GANPAT UNIVERSITY FACULTY OF SCIENCE TEACHING AND ... · A. Carbohydrates: Iodine test, Molisch’s test, enedict’s test, arfoed test, ial’s test and Saliwanoff’s test. B. Proteins:

GANPAT UNIVERSITY

FACULTY OF SCIENCE

TEACHING AND EXAMINATION SCHEME Programme Bachelor of Science Branch/Spec. Microbiology

Semester III

Effective from Academic Year 2015-16

Effective for the batch Admitted in June 2015

Subject Code

Subject Name

Teaching scheme Examination scheme (Marks)

Credit Hours (per week) Theory Practical

Lecture(DT) Practical(Lab.) Lecture(DT) Practical(Lab.) CE SEE Total CE SEE Total

L TU Total P TW Total L TU Total P TW Total UMBA301MBP MICROBIAL

PHYSIOLOGY 03 03 03 03 40 60 100

UMBA302SWM SOIL AND WATER MICROBIOLOGY 03 03 03 03 40 60 100

UPMA303PRA PRACTICAL MODULE-III 03 03 06 06 100 100

UCHA301IPC INORGANIC AND PHYSICAL CHEMISTRY-III

03 03 03 03 40 60 100

UCHA302OAC ORGANIC AND ANALYTICAL CHEMISTRY-IV

03 03 03 03 40 60 100

UPCA303PRA PRACTICAL MODULE-III 03 03 06 06 100 100

UENA301ENG ENGLISH-III 02 02 02 02 40 60 100

OPEN SUBJECT – 1 02 02 02 02 40 60 100

Total 16 16 06 06 16 16 12 12 240 360 600 200 200

GANPAT UNIVERSITY

FACULTY OF SCIENCE Programme Bachelor of Science Branch/Spec. Microbiology

Semester III Version 1.0.1.0

Effective from Academic Year 2016-17 Effective for the batch Admitted in July 2015

Subject code UMBA 301 MBP

Subject Name MICROBIAL PHYSIOLOGY


(Per week) Lecture(DT) Practical(Lab.) Total CE SEE Total

L TU P TW

Credit 03 -- -- -- 03 Theory 40 60 100

Hours 03 -- -- -- 03 Practical -- -- --

Pre-requisites:

Students should have basic knowledge of Metabolism of Biomolecules of 10+2 level.

Learning Outcome:

The course will help the student to understand basic aspects of cellular metabolism and enzymes. In addition students will be able to know about the nutrition and growth of microbes.

Theory syllabus

Unit Content Hrs

1 INTRODUCTION TO BIOMOLECULES AND METABOLISM 1.1. Bio-molecules: Chemical structure, properties, classification and biological significance of

carbohydrates, proteins, lipids and nucleic acids. 1.2. Introduction to modes of microbial metabolism: Anabolism, catabolism, primary and

secondary metabolism. 1.3. Role of reducing power, precursor metabolites and energy rich compounds in cell

metabolism.

15

2 ENZYMES 2.1. General introduction: Physical and chemical properties, Structure of enzymes: Prosthetic

group, apoenzyme, coenzymes, cofactors. 2.2. Localization of enzymes: Extra cellular and intra cellular. Nomenclature and classification of

enzymes, IUB system of enzyme classification. 2.3. Enzyme action: Active sites of enzymes, Mechanism of enzyme action. 2.4. Factors affecting enzyme activity, Inhibition of enzyme activity: Competitive and

noncompetitive.

15

3 MICROBIAL NUTRITION 3.1. Modes of nutritional uptake in microorganisms and their nutritional requirements. 3.2. Classification of microorganisms based on energy source, carbon source and environmental

factors such as oxygen, temperature, pH, osmotic pressure, salt and hydrostatic pressure. 3.3. Utilization of complex nutrients. 3.4. Nutrient transport mechanisms: Primary and secondary transports, simple diffusion and

facilitated diffusion, active transport and synport, group translocations.

15

4 MICROBIAL GROWTH 4.1. Modes of cell division in microorganisms. 4.2. Mathematical nature and expression of microbial growth: growth rate, generation time,

generation number. 4.3. Normal growth curve of microbial population, practical applications of growth curve,

15

Continuous culture and growth of microorganisms, synchronous growth and it‟s applications.

4.4. Criteria and methods for growth measurement: Total cell count, viable cell count, biomass based methods.

Text Books

1 Pelczar, M.J., Chan, E.C.S. and N.R. Kreig (1993). “Microbiology” 5th Edition, Tata Mc Graw Hill Publishing co. Ltd., New Delhi.

2 Modi H.A. (2014). Handbook of Elementary Microbiology, Shanti Prakashan, Ahmedabad.

Reference Books

1 Ingraham J L, and Ingraham, C L, (2000). Introduction to Microbiology, 2ndedn, Brooks/Cole, Singapore.

2 Black, J. G. (2005). “Microbiology: Principles and Explorations”. John Wiley, U.S.A.

3 D,.Nelson and M.Cox, (2005), “Lehninger’s Principles of biochemistry”, 4th ed., Macmillan worth Publishers.

Note:

Version 1.0.0.0 (First Digit= New syllabus/Revision in Full Syllabus, Second Digit=Revision in Teaching

Scheme, Third Digit=Revision in Exam Scheme, Forth Digit= Content Revision)

L=Lecture, TU=Tutorial, P= Practical/Lab., TW= Term work, DT= Direct Teaching, Lab.= Laboratory work

CE= Continuous Evaluation, SEE= Semester End Examination.

GANPAT UNIVERSITY




Subject code UMBA 302 SWM

Subject Name SOIL AND WATER MICROBIOLOGY



L TU P TW

Credit 03 -- -- -- 03 Theory 40 60 100

Hours 03 -- -- -- 03 Practical -- -- --

Pre-requisites:

Students should have basic knowledge of basic microbiology and environment of 10+2 level

Learning Outcome:

The course will help the student to understand role of soil microbes and about microbiology of drinking as well as waste water.

Theory syllabus

Unit Content Hrs

1 MICROBIOLOGY OF SOIL 1.1. Physicochemical properties of soil, soil as a culture medium. 1.2. Microbial flora of Soil, Role of microorganisms in soil: Mineralization and humus formation. 1.3. Methods of studying soil micro flora: Direct microscopic method, agar plate technique,

enrichment culture technique, and buried slide method, Molecular methods for study of soil microorganisms.

1.4. Microbial interactions and associations in soil: Neutral, Positive (Symbiosis, Mutualism, Syntrophism, Commensalism, Synergism) and Negative (Antagonism, Competition, Parasitism, Predation) associations, Interaction between plant roots and microorganisms: i. Rhizosphere and its significance, ii- Mycorrhiza.

15

2 MICROORGANISMS AS BIOGEOCHEMICAL AGENTS 2.1. Introduction to biogeochemical transformations in soil: Mineralization and immobilization

of elements. 2.2. Rotation of elements in nature:

Nitrogen cycle: Proteolysis, ammonification, nitrification, denitrification and nitrogen fixation. Sulfur cycle: Sulfur oxidation and reduction, Carbon cycle: Degradation of complex organic compounds, carbon dioxide fixation.

2.3. Iron cycle: Iron oxidation and reduction. Phosphorus cycle: Mineralization, immobilization and solubilization of phosphorus.

2.4. Soil fertility: Role of microorganisms in soil fertility, biofertilizers.

15

3 MICROBIOLOGY OF DRINKING WATER 3.1. Natural waters: Sources of contamination, Microbial indicators of faecal pollution: Coliforms

as indicator need for differentiation Methods of differentiation: IMViC test and Elevated temperature test, Microbial indicators other than coliforms.

3.2. Nuisance organisms in water: Slime forming bacteria, iron and sulfur bacteria and algae, Water-borne diseases.

15

3.3. Bacteriological examination of drinking water: Sampling, Quantitative analysis: Standard plate count. Qualitative analysis: Multiple tube fermentation method (presumptive, confirmed and completed test), MPN, membrane filter technique, defined substrate test, P-A (Presence-Absence) test.

3.4. Purification of drinking water: Sedimentation, filtration and disinfection, softening of hard water, use of Reverse Osmosis process.

4 MICROBIOLOGY OF WASTEWATER 4.1. Types of wastewater, chemical and microbiological characteristics of waste water. 4.2. BOD, COD and TOC as indicators of strength of wastewater. Pollution problems due to

disposal of untreated wastewater. 4.3. Methods of wastewater treatment: Primary treatment and secondary treatment.

Principles and role of microorganisms in septic tank, Imhoff tank, trickling filters, activated sludge process, oxidation ponds. Advanced treatment and final treatment. Solid waste processing: Anaerobic sludge digestion and biogas formation, Composting.

4.4. Efficiency of wastewater treatment procedures.

15

Text Books

1 Modi H A., (2013), Soil Microbiology, Aavishkar Publishers, Jaipur.

Reference Books

1 Atlas R M., (1997), Principles of Microbiology. 2nded. Wm. C. Brown Pub., Iowa, USA.

2 Pelczar Jr. M J, Chan E C S, Krieg N R, (1986), Microbiology, 5thed, McGraw- Hill Book Co, NY

3 Alexander M, (1977), Soil Microbiology, 2nded Krieger Publ. Co., Melbourne, FL.

Note:





GANPAT UNIVERSITY




Subject code UPMA 303 PRA

Subject Name PRACTICAL MODULE - III



L TU P TW

Credit 03 -- -- -- 03 Theory -- -- --

Hours 06 -- -- -- 06 Practical -- 100 100

Pre-requisites:

Students should have basic knowledge of qualitative estimation of Biomolecules of 10+2 level and basic techniques in microbiology.

Learning Outcome:

The course will help the student to understand detection of biomolecules. In addition students will be able to know about various biochemical tests used for identification of bacteria and methods of enumeration of bacteria.

Practical content

1. Qualitative analysis of biomolecules: A. Carbohydrates: Iodine test, Molisch’s test, Benedict’s test, Barfoed test, Bial’s test and Saliwanoff’s

test. B. Proteins: Biurate test, Ehrlich‟s test, Glyoxilic acid test, Xanthoproteic test.

2. Determination of absorption maxima of a colored solution (use methylene blue 1:20,000 dilution). 3. Study biochemical reaction of bacteria.

A. Based on carbon source. i. Oxidative and fermentative breakdown of glucose.

ii. Fermentation of sugars and sugar alcohol: glucose, xylose, mannitol, lactose, maltose and sucrose. iii. Glucose breakdown product: Methyl red test, Voges-Proskauer’s test. iv. Citrate utilization test. v. Starch utilization test.

vi. Lipid utilization test. B. Based on nitrogen source.

I. Indole production test. II. H2S production test. III. Urea utilization test. IV. Casein hydrolysis test. V. Gelatin hydrolysis test. VI. Deamination test.

C. Other tests. I. Catalase test. II. Dehydrogenase test. III. Oxidase test. 4. Microbiological analysis of soil.

A. Enumeration of organisms from soil (standard plate count-Soil). B. Isolation of symbiotic & non-symbiotic nitrogen fixing bacteria & actinomycetes from soil.

5. Microbiological analysis of drinking water. A. Standard plate count of drinking water. B. Determination of MPN of coliforms in water C. Detection of fecal pollution of water by performing presumptive test, confirmed test and completed

test.

6. Determination of DO from the water sample.

Text Books

1 Experimental Mirobiology Vol. 1 & 2, Rakesh Patel.

Reference Books

Note:





GANPAT UNIVERSITY

FACULTY OF SCIENCE Programme BACHELOR OF SCIENCE Branch/Spec. MICROBIOLOGY



Subject code UCHA 301 IPC Subject Name INORGANIC AND PHYSICAL CHEMISTRY-III



L TU P TW

Credit 03 -- -- -- 03 Theory 40 60 100

Hours 03 -- -- -- 03 Practical -- -- --

Pre-requisites:

Before learning inorganic and physical chemistry, student should aware about basic principles and theories of physical chemistry, thermodynamics, chemical reactions, inorganic compounds, periodic table and other UG level chemistry.

Learning Outcome:

Basic knowledge of wave mechanics.

Knowledge of Nobel gases and their properties.

Understanding of bonding and geometry of xenon complexes.

Knowledge of various theories and equations in thermodynamics.

Understanding of physical properties.

Measurement of surface tension and viscosity.

Theory syllabus

Unit Content Hrs

1

Wave Mechanics

Black body Radiation &Quantum Theory. Photo Electric effect: Wave particle duality of radiation. Compton effect. Basic Postulates of Quantum Mechanics. Operator : Definition, Algebra of operators, Addition, Multiplication, Commutative properties, Linear operator, Commutator operators, Laplassian operator. Free particle system. Particle in one dimension box.

02

Chemistry of Nobel Gases

Introduction. Discovery of Noble gases: Occurrence, Isolation of Non-radioactive of Noble gases. Electronic configuration of Noble gases. Compound of Noble Gases.

1. Non real compounds prepared by different methods. 2. True Compounds : XeF2, XeF4 , XeF6 , XeOF2, XeO3, XeO2F2, XeO4, XeOF4

03

Thermodynamics

Phase in Equilibrium

Clapeyron-clausius equation.

Integrated form of Clapeyron-clausius equation. Application of Clapeyron-clausius equation from various phase in equilibrium Trouton‟s law. Craft equation. Elevation in boiling point (Kb). Depression of freezing point (Kf). Numericals.

04

Physical Properties and Molecular Structure

The Vacancy Theory of Liquid, Vapor-Pressure. Surface Tension

1. Measurement of surface tension by stalagmometer. 2. Perachore and its applications.

Viscosity Measurement of viscosity by Ostwald-viscometer. Refractive Index

1. Specific refraction. 2. Molar refraction. 3. 3. Measurement of Refractive index by Abbe‟s Refractometer.Numericals.

Practical content

Text Books

1 Quantum Chemistry by R.K. Prasad, Revised IIIrd Edition.

2 Physical Chemistry (Question and Answers) by R. N. Madan, G. D. Tually, S. Chand..

Reference Books

1 Advance Physical Chemistry by Gurdeep Raj.

2 Concise Inorganic Chemistry, J.D. Lee, 4th ed., ELBS Publication.

3 Principal of Physical Chemistry by Puri, Sharma & Pathania.

4 Chemical Thermodynamics by R.P. Rastogy and R.R. Mishra.

5 Essential of Physical Chemistry by B.S. Bahal, Arn Bahal and G.D. Tully.

6 Physical Chemistry by P.W. Atkins.

7 Physical Chemistry by R.A. Alberty and R. J. Silbey

8 Physical Chemistry by G.H. Barrow, 5th ed., Mac Graw Hill, 1998.

9 Physical Chemistry by W.J. Moore, 4th ed., Orient Longmans, 1969.

Note:





GANPAT UNIVERSITY

FACULTY OF SCIENCE Programme BACHELOR OF SCIENCE Branch/Spec

. MICROBIOLOGY



Subject code UCHA 302 OAC Subject Name ORGANIC AND ANALYTICAL CHEMISTRY - IV



L TU P TW

Credit 03 -- -- -- 03 Theory 40 60 100

Hours 03 -- -- -- 03 Practical -- -- --

Pre-requisites:

Before studying organic and analytical chemistry all students have basic knowledge of organic compounds, molecular structure, Molecular orbital theories, basics of analytical chemistry and knowledge related to UG level chemistry.

Learning Outcome:

Basic concept and theories of acids and bases.

Understanding of electrophilic aromatic substitution reactions and their mechanism.

Knowledge of principle, instrumentation and applications of colorimetry and spectrophotometry.

Understanding of the principle of photometry and its uses.

Theory syllabus

Unit Content Hrs

1

Acid-Base Properties

Proton Acids-Bases and Lewis Acids-Bases. Scale of Acidity-Basicity. Factor effecting on acidity and basicity of compounds.

Resonance effect (Drawing resonance structures and the conditions for resonance).

Inductive and electronic effects..

Effect of Hybridization.

Steric effects.

Effect by hydrogen bonding.

02

Electrophillic Aromatic Substitution

Introduction. Effect of Substituent groups. Determination of Orientation. Classification of Substituent groups. Orientation in disubstituent Benzenes. Orientation and Synthesis. Mechanism of Nitration, Sulfonation, Fridal-Kraft alkylation and Helogenation. Electrophillic aromatic substitution (Two Step). Theory of reactivity. Theory of orientation. Electron release via resonance.

03 Colorimetry and Spectrophotometry

Law of absorbance, Visual colorimetric method. Spectrophotometric Instrumentation. Light sources optical system, The wave length Selectors. Accuracy and error in spectrophotometry. Analysis of mixture.

4

Potentiometry

The scope of Potentiometric titration. Graphical methods including Grans plot of selecting end point. Deferential potentiometric titration. Dead stop titration. Ion selective electrode elementary. Glass Electrode.

Practical content

Text Books

1 Textbook of Organic Chemistry by Arun Bahal, B.S. Bahal & S. Chand.

2 Organic Chemistry by Morrision and Boyd. 4th ed., Pearson Education-2003.

Reference Books

1 Organic Chemistry by Pine, Hendriction, Cram and Hammond 4th ed. By P. S. Kalsi.

2 Advance Organic Chemistry by Jerry March.

3 Advance Organic Chemistry by Arun Bahal and B. S. Bahal.

4 Organic Chemistry Vol. I & II by S. M. Mukherji, S. P. Singh & R. P. Kapoor.

5 Reaction mechanism and Reagents in Organic Chemistry by Gurdeep R. Chatwal 4th ed., Himalaya Public House.

6 Concise Inorganic Chemistry, J.D. Lee, 4th Edition, ELBS.

7 Organic Spectroscopy by P.S. Kalsi.

8 Organic Chemistry by I.R. Finar.

9 Analytical Chemistry by G.D. Christian, J. Wiley

10 Fundamental of Analytical Chemistry by D.A. Skoog, D.M. West and F.J. Holler.

11 Analytical Chemistry Principals by J. H. Kennedy, W. B. Saunders.

12 Analytical Chemistry Principals and Techniques by L.G. Hargis, Prentice Hall.

13 Principles of Instrumental Analysis by D. A. Skoogs, J. L. Loary, W.B. Saunders.

14 Qualitative Analysis by R.A. Day, Jr and A.L. Underwood, Pr entice Hall.

Note:





GANPAT UNIVERSITY


. MICROBIOLOGY



Subject code UPCA 303 PRA Subject Name PRACTICAL MODULE : III



L TU P TW

Credit 03 -- -- -- 03 Theory -- -- --

Hours 06 -- -- -- 06 Practical -- 100 100

Pre-requisites:

Before performing these practicals, students have basic knowledge of laboratory chemicals, organic compounds and their properties, theories related to conductivity, apparatus and instruments which are used in performing chemistry practicals.

Learning Outcome:

Separation of binary mixture of organic compounds.

Practical knowledge of conductometric titration.

Practical knowledge of pH meter and refractrometere.

Determination of viscosity, specific refraction, molar refraction and dissociation constant.

Practical syllabus

Organic Chemistry

Separation of Organic Mixture. Mixture containing Two Compounds (Only water insoluble solid compounds taken)

Physical Chemistry

1. Conductometric Titration : HCl / CH3COOH Vs NaOH. 2. Conductometric Titration : HCl / NH4OH. 3. pH metric titration:

a. Calibration of pH metry by 4- pH buffer. b. HCl Vs NaOH

4. Determine the Dissociation constant of the acid of the acid of mixture of CH3COONa and CH3COOH by determine the pH.

5. Determine the specific refraction and molar refraction of the given liquid A,B and mixture C (A + B) and calculate the percentage composition of A and B in the mixture C by Abbe‟s Refractrometer.

6. Determine the molar refraction CH3COOC2H5, CH3COOC3H7 and CH3COOC4H9 and show the constancy of reaction equivalent of –CH2 group by Abbe‟s Refractrometer.

7. To determine the viscosity of a different mixture of liquid A and B and determine the percentage composition of unknown mixture by graphical method.

Practical content

Text Books

1

Reference Books

1

2

Note:





GANPAT UNIVERSITY


. MICROBIOLOGY



Subject code UDMB 301 DMT

Subject Name DISASTER MANAGEMENT- I



L TU P TW

Credit 02 -- -- -- 02 Theory 40 60 100

Hours 02 -- -- -- 02 Practical -- -- --

Pre-requisites:

Students should have basic knowledge of Environment and environmental problems.

Students should have acquaintances with natural resources. Learning Outcome:

Knowledge of Natural Disasters.

Understanding of seismic engineering.

Development of thinking to take necessary actions during disaster.

Theory syllabus

Unit Content Hrs

1 Natural Disasters 15

1.1 Types of Natural Disasters: Cyclone, Flood, Fire, Desert Storms, Land Slides and Snow avalanches.

1.2 Cyclone: Introduction, Fundamentals, Characteristics, Causes and Effects, Preventive and Remedial measures.

1.3 Flood: Introduction, Fundamentals, Causes and Effects, Preventive and Remedial measures. 1.4 Fire: Fundamentals, Causes and Effects, Preventive and Remedial measures.

2 Fundamentals of Seismic Engineering 15

2.1 Introduction, Definition, History of earthquake, Earth and its structure, Terminology, Epicentre, Hypocenter, Focus, Epicentre distance 2.2 Waves generated due to earthquake, P waves, S waves, Causes and Measurement of earthquake, Intensity and magnitude of earthquake, Sysmo-graph, Sysmo-scope, Sysmo-meter, Richter scale 2.3 Zoning of earthquake as per I.S. Effects of earthquake on Soil, Low-rise and high-rise buildings, Human psychology, Communication, Geology 2.4 General instructions for protection of people during earthquake. General guidelines for construction and maintenance of earthquake proof/resistant masonry structure

Practical content

Text Books

1

Reference Books

1 Disaster Management By G.K. Ghosh,A.P.H. Publishing Corporation.

2 Disaster Management By R.B. Singh, Rawat Publications.

3 Disaster Management: Through the New Millennium By Ayaz Ahmad, Anmol Publications.

4 Emergency Medical Services and Disaster Management: A Holistic Approach By P.K. Dave Jaypee Brothers Medical Publishers (P) Ltd.

5 Disaster Management By B Narayan, A.P.H. Publishing Corporation.

6 Modern Encyclopaedia of Disaster and Hazard Management By B C Bose, Rajat Publications.

7 Disaster Management By Nikuj Kumar, Alfa Publications.

8 Disaster Management - Recent Approaches By Arvind Kumar, Anmol Publications.

9 Tsunamis: Threats and Management by Dr. Jagbir Singh, I.K. International.

10 Disaster Management Future Challenges and Opportunities by Dr. Jagbir Singh. , I.K. International.

11 Citizen’s guide to disaster management by Satish Modh Publisher:-Macmillan Publishers India.

12 Environment and Sesmic Engineering By Atul Prakashan Ahmedabad.

Note:





GANPAT UNIVERSITY


. MICROBIOLOGY



Subject code UENA 301 ENG Subject Name ENGLISH - III



L TU P TW

Credit 02 -- -- -- 02 Theory 40 60 100

Hours 02 -- -- -- 02 Practical -- -- --

Pre-requisites:

Students should have basic knowledge of English Language and grammar.

Students should have ability to speak and write correct sentences in their day to day language.

Students should be familiar with correct usage of language.

Learning Outcome:

Knowledge of English grammar.

Understanding of prose and composition.

Development of communication skills.

Development of vocabulary.

Understanding communication for HR purpose.

Theory syllabus

Unit Content Hrs

1 Selected Prose 8

The Post Master- Rabindranath Tagore A cup of tea- Katherine Mansfield Blow up with the ship- Wilkie Collins

2 Basic English Grammar 7

Use of relative pronouns, Synthesis of the sentences, Noun clause

3 Vocabulary 7

Confusing words, compound words

4 Composition 8

Joining Letter, Resignation Letter Retirement Letter, Promotion Letter, Reference Letter

Practical content

Text Books

1

Reference Books

1

Note:





Documents

GANPAT UNIVERSITY FACULTY OF SCIENCE TEACHING AND ... · A. Carbohydrates: Iodine test, Molisch’s test, enedict’s test, arfoed test, ial’s test and Saliwanoff’s test. B. Proteins: