Electives JRF

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    CONTENTS

    1. Animal Biotechnology .............................................................. 12. Animal Cytogenetics and Immunogenetics ............................ 23. Animal Population Genetics ..................................................... 34. Bacteriology ............................................................................... 35. Bioentrepreneurship .................................................................. 46. Biogenerics & Biopharmaceuticals........................................... 5

    7. Bioinformatics ............................................................................ 68. Biometrical Techniques in Animal Breeding ........................... 79. Bioprocess Engineering and Technology................................. 8

    10. Bioreaction Engineering ........................................................... 911. Biostatistics ................................................................................. 1012. Cancer Genetics ......................................................................... 1113. Cell & Developmental Biology ................................................. 1114. Clinical Genetics & Counseling ................................................ 1215. Clinical Trials & Bioethics ......................................................... 1416. Computational Biology ............................................................. 1417. Diagnostics ................................................................................. 1518. Environmental Biotechnology .................................................. 1619. Environmental Engineering ..................................................... 1720. Evolutionary Genetics ............................................................... 1921. Female Infertility ....................................................................... 19

    22. Food Process & Biotechnology ................................................. 2023. General Andrology .................................................................... 2124. General Gynaecology ............................................................... 2225. General Virology ....................................................................... 2226. Genetics ...................................................................................... 2327. Genoinformatics ........................................................................ 2528. Genomics & Proteomics ............................................................ 2629. Industrial & Food Biotechnology ............................................. 26

    30. Male Infertility ........................................................................... 27

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    31. Marine Food Technology .......................................................... 2832. Metabolic Engineering .............................................................. 28

    33. Microbial Technology ............................................................... 2934. Model Genetic Systems ............................................................. 3035. Molecular Techniques in Animal Breeding .............................. 3036. Molecular Therapeutics ............................................................. 3137. Molecular Virology ................................................................... 3238. Nanobiotechnology ................................................................... 3239. Nanobiotechnology (specific to Neurosciences)........................ 3340. Neurogenetics ............................................................................ 33

    41. Neuroinformatics ...................................................................... 3442. Ornamental Fish Culture & Aquarium Keeping .................... 3543. Pharmaceutical Biotechnology ................................................ 3544. Pharmacogenomics ................................................................... 3645. Plant Biotechnology .................................................................. 3746. Plant Metabolite Engineering .................................................. 3847. Process Control & Instrumentation .......................................... 3948. Protein Engineering .................................................................. 40

    49. Reproductive Genetics ............................................................... 4050. Stem Cell Biology ...................................................................... 4151. Systematic Animal Virology ..................................................... 4252. Vaccines ...................................................................................... 42

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    List of Electives

    1. Animal Biotechnology - 3 Credits

    Unit I Animal cell cultureStructure of animal cell; History of animal cell culture; Basic requirements for animal cell culture; Cellculture media and reagents; Animal cell, tissue and organ cultures; Primary culture, secondary culture;Continuous cell lines; Suspension cultures; Somatic cell cloning and hybridization; Transfection andtransformation of cells; Commercial scale production of animal cells; Stem cells and their application;

    Application of animal cell culture for in vitro testing of drugs; Testing of toxicity of environmentalpollutants in cell culture; Application of cell culture technology in production of human and animal vaccinesand pharmaceutical proteins.

    Unit II Animal health BiotechnologyIntroduction to immune system; Cellular and humoral immune response; History of developmentof vaccines; Introduction to the concept of vaccines; Conventional methods of vaccine production;Recombinant approaches to vaccine production; Hybridoma technology; Phage display technology forproduction of antibodies; Antigen-antibody based diagnostic assays including radioimmunoassaysand enzyme immunoassays; Immunoblotting; Nucleic acid based diagnostic methods includingnucleic acid probe hybridization; Restriction endonuclease analysis; PCR, Real time PCR; Nucleicacid sequencing; Commercial scale production of diagnostic antigens and antisera; Animal disease diagnostickits; Probiotics.

    Unit III Animal Reproductive BiotechnologyStructure of sperms and ovum; Cryopreservation of sperms and ova of livestock; Artificial insemination;Super ovulation; in vitro fertilization; Culture of embryos; Cryopreservation of embryos; Embryo transfer;Embryo-splitting; Embryo sexing; Micromanipulation of animal embryos; Transgenic animal technologyand its different applications; Animal viral vectors; Animal cloning- basic concepts; Cloning from embryoniccells and adult cells; Cloning of farm animals; Cloning for conservation of endangered species; Ethical, socialand moral issues related to cloning; in situ and ex situ preservation of germplasm; in utero testing of foetus forgenetic defects; Pregnancy diagnostic kits; Anti-fertility animal vaccines.

    Unit IV Animal genomicsIntroduction to different breeds of cattle, buffalo, sheep, goats, pigs, camels, horses, canines and poultry;Genetic characterization of livestock breeds; Marker assisted breeding of livestock and poultry; Introductionto animal genomics; Different methods for characterization of animal genomes, SNP, STR, QTLS, RFLP,RAPD, proteomics, metobolomics; Genetic basis for disease resistance; Gene knock out technology and

    animal models for human genetic disorders.

    LIST OF ELECTIVES

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    List of Electives

    Unit VDNA ForensicsImmunological and nucleic acid based methods for identification of animal species; Detection of adulterationin meat using DNA based methods; Detection of food/feed adulteration with animal protein; Identification ofwild animal species using DNA based methods using different parts including bones, hair, blood, skin andother parts confiscated by anti-poaching agencies; Human forensics; Microbial forensics; Bioterror agents;Biocrimes and Bioterrorism.

    Texts/References1. Ed. John R.W. Masters, Animal Cell Culture - Practical Approach, 3 rd Edition, Oxford University Press, 2000.2. Ed. Martin, Clynes Animal Cell Culture Techniques, Springer, 1998.3. Animal Cell Biotechnology. Portner, 2nd Edition, Humana Press, 2007.4. A. Puller (ed), Genetic engineering in Animals, VCH Publishers.

    5. Gordon, Reproductive Technologies in Farm Animals, CAB Intl., 2005.6. Pinkert, Transgenic animal technology, Academic Press, 2006.

    2.Animal Cytogenetics & Immunogenetics - 3 CreditsUnit IDevelopment in animal cytogenetics and immunogenetics of farm animals; Immunoglobulins and theirtypes; Antigen-antibody interactions.

    Unit IIMajor histocompatibility complex; Genetics of biochemical variants and their application; Ir-genes and conceptsof disease resistance including major genes; Hybridoma and its significance; Concept of immuno-fertility.

    Unit IIIChromatin structure of eukaryotes; Chromosome number and morphology in farm animals banding andkaryotyping; Chromosomal and genetic syndromes.

    Unit IVMutation and assays of mutagenesis; Sister chromatid exchanges; Recombinant DNA technique and itsapplication in animal improvement programme.

    PracticalsPolymorphism of haemoglobulins, transferrins, enzymes/proteins; Preparation of monovalent blood reagent-isoimmunization, Titre testing and absorption of polyvalent serum; Identification of bar bodies; In vitro andin vivo preparation of somatic metaphase chromosomes; Screening of chromosomal abnormalities;Microphotography and karyotyping; Banding