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DAFTAR ISI

HalamanHALAMAN JUDUL........................................................................................... i

HALAMAN PERSETUJUAN............................................................................ ii

MOTTO DAN PERSEMBAHAN...................................................................... iiiABSTRAK.......................................................................................................... iv

ABSTRACT........................................................................................................ v

KATA PENGANTAR.......................................................................................... vi

DAFTAR ISI....................................................................................................... viiiDAFTAR TABEL................................................................................................ x

DAFTAR GAMBAR.......................................................................................... xiDAFTAR LAMPIRAN....................................................................................... xii

BAB I PENDAHULUAN

1.1....................................................................................................LataB!la"an# ................................................................................... 1

1.$....................................................................................................R%m%&

an Ma&ala'................................................................................ (1.)....................................................................................................T%*%an

P!n!litian................................................................................... (

1.(....................................................................................................Man+a

at P!n!litian............................................................................... (

BAB II LANDASAN TEORI

$.1. Tin*a%an P%&ta"a....................................................................... ,A. Ta-a" Daa Catharanthus roseus/.................................... ,

B. E"&ta"&i Ma&!a&i............................................................. 0

C. Antia"t!i......................................................................... 2D. U*i A"tivita& Antia"t!i.................................................... 13

E. Ba"t!i U*i.......................................................................... 11

$.$. K!an#"a P!mi"ian.................................................................. 1)

A. K!an#"a T!4i................................................................... 1)

B. K!an#"a K4n&!-............................................................... 1($.). Hi-4t!&i&.................................................................................... 1(

BAB III METODE PENELITIAN

).1. J!ni& P!n!litian.......................................................................... 15

).$. T!m-at 6an 7a"t% P!n!litian.................................................... 15).). Ja68al P!n!litian....................................................................... 15


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).(. Ba'an......................................................................................... 1,

).5. P!alatan P!n!litian................................................................... 1,

).,. D!&ain P!n!litian....................................................................... 10).0. P!m%atan E"&ta" Da%n C. roseus.......................................... 10

).2. P!m%atan M!6ia...................................................................... 12).9. U*i A"tivita& Antia"t!i........................................................... 19

).13. P!n#%"%an :4na Hamat....................................................... $3

).11. I6!nti+i"a&i ;aia!l................................................................. $3

).1$. Anali&i& Stati&ti"...................................................................... $1

BAB I; HASIL DAN PEMBAHASAN

(.1. E"&ta"&i Da%n C. roseus.......................................................... $$(.$. P!n!nt%an A"tivita& Antimi"4a.............................................. $$

BAB ; KESIMPULAN DAN SARAN5.1. K!&im-%lan............................................................................... $9

5.$. Saan.......................................................................................... $9

DAFTAR PUSTAKA

LAMPIRAN


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BAB I

PENDAHULUAN

1.1 Latar Belakang

In64n!&ia m!%-a"an n!#aa am -!naa ta6i&i4nal 6a-at 6i-!tan##%n# *a8a"an

-!n##%naann


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tanaman?tanaman t!&!%t 6a-at 6i*a6i"an &!>aa alt!nati+ 4at?4atan "4m!&ial

Nia= $330/.

Um%mn

-!naan#. Batan# %lat= a#ian -an#"al !"ain#= t!-i ata= -!t%lan#an m!n


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B%a'ni&tin! aa

n4mal 6i 6alam 'i6%n# 6an -a6a "%lit &!"ita $5 ?)3 6ai 4an# 6!8a&a


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m!n

E. coliia&an

m!n

ata% m!lal%i "4nta" 6!n#an '!8an ata% 4an# CDC= $31$/. G!*ala -!naa In ;it4

1. Tu!uan Penel"t"an

T%*%an %m%m -!n!litian ini a6ala' 6a-at m!li'at 6an m!n#!ta'%i a6an


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'amat >aa 6i&" 6i+%&i 6i %"% 6!n#an -!n##ai&= t!'a6a- 6a


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BAB II

LANDASAN TE%RI

2.1 TIN&AUAN PUSTA'A

A. Ta(ak Dara )Catharanthus roseus*

Ta-a" 6aa Catharanthus roseus/m!%-a"an tanaman '!a&!ma" il=

!"aaan#= 6an !am%t &an#at l!at. Da%n !&%&%n !'a6a-an=

!tan#"ai -!n6!"= m!man*an# %lat t!l% 6!n#an -an#"al &!%-a a*i 6an %*%n#

t%m-%l -an*an# $, >m= l!a 1) >m= 6an tan#"ai 6a%nnil N!8i!= $31)/.

G. 1.1. T%m%'an C. roseus

Kla&i+i"a&i C. roseus

6
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Kin#64m PlantaeT%m%'an/

S%"in#64m Tracheobionta T%m%'an !-!m%l%'/

Divi&i MagnoliophytaT%m%'an !%n#a/

S%-! Divi&i SpermatophytaM!n#'a&il"an i*i/

K!la& Magnoliopsida!"!-in# 6%a 6i"4til/

S% K!la& Asteridae

O64 Gentianales

Famili Apocynaceae

G!n%& Catharanthus

S-!&i!& Catharanthus roseus(L.

K4m-4n!n anti"an"!=

6!n#an m!n##%na"an -!la%t t!t!nt%. E"&ta"&i t!#ant%n# -a6a t!"&t% 6an

"an6%n#an a'an 6alam t%m%'an. S!n


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!tila&!tat. E"&ta"&i ia&anaa !ta'a- 6im%lai 6!n#an -!la%t


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a/. Antia"t!i

S!n" 6"".= 1993/. Ban


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M!n%%t N4via $331/= -!n#%*ian 6aa8an

-!ti "!m%6ian m!n!m-at"an &%at% >a"am "!ta&


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-a6a -!m%"aan m!6ia t!&!%t. S!t!la' ma&a in"%a&i t!t!nt%= 6iamati %nt%"

m!li'at a6anam m!6ia= in4"%l%m

6an la*% 6i+%&i a'an antimi"4a.

Met/eDisc Difussion )tes '"r,0 /an Bauer*

M!t46! ini %nt%" m!n!nt%"an a"tivita& a#!n antimi"4a. Piin#an


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G. 1.$. S. aureus

S. aureusm!%-a"an -!n

-!nim%nan nana' a"iat in+!"&i a"t!i/= &!6an#"an -a6a '!8an S.aureus

m!n

-4&t%la 6!matiti& a6an# "%lit/ -a6a an*in#= &!ta a&!& -a6a %n##a& N!8i!=

$33$/.

,*.Escherichia coli

E. colim!%-a"an -!n#'%ni %&%& !&a man%&ia 6an '!8an tin#"at tin##i

lainn@a 6an C'an 192,/.

12


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G. 1.).E. coli

E. colit!ma&%" "!6alam +amiliEnterobacteriaceae= m!%-a"an a"t!i #am

n!#ati+= !&i+at ana!4 +a"%ltati+. Ba"t!i ini !!nt%" atan# -!n6!" 6!n#an l!a

"%an# 6ai 1=1?1=5 m 6an -an*an# &!"ita $=3?,=3 m= nilai -H 4-tim%mn


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B. 'erangka 'nse(

2. HIP%TESIS

Hi-4t!&i& 6ai -!n!litian ini a6ala'

a. E"&ta" 6a%n C. roseusmam-% m!n#'amat -!t%m%'an ata% m!mati"an

a"t!i S. aureus6an E. coli.

b. E"&ta" 6a%n C. roseus ti6a" mam-% m!n#'amat -!t%m%'an ata%

m!mati"an a"t!i S. aureus6an E. coli.

BAB III

14

Antia"t!i

H!al

Staphylococcus

aureus

P!t%m%'an

6i'amat ata%

ti6a"

E"&ta" 6a%n

C. roseus

U*i A"tivita& Antia"t!i

M!t46!"isc "i$ussion


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MET%DE PENELITIAN

.1 &en"s Penel"t"an

J!ni& -!n!litian


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5 P!&ia-an alat 6an

a'an

, P!n#%m-%lan Data

0 Anali&i 6ata 6an

-!ma'a&an

2 K4n&%lta&i la-4an

-!n!litian

9 P!la-4an Ha&il

P!n!litian

.# Bahan

Ba'an


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m!n##%na"an %*i 6i+%&i >a"am dis# di$$usion test/. M!t46! %*i 6i+%&i >a"am dis#

di$$usion test/ m!%-a"an &ala' &at% m!t46! aa &!an

&am-ai ( 'ai "!m%6ian 6i&ain#= -!la"%an ini 6ila"%"an &!lama ) 'ai. S!lan*%tn


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F4m%la&i m!6ia NA DIFCO -! lit! a6ala' 5 # a>t4 -!-t4n= ) #am bee$

e&tract= 5 # NaCL= 6an 15 # a>t4 a#a. S!an6an al%mini%m +4il. M!6ia ini 6i&t!il"an m!n##%na"an 4t4"la+ -a6a t!"anan 1=5

Atm= 1$14C &!lama 15 m!nit.

Me/"a Muller H"ntn Agar )MHA*M!6ia MHA 6!n#an "!t!alan "%an# l!i' , mm 6i%at 6!n#an >aa

m!niman# &t4" "!m%6ian 6ila%t"an 6alam a%a6!& &amil 6i-ana&"an 6an

6i6i&ti%&i"an -a6a ta%n# 6!n#an "!t!alan a8an -!ti &t!il 6!n#an 6iam!t! 6an m!"


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&!lama $( *am. Bia"an ini m!%-a"an ia"an a8al a"t!i 6iin"%a&i 6alam in"%at4 !#4a"am dis#

di$$usion test/ 6!n#an ) "ali -!n#%lan#an 6an &!a#ai -!man6in# 6i#%na"an

T!ta&i"lin )3 #6i&". E"&ta" 6a%n C. roseus 6i!n>!"an 6!n#an a%a6!& &!'in##a

m!n*a6i "4n&!nta&i 133= 53= $5= 1$=5 6an ,=$5 6an "!m%6ian ma&in#?ma&in#

"4n&!nta&i !"&ta" 6ima&%""an 6alam ta%n# !a"&i. S!ana"am

"4&4n# !%"%an , mm a"am t!&!%t

"!m%6ian in"%a&i -a6a &%'% )04C &!lama 12 ? $( *am.

.14 Pengukuran :na Ham,at

19


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P!n#%"%an @4na 'amat 6!n#an >aa 6i&" 6i+%&i 6i%"% 6!n#an -!n##ai&.

Hamatan a"an t!li'at &!a#ai a!a


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independent. U*i ini 6i#%na"an %nt%" m!n#!ta'%i a6a ata% ti6a"n


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BAB. I;

HASIL DAN PEMBAHASAN

4.1 Ekstraks" Daun C. roseus

Ha&il !"ta"&i 133 # &!%" 6a%n C. roseus6!n#an 0533 ml !tan4l 9, 6i-!4l!'

!"&ta" -a6at !8ana 'i*a% -!"at &!an


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D"ameter Daerah Ham,at )DDH* ,akter" )mm*

Bakter" u!" Ulanga

n

144< 54< 25< 12=5< 6=25< Tetras"kl"n

$ $1 19 10 1( 2 $)

) $$ 12 10 11 9 $)

( $$ 12 10 1) 9 $)

5 $1 19 12 11 13 $)

, $$ 19 12 1) 2 $)

Stan/ar De-"as" 4=5#8 4=75 4=5#8 1=225 4=89#

Ga+i" Diam!t! Hamat Pa6a Ba"t!i Staphylococcus aureus

1 2 3 4 5 6

0

5

10

15

20

25

30

35

100%

50%

25%

12,50%

6,25%

Ulangan

23


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Ga+i" Diam!t! Hamat Pa6a Ba"t!iEscherichia coli

1 2 3 4 5 6

0

5

10

15

20

25

30

35

100%

50%

25%

12,50%

6,25%

Ulangan

Unt%" m!n#%*i 6a


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Dai 'a&il t!& K4lm4#44v?&min4v 6i6a-at"an nilai &i#ni+i"an&i l!i' !&a

6ai nilai - val%! 3=35/ &!'in##a 6in


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6!via&i 3=5(2= "4n&!nta&i !"&ta"&i 1$=5 nilai ata?ata @4na 'amat a6ala' 1$=53

6!n#an &tan6a 6!via&i 3=1=$$5 6an "4n&!nta&i !"&ta"&i ,=$5 nilai ata?ata @4na

'amat a6ala' 9=33 6!n#an &tan6a 6!via&i 3=29(.

S!6an#"an 6ai 'a&il %*i independent samples test)-a6a "4n&!nta&i !"&ta"&i

133 6i-!4l!' nilai &i#ni+i"an&i 3=333 3=35= *a6i -a6a "4n&!nta&i !"&ta"&i 133

!"&ta" 6a%n C. roseusmam-% m!n#'amat -!t%m%'an ata% m!mati"an a"t!i S.

aureus6an E. coli. Pa6a "4n&!nta&i !"&ta"&i 53 6i-!4l!' nilai &i#ni+i"an&i 3=333

3=35= *a6i -a6a "4n&!nta&i !"&ta"&i 53 !"&ta" 6a%n C. roseus mam-%

m!n#'amat -!t%m%'an ata% m!mati"an a"t!i S. aureus 6an E. coli. Pa6a

"4n&!nta&i !"&ta"&i $5 6i-!4l!' nilai &i#ni+i"an&i 3=333 3=35= *a6i -a6a

"4n&!nta&i !"&ta"&i $5 !"&ta" 6a%n C. roseusmam-% m!n#'amat -!t%m%'an

ata% m!mati"an a"t!i S. aureus 6an E. coli. Pa6a "4n&!nta&i !"&ta"&i 1$=5

6i-!4l!' nilai &i#ni+i"an&i 3=333 3=35= *a6i -a6a "4n&!nta&i !"&ta"&i 1$=5

!"&ta" 6a%n C. roseusmam-% m!n#'amat -!t%m%'an ata% m!mati"an a"t!i S.

aureus6an E. coli. Dan -a6a "4n&!nta&i !"&ta"&i ,=$5 6i-!4l!' nilai &i#ni+i"an&i

3=333 3=35= *a6i -a6a "4n&!nta&i !"&ta"&i ,=$5 !"&ta" 6a%n C. roseusmam-%

m!n#'amat -!t%m%'an ata% m!mati"an a"t!i S. aureus6an E. coli.Pa6a !!a-a -!n!litian= t!la' 6ila-4"an a'8a a"t!i #am -4&iti+

6it!m%"an l!i' &!n&iti+ 6ai-a6a a"t!i #am n!#ati+. Hal ini 6a-at t!*a6i "a!na

a"t!i #am n!#ati+ m!mili"i m!man l%a= &!m!ntaa a"t!i #am -4&iti+ 'an!n6!%n# l!i' &!n&iti+ t!'a6a-

antia"t!i= "a!na &t%"t% 6in6in# &!l a"t!i #am -4&iti+ l!i' &!6!'ana

6ian6in#"an &t%"t% #am n!#ati+ &!'in##a m!m%6a'"an &!n


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%nt%" ma&%" "!6alam &!l a"t!i #am -4&iti+. S!&%ai 'a&il -!n!litian

-!mi&a'an &!n!n6!%n# m!nin#"at &!an6in#

6!n#an m!nin#"atn


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n!#ati+. Ba"t!i #am n!#ati+ l!i' an

&!n

6!n#an &!n


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t!'a6a- !!a-a a"t!i= 4l!' "a!na it% -!l% 6i>ai alt!nati+ -!n##anti -!ma"aian

antii4ti" 6!n#an 4at ta6i&i4nal.

P!n!litian ini m!n##%na"an antii4ti" t!ta&i"lin &!a#ai -!man6in# "a!na

t!ta&i"lin t!ma&%" antii4ti" !&-!"t%m l%a& aa m!n#'amat &int!&i&

-4t!in a"t!i P!l!@a 6an C'an= 1922/. Diam!t! 6a!a' 'amat antii4ti"

t!ta&i"lin )3 #6i&" m!n%%t NCCLS Nati4nal C4mmitt!! +4t Clini>al La4at4" JL= A6!l!# EA 199,/.Mi#robiologi ,edo#teran Edisi #e/07.Ja"ata K!64"t!an EGC.

Ja8!t@ E= M!lni>" JL= A6!l!# EA $335/.Mi#robiologi 1ntu# Pro$esi ,esehatan.

Ja"ata K!64"t!an EGC.

J4&46i84n64 S= UC 7a&a= A S4!'ian6i4 6an P S4!6am4n4 199,/.

Maalah #edo#teran -ndonesia Per#embangan #uman terhadap antimi#robasaat ini. I"atan D4"t! In64n!&ia= (,9/ (,0.

K!nn!t' T46a $33$/. The Control o$ Microbial Gro%th. 7i&>4n&in Univ!&it< 4+7i&>4n&in.

K!nn!t' T46a $31$/. 'nline te&tboo# o$ bacteriology staphylococcus aureus and

s. aureus desease. T'! Am!i>an& S4>i!t< +4 Mi>4i4l4#< E6%>ati4nal

7!&it! Mi>4 74l6. 888.t!xt44"4+a>t!i4l4#


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diagnosis or treatment Staph in$ection (staphylococcus aureus.

M!6i>in!N!t= In>. 888.m!6i>in!n!t.>4m&ta-'?in+!>ti4nati>l!. 'tml.

Dia"&!& M!i $31).

Ma&niai P4!l4!n#an= An6iani= S%&an MN= I

1i "aya Antiba#teri E#stra# Etanol ,ulit !atng !ungur (Largerstoremiaspesiosa Pers Terhadap Staphylococcus aureus "an Escherichia coli Secara

-n *itro. B4#4 Balai B!&a P!n!litian ;!t!in!.

N!8i! T4a $31)/."unia Pertanian Catharantus roseus) ,lasi$i#asi Tanaman)

,lasi$i#asi Tapa# "ara (Catharantus roseus) Mor$ologi) Mor$ologi Tapa#

"ara (Catharantus roseus) Tanaman 9erba) Tanaman 9erba (Catharantusroseus) Tapa# "ara) Tapa# "ara (Catharantus roseus. P!malin".

'tt-888."la&i+i"a&i?6an?m4+4l4#i?tanaman?ta-a"?6aa'Catharantusroseus/?N!8i!?T4a. 'tm.Dia"&!& A-il $31).

Nia In6iani $330/.A#tivitas Antiba#teri "aun Segugu (Clerodindron serratum :L.;

spr. B4#4 Fa"%lta& Mat!mati"a 6an Ilm% P!n#!ta'%an Alam= In&tit%t

P!tanian B4#4.

N4via Da"%ni $331/.Mi#robiologi.Malan# JICA

P!l!@a MJJ= C'an ECS 192,/. "asar/dasar Mi#robiologi. Ja"ata Univ!&ita&

In64n!&ia.

P!l!@a MJJ= C'an ECS 1922/. "asar/dasar Mi#robiologi. Ja"ata Univ!&ita&

In64n!&ia.

Pati8i ST $332/. Mi#robiologi 2armasi.Ja"ata Elan##a.

PaS%li&tia Gani&8ana $331/.2arma#ologi "an Terapi Edisi #e/8.Ja"ataFama"4l4#i FKUI.

34


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S P4na%l&!lvam= C Pann!!&!lvam= K M%%#an= N At'i= K Kalim%t'%= an6 S

T'an#amani $31$/.Asian paci$ic ournal o$ tro$ical biomedicine synthesis

o$ silver nanoparticles using leaves o$ catharontus roseus linn.g. don andtheir antiplasmodial activities. US Nati4nal Lia< 4+ M!6i>in! Nati4nal

In&tit%t!& 4+ H!alt'= $0/ 50(?523.

Ta%n Ma6a--a $31$/."rugs) diseases and procedures e. coli in$ections.

M!6&>a-! R!+!!n>!. 888.!m!6i>in!.m!6&>a-!.>4mati>l!$10(25.

4v!vi!8. Dia"&!& M!i $31).

7a'i6 P 199)/.Medicinical and Aromatic Plant in -ndonesia. Ban#"4" RAPA

P%li>ati4n.

7inan4= Fa6ia@ D= Fa&ia@ S 190)/.E#stra#si) ,romatogra$i) dan Ele#tro$oresis.B4#4 Fa"%lta& T!"n4l4#i P!tanian= In&tit%t P!tanian B4#4.

7HO $31)/.9ealth topics e. coli in$ections.74l6 H!alt' O#anitati4n.

888.8'4.intt4-i>&!&>'!i>'ia>4liin+!>ti4n&. !n. 6ia"&!& M!i $31).

7%lan6ai NDM $335/.Perbandingan Metode E#stra#si !uah Mah#ota "e%a

(Phaleria macrocarpa dan 1i To#sisitas Sub#ronis Pada Ti#us Putih.

B4#4 Fa"%lta& Mat!mati"a 6an Ilm% P!n#!ta'%an Alam= In&tit%t P!tanianB4#4.

35
http://www.emedicine.medscape.com/article/217485http://www.emedicine.medscape.com/article/217485http://www.who.int/topics/escherichia_coli_infections.%20en.%20diakses%20Mei%202013http://www.emedicine.medscape.com/article/217485http://www.who.int/topics/escherichia_coli_infections.%20en.%20diakses%20Mei%202013


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LAMPIRAN 1

+ara Pem,uatan Ekstrak Daun C. roseus

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LAMPIRAN 2

Has"l U!" Ant",akter" Ekstrak Daun C. roseusTerha/a(S. aureus/anE. coli

Se>araIn ;"tr

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