities in the U.S. Confirmed by Small Subunit Ribosomal RNA Gene Analysis of Blood and Tick Samples. J. Chae, M. Levy, J. Hunt, J. Schlater, G. Snider, S. Waghela, P. Holman and G. Wagner. J. Clin. Micro., 37:3037-3040 (1999).

96. Sequence Analysis of the Knockdown Resistance-Homologous Region of the Para-Type Sodium Channel Gene from Pryethroid-Resistant Boophilus microplus (Acari: Ixodidae). H. He, A. Chen, R. Davey, W. Ivie, G. Wagner and J. George. J. Med. Ent., 36:539-543 (1999).

97. Two Theileria cervi ssu rRNA Gene Sequence Types Found in Isolates from White-tailed Deer (Odocoileus virginianus) and North American Elk (Cervus elaphus canadensis). J. Chae, S. Waghela, T. Craig, A. Kocan, Y. Robinson, G. Wagner and P. Holman. J. Wildlife Dis., 35:458-465 (1999).

98. Management Factors Associated with Babesia bovis Seroprevalence in Cattle from E. Yucatan, Mexico. J. Solorio, R. Rodriguez, E. Perez and G. Wagner. Prev. Vet. Med., 40:261-269 (1999).

29

Bibliography -- Scientific Publications in Refereed Journals (continued): 99. A Study of the Systematics of Theileria spp. Based upon Small Subunit Ribosomal RNA Gene

Sequences. J.S. Chae, B.A. Allsopp, S.D. Waghela, J. Park, T. Kakuda, C. Sugimoto, M.T.E.P. Allsopp, G.G. Wagner and P.J. Holman. Parasitol. Res., 85:877-883 (1999).

100. Sequence Analysis of the Knockdown Resistance-Homologous Region of the Para-type Sodium Channel Gene from Pyrethroid-Resistant Boophilus microplus (Acari-Ixodidae). H. He, A. Chen, R. Davey, W. Ivie, G. Wagner and J. George. J. Med. Entomol. 36:539-543 (1999).

101. Cloning and Sequencing of a Putative Acetylcholinesterase cDNA from Boophilus microplus (Acari: Ixodidae). R. Hernandez, H. He, R. Rosario, A. Chen, W. Ivie, J. George, and G. Wagner. J. Med. Ent. 36:764-770 (1999).

102. A Common High Molecular Weight Antigen of Babesia bovis Isolates from Mexico. A. Sahagun, S. Waghela, P. Holman, D. Melendy, and G. Wagner. Int. J. Parasitol., 30:59-64 (2000).

103. Deteccion de B Esterases en Culex quinquefasciatus (Say), Boophilus microplus (canestrini), Amblyomma cajennense (Fabricius), and Blatella germanica (L) por Medio de Zimogramas. M.S. Villarino, S.D. Waghela and G.G. Wagner. Veterinaria Mexico, 31:107-111 (2000).

104. Antigenic, Phenotypic and Molecular Characterization Confirms Babesia odocoilei Isolated From Three Cervids. P. Holman, J. Madeley, T. Craig, B. Allsopp, M. Allsopp, K. Petrini, S. Waghela, and G. Wagner. J. Wildlife Dis., 36:518-530 (2000).

105. Identification of a Point Mutation in an Esterase Gene in Different Populations of the Southern Cattle Tick, Boophilus microplus. R. Hernandez, H. He, A. Chen, S. Waghela, W. Ivie, J. George, and G. Wagner. Insect Biochem. & Mol. Biol. 30:969-977 (2000).

106. Antigenic Analysis of A. marginale Grown in Bovine Erythrocytes Co-cultured with Bovine Endothelial Cells. S.Waghela, D.Melendy, D.Cruz and G.Wagner. Vet. Parasitol. 94:133-139 (2000).

107. Histochemical Localization of Detoxifying Esterases in the Cattle Fever Tick, Boophilus microplus. M. Villarino, S. Waghela, J. George and G. Wagner. J. Med. Entomol. 38:780-782 (2001).

108. Basal Cellular Alterations of Esterase, Glutathione, Glutathione S-Transferase, Intracellular Calcium and Membrane Potentials in Coumaphos Resistant Boophilus microplus (Acari: Ixodidae) Cell Lines. R. Cossio-Bayugar, R. Barhoumi, R. Burghardt, G. Wagner, and P. Holman. Pest. Biochem. Physiol. 72:1-9 (2002).

109. A Cathepsin L-like Cysteine Protease is Conserved among Babesia equi Isolates. P. Holman, M. Hsieh, J. Nix, K. Bendele, G. Wagner, and J. Ball. Mol. Biochem. Parasitol. 119:295-300 (2002).

110. In vitro generation of organophosphate resistant Boophilus microplus (Acari:Ixodidae) cell lines. R. Cossio-Bayugar, G. Wagner and P. Holman. J. Med Entomol. 39: 278-284 (2002).

111. Genotypically Unique Babesia spp. Isolated from Reindeer (Rangifer tarandus) in the U.S. P. Holman, P. Swift, R. Frey, J. Bennet, D. Cruz and G.Wagner. Parasitol. Res. 88:405-411 (2002). 112. Allele Frequency and Gene Expression of a Putative Carboxylesterase-encoding Gene in a

Pyrethroid Resistant Strain of the Tick Boophilus microplus. R. Hernandez, F. Guerrero, J. George and G. Wagner. Insect Biochem. Mol. Biochem. 32:1009-1016 (2002).

113. Evaluation of an Enzyme-Linked Immunosorbant Assay with Recombinant Rhoptry-Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle. S. Boonchit, X. Xuan, N. Yokoyama, W. Goff, G. Wagner and I. Igarashi. J. Clin. Microbiol. 40:3771-3775 (2002).

114. Internationalization of Veterinary Medicine - Educational Opportunities. C. Brown, D. Hird, J. Roth, G. Wagner, C. Jost, and M. Memon. J. Vet. Med. Ed. 29:202-209 (2002).

115. Biochemical Detection of Esterases in the Adult Female Integument of Organophosphate-Resistant Boophilus microplus. M. Villarino, S. Waghela and G. Wagner. J. Med Entomol. 40:52-57 (2003).

116. In vitro Detection of Acaricide resistance in Boophilus microplus (Acari:Ixodidae). M. Villarino, G. Wagner and J. George. Exp. Appl. Acarol. 28:265-271 (2003).

30

Bibliography -- Scientific Publications in Refereed Journals (continued): 117. Babesia odocoilei infection in elk. L. Gallatin, A. Irizarry-Rivira, M. Renninger, P. Holman, G.

Wagner, J. Sojka, and J. Christian. J. Amer. Vet. Assoc. 223:1027-1032 (2003). 118. An initial survey of the cattle grub Dermatobia hominis (L.Jr.) in Nicaragua by Villarino, M.A.,

Garcia, O., Fussell, W., Preston, K. and Wagner, G. Prev. Vet. Med. 61:333-338 (2003). 119. Improved Enzyme-Linked Immunosorbent Assay Using C-terminal Truncated Recombinant

Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies. S. Boonchit, X. Xuan, N. Yokoyama, W. Goff, S. Waghela, G. Wagner and I. Igarashi. J. Clin. Microbiol. 42:1601-1604 (2004).

120. The effect of management factors on the seroprevalence of Anaplasma marginale in Bos indicus cattle in the Mexican tropics. R. Rodriguez-Vivas, Y. Mata-Mendez, E. Perez-Gutierrez and G. Wagner. Trop. Anim. Hlth. Prod. 36:135-143 (2004).

121. Characterization of recombinant antibodies developed for capturing Escherichia coli O157:H7. R. Kanitpun, G. Wagner and S. Waghela. SouthEast Asian J. Trop. Med. Pub. Hlth. 35:902-912 (2004).

122. A single-chain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein. S. Bhaskaran, C. Jay, L. Berghman, G. Wagner, and S. Waghela. Vet. Res. Comm., 29:463-476 (2005).

123. The loss of hemagglutination activity of the enterotoxigenic Escherichia coli K99 pili following treatment with enzymes or chemicals that destroy the conformational structure. C. Jay, S. Bhaskaran, G. Wagner and S. Waghela. JAVA, 4:83-89 (2005).

124. Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbant assay. S. Boonchit, A. Alhassan, B. Chan, X. Xuan, N. Yokohama, M. Ooshiro, W. Goff, S. Waghela, G. Wagner, and I. Igarashi. Vet.Parasitol. 137:28-35 (2006).

125. In vitro cultivation of field isolates of Babesia bigemina for the preparation of immunogens. R. Rodriguez-Vivas, F. Quinones-Avila, G. Ramirez-Cruz, D. Cruz and G. Wagner. Int. J Trop Biol., 55:127-133 (2007).

126. Cysteine protease activity identified in crude extracts of Babesia equi. M. Hsieh, S. Waghela, P. Holman and G. Wagner. J. Clin. Micro. Accepted for publication with revision.

127. Evaluation of financial impact of natural infestations of the cattle tick Boophilus microplus (Canestrini) in two beef and dual purpose cattle ranches in Mexico. M. Villarino, R. Ochoa, S. Waghela, J. George and G. Wagner. J.Econ. Entomol. Submitted.

128. Critical Parameters for modeling the spread of FMD in white-tailed deer populations. Highfield, L., Norby, B., Wagner, G., and Ward, M. Epidemiology and Infection 138:125-138 (2010).

129. A new duplex real-time PCR method for the diagnosis of Mycoplasms gallisepticum and Mycoplasma synoviae. E. Erol, S. Waghela, S.H. Kleven, J. Linares, W. Wigle, S. Wanhooser, G. Wagner, and L. Sneed. Submitted

Book Chapters: Anti-lymphocyte antibody response in cattle inoculated with Theileria parva infected lymphoblastoid cell lines. G.G. Wagner and W.P.H. Duffus. In Parasitic Zoonoses, Clinical and Experimental Studies. Ed. E.J.L. Soulsby, Academic Press, New York, 1974. pp. 97-107. Alterations in complement during acute bovine babesiosis. G. Adams* and G. Wagner. In: The Ruminant Immune System (Adv in Exp.Med. and Biol.Vol.137). Ed. J.Butler, Plenum Press, New York, 1980. pp.793-797. Babesiosis and Heartwater: Threats Without Boundaries. G. Wagner, P. Holman and S. Waghela. In: Veterinary Clinics of North America, (Vet. Clin. Food Anim. Vol. 18) Ed. J. England. Elsevier Science, Philadelphia, 2002. pp. 389-399.

31

Non-Refereed Publications: 1. Prevention of brucellosis in beef cattle. L.G. Adams, R.P. Crawford, D.S. Davis, F.C. Heck, G.G.

Wagner, J.D. Williams and D.L. Zink. In Beef Cattle Research in Texas, PR-3804, 125-127 (1981). 2. Anaplasmosis and babesiosis in Nuevo Leon. S. Romo, R.F. Teclaw and G.G. Wagner. In Bulletin

of the Faculty of Veterinary Medicine and Husbandry (FMVZ), Autonomous University of Neuvo Laredo., Mexico. 1:4-8 (1984)

3. Whipping anaplasmosis isn’t easy - A complicated problem. In Progressive Farmer 108:78 (1993). 4. Un grave problema para los ganaderos. Enfermadades transmitidas por garrapatas. In Beefmaster

Mar-Apr, 1997. 5. Threat of bioterrorism never greater. In Texas Farmer-Stockman, Nov. 2000. 6. One more frightening possibility: Terrorism in the croplands. In The Chronicle of Higher Education. October 26, 2001. 7. Monitoring disease outbreaks is a critical role. In Veterinary Economics, Nov. 2001, p. 16. 8. Americans focus on safer food supply - A report on an International Symposium. In Pig Progress, Vol. 17, No. 6 (2001). 9. Results of a Classical Swine Fever Pathway Analysis – A report of the USDA/APHIS Classical

Swine Fever Pathways Analysis Project. L.K. Holmstrom, H.L. Engleking, H.M. Scott and G.G. Wagner. USDA, APHIS, CEAH. October, 2004.

10. Pathway Analysis of Classical Swine Fever (SCF) Risk to the United States. A Report to the National Agricultural Biosecurity Center Consortium, USDA, APHIS. Washington, DC, October, 2004.

Service Activities: Clinical Service: Preparation and certification of fluorescent antibody test antigens for the serological diagnosis of bovine and equine babesiosis for the USDA, National Veterinary Services Lab., Ames, IA (ongoing). Consultation with occasional cases (hemoparasitic diseases) with the Texas Veterinary Medical Diagnostic Laboratory, practicing veterinarians, various State laboratories, and the USDA. Member, TVMDL Emergency Animal Disease Task Force. USDA, APHIS expert on Foreign Animal and Poultry Diseases. Consultation in the event of a transboundary incursion or suspected introduction of a foreign animal or poultry disease. Laboratory diagnostic services for hemoparasitic infections of food animals and wildlife; samples submitted from private practitioners and state diagnostic laboratories, as well as the states and provinces of Mexico and Canada that border the U.S.