ENHANCEMENT OF PRECURSOR ION SCANNING ON A Q-TOF … · 2007. 11. 21. · Steve Bajic, Robert H Bateman, Kevin Giles, Steven D Pringle and Jason L Wildgoose Waters Corporation, MS

©2005 Waters Corporation

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ENHANCEMENT OF PRECURSOR ION SCANNING ON A Q-TOF INSTRUMENT USING A TRAVELLING WAVE ION MOBILITY SEPARATOR

Steve Bajic, Robert H Bateman, Kevin Giles, Steven D Pringle and Jason L Wildgoose Waters Corporation, MS Technologies Centre, Manchester, UK

ASMS 2005

Precursor ion experiments on scanning tandem MS instruments are of relatively low efficiency due to the requirement to scan the first mass analyser over a se-lected mass range. With tandem quadrupole instruments, the second quadru-pole operates at the fixed m/z of the product ion and so transmission efficiency is high, however, with Q-ToF type instruments MS2 is the ToF analyser which in-troduces further losses in ion transmission, in part due to low duty cycle, espe-cially at low m/z. The resulting duty cycle can be typically less than 0.1%. The oa-TOF duty cycle may be significantly increased by transporting ions to the orthogonal acceleration region using a travelling wave (T-Wave) ion guide1. The T-Wave ion guide converts the continuous ion beam into discrete packets and by subsequent synchronization of the arrival of ions of a specific m/z value from each packet with the ToF acceleration step, signal enhancement occurs over a m/z window centred on the ion of interest. Here we report a method of further improving the duty cycle on a Q-ToF instrument by replacing the scan-ning quadrupole with a T-Wave ion mobility separator1.

OVERVIEW

INTRODUCTION

EXPERIMENTAL

CONCLUSIONS

AIM - To enhance precursor ion sensitivity on a Q-ToF instrument METHOD - T-Wave ion mobility separator used to replace scanning quadrupole - ToF duty cycle increase using synchronization with T-Wave ion guide RESULTS - TOF duty cycle enhancement up to x10 - Combination with ion mobility separation of precursors provides up to x100 increase in response over precursor ion scanning on a tan-dem quadrupole

Lit Code Number 72000xxxxEN

The mobility separated ions can be injected into the T-Wave collision cell with low energy to allow recording of the precursor ions, or with high energy to record fragment ions. The continually running T-Wave in the cell maintains the mobility profile in both low and high energy cases and thus enables correlation of fragment ions with associated precursors. Enhancement of the ToF duty cycle (EDC) for a product ion is achieved by synchronising the ToF pusher with the ion packet release from the cell T-Wave and introducing a fixed delay to account for the time of flight of the ion of interest from the cell to the pusher region. Experimental Parameters Nano ESI interface - samples infused at 400 nL/min Quattro Premier Modified Q-ToF Premier T-Wave Collision Cell IMS Cell Collision Cell Pressure 4.0x10-3 mbar Ar 1.0 mbar N2 2.0x10-2 mbar N2 Pulse Height 5 V 21 V 3.5 V Pulse Velocity 300 m/s 600 m/s 300 m/s Acquisition Precursor ion scan IMS ToF MS 50-1100 Da in 1s 0-1950 Da Time 30 s 30 s

RESULTS Figure 2 shows the mass spectra obtained by conventional tandem quadru-pole precursor ion scanning for decreasing concentrations of verapamil in 70/30 acetonitrile/water (0.1% formic acid). From a comparison of rows 2 and 3 of Figure 2, it is reasonable to assume that the limit of detection (LOD) for this technique, defined by a continuous peak half-width, is approximately 1-2pg/µL. The ion mobility spectra and associated mass spectra of Figure 3 illustrate the technique of enhanced precursor ion scanning on a modified Q-ToF Premier MS. Row 1 shows the low collision energy ion mobility separation and the summed mass spectrum for 500fg/µL of verapamil.

Figure 1 Schematic diagram of the modified Q-Tof Premier instrument

• This study has demonstrated the feasibility of using a mobility-based sepa-

ration of precursor ions to enhance duty cycle when compared with a scan-ning quadrupole.

• Further increases in performance have been demonstrated by using EDC

acquisition on the oa-TOF analyser. • This technique has been shown to enhance the sensitivity of precursor ion

detection by at least a factor of 100 when compared with a scanning tan-dem quadrupole.

REFERENCES 1 ‘Applications of a Travelling Wave-Based Radio-Frequency-Only Stacked Ring Ion Guide’, Giles K, Pringle SD, Worthington KR, Little D, Wildgoose JL and Bateman RH, Rapid Commun. Mass Spectrom., 14 (2004) 2401

T-Wave Ion Mobility Separator The T-Wave ion guide is a stacked ring electrode device with opposite phases of RF applied to adjacent electrodes to provide radial ion confinement. A DC voltage pulse pattern is applied to the ring electrodes so as to provide travelling voltage pulses which propel ions through the gas-filled device. Through appro-priate choice of the wave parameters mobility separation can be achieved as less mobile ions roll over the ‘wave’ more often than the high mobility ions1. Experiments were performed on a modified Q-Tof Premier instrument, shown in Figure 1. Modifications include a separately pumped chamber which houses the T-Wave IMS cell, followed by a further pumped chamber containing a hex-apole ion guide and a T-Wave collision cell. To perform mobility-based separa-tions, ions are stored in the source T-Wave ion guide and gated out for 100 µs every 15 ms. The ion packet then enters the T-Wave IMS cell where mobility separation occurs and the ions then pass to the T-Wave collision cell through which they are transported to the ToF analyser. The temporal arrival of the mobility separated ions at the ToF is recorded by synchronisation of the orthogonal acceleration pushes (mass spectra) with the ion gate such that 200 mass spectra are recorded per mobility spectrum (total time per mobility spectrum = 200 x pusher period). The combination of ion accumulation during mobility separation and the high TOF repetition rate compared to mobility seaparation time scales significantly enhances duty cycle over that of a scanning quadrupole.

Figure 2 Conventional tandem quadrupole precursor ion scans for decreasing amounts of verapamil

Figure 3 Ion mobility separations and their associated mass spectra obtained under low and high energy conditions for 500fg/µL verapamil Figure 5 Conventional tandem quadrupole precursor ion scans for decreasing

amounts of beta casein digest

Figure 4 LOD for precursor ion scanning of 5fg/µL verapamil with the modified Q-Tof Premier

Figure 6 Ion mobility separations and their associated mass spectra obtained under low and high energy conditions for 100fmol/µL beta casein digest

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Row 2 of Figure 3 shows the selected ion mo-bility spectrum for the precursor ion of verapa-mil at m/z 455.34. By repeating this experi-ment at a high collision energy, we obtain the corresponding selected ion mobility spectrum for the product ion at m/z 165.15 and the associated mass spectrum obtained by selec-tively summing over the time-separated prod-uct ion mobility peak. The retention time and peak shape correlation between the two se-lected ion mobility spectra provides a high de-gree of precursor ion confirmation. Further-more, high sensitivity is demonstrated in the EDC product ion mass spectrum shown in row 4 (≈10x signal enhancement over the non-EDC acquisition mode). The LOD of verapamil at approximately 5fg/µL (Figure 4), represents an improvement factor of at least x200 when compared to the tandem quadrupole data. Figure 5 shows the mass spectra obtained by conventional tandem quadrupole precursor ion scanning for decreasing concentrations of a beta casein digest in 25/75 acetonitrile/water (0.1% formic acid).

At a total digest concentration of 100fmol/µL (row 3), we can estimate that the doubly charged precursor ion (FQBEEQQQTEDELQDK) contains around 10 ions! In contrast, the 100fmol/µL data for the modified Q-ToF Premier, shown in Figure 6, demonstrates a high degree of precursor ion confirmation (rows 2 and 4). Furthermore, the first two isotopes for the dephosphorylated product ion spectrum (Figure 6, row 4) contain approximately 1000 ion counts (EDC factor ≈4x). These data reinforce the assertion that the modified Q-ToF premier precur-sor ion scanning technique can show x100 improvements in sensitivity when compared to a tandem quadrupole instrument.

RP

TP

TP

TP

TP

TP

T-Wave(Source)

Quadrupole

Ion Gate

HexapoleIon Guide

T-Wave(IMS)

EinzelLens

oa-ToF

TransferLenses

ESISource

100 10-3 10-5 10-7(mbar)

T-WaveCollision

Cell

RP

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Quadrupole

Ion Gate

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T-Wave(IMS)

EinzelLens

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100100 10-310-3 10-510-5 10-710-7(mbar)

T-WaveCollision

Cell

Scan600 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800

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VER_EDC_10 TOF MS ES+ 455.26_455.42

112

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TOF MS ES+455.26_455.42

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VER_EDC_10 704 (1.239) TOF MS ES+ 130455.34

381.34

413.30

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TOF MS ES+130

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VER_EDC_10 704 (1.239) TOF MS ES+ 130455.34

381.34

413.30

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VER_EDC_10 704 (1.239) TOF MS ES+ 130455.34

381.34

413.30

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TOF MS ES+130

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VER_EDC_17 TOF MS ES+ TIC

2.62e3

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2.62e3

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2.62e3

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VER_EDC_17 865 (1.521) TOF MS ES+ 1.02e373.06

147.12

281.15

207.10

325.09

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TOF MS ES+1.02e3

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VER_EDC_17 865 (1.521) TOF MS ES+ 1.02e373.06

147.12

281.15

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325.09

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VER_EDC_17 865 (1.521) TOF MS ES+ 1.02e373.06

147.12

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TOF MS ES+1.02e3

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VER_EDC_16AFAMM TOF MS ES+ 165.152 0.05Da

104

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104

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Low EnergyMobility Spectrum

High EnergyMobility Spectrum

Precursor IonMobility Spectrumm/z 455.34

Product IonMobility Spectrumm/z 165.15

m/z50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950

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VER_EDC_16AFAMM 897 (1.577) TOF MS ES+ 7.16e3147.12

207.09

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TOF MS ES+7.16e3

m /z155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

156 158 160 162 164 166 168 170 172 174m/z

TOF MS ES+182

m /z155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

156 158 160 162 164 166 168 170 172 174m/z

TOF MS ES+182

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207.09

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207.09

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TOF MS ES+7.16e3

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

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TOF MS ES+182

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

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VER_EDC_10 645 (1.136) TOF MS ES+ 132455.34

239.20

172.23

202.21

301.19 381.35

345.24 413.33

663.54

701.50

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TOF MS ES+132

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VER_EDC_10 645 (1.136) TOF MS ES+ 132455.34

239.20

172.23

202.21

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345.24 413.33

663.54

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112

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112

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112

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VER_EDC_10 704 (1.239) TOF MS ES+ 130455.34

381.34

413.30

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VER_EDC_10 704 (1.239) TOF MS ES+ 130455.34

381.34

413.30

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VER_EDC_10 704 (1.239) TOF MS ES+ 130455.34

381.34

413.30

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2.62e3

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VER_EDC_17 865 (1.521) TOF MS ES+ 1.02e373.06

147.12

281.15

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VER_EDC_17 865 (1.521) TOF MS ES+ 1.02e373.06

147.12

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147.12

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207.09

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TOF MS ES+7.16e3

m /z155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

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TOF MS ES+182

m /z155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

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TOF MS ES+182

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207.09

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207.09

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TOF MS ES+7.16e3

m /z155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

156 158 160 162 164 166 168 170 172 174m/z

TOF MS ES+182

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V ER_EDC_16 897 (1.577) TOF MS E S+ 182165.15

166.16

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TOF MS ES+182

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VER_EDC_10 645 (1.136) TOF MS ES+ 132455.34

239.20

172.23

202.21

301.19 381.35

345.24 413.33

663.54

701.50

100 200 300 400 500 600 700 800 900 1000m/z

TOF MS ES+132

m/z50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000

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VER_EDC_10 645 (1.136) TOF MS ES+ 132455.34

239.20

172.23

202.21

301.19 381.35

345.24 413.33

663.54

701.50

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TOF MS ES+132

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Scan

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VER_EDC_11 890 (1.565) TOF MS ES+ 26.9

165.08

164.99162.13

163.10

167.12

167.02165.14

162 163 164 165 166 167 168m/z

TOF MS ES+26.9

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VER_EDC_11 890 (1.565) TOF MS ES+ 26.9

165.08

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167.12

167.02165.14

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VER_EDC_11 890 (1.565) TOF MS ES+ 26.9

165.08

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5fg/µL

Blank

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VER_EDC_12 896 (1.575) TOF MS ES+ 28

162.14

165.15

165.08

163.09

167.12

166.16

167.16

162 163 164 165 166 167 168m/z

TOF MS ES+28

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VER_EDC_12 896 (1.575) TOF MS ES+ 28

162.14

165.15

165.08

163.09

167.12

166.16

167.16

162 163 164 165 166 167 168m/z

TOF MS ES+28

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VER_EDC_12 TOF MS ES+ 165.109_165.19

46

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46

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VER_EDC_11 890 (1.565) TOF MS ES+ 26.9

165.08

164.99162.13

163.10

167.12

167.02165.14

162 163 164 165 166 167 168m/z

TOF MS ES+26.9

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VER_EDC_11 890 (1.565) TOF MS ES+ 26.9

165.08

164.99162.13

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167.12

167.02165.14

162 163 164 165 166 167 168m/zm/z

162 163 164 165 166 167 168

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VER_EDC_11 890 (1.565) TOF MS ES+ 26.9

165.08

164.99162.13

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167.12

167.02165.14

162 163 164 165 166 167 168m/z

TOF MS ES+26.9


5fg/µL

Blank

m/z162 163 164 165 166 167 168

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VER_EDC_12 896 (1.575) TOF MS ES+ 28

162.14

165.15

165.08

163.09

167.12

166.16

167.16

162 163 164 165 166 167 168m/z

TOF MS ES+28

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VER_EDC_12 896 (1.575) TOF MS ES+ 28

162.14

165.15

165.08

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TOF MS ES+28

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B_CAS_EDC_01 TOF MS ES+ TIC

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0 50 100 150 200ScanScan

800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.95e4

0 50 100 150 200Scan

TOF MS ES+2.95e4

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_01 TOF MS ES+ 1031.309_1031.43

146

0 50 100 150 200Scan

TOF MS ES+1031.309_1031.43

146

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_01 TOF MS ES+ 1031.309_1031.43

146

0 50 100 150 200ScanScan

800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_01 TOF MS ES+ 1031.309_1031.43

146

0 50 100 150 200Scan

TOF MS ES+1031.309_1031.43

146

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_01 922 (1.621) TOF MS ES+ 4191031.94

643.39

568.42

990.63

880.56

708.35

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_01 922 (1.621) TOF MS ES+ 4191031.94

643.39

568.42

990.63

880.56

708.35

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_01 922 (1.621) TOF MS ES+ 4191031.94

643.39

568.42

990.63

880.56

708.35

150 250 350 450 550 650 750 850 950 1050m/z

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_02 888 (1.561) TOF MS ES+ 1.80e3244.16

372.20

315.20

830.36

748.34594.31441.30488.28

660.34

983.44

1031.42

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.80e3

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


372.20

315.20

830.36

748.34594.31441.30488.28

660.34

983.44

1031.42

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


372.20

315.20

830.36

748.34594.31441.30488.28

660.34

983.44

1031.42

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.80e3

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_07 TOF MS ES+ 982.25_982.62

96.0

0 50 100 150 200Scan

TOF MS ES+982.25_982.62

96.0

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_07 TOF MS ES+ 982.25_982.62

96.0

0 50 100 150 200ScanScan

800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_07 TOF MS ES+ 982.25_982.62

96.0

0 50 100 150 200Scan

TOF MS ES+982.25_982.62

96.0

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/zm/z

972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+97.7

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/zm/z

972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7





m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


646.40

210.01

355.10

284.19

568.41502.39443.10

693.39

780.50

830.46

991.13

880.56

1032.48

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.31e3

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


646.40

210.01

355.10

284.19

568.41502.39443.10

693.39

780.50

830.46

991.13

880.56

1032.48

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.31e3

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.73e4

0 50 100 150 200

TOF MS ES+TIC

2.73e4

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.73e4

0 50 100 150 200

TOF MS ES+TIC

2.73e4

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.95e4

0 50 100 150 200Scan

TOF MS ES+2.95e4

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.95e4

0 50 100 150 200ScanScan

800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.95e4

0 50 100 150 200Scan

TOF MS ES+2.95e4

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_01 TOF MS ES+ 1031.309_1031.43

146

0 50 100 150 200Scan

TOF MS ES+1031.309_1031.43

146

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_01 TOF MS ES+ 1031.309_1031.43

146

0 50 100 150 200ScanScan

800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_01 TOF MS ES+ 1031.309_1031.43

146

0 50 100 150 200Scan

TOF MS ES+1031.309_1031.43

146

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_01 922 (1.621) TOF MS ES+ 4191031.94

643.39

568.42

990.63

880.56

708.35

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_01 922 (1.621) TOF MS ES+ 4191031.94

643.39

568.42

990.63

880.56

708.35

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_01 922 (1.621) TOF MS ES+ 4191031.94

643.39

568.42

990.63

880.56

708.35

150 250 350 450 550 650 750 850 950 1050m/z

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


372.20

315.20

830.36

748.34594.31441.30488.28

660.34

983.44

1031.42

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.80e3

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


372.20

315.20

830.36

748.34594.31441.30488.28

660.34

983.44

1031.42

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


372.20

315.20

830.36

748.34594.31441.30488.28

660.34

983.44

1031.42

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.80e3

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_07 TOF MS ES+ 982.25_982.62

96.0

0 50 100 150 200Scan

TOF MS ES+982.25_982.62

96.0

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_07 TOF MS ES+ 982.25_982.62

96.0

0 50 100 150 200ScanScan

800 820 840 860 880 900 920 940 960 980 1000

%

0

100

B_CAS_EDC_07 TOF MS ES+ 982.25_982.62

96.0

0 50 100 150 200Scan

TOF MS ES+982.25_982.62

96.0

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/zm/z

972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+97.7

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/zm/z

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

876.41714.92

660.37

747.39

859.40

845.40

748.38

974.42

973.93

933.55

1042.90

1043.39

1050.88

1063.36

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7

m/z972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/zm/z

972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992

%

0

100

B_CAS_EDC_07 930 (1.635) TOF MS ES+ 97.7982.43

977.45

974.42973.93

973.42

978.46

979.44

983.42

983.93

987.43984.92 990.58

973 975 977 979 981 983 985 987 989 991m/z

TOF MS ES+97.7





m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


646.40

210.01

355.10

284.19

568.41502.39443.10

693.39

780.50

830.46

991.13

880.56

1032.48

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.31e3

m/z150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050

%

0

100


646.40

210.01

355.10

284.19

568.41502.39443.10

693.39

780.50

830.46

991.13

880.56

1032.48

150 250 350 450 550 650 750 850 950 1050m/z

TOF MS ES+1.31e3

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.73e4

0 50 100 150 200

TOF MS ES+TIC

2.73e4

Scan800 820 840 860 880 900 920 940 960 980 1000

%

0

100


2.73e4

0 50 100 150 200

TOF MS ES+TIC

2.73e4

165.15

166.16

Documents

ENHANCEMENT OF PRECURSOR ION SCANNING ON A Q-TOF … · 2007. 11. 21. · Steve Bajic, Robert H Bateman, Kevin Giles, Steven D Pringle and Jason L Wildgoose Waters Corporation, MS