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PCM124202EN00 (12/20)
Direct Swab Preparation – Deepwell Format
Amplification and Detection Procedure
Open Master Mix slowly. Add 135 µL of Rehydration Solution. Replace cap
and allow it to sit for 1-2 minutes. Gently pipette rehydrated Master
Mix up and down 3-5 times.Avoid creating bubbles.
12x Master Mix Rehydration SolutionNegative ControlPositive Control
Kit Contents
96
Emergency Use Only
Process Buffer
1
Pipette 400 µL of the Process Buffer into each deepwell as needed.
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Heat the deepwell plate for 10 minutes at 95°C at 300 rpm.
Amplification and DetectionSample Preparation
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45mL
External Controls
To process the samples, add patient swab(s) into each deepwell. Vigorously twirl swab for 10 seconds in Process Buffer. Roll the swab head against the inside of the tube wall as you remove it.
Dispose of used swab in biohazard waste container.
3H
GF
ED
CB
A
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Remove the deepwell plate from the heating block and place onto the frozen cooling block at room
temperature for 10 minutes.
Direct Swab withDeepwell Format
Procedure Card
Insert PCR plate into appropriately programmed thermocycler and
select appropriate Lyra assayprotocol. Approximately
70 minutes per run.
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45mL
HG
FE
DC
BA
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To process the controls add 50 µL of Positive and Negative Control
to the designated deepwell.
HG
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DC
BA
HG
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Mix each deepwell (column or row) 3 times using a pipette set at 150uL. Immediately transfer 5 μL of sample to the PCR plate.
Repeat as needed. Seal the PCR plate. Centrifuge PCR plate for 15 seconds.
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HG
FE
DC
BA
Study the Package Insert and Technical Bulletin thoroughly before using this Procedure Card. This is not a complete Package Insert.
AB
CD
EF
GH
Place the microwell PCR plate on the frozen microwell plate cooler.
Add 15 μL of Master Mix to each well.
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