Eks Bab4 Kepekatan Enzim

8/11/2019 Eks Bab4 Kepekatan Enzim

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Berdasarkan maklumat di bawah, rancang satu eksperimen untuk mengkaji

kesan kepekatan enzim amilase air liur terhadap kadar tindakbalas

biokimia.

Terdapat banyak faktor yang mempengaruhi kebolehan enzim dalammemangkinkan sesuatu tindakbalas biokimia. Antara faktor-faktor tersebut ialah

pH, suhu, kepekatan substrat dan kepekatan enzim. Apabila kepekatan enzim

bertambah, maka terdapat lebih banyak molekul enzim.

Berdasarkan maklumat di atas, rancang satu eksperimen dalam makmal untuk

mengkaji kesan kepekatan enzim amilase air liur terhadap kadar tindakbalas

biokimia.

Perancangan kerja eksperimen anda perlu meliputi aspek-aspek berikut

1. Pernyataan masalah

2. Objektif kajian

3. Hipotesis

4. Pembolehubah5. Senarai alat radas dan bahan

6. Teknik yang digunakan

7. Kaedah atau prosedur eksperimen8. Cara data dikomunikasikan

9. Kesimpulan [17 marks]

CADANGAN JAWAPAN :

AI! "o stud#$ in%esti&ate t'e e((e)t o( sali%ar# am#lase $ en*#me )on)entration on t'e rate

o( +,io)'emi)al- rea)tion

/0 "A""!

'at is t'e e((e)t o( sali%ar# am#lase $ en*#me )on)entration on t'e rate o( +,io)'emi)al-

rea)tion

0"I

"'e 'i&'er t'e sali%ar# am#lase $ en*#me )on)entration: t'e 'i&'er t'e rate o( +,io)'emi)al-

rea)tion until it rea)'es a ma;imum rate

i;ed! p: su,strate )on)entration: temperature


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AA/A"? A@ A"/IA

eakers: test tu,es: s#rin&e: dropper: &lass rod: ='ite tile: t'ermometer: stop=at)': =ater

,at' +=ire &au*e: ,unsen ,urner: tripod stand-: test tu,e ra)k: star)' solution: iodine solution:

sali%ar# am#lase$ sali%a: distilled =ater

"CI?!

easure and recordt'e time taken (or ,reakdo=n $ '#rol#sis o( star)'$$ iodine solution to

)'an&e )olour (rom ,lue ,la)k to ,ro=nis' #ello= using a stopwatch

/0C@?/!

Able to list down the complete and correct procedure used based on the following criteriaK1 Technique of assembling the apparatus to carry

out the experiment.

K2 Technique of fixing the constant variables

K Technique of changing the manipulated

variables

K! Technique of measuring the responding

variables

K" # Technique of ta$ing precautions to increase accuracy

Suggested Answer:

1. a,el si; test tu,es as A: : C: @: and >. +K3-

2. >ill ea)' test tu,e =it' solutions as s'o=n in t'e ta,le ,elo=. +K1: K3-

"est tu,e


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7.Carr# out t'e iodine test at an inter%al o( 3B se)onds until t'e ,lue,la)k )olour no lon&er

appears and t'e iodine solution remains ,ro=nis' #ello=. +K1: K2-

8. /e)ord t'e time taken (or t'e )omplete '#drol#sis o( star)'. +K4-

9. I( ,lue ,la)k )olour is still produ)ed a(ter ei&'t minutes: terminate t'e e;periment =it' t'e

rea)tion mi;ture. +K5-1B. /epeat steps 4 to 8 (or test tu,es :C: @: and >. +K1: K3-

11. /e)ord all #our o,ser%ations. +K4-

12.lot a &rap' o( rate o( rea)tion [1$time] a&ainst en*#me )on)entration. +K1-

/?"!

C0C?I0!

"'e 'i&'er t'e sali%ar# am#lase $ en*#me )on)entration: t'e 'i&'er t'e rate o( +,io)'emi)al-rea)tion until it rea)'es a ma;imum rate. #pot'esis is a))epted

group of students did a study on the function of en!ymes as an organic

catalyst that can regulate and increase the rate biochemical reactions in the cell"

#n!ymes are $ery sensiti$e to a change in temperature and functions e%ciently

at an optimum temperature" &esign an e'periment to study the e(ect of

di(erent temperatures on the acti$ity of sali$ary amylase on starch"

The planning of your e'periment must include the following aspects)

* Statement of identi+ed problem

* Objecti$e of study

* ariables

* Statement of $ariables

* -ist of materials and apparatus

* Techni.ue used

* #'perimental procedure

* Presentation of data

* /onclusion


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Sample answer:

Problem Statement

How does di(erent temperatures (ect the acti$ity of sali$ary amylase0

Aim:To study the e(ect of di(erent temperatures on the acti$ity of sali$ary

amylase "

Variables:

1anipulated $ariables) Temperature"

2esponding $ariables) Time taken for hydrolysis of starch to be completed33 2ate

of reaction"

/ontrolled $ariable) en!yme concentration

Hypothesis:

The rate of reaction of sali$ary amylase on starch increases with an increase in

temperature until it reaches the optimum temperature"

Apparatus & Materials:

Test tube, a dropper, a stopwatch, beakers, a thermometer, a white tile, a

Bunsen burner, a tripod stand, test4tube rack, a wire gau!e"

5 6 starch solution, sali$ary amylase solution, ice cubes, distilled water, iodine

test solution"

e!hni"ue

1easure and record the time taken for hydrolysis of starch to be completed using

stop!atchPro!edure:

5" /ollect 7 ml of sali$a and dilute it with 7 ml of distilled water and labelled test

tube 8"

9" Put 5 ml sali$a in each test tube labelled , B, /, & and #"

:" Pour 7 ml of 5 6 starch solution into each test tube labelled , B, /, & and #"

;" =/ respecti$ely"

5=" The results are recorded in a table"


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#esult:

/onclusion)

The rate of increase of en!yme action increases with an increase in temperature

until it reaches the optimum temperature" Hypothesis accepted

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Eks Bab4 Kepekatan Enzim