Electronic Supplementary Material

Patterned gold electrode prepared from optical discs display largely enhanced

electrochemical sensitivity as exemplified in a sensor for hydrogen peroxide

Aroonsri Ngamaroonchote, Chuleekorn Chotsuwan, Kittipong Tantisantisom, Rawiwan

Laocharoensuk*

National Nanotechnology Center (NANOTEC), National Science and Technology Development

Agency (NSTDA), Pathum Thani 12120, Thailand

*Corresponding author: rawiwan.lao@nanotec.or.th

Fig. S1 Cyclic voltammograms of gold electrodes fabricated from flat polycarbonate, BD-R and

BD-ROM in 0.5 M H2SO4 with a scan rate 50 mVs-1

The electrochemical surface area (ECSA) of gold electrodes prepared from gold flat PC,

gold BD-R and gold BD-ROM was determined as shown in Fig. S1. The CV profiles exhibit an

anodic peak at 1.38 V vs. Ag/AgCl and cathodic peak at 0.91 V vs. Ag/AgCl, which correspond

to the oxidation of gold and the reduction of gold oxide, respectively. The charge obtained by

integration of the gold oxide reduction peak was used for the calculation of ECSA values [1]. A

value of 390±10 µCcm-2 has been suggested for polycrystalline Au [2]. The ECSA values for

gold BD-ROM, gold BD-R and gold flat electrode are 0.258, 0.142 and 0.133 cm2, respectively.

Fig. S2 FE-SEM images of gold film coated on (a) flat polycarbonate, (b) CD-R, (c) CD-ROM,

(d) DVD-R, (e) DVD-ROM, (f) BD-R and (g) BD-ROM

The FE-SEM images of all gold electrodes were shown in Fig. S2. As can be seen,

electrode surface revealed stripe and indented patterns depending on PC substrate used for

electrode fabrication. All samples presented polycrystalline structure of Au film prepared from

sputtering technique [3].

Table S1 a comparison of sensing performance of enzyme-based and enzyme-free H2O2 sensors

Sensing material/electrode LOD (M) Sensitivity

(µA·mM-1cm-2)

Linear range

(mM)

Ref

Enzyme-based sensors

HRP-AuNPs-sol-gel/Au electrode

2 10-6 NA 5 10-3-1 10-2 [4]

HRP-AuNPs/ITO electrode 2 10-6 NA 8 10-6-3 10-3 [5]

HRP-nanoAu/CCE 6.1 10-6 NA 1.2 10-5 -1.1 10-3 [6]

HRP-SGCCN/GCE 1.3 10-7 NA 1.3 10-5–1.1 10-2 [7]

Au-SPAN-HRP-CS/GCE 1.6 10-6 NA 1 10-5-2 10-3 [8]

Enzyme-free sensor

Roughened Ag 6 10-6 NA 1 10-5 – 2.2 10-2 [9]

AgNWs array 2.9 10-5 26.6 1 10-4 – 3.1 10-3 [10]

Macroporous Au 5 10-5 110 5 10-4-1 10-2 [11]

Macroporous Au-PtNPs 5 10-5 264 5 10-4-1 10-2 [11]

Pt nanoflowers 6 10-5 NA 1 10-4–9 10-4 [12]

PQ11-AgNPs 3.4 10-5 NA 1 10-4– 1.8 10-1 [13]

Mesoporous Pt microelectrodes 4.5 10-6 NA 2 10-5–4 10-2 [14]

Graphene-AuNPs/GCE 6 10-6 3 2 10-5–2.8 10-4 [15]

AgNPs-Graphene/ITO electrode 5 10-6 NA 1 10-4-1 10-1 [16]

PdCu/SPCE 7 10-7 396.7 5 10-4–1.1 10-2 [17]

Patterned Au-FE 6 10-6 3.11 2.5 10-3-10 10-3 This work

Abbreviation: horseradish peroxidase (HRP), nanoparticles (NPs), indium tin oxide (ITO),

carbon ceramic electrode (CCE), sol–gel-derived ceramic–carbon nanotube (SGCCN), glassy

carbon electrode (GCE), self-doped polyaniline nanofibers (SPAN), chitosan (CS), poly[(2-

ethyldimethylammonioethyl methacrylate ethyl sulfate)-co-(1-vinylpyrrolidone)] (PQ11), screen-

printed carbon electrode (SPCE)

Fig. S3 The current response of the gold BD-ROM electrode for H2O2 reduction at −0.1 V vs.

Ag/AgCl in presence of interferences.

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