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Evolution at the Nucleotide Level. Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans. Matthew D. Pagel NSCORT/Exobiology Fellow Christopher Wills Lab Department of Biology, University of California - San Diego. HIV/SIV Phylogenetic Trees. A Model for Rapid Evolution. - PowerPoint PPT Presentation
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Evolution at the Nucleotide Level
Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans
Matthew D. Pagel
NSCORT/Exobiology Fellow
Christopher Wills Lab
Department of Biology, University of California - San Diego
HIV/SIV Phylogenetic Trees
A Model for Rapid Evolution
Sample Sequence Alignment
HIV/SIV Env gene Neighbor-
Joining Tree(from Yellow Fever, Black Goddess
by C. Wills)
Neighbor-Joining Tree of Env gene of HIV/SIV - NCBI/Los Alamos Complete Sequences
Pol Neighbor-Joining Tree
HIV1
HIV2
Neighbor-Joining
Tree of Gag Gene of HIV/SIV
NJ Tree of Gag after Unverified Sequences
Removed
Cloning of Candida albicans genes involved in pathogenicity
Plasmid Construction of Erk1
Microsatellite Slippage Mutations (Glutamine)
The Genetic Code--The Translation of mRNA to Amino Acids--
Recombination-Mediated Plasmid Construction
Action of Restriction Enzymes(Eco RI example clipped from Molecular Cell Biology CD-Rom)
Plasmid Constructionvia Restriction Digest
Blue/White Screen
Future Steps
• Site-directed mutagenesis producing variant microsatellite length
• Re-insertion of plasmid into knockout strains
• Analysis of morphological form (hyphal)
Acknowledgements
Chris Wills
Nikki Freed
Dave Metzgar
Sol Guiterrez
Hattie Oswald
Lois Lane
Bill Baity
Carlos Flores, Vincent Starai, Carlos Peralta, & William Engels
The NSCORT/Exobiology Program