Experimental Animal Division · Experimental Animal Division RIKEN BRC Annual Report 2005 ～2007 ― 44 ― technicians. Each course includes theory and practice of the mouse facility

― 40 ―

Head

Experimental Animal Division

Goal

Programs

The Experimental Animal Division has two missions: (a) to

collect, preserve, conduct quality control of and distribute

high-quality mouse resources, and (b) to develop novel mouse

models and useful technologies for bioresource activities. Our

ultimate goal is to contribute to human welfare by facilitating

research in life sciences with our mouse resources. The mouse

is one of the most superior model organisms used in life

sciences to understand human health and overcome diseases.

Since 2002, RIKEN BRC has been designated as the central

core facility for mouse resources in Japan by the National

BioResource Project (NBRP) of MEXT. With support from

the scientific community, RIKEN BRC has collected over

3,200 mouse strains including genetically modified models,

ENU mutants, and inbred and wild-derived strains. RIKEN

BRC is a founding member of the Federation of International

Mouse Resources (FIMRe) together with The Jackson

Bioresource program: Collection, preservation, quality-

control and distribution of the mouse resources

MembersSenior Scientist, Head of Experimental Animal Division

Atsushi YOSHIKI, Ph.D. (2004.12~)

Senior Research Scientist

Fumio IKE, Ph.D. (2001.4~)

Senior Technical Scientist

Noriko HIRAIWA (2001.4~) Hatsumi NAKATA, Ph.D. (2007.4~)

Research Scientist

Kazuyuki MEKADA, Ph.D. (2003.8~) Yasuyuki KITAURA, Ph.D. (2006.3~）

Technical Staff Ⅱ

Ayumi MURAKAMI (2001.4~) Maiko IJUIN (2004.10~)

Masayo KADOTA (2006.4~) Mika OKAWA (2006.4~2008.1)

Assistant

Norie TSUDA (2001.12~2007.3)

Atsushi YOSHIKI, Ph.D.

Laboratory, European Mouse Mutant Archive (EMMA)

and other centers, and has participated in the International

Mouse Strain Resource (IMSR) to distribute mouse strains

worldwide. To meet research and social needs, our mice are

cleaned up to a specific pathogen-free state, strictly monitored

of their health, and accurately tested on their genetic

modifications and backgrounds. Phenotypic information of

the strains is frequently updated to enrich their value. With

these activities, RIKEN BRC plans to build mouse resources

that meet the highest global standards by 2010. The Material

Transfer Agreement (MTA) is used to protect the intellectual

property rights of the developer of the strains. Training

courses concerning advanced technologies are provided for

users to best use our resources. RIKEN BRC collaborates

with Asian and other overseas institutes to contribute to the

advancement of life sciences in the world.

I. Development program: Development of novel mouse

models and technologies necessary for program I.

II.

Experimental Animal DivisionRIKEN BRC Annual Report 2005 ～ 2007

― 41 ―

Student Trainee

Jan, MEI-LING (2007.4~)

Visiting Scientist

Chikako YOSHIDA-NORO, Ph.D. (2005.4~) Ikuko SAKAI (2005.8~)

Chino SASAOKA (2005.8~) Ryoji HYODO (2006.11~)

Nobuhiko ONDA (2005.8~2007.3) Kaori HIGUCHI (2006.11~2008.3)

Kayuri MURAKI (2006.11~2008.3)

Visiting Technician

Ayako KAJITA (2006.5~)

Agency Staff

Fujimi ARAI (2001.4~) Megumi KOBAYASHI (2001.4~)

Reiko KOJIMA (2001.4~) Akemi KOSHIYAMA (2001.4~)

Kyoko MEKADA (2001.4~) Yukiko SEKI (2001.4~)

Aya UEKI (2001.4~) Masaru ONUMA (2001.5~)

Atsushi CHOEI (2001.6~) Rika TAKASHIMA (2001.6~)

Teruo SAITO (2004.12~) Miki NAKAYAMA (2005.5~)

Chihiro YOKOYAMA (2005.10~) Kinuko ITO (2005.10~)

Yusuke OBA (2005.12~) Reiko KAWAI (2006.1~)

Hitomi TAKAHASHI (2006.4~) Masashi YOKOTA (2006.4~)

Chiharu OKUBO (2006.5~) Akemi YASUI (2006.5~)

Masashi OZAWA (2006.6~) Masako KITO (2006.9~)

Hiroyuki OKAMOTO (2006.9~) Tomomi HASHIMOTO (2007.4~)

Makiko IIZUMI (2007.4~) Naoki OTAKA (2007.4~)

Chieko YAMADA (2007.4~) Tomoe SAKAI (2007.4~)

Maki YAMAMOTO (2007.6~) Miki NAKAYAMA (2007.11~)

Chie FUJIMOTO (2008.4~) Aya MIMORI (2008.4~)

Kyuichi TAGUMA (2008.4~) Tomoko TAKAHASHI (2008.4~)

Naoki HIRANO (2008.6~) Hiromi HISAMATSU (2007.6~)

Fumie MIYAZAKI (2001.4~2008.3) Masami ICHIKAWA (2007.3~2008.3)

Hitomi OKANO (2007.3~2008.3) Sanae MIYAMOTO (2006.11~2007.3)

Masayuki SAITO (2006.10~2007.11) Satoe NAKAMURA (2005.4~2008.3)

Mika KOMURO (2005.6~2007.2) Yoko ONUKI (2005.10~2007.10)

Chie HOSHI (2005.11~2007.11) Naomi ISO (2006.2~2007.3)

Junichi MASUDA (2006.3~2008.7) Emiko FUKUDA (2006.4~2008.3)

Yasuko NISHIKAWA (2006.4~2007.9) Ayako KAJITA (2006.5~2007.3)

Katsumi WATANABE (2007.11~2008.5) Yuko FUJIMOTO (2001.4~2007.3)

Contract Staff

Noriko KATAOKA (2001.4~) Tomohiro OKUBO (2002.2~)

Keiko TOMIYAMA (2002.3~) Noriko NOGUCHI (2004.1~)

Kunihiro SAKURAI (2005.2~) Mariko HASEGAWA (2006.3~)

Takeshi MATSUZAKI (2006.3~) Katsuhiko YAGISHITA (2006.7~)

Takashi FURUYA (2007.2~) Takayoshi KOSHIDO (2007.4~)

Takeshi NAGAO (2007.4~) Masaki YAMAGUCHI (2007.4~)

Kiyohiko ASANO (2007.8~) Masataka ASANO (2007.12~)


― 42 ―

Programs

1. Bioresource Program

(1) Collection, preservation and distribution of mouse

strains

This program has been operated in cooperation with research

scientists, technical scientists, technical staffs, agency and

contract staffs. In regard to the annual plan for the operation

of the Experimental Animal Division, we have obtained

useful advice and suggestions from distinguished members

of the BRC Experimental Animal Steering Committee, BRC

Advisory Council, Promotion Advisor and RIKEN Advisory

Council. MTAs for the deposition and distribution of

mouse resources have been used to protect the intellectual

property right of the Developer, and to clarify the terms and

conditions of use for the Recipient of the biological resources,

respectively. We have collected over 3,200 strains mainly

developed in Japan, such as inbred, transgenic, knockout, Cre-

driver, ENU mutant and wild-derived strains, as well as gene-

trap ES clones. These strains are useful models for the study

of cancer, immunity and allergy, endocrine diseases, brain

and neurological disorders, development and differentiation

abnormalities, and sensory organ abnormalities (Fig.1).

Okamoto, Hirano, Koshido, Ike, Yoshiki, Asano(T), Matsuzaki, KitauraAsano(K), Sakurai, Yamaguchi, Saito, Okubo(T), Yokota, Masuda, Taguma, Furuya

Mitsunari, Hasegawa, Kojima, Arai, Oba, Nagao, Onuma, Otaka, Choei, Takahashi(H)Yamamoto, Hashimoto, Iizumi, Kawai, Nakayama, Hisamatsu, Kajita, Okubo(C), Nakayama, Fujisawa

Kobayashi, Sato, Mimori, Sakai, Takahashi(T), Ito, Koshiyama, Ozawa, TakanoOkada, Murakami, Shima, Fujibayashi, Yokoyama, Hiraiwa, Kataoka, Kito, Tsukahara

Fujimoto, Tomiyama, Takashima, Yamada, Yasui, Ijuin, Kadota, Ueki

Takehiko FUJISAWA (2008.4~) Haruo SUZUKI (2004.4~2007.3)

Sei CHOEI (2001.4~2007.2) Katsuya ONUKI (2001.4~2008.2)

Chieko YAMADA (2001.4~2008.3) Hiroyuki NUMAJIRI (2001.8~2007.5)

Naoki OTAKA (2001.9~2008.3) Masaki MIYAKE (2006.8~2007.3)

Asami SAGARA (2006.2~2007.12) Yasuhiro YOKOTA (2002.10~2007.1)

Minehisa SUZUKI (2007. 4~2007. 8)


― 43 ―

Mouse strains with high-demand from the community are

maintained as live animals, and others with low-demand

are preserved as frozen embryos. With the release of new

mouse resources and changes in circumstances, we revised

the distribution fees in March, 2008. The number of

registered users is 2,459 (523 for overseas) as of July, 2008.

We have distributed 8,453 (1,475 for overseas) mice to 436

(222 overseas) organizations since 2002. Recipients of our

mice included both academic (88%) and for-profit (12%)

organizations.

(2) Cleanup of mouse strains

The cleanup of mouse strains deposited to RIKEN BRC has

contributed to the high quality standard of animal experiments

in Japan. All the strains are serologically tested for infection

by eight dangerous pathogens. Based on the result of

serological tests, mice are transferred to the Bio-bubble

housing facilities either in negative or positive pressure. Mice

are bred to establish colonies for rederivation by in vitro

fertilization and embryo transfer or Cesarean section. Jcl:ICR

and BALB/cA-nu/+ females are used as recipients of embryo

transfer and as foster nursing mothers, respectively. BALB/

cA-nu/+ mothers have excellent nursing capability to accept

various strains in BRC.

(3) Cryopreservation of mouse embryos and sperm

The cryopreservation of embryos and sperm is a key

technology for the successful operation of the mouse

resource center. Two-cell stage embryos are stored frozen

in a vitrification solution containing ethylene glycol, Ficoll

and sucrose (EFS). The sperm from mutant and genetically

modified strains is also cryopreserved using raffinose and

skim milk as cryoprotective agents. The recovery rate of

each frozen strain is carefully assessed prior to closing live

colonies. The distribution of frozen strains is conducted after

assessing their recovery. By July 2008, 2,123 strains have

been preserved as frozen embryos or sperm.

(4) Quality control

(i) Microbiological monitoring

After cleanup treatments, the mice are monitored by a 2nd

round of microbiological tests for 19 pathogens. If the mice

are free of specific pathogens, they are transferred to the

BRC breeding rooms in the barrier. Periodic microbiological

monitoring of 19 or 22 major pathogens has been done in

every rack of the facility using sentinel mice (70,770 tests

for 3,681 mice from October 2005 to July 2008). The

environment of the facility is quarterly monitored for bacteria

and fungi at 512 points.

(ii) Genetic monitoring

Genetically modified strains such as transgenic and knockout

mice are genotyped using PCR protocols with allele-

specific primers. The genotyping PCR protocols for over

400 strains with high-demand is available on our website as

downloadable pdf files. Genetic backgrounds of congenic

strains are monitored with 7 sets of simple sequence length

polymorphism (SSLP) markers at 75-88 loci in each set.

Inbred and wild-derived strains are also monitored with 15

standard biochemical genetic markers.

(iii) Immunological profiling

The immunological profiling of 43 Inbred and wild-derived

strains is conducted using flow cytometry by the fluorescent

labeling of spleen cell differentiation antigens.

(5) Collection of relevant information and advertisement

In collaboration with the BRC Information Division, our

website is regularly updated and enriched of its content.

Publications by users and relevant literature about the

strains are frequently surveyed by direct email letters and

public databases. A revised list of our strains is submitted

monthly to the IMSR and distributed to the international

scientific community. Periodic e-mail newsletters have been

distributed to over 2,000 users. The e-mail newsletters contain

an article entitled “Mouse of the Month” (Fig.2), topic news

and a report of our recent activities. The mouse resources

and activities of the division are advertised in symposia and

academic meetings.

(6) Training course

The Experimental Animal Division provides training

courses for animal facility managers and laboratory animal

Figure 1. Framework of collection and distribution of mouse resources for advancement of life sciences.


― 44 ―

technicians. Each course includes theory and practice of the

mouse facility management and quality control programs such

as microbiological and genetic monitoring tests. We have thus

far accepted 12 trainees from local pharmaceutical and other

companies, the National Laboratory Animal Center of Taiwan,

and the Lanzoh Institute of Biological Products of China.

(7) International collaboration

RIKEN BRC is a founding member of the Federation

of International Mouse Resources (FIMRe). FIMRe is

a collaborating group of mouse repository and resource

centers worldwide, including The Jackson Laboratory and

the European Mouse Mutant Archive (EMMA). We have

participated in IMSR, a one-stop shop of mouse strains

available worldwide. RIKEN BRC promotes collaboration

with Asian countries. We are a founding member of the Asian

Mouse Mutagenesis and Resource Association (AMMRA).

On November 26, 2007, the Bio-Evaluation Center of Korea

Research Institute of Bioscience and Biotechnology and

RlKEN BRC entered into a Memorandum of Understanding to

promote cooperation in areas of mutual interest in laboratory

animal sciences. We started mutual visits with the Laboratory

Animal Center, Institute of Cytology and Genetics, Siberia

Branch of Russian Academy of Sciences in 2008.

(8) Others

Our Division has been designated as a NBRP-rat sub-center

in collaboration with Prof. Serikawa, Kyoto University. Our

mission is to establish a backup storage of NBRP rat strains as

frozen embryos and sperm.

2. Development ProgramOur division develops novel mouse resources and relevant

technologies necessary for the collection, preservation,

quality-control and distribution of the mouse resources. The

following development programs were carried out in the fiscal

years 2006-2008.

(1) Development of novel mouse resources

(i) RIKEN BRC grant for R&D of genetically modified

mouse strains

RIKEN BRC publicly called for applicants in the fiscal years

2007 and 2008 for research and development programs

to generate novel genetically modified mouse strains

of immediate demand by the research community. The

applicants were asked to propose a design of gene constructs

for genetic modifications, and to produce and submit the

recombinant gene constructs to BRC. BRC separately orders

the third parties to make mouse strains after an open bid. The

specialist committee members including those of the BRC

Experimental Animal Steering Committee have selected 12

academic organizations to generate 103 gene constructs for

genetic modifications in 2007, and 11 organizations for 291

genes in 2008.

(ii) Development of genetically uniform mouse strains

Transgenic and knockout mouse strains with high-demand

such as GFP and human disease models were backcrossed

with standard inbred strains to produce genetically uniform

congenic strains.

(iii) Development of Cre-Zoo

Conditional knockout mouse resources have been generated

worldwide for the functional analysis of genes. Tissue-

specific Cre-transgenic mouse strains are essential tools

for dissecting spatiotemporal gene functions together with

conditional knockout mice. In cooperation with the Gene

Engineering Division, we developed several lines of Cre-

transgenic mouse strains driven by tissue-specific promoters.

Figure 2. An excellent mouse model is introduced as “Mouse of the Month” in our e-mail mews.


― 45 ―

(2) Development of technologies for quality control and

strain characteristic database

(i) Development of a fine detection method for pathogens

A highly sensitive multiplexed microfluidic immunoassay

system for rapidly detecting pathogenic microbes with a

microvolume of blood sample was developed. Pathogenic

antigens were extracted and purified for the detection system

and positive controls. Genomic materials of 21 pathogenic

microbes were also been prepared as positive controls for

microbiological tests.

(ii) Microsatellite and SNP data

We prepared 7 sets of SSLP markers at 75-88 loci in each set

to examine genetic backgrounds. SNP analysis was carried

out in 153 strains including inbred, congenic, recombinant

inbred and wild-derived strains to clarify their genetic quality

using 15K SNPs of B6-MSM and the Illumina Golden Gate

Mouse SNP panel. These SNP data clearly demonstrated

the genetic relationship of the strains and the subtle genetic

differences among C57BL/6 substrains.

(iii) Development of genotyping methods for genetically

modified strains

We d e v e l o p e d a “ K O s u r v e y ” P C R p r o t o c o l f o r

simultaneously detecting different regions of complex

transgenes, and used it to screen mice in the quarantine and

barrier facilities. Transgenic strains were analyzed with their

genomic sequences flanking transgenes. The information

obtained was used to determine the integration site of

transgenes and to set up genotyping PCR protocols for hemi-

or homo-zygous distinction. Allele-specific PCR protocols

for accurately detecting point mutations and SNPs of the

strains were developed. These technologies and information

have contributed to obtaining accurate information on genetic

modifications and genetic backgrounds.

(iv) RIKEN BRC Mouse Phenome Database (RMPD)

RMPD was developed in collaboration with the BRC

Information Division and made available in our website in

March, 2008. The database includes external measures, blood

pressures, blood cell counts, and blood biochemistry measures

of 140 mouse strains. The data could be displayed as bar

graphs, two-dimensional plots and lists. We also collected

images of the coat colors, histopathological sections and

X-ray images of the skeleton of 104 various inbred and mutant

mouse strains. Behavioral phenotypes were also recorded

using a digital video camera.

(v) Development of novel phenotype data and disease

models

We started collaborations with the Technology and

Development Team for Mouse Phenotype Analysis, Japan

Mouse Clinic and other groups to enrich the phenotype data of

our mice and develop novel human disease models.

(3) Other projects

(i) NBRP genome information upgrading program

The end sequencing of 800,000 cDNA clones from wild-

derived strains, MSM, HMI, PGN2 and KJR was carried

out by collaborating with Prof. Shiroishi of the National

Institute of Genetics and Dr. Abe of the BRC Technology and

Development Team for Mammalian Cellular Dynamics.

(ii) NBRP fundamental technology upgrading program

(2007-2008)

A collaborative program with Prof. Serikawa (Kyoto

University), Prof. Nakagata (Kumamoto University) and

Dr. Ogura (Bioresource Engineering Division) entitled

“Development of transportation system for the mouse and

rat resources” was carried out to develop high-performance

shipping containers for live rodents and their embryos and

sperm.

(iii) Development of Cre-driver strains for neural circuit

genetics

In collaboration with Prof. Tonegawa (MIT), Dr. Itohara

(RIKEN BSI), Dr. Abe (BRC) and Dr. Obata (BRC), we

started a project to generate Cre-driver transgenic mouse

strains for the functional analysis of the neural circuit.


― 46 ―

Ugai H., Murata T., Nagamura Y., Ugawa Y., Suzuki

E., Nakata H., Kujime Y., Inamoto S., Hirose M.,

Inabe K., Terashima M., Yamasaki T., Liu B., Nakade

K., Pan J., Kimura M., Saito I., Hamada H., Obata Y.,

Yokoyama K.K.: “A database of recombinant viruses

and recombinant viral vectors available from the RIKEN

DNA bank.” J Gene Med 7, 1148-1157 (2005).*

Toyo-Oka K., Sasaki S., Yano Y., Mori D., Kobayashi

T., Toyoshima YY., Tokuoka SM., Ishii S., Shimizu

T., Muramatsu M., Hiraiwa N., Yoshiki A., Wynshaw-

Boris A., Hirotsune S.: “Recruitment of katanin p60 by

phosphorylated NDEL1, an LIS1 interacting protein,

is essential for mitotic cell division and neuronal

migration.” Hum Mol Genet 14, 3113-3128 (2005).*

Sasaki S., Mori D, Toyo-oka K., Chen A., Garrett-Beal

L., Muramatsu M., Miyagawa S., Hiraiwa N., Yoshiki

A., Wynshaw-Boris A., Hirotsune S.: “Complete loss of

Ndel1 results in neuronal migration defects and early

embryonic lethality.” Mol Cell Biol 25, 7812-7827

(2005).*

Nakamura K., Suzuki Y., Inoue N., Noro C., Suzuki A.:

“Structural characterization of neutral glycosphingolipids

by thin-layer chromatography coupled to matrix-assisted

laser desorption/ionization quadrupole ion trap time-of-

flight MS/MS.” Aral. Chem. 78, 5736-5743 (2006).*

Sato J.J., Tsuru Y., Hirai K., Yamaguchi Y., Mekada

K., ., Takahata N., Moriwaki K.: “Further evidence for

recombination between mouse hemoglobin beta b1 and

b2 genes based on the nucleotide sequences of intron,

UTR, and intergenic spacer regions.” Genes Genet Syst

81, 201-209 (2006).*

Yoshiki A., Moriwaki K.: “Mouse phenome research:

implications of genetic background.” Ilar J 47, 94-102

(2006).*

Yonezawa S., Yoshizaki N., Kageyama T., Takahashi

T., Sano M., Tokita Y., Masaki S., Inaguma Y., Hanai

Publications

1.

2.

3.

4.

5.

6.

7.

【Original Papers】 (*Peer reviewed journals)

A., Sakurai N., Yoshiki A., Kusakabe M., Moriyama A.,

Nakayama A.: “Fates of Cdh23/CDH23 with mutations

affecting the cytoplasmic region.” Hum Mutat 27, 88-97

(2006).*

Kaneko S., Aki I., Tsuda K., Mekada K., Moriwaki K.,

Takahata N., Satta Y.: “Origin and evolution of processed

pseudogenes that stabilize functional Makorin1 mRNAs

in mice, primates and other mammals.” Genetics 172,

2421-2429 (2006).*

Inoue K., Noda S., Ogonuki N., Miki H., Inoue S.,

Katayama K., Mekada K., Miyoshi H., Ogura A.:

“Differential developmental ability of embryos cloned

from tissue-specific stem cells.” Stem Cells 25, 1279-

1285 (2007).*

Ike F., Bourgade F., Ohsawa K., Sato H., Morikawa S.,

Saijo M., Kurane I., Takimoto K., Yamada Y K., Jaubert

J., Berard M., Nakata H., Hiraiwa N., Mekada K.,

Takakura A., Itoh T., Obata Y., Yoshiki A., Montagutelli

X.: “Lymphocytic choriomeningitis infection undetected

by dirty-bedding sentinel monitoring and revealed after

embryo transfer of an inbred strain derived from wild

mice.” Comparative Medicine 57, 272-281 (2007).*

Nakade K., Pan J., Yoshiki A., Ugai H., Kimura M.,

Liu B., Li H., Obata Y., Iwama M., Itohara S., Murata

T., Yokoyama KK.: “JDP2 suppresses adipocyte

differentiation by regulating histone acetylation.” Cell

Death Differ (2007).*

Shinmen A., Honda A., Ohkawa M., Hirose M.,

Ogonuki N., Yuzuriha M., Miki H., Mochida K., Inoue

K., Abe K., Ito M., Ogura A.: “Efficient production of

intersubspecific hybrid mice and embryonic stem cells

by intracytoplasmic sperm injection.” Mol Reprod Dev

(2007).*

Tanaka S., Miura I., Yoshiki A., Kato Y., Yokoyama H.,

Shinogi A., Masuya H., Wakana S., Tamura M., Shiroishi

T.: “Mutations in the helix termination motif of mouse

8.

9.

10.

11.

12.

13.


― 47 ―

type I IRS keratin genes impair the assembly of keratin

intermediate filament.” Genomics 90:703-711 (2007).*

Endoh K., Mochida K., Ogonuki N., Ohkawa M. “The

developmental ability of vitrified oocytes from different

mouse strains assessed by parthenogenetic activation

and intracytoplasmic sperm injection.” Journal of

Reproduction and Development 53,1199-1206 (2007).*

Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto

H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-

Boris A., Yoshiki A., Hirotsune S.: “Protein phosphatase 4

catalytic subunit regulates Cdk1 activity and microtubule

organization via NDEL1 dephosphorylation.” J Cell Biol

180: 1133-1147 (2008).*

14.

15.

Hirano T., Ike F., Murata T., Obata Y., Uchiyama H.,

Yokoyama K.: “Genes encoded within 8q24 on the

amplicon of a large extrachromosomal element are

selectively repressed during the terminal differentiation

of HL-60 cells.” Mutation Research 640, 97-106 (2008).*

Takimoto K. , Taharaguchi M., Ike F. , Yamada

Y.: “Detect ion of the ant ibody to lymphocytic

choriomeningitis virus in sera of laboratory and newly

isolated strains by ELISA using purified recombinant

nucleoprotein.” Experimental Animals 57, No.4, 357-365

(2008).*

Motokawa M., Harada M., Mekada K., Shrestha KC.:

“Karyotypes of Soriculus nigrescens and Episoriculus

caudatus from Nepal (Soricomorpha, Soricidae).”

Integrative Zoology (in press).*

16.

17.

18.

Oral Presentations

Yoshiki A., Mekada K., Nakata H., Hiraiwa N., Ike F.,

Mochida K., Moriwaki K., Obata Y.: “Establishment of

mouse strain resources in RIKEN BioResource Center.”

International Symposium of the Korean Association for

Laboratory Animal Science, Seoul, Korea, Jun. (2005).

Satoko K., Tsuda K., Mekada K., Moriwaki K., Takahata

N., Satta Y.: “Rapid turnover of regulatory processed

pseudogenes of Makorin1 in rodents and primates.”

Molecular Biology & Evolution 2005, Auckland, New

Zealand, Jun. (2005).

Yoshiki A.: “The RIKEN BioResource Center in the

International Network of Mouse Strain Resources.”

Korean Society for molecular and cellular biology

meeting, Seoul, Korea, Sep. (2005).

Fuchikami T., Mise N., Sugimoto M., Kobayakawa S.,

Kondo M., Ike F., Abe K.: “Dynamics of global gene

expression changes during mouse primordial germ cell

development.” International Symposium on Germ Cells,

Epigenetics, Reprogramming and Embryonic Stem Cells,

Kyoto, Japan, Nov. (2005).

1.

2.

3.

4.

【International Conferences】

Mise N., Fuchikami T., Sugimoto M., Kobayakawa

S., Yuzuriha M., Ike F., Tada T., Ogawa T., Kanaya S.,

Noce T., Abe K.: “Classification of embryo-derived stem

cells and germ cells by genome-wide gene expression

profiling.” International Symposium on Germ Cells,

Epigenetics, Reprogramming and Embryonic Stem Cells,

Kyoto, Japan, Nov. (2005).

Sugimoto M., Mekada K., Karashima Y., Yuzuriha M.,

Ko S. H. M., Nagaraja, Tan S. S., Takagi N., Abe K.:

“Narrowing down the position of the t-complex recessive

lethal mutation tclw5 into 180kb by BAC rescue.” 19th

International Mouse Genome Conference, Strasbourg,

France, Nov. (2005).

Mekada K., Arai F., Murakami A., Oota S., Moriwaki

K., Obata Y., Yoshiki A.: “Analysis of a new recessive

mutant with abnormal walking.” 19th International

Mouse Genome Conference, Strasbourg, France, Nov.

(2005).

Mise N., Fuchikami T., Sugimoto M., Kobayakawa

5.

6.

7.

8.


― 48 ―

9.

10.

11.

12.

13.

14.

15.

S., Yuzuriha M., Ike F., Tada T., Ogawa T., Kanaya S.,

Noce T., Abe K.: “Classification of embryo-derived stem

cells and germ cells by genome-wide gene expression

profiling.” 20th IUBMB International Congress of

Biochemistry and Molecular Biology and 11th FAOBMB

Congress, Kyoto, Japan, Jun. (2006).

Doi T., Mise S., Hiraiwa N., Ike F., Yoshiki A., Kasai

K., Obata Y.: “Transcription factor NF-kB is essential at

neonatal stage.” 20th IUBMB International Congress of

Biochemistry and Molecular Biology and 11th FAOBMB

Congress, Kyoto, Japan, Jun. (2006).

Ike F., Yoshiki A.: “Lymphocytic choriomeningitis virus

(LCMV), re-emerging pathogen.” 2nd AALAS Congress

in 2006, Jeju, South Korea, Aug. (2006).

Mise N., Yuzuriha M., Kondo M., Ike F., Araki K., Tada

T., Ogawa T., Kanaya S., Noce T., Abe K.: “Classification

and characterization of ES, EG and primordial germ

cells having different sex chromosome compositions by

microarray-based expression profiling.” 2nd International

Conference on X-Inactivation, Paris, France, Sep. (2006).

Moriwaki K., Ike F., Takakura A.: “Establishment

of detection system for LCMV infection in RIKEN

BioResource Center and CIEA.” US Japan meeting 2006,

Salt Lake City, USA, Oct. (2006).

Fuchikami T., Mise N., Sugimoto M., Kobayakawa

S., Kondo M., Ike F., Abe K.: “Dynamics of global

gene expression in primordial germ cells during mouse

development.” Cold Spring Harbor Laboratory 2006

Meeting on Germ Cells, Cold Spring Harbor, USA, Oct.

(2006).

Nakata H., Kitaura Y., Mekada K., Murakami A., Obata

Y., Yoshiki A.: “The RIKEN BioRessource Center to

disseminate the highest quality mouse resource for

biomedical research.” Super Computing 2006, Florida,

USA, Nov. (2006).

Inoue K, Ogonuki N., Miki H., Noda S., Inoue S.,

Katayama K., Mekada K., Miyoshi H., Ohura A.:

“Differential developmental ability of embryos cloned

from tissue-specific stem cells.” The 33rd Annual

Conference of International Embryos Transfer Society,

Kyoto, Japan, Jan. (2007).

Nakamura K., Suzuki Y., Goto-Inoue N., Yoshida-

Noro C., Suzuki A.: “TLC-MALDI-MS for Neutral

Glycosphingolipids”, Glycobiology and Sphingobiology

2007 (GS2007): Hakomori Commemorative Forum ,

Tokushima, Feb. (2007).

Ike F., Aoki H., Morikawa S., Yoshiki A., Yamagata Y.:

“Identification of mouse antibody reaction by multi-

channel microfluidic immunoassay chip using small

amounts of diluted serum.” FELASA-ICLAS Joint

Meeting 2007, Cernobbio, Italy, Jun. (2007).

Ike F., Kajita A., Aoki H., Kase H., Nagamune T.,

Morikawa S., Obata Y., Yamagata Y.: “Detection of

emerging zoonotic infection in mice by high sensitive

multiplexed microfluidic immunoassay system.” 11th Int.

Conf. on Miniaturized Systems for Chemistry and Life

Sciences (μTAS 2007), Paris, France, Oct. (2007).

Aoki H., Kajita A., Kaneko A., Ishihara M., Hara T.,

Nonaka H., Yamagata Y., Ike F., Kase H.: “Development

of new rapid multiplex microfluidic chip system for

research animal serology monitoring.” 58th AALAS

National Meeting, Charlotte, USA, Oct. (2007).

Ike F., Kajita A., Yoshiki A., Obata Y., Aoki H., Kase

H., Yamagata Y., Morikawa S.: “Serological profiling

of lymphocytic choriomeningitis infected wild-derived

mice.” 58th AALAS National Meeting, Charlotte , USA,

Oct. (2007).

Tanaka S., Miura I., Yoshiki A., Kato Y., Yokoyama H.,

Shinogi A., Masuya H., Wakana S., Tamura M., Shiroishi

T.: “Abnormal Assembly of Keratin Intermediate

Filaments in Mouse Mutations of Type I Inner Root

Sheath Keratin.” 21st International Mammalian Genome

Conference (IMGC2007), (IMGC), Kyoto, Oct.-Nov.

(2007).

16.

17.

18.

19.

20.

21.


― 49 ―

22.

23.

24.

25.

26.

27.

28.

Araki K., Takeda N., Yoshiki A., Yamada G., Nakagata N.,

Shiroishi T., Moriwaki K., Yamamura K.: “Establishment

of embryonic stem cell lines derived from Msm/ms

strain originated from mus musculus molossinus.”

21st International Mammalian Genome Conference

(IMGC2007), Kyoto, Oct.-Nov. (2007).

Oota S., Mekada K., Arai F., Obata Y., Fukami K.,

Yoshiki A.: “Four-Dimensional quantitative analysis

on the gait of mutant mice by using the motion capture

technology.” 21st International Mammalian Genome

Conference (IMGC2007), Kyoto, Oct.-Nov. (2007).

Sato J. J., Yamaguchi Y., Ueta J., Suzuki H., Chunyan W.,

Kryukov A. P., Mekada K., Takahata N., Moriwaki K.:

“Genetic characterization of the Wild-derived house mice

in east asia.” 21st International Mammalian Genome

Conference (IMGC2007), Kyoto , Oct.-Nov. (2007).

Miura I., Tanaka S., Yokoyama H., Shinogi A., Kobayashi

K., Kaneda H., Toyoda T., Yoshiki A., Yonekawa H.,

Kikkawa Y., Kunieda T., Tamura M., Masuya H., Wakana

S., Shiroishi T.: “Hair Morphological Mutants Generated

in the RIKEN Mutagenesis Project.” 21st International

Mammalian Genome Conference (IMGC2007), (IMGC),

Kyoto, Oct.-Nov. (2007).

Yokota H., Kakusho N., Mekada K., Murakami A.,

Nakamura S., Ito M., Kase K., Oota S., Fukami K.,

Masuya H., Wakana S., Moriwaki K., Shiroishi T.,

Himeno R., Obata Y., Yoshiki A.: “High-Resolution

full color digital mouse anatomy by three-dimensional

internal structure microscopy.” 21st International

Mammalian Genome Conference (IMGC2007), Kyoto,

Oct.-Nov. (2007).

Kitaura Y., Mekada K., Nakata H., Matsushima Y., Mei-

Ling J., Shiroishi T., Moriwaki K., Obata Y., Yoshiki

A.: “Immunological profiling of laboratory inbred

and wild-derived mouse strains developed in Japan.”

21st International Mammalian Genome Conference

(IMGC2007), Kyoto, Oct.-Nov. (2007).

Yoshiki A., Ike F., Hiraiwa N., Nakata H., Mekada K.,

Kitaura Y., Mochida K., Kadota M., Murakami A., Fujita

M., Ohkawa M., Ogura A., Abe K., Moriwaki K., Obata

Y.: “RIKEN BRC to establish mouse resources of the

highest global standards.” 21st International Mammalian

Genome Conference (IMGC2007), Kyoto, Oct.-Nov.

(2007).

Mekada K., Abe K., Murakami A., Nakamura S., Obata

Y., Yoshiki A.: “Which C57BL/6 substrain is used for

the background strain of your mouse?” 21st International

Mammalian Genome Conference (IMGC2007), Kyoto,

Oct.-Nov. (2007).



M., Okawa M., Ogura A., Abe K., Moriwaki K., Obata

Y.: “The highest quality mouse resources for global

biomedical researchers from Riken BRC.” Satellite

Meeting. The 10th Annual Meeting of Chinese-Taipei

Society of Laboratory Animal Science. Taipei, Dec.

(2007).



M., Ogura A., Abe K., Wakana S., Moriwaki K., Obata

Y.: “Distribution of the highest quality mouse resources

from Riken BioResource Center.” The 6th International

Conference on Bioinformatics of Genome Regulation

and Structure, Novosibirsk, Jun. (2008).

Mekada K., Murakami A., Abe K., Moriwaki K., Obata Y.,

Yoshiki A.: “Genetic variation of mouse inbred strain”,

The 6th International Conference on Bioinformatics of

Genome Regulation and Structure, Novosibirsk, Jun.

(2008).

29.

30.

31.

32.

【Domestic Conferences】　Total 68

Documents

Experimental Animal Division · Experimental Animal Division RIKEN BRC Annual Report 2005 ～2007 ― 44 ― technicians. Each course includes theory and practice of the mouse facility