FluoGeneTHE FLUORESCENCE PCR certified automation for molecular HLA-, HPA- and RBC-Typing

Ready Gene

PCR & Electrophoresis

SuBiTo

Ready Plate

FluoGene

DNA-Extraction

Software

Life Science

FluoGene is a unique method for molecular HLA-, RBC- and HPA typing combining all advantages of the SSP –PCR with the speed of endpoint fl uorescence detection. The analysis is based on a specifi cally modifi ed TaqMan® probe system by inno-train.

Detection with integrated evaluation is realized in the semi-automated FluoVista version by the FluoGene evaluation software. By the use of a barcode scanner plate-specifi c data can be easily read from the FluoGene tray and sample-ID can be scanned and automatically transferred into the FluoGene Software. The fully-automated FluoStation version with an integrated pipetting unit is already equipped with a barcode scanner.

FluoGene is a self-contained system comprising the ready-to-use Kits, the detection device and the software solution displaying the typing results immediately after the evaluation read. For the PCR set-up simply add your diluted sample DNA to the ready-to-use FluoMix and dispense 15 µl per well over the PCR plate. This manual step is performed automatically by the FluoStation version. Afterwards the FluoGene plates are sealed with an adhesive optical foil. The subsequent pre- and post-reads are generated in a closed environment. Therefore any risk of a post-PCR contamination gets eliminated.

The FluoGene Software supplies the immediate display of typing results, as well as a fully-automated data management.

TECHNIQUE

WORKFLOW KIT COMPONENTSAdd DNA & H2O to FluoMix

Dispense 15 µl per well

Seal plate with optical foil

pre-read

PCR

post-read

Automated data evaluation

ADVANTAGES OF THE FluoGene SYSTEM• from DNA to typing in 90 minutes• PCR-SSP method without gel electrophoresis• no hybridization and washing steps• display of typing results immediately after the post-read• no risk of post-PCR contaminations• low DNA consumption, i.e. 780 ng per HLA-ABDR typing• user-friendly software solution, fully-automated calculation of results• new software feature for RBC-typing: display of genotypes and predicted phenotypes• same system for HLA-, RBC- and HPA• certifi ed for IvD use

• 96 well FluoGene plates with dried primer probe mixes • Ready-to-use FluoMix (Taq, dNTPs and buffer) • Optical foils for sealing the PCR plates

The FluoGene System can be easily upgraded in its degree of automation. In the minimal confi guration our economically-priced FluoVista Analyzer is needed for the fl uorescence detection in addition to the standard thermal cycler. The FluoStation offers an even higher grade of automation by its integrated pipetting unit and barcode scanner in addition to the incorporated

fl uorescence detector. Both systems are delivered with a computer system and the FluoGene Software package.

A modular automation concept from setup to analyzer control to the point of a complete walk-away-automation is in evaluation. For further information please contact us directly.

The supplied FluoGene software automatically combines raw data from pre- and post-read and immediately performs the evaluation of typing results. Positive reactions are clearly displayed with a (+), while negative reactions are shown with a (-). The measured values for the specifi c and control reaction as well as the corresponding cut-off values can be seen by clicking on the bars in the graphical data display.

Further functions are the display of the serological equivalents and the discrimination between CWD and rare alleles.

The FluoGene Software has its own database, saves or exports the results with the corresponding sample information and enables a GMP conform report with a technical and a following medical validation.

VARIABLE DEGREE OF AUTOMATION

EASY EVALUATION BY OUR SMART SOFTWARE SOLUTION

FluoVista FluoStation

FluoGene

006 010 000006 010 010006 010 00S002 07K F10002 07F A01002 07C P01

FluoVista System, incl. computer system and software packageFluoStation System, incl. computer system and software packageFluoGene Calibration System, suffi cient for 10 calibrationsOptical Foils, 10 foils for sealing FluoGene platesFluoApp, applicator for pressing the optical foil against the FluoGene plateFluoPad, compression pad for PCR

ARTICLE NO. ARTICLESYSTEMS AND ACCESSORIES

ARTICLE NO. ARTICLE REACTIONS/TEST TESTS/PLATE TESTS/KIT002 070 010 HLA-FluoGene A 24 1 10002 071 020 / 10 HLA-FluoGene B 48 1 20 / 10002 072 010 HLA-FluoGene C 24 1 10002 073 010 HLA-FluoGene ABC 96 1 10002 074 010 HLA-FluoGene ABDR 96 1 10002 075 010 HLA-FluoGene DR 24 1 10002 079 020 / 10 HLA-FluoGene DPB1 48 1 20 / 10002 077 030 / 10 HLA-FluoGene DRDQ 32 3 / 1 30 / 10002 080 010 HLA-FluoGene DRDQDP 80 1 10002 078 096 HLA-FluoGene B27 1 96 96

ARTICLE NO. ARTICLE REACTIONS/TEST TESTS/PLATE TESTS/KIT003 010 048 / 10 HPA-FluoGene

HPA-1 a/b, -2 a/b, -3 a/b, -4 a/b, -5 a/b, -6 a/b, -9 a/b, -15 a/b9 6 / 1 48 / 10

003 011 048 / 96 HPA-FluoGene HPA-1 a/b Screen 1 12 / 96 48 / 96

ARTICLE NO. ARTICLE REACTIONS/TEST TESTS/PLATE TESTS/KIT001 086 040 / 10 RBC-FluoGene ABO basic

01, 02, B, A, A2 17 4 / 1 40 / 10

001 081 030 / 10 RBC-FluoGene vERYfyRHD: exons 1, 5, 10, psi; RHCE: C, CW, c, E, e; KEL1(K), KEL2(k), JK1(Jka), JK2(Jkb), FY1(Fya), FY2(Fyb), FYnull(Fya-, Fyb-), FYX(Fybweak), MNS1(M), MNS2(N), MNS3(S), MNS4(s), DO1(Doa), DO2(Dob)

32 3 / 1 30 / 10

001 082 040 / 10 RBC-FluoGene CDERHD: exons 1-7, 9, 10, psi, DNB, D cat VII, DHMi, DAU; RHCE: C, CW, c, E, e. Nuevos: RhCE-733C>G, RhCE-1006G>T

24 4 / 1 40 / 10

001 083 048*/ 10* RBC-FluoGene D weak/variantweak D type 1, 1.1, 2, 3, 4.0/ 4.1, 4.2 (DAR), 5, 11 (M295I), 14, 15, 17, K409K, IVS3+1G>A

16 6 / 1 48 / 10

001 085 030 / 10 RBC-FluoGene RareDI1(Dia), DI2(Dib), DI3(Wra), DI4(Wrb), YT1(Yta), YT2(Ytb), LU1(Lua), LU2(Lub), KEL3(Kpa), KEL4(Kpb), KEL6(Jsa), KEL7(Jsb), CO1(Coa), CO2(Cob), KN1(Kna), KN2(Knb)

32 3 / 1 30 / 10

001 087 048 RBC-FluoGene D-ScreenFor screening of RHD exons 3/5 and 10 in a single reaction.

1 12 48

001 088 048 RBC-FluoGene Vel-ScreenFor screening of Vel+ and Vel-.

2 12 48

001 089 010 RBC-FluoGene KKDKEL1(K), KEL2(k), JK1(Jka), JK2(Jkb), FY1(Fya), FY2(Fyb), FYnull(Fya-, Fyb-), FYX(Fybweak)

14 1 10

001 090 010 RBC-FluoGene MNSMNS1(M), MNS2(N), MNS3(S), MNS4(s)

7 1 10

Niederhöchstädter Straße 62 D-61476 Kronberg/Taunus Germany Q

MS

04.14

HLA-FluoGene KITS LOW RESOLUTION

RBC-FluoGene KITS

*The purchase price of this product includes limited, non-transferable rights under European Patent EP 1 047 777 B1.

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HPA-FluoGene KITS

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www.dlongwood.come-mail: dlw@dlongwood.com

Tel. 976 320 638 Fax. 976 320 660

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