8/9/2019 Dissection of the Genetic Basis of Heterosis in an
Elite Maize Hybrid by QTL Mapping in an Immortalized F2
Populati
5/8
Binkh pmc pmvcc |lcg` oniznkckps' cbv gckhpm mb` b mlhm
mcvlpbflglp| -==+:#, bk` mlhmcsp il )zbvckp mcpcvnsls
-58+3#,+ B``lplnkbgg|' lp mb` slhklflobkp onvvcgbplnk xlpm
hvblk |lcg` -v9 :+55' ZW :+:2,+ Pmcvcenvc' cbv gckhpm xbs
pback bs bk c}bizgc pn lggspvbpc pmc ceecops ne `lhcklo
lkpcvboplnks onkpvlfplkh pn il`)zbvckp mcpcvnsls+ Lk pmc BB
lkpcvboplnk p|zc -`bpb knp smnxk,' insp onizgcickpbv|
mnint|hnpcs -22.66 nv 66.22, mb` `lsplkop b`ubkpbhcs nucv
pmc icbks ne pxn zbvckpbg hcknp|zcs -22.22 bk` 66.66,' bk`
xcvc bgxb|s pmc fcsp hcknp|zcs+ Bgpmnhm ibk| B@.@B
lkpcvboplnks ne pxn gnol mb` `lsplkop b`ubkpbhcs nucv pmc
icbks ne pmc pxn zbvckpbg hcknp|zcs -22.22 bk` 66.66,' nkg|
io2805
zml03?2626+ :+=6 oi, bk` pms
boonkpc` nkg| env ?2+0# ne il`)zbvckp mcpcvnsls env pmls
pvblp+ Pmls vblsc` pmc rcsplnk ne mnx pn c}zgblk pmc vcsp ne
pmc mcpcvnsls lk pmc E2 m|fvl`+ Lk pmls sp`|' pmc onkpvlf)
plnks pn mcpcvnsls evni bgg pmvcc p|zcs ne `lhcklo lkpcvbo)
plnks xcvc pcspc` lk pmc LE6 znzgbplnk nk pmc fbsls ne
zng|invzmlo gnol lk E2+ Binkh pmc pmvcc p|zcs ne `lhcklo
lkpcvboplnks' BB ceecops xcvc ne hvcbpcsp liznvpbkoc+ Bs
`cinkspvbpc` f| Icgomlkhcv cp bg+ -6::8f,' pmc| onkpvlfpc
Pbfgc 6 Mcpcvnplo gnol -MG, l`ckplflc` env hvblk |lcg` bk` lps
oniznkckps lk pmc liinvpbgltc` E6 znzgbplnk `cvluc` evni pmc cglpc
ibltc m|fvl`
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MG Egbkalkh ibvacvs @lspbkoc -oI,b
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8/9/2019 Dissection of the Genetic Basis of Heterosis in an
Elite Maize Hybrid by QTL Mapping in an Immortalized F2
Populati
7/8
pn il )zbvckp mcpcvnsls ulb pmc bhickpc` `nilkbkoc
ceecops+ Evpmcvinvc' xc obkknp vgc np pmbp mlhmcv)nv`cv
lkpcvboplnks binkh invc pmbk pxn gnol ib| mbuc bgsn
bzzvcolbfgc ceecops nk mcpcvnsls+
B onizbvlsnk ne pmc vcsgps lk pmls sp`| xlpm pmnsc lk
vloc vcznvpc` f| Mb cp bg+ -6::?,' fnpm onk`opc` f| slkh
LE6 znzgbplnks' vcucbgc` snic spvlalkh oniink ecbpvcs
env pmc hckcplo fbsls ne mcpcvnsls fcpxcck ibltc bk` vloc+Bp pmc
pxn)gnos gcucg' b gbvhc kifcv ne `lhcklo lkpcvbo)
plnks' xmlom onkpvlfpc` pn mcpcvnsls env hvblk |lcg` bk` lps
oniznkckps' xcvc `cpcopc` lk fnpm ovnzs+ Bgg pmvcc alk`s ne
`lhcklo lkpcvboplnks' BB' B@.@B bk` @@' xcvc `cpcopc`
xlpm BB lkpcvboplnks mbulkh pmc mlhmcsp bk` @@ lkpcvbo)
plnks pmc gnxcsp evcrcko| lk vloc bs xcgg bs lk ibltc+ Invc
lkpcvcsplkhg|' fbsc` nk pmc onizbvbpluc hckcplo ibz ne
ocvcbg ovnzs' klkc MG env hvblk |lcg` bk` lps oniznkckps
`cpcopc` f| s lk ibltc bvc gnobpc` lk slilgbv vchlnks ne
onvvcsznk`lkh omvninsnics ibzzc` f| Mb cp bg+ -6::?,
lk vloc -`bpb knp smnxk,+ Env lkspbkoc env hvblk |lcg`' pmc
omvninsnic vchlnk ne MG h|2 `cpcopc` nk omvninsnic 2ne ibltc
onvvcsznk`s pn pmbp ne MG |`? `cpcopc` nk pmc
omvninsnic ? ne vloc+ Pmls lizglcs pmbp ibltc bk` vloc
ib| smbvc snic ongglkcbvlp| ne mcpcvnplo gnol bp pmc slkhgc)
gnos gcucg+ Mnxcucv' bgg MG bp pmc slkhgc)gnos bk` pmc @@
lkpcvboplnks bp pmc pxn)gnol gcucgs boonkpc` env =5# mcp)
cvnsls env hvblks zcv zbklogc lk vloc m|fvl` _mbk|n 5?
-Mb cp bg+ 6::?,+ Lk onkpvbsp' pmcsc pxn p|zcs ne hckc
boplnk ong` nkg| c}zgblk ?0+2# mcpcvnsls env cbv gckhpm lk
ibltc m|fvl` \|66+
Bknpmcv lkpcvcsplkh ecbpvc vcucbgc` f| pmls sp`| xbs
pmbp env |lcg` oniznkckps' pmc gbvhcsp kifcv ne MG xbs
`cpcopc` env cbv gckhpm pmbp mb` pmc mlhmcsp il )zbvckp
mcpcvnsls' bk` b sibggcv kifcv ne MG xbs enk` env cbv
vnx kifcv bk` 2::)acvkcg xclhmp' xmlom mb` gnxcv mcp)
cvnsls+ Pnhcpmcv xlpm pmc RPG ibzzlkh vcsgps evni VLGs'
pmcsc vcsgps shhcsp pmbp pmc hckcplo fbsls k`cvg|lkh pmc
pmvcc ibdnv |lcg oniznkckps bvc `lsplkop+ Xmlgc pmc
lkmcvlpbkoc ne cbv gckhpm lk m|fvl` xbs onkpvnggc` f|
b``lpluc bk` knk)b``lpluc ceecops' b``lpluc ceecops iblkg|
onkpvlfpc` pn pmc lkmcvlpbkoc ne cbv vnx kifcv bk` 2::)
acvkcg xclhmp+ Pmnhm hvblk |lcg` mb` pmc mlhmcsp mcpcvnsls
binkh pmc env icbsvc` pvblps' nkg| pmvcc MG xcvc
l`ckplflc`' zvnfbfg| fcobsc pmcsc mb` pmc mlhmcsp hckcplo
onizgc}lp|+
Nv vcsgps ib| mbuc fcck beecopc` f| pmc sc ne b vbk)
`niltc` fgnoa `cslhk' fcobsc szbplbg ubvlbflglp| xlpmlk
gbvhc fgnoas ib| mbuc onkpvlfpc` pn pmc c}zcvlickpbg cvvnv
ubvlbkoc+ Bknpmcv onizglobplnk xbs pmbp pmc c}zcvlickps
xcvc knp fbgbkoc`' fcobsc b sibgg kifcv ne ovnsscs xcvc
ilsslkh -%?+3#,+ Onksl`cvlkh pmc mlhm mcvlpbflglplcs lk nv
sp`|' xc onkog`c` pmbp pmcsc `vbxfboas mb` nkg| b ilknv
ceecop nk nv vcsgps+ Invc liznvpbkpg|' nv icpmn` ne
csplibplkh pmc vbpln `.b `lvcopg| evni pmc csplibpcs ne pmc
kicvbpnv bk` `cknilkbpnv ib| mbuc vcsgpc` lk flbsc`
csplibpcs+ Skflbsc` csplibpcs env pmc vbpln `.b ong` fc
nfpblkc` f| slkh b knk)glkcbv in`cg' lk xmlom pmc `nil)
kbkoc ceecop ls c}zvcssc` bs b zvn`op ne pmc `nilkbkoc
vbpln bk` pmc b``lpluc ceecop b vbpmcv pmbk pmc glkcbv in`cg
sc` f| s+ Mnxcucv' pmls xng` vcrlvc pmc `cucgnzickp ne
kcx snepxbvc env RPG bkbg|sls+ Invcnucv' lk obscs xmcvc
pmc b``lpluc ceecop b ls knp slhklflobkpg| hvcbpcv pmbk
tcvn'pmls p|zc ne knk)glkcbv in`cg obkknp fc bzzglc`+ Lk szlpc
ne
pmcsc glilpbplnks' nv sp`| zvnul c` sceg lkenvibplnk nk
pmc kbpvc ne hckc boplnk onkpvlfplkh pn mcpcvnsls bs xcgg bs
MG env hvblk |lcg` bk` lps oniznkckps+ Pmcvc xcvc b gbvhc
kifcv ne `lhcklo lkpcvboplnks onkpvlfplkh pn mcpcvnsls+
Pmcsc lkunguc` bgg pmvcc alk`s -BB' B@.@B' @@, ne lkpcv)
boplnks' fp BB lkpcvboplnks xcvc invc evcrckp+ Evpmcv'
nv sp`| shhcspc` ongglkcbvlp| ne MG bp pmc slkhgc)gnos
gcucg fcpxcck ibltc bk` vloc+
Boaknxgc`hickps Pmls xnva xbs szznvpc` f| b hvbkp evni pmc
_pbpc Ac| Fbslo Vcscbvom bk` @cucgnzickp Zgbk ne Omlkb+
F+_+@mlggnk bk` B+C+ Icgomlkhcv xcvc flkbkoc` f| pmc @cpsomc
Envs)
omkhshciclksombep -@EH, xlpmlk pmc zvlnvlp| zvnhvbi _ZZ 220
