Department of Chemistry E-mail: arriaga@umn.edu University of Minnesota Office phone: (612)-624-8024 Minneapolis, MN 55455 Fax: (612)-626-7541

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Edgar Augusto Arriaga Educational History • Postdoctoral Research Associate, Department of Chemistry, University of Alberta, Edmonton,

Alberta, Canada, 1992-1994 (Advisor: Norman Dovichi). • Postdoctoral Research Associate, Department of Physiology, University of Kansas Medical

Center, Kansas City, Kansas, 1990-1992 (Advisors: Dennis Valenzeno and Merrill Tarr). • Ph.D. in Analytical Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada, 1990 (Advisor:

Dr. Roger Stephens). • Licenciatura en Química, Universidad del Valle de Guatemala, Guatemala City, Guatemala, 1985

(Advisors: Sergio Aragon, SFSU and Elvira de Mejia, UIUC). Employment • Professor of Chemistry, Department of Chemistry, University of Minnesota

Minneapolis, MN, August 2010 to present • Visiting Professor, Molecular and Cell Biology, University of California

Berkeley, CA, January 2013 to present • Visiting Professor, Proteomics and Signal Transduction, Max-Planck Institut für Biochemie,

Martinsried, Germany, 2005 - 2006 • Associate Professor of Chemistry, Department of Chemistry, University of Minnesota,

Minneapolis, MN, August 2004 - 2010 • Assistant Professor of Chemistry, Department of Chemistry, University of Minnesota

Minneapolis, MN, August 1998 - 2004. • Research Associate, Department of Chemistry, University of Alberta

Edmonton, Alberta, 1994 -1998 • Production Manager, Colortec S.A.

Guatemala City, Guatemala, 1986 • Quality Control Director, Colortec S.A.

Guatemala City, Guatemala, 1985 • Undergraduate Research Assistant, Pincasa S.A., Universidad del Valle, Guatemala City,

Guatemala, 1983. Other Professional Appointments at the University of Minnesota • Member, Graduate Faculty of the Masters of Biological Sciences, 2011 to present • Member, Graduate faculty of Gerontology, 2009 to present • Senior Member, Graduate Faculty of the Department of Biochemistry, Molecular Biology, and

Biophysics, 2008 to present • Mentor, NIH Functional Proteomics of Aging Training Grant, 2008 to present • Member, Cancer Center, 2006 to present • Member, Graduate Faculty of the Integrated Biosciences Program, 2005 to present • Member, Advisory Board to the Center for Mass Spectrometry and Proteomics, 2004 to present • Member, Graduate Faculty of the Department of Biomedical Engineering, 2001 to present • Preceptor, MD/Ph.D. Program, Medical School, 2002 to present • Mentor, NIH Biotechnology Training Grant, 1999 to present • Mentor, NIH Chemistry-Biology Interface Training Grant, 1999 to present • Member, Molecular Nanosciences Alliance for Interdisciplinary Studies and Activities, 2001 – 2005

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Other Professional Appointments External to the University of Minnesota • Member, American Chemical Society - Committee on Professional Training, 2012 to present • Chartered Member, NIH National Institute in Aging – Panel B Review Study Section, 2012 to present • Chartered Member, NIH Emerging Biotechnologies Review Study Section, 2007 - 2011 • Guest Editor, special issue on “Analytical Tools for Cell Research,” Analytical and Bioanalytical

Chemistry, 2009 - 2010 • Member, Scientific Advisory Board, Microscale Bioseparations & Methods for Systems Biology

Conference (MSB, previously HPCE), 2008 - 2009 • Guest Editor, special issue on “Single Cell Analysis,” Analytical and Bioanalytical Chemistry 387,

2006 - 2007 • Advisory Board, Analytical and Bioanalytical Chemistry, 2004 to present • A-Page Advisory Panel, Analytical Chemistry, 2001 - 2003 Honors and Recognition • Fesler-Lampert Chair in Aging Studies, Center on Aging and the Graduate School, University of Minnesota, 2007 - 2008 • KO2 NIH Career Award, 2003 - 2008 • Distinguished Mentor Award, President’s Distinguished Faculty Mentor Program, University of

Minnesota, 2001 • Research Innovation Award, Research Corporation, 2000 • NATO Scholarship, ASI School of Photobiological Techniques, Kingston, ON, Canada, 1990 • Dalhousie Graduate Fellowship, Dalhousie University, Halifax, NS, Canada, 1986 - 1990 • Distinguished Student Honor, Universidad del Valle, Guatemala City, Guatemala, 1984 • Undergraduate Scholarship, Universidad del Valle, Guatemala City, Guatemala, 1981 - 1982 University of Minnesota Affiliations • Center on Aging (2008 to present) • Biochemistry, Molecular Biology and Biophysics Graduate Program (2008 to present) • Biomedical Engineering Graduate Program (2001 to present) • Cancer Center (2006 to present ) • Integrated Biosciences Program (2005 to 2011) • NIH, Biotechnology Training Grant (1999 to present) • NIH, Chemistry-Biology Interface Training Grant (1999 to present) • NIH, Functional Proteomics of Aging (2008 to present) • Molecular Nanosciences Alliance for Interdisciplinary Studies and Activities, 2001 – 2005 • Scientists in Aging Research (2000 to present) Research Interests • Development and engineering of analytical chemistry techniques for subcellular analysis • Molecular characterization and quantification in aging biological systems • Characterization of biological heterogeneity associated with aging and disease conditions

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Publications

Peer Reviewed Publications: 122 1. Arriaga, E. A.; Stephens, R. "Detection of Structured Interferences in a.c. Zeeman Atomic Absorption by

Harmonic Analysis", Spectrochim. Acta 44B, 867-76 (1989).

2. Valenzeno, D. P.; Arriaga, E.; Trank, J.; Tarr, M. "Membrane Potential Can Influence the Rate of Membrane Photomodification", Photochem. Photobiol. 57, 996-9 (1993).

3. Arriaga, E.; Chen, D. Y.; Cheng, X. L.; Dovichi, N. J. "High Efficiency Filter Fluorometer for Capillary Electrophoresis and Its Application to Fluorescein Thiocarbamyl Amino Acids", J. Chromatogr., A 652, 347-53 (1993).

4. Tarr, M.; Arriaga E.; Goertz, K. K.; Valenzeno, D. P. "Properties of Cardiac ILeak Induced by Photosensitizer-Generated Reactive Oxygen", Free Radical Biol. Med. 16(4), 477-84 (1994).

5. Arriaga, E.; Frolov, A.; Tarr, M.; Valenzeno, D. P. "Membrane Ionic Current Photomodification by Rose Bengal and Menadione: Role of Singlet Oxygen", Photochem. Photobiol. 59(6), 637-42 (1994).

6. Best, N.; Arriaga, E.; Chen, D. Y.; Dovichi, N. J."Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis", Anal. Chem. 66(22), 4063-7 (1994).

7. Figeys, D.; Arriaga, E.; Renborg, A.; Dovichi, N. J. "Use of the Fluorescent Intercalating Dyes POPO-3, YOYO-3 and YOYO-1 for Ultrasensitive Detection of Double-Stranded DNA Separated by Capillary Electrophoresis with Hydroxypropylmethyl Cellulose and Non-Cross-Linked Polyacrylamide", J. Chromatogr., A 669, 205-16 (1994).

8. Lu, H.; Arriaga, E.; Chen, D. Y.; Starke, H.; Dovichi, N. J. "High-Speed and High-Accuracy DNA Sequencing by Capillary Gel Electrophoresis in a Simple, Low Cost Instrument: Two-Color Peak-Height Encoded Sequencing at 40o C", J.Chromatogr. A 680, 497-501 (1994).

9. Lu, H.; Arriaga, E.; Chen, D. Y.; Figeys, D.; Dovichi, N. J. "The Activation Energy of Single-Stranded DNA Moving through Cross-Linked Polyacrylamide Gels at 300 V/Cm: Effect of Temperature on Sequencing Rate in High-Electric-Field Capillary Gel Electrophoresis", J. Chromatogr., A 680, 503-10 (1994).

10. Tarr, M.; Arriaga, E.; Valenzeno, D. "Progression of Cardiac Potassium Current Modification after Brief Exposure to Reactive Oxygen", J. Mol. Cell. Cardiol. 27(5), 1099-109 (1995).

11. Arriaga, E. A.; Zhang, Y.; Dovichi, N. J. "Use of 3-(P-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQ) to Label Amino Acids for High Sensitivity Fluorescence Detection in Capillary Electrophoresis", Anal. Chim. Acta 299, 319-26 (1995).

12. Craig, D.; Arriaga, E.; Banks, P.; Zhang, Y.; Renborg, A.; Palcic, M. M.; Dovichi, N. J. "Fluorescently-Based Enzymatic Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection for the Determination of a Few B-Galactosidase Molecules", Anal. Biochem. 226, 147-53 (1995).

13. Pinto, D. M.; Arriaga, E. A.; Sia, S.; Li, Z.; Dovichi, N. J. "Solid-Phase Fluorescent Labeling Reaction of Picomole Amounts of Insulin in Very Dilute Solutions and Their Analysis by Capillary Electrophoresis", Electrophoresis 16, 534-40 (1995).

14. Zhang, Y.; Arriaga, E.; Diedrich, P.; Hindsgaul, O.; Dovichi, N.J. "Nanomolar Determination of Aminated Sugars by Capillary Electrophoresis", J. Chromatogr., A 716, 221-29 (1995).

15. Figeys, D.; Ahmadzadeh, H.; Arriaga, E.; Dovichi, N. J. "Pseudo-Coulometric Loading in Capillary Electrophoresis DNA Sequencing", J. Chromatogr., A 744, 325-31 (1996).

16. Craig, D. B.; Arriaga, E.; Wong, J. C. Y.; Lu, H.; Dovichi, N. J. "Studies on Single Alkaline Phosphatase Molecules: Reaction Rate and Activation Energy of a Reaction Catalyzed by a Single Molecule and the Effect of Thermal Denaturation--The Death of an Enzyme", J. Am. Chem. Soc. 118 (22), 5245 -53 (1996).

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17. Pinto, D. M.; Arriaga, E. A.; Craig, D.; Angelova, J.; Sharma, N.; Ahmadzadeh, H.; Dovichi, N. J. "Picomolar Assay of Native Proteins by Capillary Electrophoresis Precolumn Labeling, Submicellar Separation, and Laser-Induced Fluorescence Detection", Anal. Chem. 69, 3015-21 (1997).

18. Craig, D. B.; Arriaga, E.; Wong, J. C. Y.; Lu, H.; Dovichi, N. J. News & Features: "The Life and Death of a Single Enzyme Molecule", Anal. Chem. 70, 39A-43A (1998).

19. Stathakis, C.; Arriaga, E. A.; Lewis, D. F.; Dovichi, N. J. "Cationic and Anionic Polymeric Additives for Wall Deactivation and Selectivity Control in the Capillary Electrophoretic Separation of Proteins in Food Samples", J. Chromatogr., A 817, 227-32 (1998).

20. Stathakis, C.; Arriaga, E. A.; Dovichi, N. J. "Protein Profiling Employing Capillary Electrophoresis with Dendrimers as Pseudostationary Phase Media", J. Chromatogr., A 817, 233-8 (1998).

21. Lee, I-H; Pinto, D.; Arriaga, E. A.; Dovichi, N. J. "Picomolar Analysis of Proteins Using Electrophoretically Mediated Microanalysis and Capillary Electrophoresis with Laser Induced Fluorescence Detection", Anal. Chem. 70, 4546-8 (1998).

22. Craig, D. B.; Polakowski, R.; Wong, J. C. Y; Arriaga, E. A.; Ahmadzadeh, H.; Stathakis, C.; Dovichi, N. J. "SDS-Capillary Electrophoresis of Proteins in a Sieving Matrix Utilizing Two-Spectral Channel Laser-Induced Fluorescence Detection", Electrophoresis 19, 2175-78 (1998).

23. Ocvirk, G.; Salimi-Moosavi, H.; Szarka, R. J.; Arriaga, E. A.; Andersson, P. E.; Smith, R.; Dovichi, N. J.; Harrison, D. J. "Single Cell Enzymatic Analysis on a Microchip: Lysing of Single Cells and Identification of Their b-galactosidase Activity", Proceedings of Third Conference on Micro-total Analysis System, Banff, Alberta, Canada (1998).

24. Le, X. C.; Tan, W.; Scaman, C. H.; Szpacenko, A.; Arriaga, E.; Zhang, Y.; Dovichi, N. J.; Hindsgaul, O.; Palcic, M. M. "Single Cell Studies of Enzymatic Hydrolysis of a Tetramethylrhodamine Labeled Triglucoside in Yeast", Glycobiology 9, 219-25 (1999).

25. Krylov, S. N.; Zhang, Z.; Chan, N. W. C.; Arriaga, E .A.; Palcic, M. M.; Dovichi, N. J. "Correlating Cell Cycle with Metabolism in Single Cells: Combination of Image and Metabolic Cytometry", Cytometry 37, 14-20 (1999).

26. Krylov, S. N.; Arriaga, E.; Chan, N.W.C.; Dovichi, N.; Palcic, M.M. "Metabolic Cytometry: Monitoring Oligosaccharide Biosynthesis in Single Cells" in Sialobiology and Other Novel Forms of Glycosylation. Y. Inoue, Y.C. Lee, and F.A. Troy II, Eds., Gakushin Publishing, Osaka, Japan, 259-64 (1999).

27. Zhang, Z.; Krylov, S.; Arriaga, E. A.; Polakowski, R; Dovichi, N. J. "One-Dimensional Protein Analysis of an HT29 Human Colon Adenocarcinoma Cell", Anal. Chem. 72, 318-22 (2000).

28. Krylov, S. N.; Starke, D. A.; Arriaga, E. A.; Zhang, Z.; Chan, N. W. C.; Palcic, M. M., Dovichi, N. J. Technical Notes: "Instrumentation for Chemical Cytometry", Anal. Chem. 72, 872-7 (2000).

29. Krylov, S. N.; Arriaga, E. A.; Zhang, Z.; Chan, N. W. C.; Palcic, M. M; Dovichi, N. J. "Single-Cell Analysis Avoids Sample Processing Bias", J. Chromatogr., B: Biomed. Sci. Appl. 741, 31-5 (2000).

30. Krylov, S. N.; Arriaga, E. A.; Chan, N. W. C.; Dovichi, N. J.; Palcic, M. M. "Metabolic Cytometry: Monitoring Oligosaccharide Biosynthesis in Single cells by Capillary Electrophoresis", Anal. Biochem. 283 (2), 133-5 (2000).

31. Duffy, C. F.; Gafoor, S.; Richards, D. F.; Admadzadeh, H.; O’Kennedy, R.; Arriaga, E. A. "Determination of Properties of Individual Liposomes by Capillary Electrophoresis with Post-Column Laser-Induced Fluorescence Detection", Anal. Chem. 73, 1855-61 (2001).

32. Strack, A.; Duffy, C. F.; Malvey, M.; Arriaga, E. A. "Individual Mitochondrion Characterization: A Comparison of Classical Assays to Capillary Electrophoresis with Laser-Induced Fluorescence Detection", Anal. Biochem. 294, 141-7 (2001).

33. Boulat, O.; McLaren, D. G.; Arriaga, E. A.; Chen, D. D. Y. "Separation of Free Amino Acids in Human Plasma by Capillary Electrophoresis with Laser Induced Fluorescence: Potential for Emergency Diagnosis of Inborn Errors of Metabolism", J. Chromatogr., B: Biomed. Sci. Appl. 754, 217-28 (2001).

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34. Duffy, C. F.; Fuller, K.; Malvey, M.; O’Kennedy, R.; Arriaga, E. A. "Determination of Electrophoretic Mobility Distributions through the Analysis of Individual Mitochondrial Events by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", Anal. Chem. 74, 171-76 (2002).

35. Anderson, A.; Gergen, J.; Arriaga, E. A. "Detection of Doxorubicin and Metabolites in Cell Extracts and in Single Cells by Capillary Electrophoresis with Laser-Induced Fluorescence Detection". J. Chromatogr., B 769, 97-106 (2002).

36. Fuller, K. M.; Duffy, C. F.; Arriaga, E. A. "Determination of the Cardiolipin Content of Individual Mitochondria by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", Electrophoresis 23, 1571-76 (2002).

37. Duffy, C. F.; McEathron, A.; Arriaga, E. A. "Determination of Individual Microsphere Properties by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", Electrophoresis 23, 2040-47 (2002).

38. Gunasekera, N.; Musier-Forsyth, K.; Arriaga, E. A. "Electrophoretic Behavior of Individual Nuclear Species as Determined by Capillary Electrophoresis with Laser-induced Fluorescence Detection", Electrophoresis 23, 2110-16 (2002).

39. Anderson, A. B.; Ciriacks, C. M.; Fuller, K. M.; Arriaga, E. A. "Distribution of Zeptomole-Abundant Doxorubicin Metabolites in Subcellular Fractions by Capillary Electrophoresis with Laser-induced Fluorescence Detection", Anal. Chem. 75, 8-15 (2003).

40. Fuller, K.; Arriaga, E. A. “Advances in Single Mitochondria Analysis", Invited review, Curr. Opin. Biotechnol. 14, 35-41 (2003).

41. Fuller, K.; Arriaga, E. A. "Analysis of Individual Acidic Organelles by Capillary Electrophoresis with Laser-Induced Fluorescence Detection Facilitated by the Endocytosis of Fluorescently Labeled Microspheres", Anal. Chem. 75, 2123-2130 (2003).

42. Presley, A. D.; Fuller, K. M.; Arriaga, E. A. "MitoTracker Green Labeling of Mitochondrial Proteins and Their Subsequent Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", J. Chromatogr., B. 793, 141-150 (2003).

43. Pinto, D.; Arriaga, E. A.; Schoenherr, R.M.; Chou, S. S.-H.; Dovichi, N. J. "Kinetics and Apparent Activation Energy of the Reaction of the Fluorogenic Reagent 5-Furoylquinoline-3-carboxaldehyde with Ovalbumin", J. Chromatogr., B 793, 107-114 (2003).

44. Xiong, G.; Aras, O.; Shet, A.; Key, N.; Arriaga, E. A. "Analysis of Individual Platelet-Derived Microparticles, Comparing Flow Cytometry and Capillary Electrophoresis with Laser-Induced Fluorescence Detection", Analyst. 128, 581-588 (2003).

45. Ahmadzadeh, H.; Johnson, R. D.; Thompson, L.; Arriaga, E. A. "Direct Sampling from Muscle Cross Sections for Electrophoretic Analysis of Individual Mitochondria", Anal. Chem. 76, 315-321 (2004).

46. Gunasekera, N.; Olson, K. J.; Musier-Forsyth, K.; Arriaga, E. A. "Capillary Electrophoretic Separation of Nuclei Released from Single Cells", Anal. Chem. 76, 655-662 (2004).

47. Ocvirk, G.; Salimi-Moosavi, H.; Szarka, R. J.; Arriaga, E. A.; Andersson, P. E.; Smith, R.; Dovichi, N. J.; Harrison D.J. “b-Galactosidase Assays of Single Cell Lysates on a Microchip: A Complementary Method for Enzymatic Analysis of Single Cells,” Proc. IEEE 92, 115-25 (2004).

48. Gunasekera, N.; Xiong, G.; Musier-Forsyth, K.; Arriaga, E. A. “A Capillary Electrophoretic Method For Monitoring the Presence of a-Tubulin in Nuclear Preparations”, Anal. Biochem. 330, 1-9 (2004).

49. Fuller, K.; Arriaga, E. A. "Capillary Electrophoresis Monitors Changes in the Electrophoretic Behavior of Mitochondrial Preparations", J. Chromatogr., B. 806, 151-9 (2004).

50. Anderson, A. B.; Arriaga, E. A. “Subcellular Metabolite Profiles of the Parent CCRF-CEM and the Derived CEM/C2 Cell Lines after Treatment with Doxorubicin”, J. Chromatogr., B. 808, 295-302 (2004)

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51. Arriaga, E. A. Book review of Monolithic Materials: Preparation, Properties and Applications, Journal of Chromatography Library, Volume 67, F. Svec, T.B. Tennikova, and Z. Deyl, eds., Elsevier: Amsterdam, 2003, in J. Am. Chem. Soc. 126, 9874 (2004).

52. Gunasekera, N.; Lee, W. L.; Kim, S.; Musier-Forsyth, K.; Arriaga, E. A. “Nuclear Localization of Aminoacyl-tRNA Synthetases Using Single-Cell Capillary Electrophoresis Laser-Induced Fluorescence Analysis”, Anal. Chem. 76, 4741-6 (2004).

53. Anderson, A. B.; Xiong, G.; Arriaga, E. A. “Doxorubicin Accumulation in Individually Electrophoresed Organelles”, J. Am. Chem. Soc. 126, 9168-9 (2004).

54. Ahmadzadeh, H.; Dua, R.; Presley, A. D.; Arriaga, E. A. “Automated Analysis of Individual Particles Using a Commercial Capillary Electrophoresis System”, J. Chromatogr., A. 1064, 107-14 (2005).

55. Chen, Y.; Walsh, R.; Arriaga, E. A. "Selective Determination of the Doxorubicin Content of Individual Acidic Organelles in Impure Subcellular Fractions", Accelerated Article, Anal. Chem. 77, 2281-7 (2005).

56. Xiong, G.; Chen, Y.; Arriaga, E. A. "Measuring the Doxorubicin Content of Single Nuclei by Micellar Electrokinetic Capillary Chromatography with Laser-Induced Fluorescence Detection", Anal. Chem. 77, 3488-93 (2005).

57. Olson, K. J.; Ahmadzadeh, H.; Arriaga, E. A. “Within the Cell: Analytical Techniques for Subcellular Analysis”, Anal. Bioanal. Chem. 382, 906-17 (2005).

58. Eder, A. E.; Arriaga, E. A., “Micellar Electrokinetic Capillary Chromatography Reveals Differences in Intracellular Metabolism between Liposomal and Free Doxorubicin Treatment of Human Leukemia Cell Lines”, J. Chromatogr., B.: Anal. Technol. Biomed. Life Sci. 829, 115-22 (2005).

59. Ahmadzadeh, H.; Thompson, L.V.; Arriaga, E.A. “On-Column Labeling for Capillary Electrophoretic Analysis of Individual Mitochondria Sampled from Tissue Cross Sections”, Technical Note, Anal. Bioanal. Chem. 384, 169-74 (2006).

60. Chen, Y.; Arriaga, E.A. “Individual Acidic Organelle pH Measurements by Capillary Electrophoresis”, Anal. Chem. 78, 820-6 (2006).

61. Navratil, M.; Mabbott, G. A.; Arriaga, E.A. “Chemical Microscopy Applied to Biological Systems,” Review, Anal. Chem. 78, 4005-19 (2006).

62. Eder, A.R.; Chen, J.S.; Arriaga, E.A. “Separation of Doxorubicin and Doxorubicinol by Cyclodextrin-Modified Micellar Electrokinetic Capillary Chromatography”, Electrophoresis 27, 3263-70 (2006).

63. Duffy, C. F.; MacCraith, B.; Diamond, D.; O'Kennedy, R.; Arriaga, E.A. “Fast Electrophoretic Analysis of Individual Mitochondria Using Microchip Capillary Electrophoresis with Laser Induced Fluorescence Detection,” Lab Chip 6, 1007-11 (2006). This article was chosen for inclusion in the Royal Society of Chemistry’s Chemical Biology Virtual Journal, Issue 15, 2006.

64. Meany, D.L.; Poe, B.G.; Navratil, M.; Moraes, C.T.; Arriaga, E.A. “Superoxide Released into the Mitochondrial Matrix”, Free Radical Biol. Med. 41, 950-9 (2006).

65. Eder, A.R.; Arriaga, E.A. “Capillary Electrophoresis Monitors Enhancement in Reactive Oxygen Species Production upon Treatment with Doxorubicin”, Chem. Res. Toxicol. 19, 1151-9 (2006).

66. Whiting, C.; Arriaga, E.A. “CE-LIF Analysis of Mitochondria Using Uncoated and Dynamically Coated Capillaries”, Electrophoresis 27, 4523-31 (2006).

67. Poe, B.G.; Navratil, M.; Arriaga, E.A. “Analysis of Subcellular-Sized Particles: Capillary Electrophoresis with Post-Column Laser-Induced Fluorescence Detection versus Flow Cytometry,” J. Chromatogr., A. 1137, 249-55 (2006).

68. Forner, F.; Arriaga, E.A.; Mann, M. “Mild Protease Treatment as a Small-Scale Biochemical Method for Mitochondria Purification and Proteomic Mapping of Cytoplasm-Exposed Mitochondrial Proteins”, J. Proteome Res. 5, 3277-87 (2006).

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69. Ahmadzadeh, H.; Andreyev, D.; Arriaga, E. A.; Thompson, L.V. “Capillary Electrophoresis Reveals Changes in Individual Mitochondrial Particles Associated with Skeletal Muscle Fiber Type and Age,” J. Gerontol. Series A: Biol. Sci. Med. Sci. 61A, 1211-18 (2006).

70. Johnson, R.D.; Navratil, M.; Poe, B.G.; Xiong, G.; Olson, K.J.; Ahmadzadeh, H.; Andreyev, D.; Duffy, C.F.; Arriaga, E.A. “Analysis of Mitochondria Isolated from Single Cells,” Anal. Bioanal. Chem. 387, 107-18 (2007).

71. Poe, B.G.; Navratil, M.; Arriaga, E.A. “Absolute Quantitation of a Heteroplasmic Mitochondrial DNA Deletion Using a Multiplex Three-Primer Real-Time PCR Assay,” Anal. Biochem. 362, 193-200 (2007).

72. Li, D.; Xie, H.; Thompson, L.V.; Arriaga, E.A.; Griffin, T.J. “Identification of Carbonylated Proteins from Enriched Rat Skeletal Muscle Mitochondria Using Affinity Chromatography-Stable Isotope Labeling and Tandem Mass Spectrometry”, Proteomics 7, 1150-1163 (2007)

73. Whiting, C.E.; Arriaga, E.A. “Evaluation of Individual Particle Capillary Electrophoresis Experiments via Quantile Analysis”, J. Chromatogr., A. 1157, 446-53 (2007).

74. Meany, D.L.; Thompson, L.V.; Arriaga, E.A. “Simultaneously Monitoring the Superoxide in the Mitochondrial Matrix and Extramitochondrial Space by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence”, Anal. Chem. 79, 4588-94 (2007).

75. Andreyev, D.; Arriaga, E.A. “Simultaneous Laser-Induced Fluorescence and Scattering Detection of Individual Particles Separated by Capillary Electrophoresis”, Anal. Chem. 79, 5474-8 (2007).

76. Chen, Y.; Xiong, G.; Arriaga, E. “CE Analysis of the Acidic Organelles of a Single Cell”, Electrophoresis 28, 2406-15 (2007).

77. Chen, Y.; Arriaga, E.A. “Individual Electrophoretic Mobilities of Liposomes and Acidic Organelles Displaying pH Gradients Across Their Membranes,” Langmuir 23, 5584-90 (2007).

78. Andreyev, D.S.; Arriaga, E.A. “Fabrication of Perforated Sub-Micron Silica Shells”, Scri. Mater. 57, 957-9 (2007).

79. Navratil, M.; Poe, B.G.; Arriaga, E.A. “Quantitation of DNA Copy Number in Individual Mitochondrial Particles by Capillary Electrophoresis,” Anal. Chem. 79, 7691-9 (2007).

80. Navratil, M.; Terman, A.; Arriaga, E.A. “Giant Mitochondria Do Not Fuse and Exchange Their Contents with Normal Mitochondria”, Exp. Cell Res. 314, 164-72 (2008).

81. Feng, J.; Arriaga, E.A. “Quantification of Carbonylated Proteins in Rat Skeletal Muscle Mitochondria Using Capillary Sieving Electrophoresis with Laser-Induced Fluorescence Detection”, Electrophoresis 29, 475-82 (2008).

82. Whiting, C.E.; Dua, R.; Duffy, C.F; Arriaga, E.A. “The Statistical Limitations of Sampling Individual Particles on Microfluidic Devices with Orthogonal Laser Induced Fluorescence Detection”, Electrophoresis 29, 1431-40 (2008).

83. Wang, Y; Arriaga, E.A. “Monitoring Incorporation, Transformation and Subcellular Distribution of N-L-leucyl-doxorubicin in Uterine Sarcoma Cells Using Capillary Electrophoretic Techniques”, Cancer Lett. (Shannon, Irel.) 262, 123-32 (2008).

84. Kostal, V.; Arriaga, E.A. “Recent Advances in the Analysis of Biological Particles by Capillary Electrophoresis”, Review, Electrophoresis 29, 2578-86 (2008).

85. Kostal, V.; Katzenmeyer, J. B.; Arriaga, E.A. “Capillary Electrophoresis in Bioanalysis”, (Invited Review) Anal. Chem. 80, 4533-50 (2008).

86. Feng, J.; Navratil, M.; Thompson, L.V.; Arriaga, E.A. “Estimating Relative Carbonyl Levels in Muscle Microstructures by Fluorescence Imaging”, Anal. Bioanal. Chem. 391, 2591-8 (2008).

87. Feng, J.; Xie, H.; Meany, D.L; Thompson, L.V.; Arriaga, E.A.; Griffin, T.J. “Quantitative Proteomic Profiling of Muscle-Dependent and Age-Dependent Protein Carbonylation in Rat Skeletal Muscle Mitochondria”, J. Gerontol.: Biol. Sci. 63A, 1137-52 (2008).

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88. Feng, J.; Navratil, M.; Thompson, L.V.; Arriaga, E.A. “Principal Component Analysis Reveals Age-Related and Muscle-Type-Related Differences in Protein Carbonyl Profiles of Muscle Mitochondria”, J. Gerontol.: Biol. Sci. 63A, 1277-88 (2008). (PMC3038117)

89. Arriaga, E.A. “Determining Biological Noise Via Single Cell Analysis,” Invited Review, Anal. Bioanal. Chem. 393, 73-80 (2009). (PMC3035192)

90. Wang, Y.; Hong, J.; Cressman, E.N.K.; Arriaga, E.A. “Direct Sampling from Human Liver Tissue Cross-sections for Electrophoretic Analysis of Doxorubicin” Anal. Chem. 81, 3321-8 (2009). (PMC2675659)

91. Xu, X.; Arriaga, E.A. “Qualitative Determination of Superoxide Release at Both Sides of the Mitochondrial Inner Membrane by Capillary Electrophoretic Analysis of the Oxidation Products of Triphenylphosphonium Hydroethidine”, Free Radical Biol. Med. 46, 905-13 (2009).

92. Davis, J.M; Arriaga, E. A. "Evaluation of Peak Overlap in Migration-Time Distributions Determined by Organelle Capillary Electrophoresis: Type-II Error Analogy Based on Statistical-Overlap Theory”, J. Chrom. A, 6335-6342 (2009). (PMC2779522)

93. Kostal, V.; Fonslow, B.; Arriaga, E.A.; Bowser, M.T. "Fast Determination of Mitochondria Electrophoretic Mobility Using Micro-Free Flow Electrophoresis", Anal. Chem. 80, 3182-3189 (2009).

94. Davis, J.M; Arriaga, E. A. "Estimation of Migration-time and Mobility Distributions in Organelle Capillary Electrophoresis with Statistical-Overlap Theory”, Anal. Chem. 82, 307–315 (2010). (PMC2803746)

95. Terman, A.; Brunk, U.; Kurz, T.; Arriaga, E.A.; Narvratil, M. “Mitochondrial Turnover and Aging of Long-Lived Postmitotic Cells. The Mitochondrial-Lysosomal Axis Theory of Aging”, Invited Review, Antioxidants and Redox Signaling 12, 503-35 (2010). (PMID1965071)

96. Curtis, J.M.; Grimsrud, P.A.; Wright, W.S.; Xu, X.; Foncea, R.E.; Graham, D.W.; Brestoff, J.R.; Wiczer, B.M.; Ilkayeva, O.; Cianflone, K.; Muoio, D.E.; Arriaga, E.A.; Bernlohr, D.A. "Down Regulation of Adipose Glutathione S-Transferase Leads to Increased Protein Carbonylation, Oxidative Stress and Mitochondrial Dysfunction", Diabetes 59,1132-1142 (2010). (PMC2857893)

97. Poe III, B.G.; Duffy, C.F.; Greminger, M.A.; Nelson, B.J.; Arriaga, E.A. “Detection of Heteroplasmy in Individual Mitochondrial Particles”, Anal. Bioanal. Chem. 397, 3397–3407 (2010). (PMC3035356)

98. Xu, X.; Thompson, L.V.; Navratil, M., Arriaga E.A. “Analysis of Superoxide Production in Single Skeletal Muscle Fibers”, Anal. Chem. 82, 4570-4576 (2010).

99. Xu, X.; Arriaga, E.A. “Quantitative Chemical Cytometry Determines the Superoxide Levels in the Mitochondrial Matrix of Single Myoblasts”, Anal. Chem. 82, 6745-6750 (2010).

100. Katzenmeyer, J.B.; Eddy, C.V.; Arriaga, E.A. “Tandem Laser-Induced Fluorescence and Mass Spectrometry Detection for High-Performance Liquid Chromatography Analysis of the in Vitro Metabolism of Doxorubicin”, Anal. Chem., 82, 8113-8120 (2010). (PMC2989425)

101. Xu, X.; Chen, C-n.; Arriaga, E.A.; Thompson, L.V. “Asymmetric superoxide release inside and outside the mitochondria in skeletal muscle under conditions of aging and disuse”, Journal of Applied Physiology. 109, 1133-1139 (2010).

102. Wolken, G.G.; Kostal, V.; Arriaga, E.A. “Capillary Isoelectric Focusing of Individual Mitochondria", Anal. Chem., 83 (2), 612-618 (2011). (PMC3021621)

103. Kostal, V.; Arriaga, E. A. “"Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton", Anal. Chem. 83 (5), 1822 – 1829 (2011).

104. Kostal, V.; Levar, K.; Swift, M.; Skillrud, E.; Chapman, M.; Thompson, L.V.; Arriaga, E.A. “Semi-automated image analysis: Detecting carbonylation in subcellular regions of skeletal muscle”, Anal. Bioanal. Chem. 400, 213-222 (2011). (PMC3066023)

105. Wang, Y.; Katzenmeyer, J.B.; Arriaga, E.A. “Combination of Micellar electrokinetic and high-performance liquid chromatographies to assess age-related changes in the in vitro metabolism of Fischer 344 rat liver”, J. Gerontol.: Biol. Sci. 66 (9), 935-43 (2011).

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106. Satori, C. P.; Kostal, V.; Arriaga, E.A. “Individual organelle pH determinations of magnetically-enriched endocytic organelles via laser-induced fluorescence detection”, Anal. Chem. 83 (19), 7331-9 (2011). (PMC3184341)

107. Wang, Y.; Taylor, H.T.; Arriaga, E.A. “Analysis of the Bioactivity of Magnetically Immunoisolated Peroxisomes”, Anal. Bioanal. Chem. 402(1), 41-9 (2012). (Paper at the Forefront) (PMC3251717)

108. Cabrera, J. A.; Ziemba, E. A., Kelly, R. F.; Sikora, J.; Colbert, R.; Kuskowski, M.; Arriaga, E. A.; Sluiter, W.; Duncker, D. J.; Ward, H. B.; McFalls, E. O. “Uncoupling Protein-2 Expression and Effects on Mitochondrial Membrane Potential and Oxidant Stress in Heart Tissue”, Translational Research, 159, 383-90 (2012). (PMC3328031)

109. Jagtap, P; Bandhakavi, R.; Higgins, L.; McGowan, T.; Sa, R.; Stone, M.D.; Chilton, J.; Arriaga, E.A.; Seymour, S.L.; Griffin, J.T. “Workflow for analysis of high mass accuracy salivary dataset using MaxQuant and ProteinPilot search algorithm”, Proteomics, 12, 1726-1730 (2012). (PMID: 22623410)

110. Curtis, J.M.; Hahn, W.S.; Long, E.K.; Burril, J.; Arriaga, E.A.; Bernlohr, D.A. “Protein Carbonylation and metabolic Control Systems”, Trends in Endocrinology and Metabolism, 23, 399-406 (2012). (PMC3408802).

111. Curtis, J.M.; Hahn, W.S.; Stone, M.D.; Inda, J.J.; Droullard, D.J.; Kuzmicic, J.P.; Donoghue, M.A.; Long, E.K.; Armien, A.G.; Lavandero, S.; Arriaga, E.A.; Griffin, T.G.; Bernlohr, D.A., "Protein carbonylation and adipocyte mitochondria function", Journal of Biological Chemistry, 287, 32967-80 (2012) (PMC3463318)

112. Satori, C.P.; Kostal, V.; Arriaga, E.A. "Review on Recent Advances in the Analysis of Isolated Organelles", Anal. Chim. Acta, 753, 8-18 (2012). (PMC3484375)

113. Wolken, G. G.; Fossen, B. J.; Noh, A.; Arriaga, E. A. “Predicting Isoelectric Points of Non-functional Mitochondria from Monte Carlo Simulations of Surface Compositions”, Langmuir, 29, 2700-7 (2013). (PMC3582746)

114. Donoghue, M.A.; Xu, X.; Bernlohr, D.A.; Arriaga, E.A. “Capillary electrophoretic analysis of hydroxyl radicals produced by respiring mitochondria”, Anal. Bioanal. Chem., 405(18), 6053-60 (2013). (PMC3696420)

115. Satori, C. P.; Henderson, M. M.; Krautkramer, E. A.; Kostal, V.; Distefano, M. D.; Arriaga, E. A. “Bioanalysis of eukaryotic organelles”, Chemical Reviews, 113(4), 2733-811 (2013). (PMC3676536)

116. Satori, C.P.; Arriaga, E.A. “Describing Autophagy via Analysis of Individual Organelles by Capillary Electrophoresis with Laser Induced Fluorescence Detection”, Anal. Chem. 85(23), 11391-400 (2013), (PMC3184341)

117. Ro, S.-H.; Jung, C.H.; Hahn, W.S.; Xu, X.; Kim, Y.-M.; Yun, Y.S.; Park, J.-M.; Kim, K.H.; Seo, M.; Ha, T.-Y.; Arriaga, E.A; Bernlohr, D.A.; Kim, D.-H. “Distinct functions of ULK1 and ULK2 in the regulation of lipid metabolism in adipocytes”, Autophagy 9(12), 2103-14 (2013). (PMC in Process)

118. Taylor, T. H.; Frost, N. W.; Bowser, M. T.; Arriaga, E. A. “Analysis of Individual Mitochondrion via Fluorescent Immunolabeling with Anti-TOM 22 Antibodies”, Anal. Bioanal. Chem., 406(6), 1683-91 (2014). (PMC4332841)

119. Satori, C. P.; Meyer, B.; Arriaga, E. A. “Monitoring Subcellular Biotransformation of N-L-Leucyldoxorubicin by Micellar Electrokinetic Capillary Chromatography Coupled to Laser Induced Fluorescence Detection”, Anal. Bioanal. Chem. 406(9-10), 2389-97 (2014). (PMID: 24573576).

120. Luo, J.; Abdallah, B.G.; Wolken, G.G.; Arriaga, E.A.; Ros, A. “Insulator-based dielectrophoresis of mitochondria”, Biomicrofluidics, 8(2), 021801 (2014). (PMC4056684).

121. Hahn W.S; Kuzmicic, J.; Burrill, J.B.; Donoghue, M.A.; Jensen, M.D.; Lavandero, S.; Arriaga; E.A.; Bernlohr, D.A “Pro-inflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress and dynamics”, Am. J. Physiol. Endocrinology and Metabolism, 306(9), E1033-45 (2014). (PMC in process)

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122. Wolken, G.G.; Arriaga, E.A. “Simultaneous Measurement of Individual Mitochondrial Membrane Potential and Electrophoretic Mobility by Capillary Electrophoresis”, Anal. Chem. 86(9), 4217-26 (2014). (PMC4018156).

123. Kumar, S.; Wolken, G.G.; Wittenberg, N.J.; Arriaga, E.A.; Oh, S.-H. “Nanohole array-directed trapping of mammalian mitochondria enabling single organelle analysis”, Anal. Chem. 87, 11973-11977 (2015). (PMC4809531)

124. Luo, J.; Muratore, K.A.; Arriaga, E.A.; Ros, A. “Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device”, Anal. Chem. 88, 5920-5927 (2016). (NIHMSID: 844038)

125. Daniele, J.R.; Heydari, K.; Arriaga, E.A.*; Dillin, A. “Identification and Characterization of Mitochondrial Subtypes in Caenorhabditis elegans via Analysis of Individual Mitochondria by Flow Cytometry”, Anal. Chem. 88, 6309-6316 (2016). (NIHMSID: 844031)

126. Satori, C.P.; Ramezani, M.; Koopmeiners, J.S.; Meyer, A.F.; Rodriguez-Navarro, J.A.; Kuhns, M.M.; Taylor,T.H.; Haynes,C. Dalluge, J.J.; Arriaga, E.A. “Checkpoints for preliminary identification of small molecules found enriched in autophagosomes and activated mast cell secretions analyzed by comparative UPLC/MSe”, Analytical Methods, 9, 46 (2017). (NIHMSID: 844043)

127. Muratore, K.A.; Grundhofer, H.M.; Arriaga, E.A. “Capillary Electrophoresis with Laser-Induced Fluorescent Detection of Immunolabeled Individual Autophagy Organelles Isolated from Liver Tissue” Anal. Chem., 88, 11691–11698 (2016). (NIHMSID: 844035)

128. Palsuledesai, C.C.; Ochocki, J.D.; Kuhns, M.M.; Wang, Y.-C.; Warmka, J.K.; Chernick, D.S.; Wattenberg, E.V.; Li, L.; Arriaga, E.A. and Distefano, M.D. “Metabolic Labeling with an Alkyne-modified Isoprenoid Analog Facilitates Imaging and Quantification of the Prenylome in Cells”, ACS Chem. Biol. 11(10), 2820-2828 (2016). (PMC5074897)

Books and Book Chapters: 6 1. Dovichi, N. J.; Le, X. C.; Arriaga, E.; Pinto, D. "Capillary Electrophoresis and Laser-Induced Fluorescence

in Environmental Chemistry". In Environmental Measurement and Analysis; Koizumi, H., Ed.; Japan Science and Technology Corporation, 1997; pp 102-4.

2. Craig, D. B.; Arriaga, E. A; Wong, J. C .Y.; Lu, H.; Dovichi, N. J. "Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection", Tech. Protein Chem., VIII, 121-131 (1997).

3. Ahmadzadeh, H.; Arriaga, E. A. Chapter 8, “From Cells to Instrumental Analysis” in Sample Preparation for Hyphenated Analytical Techniques. Jack Rosenfeld, Editor, Blackwell Publishing: Oxford, UK and CRC Press: Boca Raton, FL, 2004, pp. 173-190.

4. Poe, B.G.; Arriaga, E.A. Chapter 20, “Subcellular Analysis by Capillary Electrophoresis,” in Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, 3rd edition, J.P. Landers, Editor, CRC Press: Boca Raton, FL, 2007, pp. 583 - 609.

5. Arriaga, E.A. “Single Cell Heterogeneity” Chapter 11, in Single Cell Analysis: Technologies and Applications. Dario Anselmetti, Editor, Wiley-VCH Verlag GmbH & Co: Weinheim, Germany, 2009, pp. 223-234.

6. Grundhofer, H.M.; Kuhns, M.M.; Arriaga, E.A.; High Resolution Interrogation of Biological Systems via Mass Cytometry. In Proteomics for Biological Discovery, 2nd Edition. Yates, J., Veenstra, T., Eds.; Wiley & Sons; In press.

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Editorials and Perspectives: 6 1. Sweedler, J.V., C.F.; Arriaga, E.A. “Single Cell Analysis,” Editorial, Anal. Bioanal. Chem. 387, 1-2

(2007).

2. Navratil, M.; Whiting, C.E.; Arriaga, E.A. “Microfluidic Devices for the Analysis of Single Cells: Leaving No Protein Uncounted”, Perspective, Science STKE 2007 (388), pe29 (2007).

3. Arriaga, E.A. “Within the Aging Muscle -Tools to Investigate the Role of Mitochondria in Aging”, Minnesota Physician, Invited article for special issue on senior care. April 2008, 22(1), 24-25.

4. Arriaga, E.A. König. “Analytical Tools for Cell Research”, Editorial, Anal. Bioanal. Chem. 397, 3161-3162 (2010).

5. Wolken, G.; Arriaga, E.A. “Isoelectric focusing of biological particles, AES Newsletter, 16 (4), 2 (2011).

6. Ochocki, J. D.; Igbavboa, U.; Wood, W. G.; Arriaga, E. A.; Wattenberg, E. V.; Distefano, M. D. “Evaluation of prenylated peptides for use in cellular imaging and biochemical analysis”, Meth. Mol. Biol. 2013, 1088, 213-223.

Patents: 1 1. Andersson, P.; Arriaga, E.; Ocvirk, G.; Harrison. D.J. “Microchip based enzymatic analysis,” Patented

Application: CA 2230653, Filed: Feb. 27, 1998 by the University of Alberta.

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Invited Conference Presentations

1. Arriaga, E. A.; Pinto, D.; Lee, L.; Zhang, Z.; Krylov, S.; Dovichi, N.J. "Exploring the Yoctomole Dimension: Proteins in Cells and Organelles", Federation of Analytical Chemistry and Spectroscopy Societies, FACSS 1998, Austin, Texas, October, 1998.

2. Arriaga, E. A.; Duffy, C.; Wolgamot, M.; Fuller, K.; Strack, A. "Mitochondrial Analysis by Capillary Electro-phoresis with Laser-Induced Fluorescence Detection", 46th International Conference on Analytical Sciences and Spectroscopy (46th ICASS), Winnipeg, Manitoba, Canada, August, 2000.

3. Arriaga, E. A.; Duffy, C.; Fuller, K.; Anderson, A.; Gunasekera, N.; Wolgamot, M. "Analysis of Individual Organelles by Capillary Electrophoresis with Laser-induced Fluorescence Detection", Pacifichem 2000, Honolulu, Hawaii, December 2000.

4. Arriaga, E. A.; Anderson, A.; Duffy, C. F.; Gunasekera, N., Fuller, K. "Analyzing Individual Organelles by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", 84th Canadian Society for Chemistry Conference (84th CSC), Montreal, Quebec, Canada, May 2001.

5. Arriaga, E. A. "Capillary Electrophoresis Analysis of Mitochondria, A Total Recount", Gordon Conference in Analytical Chemistry, New London, CT, June 2001.

6. Arriaga, E. A.; Anderson, A.; Gergen, Jamie, Ciriacks, C.; Abdalla, A. "Drug Metabolism and Distribution in Mammalian Single Cells", Federation of Analytical Chemistry and Spectroscopy Societies (FACSS'2001), Detroit, MI, October 2001.

7. Arriaga, E. A. "Developing Analytical Strategies for the Analysis of Subcellular Compartments", Leaders of the New Millennium Symposium, Federation of Analytical Chemistry and Spectroscopy Societies, (FACSS'2001), Detroit, MI, October 2001.

8. Arriaga, E. A.; Anderson, A.; Duffy, C.; Fuller, K.; Gunasekera, N.; McEathron, A.; Poe, B. "Electrophoretic Characterization of Subcellular Environments", 12th Frederick Conference on Capillary Electrophoresis, Frederick, MD, October 2001.

9. Arriaga, E. A. "Individual Liposomes, A Prelude to Organelle Analysis", Pittcon'2002, New Orleans, LA, March 2002.

10. Anderson, A.B.; Fuller, K.M.; Xiong, G.; Ciriacks, C.; Arriaga, E.A. "Analysis of Organelles after Electrophoretic Mobilization", 26th International Symposium on High Performance Liquid Phase Separations and Related Techniques, (HPLC 2002), Montreal, Canada, June 2002.

11. Arriaga, E. A.; Fuller, K.M. "Mitochondrial Diversity as Seen by Capillary Electrophoresis", 85th Canadian Society for Chemistry Conference (85th CSC), Vancouver, BC, Canada, June 2002.

12. Arriaga, E. A.; Fuller, K.M.; Xiong, G.; Gunasekera, N. "Capillary Electrophoretic Separation of Isolated Organelles", 44th Rocky Mountain Conference on Analytical Chemistry, Denver, CO, July-August 2002.

13. Fuller, K.M.; Gunasekera, N.; Poe, B.G.; Xiong, G.; Arriaga, E.A. "Exploring the Electrophoretic Behavior of Organelles", 224 National American Chemical Society Meeting, Boston, MA, August 2002.

14. Anderson, A.; Xiong, G.; Arriaga, E.A. "Towards Profiling Subcellular Drug Distributions", 13th Frederick Conference on Capillary Electrophoresis, Frederick, MD, October 2002.

15. Arriaga, E.A. "New Approaches in Individual Mitochondrial Analysis", Mitochondria Interest Group, NIH, (Video-Conference). November, 2002.

16. Fuller, K.M.; Gunasekera, N.; Johnson, R.; Xiong, G.; Arriaga, E.A. "Analysis of Isolated Organelles by Capillary Electrophoresis", Pittcon'2003, Orlando, FL, March 2003.

17. Arriaga, E.A. "Electrophoretic Behavior of Individual Organelles", 26th International Symposium on Capillary Chromatography & Electrophoresis, Las Vegas, NV, May 2003.

18. Arriaga, E.A. "Electrophoretic Behavior of Individual Organelles", Federation of Analytical Chemistry and Spectroscopy Societies, (FACSS'2003), Fort Lauderdale, FL, October 2003.

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19. Arriaga, E.A.; Whiting, C.; Olson, K.; Ahmadzadeh, H. "Using Microfluidic Devices For The Analysis Of Organelles", Federation of Analytical Chemistry and Spectroscopy Societies, (FACSS'2003), Fort Lauderdale, FL, October 2003.

20. Arriaga, E.A. “Subcellular Explorations by Capillary Electrophoresis”, Latin American Conference in Capillary Electrophoresis (LACE-2003), Mexico City, Mexico, December 2003.

21. Arriaga, E.A.; Chen, Y. “Quantum Dots for Detection of Individual Acidic Organelles”, Pittcon'2004, Chicago, IL, March 2004.

22. Arriaga, E.A.; Poe, B.; Walsh, R. “Fluorescently Guided Analysis of Organelles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry”, 227th National ACS Meeting, Anaheim, CA, April 2004.

23. Arriaga, E.A.; Johnson, R.D. “Analysis of Individual Organelles from Single Cells”. 36th Central Regional ACS Meeting, Indianapolis, IN, June 2004.

24. Arriaga, E.A.; Olson, K.; Whiting, C.; Duffy, C. “Exploring Mitochondrial Properties Using Microfluidic Devices”, Federation of Analytical Chemistry and Spectroscopy Societies, (FACSS'2004), Portland, OR, October 2004.

25. Arriaga, E.A.; Olson, K.; Whiting, C.; Duffy, C. "Microfluidic Devices for Organelle Analysis", 15th Frederick Conference on Capillary Electrophoresis/Proteomics, Frederick, MD, October 2004.

26. Johnson, R. D.; Xiong, G.; Ahmadzadeh, H.; Navratil, M.; Olson, K.; Arriaga, E. A. “Analysis of Individual Organelles from Single Cells”, Latin American Conference in Capillary Electrophoresis (LACE-2004), Toledo, Spain, November 2004.

27. Navratil, M.; Walsh, R.; Poe, B.G.; Arriaga, E.A. "Analysis of Organelles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Itraq Technology", Pittcon 2005, Orlando, Florida, March 2005.

28. Arriaga, E.; Xiong, G.; Eder, A.; Eddy, C. “Determining the Subcellular Fate of Anti-Cancer Agents,” 229th ACS National Meeting, ACS Nobel Laureate Signature Award Symposium: Spectroscopy at the Nanoscale, San Diego, CA, March 2005.

29. Arriaga, E.A. “En Busca de la Fuente de la Juventud: Historia de la Mitocondria,” Converciencia 2005, Hotel Camino Real, Guatemala City, Guatemala, July, 2005.

30. Arriaga, E.; Johnson, R.; Navratil, M.; Olson, K.; Chen, Y. “Heterogeneity in the Mitochondria of Single Cells Revealed by Individual Organelle Capillary Electrophoretic Analysis,” 2nd Münster Conference on Single Cell Analysis, Münster, Germany, December 2005.

31. Arriaga, E.; Li, D. “Capillary Electrophoretic Analysis of Individual Organelles Producing Reactive Oxygen Species”, Microscale Bioseparations & Methods for Systems Biology Conference, (MSB-2006), Amsterdam, The Netherlands, January 2006.

32. Arriaga, E.; Eder, A.R.; Chen, Y.; Whiting, C.E.; Poe, B.G. “Subcellular Perspectives on Cancer Drug Treatments Based on Individual Organelle Analyses,” 2006 Gordon Research Conference on Bioanalytical Sensors, Session: Single Cell Analysis, Ventura, CA, February – March, 2006.

33. Arriaga, E.A.; Ahmadzadeh, H.; Sanow, W.; Eddy, C.; Hong, J.; Cressman, E. “Hyphenating Solid Tissue Sampling with Capillary Electrophoresis”, Session for ACS Division of Analytical Chemistry Award for Young Investigators in Separation Science, Pittcon 2006, Orlando, FL, March 2006.

34. Arriaga, E.A. Invited Speaker, NSF Workshop on “Analytical Instrumentation for Biomeasurements” organized to determine future funding priorities, Session: “Multiscale Dynamics,” Tucson, AZ, April 20-21, 2006.

35. Arriaga, E.A. “Capillary Electrophoresis, a New Tool to Analyze the Individual Organelles of Single Cells”, First International Workshop on Single Cell Analyses, Uppsala University, Uppsala, Sweden, June 2006.

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36. Arriaga, E.A.; Poe, B.G.; Navratil, M. “Within Individual Mitochondria: A Genomic Analysis”, Symposium: Ralph N. Adams Award in Bioanalytical Chemistry, Pittcon 2007, Chicago, IL, February-March 2007.

37. Whiting, C.E.; Arriaga, E.A. “Individual Mitochondrial Analysis in Microfluidic Devices with Orthogonal Laser Induced Fluorescence Detection”, Canadian Society of Chemistry 2007 Conference, Winnipeg, MB, May 2007.

38. Arriaga, E.A. “–Estamos Oxidados–Mitos y Realidades de Oxidantes y Anti-Oxidantes”, Public Lecture. Converciencia 2007, Guatemala City, Guatemala, July, 2007.

39. Poe, B.G.; Andreyev, D.; Arriaga, E.A. “Fluorescence and Scattering Detection of Individual Particles by Capillary Electrophoresis and Flow Cytometry”, Federation of Analytical Chemistry and Spectroscopy Societies, (FACSS'2007), Memphis, TN, October 2007.

40. Xu, X.; Arriaga, E.A. “Using Capillary Electrophoresis to Monitor the Formation and Effects of Reactive Oxygen Species”, Latin American Conference in Capillary Electrophoresis (LACE-2007), Santiago, Chile, December 2007.

41. Arriaga, E.A. Xu, X.; Chen, Y.; Thompson, L. “Subcellular Analysis of Single Cells”, Symposium: Chemical Cytometry: The Chemical Analysis of Single Cells, Pittcon 2008, New Orleans, LA, March 2008.

42. Arriaga, E.A.; Kostal, V.; Xu, X.; Warg, E.; Whiting, C.E. “Subcellular Analysis Using Microfluidic Systems”, Symposium: Cellular Assays in Microfluidic Systems, Pittcon 2008, New Orleans, LA, March 2008.

43. Xu, X.; Sa, R.; Kostal, V.; Arriaga, E.A. “Separation of Oxidative Stress Markers from different Subcellular Environments”, Canadian Society of Chemistry 2008 Conference, Edmonton, AB, May 2008.

44. Arriaga, E.A.; Navratil, M.; Feng, J.; Xu, X.; Sa, R.; Kostal, V. “Investigating the Role of Giant Mitochondria in Aging”, Chemical Biology Interface Training Grant Symposium, University of Minnesota, Minneapolis, MN, May 2008.

45. Xu, X.; Navratil, M.; Thompson, L.; Arriaga, E.A. “Bioanalysis of Single Skeletal Muscle Fibers”, 23rd International Symposium on Microscale Bioseparations, (MSB 2009), Boston, MA, February 2009.

46. Arriaga, E.A.; Kostal, V.; Xu, X.; Wolken, G.; Navratil, M.; Thompson, L.V. “Giant Mitochondria Separations”, Symposium: Cellular and Sub-Cellular Separations, Pittcon 2009, Chicago, IL, March 2009.

47. Wolken, G.; Kostal, V.; Xiong, G.; Arriaga, E.A. "Electrophoretic separations of mitochondria: Evaluating isoelectric focusing". Symposium: Analytical Chemistry Award, 238th ACS National Meeting, Washington, DC, August 2009.

48. Arriaga, E.A.; Kostal, V.; Wolken, G. "Capillary Electrophoretic Analysis of Giant Mitochondria", Latin American Conference in Capillary Electrophoresis, LACE-2009, Seville, Spain, October 2009.

49. Arriaga, E.A.; Xin, X.; Sa, R.; Wang, Y.; Kostal, V. “Detecting Reactive Species in Single Cells”, Organized Contributed Session: Bioanalytical Approaches to Study Cellular Communication, Pittcon 2010, Orlando, FL, March 2010.

50. Arriaga, E.A.; “Single Muscle Fiber Analysis”, Technical Session: Micro-Nano Systems for Medical Applications 3, 2010 University of Minnesota Design of Medical Devices Conference, Minneapolis, MN, April 2010.

51. Arriaga, E.A.; Xin, X. “Quantitative Analysis of Reactive Oxygen Species in Single Cells”, 56th ICASS, Edmonton, AB, August 2010.

52. Arriaga, E.A.; Thompson, L.; Xu, X.; Donoghue, M. “Analysis of Reactive Oxygen Species in Single Cells and Tissues”, LACE-2010, Jurere Beach Village, Brazil.

53. Arriaga, E.A.; Wang, Y.; Cressman, E.K. "Direct Sampling from Explanted HumanLiver”, Symposium: On Site and In-vivo Instrumentation and Applications, Pacifichem 2010, Honolulu, HI, December 2010.

54. Arriaga E.A.; Holm, S.; Taylor, T. “Affinity Purification of Organelles to Investigate Biotransformations", Pacifichem 2010, Honolulu, HI, December 2010.

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55. Arriaga E.A.; Donoghue, M. “Subcellular Analysis of Reactive Oxygen Species using Capillary Electrophoretic Techniques", The 2nd SINO-USA Symposium on Separation Sciences, San Diego, CA, April 2011.

56. Arriaga E.A.; “Capillary Electrophoretic Analysis of Single Cells and Organelles", NIH Workshop: single Cell Studies in Aging Research, Bethesda, MD, May 2011.

57. Wolken, G.; Satori, C.; Taylor, T.; Kostal, V.; Arriaga, E. A “Capillary electrophoretic analysis of mitochondria and autophagosomes”, Federation of Analytical Chemistry and Spectroscopy Societies, (FACSS'2011), Reno, NV, October 2011.

58. Wolken, G.; Satori, C.; Taylor, T.; Doenges, J.; Rose, S.; Kostal, V.; Arriaga, E. A “Subcellular Complexity – an Electrophoretic Perspective”, Annual Meeting of the American Electrophoresis Society (2011 AlChE,) Minneapolis, MN, October 2011.

59. Wolken, G.*; Arriaga, E.A.“Characterization of membrane potential of individual mitochondria by capillary electrophoresis”, LACE-2011, Hollywood, FL, December 2011.

60. Donoghue, M.; Bernlohr, D.A.; Arriaga, E.A. “Simultaneous detection of reactive oxygen species using capillary electrophoresis”, Pittcon 2012, Orlando, FL, March 2012.

61. Doenges, J.; Wolken G.G.;Kostal, V.; Arriaga, E.A.* "A novel mobility correction method for individual organelle analysis by CE", Minnesota Chromatography Forum, Saint Paul, MN, May 2012.

62. Arriaga, E.A. "Quantitation of Reactive Oxygen Species and Oxidative Damage in Single Mammalian Cells", Single Cell Analysis Summit 2012, San Diego, CA, September 2012.

63. Arriaga, E.A. “Using capillary electrophoresis to investigate the role subcellular complexity in aging and disease”, Society of Hispanic Professional Engineers, Dallas – Fortworth, TX, November 2012.

64. Satori, C.P.; Henderson, M.M.; Taylor, T.H.; Shu, L.; Jagtap, P.D.; Onsongo, I.G.; Chilton, J.R.; Arriaga, E.A. “Immunoenrichment of organelles for electrophoretic and proteomic analysis”, LACE-2012, Buenos Aires, Argentina, December 2012.

65. Wolken, G.G; Fossen, B.J.; Noh, A.; Arriaga, E.A. “Defining organelle surface compositions through isoelectric focusing of individual organelles”, Pittcon-2013, Philadelphia, PA, March 2013.

66. Satori, C.P.; Arriaga, E.A. "Teasing Apart Age-Related Sub-Cellular Complexity", AGE 2013: Aging, Prevention, Reversal, Slowing, Baltimore, MD, May-June 2013.

67. Arriaga, E. A.; Satori, C. “Electrophoretic analysis of individual autophagosomes”, SciX 2013, Milwaukee, WI, September-October 2013.

68. Wolken, G.G; Daniele, J.R.; Dillin, A.; Arriaga, E.A. “The biological membrane potential of individual mitochondria after isolation”, Eastern Analytical Symposium and Exposition, Somerset, NJ, November 2013.

69. Wolken, G.G; Arriaga, E.A. “Measurement of Individual Mitochondrial Membrane Potential by Capillary Electrophoresis”, Pittcon 2014, Chicago, IL, March 2014.

70. Arriaga, E.A., Daniel, J. “A Tale of Two Cores: Single Cell Genomics and Proteomics”, The Southeastern Association of Shared Resources (SEASR) and the Midwest Association of Core Directors (MWACD) Conference, Nashville, TN, October 2014.

71. Arriaga, E.A., “Approaches to Describe Sub-cellular Specific Prodrug-Transformations”, American Association of Pharmaceutical Scientists (AAPS), San Diego, CA, November 2014.

72. Arriaga, E.A., “Taking Advantage of Technical Conferences”, Society of Hispanic Professional Engineers (SHPE) Regional Leadership Development Conference (RLDC), Minneapolis, MN, April 2015.

73. Muratore, K.; Manion-Fisher, D.; Shankarappa, M.; Lanza, I.; Nair, S; Arriaga, E.A., “Exploring Subcellular Complexity In Tissues By Capillary Electrophoretic Analysis Of Individual Organelles”, 60th International Conference on Analytical Sciences and Spectroscopy (60th ICASS), Halifax, NS, Canada, May 2015.

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74. Arriaga, E.A. Kuhns (Henderson), M.; Grundhofer, H.; Muratore, K. “The Quest for Single Cell Proteomics - La busqueda de técnicas proteómicas para células individuales”, 8avo Congreso Argentino de Química Analítica, La Plata, Argentina, November 2015.

75. Arriaga, E.A. Kuhns (Henderson), M.; Grundhofer, H.; Parsons, L. “The Quest for Single Cell Proteomics”, 21st Latin-American Symposium on Biotechnology, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology (LACE 2015), Cartagena, Colombia, December 2015.

Invited Extramural Seminars

1. *Arriaga, E. A. "Análisis de biomoléculas usando electroforesis capilar con fluorescencia inducida por laser", Workshop. Primer Taller de Química Analítica de la Universidad del Valle, Guatemala City, Guatemala, 1995.

2. *Arriaga, E. A.; "Shrinking Analytical Chemistry for Single Cell Analysis", Department of Chemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, January, 1998.

3. Arriaga, E. A. "Exploring the Yoctomole Dimension: Proteins in Cells and Organelles", Macalister College, St. Paul, MN, November, 1998.

4. Arriaga, E. A. "Analisis de Células y Organelos", Universidad del Valle de Guatemala, April, 2000.

5. Arriaga, E. A. "Organelle Analysis by Capillary Electrophoresis with Laser Induced Fluorescence Detection", Saint Thomas University, Saint Paul, MN, October, 1999.

6. Arriaga, E. A. "Single cell and Organelle Analysis by Capillary Electrophoresis with Laser Induced Fluorescence Detection", Ripon College, Ripon, WI, November, 1999.

7. Arriaga, E. A. "Miniaturizing Analytical Chemistry: Analysis of Cells and Organelles", North Dakota State University, Fargo, ND, March, 2000.

8. Arriaga, E. A. "Mitochondrial Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", University of Wisconsin, Oshkosh, Wisconsin, September, 2000.

9. Arriaga, E. A. "Looking for Answers inside the Cell: Organelle Analysis", Dalhousie University, Halifax, Nova Scotia, Canada, November 2000.

10. Arriaga, E. A. "Organelle Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", University of New Brunswick, Fredericton, New Brunswick, Canada, November 2000.

11. Arriaga, E. A. "Looking for Answers inside the Cell: Organelle Analysis", Acadia University, Kentville, Nova Scotia, Canada, November 2000.

12. Arriaga, E. A. "Organelle Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection", Saint Mary's University, Halifax, Nova Scotia, Canada, November 2000.

13. Arriaga, E. A. "Looking for Answers inside the Cell: Organelle Analysis", University of Manitoba, Winnipeg, Manitoba, Canada, January 2001.

14. Arriaga, E. A. "Looking for Answers inside the Cell: Organelle Analysis", University of Winnipeg, Winnipeg, Manitoba, Canada, January 2001.

15. Arriaga, E. A. "Looking for Answers inside the Cell: Organelle Analysis", University of Illinois, Urbana-Champaign, IL, January 2001.

16. Arriaga, E.A. "New Analytical Strategies for Subcellular Compartments", Chemical Biology Colloquium, University of Minnesota, October, 2001.

17. Arriaga, E. A. "Identification of Subcellular Components by Capillary Electrophoresis", University of Wisconsin, Eau-Claire, WI, November 2001.

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18. Arriaga, E.A. "Use of Capillary Electrophoresis for the Analysis of Single Cells", Gustavus Adolphus College, St. Peter, MN, November, 2001.

19. Arriaga, E.A. "Electrophoretic Characterization of Subcellular Environments", Pennsylvania State University, College Station, PA, February, 2002.

20. Arriaga, E. A. “Microfabrication, Analysis and Manipulation of Cellular Organelles,” Medtronic Forum, Minneapolis, MN, February, 2002.

21. Arriaga, E.A. "Electrophoretic Characterization of Subcellular Environments", University of Arizona, Tucson, AZ, April, 2002.

22. Arriaga, E.A. "Nuevas estrategias bioanalíticas para investigar el interior de la célula", Universidad del Valle de Guatemala, Guatemala City, Guatemala, May 2002.

23. Arriaga, E.A. "Studying Subcellular Diversity: Electrophoretic Characterization of Individual Organelles", San Francisco State University, San Francisco, CA, May, 2002.

24. Arriaga, E.A. "How many Cardiolipin Molecules are there in an Individual Mitochondrion", Molecular Biophysics, Biotechnology, and Chemistry-Biology Interface Training Grant Symposium, University of Minnesota, St. Paul, MN, May 2002.

25. Arriaga, E.A. "Emerging Strategies for Subcellular Analysis", University of Arizona, Tucson, AZ, September, 2002.

26. Arriaga, E.A. "Analysis of Isolated Organelles by Capillary Electrophoresis", York University, York, Ontario, Canada, October 2002.

27. Arriaga, E.A. "Expanding the Scope of Capillary Electrophoresis to the Analysis of Particles and Organelles", University of Toronto, Toronto, Ontario, Canada, October 2002.

28. Arriaga, E.A. "Analysis of Isolated Organelles by Capillary Electrophoresis", University of Western Ontario, London, Ontario, Canada, October 2002.

29. Arriaga, E.A. "Analysis of Isolated Organelles by Capillary Electrophoresis", Saint Olaf College, Northfield, MN, November 2002.

30. Arriaga, E.A. "New Approaches in Individual Mitochondrial Analysis", Mitochondria Interest Group, NIH, (Video-Conference). November, 2002.

31. Arriaga, E.A. "Analysis of the Subcellular Distribution and Metabolism of Anti-Cancer Drugs", University of Pittsburgh, Pittsburgh, PA, November, 2002.

32. Arriaga, E.A. "Emerging Strategies for Subcellular Analysis", Iowa State University, Ames, IA, November, 2002.

33. Arriaga, E.A. "Analysis of Isolated Organelles by Capillary Electrophoresis", Saint Cloud State University, Saint Cloud, MN, November 2002.

34. Arriaga, E.A. "Emerging Strategies for Subcellular Analysis", University of Alberta, Edmonton, Alberta, Canada, January, 2003.

35. Arriaga, E.A. "Looking for Answers Inside the Cell", Truman State University, Kirksville, MO, March, 2003.

36. Arriaga, E.A. "Emerging Strategies for Subcellular Analysis", University of Iowa, Iowa City, IA, April, 2003.

37. Arriaga, E.A. "Analysis of Mitochondria: Uncovering the Secrets of Aging", California State University, Los Angeles, CA, April, 2003.

38. Arriaga, E.A. "Emerging Strategies for Subcellular Analysis", Dublin City University, Dublin, Ireland, September, 2003.

39. Arriaga, E.A. “Investigating the Electrophoretic Mobilities of Individual Organelles”, Georgia State University, Atlanta, GA, October, 2003.

40. Arriaga, E.A. “Analyzing Organelles from Single Cells”, Pomona College, Claremont, CA, October, 2003.

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41. Arriaga, E.A. “Subcellular Explorations by Capillary Electrophoresis”, Southern Illinois University, Carbondale, IL, November, 2003.

42. Arriaga, E.A. “Emerging Strategies for Subcellular Analysis”, University of Texas, Austin, TX, November, 2003.

43. Arriaga, E.A. “Analysis of Individual Organelles Released from Single Cells”, Vanderbilt University, Nashville, TN, October, 2004.

44. Arriaga, E.A. "Measuring Properties of Organelles Sampled from Single Cells and Muscle Fibers", Dept. of Chemistry, University of Washington, Seattle, WA, March, 2005.

45. Arriaga, E.A. "Measuring Properties of Organelles Sampled from Single Cells and Muscle Fibers", Wake Forest University, Winston-Salem, NC, March, 2005.

46. Arriaga, E.A. “En Busca de la Fuente de la Juventud: Historia de la Mitocondria”, Facultad de Medicina, Universidad San Carlos de Guatemala, Guatemala City, Guatemala, July, 2005.

47. Arriaga, E.A. “New Approaches to Elucidate the Role of Acidic Organelles in Drug-Resistance”, Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, September, 2005.

48. Arriaga, E.A. “Pushing the Limits of Bioanalysis: Single Cells and Individual Organelles”, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic, May 2006.

49. Arriaga, E.A. “Measuring the Properties of Individual Biological Particles by Capillary Electrophoresis”, Institute of Hydrochemistry and Chemical Balneology, Technical University of Munich, Munich, Germany, June 2006.

50. Arriaga, E.A. “Mining Single Cells: Analysis of Individual Mitochondria from Single Cells”, Institute of Human Genetics, GSF – National Research Center for Environment and Health and TUM Klinikum Rechts der Isar, Munich-Neuherberg, Germany, October 2006.

51. Arriaga, E.A. “Analyzing Organelles from Single Mammalian Cells”, Institute for Analytical Chemistry, University of Vienna, Vienna, Austria, October 2006.

52. Arriaga, E.A. “Interrogating Individual Organelles and Other Biological Particles”, Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada, November 2006.

53. Arriaga, E.A. “Analysis of Individual Organelles Taken from Single Cells”, Department of Chemistry, University of California, Riverside, CA, March 2007.

54. Arriaga, E.A. “Within Individual Mitochondria, a Genomic Analysis”, Department of Chemistry, University of Alberta, Edmonton, AB, May 2007.

55. Arriaga, E.A. “Estamos Oxidados – Mitos y Realidades de Oxidantes y Anti-Oxidantes”, Universidad Galileo, Guatemala City, Guatemala, July, 2007.

56. Arriaga, E.A.”Gigantes en la Célula: Técnicas para Investigar la Mitocondria”, Universidad Francisco Marroquín, Guatemala City, Guatemala, July, 2007.

57. Arriaga. E.A. “Bioanalytical Strategies to Investigate the Roles of Giant Mitochondria in Aging”, University of Michigan, Ann Arbor, MI, October 2008.

58. Arriaga. E.A. “Analysis of Mitochondrial Dysfunction in Aging”, MIchigan State University, Lansing, MI, April 2009.

59. Arriaga, E.A. “Analysis of Mitochondrial Dysfunction in Aging”, Department of Chemistry, University of Winnipeg, July 2009.

60. Arriaga, E.A. "Direct sampling of human hepatocarcinomas for capillary electrophoretic analysis of anti-cancer drugs", University of Manitoba, July 2009.

61. Arriaga, E.A. “Electrophoretic Separations of Mitochondria”, Indiana University Purdue University Indianapolis, Indianapolis, IN, September 2009.

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62. Arriaga, E.A. “Unraveling biological complexity – Analysis of tissue, cells, and organelles”, Texas Tech University, Lubbock, TX, November 2009.

63. Arriaga, E.A. “Unraveling biological complexity – Analysis of tissue, cells, and organelles”, University of South Dakota, Sioux Falls, SD, December 2009.

64. Arriaga, E.A. “Unraveling biological complexity – Microanalysis of tissues, cells and organelles”, University of Kentucky, Lexington, KY, January 2010.

65. Arriaga, E.A. “Analysis of reactive oxygen species in biological systems using molecular probes and capillary electrophoresis”, Gustavus Adolphus College, St. Peter, MN, February 2010.

66. Arriaga, E.A. “Investigating the role of mitrochondria in aging”, University of Ottawa, Ottawa, ON, Canada, April 2010.

67. Arriaga, E.A. “Bioanalytical strategies to explore the role of reactive oxygen species in skeletal muscle mitochondria”, University of Toronto, Toronto, ON, Canada, September 2010.

68. Arriaga, E.A. “Subcellular Analytical Strategies”, University of Toledo, Toledo, OH, January 2011.

69. Arriaga, E.A. “Subcellular Analytical Strategies”, Georgia Institute of Technology, Atlanta, GA, February 2011.

70. Arriaga, E.A. “Subcellular Analysis of Autophagy Deficient Cells Using Capillary Electrophoresis, Proteomics, and Lipidomics”, Oslo universitetssykehus – Radiumhospitalet, Oslo, Norway, June 2011.

71. Arriaga, E.A. “Bioanalytical Chemistry at the Subcellular Level - Investigating the Role of Mitochondria in Aging”, University of North Dakota, Grand Forks, MN, February 2012.

72. Arriaga, E.A. “Using capillary electrophoresis to investigate the role subcellular complexity in aging and disease”, California State, Los Angeles, Los Angeles, CA, March 2013.

73. Arriaga, E.A. “Using capillary electrophoresis and proteomics to investigate the role subcellular complexity in aging and disease”, Saint Louis University, Saint Louis, MO, April 2013.

74. Arriaga, E.A. “Analyzing Self-Eating”, University of California - Riverside, Riverside, CA, December 2013.

75. Arriaga, E.A. “Multifaceted Analysis of Self-Eating”, University of Notre Dame, South Bend, IN, October 2014.

76. Arriaga, E.A. “Multifaceted Analysis of Self-Eating: A Mass Cytometry Perspective”, R&D Systems, Minneapolis, MN, October 2014.

77. Arriaga, E.A. “Teasing apart cellular self-eating (Autophagy): From individual organelle analysis to single cell proteomics”, Dalhousie University, Halifax, NS, Canada, May 2015.

78. Arriaga, E.A. “The Quest for Single Cell Proteomics”, Biotechne, Minneapolis, MN, June 2015.

79. Arriaga, E.A. “The Quest for Single Cell Proteomics”, Michigan State University, Lansing, MI, October 2016.

80. Arriaga, E.A “The Quest for Single Cell Proteomics - La busqueda de técnicas proteómicas para células individuales”, Fluidigm, Webinar, November 2015.

Invited Intramural Seminars

81. Arriaga, E. A. "CE-LIF for Single Cell Analysis", Biological Process Technology Institute, University of Minnesota, St. Paul, MN, November, 1998.

82. Arriaga, E. A. "Organelle Separations by Capillary Electrophoresis with Laser Induced Fluorescence Detection", University of Minnesota–Duluth, Duluth, MN, March, 1999.

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83. Arriaga, E. A. "Single cell and Organelle Analysis by Capillary Electrophoresis with Laser Induced Fluorescence Detection", University of Minnesota-Morris, Morris, MN, October, 1999.

84. Arriaga, E.A. "Diagnosing Heteroplasmy at an Early Stage: Individual Mitochondrial Measurements". Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, October 2002.

85. Arriaga, E.A. “Analysis of Individual Organelles from Single Cells”, Dept. of Biochem. Mol. Biol. & Biophysics, University of Minnesota, November, 2004.

86. Arriaga, E.A. “Sensitive Analysis of the Subcellular Distributions and Effects of Anthracyclines”, Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN, December 2006.

87. Arriaga, E.A. “Quantitating Proteomic Changes after Mild Oxidative Stress”, Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota—Twin Cities, July 2007.

88. Arriaga, E.A. “Using Capillary Electrophoresis to Investigate Reactive Oxygen Species Production and Damage”, Integrated Biosciences Program, University of Minnesota–Duluth, Duluth, MN, November 2007.

89. Arriaga, E.A. “Using Capillary Electrophoresis to Monitor Doxorubicin-Leucin Transformation and Metabolism”, University of Minnesota Cancer Center, University of Minnesota–Twin Cities, Minneapolis, MN, January 2008.

90. Arriaga, E.A. “What is the Role of Giant Mitochondria in Aging”, 2007-2008 Neurology Grand Round Series, Department of Neurology, University of Minnesota–Twin Cities, June 2008.

91. Arriaga, E.A. “Uncovering the Roles of Giant Mitochondria in Aging”, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota–Twin Cities, September 2008.

92. Arriaga, E.A. “Tools to Investigate Mitochondrial Dysfunction in Aging”, 2009 Scientists in Aging Research Retreat, University of Minnesota–Twin Cities, February 2009.

93. Arriaga, E.A. “Analysis of Mitochondrial Dysfunction in Aging”, Department of Chemistry, University of Minnesota–Twin Cities, March 2009.

94. Arriaga, E.A. “Unraveling biological complexity – Analysis of tissue, cells, and organelles”, Department of Chemistry, University of Minnesota-Twin Cities, October 2009.

95. Arriaga, E.A. “Does age affect the in vitro metabolism of doxorubicin?”, Cancer Center, University of Minnesota-Twin Cities, February 2011.

96. Arriaga, E.A. “Affinity Purification of Organelles”, Cancer Center, University of Minnesota-Twin Cities, March 2011.

97. Arriaga, E.A. “Why pushing the limits? Single cell to individual organelle analysis?” MD/Ph.D Program, University of Minnesota-Twin Cities, April 2011.

98. Arriaga, E.A. “Novel bioanalytical strategies to investigate mitochondrial roles in age- and disease-related systems”, Institute for Translational Neuroscience, University of Minnesota-Twin Cities, November 2011.

99. Arriaga, E.A. “Bioanalytical strategies to investigate the role of autophagy in aging”, Department of Chemistry, University of Minnesota-Twin Cities, April 2014.

100. Arriaga, E.A. “Emerging Technologies for Individual Cell or Organelle Analysis”, Center for Orphan Drug Research, University of Minnesota-Twin Cities, April 2014.

101. Arriaga, E.A. “Emerging Technologies for Individual Cell or Organelle Analysis”, Carcinogenesis and Chemoprention Program Research in Progress Meeting, University of Minnesota-Twin Cities, May 2014.

102. Arriaga, E.A. “Mass Cytometry for Multi Parametric Analysis of Single Cells at the University of Minnesota”, Single Cell Biology User Group – University of Minnesota Genomics Center, University of Minnesota-Twin Cities, June 2014.

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103. Arriaga, E.A. “Mass Cytometry for Multi Parametric Analysis of Single Cells at the University of Minnesota”, University of Minnesota - Center for Immunology, University of Minnesota-Twin Cities, October 2014.

104. Arriaga, E.A. “Multifaceted Analysis of Autophagy: individual organelles, proteomics and mass cytometry”, University of Minnesota – College of Veterinary Medicine, Saint Paul, MN, February 2015.

105. Arriaga, E.A. “The Quest for Single Cell Proteomics”, University of Minnesota – Integrative Biology and Physiology, November 2015.

Contributed Conference Oral Presentations (92) Contributed Conference Poster Presentations (90)

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Research Support

Title/Agency Period Institute of Technology/Department of Chemistry, University of Minnesota Start-up Funds

1998 -

"Chemistry Department Phosphoimager", Grant-in-Aid, University of Minnesota, Joint Grant, Co-PI: Dr. K. Musier-Forsyth

6/15/98

"Bioanalysis of Subcellular Compartments (Organelles) Using Electrophoresis in Capillaries and in Microfabricated Devices"/Grant-in-Aid, University of Minnesota

1/1/99-01/15/00

Microtechnology Laboratory, University of Minnesota Research Incentive 5/13/99-12/31/00

"A Novel Instrument for DNA-Analysis in Individual Mitochondria to Determine Heteroplasmy within Single Cells"/Research Corporation, RIO477

5/15/00-12/31/01

"Novel Microfluidic Devices for Organelle Analysis"/ Grant-in-Aid, University of Minnesota

6/15/00 - 12/15/01

"Single Mitochondrium Analysis, a Tool for Defining Heteroplasmy and Its Role in Aging"/ NIH, (NIA) R03 AG18099

7/1/00-06/31/01

"Molecular Nanoscience Alliance for Interdisciplinary Studies and Activities", University of Minnesota, PI: Dr. Richard A. Kiehl

9/1/00-8/31/02

"Determining Subcellular Drug Distributions"/ NIH (NIGMS) R01 GM61969A1 4/01/01 - 3/31/06

"Defining Heteroplasmy at the Single Mitochondrion Level", NIH, R01-AG20866 4/15/02 – 3/31/07

"Determining Subcellular Drug Distributions"/ NIH (NIGMS) R01 GM61969-01A1, Supplementary Equipment Request

9/01/03 – 5/31/04

“Introduction of Capillary Electrophoresis to the Undergraduate Laboratory” / Camille and Henry Dreyfus Special Grant Program in the Chemical Sciences, PIs: Michael Bowser and Edgar Arriaga

05/01/04 – 12/31/04

"Heteroplasmy at the Single Mitochondrion Level" / NIH, Independent Scientist Career Award, 1K02-AG21453

4/01/03 - 3/31/08

“Aging of the Subcellular Muscle Proteome,” / NIH 1R21-AG025371, PI: Dr. Edgar Arriaga (3 faculty)

09/30/04 – 07/31/07

"Giant Mitochondria in Aging"/ Grant-in-Aid, University of Minnesota 2/1/07 - 6/30/07

“2007-2008 Fesler-Lampert Chair in Aging Studies”, Center on Aging, University of Minnesota

7/1/07-6/30/08

“Giant Mitochondria in Aging”, NIH 2-R01-AG20866. Competitive Renewal. 04/01/07 – 03/31/12

NIH R01HL089307-01A2, PI: Edward O. McFalls, (Arriaga, co-Investigator), 4 faculty, “Mitochondrial Protection within Chronically Ischemic Myocardium”.

07/01/09 – 06/30/12

NIH 2-R01-AG20866S1, Minority Undergraduate Supplement (PA-08-190), “Giant Mitochondria in Aging”.

07/01/09-10/15/09

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NIH 2-R01-AG20866S2, Minority Post-Bac Supplement (PA-08-190), “Giant Mitochondria in Aging”.

10/16/09-10/15/10

3P30DK050456-14S1, PI: Allen Levine, 5 projects, (Arriaga, Project 12992 PI, and Project 12993 co-Investigator).

07/25/09 - 05/31/11

NIH 1R01 DK084669-01A1,PI: David A. Bernlohr, (Arriaga, co-Investigator), 3 faculty, “Mitochondrial Dysfunction and Adipose Insulin Resistance”,

08/01/10 – 07/31/15

NIH R01AG020866, PI: Edgar Arriaga, Minority Post-Bac Supplement (PA-08-190). Trainee Candidate: Katherine Felder

03/15/11 – 03/14/12

OVPR, UMN. PI: Edgar Arriaga, Prosolia Omni Spray Two-Dimensional Desorption Electrospray Ionization (DESI) Mass Spectrometry Source.

1/1/12 - 6/30/13

AHC, PI: D. Ferrington, I. Stepanov, E. Arriaga, Characterizing Mitochondrial DNA Damage in Age-Related Macular Degeneration.

1/1/12 - 12/30/13

NIH R01-AG20866 PI: Edgar Arriaga, Quantitating mitophagy in skeletal muscle models.

04/01/12 – 03/31/17

NIH 3-R01AG020866-S1, PI: Edgar Arriaga, Minority Post-Bac Supplement (PA-08-190). Trainee Candidate: Katherine Felder

04/01/12 – 08/31/12

NIH 3-R01AG020866-10A1S2, PI: Edgar Arriaga, Quantitating mitophagy in skeletal muscle models – Instrumentation.

04/01/12 – 03/31/13

NIH 3-R01AG020866-12S1, PI: Edgar Arriaga, Quantitating mitophagy in skeletal muscle models – Diversity Supplement. Trainee Candidate: Deirdre Manion-Fischer

06/01/14 – 03/31/16

OVPR, CSE, Medical School, Chemistry, University of Minnesota, RISS, PI: Edgar Arriaga, Bringing mass cytometry to the University of Minnesota

07/01/13 – 06/30/15

OVPR, Medical School, Chemistry, Stem Cell Institute, and Center for Immunology, University of Minnesota, RIRP, PI: Dan Kaufman, (Arriaga, Co-PI), Introducing Researchers at University of Minnesota to Mass Cytometry

07/01/14 – 06/30/15

Minnesota Partnership for Biotechnology and Medical Genomics, MNP IF#14.06. PI: Edgar Arriaga (James Kirkland, co-PI), Mass Cytometry Infrastructure for Fundamental and Translational Research in Minnesota

07/01/14 – 06/30/16

NIH, 2 R01 AG020866-14, PI: Edgar Arriaga, Interplay between lipophagy and mitophagy in skeletal muscle aging

04/01/16 – 03/31/21

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Pending Support

Title/Agency Period None

Current Group Members Postdoctoral and Research Associates Graduate Students Michelle Henderson, December 2009 – on leave Elyse Krautkramer (co-advisor M. Distefano), November 2011 - Erik Tyrell, November 2011 - Marzieh Ramezani, November 2012 - Heather Grundhofer, November 2013 - Katherine Mutatore, January 2013 – Undergraduate Students Daniel Bolton, January 2016 - Erica Daniels, January 2016 - Daniel Esping, May 2014 - Zach Paull, January 2016 - Claire Porter, September 2015 – Tyler Wilson, January 2016 -

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Past Group Members Ph.D. Students (15) Adrian Anderson, January 1999 - August 2003 Current position: Associate Director-QC Chemistry, Gilead Sciences, Oceanside, California Thesis title: Strategies for the Elucidation of Doxorubicin Metabolism by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. Dmitry Andreyev, June 2001 – September 2007 Current position: Head of Analytics, Bind Therapeutics, Moscow, Russia Thesis title: Development of Models and Analytical Approaches for Individual Mitochondria. Yun Chen, December 2002 – December 2006 Current position: Associate Professor, Dept. Pharmacology, Nanjing Medical University, China Thesis title: Determination of Doxorubicin Content in Individual Acidic Organelles by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. Angie Eder, December 2002 – May 2006 Current position: Principal Chemist, 3M, Maplewood, MN. Thesis title: The Effect of Drug Delivery Systems on the Subcellular Metabolism of Doxorubicin And Cellular Response. Juan Feng (Biomedical Engineering), October 2004 – November 2007 Current position: Engineering Program Director, Bossier Parish Community College (BPCC), Shreveport, LA. Thesis title: Feng, J. Monitoring Age-associated Carbonylation in Rat Skeletal Muscle. Kathryn Fuller, January 1999 - July 2003 Current position: Forensic Scientist, MN Bureau of Criminal Apprehension, St. Paul, MN. Thesis title: Analysis of Individual Mitochondria and Acidic Organelles by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. Nilhan Gunasekera (Co.Advisor: KMF), January 1999 – August 2003 Current position: Teacher, Madison Area Technical College, Madison, WI. Thesis title: Investigations into the Use of Capillary Electrophoresis for the Analysis of Nuclear Preparations and the Nuclear Localization of Fusion Proteins at the Single Cell Level. Joseph Katzenmeyer, November 2004 – September 2010 Current position: Analytical Director, Land O’Lakes, Arden Hills, MN. Thesis title: HPLC-LIF-MS methods to monitor the metabolic profile of doxorubicin and its alteration with age. Danni Li Meany, December 2002- March 2007 Current position: Assistant Professor, University of Minnesota, Minneapolis, MN. Thesis title: Mitochondrial Superoxide Production and Its Oxidative Damage to Proteins, Ph.D., University of Minnesota, Minneapolis, 2007. Bobby G. Poe, June 2001 – April 2007

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Current position: Research Scientist, Life-Science Innovations, Willmar, MN. Thesis title: Analysis of Mitochondrial DNA in Individual Mitochondrial Particles. Chad P. Satori, October 2007 – April 2012 Current position: Senior Scientist, Medtronic, Minneapolis, MN. Thesis title: Advancing Organelle Analysis: Development Of Methods For The Enrichment Of Endocytic Organelles And For The Determination Of Endocytic And Autophagy Organelle Properties. Yaohua Wang, October 2004 – September 2010 Current position: Senior Chemist, Henkel, Madison Heights, Detroit, MI. Thesis title: Novel capillary electrophoretic strategies for investigating subcellular metabolism in vivo and in vitro. Chris Whiting, February 2002 – October 2007 Current position: Research Scientist, Dayton Research Institute, Dayton, OH. Thesis title: Statistical Analysis of Electrophoretic Mobility Distributions of Individual Mitochondria. Gregory Wolken, December 2007 – October 2013 Current position: Lignatech, WI. Thesis title: Bioanalytical Techniques For The Analysis Of Mitochondrial Heterogeneity In Aging Models. Xin Xu, October 2004 – June 2010 Current position: Forensic Scientist, Columbus Crime Laboratory, Columbus, MS. Thesis title: Monitoring Superoxide Levels in Skeletal Muscle and Cell Models by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. M. S. Students (Plan B, no thesis) (8) Scott Brown, January 2004 – August 2005 – August 2007 Current position: Graduate Student, School of Kinesiology, University of Minnesota Ryan Clark, December 2013 – March 2015 Current position: Unknown Jennifer (Sarafolean) Gonerka , January 2004 – October 2005 Current position: Research Scientists Boston Scientific Corporation, Forest Lake, MN C.J. Kurth, April 2001 – May 2003 Current position: Vice President of Engineering, NanoH2O, El Segundo, CA Shu Luan, November 2011 – April 2013 Current position: Unknown, China Deirdre Manion-Fischer, January 2010 – April 2016 Current position: Free lance writer Scott Rose (Masters, CBS), May 2009 - April 2012 Current position: Quality Assurance Medical Engineer, Medivir, Plymouth, MN

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Rongxiao Sa, October 2004 – May 2010 Current position: High School Teacher, Shaoxing, Zhe Jiang, China Scott Rose (Masters, CBS), May 2009 - April 2012 Current position: Quality Assurance Medical Engineer, Medivir, Plymouth, MN William Sanow, November 2004 – September 2007 Current position: Unknown Thane Taylor, (co-advisor M. Bowser), November 2008 – August 2014 Current position: Unknown Lucas Ulmer (Masters BME), May 2012 – August 2013 Current position: Ph.D. Program at the University of Utah Megan Wolgamot, November 1998 – January 2000 Current position: Principal Chemist, Ecolab Inc, Eagan, MN Postdoctoral Research Associates (9) Hossein Ahmadzadeh, September 2002 – August 2005

Current Position: Professor, Ferdowsi University of Mashhad, Iran

Dr. Margaret Donoghue, Ph.D., Indiana University, April 2010 – October 2012 Current Position: Instructor, Appalachian State University, NC

Ciarán F. Duffy, April 2000 – March 2001 Current Position: National Contact Point - Health, Enterprise Ireland, Dublin, Ireland Chris Eddy, May 2004 – March 2006 Current Position: Researcher, Seagate, Shakopee, MN Vratislav Kostal, February 2006 – June 2011 Current Position: Applications Specialist, Tescan, Czech Republic Marian Navratil, April 2004 – July 2008 Current Position: General Manager, R&D, Beckman Coulter, Ramsey, MN Hongjun Shu, Jan 1999 – April 1999 Current Position: Unknown Richard Walsh, June 2002 – July 2004 Current Position: Principal Chemist, Ecolab Inc., Eagan, MN Guohua Xiong, November 2001 – December 2004 Current Position: Senior Scientist, Analytical Bio-Chemistry Laboratories Inc., Columbia, MO Visiting Professors & Scholars (5)

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David Samuels, Dept. of Mathematics, University of Newcastle, August-December 2001 Scott Hartsel, University of Wisconsin–Eau Claire, September 2002 – August 2003 David Lewis, University of Wisconsin–Eau Claire, September 2004 – May 2005 Gary Mabbott, University of St. Thomas, St. Paul, MN, Summer 2003, June 2005 – May 2006. Xing-Zheng Wu, Fukui University, Japan, September 2008 - December 2008 Olga Regueiro – Vilar (Instituto de Química Orgánica General, CSIC, Spain), April – July 2013; Tomas Vaclavek (Institute of the Academy of Sciences, Czech Republic), May – June 2014; May -June 2015 Other Graduate Students Supervised (9): Amir Abdalla (MSERC); Ciarán Duffy (Visiting scholar from Dublin City University) Rajat Dua (Comp. and Elec. Eng.), August 2001 – May 2003; Nick Ergang (Internship); Aimee Erickson (Internship); Rahul Gupta (Comp. and Elec. Eng.), June 2003 – August 2003; Bill Sanow, (Analytical), November 2004 – September 2006; Lifeng Wang (Statistics), June 2003 – August 2003. Post-Baccalaureates Directed (2): Beryl Ombaso, September 2009 – August 2010, Katherine Felder, March 2011 - August 2012 Undergraduate Research Assistants who attended the University of Minnesota (94): Abdimalik Ahmud, Arielle Anderson, Frank J. Beissel, Nathan Bjerke, Kiara Brancel, Bernard Brooks, Marylen Challander, Eric Chapman, Mark Chapman, Michael Castle, Jennifer Chen, David Chia, Michael Childe, Jake Choi, Connie Chung, Maya Cutkosky, Aryel Diker, Jack Doenges, Peter Falvey, Katherine Felder, Benjamin Fossen, Craig Foster, Sheik Gafoor, Jamie Gergen, Alex Hafner, Jenelle Hagen, Muqbil Hashi, Huda Hassen, Julie Hernandez, Andrew Holm, Jennifer Hong, Michael Horn, Janey Hsu, Ryan Johnson, Ross Jorenby, Aaron Kanter, Uti Kar, Korey Karls, Sony Keo, Matthew Keefe, Grace Kim, Nicholas Kim, Sharif Koep, Jonathan Lam, Michele Larson, Jingjing Li, Jia Shin Lim, Andressa Lunardelli, Maisy Liao, Amanda Mangan, Abraham McEathron, Sarah Mattson, Brandon Meyer, Nick Midthune, Eric Miscke, Steven Mooney, David Moreno, Frances Morris, Daniel Murphy, Alexander Nelson, Cassie Nigh, Ayoung Noh, Sara Olson, Beryl Ombaso, Triana Pham, Bobby Poe, Andrew Presley, Kyle Pribyl, Jeremy A. Ragan, Xochitl Ramirez, Alana Reince, Brent Seager, Jean Seidel, Amit Shawney, Erik Skillrud, Nathan Smith, Vladimir Spasojevic, Cory Stephanson, Mark Swift, Nicki Teig, Erik Warg, John W. Thompson, Michele Trach, Suzzane Trach, Lam Tu, Adria Tyndall, Mike Vosberg, Michael Wadsworth, Takia Washington, Isaac Willard, Ryan Wilson, Nathan Wittenberg. Undergraduate Research Assistants who attended other institutions (10): Annmarie Argiros (LANDO-REU), Chanda Ciriacks (LANDO-REU), Leon Clark Jr. (MCCHD), Olivia Cyrankosky (Macalester College), Adriann Hovey (LANDO-REU), Jessica Nielson (LANDO-REU), Sana Pasha (MSERC), Britt Peterson (LANDO-REU), Angie Strack (LANDO-REU), Melanie Wijetunga (LANDO-REU) Research Assistants (3): Dmitry Andreyev, Ryan Johnson. Katherine Berning

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Teaching Service – Guest Lectures

Semester Course No. Course Title Evaluation* Fall 1998 - to

Date Chem 4094 Directed Research

Fall 1998 (Quarter)

CHEM 5133 Chemical Instrumentation and Analysis Lecture

4.4 average 4.5

Winter 1999 (Quarter)

CHEM 8992 Special Topics in Chemistry: Bioanalytical Chemistry Course Development and Teaching

4.9 average 5.5

Fall 1999 CHEM 4101 Intermediate Analytical Chemistry

5.2fl average 4.5

Spring 2000 Teaching Release, new Assistant Professor Fall 2000 CHEM 4101 Intermediate Analytical Chemistry 5.8

average 5.3 Fall 2000 IT 1910-9 Freshman Seminar, "Miniaturization: Our World Under

The Microscope" Course Development and Teaching

6.0 av. not available

Spring 2001

CHEM 8157 Bioanalytical Chemistry Course Re-structuring and Teaching

6.0 average 5.6

Fall 2001 CHEM 4101 Intermediate Analytical Chemistry

6.0fl average 4.8

Fall 2001 IT 1910-9 Freshman Seminar, "Miniaturization: Our World Under The Microscope"

6.0 av. not available

Spring 2002 Teaching Release, earlier double teaching load Fall 2002

CHEM 2111 Analytical Chemistry Laboratory

Adaptation of new experiments 5.6, 5.7

average 5.4 € Spring 2003 CHEM 8157 Bioanalytical Chemistry 6.3

average 6.5 Fall 2003 On NIH Career Award

Spring 2004 CHEM 8157 Bioanalytical Chemistry 5.4 average 5.0

Fall 2004 CHEM 8157 Bioanalytical Chemistry 5.7 average 6.0

Spring 2005 On NIH Career Award Fall 2005 On Sabbatical

Spring 2006 On Sabbatical Fall 2006 On NIH Career Award

Spring 2007 CHEM 8157 Bioanalytical Chemistry 5.2 average 6.07

Fall 2007 On NIH Career Award Spring 2008 CHEM 8157 Bioanalytical Chemistry [5.0, 5.0] ‡ Spring 2008 MedChem5494 Bioanalytical Chemistry (4 guest lectures, Dept. of

Medicinal Chemistry)

Fall 2008 CHEM 4101 Intermediate Analytical Chemistry Lecture [5.0, 5.0] ‡ Spring 2009 CHEM 8153 Extracting Signal from Noise [6.0, 5.0] ‡

Fall 2009 CHEM 4101 Intermediate Analytical Chemistry Lecture [5.5, 5.0] ‡ Spring 2010 CHEM 8157 Bioanalytical Chemistry [6.0, 5.0] ‡

Fall 2010 CHEM 4101 Intermediate Analytical Chemistry Lecture [5.5, 5.0] ‡ Spring 2011 CHEM 8157 Bioanalytical Chemistry [6.0, 5.0] ‡

Fall 2011 CHEM 4101 Modern Instrumental Methods of Chemical Analysis Lecture

[6.0, 5.0] ‡

Spring 2012 CHEM 4111 Modern Instrumental Methods of Chemical Analysis [5.0, 5.0] ‡

Edgar A. Arriaga

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Laboratory-Section 1 Spring 2012 CHEM 4111 Modern Instrumental Methods of Chemical Analysis

Laboratory-Section 3 [6.0, 6.0] ‡

Fall 2012 CHEM 4101 Modern Instrumental Methods of Chemical Analysis Lecture

[6.0, 6.0] ‡

Spring 2014 CHEM 4111 Modern Instrumental Methods of Chemical Analysis Laboratory-Section 1

[5.2, 5.0] ‡

Spring 2014 CHEM 4111 Modern Instrumental Methods of Chemical Analysis Laboratory-Section 3

[5.3, 4.7] ‡

Fall 2014 CHEM 4101 Modern Instrumental Methods of Chemical Analysis Lecture

[5.1, 5.5] ‡

Spring 2015 CHEM 4111 Modern Instrumental Methods of Chemical Analysis Laboratory-Section 1

[5.3, 4.9] ‡

Spring 2015 CHEM 4111 Modern Instrumental Methods of Chemical Analysis Laboratory-Section 3

[5.3, 4.6] ‡

Fall 2015 CHEM 1061 Chemical Principles I [4.8, 3.7] ‡ Spring 2015 CHEM 4111 Modern Instrumental Methods of Chemical Analysis

Laboratory-Section 3 [5.4, 4.4] ‡

Fall 2015 CHEM 1061 Chemical Principles I [4.7, 3.6] ‡ *Teaching Ability in Student Formal Evaluation; 1 low, 7 high. Average refers to the weighted average of other courses taught at the same level in the Department of Chemistry. fl Highest score among instructors teaching at the same level. €Two sections were taught. Evaluations may also refer to TA ability. ‡ New Student Rating system; numbers in brackets represent the median of the answers to the questions: (a) The instructor was well prepared in class and (b) The instructor presented the subject matter clearly. Answer key: 1=strongly disagree, 6=strongly agree. • Prepared "Writing Intensive" Proposal for CHEM 4111. (Spring 1999). • Prepared and evaluated questions to be included in the GRE exam for Biochemistry. (Spring 2007). • Evaluated suitability of Biochemistry ACS exam as a Proficiency Exam for incoming Chemistry Graduate

Students.

Edgar A. Arriaga

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Discipline-Related Services

Referee of research articles submitted to the following journals: • ACS-Nano • The Analyst • Analytica Chimica Acta • Analytical Chemistry • Analytical and Bioanalytical Chemistry • Bioanalytical Chemistry, Biochemistry • Biomembranes • Biotechnology • Clinical Chemistry • Current Analytical Chemistry • Diabetes • Electrophoresis • Experimental Gerontology • Expert Opinion in Medical Research • FEBS Letters • Histology and Histopathology • IEEE Sensors • Investigative Ophthalmology & Visual Science • Journal of Biotechnology • Journal of Chromatography A • Journal of Chromatography B • Journal of Gerontology A • Journal of Microbiological Methods • Journal of Pharmaceutical and Biochemical Analysis • Journal of Pharmaceutical Sciences • Journal of Physical Chemistry • Journal of Proteome Research • Journal of Separation Science • Journal of the American Chemical Society • Lab on a Chip • Langmuir • Mitochondrion • Molecular Biosystems • Molecular Pharmaceutics • Nature • Nature Nanotechnology • Nature Protocols • Proceedings of the National Academy of Sciences • Rapid Communications in Mass Spectrometry • Spectroscopy • Talanta

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Referee of research proposals submitted to the following organizations: • Defense R&D Canada, June 2004, June 2008 • Institute of Translational Health Sciences (funded by NIH), June 2008. • Iowa Center for Advanced Neurotoxicology (ICAN), January 2009. • National Research Council of Canada, January 2005, January 2007 • National Science Foundation (October 2000, April 2001, February 2002, March 2002, October

2002, April 2003, April 2004, May 2004, March 2007, September 2007) • Australian Science Fund (May 2006) • Petroleum Research Corporation (September 2002, September 2003) • Research Corporation (July 2000, May 2001, November 2001, December 2002, July 2003). Referee and member of review panels: • NIH ZRG1 CB-D "Exceptionally Innovative tools and Technologies for Single Cell Analysis" Study

Section, April 2012 • NIH NIA-B Study Section, ad hoc, February 2012, June 2012, October 2012 • NIG NIHGRI Centers, November 2011 • NIH EBT Study Section, ad hoc Chair, June 2009, June 2010, Jan 2011 • NCI Special Emphasis Panel, Session ZCA1 RTRB-2 (M1), March 2010. • NIH Recovery Act Grants (Challenge Grants), June 2009 • NIH Radiological Research Accelerator Facility Review Panel, March 2009. • NIH EBT Study Section, Permanent Member, October 2007 to 2011. • NSF-Centers for Research in Chemistry, May 21-22, 2007. • NIH Asynchronous Electronic Discussion of Instrumentation Grant Requests (S10): Mass

Spectrometers used in Proteomics approaches, July, 2007. • NIH-Enabling Bioanalytical and Biophysical Technologies Study Section, EBT Review Group,

June 11-12, 2007. • NIH-Alcohol Abuse and Alcoholism Special Emphasis Panel, Session ZAA1 DD(50), November

2005. • NIH-DDR Nanoparticles Review Panel, October, 2005. • NIH-DDR Review Panel, March 3-4, 2005. • Fondation canadienne pour l’innovation, September 18, 2003. • NIH-PPG Review Panel, September 3, 2003. • NIH-SBIR Review Panel, July 9-10, 2003. • NIH-PPG Review Panel, December 1, 2002. Referee of tenure and promotion dossiers • Southern Illinois University, October 2008. • Indiana University - Purdue University Indianapolis, July 2009. • University of Miami, September 2010. • Texas Tech University, September 2010. • University of California, Riverside, September 2011. • University of Toledo, OH, August 2013. • Thompson Rivers University, Kamloops, BC, Canada, November 2015. Organized Symposia and Conference Committees • Session, “Electrokinetic Behavior of Microparticles and Nanoparticles: Fundamentals and Applications” (Co-organizer and Co-chair with Mark Hayes), American Electrophoresis Society Meeting, October 2011. • Session, "Large-Scale Research Approaches for Enhancing Healthspan" (Co-organizer), The Next Step in Aging Research: From Bench to Bedside, June 2010.

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• Poster Awards Committee (Organizer and Chair), Microscale Bioseparations & Methods for Systems Biology Conference, MSB 2009 • Sympsium “Cellular and Sub-Cellular Separations” (Co-organizer and Co-chair with Dimitri Pappas), Pittcon 2009 • Symposium “2008 Minnesota Aging Retreat”, Center on Aging, 2008 • Poster session (Poster judge), Design of Medical Devices Conference 2007 • Session IV (Chair), LACE 2007 • Symposium "Subcellular Proteomics" (Organizer and Chair), Pittcon 2005 • Symposium "Separation Techniques for Biological Nanostructures" (Organizer and Chair), Pittcon 2004 • Symposium "Emerging Strategies for Subcellular Analysis" (Organizer and Chair), Pittcon 2003 • Session “Capillary Electrophoresis” (Co-organizer and Co-chair with Mike Bowser), Minnesota Chromatography Forum Spring Symposium, MCF 2001 • Symposium “International View on Chemistry Education” (Co-Organizer and Presider), 248th ACS National Meeting.

Departmental and University Service/Committees • Procter & Gamble Workshop, “Careers as Analytical Chemists in Industry,” Co-organizer, October

19, 1999. • Department of Chemistry Research Symposium, New Graduate Student Orientation, Co-

organizer, September 1, 1999 • Department of Chemistry Recruiting Weekend, Poster Session Coordinator, Spring 2000. • Biotechnology, and Chemistry-Biology Interface, Molecular Biophysics, and the Center for

Microbial Genomics Joint Training Grant Symposium, “Drug Design and Genomics,” Poster Session Coordinator, May 5-6, 2000.

• Analytical Chemistry Seminar, Coordinator (with Eric Munson), Department of Chemistry, September 2000 – June 2001.

• Research Symposium, New Graduate Student Orientation, Coordinator (with Darrin York), Department of Chemistry, August 31, 2000.

• Seminar Committee, Member, Department of Chemistry, September 2000 – June 2001. • Graduate Curriculum Committee, Member, Department of Chemistry, September 2000 – June

2001. • Graduate Admissions Committee, Member, Department of Chemistry, September 2001 – May

2005. • Analytical Specialty Area Coordinator, Department of Chemistry, Spring 2001 to Spring 2003. • Molecular Biophysics, Biotechnology, and Chemistry-Biology Interface Joint Training Grant

Symposium, “Chemical Biology, A Single Molecule Perspective,” Organizer, May 30, 2002. • UROP Proposal Review Committee, Institute of Technology, Member, September 2002 – May

2003. • Faculty Search Committee: Science Education, Department of Curriculum and Instruction,

Member, October 2002 to October 2005. • LANDO Applications Review Committee, Member, Department of Chemistry, January – May

2005. • Mentoring Tenure Committee (C. Haynes), Member, Department of Chemistry, September 2005 -

present. • Chemistry-Biology Interface Training Grant, Steering Committee, Member, December 2006 -

present. • Cargill Endowed Chair in Metabolomics Search Committee: Department of Biochemistry,

Molecular Biology and Biophysics, Member, September 2006 to present.

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• Graduate Symposium, Judge, Department of Chemistry, May 2007. • Writing Workshop Mentor, Ad Hoc, Department of Chemistry, September 2007. • Planning, Staffing, and Resources Committee, Member, Department of Chemistry, September

2007 – August 2010. • Faculty Search Committee, Member, Department of Chemistry, September 2007 – February

2008. • 2008 Minnesota Aging Retreat, Organizer, Center on Aging, University of Minnesota, January

2008. • Scientists in Aging Research Monthly Meetings, Organizer, Center on Aging, University of

Minnesota, January 2008 – September 2008. • Underrepresented Groups Recruitment Committee, Co-organizer, Chemistry-Biology Interface

Training Grant, February 2008 to present. • Underrepresented Groups Recruitment Committee, Co-organizer, Biotechnology Training Grant,

April 2008 to present. • Mass Spectrometry Facilities Director, Search Committee, Member, Department of Chemistry,

June 2008 - May 2009 • Analytical Specialty Area Coordinator, Department of Chemistry, Spring 2009 - Spring 2012. • Biotechnology Training Grant, Steering Committee, Member, May 2009 - present. • Center on Aging, Ad hoc Fesler-Lampert Chair Selection Committee, May 2009. • Analytical Specialty Area Coordinator, Department of Chemistry, Spring 2009 - present. • Graduate Student Workshop, Department of Chemistry, Faculty Panel Participant, May 2010. • Tenure and Promotion Committee, Member, Fall 2010 – present. • Graduate Symposium, Judge, Department of Chemistry, May 2010. • Center for Mass Spectrometry and Proteomics, Internal Review Board, Fall 2011. • President’s Distinguished Faculty Mentor Program, Fall 2011 – • Masters of Biological Sciences, Graduate Faculty, Fall 2011 – • Graduate Curriculum Committee Member, Fall 2014 – • Diversity Committee, Fall 2015 – • Medical Discovery Team: Biology of Aging, Search Committee, Member, Fall 2015 -

Outreach Related Activities

• Advisor to the Professional Chemical Fraternity, AKS, β Chapter (October 1998 – September 2005)

• Judge for High School Science Project Competitions, Minnesota Academy of Science (Spring 1999)

• Organizer of workshop: "Solving Analytical Problems in Industry", offered by Procter and Gamble to Midwest Undergraduates (October 1999)

• Active Participant in National Chemistry Week, 2000, 2001 • Active Participant in "El Dia del Niño", Minnesota Science Museum, Saint Paul, MN (May, 2000) • Host for Visiting Scientists: (1) Professor David Samuels, University of Newcastle, United

Kingdom, August – December 2001; (2) Professor Scott Hartsel, University of Wisconsin–Eau Claire, September 2002 – August 2003 (sponsored by RSEC); (3) Professor David Lewis, University of Wisconsin–Eau Claire, September 2004 – August 2005 (sponsored by RSEC); Professor Gary Mabbott, University of Saint Thomas, September 2005 – August 2006 (sponsored by RSEC).

• Organizer of Minnesota Analytical Professors Annual Meeting (MAP) (May 2001)

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• Active Participant in Converciencia 2005, scientific exchange between representatives of the government of Guatemala, Guatemalan industry, and officials and students from the following Guatemalan universities: Universidad San Carlos de Guatemala, Universidad Mariano Galvez, Universidad Rafael Landivar, Universidad Francisco Marroquin, and Universidad del Valle de Guatemala, Guatemala City, Guatemala, July 4 - July 7, 2005.

• Cofounder and Member, "La red de scientificos que viven en el exterior" (Network of Guatemalan Scientists Living Abroad), July 7, 2005 - present.

• Judge of Posters presented at the Design of Medical Devices Conference, Minneapolis, MN (April 2007).

• Mentor, Ad Hoc, “The Davidson Institute for Talent Development”, Annual Meeting, Saint Paul, MN, June 22, 2007.

• Mentor, Ad Hoc, “Amantes de la Ciencia”, Minnesota Science Museum, Saint Paul, MN (April 2008).

• Representative of the Department of Chemistry Biotechnology Training Grant and the Chemistry-Biology Interface Training Grant at diversity-focused undergraduate conferences: (1) CIC Summer Research Opportunity Programs, MSU, Lansing, Michigan, July 2008; (2) Annual Biomedical Research Conference for Minority Students, Orlando, Florida, October 2008, (3) CIC Summer Research Opportunity Programs, University of Michigan, Ann Arbor, Michigan, July 2009; (4) Annual Biomedical Research Conference for Minority Students, Phoenix, Arizona, October 2009; (5) CIC Summer Research Opportunity Programs, Ohio State University, Columbus, Ohio, July 2010; (6) Annual Biomedical Research Conference for Minority Students, Charlotte, North Carolina, November 2010.

• Coordinator of the diversity initiatives of the Biotechnology and the Chemistry-Biology Interface Training Grants, University of Minnesota, January 2008.

• Mentor, Ad Hoc, “Amantes de la Ciencia”, Minnesota Science Museum, Saint Paul, MN (April 2009).

• Chemistry coordinator, IT tour by Edison High School, Hispanic students (April 2009). • Participant, faculty panel, North Star STEM Alliance student research symposium, University of

Minnesota, March 2010. • Mentor, Ad Hoc, “Amantes de la Ciencia”, Minnesota Science Museum, Saint Paul, MN (April

2010). • Mentor, Society of Hispanic Professional Engineers, Student Chapter, University of Minnesota

(May 2010 - ) • Mentor, Ad Hoc, “Amantes de la Ciencia”, Minnesota Science Museum, Saint Paul, MN (January

2011). • Mentor, “President’s Distinguished Faculty Mentor Program”, University of Minnesota, MN (2011-

2012) • Mentor, Ad Hoc, “Amantes de la Ciencia”, Minnesota Science Museum, Saint Paul, MN (January

2012). • Panelist, Ad Hoc,”Recommendation Letters”, North Star STEM Alliance, Minneapolis, MN

(February 2012). • Panelist, Ad Hoc, “Tips and strategies for navigating graduate school”, Community of Scholars

Program, University of Minnesota, MN (March 2012). • Panelist, Ad Hoc, "2nd STEM Exploration Day Lunch and Informational Session", North Star

STEM Alliance, University of Minnesota, MN (November 2012).