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GENERAL EMBRYOLOGY1. Development of embryology2. Germ cell and fertilization3. Blastocyst and implantation4. Formation of the germ layer5. Differentiation of trilaminar germ and
formation of embryo6. Fetal membrane and placenta7. Twins and multiple birth
Preembryonic period: 1st week to end of 2nd week
fertilization to formation of bilaminar germ disc
Embryonic period: 3rd week to end of 8th week formation of embryo
Fetal period: 9th week to birth growth
Perinatal stage: 26th week to 4 week after birth
Human ?
Plant
Animal
Development of embryology
Semen+menstration
Harvey, 1651: All life from oocyte
Malpighi, 1675: A micro-chicken in an egg
Leewenhoek, 1677 : A micro-human in a sperm
Aristotle, B.C 384-322
Haeckel, 1868 Biogenetic law
Spemann, 1869-1941
Experimental embryology
Wilmut,1997 Dolly
Germ cell and fertilization
Germ cell spermatozoon and ovum
1. Spermatozoon
Capacitation : In female reproductive tract, the sperms were enabled to bind to the zona pellucida receptors.
removal of glycoproteins (decapacitation factor) which cover the sperms
2. Ovum secondary oocyte arrested at metaphase in the second meiotic division
Fertilization: The process in which the spermatozoon penetrates into the ovum to form fertilized ovum. In the ampulla of oviduct
nucleus acrosome
Penetration and release of acrosomal enzyme
Cell membrane of ovum
Process of fertilizationSperm bind to sperm receptor ZP-3 induceAcrosome reaction : release of acrosomal enzyme
ovum
fertilized ovum
sperm
The second meiotic division of the secondary oocyte is rapidly lifted and the second polar body is released, leaving a haploid female nucleus.
Zona reaction: Cortical granules→perivitelline space →degrade ZP-3, alteration of zona pellucida →barrier for sperm penetration
Contac of sperm and ovum
Sperm penetrate into the ovum
Formation of female and male pronucleus
Fusion of pronuclei
① Fusion of the membrane of
sperm and ovum
② The nucleus of sperm penetrate into ovum
③ Formation of pronuclei
④ Fusion of
pronuclei
Process of fertilization
Alteration of ovum 24 h
2.Condition of fertilization ① Normal ovum
② Normal sperm
sufficient numbers
③ Certain time
12-24h
④ Free reproductive tract
3.Significance of fertilization ① A new life
② Diploid
inheritance and aberrance
③ Sex determination
Formation of blastocyst and implantation
Cleavage and formation of blastocyst
1. Cleavage : early division of fertilized egg
2. Blastomere : daughter cells from cleavage
3. Morula : 12 to 16- cell stage, enclosed in the zona pellucida,
like morus
4. Blastocyst : about 100 blastmeres blastocoele 、 inner cell mass and trophoblast
polar trophoblast
Phase of cleavage
Fertilized egg
2-cell stage
18~36h
4-cell stage
36~48h
8-cell stage
48~60h
Morula
12 to 16-cell stage
The third day
Appearance and inner structure of blastocyst
Appearance Inner structure
Movement of fertilized egg in the oviduct
Implantation (imbed) The process by which the blastocyst settles into endometrium. 6th day to 11th day Fundus and body of uterus
zona pellucida disappear at the 5th day1.Trophoblast : proliferate and differentiate into two layers①Syncytiotrophoblast : outer layer, fused each other, cell
boundaries disappear②Cytotrophoblast : inner layer , cell boundaries distinct,
simple cuboidal cells
Movement and implantation of fertilized egg
Implantation at 7th day
Endometrium
Polar trophoblast
Inner cell mass
Cytotrophoblast
2.Decidua : endometrium implantation decidua decidual response Decidua basalis : under the implantation site Decidua capsularis : between the implantation site and the uterine lumen Decidua parietalis : remaining endometrium
Decidua
Formation of germ layer
Bilaminar germ disc
1.Bilaminar germ disk : Inner cell mass→2 layers of cell in disc (7th day)
Epiblast: columnar cells adjacent to trophoblast
Hypoblast: cuboidal cells adjacent to blastocoele
Bilaminar germ disc
Epiblast
Hypoblast
Columnar cells
Cuboidal cells
Epiblast
Hypoblast
2. Amnion : 8th day
epiblast → amnioblast → aminiotic membrane → amniotic cavity → amniotic fluid
3.Primary yolk sac : 9th day
hypoblast →extraembyronic endoderm →primary yolk sac
Bilaminar germ disc at 9th day
Hypoblast
Epiblast
Amniotic cavity
Syncytio-trophoblast
Primary yolk sac
Bilaminar germ disc
Decidua
Syncytiotrophoblast
Epiblast
HypoblastPrimary yolk sac
Amniotic cavity
Cytotrophoblast
4. Extraembryonic mesoderm: 10th-11th day
5. Extraembryonic cavity: 12th-13th day Extraembryonic mesoderm: visceral layer
parietal layer
6. Secondary yolk sac: 13th day
7. Body stalk: 14th day
Embryo at 10th dayPrimary yolk sac
Extraembryonic mesoderm
Germ disc
Amnion
Bilaminar germ disc
Decidua
Amniotic cavityEpiblast
Hypoblast
Extraembryonic mesoderm
Primaryyolk sac
Cytotrophoblast
Syncytiotrophoblast
Embryo at 13th day
Extraembryonic cavity
Primary yolk sac
Secondary yolk sac
Body stalk
Bilaminar germ disc
Body stalk
Secondary yolk sac
Extraembryonic cavity
Primary stem villus
Extraembryonic mesodermVisceral layer
Extraembryonic mesoderm
Parietal layer
Trilaminar germ disc
primitive streak, primitive groove 3rd week
primitive node, primitive pit 3rd week
1. Endoderm: primitive groove →hypoblast → endoderm
2. Mesoderm : primitive groove →between epiblast and hypoblat →mesoderm
3. Ectoderm : epiblast →ectoderm
Formation of trilaminar germ
Primitive streak
Epiblast
Hypoblast
Epiblast
Hypoblast
Primitive streak
Determine the
direction of the
embryo
notochord
Primitive node
Primitive
pit
Primitivegroove
Primitivestreak
Significance of
primitive streak
Hypoblast
Epiblast
Primitive
streak
Endoderm
Formation of endoderm
Epiblast
Endoderm
Formation of mesoderm
Mesoderm
Ectoderm
Endoderm
Mesoderm
Trilaminar germ disc
Epiblastproliferation
Primitive pit
Headprocess
Notochordal tube
Neurenteric canal
Notochord
Significance of notochord ?
No mesoderm
Buccopharyngeal membrane
Cloacal membrane
Differentiation of trilaminar germ and formation of embryo
Differentiation of trilaminar germ1.Differentiation of ectoderm
①Neural tube : blastema of CNS
neural plate (18th-19th day) Neuro-epithelium (neural ectoderm): pseudostratified columnar
→neural groove , neural fold
→ neural tube (closed at 22nd day)
Neural groove Paraxial mesoderm Intermediate mesoderm
Neural groove
Paraxial mesoderm intermediate mesoderm
Neural groove
Neural crest
Notocord
1 、 Differentiation of ectoderm
Anterior neuropore closed at 25th
Posterior neuropore closed at 27th
Neural tube
Neural fold
closed at 22nd day, from 4th somite
Unclosed Anterior neuropore Unclosed Posterior neuropore
②Neural crest : blastema of PNS
chromaffin cell, parafollicular cell
some of bone, cartilage and
muscle of head
③Superficial ectoderm: after formation of neural tube
epidermis
2.Differentiation of mesoderm 17th day
①Paraxial mesoderm : somite at 20th day, from the head
Dermotome→ dermis, hypodermis
Myotome → skeletal muscle,
Sclerotome → axial skeleton.
②Intermediate mesoderm : urinary and
reproductive system
③Lateral mesoderm : parietal mesoderm , viseral mesoderm , intraembryonic coelomic cavity
Somite
Parietal mesoderm Intraembryonic
coelomic cavit
Visceral mesoderm
Differentiation of lateral mesoderm
Dermatome
Sclerotome
Extraembryonic coelomic cavity
Intraembryonic coelomic cavity
Differentiation of lateral mesoderm
3. Differentiation of endoderm
Epithelium of primitive gut →
epithelium of digestive tract, digestive gland,
respiratory tract and lung
Primitive pharynx →5 pairs of pharyngeal pouch
Formation of primitive gut
A B
Parietal mesoderm
Intraembryonic coelomic cavity
Visceral mesoderm
Formation of embryonic body (4-8th week)
Disc Column folds
the number of somite increase
face development, formation of branchial pouch
breach of buccopharyngeal membrane
5th week: upper and lower limb buds
6th week: hand and foot plates
7th week: formation of face, disappear of somite
8th week: breach of cloacal membrane
20-30th day
At the end of 8th week—
a small human
Fetal membrane and placenta
Fetal membrane
Chorion Amnion Yolk sac Allantois Umbilical cord
Amnion
Yolk sac
Umbilical cord
Placenta
1.Chorion : Secondary stem villus +chorinic plate
Primary stem villus : 2nd week
Outer syncytiotrophoblast+ Inner cytotrophoblast
Chorinic plate : extraembryonic mesoderm + trophoblast
Secondary stem villus : 3rd week
Outer syncytiotrophoblast+
Intermedial extraembryonic mesoderm+
Inner cytotrophoblast
Tertiary stem villus : end of 3rd week, Blood vessels
Anchoring villus, Free villus cytotrophoblastic cell column → cytotrophoblastic shell
Primary stem villus : at 2nd week , cytotrophoblast
Secondary stem villus : at 3rd week , extraembryonic mesoderm Tertiary stem villus : at the end of 3rd week , blood
vessels
Anchoring villus
Free villus
Cytotrophoblastic shell
Cytotrophoblastic column
Stem villus
Evolvement of chorion
Chorion laeve Chorion frondosum
6th weeks
Chorion laeve : adjacent to decidua capsularisChorion frondosum : adjacent to decidua basalis
Abnormility of chorion :
Hydatidiform
Hydatidiform
• Chorion carcinoma
• Chorion carcinoma
Picture
Hydatidiform
Hydatidiform
Hydatidiform
Chorion carcinoma
Chorion carcinoma
Chorion carcinoma
2. Yolk sac : 5th-6th week, atresia
Abnormality: ① Meckel’s diverticulum
② Umbilical fistula
① Primitive blood cells
② Primordial germ cells
3. Amnion : amniotic membrane: 0.2-0.5mm
amniotic epithelium + extraembryonic mesoderm
amniotic fluid: 500-1000ml
abnormality: < 500 ml, oligohydramnios
> 2000 ml, polyhydramnios
protection
4. Allantois : degeneration Abnormality: Urachal fistula
2 pairs of blood vessel→ 1 umbilical vein 1 pair of umbilical artery
5. Umbilical cord : 50 cm
(CT, umbilical vein , umbilical artery,
degenerated yolk sac and allantois)
covered with amniotic membrane Abnormality: > 80 cm,
< 35 cm
connect fetus with placenta
Fetal membrane
Placenta1.Placenta: Chorion frondosum + Decidua basalis
disk: 15-20cm Sandwich: chorinic plate (fetus) villi basal plate (mother) basal plate: decidua + cytotrophoblastic shell
placental septa → cotyledon (incomplete)
Umbilical arteryUmbilical vein
Spiral arteryUterus vein
Decidua bsalisPlacenta septa
2.Placental membrane : Structure between the blood of mother and fetus
①Syncytiotrophoblast ; ②Cytotrophoblast and its basal lamina ; ③Connective tissue ; ④Endothelium and its basal lamina
protection
胎儿血
Syncytiotrophoblast
Cytotrophoblast
Basal lamina
Connective tissue
Basal lamina
Endothelium
Nutriment
Metabolic product
Fetal membrane
Blood of mother
3.Function : ① Substance exchange
O2, nutriment, CO2, waste
② Hormone
Human chorionic gonadotropin, HCG;
Human placental lactogen, HPL;
Human placental progesterone, HPP,
Human placental estrogen, HPE
FunctionFunction
① Substance exchange
① Substance exchange
Placenta
Capillary of fetus Blood of mother
O 2
Nutriment
water 、 salt 、 others water 、 salt 、 others
CO 2
Metabolic waste
Placenta membrane
FunctionFunction
② Barrier
② Barrier
Placenta
Capillary of fetus Blood of mothers
Placenta barrier
No way!
Most of microorganism
FunctionFunction
② Barrier
② Barrier
Placenta
Capillary of fetus Blood of mother
Most of drugs
Placenta barrier
Most of microorganism
Some of microorganism
FunctionFunction
③ Endocrine ③ Endocrine
Placenta
Hormone Function Appearance Fastigium
HCGCorpus luteum
Pregnancy3rd week 8th week
HCSMammary
gland2nd month 8th month
HPE Corpus luteum 4th month 8th month
HPP Corpus luteum 4th month 8th month
Twins and multiple birth
1.Twins monozygotic twins
dizygotic twins
2.Multiple birth monozygotic multiple birth
polyzygotic multiple birth
mixed multiple birth
Dizygotic twins
Blastocyst Blastocyst
2 Amnion 2 Amnion2 Chorion 2 Chorion2 Placenta 2 Placenta
Monozygotic twins
separation of blastomere, of ICM, of primitive streak
Blastocyst 2 ICM 2 primitive streak
2 Amnion 2 Amnion 1 Amnion
2 Chorion 1 Chorion 1 Chorion
2 Placenta 1 Placenta 1 Placenta