Amine-reactive PESͷಛͱम০खॱ NEXT Amine-reactive PES Amine-reactive PES H 2 O 2 H 2 O O2 ۃϝ σ Ο Τ ʔ λ ʔ ʢ Խ ܕʣ ϝ σ Ο Τ ʔ λ ʔ ʢ ܕݩʣ ۃୈ2ϝ σ Ο Τ ʔ λ ʔ ʢ Խ ܕʣ ϝ σ Ο Τ ʔ λ ʔ ʢ ݩ ܕʣ త ͳ ࢠҠ ಈ ୈ2.5 ۃత ͳ ࢠҠ ಈ Ҽ ࢠFAD, NAD(P), PQQ ૉ ۃجੜ Ҽ ࢠҼ ࢠʢԽܕʣ ʢԽܕʣ ʢܕݩʣ ʢܕݩʣ Ҽ ࢠҼ ࢠୈ3ୈ1e - e - e - e - 標識可能な 新規電子メディエーター Amine-reactive PESΞϛϊجԠΛ༗Δ৽نࢠϝσΟΤʔλʔͰɻόΠΦηϯαʔͰ༻ΕΔԽݩૉ ߹ΔͱʹΑΓɺόΠΦηϯαʔͷԠ༻ظΕ·ɻ ຊΛ༻म০ૉʹɺಛڐग़ئதͰɻ Amine-reactive PESΞϛϊجԠΛ༗Δ৽نࢠϝσΟΤʔλʔͰɻόΠΦηϯαʔͰ༻ΕΔԽݩૉ ߹ΔͱʹΑΓɺόΠΦηϯαʔͷԠ༻ظΕ·ɻ ຊΛ༻म০ૉʹɺಛڐग़ئதͰɻ छૉʹम০ࢠϝσΟΤʔλʔɺૉͷಇΛҡʹۃࢠΛडͰΔͱใࠂΕ·ɻ ߴՁͳଐࡨମΛஔΔͱۃΒͷࢠϝσΟΤʔλʔͷ࿙ग़ͷՌظΕ·ɻ ΫϩϯΞϯϖϩϝτϦʔʢ0 mV vs Ag/AgClʣ ߟจ ݙʢXXIV International Symposium on Bioelectrochemistry and Bioenergetics, 3-7 July, 2017, Lyon, Franceʣ Stefano Ferri, Katsuhiro Kojima and Koji SODE, “Review of Glucose Oxidases and Glucose Dehydrogenases: A Bird’ s Eye View of Glucose Sensing Enzymes”. Journal of Diabetes Science and Technology, 2011, 5 (5),1068 ଌఆɿάϧίʔεଌఆ CH3 N+ N O O O O N O 0 100 200 300 電流値 グルコース濃度(mg/ml) جੜ Ҽ ࢠϝσΟΤʔλʔ ۃ ۃࢠϝσΟΤʔλʔम০ૉʢGDHʣΛ༻ΔͱͰະम০GDHͰෆՄͳάϧίʔεೱͷఆٴͼϞχλϦϯάଌ ఆՄͰɻ 未標識FADGDH PES標識FADGDH CH 2 OH OH OH OH CH 2 OH OH OH O glucose glucono-δ-lactone OH FAD GDH Ox Red Red Ox stirrer 100 mM P.P.B (pH 7.0) WE RE CE Potentiostat PES 㟁ᴟ O OH O e - 0 20 40 60 80 100 ਗ਼க ৎ/ী ࢢࢥࢫῧຍ ৽ͷࢠϝσΟΤʔλʔ
（XXIV International Symposium on Bioelectrochemistry and
Bioenergetics, 3-7 July, 2017, Lyon, France）
Stefano Ferri, Katsuhiro Kojima and Koji SODE, “Review of
Glucose Oxidases and Glucose Dehydrogenases: A Bird’ s Eye View of
Glucose Sensing Enzymes”. Journal of Diabetes Science and
Technology, 2011, 5(5),1068
