Dark Reaction ppt

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    By: ANAZ. ANATURAL SCIENCE

    Candidate

    code:13375006

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    6CO2 +6H2O C6H12O6+6O2

    The dark reactions use the energy stored in ATP and

    NADPH the light reactions. Carbon atoms from CO2are bonded, or fixed, into

    organic compounds = carbon fixation.

    THIS OCCURS IN THE STROMA

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    STEP 1An enzyme (rubisco),

    combines CO2with a 5-carbon sugar RuBP

    The product, 6-C sugar,immediately splits into

    2, -3C molecules (PGA)

    PGAPhosphoglycericAcid

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    Step 2 PGA is converted to

    another 3- Carbonmolecule PGAL in a 2 part

    process: Each PGA receives a P

    group from ATP

    The resulting compoundreceives a proton from

    NADPH and releases theP, producing PGAL

    (ADP & NADP+ return to lightrxn., to make ATP andNADPH)

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    Step 3

    Most of the PGAL is

    converted back to RuBP Requires a P from

    another ATP

    Some PGAL leave andused by plants createorganic compounds

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    Balance Sheet for Photosynthesis How much ATP & NADH are required to make 1

    molecule of PGA from carbon dioxide?

    Each turn fixes one CO2

    PGAL is a 3-C molecule (takes 3 turns to make eachmolecule)

    Each turn of the cycle:

    3 ATP ( 2 in step 2 & 1 in step 3) 2 NADPH (step 3)

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    About 50% made to fuel cell Respiration

    Some of the PGAL is used to make amino acids, lipids,carbohydrates like glucose and fructose, glycogen,starch, and cellulose.

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    Alternative Pathways Plants that fix carbon exclusively through the Calvin

    Cycle- C3 Plants

    Because of the 3-C compound PGA, that is initiallyformed.

    Example: Rice, wheat, oats, and soybeans

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    Alternative Pathways Plants in hot, dryclimates use alternative pathways

    Plant lose H2Oto the air-through small pores calledstomata (underside of leaf)

    Can be partially closed to prevent water loss

    Stomata are the major passageways through which CO2enters and O2leaves

    (when stomata are closed CO2levels decreaseand O2levels increase)

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    C4 Plants Fix CO4into 4-C

    compounds

    Partially close stomata

    during hottest part of theday

    Enzymes fix CO2into 4-Ccompounds and transportthem to cells where CO2isreleased and enters calvincycle ( lose as muchH2O as C3)

    Examples Corn, sugar cane, crabgrass

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    CAM Pathways Open stomata at night;close during the day

    Take in CO2at night and

    fix into compounds

    Release O2during theday and enter the Calvin

    cycle

    Example: cacti,pineapples