Detoxification: A Comprehensive Approach Made Simple* · Detoxification: A Comprehensive Approach Made Simple* Kelly C. Heim Ph.D. *These statements have not been evaluated by the

Detoxification: A Comprehensive

Approach Made Simple*

Kelly C. Heim Ph.D.

*These statements have not been evaluated by the Food & Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Kelly C. Heim Ph.D.

I am a paid advisor to Pure Encapsulations. I have no other conflicts of interest to disclose.

The Company assumes no liability for presenter’s information, whether conveyed verbally or in these materials. All presentations represent the opinions of the presenter and do not represent the position or the opinion of the Company. Reference by the presenter to any specific product, process or service by trade name, trademark, or manufacturer does not constitute or imply endorsement or recommendation by the Company.

Seminars, teleconferences and webinars such as this are for educational purposes only and are intended for licensed health care practitioners.

These therapies are not substitutions for standard medical care. Practitioners are solely responsible for the care and treatment provided to their own patients.

Speaker disclosure

"Poison is in everything, and

no thing is without poison.

The dosage makes it either a

poison or a remedy."

Sustaining Life

• Combinations and/or libraries of

phytochemicals are the most

effective plant defenses

• Diversity can negotiate uncertainty

• Diversity can counter threats that

are unprecedented

Diversity = Defense

Xenobiotics are chemicals that are

not made by the body and are

therefore foreign.

• Plant metabolites (e.g. psoralens

and pthalides)

• Heavy metals

• Environmental contaminants

• Industrial toxins

• Drugs

Exogenous Toxins (Xenobiotics)

Benzene

Insidious biochemicals generated by the

body:

• Hormones

• Reactive metabolites

Endogenous toxins

Estradiol

Metabolism of toxins occurs

primarily in the intestinal wall and

liver

• Phase I creates a site for the

subsequent addition of a polar

molecule

• Phase II adds the polar

molecule, rendering the toxin

less active and more soluble,

enabling excretion

• Phase III is the excretion

Biotransformation

• B-complex vitamins

• Phytochemical inducers

• Antioxidants

Cytochrome p450 • Usually more soluble

• Often very reactive

Phase I: Site Creation

TOXIN TOXIN

• Nutritional cofactors

Phase II Enzymes

• Amino acids

• Glutathione

TOXIN TOXIN

Phase II: Conjugation

C

• Phase II enzyme

• Conjugates estrogens and

environmental toxins

Glutathione Sulfotransferases (GST)

TOXIN

Less reactive

Easily excreted

TOXIN

GST

GSH

Reaction Enzyme Nutritional support*

Glutathione conjugation GSTs Glutathione, glutamine,

NAC, ALA, glycine, folate*

Amino acid conjugation Aminotransferases Cysteine, glycine*

Methylation COMT Methionine, folate,

methylcobalamin *

Sulfation

Glucuronidation

SULTs

UGTs

Isothiocyanates,

flavonoids, curcuminoids,

triterpenoids, diallyl

disulfide*

Phase II is highly responsive to nutritional support

*These statements have not been evaluated by the Food & Drug Administration. These products are not intended to diagnose, treat, cure or prevent any disease.

Efflux Pump

Elimination

TOXIN TOXIN

Cell

Phase III: Efflux

INTESTINAL CELL MEMBRANE

Phase III

INTESTINAL LUMEN

CELL INTERIOR

Phase II

TOXIN

TOXIN

C

TOXIN Phase III

Phase I

Phase III

Elimination

Overview

Which is easier?

• Cruciferous vegetables (glucosinolates)*

• Tea (catechins)*

• Berries (flavonoids)*

• Vegetables and spices*

o Polyphenols – curcuminoids, flavonoids*

o Sulfur-containing compounds – diallyl disulfide*

o Glucuronidase modulators – piperine, curcuminoids*

Phytonutrients have generalized, positive effects*


Which bioactive is best?

Sulforaphane (SFN)

Indole 3-carbinol (I3C)

3,3’-Diindolylmethane (DIM)

Glucosinolates

Nrf2: A multi-organ detoxifier

Acts in the nucleus of cells of nearly

every organ to serve a general

detoxifying role

• G.I. tract

• Kidneys

• Spleen

• Brain

• Lungs

• Skin

A genomic “switch” that activates

>1000 protective genes

Protective against:

• Toxins

• Reactive metabolites

• ROS

• Environmental xenobiotics

Nrf2

Nrf2

GSH

GST

SOD2

GPx

2

UGT

NQO1

VDR

Nrf2: Master inducer of antioxidant and detoxification

genes

UltraGSH Detox*


Nutritional support for glutathione synthesis*

SLC6A9

Cystine

Cysteine

Glutamylcysteine

Glycine GSH

GSSG

GSR

NADPH

SLC7A11

TXNRD1

GCL

L-Glutamine

NAC*

Liver G.I. Detox*


Methylated

estrogens

Estrogen Detoxification

Single nucleotide polymorphisms (SNPs)

affect the structure and function of

detoxification enzymes.

• Phase I: p450 SNPs

• Phase II:

• Glutathione transferases (GSTs)

• UDP glucuronosyltransferases

(UGTs)

• Sulfotransferases

• Antioxidant defenses (SOD2, GPx)

Genetic variations that impact detoxifying potential

Target as many pathways as possible through these 4 approaches*:

1. Develop a comprehensive diet, supplement and lifestyle program*

2. Diversify and vary dietary fruits, vegetables and spices over the long-

term*

3. Select combinations and formulas over stand-alone ingredients for

detox protocols*

4. Focus on “umbrella targets” such as Nrf2, which ramify to many

different phase II reactions and antioxidant systems

Accommodate unknown genetic variations


• Preliminary evidence that

glucuronidation increases with

fasting

• Some detoxifying phytochemicals

are more bioavailable in the

morning*

• More research is needed to clarify

clinical significance

• Best approach is to periodically

rotate the time of day of supplement

administration

Circadian influences


1. A detoxifying diet

2. Gut microbial balance

3. Gut integrity and motility

4. Heavy metals

5. Optimal biotransformation

a. Xenobiotics

b. Hormones

Detoxification: Key objectives

• Hypoallergenic

• Organically grown, natural and whole foods

• Rich in fruits, vegetables, fiber

• 8–12 oz (240-360 ml) of water, ideally 8 times per day

A detoxifying diet

Structure = Epithelial integrity

Maintain the mucosal barrier

architecture to limit passage of

toxins and allergens into the

bloodstream

Digestive health: Structure and function

Intestinal epithelium

Function = Motility

Digestive health: Structure and function

Gut microbiota

• Participate in many aspects of

xenobiotic detoxification

• Express enzymes that the human

genome does not encode

o These enzymes can transform

toxins into less insidious

metabolites

Gut microbial balance

• Lead, mercury, cadmium and

arsenic are present in

agricultural, industrial, and

residential settings

• May enter the human body

through food, water, air, or

absorption through the skin

• Are toxic when they are not

metabolized by the body and

accumulate in tissues

Heavy metals

• UltraNutrient® (3 capsules)

• EPA/DHA essentials (1 softgel)

• Liver-G.I. Detox (2 capsules)

• Alpha lipoic acid, curcumin,

broccoli, silymarin, amino acids

• NAC 600 mg (1 capsule)

• Taurine 1,000 mg (1 capsule)

Detox Pure Pack

Hypo-allergenic rice protein enhanced with

free-form amino acids, vitamins and

minerals to support detoxification*

• 15 grams protein

• B complex for phase I detoxification*

• Amino acids such as taurine, methionine,

glutamine and glycine for phase II

detoxification*

• Antioxidant support*

• Vitamins and minerals for

comprehensive support*

PureClear


Blend of key probiotic bacteria to

support gastrointestinal and

immune health*

Provides 10 billion CFU

• Lactobacillus acidophilus

• Lactobacillus rhamnosus

• Bifidobacterium longum

• Bifidobacterium lactis

• Bifidobacterium bifidum

Probiotic 5


*These statements have not been evaluated by the Food & Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

12.5 billion CFU of a 4-strain clinically researched probiotic blend with n-acetyl-l-cysteine, milk thistle and broccoli sprout concentrate

• Microbial flora can bind and transform toxins within the body to promote detoxification*

• Moderates the absorption of toxins across the intestinal barrier into the body*

• Binds and enhances the excretion of heavy metals*

• Supports phase II detoxification with sulforaphane from broccoli*

• Probiotic supplementation promotes healthy glutathione reductase activity to support optimal glutathione synthesis*

• NAC and silymarin support glutathione synthesis*

PureBi•Ome™ Detox

Supports the function, microflora balance,

integrity, tone, and detoxification of the G.I.

tract*

• Psyllium husk and flax seed powder for gut

integrity and motility*

• l-glutamine for glutathione synthesis and

gut epithelial health*

• Deglycyrrhizinated licorice (DGL),

marshmallow root, aloe (inner fillet) and

slippery elm for advanced mucosal

protection*

• Triphala to support colon detoxification*

G.I. Fortify


Powder (1 heaping scoop):

• Psyllium husk 4 g

• L-glutamine 1 g

• Aloe (inner fillet) 100 mg

• Flax seed powder 2.25 g

• DGL 600 mg

• Marshmallow root 400 mg

• Slippery Elm 200 mg

• Triphala 90 mg

G.I. Fortify

Capsules (3):

• Psyllium husk 1100 mg

• L-glutamine 500 mg

• Aloe (inner fillet) 100 mg

• Flax seed powder 400 mg

• Chlorella 100 mg

Supports detoxification and defenses against

mild exposure to common heavy metals*

• Modified citrus pectin, cilantro and

chlorella bind metals and promote their

excretion*

• NAC and selenium promote urinary

excretion of mercury and enhance

glutathione levels*

• Alpha lipoic acid promotes the biliary

excretion of inorganic mercury and

supports antioxidant protection*

• l-Methionine supports phase II

detoxification*

HM Complex


Liver-G.I. Detox

Supports multiple detox pathways in the

gut and liver*

• Alpha lipoic acid, curcumin and

broccoli for Nrf2 support*

• NAC, methionine, glutamine, glycine,

taurine provide substrates for phase II

and glutathione synthesis*

• Silymarin: promotes phase I and

phase II detox and supports

glutathione*

• Artichoke supports biliary flow*


DIM Detox

Promotes healthy estrogen metabolism*

• Supports hormonal detoxification

with BioResponse DIM®*

• Supports healthy hormone phase II

glucuronidation and sulfation*

– Sulforaphane, calcium-d-

glucarate, taurine, glycine and

NAC*

• Promotes antioxidant defenses to

protect liver cell health*


Zero Compromises.

Pure Results.

Documents

Detoxification: A Comprehensive Approach Made Simple* · Detoxification: A Comprehensive Approach Made Simple* Kelly C. Heim Ph.D. *These statements have not been evaluated by the