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Elecsys® Assay interferences
Page 1 Version 4.6 Sep. 2014
The assay is unaffected by Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)#
Rheumatoid factors (U/ml)#
Dialysis
Others
Thyroid FT3 III 06437206
< 1128 < 0.621 < 2000 < 286 < 1200 IgG < 7 g/dl IgM < 1 g/dl
IgA < 1.6 g/dl FT4 II
06437281 < 701 < 0-621 < 2000 < 81.8 < 339 IgG < 7 g/dl
IgM < 1 g/dl IgA < 1.6 g/dl
T3 11731360
< 599 < 1.2 < 1800 < 40.9 > 40.9 ↑
< 1500 no interference
T4 12017709
< 633 < 1.4 < 2500 < 409 < 2400 no interference
TSH 11731459
< 701 < 0.621 > 0.621 ↓
< 1800 < 102 > 102 ↓
< 3250 no interference
T-uptake 11731394
< 701 < 1.2 < 2000 < 164 > 164 ↓
< 339 no interference
Calcitonin 06445853
< 1128 < 0.124 < 2000 < 163 < 1200 IgG < 4 g/dl IgM < 0.7 g/dl IgA < 1.6 g/dl
Tg II 05118921
< 1128 < 0.373 < 2000 < 123 < 600 IgG ≤ 2 g/dl IgM ≤ 0.5 g/dl IgA ≤ 1.6 g/dl
Anti-Tg 06368697
< 1129 < 1.05 < 2000 < 246 < 300
Anti-TPO 06368590
< 1129 < 0.93 < 2000 < 40.9 < 1500
Anti-TSHR
04388780
< 427 < 0.248 < 1500 < 41 < 600
Elecsys® Assay interferences
Page 2 Version 4.6 Sep. 2014
The assay is unaffected by
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB
mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Others
Cardiac CK-MB STAT 05957648
CK-MB 05894808
< 581 < 0.311 < 1500 < 123 < 1500 no interference
IgG < 7 g/dl IgM < 1 g/dl IgA < 6 g/dl
Albumin < 20 g/dl Digoxin
11820796 < 1112 < 0.621 < 1500 < 409 < 1630
Digitoxin 03002659
< 633 < 0.683 > 0.683 ↑
< 1500 < 205 < 1500
Myoglobin STAT 11820788 Myoglobin 12178214
< 1112 < 0.869 < 2200 < 205 < 1500
proBNP II STAT 05390109 proBNP II 04842464
< 428 < 0.621 < 1500 < 123 < 1500
Troponin I STAT 04660307 Troponin I 05094810
< 428 < 0.247 > 0.247 ↓
< 1500 < 123 < 1500
Troponin T hs STAT
05092728 Troponin T hs
05092744
< 428 < 0.062 > 0.062 ↓
< 1500 < 82 > 82 ↓
< 1500
Elecsys® Assay interferences
Page 3 Version 4.6 Sep. 2014
The assay is unaffected by
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB
mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Others
Hormones ACTH 03255751
< 428
< 0.25 > 0.25 ↓
< 1500 < 246 < 400 > 400 ↑
AMH 06331076
< 1129 < 0.621 < 1000
< 143
< 1000 IgG ≤ 2.5 g/dl IgM ≤ 0.5 g/dl IgA ≤ 1.8 g/dl
Cortisol 11875116
< 1026 < 1.2 < 2700 < 123 < 1100
DHEA-S 03000087
< 222 > 222 ↑
< 0.35 > 0.35 ↑
< 2000 < 123 > 123 ↑
< 600 > 600 ↑
Estradiol II 03000079
< 1129 < 0.621 < 1000 > 1000 ↓
< 147 > 147 ↑
< 1200
FSH 11775863
< 1094 < 0.621 < 1900 < 246 > 246 ↓
< 2250
HCG STAT 03300811
< 496 > 496 ↓
< 0.932 < 2400 < 164 < 667 > 667 ↑
no interference
no interference
HCG+b 03271749
< 410
< 0.621 < 1400 < 327 < 3400
no interference
no interference
LH 11732234
< 1129 < 0.621 < 1900 < 205 > 205 ↓
< 1500
Progesterone II
12145383
< 923 > 0.923 ↑
< 0.621 < 720 > 720 ↓
< 82 > 82 ↑
< 2000
Elecsys® Assay interferences
Page 4 Version 4.6 Sep. 2014
The assay is unaffected by
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB
mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Hormones Prolactin II 03203093
< 513 < 0.932 < 1500 > 1500 ↓
< 164 < 1100
SHBG 03052001
< 1026 < 1.8 < 2700 < 246 < 1160
Testosterone II 05200067
< 513 < 0.372 < 1000 > 1000 ↓
< 123
< 1000
Diabetes C-Peptide 03184897
< 855 < 0.186 > 0.186 ↓
< 2000 < 246 < 1200
Insulin 12017547
< 1539 Hemolysis interferes, as insulin-degrading peptidases
are released
from erythrocytes
< 1800 < 246 < 18900 > 18900 ↑
Pree-clampsia
PlGF 05144671
< 428 < 0.311 < 1500
< 123 < 600
sFlt-1 05109523
< 427 < 0.311 < 1400 < 123 > 123 ↓
< 600
Elecsys® Assay interferences
Page 5 Version 4.6 Sep. 2014
The assay is unaffected by
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB
mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Tumor Marker
AFP 04481798
< 1112 < 1.4 < 1500 < 246 < 1500
CA 125 II 11776223
< 1129 < 2.0 < 2000 < 143 > 143 ↓
< 1200
CA 15-3 II 03045838
< 1112 < 1.9 < 1500 < 409 < 1500
CA 19-9 11776193
< 1129 < 1.4 < 1500 < 409 > 409 ↓
< 1500
CA 72-4 11776258
< 1129 < 1.4 < 1500 < 246 > 246 ↓
< 1500
CEA 11731629
< 1129 < 1.4 < 1500 < 491 < 1500
Cyfra 21-1 11820966
< 1112 < 0.93 < 1500 < 205 < 1500
HE4 05950929
< 1130 < 0.621 < 2000 < 205 < 1500
NSE 12133113
< 1231 NSE in erythro-
cytes and platelets leads to elevated results in
hemolyzed samples
< 2000 < 409 < 1500
Elecsys® Assay interferences
Page 6 Version 4.6 Sep. 2014
The assay is unaffected by
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB
mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Others
Tumor Marker
ProGRP 06505961
≤ 1130 < 0.621 ≤ 2000 ≤ 35 < 540 IgG ≤ 17 g/l IgM < 2.9 g/l IgA < 10 g/l Albumin <
200 g/l free PSA
03289788 < 1112 < 0.621 < 1500 < 123 < 1500
total PSA 04641655
< 1112 < 1.4 < 1500 < 246 < 1500
S 100 03175243
< 428 > 428 ↑
< 0.621 > 0.621 ↓
< 1500 > 1500 ↑
< 205 > 205 ↓
< 1000
Down’s Syndrome
free ßHCG 0484071
< 428 > 428 ↓
< 0.621 < 1500 < 123 < 1000
Screening PAPP-A 04854098
< 205 > 205 ↓
< 0.621 > 0.621 ↓
< 1500 < 123 > 123 ↓
< 1000
Elecsys® Assay interferences
Page 7 Version 4.6 Sep. 2014
The assay is unaffected by
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Anaemia Vitamin B12 04745736
< 1112 < 0.621 < 1500 < 205 < 1500
Ferritin 03737551 04491785
< 1112 < 0.31 > 0.31 ↑
< 3300 < 205 < 2500
Folate III 04476433
< 564 > 564 ↑
Hemolysis may significantly
increase folate values due to high concentrations of folate in red blood cells. Therefore
hemolyzed samples are not suitable for use in this assay.
< 1500 < 21 > 21 ↓
< 1000
Folate RBC 05944295
< 564
- < 1500 < 21 < 1000
Elecsys® Assay interferences
Page 8 Version 4.6 Sep. 2014
The assay is unaffected by Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)#
Rheumatoid factors (U/ml)#
Dialysis
Others
Bone b-CrossLaps/ serum
11972308
< 1112 < 0.3 > 0.3 ↓
< 1500 < 368 > 368 ↓
< 1500
hGH 05390125
< 428 < 0.310 < 1500 < 123 < 600
N-MID Osteocalcin 12149133
< 1112 Erythrocytes contain proteases which
degrade osteocalcin
< 1500 < 205 < 2200
Total P1NP 03141071
< 1112 < 1.1 < 2000 < 205 > 205 ↓
< 2490
PTH 11972103
< 1112 < 0.09* > 0.09 ↓
< 1500 < 205 > 205 ↓
< 1500
PTH STAT 04892470
< 1112 < 0.155 < 1500 < 205 < 1500
PTH (1-84) 05608546
< 1112 < 0.062* < 1500 < 205 < 1500 IgG < 28 g/l IgM < 8 g/l IgA < 16 g/l
Albumin < 120 g/l
Vitamin D total 05894913
< 1129 < 0.124* > 0.124 ↑
< 400 < 287
-
Varia IgE II 04827031
< 633 < 0.06 > 0.06 ↓
< 2700 < 409 < 6000
* Samples showing visible signs of hemolysis may cause interference.
Elecsys® Assay interferences
Page 9 Version 4.6 Sep. 2014
The assay is unaffected by Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)#
Rheumatoid factors (U/ml)#
Dialysis
Others
Rheuma-toid Arthritis
Anti-CCP 05031656
< 427 > 427 ↓
< 0.311 < 1500 < 123 < 150
Sepsis BRAHMS PCT 05056888
< 428 < 0.559 < 1500 < 123 > 123 ↓
< 1500
IL-6 05109442
< 428 < 0.621 > 0.621 ↓
< 1500 < 123 > 123 ↓
< 1500
ISDs Cyclosporine 05889014
≤ 1026 - ≤ 1500 ≤ 123 < 500 IgG ≤ 12 g/l Albumin ≤ 12 g/l
Cholesterol ≤ 500 mg/dL
HAMA ≤ 50 µg/mL Hematokrit 15-60%
Uric acid ≤ 20 mg/dL Tacrolimus
05889057 ≤ 1026 - ≤ 1500 ≤ 123 < 500 IgG ≤ 12 g/l
Albumin ≤ 12 g/l Cholesterol ≤ 500
mg/dL HASA ≤ 10 µg/mL Hematokrit 15-60%
Uric acid ≤ 20 mg/dL
Elecsys® Assay interferences
Page 10 Version 4.6 Sep. 2014
The assay is unaffected by Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Hepatits Anti-HAV 11820605
< 855 < 0.745 > 0.745 ↓
< 1000 > 1000 ↓
< 205 < 1600
Anti-HAV IgM 11820591
< 855 < 1.09 < 2000 < 205 < 3200
Anti-HBc 11820559
< 428 < 1.0 > 1.0 ↓
< 1000 < 123 > 123 ↓
< 676 > 676 ↓
Anti-HBc IgM 11820567
< 428 < 1.2 < 1500 < 409 < 4200
Anti-HBe 11820613
< 85.5 > 85.5 ↓
< 1.2 < 1500 < 409 < 2400
HBeAg 11820583
< 428 < 0.99 > 0.99 ↑
< 1500 < 164 > 164 ↑
< 2400
Anti-HBs 11820524
< 513 > 513 ↓
< 0.93 < 1500 < 123 > 123 ↓
< 2100 > 2100 ↓
HBsAg II 04687787
< 684 < 1.24 > 1.24 ↓
< 2000 < 164 > 164 ↓
< 6210
HBsAg II quant 05957435
< 684 < 0.311 < 2000 < 164
< 1200
Anti-HCV II 06368921
< 1129 < 0.621 < 2000 < 172 < 1200
Elecsys® Assay interferences
Page 11 Version 4.6 Sep. 2014
Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Others
Sexually transmitted diseases (STD)
HIV antigen 11971611
< 428 < 1.2 < 1500 < 409 < 2400
HIV combi PT
05390095
< 1026 < 0.31 < 1500 < 123 < 1500
Syphilis 06923348
≤ 1129 ≤ 0.310 ≤ 2000 ≤ 246 < 1500 Human serum albumin ≤ 100 g / dl
Human IgG ≤ 32 g/l Human IgM ≤ 10 g/l Human IgA ≤ 2 g /dl
Elecsys® Assay interferences
Page 12 Version 4.6 Sep. 2014
The assay is unaffected by Reagent type
Assay cat.no.
Bilirubin (µmol/l)#
Hemolysis (HB mmol/l)#
Lipemia (Intralipid or triglycerides mg/dl)# Ë
Biotin (nmol/l)
#
Rheumatoid factors (U/ml)#
Dialysis
Torch CMV IgG 04784596
<1129 < 0.31 < 2000 < 246 < 1500
CMV IgG Avidity
05909708
< 1129 < 0.31 < 2000 < 246 < 1500
CMV IgM 04784618
< 342 < 0.31 < 1500 < 410 < 2000
HSV-1 IgG 05572185
<1130 <0.621 <2000 <205 <1500
HSV-2 IgG 05572193
<1130 <0.621 <2000 <205 <1500
Rubella IgG 04618793
< 513 < 3.47 < 1500 < 205 < 6210
Rubella IgM 04618831
< 428 < 1.49 < 1500 < 205 < 6210
Toxo IgG 04618815
< 684 < 1.24 < 2000 < 246 < 6210
Toxo IgG Avidity
05802571
< 684 < 1.24 < 2000 < 246 < 6210
Toxo IgM 04618858
< 684 < 1.24 < 2000 < 246 < 3720
Elecsys® Assay interferences
Page 13 Version 4.6 Sep. 2014
# Criterion: recovery within +/- 10% of initial value, when no other information is stated in the corresponding method sheet. Ë Please refer to method sheet whether Intralipid or tryglycerides have been used for interference testing of the Assay.
Elecsys® Assay interferences
Page 14 Version 4.6 Sep. 2014
Used Documents: Assay Method Sheet FT3 III 1212/01 FT4 II 1212/01 T3 0811/20 T4 1011/16 TSH 0911/20 T-uptake 1111/18 Calcitonin 0213/01 Tg II 0313/01 Anti-Tg 0911/02 Anti-TPO 0811/02 Anti-TSHR 0911/09 CK-MB 0812/03 Myoglobin 0812/14 proBNP II 0512/07 Troponin I 0712/05 Troponin T hs STAT 0512/07 Digoxin 0512/18 Digitoxin 0612/11 HCG STAT 0412/15 HCG+b 0412/15 ACTH 1211/07 AMH 0714/01 Cortisol 0512/08 DHEA-S 0912/16 Estradiol II 0312/02 FSH 0412/18 LH 0412/18 Progesterone II 0712/15
Elecsys® Assay interferences
Page 15 Version 4.6 Sep. 2014
Used Documents: Assay Package insert Prolactin II 0312/05 SHBG 0512/10 Testosterone II 0512/06 C-Peptide 0412/08 Insulin 0412/13 PlGF 0512/05 sFlt-1 0512/05 AFP 0911/10 CA 125 II 0412/15 CA 15-3 II 0512/16 CA 19-9 0911/20 CA 72-4 0312/10 CEA 0512/20 Cyfra 21-1 0812/15 HE4 0612/05 NSE 0812/11 ProGRP 0913/01 free PSA 0211/10 total PSA 0510/07 S100 0510/07 Free ßhCG 0812/06 PAPP-A 0411/03 Vitamin B12 0112/10 Ferritin 0312/12 Folate III 0712/10 Folate RBC 1011/01 IgE II 0312/06 b-CrossLaps/serum 1211/12 hGH 0211/02
Elecsys® Assay interferences
Page 16 Version 4.6 Sep. 2014
Used Documents: Assay Package insert N-MID Osteocalcin 1211/14 PTH 1012/22 PTH STAT 1211/01 PTH (1-84) 1012/02 Total P1NP 0812/09 Vitamin D total 0812/04 Anti-CCP 0611/04 BRAHMS PCT 0712/07 IL-6 0512/05 Cyclosporine 0913/01 Tacrolimus 0913/02 Anti-HAV 0712/09 Anti-HAV IgM 1012/12 Anti-HBc 0610/20 Anti-HBc IgM 0212/14 Anti-HBe 0610/13 HBeAg 0610/15 Anti-HBs 0610/16 HBsAg II 1112/11 HBsAg II quant 0111/02 Anti-HCV II 1112/02 HIV Ag 0712/11 HIV combi PT 1212/03 Syphilis 0114/01 CMV IgG 1012/06 CMV IgG Avidity 0712/03 CMV IgM 0712/05 HSV-1 IgG 1211/02
Elecsys® Assay interferences
Page 18 Version 4.6 Sep. 2014
Assay Package insert Rubella IgG 1012/06 Rubella IgM 1012/07 Toxo IgG 0612/10 Toxo IgG Avidity 0712/03 Toxo IgM 1211/07 For this document are the contents of the current ElecsysÒ method sheets used. The used method sheet versions are listed above. Original datas are available in the corresponding method sheets.