Dream Team Lab Project

7/29/2019 Dream Team Lab Project

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Dream team

Easier to digest;Introduction and plan


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Group rules and roles

Rules RoleAida

LizJesJay


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Experiment 1 quick description andguaiacol safety

observing the effects of differentconditions such as pH,temperature concentration etc;on the rate of catabolicbreakdown of peroxide by theenzyme peroxidase from turnips.

RH2 + H2O2 -----------> R+ 2 H2O

reducing agent = guaiacol

guaiacol

guaiacol

Analysis: guaiacol is a colourless solustate, but changes colour when it is ocan measured using a spectrophoto

Safety:eyes; guaiacol is an irritant and will cand pain. [wear goggles]skin; causes irritation to skin. [ensure scompletely down, wear gloves and shoes]ingestion; Guaiacol is harmful if ingesdrink, make sure to not touch your mhandling guaiacol and wash hands iinhalation; can be dangerous if inhause a fume cupboard]

references: http://www.ch.ntu.edu.tw/~genchem99/msdshttp://www.sciencelab.com/msds.php?msdsId
http://www.ch.ntu.edu.tw/~genchem99/msds/exp23/guaiacol.pdfhttp://www.sciencelab.com/msds.php?msdsId=9924208http://www.sciencelab.com/msds.php?msdsId=9924208http://www.ch.ntu.edu.tw/~genchem99/msds/exp23/guaiacol.pdf


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H2O2 safety

Much like guaiacolHydrogen peroxide isan irritant, soprotective eyegear,handgear andclothing must be

warn, and care mustbe taken not to ingestor spill duringhandling.


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Experiment 1 method (finding the enzyme baseline)

Equipment required:

Pipettes (10ml & 5ml&1ml )Pipette tips (10ml &

5ml& 1ml )7 test tubes

1 small beakerA test tube rack

bottle of distilled water

cuvettes

reagents required:H202 0.1%

concentration (3mls)guaiacol (3mls)

turnip extract (5mls)

fill 3 test tubes with the reagents shown above and label th

stock reagents, place them in a test tube rack. Fill a small bwater, and then label a fourth test tube reactio

label 3 test tubes 1 2 and 3 and and fill them with the reagtable in the handout.

transfer some solution from tube 1 into a cuvette anspectrophotometer. ( we use a reagent blank as, although

look clear it may be slightly tinted and will therefore have a to clear water, i.e. it prevents zero error of the spectro

mix tubes 2 and 3 to make the sample mixture and quickly acuvette this will be t = 0 then record the absorption at 500 nm

until 2 minutes is reached.

then plot a graph of absorption against time. Absorption on

on the X


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Results table and graph


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Does the amount of enzymeinfluence reaction rate?

Prediction:

Procedure: Follow theprocedure forexperiment 1, but this

time change the turnipextract concentration

and measure theeffects. (double and

half it)

reference

tables inhandout


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Our independently planned experiment.Does _____________ influence reaction rate?

prediction:

procedure:


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Data:

Documents

Dream Team Lab Project