DArT-based linkage map of the

Solanum tuberosum ssp andigena accession LOP-868

(CIP 702853)Cultivar Name : Alca Tarma

Chromosomes I to XII

Maria V. Caraza SalasElisa J. Mihovilovich CastroMariela Aponte VilladomaMerideth Bonierbale

Notes

Population size =174 dihaploid progenies derived from haploidization of LOP 868 with haploid inducer lines IVP-101 and 596001.4 of Solanum phureja Juz. et Bukasov

Total DArT markers mapped = 521 Mean DArT markers per chromosome = 43 Number of SSR markers mapped = 45 Mean SSR markers per chromosome = 4 Additionally:

A major gene denoted Rladg for resistance to PLRV identified in this accession mapped on chromosome V (Velásquez et al. 2007)

An AFLP marker P13M34.453 mapped close to the major gene for PLRV resistance

Position of QTL for late blight resistance identified by association mapping in a Solanum t. andigena-derived B1 Population on chromosomes I, II, III; and IX

Total length of the tetraploid map = 1211.6 cM

Linkage map construction

The genetic map was constructed using the statistical program TetraploidMap (Hackett and Luo 2003)

Marker order was established by the simulated annealing algorithm of TetraploidMap at starting T = 10, a cooling factor of = 0.85 and M = 100n random changes at each T°

Chromosome assignment and polarity was established by mapping SSR markers and using “sol genomics network” maps; Potato meta-QTL consensus map and Solanum phureja diploid map 2010, as reference http://solgenomics.net/

References

Hackett CA, Luo ZW (2003) TetraploidMap: construction of a linkage map in autotetraploid species. J Hered 94(4):358–359

Velásquez AC, Mihovilovich E, Bonierbale M. (2007) Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanum tuberosum ssp. andigena. Theor Appl Genet 114 (6):1051-1058

Chromosome I

QTL for late blight resistance found in B1 population by association mapping

Chromosome II

QTL for late blight resistance found in B1 population by association mapping

Chromosome III

QTL for late blight resistance found in B1 population by association mapping

Chromosome IV

Chromosome V

Chromosome VI

Chromosome VII

Chromosome VIII

Chromosome IX

QTL for late blight resistance found in B1 population by association mapping

Chromosome X

Chromosome XI

Chromosome XII