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MICROBIOLOGY SPECIAL SOURCE OF INFECTION Listeria- Refrigereted food (also Listeria only GPB with LPB causing Endotoxic Shock) Legionella-Water aerosol from airconditioner or cooler (History always speaks of a professor in a convention) P.multocida -Animal bite Clostridium - Canned food (botulinum toxin) Gastro enteritis within 6 hr - Preformed staphylococcal toxin Gastroenteritis within 6-8 hr with vomitting prominently - B. cereus ( H/O Rice Intake) Bloody Diarrhea 3-5 days after UNCOOKED Hamburger - E.Coli Leptospirosis - Rats, reservoir, Rainfall - 3Rs CULTURE MEDIAS1. Levinthal & Mannitol salt agar: Staphylococcus Aureus 2. Crystal Violet blood agar: Streptococcus pyogenes 3. Loeffler's serum slope & Potassium tellurite blood agar: Corynebacterium diphtheriae 4. Saboraud's dextrose agar: Nocardia 5. Robertson cooked meat medium: All Anaerobic bacteria 6. PLET medium: Bacillus Anthracis 7. Thayer martin or New york city medium: Gonococcus & Meningococcus 8. MacConkey's agar: All Enterobactericeae 9. Selenite F Broth & Tetrathionate broth: Salmonella and shigella 10. Bordet gengue medium: Bordetella pertussis 11. Castneda method: Brucella 12. Cetrimide or dettol agar: Pseudomonas 13. Lowenstein jensen (L-J medium) or dorset egg medium: Mycobacterium Tuberculosis 14. PPLO medium: Mycoplasma 15. skirrow's medium: H.pylori HYPERSENSITIVITY REACTIONS: A.C.I.D

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  • MICROBIOLOGY SPECIAL

    SOURCE OF INFECTION

    Listeria- Refrigereted food (also Listeria only GPB with LPB causing Endotoxic Shock)

    Legionella-Water aerosol from airconditioner or cooler (History always speaks of a professor in a convention)

    P.multocida -Animal bite

    Clostridium - Canned food (botulinum toxin)

    Gastro enteritis within 6 hr - Preformed staphylococcal toxin

    Gastroenteritis within 6-8 hr with vomitting prominently - B. cereus ( H/O Rice Intake)

    Bloody Diarrhea 3-5 days after UNCOOKED Hamburger - E.Coli

    Leptospirosis - Rats, reservoir, Rainfall - 3Rs

    CULTURE MEDIAS1. Levinthal & Mannitol salt agar: Staphylococcus Aureus

    2. Crystal Violet blood agar: Streptococcus pyogenes

    3. Loeffler's serum slope & Potassium tellurite blood agar: Corynebacterium diphtheriae

    4. Saboraud's dextrose agar: Nocardia

    5. Robertson cooked meat medium: All Anaerobic bacteria

    6. PLET medium: Bacillus Anthracis

    7. Thayer martin or New york city medium: Gonococcus & Meningococcus

    8. MacConkey's agar: All Enterobactericeae

    9. Selenite F Broth & Tetrathionate broth: Salmonella and shigella

    10. Bordet gengue medium: Bordetella pertussis

    11. Castneda method: Brucella

    12. Cetrimide or dettol agar: Pseudomonas

    13. Lowenstein jensen (L-J medium) or dorset egg medium: Mycobacterium Tuberculosis

    14. PPLO medium: Mycoplasma

    15. skirrow's medium: H.pylori

    HYPERSENSITIVITY REACTIONS:

    A.C.I.D

  • Type 1-Anaphylaxis A

    Type 2-Cytotoxic C

    Type 3-Immune complex mediated I

    Type 4-Delayed hypersensitivity D

    animals used 2 cultivate leprae

    m-mouse

    a-armadillos

    m-monkeys

    c-chimpanzee

    h-hamsters

    e-european hedgehog

    l-loris

    p-pangolin

    AEROBIC BACTERIA

    B.ANTHRACIS

    B.PERTUSSIS

    BRUCELLA

    KLEBSIELLA

    LISTERIA

    MYCOBACTERIA

    NOCARDIA

    NEISSERIA

    PSEUDOMNAS

    PROTEUS

    PASTEURELLA

    V.CHOLERAE

    Antibiotics and Ribosome Subunits

  • Buy AT 30, CELL at 50:

    Buy AT 30:

    A= aminoglycosides

    T= tetracycline

    CELL at 50:

    C= Chloramphenicol

    E= Erythromycin

    L=Linezolid

    L= cLindamycin

    BASH-Latent infections

    Latent infections are possible in:

    B-Brill zinser disease A-Ankylostoma, S-Slow virus infection, H-Herpes zoster

    Bile stained eggs

    TAsTe

    A = Ascaris lumbricoides

    T = Trichuris trichura

    T = Tenia species

    capsule seen in..

    Strep pneumonia

    Bacteroides

    H. influenza

    Anthrax (B. anthracis)

    E. coli

    Pasteurella

    Salmonella

    Menigitidis (N. Menigitidis)

    Yersinia pestis

  • Pseudomonas

    Francisella

    Brucella

    Klebsiella

    pneumoccus

    Bacteria that increase cAMP

    Increase cAMP: CAPE

    Cholera

    Anthracis

    Pertussis

    E.coli

    coagulase negative staph and novobiocin

    NOVOBIOCIN RESISTANCE IN SAPROPHYTICS (STAPHYLOCOCCUS SAPROPHYTICUS)

    THE OTHER COAGULASE NEGATIVE STAPH i.e. STAPH EPIDERMIDIS IS OPPOSITE of it =NOVOBIOCIN SENSITIVE

    dimorphic fungi :

    C:COCCCIDIODES

    A:ASPERGILOSSIS

    S:SPOROTRIX SHENCKII

    H:HISTOPLASMA

    B:BLASTOMYCES

    components of mc conkey:PLANT

    p=peptone

    l=lactose

    a=agar

    n=neutral red

    BFP-syphilis

    conditions in which BFP reaction occurs----- M THRIL

  • H-hepatitis

    I-infectious mononucleosis

    T-tropical eosinophilia

    L-leprosy(specially Lepromatous)

    R-relapsing fever

    M-malaria

    DNA viruses

    1)All DNA viruses r HHAPPPy viruses-

    Hepadna,

    Herpes,

    Adeno,

    Pox,

    Parvo,

    Papova.

    2)All r double stranded EXCEPT-Parvo(ie part of a virus-single stranded)

    3)All r linear EXCEPT -Papova & Hepadna(circular)

    4)All r icosahedral EXCEPT-Pox(complex)

    5)All replicate in nucleus EXCEPT-Pox(in cytoplasm as it carries its own DNA dependent RNA polymerase)

    6)Naked DNA viruses -PAP

    Parvo,Adeno,Papova

    7)HPH-enveloped viruses