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www.4ti.co.uk
Designed with robotic precision in mind
96 and 384 well PCR plates
Warp free
Within SBS footprint pre and post PCR
Improved seal integrity
Recommended for low volume PCR
Thin-wall design for fast PCR
Reliable use with stackers and liquid handling robots
Guaranteed well integrity
Free of human DNA, RNAses and DNases
Clean-room produced
Bar coding & Colour options
FRAMEstar ®
Benefits
Manufactured under license from Bio-Rad Laboratiories, Inc. U.S. Patent 6,340,589, EP 1198293 B1, and foreign counterparts.
Features and Benefits
Table 1: Plate Distortion post PCR
X-axis Y-axis Warpage
FrameStar® 96, skirted 0.02mm 0.02mm 0.03mm
One Piece 96 well 1.18mm 1.69mm 1.8mmPP Plate
Distortion tests have been performed with different PCR profiles using a variety of sealing materials. The results shown above were obtained using the following protocol with 5µl reaction volumes, plates sealed with adhesive sheets (4titude code 4Ti-0500), in a ThermoHybaid Px2 Cycler: 1min 94ºC, 30x(30sec 94ºC, 30sec 55ºC, 30sec 72ºC), 5min 72ºC, holding step at 4ºC. Averages from measurements of 10 plate samples are shown. X- and Y- axis measurements were taken in the middle sections of the plate skirt. Warpage was measured as a maximum deviation from a flat plane.
4titude®’s FrameStar® PCR plates are
a two-component design combining the
advantages of thin-walled tubes for optimum
PCR results and a rigid skirt and deck for
reliable precision handling.
For the tubes we use polypropylene which provides the
most efficient heat transfer, as well as an inert surface with
low binding capabilities for nucleic acids, proteins and other
molecules. By using novel injection moulding technology
the wall thickness has been reduced without compromising
on tube integrity enabling 4titude to offer a superior
product for fast PCR and small reaction volumes.
By moulding the plate frame in polycarbonate the
mechanical stability of the plate has been greatly improved
compared with traditional single piece products. Warping,
shrinkage and thermal distortion are eliminated, providing
a number of advantages:
Well spacing and position post PCR remain accurate so
that liquid handling devices can reliably add or remove
the smallest quantities from the plate.
Robotic arms can consistently grip and move cycled
FrameStar® plates.
FrameStar® plates will not stick in PCR blocks after PCR
or get jammed in the stacker of robotic instruments.
Improved flatness reduces variation of fluorescent
signals in optical assays such as Q-PCR.
Reduced sample loss due to improved seal integrity
FRAMEstar ®
FrameStar® 384 0.02mm 0.02mm 0.03mm
One Piece 384 well 1.22mm 1.82mm 2.2mmPP Plate
Bar CodingFrameStar® plates are available with bar
coding. Our labels are tested for PCR compatibility
as well as cold storage. They can be applied on any
one of the 4 sides of the plate skirt. Different types
of bar code are available. Please contact 4titude®
or your local distributor to discuss your
individual requirements.
Improved SealingIn addition FrameStar® technology provides a
much-improved level of seal integrity: During PCR,
with either heat seals or adhesive seals, the excessive
thermal expansion/contraction of standard one-piece
polypropylene plates, can have a damaging effect on seal
quality. This is especially evident around the edge of the
plate where these forces are at their maximum resulting
in significant sample evaporation from the outer wells.
In contrast, the higher thermal stability of FrameStar®
plates results in a reliable seal in all areas of the plate
during PCR and in cold storage down to minus 80ºC.
Reduced risk of cross contaminationRaised rims around each tube aid the prevention of
cross contamination between samples. This effect is
enhanced by the 2 component design of the plates:
Capillary effects and the roughened surface of the
plate deck provide additional physical barriers for
sample droplets and prevent them from reaching
adjacent wells.
Fluorescence based AnalysisFor experiments involving fluorescence detection,
e.g. Q-PCR, we have developed plates which are
especially low in autofluorescence. For this type of
experiment we recommend to use our plates with white
wells and black frames: White wells have shown to
maximise signal intensity due to the reflective properties
of the pigment. At the same time cross talk between
adjacent wells is minimised by the black, light absorbing
plate frame and deck. This is a colour combination that
cannot be offered by traditional one piece PCR plates.
FrameStar® plates are produced under clean-room
conditions in the most modern injection moulding
facilities. Particles, bacterial cells and other
contaminants are filtered from the atmosphere so
that FrameStar® plates are kept free from any factors
which may affect the quality of experimental results.
Only virgin polymers are used to avoid the presence
of impurities.
Our stringent QC procedures guarantee 100%
well integrity and the absence of contaminants:
Each individual plate well is robotically tested for holes
Leak tests are performed to test the seal integrity
Biological test ensure the absence of human DNA, nucleases and other PCR inhibitors.
Manufacturing & Quality Control
Sealing Options4titude offers a wide range of sealing options for
FrameStar® plates:
A variety of optical, piercable and peelable heat
sealing options for manual and automated use
(sheets and rolls respectively).
Adhesive films and foils including materials for
Q-PCR and other fluorescence based applications.
Domed (4ti-0752) or flat, optical (4ti-0751) cap
strips for all 96well plates.
Detailed information on all sealing options can be
found on www.4ti.co.uk.
Colour OptionsFrameStar® plates are available with
purple, blue, red, green, clear and black
frames, all with clear tubes. In addition
we offer purple plates with white tubes
for optical assays.
4titude® offers a range of adhesives and heat sealing films and foils. The plate above has been sealed with Clear Adhesive PCR Film, code 4Ti-0500
The plate above shows FrameStar 96 semi-skirted (code 4ti-0730) sealed with flat, optically clear cap strips (code 4ti-0751)
FrameStar® 96 skirted (4ti-0960)The No 1 choice for robotic applications and low volume PCR
FrameStar® 96 non-skirted (4ti-0710)Fits most thermal cyclers
FrameStar® 384 (4ti-0384)Ideal for robotic use
FrameStar® 96 semi-skirted with upstand (4ti-0730)Designed to fit ABI thermal cyclers and sequencers
FrameStar® 96 non-skirted low profile (4ti-0720)Recommended for low volume PCR
FrameStar® 96 and 384Available with white wells for fluoresence applications (see table for catalogue numbers)
FRAMEstar ®The Range
Plate Compatibility Table
FrameStar 96 skirted low profileCode 4ti-0960(clear wells)Code 4ti-0961(white wells)
FrameStar 384 skirtedCode 4ti-0384(clear wells)Code 4ti-0385(white wells)
FrameStar 96 non-skirtedCode 4ti-0710(clear wells)Code 4ti-0711(white wells)
FrameStar 96 non-skirted low profileCode 4ti-0720(clear wells)Code 4ti-0721 (white wells)
FrameStar 96 semi-skirted Code 4ti-0730(clear wells)Code 4ti-0731(white wells)
Thermal Cyclers & Sequencers
STANDARD CYCLERS
ABI
GeneAmp®9600 yes yes
GeneAmp®9700 yes yes yes
Biometra
Uno yes yes yes
T1 Thermocycler yes yes yes yes
TGradient yes yes yes
TRobot yes yes yes
Biorad
iCycler™ yes
MyCycler yes
Biorad-MJ Research
PTC100™ (96well block only) yes yes yes
PTC200 (DNA Engine™) yes yes yes yes
PTC225 (DNA Tetrad™) yes yes yes yes
Corbett Research
Palm Cycler yes yes
Eppendorf
MasterCycler® Gradient yes yes yes
MasterCycler® EP Gradient yes yes yes
M384 yes
ThermoHybaid
PCR Express yes yes yes yes
PX2 / PXE yes yes yes yes
MBS, Multiblock System yes yes yes yes
MWG
Primus 96 yes yes yes
Primus 384 yes
The Q-Lifecycler yes yes yes
Stratagene
Robocycler yes
Takara
TP3000
Techne
TC412 yes yes yes
TC512 yes yes yes
Q-PCR CYCLERS
ABI
PRISM®7000/7300/ yes
7500/7700 yes
PRISM®7900HT yes yes
Biorad
iCycler™IQ
iQ5 yes
MyiQ
Biorad-MJ Research
Opticon™ yes yes
Opticon2™ yes yes
Stratagene
Mx3000™
Mx4000®
Techne
Quantica® yes yes
SEQUENCERS
ABI
PRISM®3100 yes
PRISM®3700 yes yes
PRISM®3730/3730x yes
Amersham
MegaBACE™500 yes
MegaBACE™1000 Mark II yes
MegaBACE™4000 yes
Biorad MJ Research
BaseStartion™ yes
Transgenomic
WAVE® System yes
Plate CompatibilityFrameStar® well plates are compatible with most PCR
cyclers, real-time detection systems and sequencers.
Automation Platforms:
FrameStar® plates have been successfully tested
for reliable use on most automation platforms,
e.g. Beckman, Hamilton, Tecan and Zymark.
Plate Dimensions
FrameStar® 96 well plate skirted
FrameStar® 96 well plate semi-skirted with upstand
FrameStar® 384 well plate skirted FrameStar® 96 well plate non-skirted standard profile
FrameStar® 96 well plate non-skirted low profile
Plate Dimensions
Unit 4bc, Jayes Park Courtyard,
Ockley, Surrey RH5 5RR, UK
Tel: +44 (0)1306 621111
Fax: +44 (0)1306 621162
Web: www.4ti.co.uk
Email: [email protected]
FRAMEstar ®
4Ti-0384
4Ti-0384/B
4Ti-0384/C 4Ti-0384/G 4Ti-0384/R 4Ti-0384/X 4Ti-0385
384 well plate
FrameStar® PCR plate, clear wells, purple frame
FrameStar® PCR plate, clear wells, blue frame
FrameStar® PCR plate, clear wells, clear frame
FrameStar® PCR plate, clear wells, green frame
FrameStar® PCR plate, clear wells, red frame
FrameStar® PCR plate, clear wells, black frame
FrameStar® PCR plate, white wells, black frame
96 well plate
skirted
4Ti-0960
4Ti-0960/B
4Ti-0960/C 4Ti-0960/G 4Ti-0960/R 4Ti-0960/X 4Ti-0961
96 well plate semi-skirted with upstand
4Ti-0730
4Ti-0730/B
4Ti-0730/C 4Ti-0730/G 4Ti-0730/R 4Ti-0730/X 4Ti-0731
96 well plate non-skirted standard profile
4Ti-0710
4Ti-0710/B
4Ti-0710/C 4Ti-0710/G 4Ti-0710/R 4Ti-0710/X 4Ti-0711
96 well plate non-skirted low profile
4Ti-0720
4Ti-0720/B
4Ti-0720/C 4Ti-0720/G 4Ti-0720/R 4Ti-0720/X 4Ti-0721
50 plates per case 50 plates per case 50 plates per case
50 plates per case 50 plates per case 50 plates per case 50 plates per case
Ordering InformationCode Description Quantity
E-mail: rafer rafer.es - www.rafer.es@E-mail: rafer rafer.es - www.rafer.es@
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