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curriculum vitae

Personal Information

Name De Berardinis Piergiuseppe Address Via Bagni di Tritoli 5, 80070 Bacoli (NA), Italy

Tel +39 081 6132566 Cell: 339 8065835

E-mail p.deberardinis@ibp.cnr.it

Nationality Italian

Date of Birth Roma 4/ 11/ 1956

Gender M

Field of Interest Immunological studies for the formulation of new vaccines and innovative research products.

Professional Experience

2011-present Member of Faculty of the PhD course “Biomolecular Sceiences”, II° University of Naples, Italy.

2013-2015 Invited “Visiting scientist” at the “Universidade Federal do Rio de Janeiro” Brasil.

2008-2014 Member of the Board of Directors of the Italian society of Immunology, Clinical Immunology and Allergology (SIICA).

From 2001 “1° Ricercatore CNR” at the Institute of Protein Biochemistry, CNR, Naples, Italy.

1990-2001 Researcher and group leader at the Institute of Protein Biochemistry. 1985-1989 Post Doctoral research assistant at the Charing Cross Sunley Research

Centre, London, UK. 1983-1985 Fellow 1° Clinica Medica University“La Sapienza” Rome, Italy.

Function I° Ricercatore at IBP, CNR, Naples, Italy. Activity Tutor of students, PhD students and Post-Doc. PI and head of the

Laboratory of Immunology. Editor of: Immunology Letters, BMC Microbiology and other on-line journals.

Work place Institute of Protein Biochemistry, CNR, via P. Castellino 111, 80131 Naples, Italy.

Group Leader IBP Project New immunotherapeutic strategies (www.ibp.cnr.it)

1994 EMBO Fellowship, Istitute Pasteur, Paris, France. 1989 PhD Degree Univeristy of Londond, UK (examineers: Prof I Roitt and A

Boylston). 1987-1989 Wellcome Trust Foundation Fellowship. 1985-1987

1985 Fondazione Cenci-Bolognetti Fellowship.

Specialization in in Allergology and Clinical Immunology, University la Sapienza, Rome, Italy.

1981 MD (thesis honour), University “La Sapienza”, Rome, Italy.

Degrees Ph.D. in “Cellular Immunology; Specialization in Allergology and Clinical Immunology; MD.

Principal fields of professional interest

Medicine, Immunology.

Name of Institution for

Education

University “La Sapienza”, Rome (Italy); University of London (UK).

Level in National or International classification

Principal Investigator

Education

Personal Skills

Mother tongue Italiano

Other languages Self evaluation Understanding Speaking Writing

European standard Listening Reading Spoken Interaction

Spoken Production

English C2 C2 C2 C2 C2 French C1 C1 B2 B2 C1

Portuguese C1 C1 B2 B2 B2

Synthesis of the professional profile

MD specialized in Immunology, Dr De Berardinis has committed his scientific career to studying the molecular and cellular mechanisms of the immune response. In the recent years, he focused his interest in the attempt to translate the results of his researches into immunotherapeutic strategies and products. This was finalised in four patent applications on immunologic arguments. Of rilevance is the patent entitled “T cell receptors” concerning a technology aimed to be used in cancer adoptive T cell therapy, a topic which is collecting renewed interest by the pharmaceutical companies. Notwithstanding its initial state of development the patent was acquired by a private company which also invested in its development . Most recently, Dr De Berardinis filed a CNR patent entitled: ”Phages conjugates and their use “, which proposes the use of bacteriophages fd as a nanocarrier able to deliver antigenic determinants to human dendritic cells and induce sustained and protective immune responses. During his career Dr De Berardinis was entitled as PI of several national and international reserach grants. He has performed teaching activity and is in the faculty of the Ph.D. course “Biomolecular Sciences” of the second University of Naples. Dr De Berardinis is author of 59 publications on peer reviewed international journals and has an active impact factor (firts/last, corresponding author) of 215.77 (source Isi Wos). Dr De Berardinis is associate editor of two internationals journals (SJR quartile Q1 and Q2).

List of Pubblications

1. Costa V, Righelli D, Russo F, De Berardinis P, Angelini C, D’Apice L. (2017). Distinct Antigen Delivery Systems Induce Dendritic Cells’Divergent Transcriptional Response: New Insights from a Comparative and Reproducible Computational Analysis. Int J Mol Sci Feb 25,18(3). Pii:E494. doi: 10.3390/ijms 18030494.

2. Trovato M, Maurano F, D’Apice L, Costa V, Sartorius R, Cuccaro F, McBurney SP, Krebs SJ, Prisco A, Ciccodicola A, Rossi M, Haigwood NL, De Berardinis P. (2016). E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells. BMC Microbiol Jul 16;(16(1) 152. Doi: 10.1186/s12866-016-0772-x.

3. D’Apice L, Costa V, Sartorius R, Trovato M, Aprile M, De Berardinis P. (2015). Stimulation of innate and

adaptive immunity using filamentous bacteriophage fdtargeted to DEC-205. J Immunol Res 2015: 585078. doi: 10.1155/20157585078. Epub 2015 Aug 26. PMID: 26380324.

4. Trovato M, De Berardinis P. (2015). Novel antigen delivery systems. World J Virol Aug 12;4(3):156-68.

doi: 10.5501/wjv.v4.i3.156. PMID: 26279977.

5. Sartorius R, D’Apice L, Trovato M, Cuccaro F, Costa V, De Leo MG, Marzullo VM, Biondo C, D’Auria S, De Matteis MA, Ciccodicola A, De Berardinis P. (2015) Antigen delivery by filamentous bacteriophage fd displaying an anti-DEC-205 single-chain variable fragment confers adjuvanticity by triggering a TLR9-mediated immune response. Embo Molecular Medicine Apr 17;7(7):973-88 doi:10.15252/emmm.201404525 PMID: 25888235.

6. Krebs SJ, Mc Burney S, Kovarik DN, Smith C, Jaworsky JP, Sutton WF, Trovato M, Waagmeester G,

Barnett SJ, De Berardinis P, Haigwood N. (2014) Multimeric scaffolds displaying the IV-1 envelope Mper- specific antibodies and cross-neutralizing antibodies when co-immunized with gp 160. Plos One Dec 16;9(12):e113463. doi:10.1371/journal.pone0113463. eCollection 2014.

7. Mantile F, Trovato M, Santoni A, Barba P, Ottonello S, De Berardinis P, Prisco A(2014). Alum and

Squalene-Oil-in-Water Emulsion enhance the titer and avidity of Anti-Ab antibodies induced by Multimeric Protein Antigen (i-11)E2, preserving the IgG1-skewed isotype distribution. Plos One Jul 1;9(7):e101474, doi:10.1371/journal .pone 0101474.

8. D’Apice L, Costa V, Valente C, Trovato M, Pagani A, Manera S, Regolo L, Zambelli A, Ciccodicola A,

De Berardinis P.(2013) Analysis of SEMA6B gene expression in breast cancer: Identification of a new isoform. Biochim Biophys Acta May 9 doi:pii:S0304-4165(13)00199-2.

9. Castiglione F, Mantile F, De Berardinis P, and Prisco A. (2012). "How the interval between prime and

boost injection affects the immune response in a computational model of the immune system". Comput Math Methods Med 2012:842329. Epub 2012 Sep.

10. Prisco A, and De Berardinis P. (2012) Memory immune response: a major challenge in vaccination.

Biomolecular Concepts Published online 10/09/2012 : 479-486 doi: 10.1515/bmc-2012-0010.

11. Prisco A. and De Berardinis P. (2012). Filamentous bacteriophage fd as an antigen delivery system in vaccination. Int J Mol Sci 13,: 5179-5194, doi:10.3390/ijms13045179.

12. Schiavone M, Fiume G, Caivano A, de Laurentiis A, Falcone C, Masci F, Mimmi S, Palmieri C, Pisano A, Pontoriero M, Rossi A, Scialdone A, Vecchio E, Andreozzi C, Trovato M, Rafay J, Ferko B, Montefior D, Lombardi A, Morsica G, Poli G, Quinto I, Pavone V, De Berardinis P, Scala G. (2012).Design and characterization of a peptide mimotope of the HIV-1 gp120 Bridging sheet. Int J Mol Sci 13,: 5179-5194 doi:10.3390/ijms13055674.

13. Trovato M, Krebs SJ,Haigwood NL, De Berardinis P. (2012) Delivery strategies for novel vaccine

formulations. World Journal of Virology February 12(1): ISSN 2220-3249(online) doi:10-5501/wjv.v1.i1.1.

14. Jaworski JP , Krebs SJ, Trovato M, Kovarik DN, Brower Z, Sutton WF, Waagmeester G, Sartorius R,

D’Apice L, Caivano A, Doria-Rose NA, Malherbe D, Montefiori D, Barnett S, De Berardinis P, Haigwood NL (2012) Co-immunization with Multimeric Scaffolds and DNA Rapidly Induces Potent Autologous HIV-1 Neutralizing Antibodies and CD8+ T Cells. PlosOne 7(2):e31464, doi: 10.137/journal.pone0031464. PMID: 22606037.

15. Sartorius R, Bettua C, D'Apice L, Caivano A, Trovato M, Russo D, Zanoni I, Granucci F, Mascolo D,

Barba P, Del Pozzo G, and De Berardinis P. (2011) Vaccination with Filamentous Bacteriophages trageting DEC-205 induces DC maturation and potent anti-tumor T cell response in absence of adjuvants. Eur J Immunol Sep;41(9):2573-84.

16. Costa V, Angelini C, D'Apice L, Mutarelli M, Casamassimi A, Sommese L, Gallo MA., Aprile M, Esposito

R, Leone L, Donizetti A, Crispi S, Rienzo M, Sarubbi B, Calabrò R, Picardi M, Salvatore P, Infante T, De Berardinis P, Napoli C, Ciccodicola A (2011) Massive-scale RNA-Seq analysis of non ribosomal transcriptome in human trisomy 21. PlosOne Apr 20;6(4):e18493.

17. Mantile F, Basile C, Cicatiello V, De Falco D, Caivano A, De Berardinis P, Prisco A. (2011) A multimeric

immunogen for the induction of immune memory to beta amyloid. Immunol Cell Biol Jul;89(5) 604-9.

18. Caivano A, Doria-Rose NA, Buelow B, Sartorius R, Trovato M, D'Apioce L, Domingo GJ, Sutton WF, Haigwood NL, De Berardinis P. (2010) HIV-1 Gag p17 presented as virus like particles on the E2 scaffold from Geobacillus stearothermophilus induces sustained humoral and cellular immune responses in the absence of IFNg production by CD4+ T cells.Virology 2010 Nov 25;407(2) 296-305.

19. Del Pozzo G, Mascolo D, Sartorius R, Citro A, Barba P, D'Apice L, De Berardinis P. (2010) Triggering

DTH and CTL activity by fd filamentous bacteriophages: role of CD4+ T cells in memory responses. J Biomed Biotechnol 2010: 894971. Epub 2010 Apr 29.

20. Sartorius R, Pisu P, D’Apice L, Pizzella L, Romano C, Cortese G, Giorgina A, Santoni A, Velotti F., De

Berardinis P. (2008). The use of filamentous bacteriophage fd to deliver MAGE-A10 or MAGE-A3 HLA-A2 restricted peptides and to induce strong anti-tumor CTL responses. J of Immunol 180:3719-3728.

21. Mascolo D, Barba P, De Berardinis P, Di Rosa F, Del Pozzo G. (2007) Phage display of a CTL

epitope elicits a long-term in vivo cytotoxic response. FEMS Immunol Med Microbiol 14; 50(1):59-66.

22. D’Apice L, Sartorius R, Caivano A, Mascolo D, Del Pozzo G, Scotto Di Mase D, Ricca E, Li Pira G, Manca F, Malanga D, De Palma R, De Berardinis P. (2007) Comparative analysis of new innovative formulations based on the use of procaryotic display systems. Vaccine 25(11) : 1993-2000.

23. Li Pira G, Bottone L, Ivaldi F, Del Galdo F, Papa F, Accolla R, Koopman G, Abbate G, De Berardinis P, D’Apice L, De Palma R and Manca F. (2005). Human naive CD4 T-cell clones specific for HIV ENV persist for years in vivo in the absence of antigenic challenge. J Acquir Immune Defic Syndr Oct 1;40(2) : 132-139.

24. De Berardinis P. and Haigwood N. (2004). New recombinant vaccines based on the use of procaryotic

antigen-display systems. Exp.Rev.Vaccines 3(6): 673-679.

25. De Berardinis P, Sartorius R,Caivano A, Mascolo D, Domingo GJ, Del Pozzo G, Gaubin M, Perham RN, Piatier-Tonneau D. and Guardiola, J. (2003). Use of fusion protein and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection. Current HIV Research 1 (4): 441-446.

26. Sartorius R, D’Apice L, Barba P, Guardiola J, Santoni A, Velotti F, De Berardinis P. (2003) Induction

of human NK cell-mediated cytotoxicity by CD40 triggering of antigen presenting cells. Cellular Immunology 221/2 :81-88.

27. Domingo GJ, Caivano A, Sartorius R, Barba P, Backstrom M, Piatier-Tonneau D, Guardiola J, De

Berardinis P. and Perham RN. (2003). Induction of specific T-helper and cytolytic responses to epitopes displayed on a virus-like protein scaffold derived from the pyruvate dehydrogenase multienzyme complex. Vaccine 21: 1502-1509.

28. Gaubin M, Fanutti C, Durrbach A, Mishal Z, Durrbach A, De Berardinis P, Sartorius R, Del Pozzo G,

Guardiola J, Perham RN, Piatier-Tonneau D. (2003). Processing by APC of filamentous phage displaying antigenic epitopes targets to both HLA class I and class II peptide loading compartments. DNA Cell Biology 22(1):11-18.

29. Bes C, Briant-Longuet L. Cerruti M, De Berardinis P, Devauchelle G, Devaux C, Granier C, Chardes

T. (2001). Efficient CD4 binding and immunosuppressive properties of the 13B8.2 monoclonal antibody are displayed by its CDR-H1-derived peptide CB1. FEBS Lett 508(1):67-74.

30. Caivano A, D’Apice L, Tiberio C, Prisco A, Acuto O, Guardiola J, De Berardinis P. (2001). Design of

cassette vectors permitting cloning of all types of human TCR variable alpha and beta regions. J Immunol Methods 1;255:125-34.

31. Guardiola J, De Berardinis P, Sartorius R, Fanutti C, Perham RN, Del Pozzo G (2001). Phage display of

epitopes from HIV-1 elicits strong cytolytic responses in vitro and in vivo. Adv Exp Med Biol 495:291-8.

32. Del Pozzo G, Bonomi G, Moschella F, Barba P, De Berardinis P, Guardiola J. (2001). Recombinant antigens to establish a model of autoimmunity in mice. Transplant Proc. Feb-Mar:33(1-2):57

33. De Berardinis P, Sartorius R, Fanutti C, Perham RN, Del Pozzo G, Guardiola G. (2000) Phage display

of peptide epitopes from HIV-1 elicits strong cytolytic responses in vitro and in vivo. Nature Biotechnology 18(8): 873-876.

34. Bonomi G, Moschella F, Ombra MN, Del Pozzo G, Granier C, De Berardinis P. and Guardiola J.

(2000) Modulation of TCR recognition of MHC class II/peptide by processed remote n- and c-terminal epitope extensions. Human Immunology 61 (8): 753-763.

35. D'Apice L, De Berardinis P, Pasquinelli R, Capasso I, D’Aiuto M, D’Aiuto G, Anzisi AM, Favre R, Cermola M, Barba P, Guardiola J. (2000) Identification of a new subset of cells exhibiting dendritic phenotypes in patients affected by breast proliferative disorders. Human Immunology 61(8): 739-752.

36. Pasquinelli R, Barba P, Capasso I, D’Aiuto M, D’Aiuto G, Anzisi AM, De Berardinis P, and

Guardiola J. (1999) Circulating antibodies against the breats tumor marker GCDFP-15/gp17 in mammary carcinoma patients and in patientscarrying benign brast conditions. Int J Cancer 84(6): 568-572.

37. Prisco A, Bonomi G, Moschella F, Ombra MN, De Berardinis P, Guardiola J. (1999) In vitro

immunization with a recombinant antigen carrying the HIV-1 RT 248-262 determinant inserted at different locations results in altered TCR VB region usage. Human Immunol 60(9): 755-763.

38. Fenoglio D, Li Pira G, De Berardinis P, Saverino D, Terranova MP, Ombra MN, Bracci L, Lozzi L,

Viotti C, Guardiola J. and Manca F. (1999) Antagonistic activity of HIV-T helper peptides flanked by an unrelated carrier protein. Eur J Immunol 29(5): 1448-1455.

39. De Berardinis P, D’Apice L, Prisco A, Ombra MN, Barba P, Del Pozzo G, Petukhov S, Malik P,

Perham RN, Guardiola J. (1999) Recognition of HIV-derived B and T cell epitopes displayed on filamentous phages. Vaccine 17(11-12): 1434-1441.

40. Troncone R, Gianfrani C, Mazzarella G, Greco L, Guardiola J, Auricchio S, De Berardinis P. (1998) The

majority of gliadin specific T cell clones from the coeliac small intestinal mucosa produce both gInterferon and IL4. Digestive diseases and sciences 43(1): 156-161.

41. De Berardinis P , Guardiola J, Manca F. (1997) Epitope context and reshaping of activated T helper cell

repertoire. Human Immunology 54(2): 189-193.

42. Prisco A, Troncone R, Mazzarella G , Gianfrani C , Auricchio S, Even J, Tiberio C, J. Guardiola J and De Berardinis P. (1997) Identical TCR _ chain rearrangements are present in T cells infiltrating the jejunal mucosa of untreated coeliac patients. Human Immunol 55(1): 22-33.

43. Manca F, De Berardinis P, Fenoglio D, Ombra MN, Li Pira G, Saverino D, Autiero M, Lozzi L, Bracci

L, Guardiola J. (1996) : Antigenicity of HIV-derived T helper determinants in the context of carrier recombinant proteins : effect on T helper cell repertoire selection. Eur J Imunol. 26(10): 2461-2469.

44. De Berardinis P, Ombra MN, Buono C, Toraldo R, Vetrano F, and Guardiola J. (1994) Long-term culture

and T cell receptor analysis of T cell clones isolated from a patient with adenosine deaminase deficiency and type I diabetes. Clin Immunol Immunopathol 73(3): 362-366.

45. Nisticò P , De Berardinis P, Morrone S, Alonzi T, Buono C, Venturo I, Natali PG, (1994) Generation and

characterization of two human a/b T cell clones recognizing autologous breast tumor cells through a HLA- and TCR/CD3 independent pathway. J Clin Invest 94 (4) : 1426-1431.

46. De Berardinis P. (1991) T cell subsets and their lymphokines. Annali dell'Istituto Superiore di Sanità.

Immunology and Biotechnology 27 (1): 41-49

47. Londei M, Verhoef A, Hawrylowiczv C, Groves J, De Berardinis P, Feldmann M. (1989) Interleukin 7 (IL7) is a growth factor for human mature T cells. Eur J Immunol 20(2): 425-428.

48. Londei M, Verhoef A, De Berardinis P, Kissonerghis M, Grubeck Loebenstein B, Feldmann M, (1989) Definition of a population of CD4-8- T cells expressing ab T cell receptor which respond to IL2, IL3 and IL4. Proc Nat Acad Sci USA 86(21): 8502-8506.

49. De Berardinis P, Londei M, James RF, Lake SP, Wise PH, Feldmann M. (1989) CD4+ T cell damage to

islet b cells Lancet Feb 11;1(8633): 329.

50. De Berardinis P, Londei M, James RF, Lake SP, Wise PH, Feldmann M. (1988) Do CD4-positive cytotoxic T cells damage islet b cells in type 1 diabetes? Lancet, Oct 8;2(8615): 823-824.

51. De Berardinis P, Londei M, Kahan M, Balsano F, Kontiainen S, Bottazzo GF, Feldmann M. (1988) The

majority of the activated T cells in the blood of Insulin Dependent Diabetes Mellitus (IDDM) patients are CD4+. Clinical and Experimental Immunology, 73(2): 255—259.

52. Feldmann M, Londei M , Grubeck Loebenstein B, De Berardinis P, Leech Z, Turner M, Barrett K, Buchan

G, Maini RN (1988) Understanding what infiltrating cells do in autoimmunity : combination of cellular and molecular approaches. Transplantation Proceedings, 79(2) : 311-314.

53. De Berardinis P, Londei M, Carrel S, Feldmann M. (1988) Regulation of clonal growth by anti T cell

receptor antibody directed lysis. Immunology, 64(3):439-443.

54. Londei M, Grubeck Loebenstein B, De Berardinis P, Greenall C, Feldmann M. (1988) Efficient propagation and cloning of human T cells in the absence of antigen using OKT3, IL2 and antigen presenting cells. Scand J Immunol 27(1):35-46.

55. Bottazzo GF, Londei M, Bosi E, Mirakian R, Belfiore A, De Berardinis P, Todd I, Pujol-Borrel R, Feldmann

M. (1986) Inappropriate HLA classII expression on epithelial cells: new insight in the pathogenesis of distinctive autoimmunity. Ann Ital Med Int Suppl 1:20-23.

56. Feldmann M, Londei M, De Berardinis P, Essery G, Greenall C, Grubeck Loebenstein B, Knight J, Leech

Z, Kingsbury A, Maini RN (1986) Autoimmune disorders analysis by study of T cells, implications and speculations. In : Agents and Actions vol. 19(5-6): 252-255.

57. Brandimarte C, Valesini G, De Berardinis P, Palange P, Serra F, Balsano F. (1988) La policondrite

recidivante : un caso con anticorpi anti cartilagine , anti-cardiolipina e anti-centromero Rec Prog Med 79 (2) :67-69.

58. Valesini G, Pastore R, De Berardinis P, Serafini U, Vincent A, and Bottazzo GF (1983) Appearance of

anti-acetilcholine receptor antibodies coincident with onset of myasthenic weakness in patients with SLE. Lancet April 9; 1(8328): 831.

59. Pastore R, De Berardinis P, Ventura G, Minisola G, Valesini G (1983) Immune complexes and anti-

IgG activity in the sera and synovial fluid of patients with Juvenile Rheumatoid Arthritis. Folia Allergologica et Immunologica Clinica Vol.XXX n.1

Books

Phage displayed of epitopes from HIV-1 elicits strong cytolitic responses in vitro and in vivo Guardiola J, De Berardinis P., Sartorius R Fanutti C, Perham R Del Pozzo G Progress in basic and clinical immunology. Book series . Advances in Experimental Medicine and Biology 1996.ISBN 0-306-46656-2 . Editors Macklewicz A, Kurpisz M, Zeromski J

Immunogenicity of B and T Epitopes Displayed on Bacteriophage Fd. Antonella Prisco and Piergiuseppe De Berardonis. Immunogenicity ISBN: 978-1-61761-591-7. Editor: C.J. Villanueva © 2010 Nova Science Publishers, Inc.

Sartorius R., Russo D., D'Apice L. and De Berardinis P (2012) Filamentous bacteriophages fd: an antigen and gene delivery system. Innovation in Vaccinology. Springer ISBN 978-94-007-4542-1

Patents

1. Perham Richard, Fanutti Cristina, Guardiola John, De Berardinis Piergiuseppe, Piatier-Tonneau Dominique, Gaubin Muriel : “Recombinant double hybrid filamentous bacteriophage”. PCT/EP2000/009596. Publication date: 12/04/2001

2. Prisco Antonella, De Berardinis Piergiuseppe, De Falco Diana, Caivano Antonella: “Multimeric proteins

able to induce an antibody response against the beta-amyloid and use thereof”. NA2008A000006 (2011). European patent: n.2238160. Release date: 07/01/2016

3. Lucian D’Apice, Umbero Oreste, Piergiuseppe De Bereradinis: :”Varianti del recettore delle cellule T e

loro uso nella terapia tumorale”. RM2014A000632 (2014) International application “T cell receptors”:. PCT/EP2015/075607 (2015) ; Numero internazionale di pubblicazione n. WO2016/071343. Publication date: 12/05/2016

4. Rossella Sartorius, Piergiuseppe De Berardinis: “Coniugati fagici e loro usi”. Domanda n.102017000009270. Filing date: 27/01/2017