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Gene Technology

Gene Technology. Vocabulary—Yay! 1.Polymerase chain reaction 2.Primer 3.Restriction enzyme 4.Gel electrophoresis 5.DNA fingerprint 6.Genetic engineering

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Text of Gene Technology. Vocabulary—Yay! 1.Polymerase chain reaction 2.Primer 3.Restriction enzyme 4.Gel...

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  • Gene Technology
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  • VocabularyYay! 1.Polymerase chain reaction 2.Primer 3.Restriction enzyme 4.Gel electrophoresis 5.DNA fingerprint 6.Genetic engineering 7.Recombinant DNA 8.Clone 9.Vector 10.Plasmid 11.Probe 12.Bioinformatics 13.Proteomics 14.Cloning by nuclear transfer 15.Telomere 16.DNA vaccine 17.Bioethics
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  • Selective Breeding Selective Breeding is the technique of allowing only those animals with desired characteristics to produce the next generation. Can you think of a time when humans actually use selective breeding?? Nearly all domestic animals, crops This can lead to inbreeding and less genetic variety and disorders and early death To increase genetic variation---introduce mutations
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  • The Super Cow Reflect on what you just saw. 3-5 sentences. What do you think? Is it right? Can you see this used in other ways? What are the benefits? Disadvantages?
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  • DNA Identification Except for Identical Twins, no 2 people in the world are exactly alike genetically. The majority of human DNA is the same. Only about.1% of our DNA varies by individual.
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  • Steps in DNA Identification 1.Copying DNA via Polymerase Chain Reaction or PCR a)Often you only have a small amount of DNA (ie crime scene) so you need to copy it b)Video ClipVideo Clip 2.Cutting DNA with restriction enzymes a)To cut long DNA molecules biologists use bacterial proteins or restriction enzymes to cut out certain sequences
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  • Steps in DNA Identification 3. Sorting DNA by size a)DNA fragments can be studied with GEL ELECTROPHORESIS, which separated DNA by size and charge. b)This creates a DNA fingerprint, unique to the individual c)Video ClipVideo Clip 4.Comparing DNA 4.Using the DNA fingerprint scientists can produce the odds that 2 people will have the same DNA fingerprint only about 1 in 100 billion (and there are only 6.5 billion people on Earth).
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  • Recombinant DNA The process of altering genetic material is called GENETIC ENGINEERING. RECOMBINANT DNA results when DNA from 2 different organisms are joined. Example of Recombinant DNAscientists inserted a jellyfish gene (for a fluorescent green glow) into a zebra fish to see blood vessels develop. Video Clip6 facts Video Clip
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  • Cloning GENETIC ENGINEERING has also allowed biologists to clone entire organismsnot just genes. In the 1990s scientists began cloning whole organisms or CLONING BY NUCLEAR TRANSFER. They introduce a nucleus of a body cell into an egg cell to generate an organism identical to the donor. Dolly, a sheep, was the first successful clone in 1996. Video
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  • Stem Cells Brainpop!
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  • Genetic Engineering and Food Genetic Engineering with plants is called genetically modified (GM) crops. GM crops are more tolerant of weather conditions (frost, drought), resistant to herbicides, increase the amount of the crop produced, resistant to disease, improved nutritional value of foods
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  • Genetic Engineering and your Food 1.You dont want to sound like these people!You dont want to sound like these people! 2.GMOs While you watch make a chart of benefits and possible controversies.GMOs 3.ChartBenefits and Controversies 4.Article 5.Opinion Paragraph
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  • Designer Babies Issues and Decisions Worksheet Extra Credit Opportunities!! Watch one or both of the following movies, Trailers!: Gattaca or My Sisters Keeper 2 page, typed, report on the film Report should include a summary of the storyline and how it ties into Genetics and Designer Babies Report also needs to be signed by a parent, verifying that you did indeed watch and have permission to watch the movie
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  • Genetic Science Ethics Survey 1.As a table group, read each scenario aloud. 2.On a separate sheet of paper (each person has their own), Mark your choice. Then mark the groups majority rules decision. 3.Complete the final discussion at the end of the papereach person should write their own paragraph.
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  • Bioethics Group Reports 1.In your table groups read your scenario, read the questions. 2.Read your designated chapters. 3.Answer the questions. 4.Prepare a presentation to the class on your scenario. 1.Explain the scenario. 2.Go over the questions and answers. 3.Provide a visual (pictures, vocab, charts, pros/cons) 5.Present to the class
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  • Genetic Engineering Concept Map Review