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Genomics-assisted approaches for sustainable
intensification of cereal productivity
Roberto Tuberosa
Dept of Agricultural Sciences University of Bologna Italy
Conference on Scientific Support to Agriculture
competitiveness quality and sustainability
23 April 2014 Athens Greece
a a
Abiotic
stress
Nutrients
Salinity
Metals
Anoxia
Drought
Cold
Heat
Ozone
Multiple stress interaction
a a
Nematodes
Bacteria
Weeds
Virus
Fungi
Insects
Biotic
stress
Empirical breeding has allowed slow but steady
progress in improving yield under a broad range of
environments
Analytical breeding relies on the selection of
morpho-physiological traits (eg canopy temperature
as a proxy for plant water status)
Genomics-assisted breeding and genetic engineering
allow us to dissect and more accurately manipulate
the genetic and functional basis of yield
Breeding strategies for improving crop productivity
Genomics approaches
Forward genetics
Reverse genetics Phenotype
hellipGTACGTAAAThellip
hellipGTACATAAAThellip
Sequence
GeneQTL mapping amp cloning
mutagenesis
Candidate genes genetic
engineering TILLING etc
Reynolds and Tuberosa (2008) Translational research in drought Current Opinion Plant Biology 11171ndash179
Conceptual model for traits associated with adaptation to drought-prone environments grouped
according to main drivers of yield under drought as defined by Passioura (1977)
The QTL approach the crossroad where genetics bioinformatics
agronomy physiology and breeding meet
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
a a
Abiotic
stress
Nutrients
Salinity
Metals
Anoxia
Drought
Cold
Heat
Ozone
Multiple stress interaction
a a
Nematodes
Bacteria
Weeds
Virus
Fungi
Insects
Biotic
stress
Empirical breeding has allowed slow but steady
progress in improving yield under a broad range of
environments
Analytical breeding relies on the selection of
morpho-physiological traits (eg canopy temperature
as a proxy for plant water status)
Genomics-assisted breeding and genetic engineering
allow us to dissect and more accurately manipulate
the genetic and functional basis of yield
Breeding strategies for improving crop productivity
Genomics approaches
Forward genetics
Reverse genetics Phenotype
hellipGTACGTAAAThellip
hellipGTACATAAAThellip
Sequence
GeneQTL mapping amp cloning
mutagenesis
Candidate genes genetic
engineering TILLING etc
Reynolds and Tuberosa (2008) Translational research in drought Current Opinion Plant Biology 11171ndash179
Conceptual model for traits associated with adaptation to drought-prone environments grouped
according to main drivers of yield under drought as defined by Passioura (1977)
The QTL approach the crossroad where genetics bioinformatics
agronomy physiology and breeding meet
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Empirical breeding has allowed slow but steady
progress in improving yield under a broad range of
environments
Analytical breeding relies on the selection of
morpho-physiological traits (eg canopy temperature
as a proxy for plant water status)
Genomics-assisted breeding and genetic engineering
allow us to dissect and more accurately manipulate
the genetic and functional basis of yield
Breeding strategies for improving crop productivity
Genomics approaches
Forward genetics
Reverse genetics Phenotype
hellipGTACGTAAAThellip
hellipGTACATAAAThellip
Sequence
GeneQTL mapping amp cloning
mutagenesis
Candidate genes genetic
engineering TILLING etc
Reynolds and Tuberosa (2008) Translational research in drought Current Opinion Plant Biology 11171ndash179
Conceptual model for traits associated with adaptation to drought-prone environments grouped
according to main drivers of yield under drought as defined by Passioura (1977)
The QTL approach the crossroad where genetics bioinformatics
agronomy physiology and breeding meet
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Genomics approaches
Forward genetics
Reverse genetics Phenotype
hellipGTACGTAAAThellip
hellipGTACATAAAThellip
Sequence
GeneQTL mapping amp cloning
mutagenesis
Candidate genes genetic
engineering TILLING etc
Reynolds and Tuberosa (2008) Translational research in drought Current Opinion Plant Biology 11171ndash179
Conceptual model for traits associated with adaptation to drought-prone environments grouped
according to main drivers of yield under drought as defined by Passioura (1977)
The QTL approach the crossroad where genetics bioinformatics
agronomy physiology and breeding meet
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Reynolds and Tuberosa (2008) Translational research in drought Current Opinion Plant Biology 11171ndash179
Conceptual model for traits associated with adaptation to drought-prone environments grouped
according to main drivers of yield under drought as defined by Passioura (1977)
The QTL approach the crossroad where genetics bioinformatics
agronomy physiology and breeding meet
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
The QTL approach the crossroad where genetics bioinformatics
agronomy physiology and breeding meet
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
QTLs for drought resistance
one size doesnrsquot fit all
A given QTL allele can have positive null or negative effects
depending on the drought environment This complication has
slowed considerably the utilization of QTL data for breeding
Collins Tardieu Tuberosa (2008) Plant Physiology 147 469-486
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
GeneQTL
discovery
GeneQTL characterization
- Genotype x Environment x Manage
- Validation in different genetic
backgrounds
Marker-assisted breeding
- Cost-effectiveness
- High-throughput profiling
GeneQTL
cloning
Perfect marker
TILLING EcoTILLING
genetic engineering
Deploying the genomics pipeline
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
To clone or not to clone QTLs
Salvi amp Tuberosa (2005) Trends in Plant Science 10 297-304
Cloning QTLs as an essential step to
bull Unravel the functional basis of agronomic traits
bull Unlock the allelic richness of germplasm by
direct haplotyping and sequencing of target loci
bull Identify the perfect marker for selection
bull Apply genetic engineering
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Chasing major QTLs
for grain yield and yield stability
in durum wheat
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Durum Panel
(Maccaferri et al 2005)
260 accessions
(elite cultivars)
Chosen based on pedigree and
phenology (heading date)
From Italy Spain Morocco
Tunisia Southern USA
CIMMYT and ICARDA
350 SSR
900 DArT
29000 SNP
Kofa x Svevo
249 RILs
Colosseo x Lloyd
176 RILs
(Neodur x Claudio)
X
(Colosseo x Rascon)
330 RILs
Linkage mapping
RIL
populations
Association mapping
Elite lines
collection
Traits
Resistance to
virus
leaf amp stem rust
Fusarium
Septoria
Drought
resistance
Grain yield
Yield stability
QTL
mapping
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
IDuWUE Improving Durum wheat for Water-Use Efficiency and yield
stability Project funded by the EU
Partners Italy (2) Spain (3) Morocco Tunisia Lebanon Syria ICARDA
Objective
bull Identify QTLs affecting yield WUE and related traits in durum wheat
grown across environments with a broad range of water availability
Approach
bull Linkage mapping 249 RILs (Kofa x Svevo)
bull 16 field trials with a 10-fold range in yield (06 ndash 59 tha)
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Trait Number of QTLs significant in
1 env 2 env 3 or more env
Grain yield 15 1 2
Heading date 7 2 4
Plant height 5 1 6
Grain weight 7 3 3
Grainsm2 13 4 3
Summary of significant QTLs (LOD gt 25)
Adaptive Constitutive
Q T L e f f e c t
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Effects of the chr 2BL and 3BS QTLs on grain yield
in 249 RILs (Kofa x Svevo) tested in 16 environments
Chrom arm QTL Environ R2
(no) ()
2BL QYldidw-2B 8 215
3BS QYldidw-3B 7 138
2BL x 3BS 6 140
Grain weight
2BL QTgwidw-2B 8 112
3BS QTgwidw-3B 8 130
2BL x 3BS 7 156
Maccaferri et al (2008) Genetics 178 489-511
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
0
10
20
30
40
50
60
70
80
90
10
0
11
0
12
0
53
83
4
62
7
40
84
24
37
80
13
58
15
66
NIL (++)
cM
00
0
17
8
27
4
33
4
33
9
34
5
35
6
36
6
40
2
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
7
42
9
43
2
43
2
43
2
43
2
44
3
46
5
49
0
49
2
52
8
61
0
62
4
64
8
Lo
cu
s
cft5055
barc
133
cfb
011
ubw
3B
50
ubw
3B
51
ubw
3B
52
ubw
3B
53
cfb
6127
ubw
3B
54
ubw
3B
55
cfb
6149
cfb
6021
cfb
6134
cfb
6133
cfb
6148
ubw
3B
58
ubw
3B
59
cfb
6107
cfb
6142
cfb
6104
Phenoty
pe L
co
ubw
3B
60
cfb
6034
cfb
6033
cfb
6022
cfb
6032
cfb
6016
cfp
60
cs-s
sr7
wm
s493
SE
GM
EN
TA
L I
SO
LIN
ES
53 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
72 a a a b b b b b b b b b b b b b b b b b b b b b b b b b b b
83 a a a a b b b b b b b b b b b b b b b b b b b b b b b b b b
34 a a a a a b b b b b b b b b b b b b b b b b b b b b b b b b
4 a a a a a a b b b b b b b b b b b b b b b b b b b b b b b b
12 a a a a a a a b b b b b b b b b b b b b b b b b b b b b b b
62 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
256 a a a a a a a a b b b b b b b b b b b b b b b b b b b b b b
7 a a a a a a a a a b b b b b b b b b b b b b b b b b b b b b
69 a a a a a a a a a a a a a a a a a b b b b b b b b b b b b b
40 a a a a a a a a a a a a a a a a a a a a a a b b b b b b b b
21 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
84 a a a a a a a a a a a a a a a a a a a a a a a a b b b b b b
50 a a a a a a a a a a a a a a a a a a a a a a a a a a a a b b
24 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
39 b b b b b b a a a a a a a a a a a a a a a a a a a a a a a a
37 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
41 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
80 b b b b b b b b a a a a a a a a a a a a a a a a a a a a a a
51 b b b b b b b b b a a a a a a a a a a a a a a a a a a a a a
13 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
56 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
58 b b b b b b b b b b b b b b b b b b b b b b b b a a a a a a
60 b b b b b b b b b b b b b b b b b b b b b b b b b a a a a a
15 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
55 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
66 b b b b b b b b b b b b b b b b b b b b b b b b b b a a a a
NIL (--) b b b b b b b b b b b b b b b b b b b b b b b b b b b b b b
NIL (++) a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a
Grain yield
q h
a-1
648 t ha-1
888 t ha-1
693 t ha-1
QYldidw3B
Mendelizing QYldidw-3B
a = 111 t ha-1
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Mapping QTLs for root system architecture
in durum wheat
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Phenotyping the AM panel with semi-hydroponics protocol
Seminal Root Angle (deg) Project 244374 DROPS
Partner 12 UniBO
Project 289300 EURoot
Partner 9 UniBO
DP034 DP045
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Chasing major QTLs for
phenology and root architecture
in maize
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
B73 Gaspeacute Flint F1
20-d
ay d
iffe
ren
ce
7 days
Vgt1
13 days
other
major
loci
Flowering time in B73 and Gaspeacute Flint
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
N28
Early N28
N28E N28
NILs for the Vegetative to generative transition 1 (Vgt1) locus
Salvi et al (2007) Proc Nat Acad Sci 104 11376
Gaspeacute Flint
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
bull Vgt1 corresponds to a 2 kb noncoding sequence (Salvi et al 2007)
bull Vgt1 regulates the expression of ZmRap27 a repressor of
flowering of the Ap2 family
The Vegetative to Generative Transition 1 locus
MITE
(143 bp)
Vgt1 (1973 bp)
~ 70 kb
ZmRap27
bull A MITE transposon insertion is associated with differential
methylation at the maize flowering time QTL Vgt1
(Castelletti et al 2014 G3 doi101534g3114010686)
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
BB
Os (--) Os (++) 120 (--) 129 (++)
803
1973
474
1024
00
500
1000
1500
2000
2500
120 (--) 129 (++) OS-- OS++
root-yield-106 root-ABA1-204
Contrasting NILs for root architecture QTLs in maize
For yield see Landi et al (2010 J Exp Bot) For yield see Landi et al (2007 J Exp Bot)
Ro
ot
dry
wei
gh
t
Martinez et al
(unpublished) Giuliani et al
(unpublished)
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Where is the beef
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Genomics-assisted breeding
Maize
- Artesian (2010 Syngenta)
- AQUAmax (2011 Pioneer)
Rice
- PY84 or Birsa Vikas Dhan (2012 India)
Genetic engineering
Maize
- DroughtGard (2013 Monsanto)
Releases of drought-tolerant cvs via biotec-based approaches
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Publication number US20120317678 A1
Publication type Application
Application number US 13517215
Publication date Dec 13 2012
Also published as CA2782300A1
Inventors Yusaku Uga
Gene Dro1 Controlling Deep-Rooted
Characteristics of Plant and
Utilization of Same
httpwwwgooglecompatentsUS20120317678
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
QTL-based improvement for
yield and yield stability
Perspectives and
future challenges
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Past
Genotyping
Phenotyping
Future
Genotyping
Phenotyping
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
DROught-tolerant yielding PlantS
wwwdrops-projecteu
Coordinator Francois Tardieu INRA-LEPSE 34060 Montpellier France
15 Partners Allelic diversity
(panels of lines mapping)
Association with phenotypes (field and controlled platforms)
Crop modelling (prediction under drought
scenarios)
Response to water deficit through
seed abortion
leaf growth
root architectur
e
water-use efficiency
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Future research
priorities
Better understanding of
- Root functions and plasticity
- Reproductive failure under drought stress
- Interactions of abiotic and biotic stresses
- Genetic functional basis and modeling of yield
- Role of epigenetics in G x E
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
- High-throughput relevant phenotyping
- Cloning of major QTLs for adaptive traits and yield
- Mining wild germplasm for novel favourable alleles
- Interdisciplinary training and capacity building
- Strong Public-Private Partnerships (PPPs)
-
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Massi et al Produttori Sementi Bologna Feuillet et al INRA France
Taramino et al Pioneer Dupont USA Ouzunova et al KWS Germany
Many thanks to
bull Maria Angela Canersquo
bull Sara Castelletti
bull Chiara Colalongo
bull Simona Corneti
bull Walid Elfeki
bull Nazemi Ghasemali
bull Silvia Giuliani
bull Marta Graziani
bull Pierangelo Landi
bull Marco Maccaferri
bull Paola Mantovani
bull Ana Martinez
bull Sara Milner
bull Andrea Ricci
bull Silvio Salvi
bull Maria C Sanguineti
bull Josersquo Soriano
bull Sandra Stefanelli
bull Valentina Talamersquo
Funding European Union Produttori Sementi Bologna Pioneer-DuPont KWS
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
Feeding the Planet Energy for Life
May-October 2015 Milano Italy
