Gene Engineering Division - Riken

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Head

Gene Engineering Division

Goal

Activities

The Gene Engineering Division (RIKEN DNA Bank) is a

nonprofit resource archive that provides genetic resources,

technical services and educational program to qualified

investigators in private industries, governments and academic

organizations around the world. RIKEN DNA Bank has been

selected as a central facility for collecting, preserving and

delivering DNAs of animals and microorganisms through

the National BioResource Project sponsored by the Ministry

of Education, Culture, Sports, Science and Technology of

JAPAN (MEXT). Our division conducts research to ensure

1. Collection, preservation and distribution of genetic

resources

The Gene Engineering Division (RIKEN DNA Bank) is

divided into seven sections for DNA Banking.

(1) Cloned-DNA Set Bank. This handles a cloned collection

of full-length cDNAs assembled with specific research

areas such as hormones, cytokines, apoptosis, cell cycle,

signal cascades, transcription factors, replication factors,

and ubiquitination. These representative cloned sets were

isolated from cDNA libraries, phage, cosmid, BAC, YAC,

PAC, P1 and phosmid libraries.

(2) Japanese-Specific DNA Bank. This handles human HLA

class I clones that are unique to Japanese and SEREX

clones coding cancer antigens of Japanese origin and to

HistoryRIKEN DNA Bank was established in June 1987 when a

committee of scientists recognized the need for a central

collection of recombinant DNA that would serve scientists in

Asia. In 2001, RIKEN BioResource Center (RIKEN BRC)

was established and then the DNA Bank was reorganized into

the Division of Gene Engineering. In 2002, the division was

Kazunari K. YOKOYAMA, Ph.D.

the authenticity of the genetic materials in the collection, and

to improve and standardize the methods of characterization,

maintenance, preservation and distribution of genetic

materials. We distribute cloned DNAs, gene libraries

(e.g., cDNAs, phages, cosmids, BAC, phosmids, and YAC

libraries), vectors, hosts, recombinant viruses and ordered

library sets from humans, mice, microorganisms, viruses

and other animals. Our division also performs and sponsors

research to improve and standardizes for the advancement,

validation and application of scientific knowledge.

recognized as the central archive for the collection of “animal

DNA and microorganism DNA” in the National BioResource

Project (NBRP) program (Phase I), sponsored by MEXT. In

2007, it was also recognized as the central archive for the

collection of “DNA” in the NBRP (Phase II), sponsored by

MEXT.

other clones for Japanese heredity.

(3) Recombinant-Virus Bank. This handles recombinant

viruses constructed by inserting a full-length cDNA into

viral vectors, generating the viral particles as resources.

The viruses are examined of their qualities by methods

developed in our division. The DNA fragments derived

from human and mouse full-length cDNA libraries were

used as donors of recombinant viruses.

(4) Promoter Bank. This handles promoter DNA fragments

fused to reporter genes such as luciferase, lacZ, GFP and

Cre recombinase. Transgenic promoter Cre mice are also

generated by collaborating with the Animal Resource

Center of Tsukuba University and the Experimental

Animal Division of RIKEN BRC. The second phase of

Gene Engineering DivisionRIKEN BRC Annual Report 2005 ～ 2007

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MembersHead

Kazunari K. YOKOYAMA, Ph.D. (2001.4~)

Senior Research Scientist

Takehide MURATA, Ph.D. (2001.4~) Jianzhi PAN, Ph.D. (2005.4~)

Makoto KIMURA, Ph.D. (2003.7~2007.9)

Contract Researcher

Koji NAKADE, Ph.D. (2004.1~)

Visiting Scientist

Tetsuo HIRANO, Ph.D. (2001.4~) Michiya NOGUCHI, Ph.D. (2008.5~)

　JRA (~2008.3)

Visiting Technician

Tsuyoshi AOKI (2008.4~)

Technical Staff Ⅱ

Kumiko INABE (2002.4~) Takahito YAMASAKI (2003.4~)

Yukari KUJIME (2004.4~) Megumi HIROSE (2004.4~)

Satoko MASUZAKI (2006.4~) Sanae YAMAOKA (2004.4~2008.3)

Agency Staff

Kazuko UENO (2001.4~)

Carlo Dante T.NATIVIDAD, Ph.D. (2008.4~2008.11)

this Bank is to address the promoters of p53-related genes

with reporter constructs.

(5) Archives of large-genome cloned library. This handles

cDNA and genome libraries from various animals. This

section was based on the NBRP for collecting all genome

resources produced by the NBRP (Phases I and II) such

as those from Xenopus, Japanese monkeys, rats, mice,

humans, yeasts, Ciona and Thermus thermophilus.

(6) Basic domain of DNA Bank. This handles individual

cDNAs, genome DNA clones and vectors as well as host

cells.

(7) Bioinformatics section. This handles the informatics of our

genetic resources for DNA Banking.

We distribute genetic resources to only qualified researchers

associated with certain research, medical or educational

organizations. We also report the activities of our division in

an annual report, qualified by the “Resource Committee”. We

also discuss the future plan of our mission. The “Resource

Ethics Committee” confirms the banking activity of genetic

resources of humans every year. The “Advisory Council”

is held to evaluate the activities of RIKEN BRC every other

year. We evaluate not only the activities of our DNA Banking

but also the research activities of developing technologies

related to DNA Banking.

2. Development of new technology to ensure the

authenticity of genetic materials

The development and improvement of methods for the

standardization and characterization of genetic resources

are also conducted by our division. These technologies,

as described below, are necessary and entail the following:

1) identification of mutation sites in genetic resources; 2)

preparation of novel vectors and an adenovirus vector for

the controlled expression system of genes; 3) preparation of

an artificial reporter vector with different cis-elements and

promoter-reporter constructs; 4) development and validation

of new gene-transferring system using targeted promoter/

enhancer element; 5) development of a new system for

studying gene expression in eukaryotic cells and animals; and

6) production of modified proteins in Escherichia coli on a

large scale.

3. Education and training of scientists.

Our division offers a training program for young scientists and

students that teaches the best use of adenoviral vectors.


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Specific Aims

I. Collection, preparation and distribution of genetic materials.

1. Banking system

We have collected the following numbers of genetic materials:

host, vector, cloned DNA, 3,451; Nakamura-White RELP

marker clone, 123; human genomic YAC clone library,

35,712; human full length cDNA cloned library, 307,200;

mouse 15 K cDNA cloned library, 15,000; mouse 7.4 K

cDNA cloned library, 7,407; mouse cDNA cloned library,

45,216; cDNA library, 47; mouse BAC cloned library,

193,152; human SEREX clone, 584; Japanese HLA class I,

40; recombinant virus, 502; NBRP Japanese macaque genome

library, 200,064; Tokushima Univ. mouse cDNA cloned

library, 374,208; common marmoset cDNA cloned library,

353,664; chimpanzee 22nd chromosome genome library,

13,824; NBRP Xenopus laevis cDNA cloned library, 186,400;

NBRP Xenopus tropicalis cDNA cloned library, 44,544;

cricket cDNA cloned library, 60,288; Thermus thermophilus

HB8 expression/KO plasmids, 2,753; Schizosaccharomyces

pombe cDNA cloned library, (ORFeome library) 14,436;

human genome library, 399,456, and Ciona intestinalis cDNA

cloned library, 452,352.

Operation of automated DNA-extraction machine

Masato OKUBO (2008.4~) Chitose KURIHARA (2008.4~)

Yuri NAKANO (2008.4~) Yuka KUSA (2008.4~)

Miyuki YAMAMOTO (2003.6~2008.4) Reiko OHTO (2007.4~2008.4)

Student Trainee

Rei KAWASHIMA, M.D. (2005.4~) Shinji ENDO (2007.4~)

Atsushi HIGA (2007.5~2008.2) Mariko WAKAYAMA (2002.4~2007.3)

Yusuke IDE (2005.4~2006.2)

Nakade, Arita, Katsuya, Kusa, Inabe, Hirose, Masuzaki, Nakajima, Sato, Kimura, NoguchiKurihara, Kujime, Fujisawa, Takahara, Hattori, Hiraguri, Ueno, Nakano, Furuya

Natividad, Kawashima, Pan, Yokoyama, Murata, Yamasaki, Okubo


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Operation of centrifugation

Operation of PCR amplification machine

Confirmation of nucleotide sequence by DNA sequencing

2. An E-mail News 34-72 version is sent to users.

3. Number of users registered to RIKEN DNA Bank is

6,651.

4. DNA materials (241,479) have been distributed to users

worldwide.

5. The size of the DNA collection archives is now third in

the world.

6. The Cre-zoo project was completed in 2008.

II. Technological development We have performed the following research projects to develop

a new technology for DNA Banking.

1. Detection of mutation of DNA samples

We have developed novel techniques for detecting the

mutation of genetic resources with high sensitivities and

reproducibilities. These techniques are used for validating the

quality of genetic resources.

2. Development of controlled expression system for genes

with modification enzymes

Recent progress in recombinant DNA technology focused on

the modification system for genes such as epigenesis, protein

degradation, phosphorylation and the addition of sugar and

lipid moieties to core proteins and DNAs. We have developed

a new two-vector or one-vector system for modifying gene

products with genes encoding methylation/demethylation-,

kinase/phosphatase-, acetylase/deacetylase-, ubiquitination/

deubiquitination-, sumoylation/desumoylation- and sugar/

lipid-related enzymes.

3. Development of a system for targeted gene delivery

using a specific promoter and generation of transgenic

mice with controlled gene expression

We have developed a regulated gene expression system using

reporter constructs of tissue-specific promoters and generated

novel transgenic mice with a Cre-loxP cassette with the tissue-

specific promoter (in collaboration with Tsukuba University

and the Experimental Animal Division of BRC). We are now

focusing on the promoters of the p53-targeted gene family.

4. Application of adenovirus vectors to cancer gene

therapy, regeneration biology and molecular biology

We have developed a novel gene delivery system for

cancerous cells and embryonic stem cells as well as for model

animals using tumor suppressor genes and suicide genes. We

have also developed an efficient system for gene transfer using

novel adenoviral vectors with E1-Rb mutants, chimeric fibers

and modified fibers for gall bladder cancer, biliary tract cancer

and liver cancer (in collaboration with Tsukuba University and

Sapporo Medical University). We have focused on embryonic

stem cells for the gene delivery of modified adenoviral


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vectors.

5. Development of gene expression system and evaluation

system of expressed genes in eukaryotic cells and animal

models

We have developed a novel strategy for evaluating gene

expression in chromatin and for evaluating the system for

expressed gene products in cells. We focused on the AP-1

family of transcription factors and on chromatin-modified

factors such as those involved in histone acetylation and

methylation as well as in sumoylation in eukaryotic cells and

model animals.

6. Efficacy of artificial promoter vectors

A controlled reporter system with the DNA binding sites

(cis-elements) of transcription factors has been developed

and examined of their efficacies for various cells including

neoplastic, normal diploid, and embryonic stem cells as well

as germ cells.

III. Evaluation of activities of the “National BioResource Project (NBRP; phase I)”.

The NBRP committee evaluated the banking activities of

RIKEN BRC with the highest score “S” among those of other

groups.

Administration care of MTA for Banking

Development of technology using Promoter-GFP DNA (Promoter Bank)

Initiation of adipocyte differentiation of MEF-JDP2(-/-) (DNA-set Bank)

IV. Introduction and distribution of our banking activities

We have set up the homepage http://www.brc.riken.jp/dna/en/

index.html and connected with the database of the National

Institute of Genetics. We have made e-mail news, catalogs and

other related notices of our DNA Bank in RIKEN BRC for

researchers worldwide. We have also introduced our banking

activities in annual domestic conferences such as those of the

Molecular Biology, Biochemistry, Cancer, Gene Therapy, as

well as in some international conferences such as the Cold

Spring Harbor meetings.


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Saito S., Sawai K., Minamihashi A., Ugai H., Murata

T., Yokoyama K.: “Derivation, maintenance and

differentiation of equine ES cells.” Nonhuman embryonic

steme cell. Protocols; vol. 1 (ed. by K. Turksen) (Humana

Press Inc., Totowa, NJ), Methods in Molecular Biology

329, pp.59-79 (2005).*

Seo E., Abei M., Wakayama M., Fukuda K., Ugai

H., Murata T., Todoroki T., Tanaka N., Hamada H.,

Yokoyama K.: “Effective gene therapy for biliary

tract cancer by a conditionally replicative adenovirus

expressing uracil phosphoribosyltransferase: Significance

of timing of 5-fluorouracil administration.” Cancer

Research 65, 546-552 (2005).*

Yokoyama K., Murata T., Ugai H., Suzuki E., Terashima

M., Kujime Y., Inamoto S., Hirose M., Inabe K.,

Yamasaki T.: “Recombinant Virus BANK for gene

delivery.” Science 307, 1722 (2005).*

Ugai H., Yamasaki T., Hirose M., Inabe K., Kujime Y.,

Terashima M., Liu B., Tang H., Zhao M., Murata T.,

Kimura M., Pan J., Obata Y., Hamada H., Yokoyama

K.: “Purification of infectious adenovirus in two hours

by ultracentrifugation and tangential flow filtration.”

Biochem. Biophys. Res. Commun. 331, 1053-1060

(2005).*

Ugai H., Murata T., Nagamura Y., Ugawa Y., Suzuki E.,

Nakata H., Kujime Y., Inamoto S., Hirose M., Inabe K.,

Terashima M., Yamasaki T., Liu B., Nakade K., Pan J.,

Kimura M., Saito I., Hamada H., Obata Y., Yokoyama

K.: “A database of recombinant viruses and recombinant

viral vectors available from the RIKEN DNA bank.” J.

Gene Med. 7, 1148-1157 (2005).*

Dietze E. C., Bowie M. L., Mrozek K., Caldwell E., Neal

C., Marjoram R. J., Troch M. M., Bean G. R., Yokoyama

K., Ibarra C. A., Seewaldt V. L.: “CREB-binding protein

regulates apoptosis and growth of HMECs grown in

reconstituted ECM via laminin-5.” J. Cell Science 118,

5005-5022 (2005).*

Publications

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【Original Papers】 (*Peer reviewed journals)

Kojima Y., Honda K., Kotegawa H., Kushihata F.,

Kobayashi N., Yokoyama K., Hamada H.: “Adenovirus-

mediated p53 gene transfer to the bile duct by direct

administration into the bile in a rat cholangitis model.” J.

Surgical Res. 128, 126-131 (2005).*

Ugai H., Inabe K., Yamasaki T., Murata T., Obata Y.,

Hamada H., Yokoyama K.: “Accumulation of infections

mutants in stocks during the propagation of fiver-

modified recombinant adenoviruses.” Biochem. Biophys.

Res. Commu. 337, 806-814 (2005).*

Yamasaki T., Noguchi M., Liu B., Nakade K., Wang P-C.,

Murata T., Yokoyama K.: “Jun dimenigation protein 2 : A

multifunctional transcription factor in mammalian cells.”

Current Genomics 6, 351-364 (2005).*

Mitsuzawa H., Kimura M., Kanda E., Ishihama A.:

“Glyceraldehyde-3-phosphate dehydrogenase and actin

associate with RNA polymerase II and interact with its

Rpb7 subunit.” FEBS Letters 579, 48- 52 (2005).*

Hayashi K., Watanabe T., Tanaka A., Furumoto T., Sato-

Tsuchiya C., Kimura M., Yokoi M., Ishihama A., Hanaoka

F., Ohkuma Y.: “Studies of Schizosaccharomyces pombe

TFIIE indicate conformational and functional changes in

RNA polymerase II at transcription initiation.” Genes to

Cells 10, 207-224 (2005).*

Abiko M., Akibayashi K., Sakata T., Kimura M.,

Kihara M., Itoh K., Asamizu E., Sato S., Takahashi H.,

Higashitani A.: “High-temperature induction of male

sterility during barley (Hordeum vulgare L.) anther

development is mediated by transcriptional inhibition.”

Sex Plant Reprod 18, 91-100 (2005).*

Wang Y., Onishi Y., Kakinuma N., Roy BC., Aoyagi T.,

Kiyama R.: “Alternative splicing of the human Kank

gene produces two types of Kank protein.” Biochem.

Biophys. Res. Commun. 330, 1247-1253 (2005).*

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Saito S., Yokoyama K., Tamagawa T., Ishiwata I.:

“Derivation and induction of the differentiation of animal

ES cells as well as human pluripotent stem cells derived

from fetal membrane.” Human Cell 18, 135-141 (2005).*

Liu B., Yamasaki T., Noguchi M., Murata T., Wang P.,

Yokoyama K.: “Gene silencing and histone modification

by JDP2, a AP-1 repressor during the differentiation of

embryonic stem cells.” Current Topics in Genetics 1,

1-17 (2006).*

Da L., Yokoyama K., Li T., Zhao M.: “Dual promoters

control the cell-specific expression of the human cell

death-inducing DFF45-like effector B gene.” Biochem. J.

393, 779-788 (2006).*

Jin C., Kato K., Chimura T., Yamasaki T., Nakade K.,

Murata T., Li H., Pan J., Zhao M., Sun K., Chiu R., Ito

T., Nagata K., Horikoshi M., Yokoyama K.: “Regulation

of histone acetylation and nucleosome assembly

by transcription factor JDP2.” Nature Structural &

Molecular Biology 13, 331-338 (2006).*

Hayashi R., Goto Y., Ikeda R., Yokoyama K., Yoshida

K.: “CDCA4 is an E2F transcription factor family-

induced nuclear factor that regulates E2F-dependent

transcriptional activation and cell proliferation.” J. Biol.

Chem. 281, 35633-35648 (2006).*

Lu Y-C., Song J., Cho H-Y., Fan G., Yokoyama K., Chiu

R.: “Cyclophilin a protects peg3 from hypermethylation

and inactive histone modification.” J. Biol. Chem. 281,

39081-39087 (2006).*

Wakayama M., Abei M., Kawashima R., Seo E., Fukuda

K., Ugai H., Murata T., Tanaka N., Hyodo I., Hamada H.,

Yokoyama K.: “E1A, E1B double-restricted adenovirus

with RGD-Fiber modification exhibits enhanced

oncolysis for CAR-deficient biliary cancers.” Clinical

Cancer Research. 13, 3043-3050 (2007).*

Nakade K., Pan J., Yoshiki A., Ugai H., Kimura

M., Liu B., Li H., Obata Y., Iwama M., Itohara S.,

Murata T., Yokoyama K.: “JDP2 suppresses adipocyte

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differentiation by regulating histone acetylation.” Cell

Death and Differentiaton 14, 1398-1405 (2007).*

Noguchi M., Yamasaki T., Masuzaki S., Higa A., Nakade

K., Pan J., Murata T., Nagata K., Shiota K., Yokoyama

K.: “Transcription factor Jun dimerization protein 2

(JDP2) has histone-chaperone activity that suppresses

gene expression.” Current Topics in Biochemical

Research 9, 27-44 (2007). *

Yamasaki T., Murata T., Jin C., Kato K., Noguchi M.,

Nakade K., Pan J., Nagata K., Yokoyama K.: “Assays

of nucleosome assembly and the inhibition of histone

acetyltransferase activity.” Nature Protocols DOI:

10.1038/nprot.2007.332 (2007).*

Saito S., Sawai K., Murayama Y., Fukuda K., Yokoyama

K.: “Nuclear Transfer to study the nuclear reprogramming

of human stem cells.” Methods in Molecular Biology

438, 151-169 (2008). *

Murata T., Shinozuka Y., Obata Y., Yokoyama K.:

“Phosphorylation of two eukaryotic transcription factors,

Jun dimerization protein 2 and activation transcription

factor 2, in Escherichia coli by Jun N-terminal kinase 1.”

Anal. Biochem. 376, 115-121 (2008).*

Hirano T., Ike F., Murata T., Obata Y., Utiyama H.,

Yokoyama K.: “Genes encoded within 8q24 on the

amplicon of a large extrachromosomal element are

selectively repressed during the terminal differentiation

of HL-60 cells.” Mutation Research 640, 97-106 (2008).*

Haase M. G., Klawitter A., Bierhaus A., Yokoyama

K., Kasper M., Geyer P., Baumann M., Baretton G.

B.: “Inactivation of AP1 Proteins by a Nuclear Serine

Protease Precedes the Onset of Radiation-Induced

Fibrosing Alveolitis.” Radiation Research 169, 531-542

(2008).*

Kovacevic-Grujicic N., Yokoyama K., Stevanovic M.:

“Trans-activation of the human sox3 promoter by mazin

NT2/D1 cells.” Arch. Biol. Sci., Belgrade 60, 379-387

(2008).*

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Kimura M.: “IRF2-binding protein-1 is a JDP2 ubiquitin

ligase and an inhibitor of ATF2-dependent transcription.”

FEBS Letters 582, 2833-2837(2008).*

Honda A., Hirose M., Inoue K., Ogonuki N., Miki H.,

Shimozawa N., Hatori M., Shimizu N., Murata T., Hirose

M., Katayama K., Wakisaka N., Miyoshi H., Yokoyama

K., Sankai T., Ogura A.: “Stable embryonic stem cell

lines in rabbits: potential small animal models for

human research.” Reproductive BioMedicine Online 17,

706-715 (2008).*

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Fukuda K., Abei M., Kawashima R., Ugai H., Seo E.,

Wakayama M., Murata T., Endo S., Hamada H., Hyodo I.,

Yokoyama K.: “E1A, E1B-double restricted replicative

adenovirus at low dose augments the efficacy of tumor-

specific suicide gene therapy for gallbladder cancer.”

Cancer Gene Therapy (in press).*

Sato I., Obata Y., Kasahara K., Nakayama Y., Fukumoto

Y., Yamasaki T., Yokoyama K., Saito T., Yamaguchi, N.:

“Differential trafficking of c-Src, Lyn, c-Yes, and Fyn

is specified by the state of palmitoylation in the SH4

domain.” J. Cell Science (in press).*

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Oral Presentations

Yokoyama K., Nakade K., Pan J., Liu B., Yamasaki T.,

Kimura M., Abei M., Murata T.: “Histone acetylation

and de-acetylation is critical for cell-differentiation in

response to retinoic acid (RA).” International Symposium

on Molecular cell Biology of Macrophages 2005, Omiya,

Saitama, Japan, pp.112, Jun. (2005).

Nakade K., Pan J., Liu B., Yamasaki T., Kimura

M., Murata T., Yokoyama K.: “Inhibition of histon

acetylation by transcription factor JDP2 is critical during

differentiation of F9 cells.” 70th Symposium, Molecular

Approaches to Controlling Cancer, Cold Spring Harbor,

NY. pp.166, Jun. (2005).

Yokoyama K., Yamasaki T., Liu B., Nakade K., Pan J.,

Kimura M., Murata T.: “Inhibition of histon acetylation

by transcription factor JDP2 is critical for differentiation

of F9 cells.” Mechanisms of Eukaryotic Transcription,

Cold Spring Harbor Meeting, Cold Spring Harbor, NY,

pp.321, Aug. (2005).

Murata T., Ugai H., Inabe K., Pan J., Hamada H.,

Yokoyama K.: “Genotypic study of accumulated

infectious mutants in stocks during the preparation

of fiber-modified recombinant adenoviruses.” Target

Definition & Vector Design, Cold Spring Harbor

Meeting, Cold Spring Harbor, NY, pp. 31 Nov. (2005).

1.

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3.

4.

Liu B., Ugai H., Pan J., Murata T., Hamada H.,

Yokoyama K.: “Purification of infectious adenovirus

in two hours by ultracentrifugation and tangential flow

filtration.” Target Definition & Vector Design, Cold

Spring Harbor Meeting, Cold Spring Harbor, NY, pp. 27

Nov. (2005).

Yokoyama K., J in C., Yamasaki T. , Murata T.:

“Regulation of histone acetylation and nucleosome

assembly by transcription factor JDP2.” International

Symposium on Molecular Cell Biology of Macrophages

and Dendritic cells 2006, Hongo, Tokyo, Japan, pp.102,

Jun. (2006).

Yokoyama K., Yamasaki T., Noguchi M., Ide Y., Liu B.,

Wang Y., Nakade K., Pan J., Kimura M., Jin C., Murata

T.: “Inhibition of histone acetylation by transcription

factor JDP2 is critical for differentiation of F9 cells.”

20th IUBMB International Congress of Biochemistry and

Molecular Biology and 11th FAOBMB Congress, Kyoto,

Japan, pp.770, Jun. (2006).

Murata T., Terashima M., Pan J., Kimura M., Yokoyama

K.: “Establishment of the Recombinant Virus Database

and the gene delivery system LICCA.” 20th IUBMB

International Congress of Biochemistry and Molecular

Biology and 11th FAOBMB Congress, Kyoto, Japan,

pp.323, Jun. (2006).

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【International Conferences】


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Kimura M., Ishihama A., Yokoyama K.: “Structure-

function analysis of the Rpb4 subunit of RNA

polymerase II in Schizosaccharomyces pombe.” 20th

IUBMB International Congress of Biochemistry and


Japan, pp.236, Jun. (2006).

Pan J., Murata T., Nakade K., Liu B., Yoshiki A., Itohara

S., Yokoyama K.: “AP-1 repressor JDP2 control cell

proliferation by regulating the cell cycle.” 20th IUBMB



pp.715, Jun. (2006).

Nakade K., Pan J., Yoshiki A., Ugai H., Murata T.,

Yokoyama K.: “JDP2 controls adipogenesis by regulating

the expression of C/EBPdelta via histone acetylation.”

20th IUBMB International Congress of Biochemistry and


Japan, pp.246, Jun. (2006).

Wang Y., Murata T., Pan J., Yokoyama K.: “Subcellular

localization of mouse JDP2 protein.” 20th IUBMB



pp.623, Jun. (2006).

Liu B., Inabe K., Noguchi M., Yamasaki T., Shiota K.,

Murata T., Yokoyama K.: “Gene therapy of neoplastic

cancer cells by a conditionally replicative adenovirus

expressing transcription factor JDP2.” 20th IUBMB


Biology and 11th FAOBMB Congress, Kyoto, Japan, pp.

246, Jun. (2006).

Yamasaki T., Noguchi M., Ide Y., Liu B., Yamaguchi N.,

Shiota K., Murata T., Yokoyama K.: “Analysis of DNA-

Binding domain, Histone-Binding domain and domain of

inhibition of histone acetyltransferase (INHAT) of JDP2

for transcriptional regulation.” 20th IUBMB International

Congress of Biochemistry and Molecular Biology and

11th FAOBMB Congress, Kyoto, Japan, pp.246, Jun.

(2006).

Wakayama M., Kawashima R., Abei M., Murata T.,

Hamada H., Yokoyama K.: “Enhanced Oncolysis and

Safety of a Conditionally Replicative Adenovirus (CRAd)

with RGD Fiber Modification for Biliary Cancers.” 20th

IUBMB International Congress of Biochemistry and


Japan, pp.593, Jun. (2006).

Noguchi M., Yamasaki T., Murata T., Liu B., Pan J.,

Kimura M., Shiota K., Yokoyama K.: “Analysis of

the mechanism of histone acetylation regulated by

transcription factor JDP2.” 20th IUBMB International

Congress of Biochemistry and Molecular Biology and

11th FAOBMB Congress, Kyoto, Japan, pp.245, Jun.

(2006).

Yokoyama K., Jin C., Yamasaki T., Noguchi M., Murata

T.: “Regulation of histone acetylation and nuclosome

assembly by transcription factor JDP2.” Mechanisms &

Models of Cancer, Cold Spring Harbor Meeting, Cold

Spring Harbor, NY, pp.305, Aug. (2006).

Pan J., Murata T., Kimura M., Nakade K., Inabe K.,

Yamasaki T., Kujime Y., Hirose M., Yamamoto M.,

Yamaoka S., Yokoyama K.: “RIKEN DNA Bank,

A Global Recombinant DNA Depository Institution

For Advancing Post-genomic Research.” 4th ISGO

International Conference on Structural Genomics,

Beijing, China, pp.188, Oct. (2006).

Pan J., Nakade K., Yamasaki T., Murata T., Yokoyama

K.: “AP-1 repressor JDP2 suppresses adipocyte

differentiation by regulating histone acetylation.” 16th

International Symposium on Molecular Cell Biology of

Macrophages 2007, Shizuoka, Japan, pp.75, Jun. (2007).

Pan J., Nakade K., Yamasaki T., Kimura M., Murata

T., Yokoyama K.: “AP-1 repressor JDP2 suppresses

adipocyte differentiation by regulating histone

acetylation.” Mechanisms of Eukaryotic Transcription,

Cold Spring Harbor Meeting, Cold Spring Harbor, NY,

pp.179, Aug. (2007).

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15.

16.

17.

18.

19.

20.


― 74 ―

Yokoyama K., Nakade K., Pan J., Murata T.: “AP-1

repressor JDP2 is histone chaperone to repress the viral

promoter activity.” 7th Awaji International Forum on

Infection and Immunity, Awaji, Japan, pp.74, Sep. (2007).

Nakade K., Pan J., Yamasaki T., Murata T., Yokoyama

K.: “AP-1 repressor JDP2 regulates differentiation and

proliferation of adipocytes and replicative senescence of

mouse embryonic fibroblasts.” Mechanisms & Models

of Cancer, Cold Spring Harbor Meeting, Cold Spring

Harbor, NY, pp.177, Aug. (2008).

Yokoyama K., Pan J. Nakade K., Murata T.: “Anti-viral

factor JDP2 regulates differentiation and proliferation

of adipocytes and replicative senescence.” 8th Awaji

International Forum on Infection and Immunity, Awaji,

Japan, pp.68, Sep. (2008).

21.

22.

23.

【Domestic Conferences】　Total 60

Documents

Gene Engineering Division - Riken