Curriculum Vitae - Home - Royan Institute tavallaei 95.pdf · 31. Zarei-Kheirabadi M, Shayegan Nia E, Tavalaee M, Deemeh MR, Arabi M, Forouzanfar M, Javadi GR, Nasr-Esfahani MH. Evaluation

1

Curriculum Vitae

1) Personal Information: Surname: TAVALAEE First name: MARZIYEH

Gender: Female

Nationality: Iranian

Date of birth: year: 1978 month: 7 day: 28 = Year: 1357 month: 5 day: 6

Place of Birth: Esfahan

Occupation: Ph.D

Academic member of the Royan Institute in Tehran, Iran

Address: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR,

Isfahan, Iran

P.O. Box: 19395-4644, Tehran, IRAN.

TEL: 0098 31 95015682

FAX: 0098 31 95015686

[email protected] ;[email protected] MAIL:-E

mailto:[email protected]

2

Number Activities

55 International Articles

23 National Research & Scientific Articles

5

1

2

Book publications: - Persian - Collaborated in writing English book

chapter - writing English book chapter

65 Conferences and Meetings

47 Projects

7 Prize

6 Presentation of Workshop

19 Consulting of thesis

2 Patent

3

2) Educational background:

Degree

Major

University

Date

B.Sc

Biology

Falavarjan Azad Uni. (Esfahan, Iran)

1997-2001

M.Sc

Animal Sciences Biology

Damghan Azad Uni. (Damghan, Iran)

2004-2006

Ph.D Developmental biology Tehran Azad Uni

Research Sciences 2013- 2016

(Tehran, Iran)

4

3) Book publications: 1- Sperm: Identification and selection of sperm from Sperm the biological and clinical aspect in ICSI patients (2010), is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN: 978-964-8115-83-3. Second edition

2- WHO laboratory manual for the Examination and processing of human semen, Translated by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee (ISBN: 978-600-6040-00-4). 3- Collaborated in writing a English book chapter: “Mechanism of human oocyte activation during ICSI and methodology of overcoming low or failed fertilization”. Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, 1 DOI 10.1007/978-1-4419-1780-5_1, © Springer Science+Business Media, LLC 2012. 4- Writing an English book chapter: “Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay”. is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. Spermatogenesis: Methods and Protocols, Methods in Molecular Biology, vol. 927, DOI 10.1007/978-1-62703-038-0_24, © Springer Science+Business Media, LLC 2013. 5- An English book chapter “Sperm selection based on surface electrical charge” is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. Non-Invasive Sperm Selection for In Vitro Fertilization: Novel Concepts and Methods. DOI 10.1007/978-1-4939-1411-1_4©Springer Science+Business Media, New York 2015. 6- In Vitro Fertilization (2013), is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN: 978-600-92587-9-6.

7- Intra-Cytoplasmic Sperm Injection (2014) by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN:978-600-94587-0-7.

8- Cellular and molecular aspects of reproduction biology (2015) by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN:978-600-94587-5-2.

5

6

4) Articles published in International Journals: 55

1. Panahi S, Fahami F, Deemeh MR, Tavalaee M, Gourabi H, Nasr-Esfahani MH. Chances to Have A Boy after Gender Selection

by Pre-Implantation Genetic Diagnosis Are Reduced in Couples with only Girls and without A Boy Sired by The Male Partner. Int J Fertil Steril. 2017 Jan-Mar; 10(4): 350-356.

2. Tavalaee M, Kiani – Esfahani A, Nasr-Esfahani MH. Relationship between potential sperm factors involved in oocyte activation

and sperm DNA fragmentation with ICSI clinical outcomes. CELL JOURNAL (Yakhteh) 2017 Jan-Mar; 18(4): 588-596.

3. Nasr-Esfahani MH, Deemeh.MR, Tavalaee.M, Sekhavati MH, Gourabi H. Zeta sperm selection improves pregnancy rate and

alters sex ratio in male factor infertility patients: a double-blind, randomized clinical trial. Int J Fertil Steril. 2016 Jul-Sep;

10(2):253-60.

4. Torki B, Tavalaee M, Bahadorani. M, Nasr-Esfahani MH. Selection of physiological sperm during ICSI. Andrologia. 2016 Apr;

1-6.

5. Modarres P, Tanhaei S, Tavalaee.M, Ghaedi. K, Deemeh.MR, Nasr-Esfahani.MH. Assessment of DPY19L2 deletion in familial

and non-familial individuals with globozoospermia and DPY19L2 genotyping. Int J Fertil Steril. 2016 Jul-Sep; 10(2):196-207.

6. Tavalaee M, Nasr-Esfahani MH. Expression profile of PLCζ, PAWP and TR-KIT in association with fertilization potential,

embryo development and pregnancy outcomes in globozoospermic candidates for ICSI and artificial oocyte activation.

Andrology. 2016 Sep; 4(5):850-6.

7. Kargar- Dastjerdy P, Tavalaee M, Salehi M, Falahati M, Izadi T, Nasr- Esfahani MH. Altered expression of KLC3 may affect

semen parameters. Int J Reprod Biomed (Yazd). 2016 Jan; 14(1):15-22.

7

8. Kamali-Dolat Abadi M, Tavalaee M, Shahverdi AH, Nasr-Esfahani MH. Evaluation of PLCζ and PAWP expression in

globozoospermic individuals. Cell J. 2016 Fall; 18(3):438-45.

9. Barekat F, Tavalaee M, Deemeh MR, Bahreinian M, Azadi L, Abbasi H, Rozbahani SH, Nasr-Esfahani MH. A Preliminary

Study: N-acetyl-L-cysteine Improves Semen Quality Following Varicocelectomy. Int J Fertil Steril. 2016 Apr-Jun; 10(1):120-6.

10. Javadian-Eliaderani S, Ghaedi K, Tavalaee M, Rabiee F, Deemeh MR, Nasr-Esfahani MH. Diagnosis of genetic defects through

parallel assessment of PLCζ and CAPZA3 in infertile men with history of failed oocyte activation. Iranian Journal of Basic

Medical Sciences. Iran J Basic Med Sci. 2016 Mar; 19(3):281-9.

11. Bahreinian M, Tavalaee M, Abbasi H, Kiani-Esfahani A, Shiravi AH, Nasr-Esfahani MH. DNA hypomethylation

predisposes sperm to DNA damage in individuals with varicocele. Syst Biol Reprod Med. 2015; 61(4):179-86.

12. Tavalaee M, Bahreinian M, Barekat F, Abbasi H, Nasr-Esfahani MH. Effect of varicocelectomy on sperm functional

characteristics and DNA methylation. Andrologia. 2015 Oct; 47(8):904-9.

13. Deemeh MR, Tavalaee M, Nasr-Esfahani MH. Health of Children Born Through Artificial Oocyte Activation: A Pilot Study.

Reprod Sci. Reprod Sci. 2015 Mar; 22(3):322-8.

14. Afiyani AA, Deemeh MR, Tavalaee M, Razi M, Bahadorani M, Shokrollahi B, Nasr-Esfahani MH. Evaluation of heat-shock

protein A2 (HSPA2) in male rats before and after varicocele induction. Mol Reprod Dev. 2014 Aug; 81(8):766-76.

15. Bateni Z, Azadi L, Tavalaee M, Kiani-Esfahani A, Fazilati M, Nasr-Esfahani MH. Addition of Tempol in semen

cryopreservation medium improves the post-thaw sperm function. Syst Biol Reprod Med. 2014 Aug; 60(4):245-50. Epub 2014

Apr 7.

16. Tavalaee M, Deemeh MR, Arbabian M, Kiyani A, Nasr-Esfahani MH. Relationship between fertilization rate and early

apoptosis in sperm population of infertile individuals. Andrologia. Andrologia. 2014 Feb; 46(1):36-41.

http://www.ncbi.nlm.nih.gov/pubmed/25747432

http://www.ncbi.nlm.nih.gov/pubmed/25747432

8

17. Hosseinpour E, Shahverdi A, Parivar K, Sedighi Gilani MA, Nasr-Esfahani MH, Salman Yazdi R, Sharbatoghli M, Tavalaee M,

Chehrazi M. Sperm ubiquitination and DNA fragmentation in men with occupational exposure and varicocele. Andrologia. 2014

May; 46(4):423-9. Epub 2013 Apr 17.

18. Charehjooy N, Najafi MH, Tavalaee M, Deemeh MR, Azadi L, Shiravi AH, Nasr-Esfahani MH. Selection of Sperm Based on

Hypo-Osmotic Swelling May Improve ICSI Outcome: A Preliminary Prospective Clinical Trial. Int J Fertil Steril. 2014 Apr;

8(1):21-8. Epub 2014 Mar 9.

19. Kiani-Esfahani A, Bahrami S, Tavalaee M, Deemeh MR, Mahjour AA, Nasr-Esfahani MH. Cytosolic and mitochondrial ROS:

which one is associated with poor chromatin remodeling? Syst Biol Reprod Med. 2013 Dec; 59(6):352-9. Epub 2013 Aug 22.

20. Bassiri F, Tavalaee M, Nasr Esfahani MH. Correlation between Different Patterns of Hypo-Osmotic Swelling and Sperm

Functional Tests. Int J Fertil Steril. 2013 Oct; 7(3):193-8. Epub 2013 Sep 18.

21. Zahedi A, Tavalaee M, Deemeh MR, Azadi L, Fazilati M, Nasr-Esfahani MH. Zeta potential vs apoptotic marker: which is more

suitable for ICSI sperm selection? J Assist Reprod Genet. 2013 Sep; 30(9):1181-6. Epub 2013 Jul 19.

22. Eskandari-Shahraki M, Tavalaee M, Deemeh MR, Jelodar GA, Nasr-Esfahani MH. Proper ubiquitination effect on the

fertilization outcome post-ICSI. Andrologia. 2013 Jun; 45(3):204-10. Epub 2012 Jul 1.

23. Forouzanfar M, Fekri Ershad S, Hosseini SM, Hajian M, Ostad-Hosseini S, Abid A, Tavalaee M, Shahverdi A, Vosough Dizaji

A, Nasr Esfahani MH. Can permeable super oxide dismutase mimetic agents improve the quality of frozen-thawed ram semen?

Cryobiology. 2013 Apr; 66(2):126-30. Epub 2012 Dec 28.

24. Motiei M, Tavalaee M, Rabiei F, Hajihosseini R, Nasr-Esfahani MH. Evaluation of HSPA2 in fertile and infertile individuals.

Andrologia. 2013 Feb; 45(1):66-72. Epub 2012 Jun 7.

25. Nasr Esfahani, Tavalaee M. Origin and Role of DNA Damage in Varicocele. Int J Fertil Steril. 2012 Oct; 6(3):141-6. Epub 2012

Dec 17.

9

26. Tavalaee M, Abbasi H, Deemeh MR, Fotohi F, Sadoughi Gilani MA, Nasr Esfahani MH. Semen parameters and chromatin

packaging in microsurgical varicocelectomy patients. Int J Fertil Steril. 2012 Oct; 6(3):165-74. Epub 2012 Dec 17.

27. Nasr-Esfahani MH, Deemeh MR, Tavalaee M. New era in sperm selection for ICSI. Int J Androl. 2012 Aug; 35(4):475-84. Epub

2011 Nov 15. Review.

28. Bassiri F, Tavalaee M, Shiravi AH, Mansouri S, Nasr-Esfahani MH. Is there an association between HOST grades and sperm

quality? Hum Reprod. 2012 Aug; 27(8):2277-84. Epub 2012 May 16.

29. Kiani-Esfahani A, Tavalaee M, Deemeh MR, Hamiditabar M, Nasr-Esfahani MH. DHR123: an alternative probe for assessment

of ROS in human spermatozoa. Syst Biol Reprod Med. 2012 Jun; 58(3):168-74. Epub 2012 Apr 30.

30. Navaeian-Kalat E, Deemeh MR, Tavalaee M, Abasi H, Modaresi M, Nasr-Esfahani MH. High total acrosin activity in varicocele

individuals. Andrologia. 2012 May; 44 Suppl 1:634-41. Epub 2011 Nov 2.

31. Zarei-Kheirabadi M, Shayegan Nia E, Tavalaee M, Deemeh MR, Arabi M, Forouzanfar M, Javadi GR, Nasr-Esfahani MH.

Evaluation of ubiquitin and annexin V in sperm population selected based on density gradient centrifugation and zeta potential

(DGC-Zeta). J Assist Reprod Genet. 2012 Apr; 29(4):365-71. Epub 2011 Dec 20.

32. Tavalaee M, Deemeh MR, Arbabian M, Nasr-Esfahani MH. Density gradient centrifugation before or after magnetic-activated

cell sorting: which technique is more useful for clinical sperm selection? J Assist Reprod Genet. 2012 Jan; 29(1):31-8. Epub 2011

Dec 15.

33. Aghajanpour S, Ghaedi K, Salamian A, Deemeh MR, Tavalaee M, Moshtaghian J, Parrington J, Nasr-Esfahani MH.

Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample. Hum Reprod.

2011 Nov; 26(11):2950-6. Epub 2011 Sep 6.

34. Fathi Z, Tavalaee M, Kiani A, Deemeh MR, Modaresi M, Nasr-Esfahani MH. Flow Cytometry: A Novel Approach for Indirect

Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies. Int J

Fertil Steril. 2011 Oct; 5(3):128-33. Epub 2011 Dec 22.

11

35. Azadi L, Abbasi H, Deemeh MR, Tavalaee M, Arbabian M, Pilevarian AA, Nasr-Esfahani MH. Zaditen (Ketotifen), as mast cell

blocker, improves sperm quality, chromatin integrity and pregnancy rate after varicocelectomy. Int J Androl. 2011 Oct; 34(5 Pt

1):446-52. Epub 2010 Sep 27.

36. Nasr-Esfahani MH, Tavalaee M, Arbabian M, Parrington J. Can Assessment of Total Acrosin Activity Help Predict Failed or

Low Fertilization Rate ICSI for Implementation of Artificial Oocyte Activation? The Open Andrology Journal, 2010; 2: 19-26

37. Soleimani M, Tavalaee M, Aboutorabi R, Adib M, Bahramian H, Janzamin E, Kiani A, Nasr-Esfahani MH. Evaluation of Fas

positive sperm and complement mediated lysis in subfertile individuals. J Assist Reprod Genet. 2010 Aug; 27(8):477-82. Epub

2010 Jun 15.

38. Nasr-Esfahani MH, Deemeh MR, Tavalaee M. Artificial oocyte activation and intracytoplasmic sperm injection. Fertil Steril.

2010 Jul; 94(2):520-6. Epub 2009 Apr 25. Review.

39. Deemeh MR, Tavalaee M, Ahamadi SM, Kalantari SAA, Alavi Nasab SV, Najafi MH, Nasr-Esfahani MH. The First Report of

Successfully Pregnancy After ICSI with Combined DGC/Zeta Sperm Selection Procedure in a Couple with Eleven Repeated Fail

IVF/ICSI Cycles. Int J Fertil Steril. 2010 Apr-Jun; 1(4):41-43.

40. Tavalaee M, Kiani A, Arbabian M, Deemeh MR, Nasr-Esfahani MH. Flow Cytometry: A New Approach for Indirect

Assessment of Sperm Protamine Deficiency. Int J Fertil Steril. 2010 Feb-Mar; 4(3):177-184.

41. Hatami-Baroogh L, Razavi S, Zarkesh-Esfahani H, Tavalaee M, Tanhaei S, Ghaedi K, Deemeh MR, Rabiee F, Nasr-Esfahani

MH. Evaluation of the leptin receptor in human spermatozoa. Reprod Biol Endocrinol. 2010 Feb;( 8):17.

42. Razavi SH, Nasr-Esfahani MH, Deemeh MR, Shayesteh M, Tavalaee M. Evaluation of zeta and HA-binding methods for

selection of spermatozoa with normal morphology, protamine content and DNA integrity. Andrologia. 2010 Feb; 42(1):13-9.

43. Nasr Esfahani MH, Abbasi H, Mirhosseini Z, Ghasemi N, Razavi S, Tavalaee M, Tanhaei S, Deemeh MR, Ghaedi K,

Zamansoltani F, Rajaei F. Can Altered Expression of HSPA2 in Varicocele Patients Lead to Abnormal Spermatogenesis? Int J

Fertil Steril. 2010 Oct-Dec; 3(4):104-113.

11

44. Babazadeh Z, Razavi S, Tavalaee M, Deemeh MR, Shahidi M, Nasr-Esfahani MH. Sperm DNA damage and its relation with

leukocyte DNA damage. Reprod Toxicol. 2010 Jan; 29(1):120-4. Epub 2009 Sep 15.

45. Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Mardani M, Moshtaghian J, Nasr-Esfahani MH. Selection of

sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod. 2009 Oct; 24(10):2409-

16. Epub 2009 Jun 24.

46. Tavalaee M, Razavi S, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology

outcome. Fertil Steril. 2009 Apr; 91(4):1119-26. Epub 2008 May 27.

47. Nasr-Esfahani MH, Abasi H, Razavi S, Ashrafi S, Tavalaee M. Varicocelectomy: semen parameters and protamine deficiency.

Int J Androl. 2009 Apr; 32(2):115-22. Epub 2007 Oct 30.

48. Khajavi NA, Razavi S, Mardani M, Tavalaee M, Deemeh MR, Nasr-Esfahani MH. Can Zeta sperm selection method, recover

sperm with higher DNA integrity compare to density gradient centrifugation? Iranian Journal of Reproductive Medicine. 2009;

7(2):73-77.

49. Nasr-Esfahani MH, Razavi S, Javdan Z, Tavalaee M. Artificial oocyte activation in severe teratozoospermia undergoing

intracytoplasmic sperm injection. Fertil Steril. 2008 Dec; 90(6):2231-7. Epub 2008 Jan 18.

50. Nasr-Esfahani MH, Razavi S, Vahdati AA, Fathi F, Tavalaee M. Evaluation of sperm selection procedure based on hyaluronic

acid binding ability on ICSI outcome. J Assist Reprod Genet. 2008 May; 25(5):197-203. Epub 2008 May 16.

51. Salamian A, Ghaedi K, Razavi S, Tavalaee M, Tanhaei S, Salahshouri I, Gourabi H, Nasr-Esfahani MH. Single Nucleotide

Polymorphism Analysis of Protamine Genes in Infertile Men. Int J Fertil Steril. May-Jun 2008; 2(1):13-18.

52. Nasr-Esfahani MH, Razavi S, Tavalaee M. Failed fertilization after ICSI and spermiogenic defects. Fertil Steril. 2008 Apr;

89(4):892-8. Epub 2007 Jun 21.

53. Tavalaee M, Nasr Esfahani MH, Deemeh MR. Etiology and Evaluation of Sperm Chromatin Anomalies. Int J Fertil Steril. 2008

May-Jun; 1(2):1-43.

12

54. Tavalaee M, Razavi S, Nasr-Esfahani MH. Effect of sperm acrosomal integrity and protamine deficiency on IVF and pregnancy

rate. Int J Fertil Steril. 2007; 1(1):27-34.

55. Deemeh MR, Tavalaee M, Razavi S, Nasr-Esfahani MH. Evaluation of protamine deficiency and DNA fragmentation in two

globozoospermia patients undergoing ICSI. Int J Fertil Steril. 2007; 1(2):85-88.

5) Articles published in National Research & Scientific Journals: 23

1. Navaeian-kalat E, Tavalaee M, Azadi L, Abbasi H, Deemeh MR, Arbabian M, Nasr-Esfahani MH. Known pathophysiological

mechanisms in the incidence of varicoceles. J Shahrekord Univ Med Sci. 2016; 18(1): 122-140

2. Barekat F, Tavalaee M, Azadi L, Nasr-Esfahani MH. Comparison of sperm parameters and its functional characteristics between fertile

and infertile men with varicocele. Cell and Tissue. 2016; 6(4): 523-532.

3. Tavalaee M, Parivar K, Javadian-Eliaderani S, Nasr-Esfahani MH. Centrosome and its role in gametogenesis and embryonic

development. J Shahrekord Univ Med Sci. 2016; 18(2): 143-169.

4. Tavalaee M, Parivar K, Nasr-Esfahani MH, Shahverdi AH, Ghaedi k. Comparison of chromatin structure and PLCζ in sperm from

infertile oligoasthenoteratozoospermic men and fertile individuals. J Shahrekord Univ Med Sci 2016. In press.

5. Aghajanpour S, Tavalaee M, Azadi L, Nasr-Esfahani MH. The role of phospholipase Czeta in male fertility as an oocyte activation

factor. J Shahrekord Univ Med Sci. 2015 Feb, Mar; 16(6): 19-26.

6. Shaygan nia E, Tavalaei M, Nasr- Esfahani MH. Role of ubiquitin-proteasome in spermatogenesis process. JIMS. 2014; 288(32):1-16.

13

7. Azadi L, Tavalaee M, Nasr-Esfahani MH. Introduction of routine and novel sperm selection methods for treatment infertile patient

undergoing ICSI procedure. J Kermanshah Univ Med Sci. 2014; 18(3): 181-196.

8. Deemeh MR, Tavalaee M, Nasr-Esfahani MH. Artificial Oocyte Activation: Methods and Clinical Efficiency after Intra-Cytoplasmic

Sperm Injection. JIMS, 2013 Aug; 240 (31): 851-866.

9. Motiei M, Tavalaee M, Nasr-Esfahani MH. The role and effect of HSPA2 in male infertility. Journal of Iranian Anatomical Sciences.

2012; 37(9):338-358. Review.

10. Navaeian-Kalat E, Tavalaee M, Abasi H, Nasr-Esfahani MH. Comparison of Sperm Parameters and DNA Integrity between Fertile and

Varicocele Individuals. Journal of Cell & Tissue (JCT). 2012; 3(2): 171-177.

11. Nasr-Esfahani MH, Razavi S, Mardani M, Khajavi N, Deemeh MR, Tavalaee M. The Comparison of Efficiency of Density Gradient

Centrifugation and Zeta Methods in Separation of Mature Sperm with Normal Chromatin Structure. Cell Journal. 2009; 11(2):168-175.

12. Deemeh MR, Nasr-Esfahani MH, Razavi S, Nazem HA, Moghadam S, Tavalaee M. The comparison of HA binding and Zeta methods

efficiency in selection of sperm with normal morphology and intact chromatin. JIMS. 2009; 27(92):46-56.

13. Taghadosi MT, Tavalaee M, Razavi S, Mardani M, Nasr Esfahani MH. Efficiency of Sperm Gradient Processing and HA-binding Test

for the Selection of Sperm with Normal Protamine, intact DNA and Normal Morphology. Cell Journal. 2008; 9(4):226-231.

14. Razavi S, Nasr-Esfahani MH, Tavalaee M, Ameri A. Assessing the sperm DNA damages on Assisted Reproductive Technology

outcome. Arak Medical University Journal. 2008; 11(1):21-31.

14

15. Nasr-Esfahani MH, Razavi S, Vahdati AA, Fathi F, Tavalaee M. Evaluation of sperm selection procedure based on its binding ability to

hyaluronic acide on ICSI outcome. JIMS. 2008; 26(89): 187-197.

16. Nasr-Esfahani MH, Tavalaee M, Deemeh MR. Origins and Evaluation of DNA Damage in Infertile Individual. Journal of Iranian

Anatomical Sciences. 2008; 6(24): 489-50.

17. Fathi F, Vahdati A, Nasr-Esfahani MH, Razavi S, Tavalaee M. Relation between sperm binding ability to hyaluronic acid with human

sperm parameters. Iranian biology Journal. 2008; 21(5):1-8.

18. Moghadam S, Nasr-Esfahani MH, Razavi S, Nazem HA, Deemeh MR, Tavalaee M .The efficiency of zeta method in separation of

sperm with normal morphology and chromatin structure. JSKUMS, 2008; 10(1):20-29.

19. Ashrafi S, Nasr-Esfahani MH, Razavi S, Abasi H, Molavi F, Tavalaee M. Effect of varicocelectomy on semen parameters and protamine

deficiency 3 and 6 months post-surgery. JIMS, 2007; 87(25):77-88.

20. Razavi S, Fathi F, Tavalaee M, Vahdati A, Nasr Esfahani MH. Relation between sperm binding ability to hyaluronic acid with

protamine deficiency and DNA fragmentation. Journal of Iranian Anatomical Sciences. 2007; 5(18):49-59.

21. Tavalaee M, Razavi S, Nasr-Esfahani MH. Effects of Spermiogenic Defects on Fertilization and Pregnancy Rate in IVF Patients. Cell

Journal. 2007; 9(2):103-110.

22. Razavi S, Nasr-Esfahani MH, Tavalaee M, Vaezi GH, Shiravi A. Relationship between protamine deficiency and acrosin activity with

fertilization rate post ICSI. Journal of Iranian Anatomical Sciences, 2006; 3(4):245-252.

23. Nasr Esfahani MH, Razavi S, Vaezi GH, Shiravi E, Tavalaee M. Effects of acrosomal activity and morphology on fertilization rates

following ICSI. Journal of reproduction and infertility. 2006; 7(3): 217-224.

15

6) Conferences and Meetings:65

NO

TITLE

AUTHOR

LOCATION

PRES.

DATE

1

The role of sperm markers involved

in oocyte activation in infertile men

with previous failed fertilization

Tavalaee M

1st National

Symposium of sperm

biology

speaker 2016

2

Novel sperm selection procedures in

ICSI Tavalaee M

2st workshop novel

and routine normal

sperm selection for

infertile men

procedures

speaker 2016

3

Comparison of two novel sperm

selection procedures: Zeta potential

vs MACS

Tavalaee M, Nasr Esfahani

The 16th Royan

International Congress

on Reproductive

Biomedicine

Poster 2015

4

Artificial Oocyte Activation Alone

Intra Cytoplasmic Sperm Injection in

Norozi, Tavalaee M,

Bahadorani, Nasr Esfahani

The 16th Royan

International Congress Poster

2015

16

Men with Severe Semen Parameters on Reproductive

Biomedicine

4

Efficiency of Magnetic-Activated

Cell Sorting System in Separation of

Normal Sperm

Ziarati, Tavalaee M,

Bahadorani, Nasr Esfahani

The 16th Royan


on Reproductive

Biomedicine

Poster 2015

5

Effect of Calcium Ionophore A23187

on Sperm Hyper-Active Motility

Patterns

Torki, Bahadorani,

Tavalaee M , Nasr Esfahani The 16th Royan


on Reproductive

Biomedicine

Poster 2015

6

Evaluation of quantitative expression

of PLCζ, PAWP

and tr-kit as prediction factors in

successful fertilization

after ICSI combined AOA

Tavalaee M, Nasr-Esfahani International congress on

reproduction (ISERB) Panel 2015

7

Efficiency of Magnetic-Activated

Cell Sorting for Sperm

Preparation before and after Density

Gradient Centrifugation.

Tavalaee M, Deemeh,

Arbabian,

Nasr-Esfahani

International congress on

reproduction (ISERB) speaker 2015

8

Study of Hyper-Activation Pattern of

Motility and Acrosome Reaction

after Density Gradient Centrifugation

Torki, Bahadorani

Tavalaee M, Nasr-Esfahani


reproduction (ISERB) speaker 2015

9

Cellular, Molecular and Clinical

perspective of Varicocele

Navaeian-kalat, Tavalaee

M, Abasi, Nasr-esfahani


reproduction (ISERB) Poster 2015

10 Evaluation sperm DNA damage and

its influence on infertility

Tavalaee M 1st National Symposium of the

Embryology Updates on the Research and

Oral 2014

17

Therapy (Workshop)

11

Assessment of sperm chromatin and morphology in different patterns of HOST and clinical outcome after ICSI

Basiri F, Tavalaee M, Nasr-

Esfahani MH

1st National

Symposium of the Embryology Updates on the Research and

Therapy

Oral

2014

12

Evaluation of human sperm acrosome reaction in the PVP condition

Torki B, Tavalaee M, Azadi L, Bahadorani M, Nasr-

Esfahani MH

1st National Symposium of the


Therapy

Poster

2014

13

Assessment of HSPA2 before and after varicocele induction in rat sperm

Atrian A, Deemeh MR, Tavalaee M, Nasr-Esfahani

MH



Therapy

Poster

2014

14

Effect of Tempol and quercitin on human sperm and viability during cryopreservation

Azadi L, Tavalaee M, Deemeh MR, Arbabian M,

Nasr-Esfahani MH



Therapy

Poster

2014

15

Comparison of sperm quality, oxidative stress, DNA fragmentation, protamine deficiency, and DNA methylation in varicocele and fertile

Bahreinian M, Tavalaee M, Nasr-Esfahani MH

15th Congress on

Royan International

Poster

2014

18

individuals

16

Effect of Centrifugation on Levels of Stress Oxidative of Human Spermatozoa

Azadi L, Tavalaee M,

Deemeh MR, Arbabian M, Nasr-Esfahani MH

15th Congress on

Royan International

Poster

2014

17

Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging, Miscarriage and Pregnancy Rate in Infertile Men With Varicocele

Tavalaee M, Deemeh MR ,

Nasr Esfahani MH

15th Congress on

Royan International

Poster

2014

18

Evaluation of heat shock protein A2 (HSPA2) in male rats before and after varicocele induction

Atrian Afiyani A, Deemeh MR, Tavalaee M, Nasr-

Esfahani MH

15th Congress on

Royan International

Poster

2014

19

Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging, Miscarriage and Pregnancy Rate in Infertile Men With Varicocele

Tavalaee M

15th Congress on

Royan International

Poster

2014

20

Comparison of human sperm capacitation status using the measured hyper-activation motility pattern between the DGC and DGC-PVP procedures

Torki Boldaji B, Azadi L,

Tavalaee M, Bahadorani M, Nasr-Esfahani MH

20th National Congress of

Reproductive Medicine

Poster

2014

21

Comparison of Zaditen and NAC on

Deemeh MR, Azadi L,

20th National

Poster

2014

19

semen parameters of individuals with varicocele after varicocelectomy

Arbabian M, Tavalaee M, Abbasi H, Nasr Esfahani MH

Congress of Reproductive

Medicine

22

Evaluation of sperm chromatin structure and morphology in different patterns of HOST positive sperm

Bassiri F, Tavalaee M, Nasr-

Esfahani MH



Poster

2014

23

Evaluation of Tempol effects on semen quality during human sperm cryopreservation

Azadi L, Deemeh MR,

Arbabian M, Tavalaee M



Poster

2014

24

Assessment of sperm parameters

and chromatin status in rat experimental varicocele

Atrian Afiyani A, Azadi L, Deemeh MR, Tavalaee M,

Nasr-Esfahani MH



Poster

2014

25

KLC3-gene expression comparison between fertile and infertile individuals

Kargar Dastjerdy P, Azadi L, Deemeh MR, Tavalaee M,

Salehi M, Falahaty M, Nasr-Esfahani MH



Poster

2014

26

Association between fertilization rate and externilization of phosphatidyl serine

Tavalaee M, Deemeh MR, Kiyani MH, Nasr-Esfahani

MH

14th Congress on Royan International

Poster 2013

21

27

Which novel sperm selection is suitable for ICSI? MACS-DGC or DGC-MACS

Tavalaee M, Deemeh MR, Nasr-Esfahani MH


Poster

2013

28

Comparison of sperm Acrosin Activity and DNA fragmentation between varicocele and infertile individuals

Navaeian Kalat E, Tavalaee M, Abbasi H, Nasr-Esfahani

MH


Poster 2013

29

Varicocele improved semen quality and chromatin integrity in normozoospermic and non-normozoospermic varicocele individual

Deemeh MR, Tavalaee M, Nasr- Esfahani MH


Poster 2013

30

HOST patterns and sperm quality

Bassiri F, Tavalaee M, Nasr-Esfahani MH


Poster 2013

31

Sperm selection based on surface charge: improvement of embryo quality in teratozoospermic individuals

Deemeh MR, Tavalaee M, Azadi L, Nasr-Esfahani MH


Poster 2013

32

Effect of NAC after varicocelectomy on sperm quality of varicocele individuals

Barekat F, Tavalaee M, Rozbahani SH, Nasr-

Esfahani MH,


Poster 2013

33

Comparison of semen parameters,

Barekat F, Deemeh MR,

14th Congress on

21

sperm DNA integrity and oxidative stress between fertile and varicocele

Tavalaee M, Nasr-Esfahani MH

Royan International Poster

2013

34

Comparison of sperm quality, oxidative stress, DNA fragmentation, protamine deficiency, and DNA methylation in varicocele and fertile individuals

Bahrainian M, Tavalaee M, Kiani A, Shiravi AH, Nasr-

Esfahani MH


Poster 2013

35

Pregnancy rate after varicocelectomy: Long-term outcome in a large number of varicocele individuals

Azadi L, Tavalaee M,

Deemeh MR, Abbasi H, Nasr-Esfahani MH


Poster

2013

36

Evaluation of heat shock protein A2 (HSPA2) in male rats before and after varicocele induction

Atrian Afiyani A, Deemeh MR, Bahadorani M,

Tavalaee M, Shokrollahi B, Nasr-Esfahani MH


Poster 2013

37

Assessment of sperm chromatin status in rat experimental varicocele

Atrian Afiyani A, Deemeh MR, Bahadorani M, Mazdak

M, Tavalaee M, Nasr-Esfahani MH


Poster 2013

22

38

Quantitative assessment of human sperm transcripts as genetic fingerprints of male infertility

Nasr-Esfahani MH, Tavalaee M, Tanhaee S, Ghaedi K, Deemeh MR,

Javadian S, Aghajanpour S, Salamian A

4th congress of the Asia Pacific Initiative

on Reproduction (ASPIRE)

Oral 2012

39

Evaluation of HSPA2 in fertile and infertile individuals

Motiei M, Tavalaee M, Rabiei F, Hajihosseini R,

Nasr-Esfahani MH


Poster 2012

40

Which Novel Sperm method is Functional? MACS-DGC or DGC-Zeta

Zahedi yeganeh A, Tavalaee M, Fazilati,. Nasr-Esfahani

MH


Poster 2012

41

Direct Evaluation of HspA2 in fertile and infertile individuals

Motiei M, Tavalaee M, Nasr-Esfahani MH


Poster

2011

42

Phospholipase C Zeta: as an Index to Assess Fertilization Potential of a Semen Sample

Aghajanpour S, Ghaedi K, Salamian A, Tavalaee M,

Deemeh MR, Nasr-Esfahani MH

12th Congress on

Royan International

Poster

2011

23

43

Zaditen (Ketotifen), as mast cell blocker, improves sperm quality, chromatin integrity and pregnancy rate after varicocelectomy

Azadi L, Tavalaee M, Deemeh MR, Nasr-Esfahani

MH

12th Congress on

Royan International

Poster

2011

44

Evaluation of sperm acrosin activity and chromatin structure in varicocele patients before and after varicocelectomy

Navaeian Kalat E, Tavalaee M, Deemeh MR, Nasr-

Esfahani MH


Poster

2011

45

Assessment of Apoptosis Marker in Novel Sperm Selection Procedure

Zarei M, Tavalaee M,

Deemeh MR, Nasr-Esfahani MH


Poster

2011

46

Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies

Fathi Z, Tavalaee M, Deemeh MR, Nasr-Esfahani

MH

12th Congress on Royan

International

Poster

2011

47

DHR 123: A suitable fluorescent mitochondrial probe for assessment of H2O2 in human spermatozoa

Kiani-Esfahani A, Tavalaee M, Deemeh MR,

Hamiditabar M, Nasr Esfahani MH

12th Congress on Royan

International

Poster

2011

48

Evaluation sperm DNA damage and its influence on infertility

Tavalaee M 11th Congress on Royan International

Oral 2010

24

49

Influence of sperm chromatin anomalies on ART outcome

Tavalaee M 9 th Congress on Reproductive Biomedicine

Oral

2008

50

The comparison of density gradiant centrifugation and Zeta methods in separation of mature and normal spermatozoa by TUNEL, SCD, AO and CMA3

Khajavi, Razavi S, Tavalaee M, Deemeh MR, Nasr-

Esfahani MH

9 th Congress on Reproductive Biomedicine

Oral

2008

51

Zeta method: a novel sperm selection method for ICSI with normal chromatin structure

Kheirollahi M, Nasr-Esfahani MH, Razavi S,

Moshtaghian J, Tavalaee M, Deemeh MR


Poster 2008

52

Relationship between expression levels of HSPA2 gene and chromatin structure in patients with varicocele

Mir Hosseini Z, Nasr-Isfahani MH, Razavi S, Rezazade H, Tanhaei S,

Deemeh MR, Ghasemi N, Tavalaee M


Poster 2008

53

Existence of leptin’s receptor (Ob-R) in spermatozoa from infertile males

Hatami.L, Nasr-Esfahani. MH, Razavi S, Zarkesh H, Deemeh MR, Tavalaee M


Poster 2008

54

Comparison between HA binding and Zeta method to select mature sperm for ICSI

Deemeh M, Nasr-Esfahani. MH, Razavi S, Nazem H, Shayeste Moghadam M,

Tavalaee M


Poster 2008

25

55

Relationship between protamine deficiency and acrosomal structure with pregnancy rate post IVF

Tavalaee M, Razavi S, Nasr-Esfahani MH

13 th Congress Of Iranian society for

reproductive medicine

Poster

2007

56

Sperm selection with normal morphology and chromatin using HA-binding

Taghadosi MT, Nasr-Esfahani MH, Razavi S,

Mardani M, Tavalaee M



Oral

2007

57

Effect of Ionomycin as oocyte activation factor in oligoasthenoteratozoospermic patients post ICSI

Razavi S, Javdan Z, Nasr-Esfahani MH, Tavalaee M



Oral

2007

58

Relationship between protamine deficiency and acrosin activity with fertilization rate post ICSI.

Tavalaee M, Razavi S, Nasr-Esfahani MH



Oral

2007

59

Effect of varicocelectomy on protamine deficiency 3 and 6 months post surgery.

Ashrafi, Nasr-Esfahani MH, Razavi S, Tavalaee M



Oral

2007

60

Relationship between banded sperm to acid hyaluronic with DNA fragmentation and sperm parameters.

Fathi Z, Nasr-Esfahani MH, Razavi S, Vahdati A,

Tavalaee M



Oral

2007

62

Comparison of sperm parameters before and after varicocelectomy and its effect on fertility.

Ashrafi, Nasr-Esfahani MH, Razavi S, Tavalaee M



Oral

2007

26

8) Projects: 47

No

TITLE

Director/ colleague

DATE

Grant

Evaluation of PLCζ & PAWP genes expression levels in infertile individuals sperm with globozoospermia

Director 2015 Royan Institute

Comparison of clinical outcomes in two sperm selection procedures (DGC and MACS-DGC) in infertile men ICSI candidate


Comparison of Clinical Outcome of Ionomycin and Strontium Chloride Treated Oocytes in Infertile ICSI Candidate Individuals.


Comparison of different sperm selection procedures to select normal Director 2015 Royan Institute

63

Effects of acrosomal activity and morphology on fertilization rates

following ICSI

Nasr Esfahani MH, Rezaei S, Tavalaee M.Vaezi G, shiravi

E

2 th

iranbioconference

Poster

2006

64

Relationship between protamine deficiency and acrosin activity with

fertilization rate post ICSI

Razavi S, Nasr-Esfahani MH, Tavalaee M,Vaezi G, Shiravi

E

2 th

iranbioconference

Poster

2006

65

SNP analyses of protamine genes in infertile men population.

Salamyian A, Nasr-Isfahani MH, Ghaedi K, Razavi S, Tanhaei S, Tavalaee M

10th Iranian Genetics Congress,

Tehran, Iran

Poster 2008

27

sperm for infertile individuals candidate for ICSI

1 Evaluation of DNA damage in different types of human abnormal sperm head morphology

Director

2014

Royan Institute

2 Comparison effect of Tempol and Quercetin on efficiency of human sperm freeze solution

Director

2014

Royan Institute

3 Comparison of clinical outcomes in two sperm selection procedures (DGC and MACS-DGC) in infertile men ICSI candidate

colleague

2014

Royan Institute

4 Comparison of clinical outcomes of activated oocyte with ionomycin and strontium chloride in infertile men candidate ICSI (In process)

colleague 2014 Royan Institute

5 Study of relationship between the KLC3 gene transcription rate with different sperm parameters and comparision of its transcription rate between fertile and infertile Individuals (Asthenozoospermia, Oligozoospermia and Teratozoospermia) (In process)

colleague

2014

Royan Institute

6 Evaluation of PLCζ & PAWP genes expression levels in infertile individuals sperm with globozoospermia (In process)

colleague

2014

Royan Institute

7 Comparison of human sperm Capacitation and Acrosome reaction status after density gradient centrifugation (DGC) and DGC-PVP procedures

Director

2014

Royan Institute

8

Evaluation and comparison of PAWP and PLC zeta gene expression in infertile with high fertilization and infertile with failed fertilization individuals ICSI candidate

colleague

2013

Royan Institute

9 Assessment of the ratio of sperm sex chromosome X to Y in samples of semen, colleague Royan Institute

28

DGC (Density Gradient Centrifugation) and Zeta sperm selection methods by means of Real-Time PCR.

2012

10 The effect of N-Acetylcysteine on sperm parameters, sperm chromatin and oxidative stress in infertile men after varicocelectomy:a double-blined controlled randomized

colleague

2012

Royan Institute

11 Assessment of Antioxidant Effect of MnTBAP in the freezing media on spermatic parameters, membrane integrity and sperm capacitation of ran spermatozoa after freezing thawing

colleague

2012

Royan Institute

12 Monitoring of babies born after ICSI and artificial oocyte activation with Ionomycin

colleague

2011

Royan Institute

13 Evaluation of PLC zeta gene expression and correlation its with acrosin activity enzyme and deficiency protamine in ICSI candidate patients

colleague

2011

Royan Institute

14 Evaluation and comparison of nuclear maturation apoptosis and sperm morphology in selected sperm from different form of HOS positive in infertile individuals

colleague

2011

Royan Institute

15 Study of correlation between fertilization rate and early apoptosis in sperm population of infertile individuals candidate for ICSI

Director

2011

Royan Institute

16 Quantitative evaluation of heat shock protein A2 (HSPA2) and sperm chromatin integrity in male rates before and after varicocele induction

colleague

2011

Royan Institute

17 Evaluation of Tempol supplementation on efficiency of human sperm freezing medium

colleague

2011

Royan Institute

29

18 Comparison of ICSI outcomes during two sperm selection procedures: routine and HOST

colleague

2011

Royan Institute

19 Study and comparison of sperm oxidative stress, DNA damage and DNA methylation in varicocele individuals before and after varicocelectomy

colleague

2011

Royan Institute

20 Comparison of efficiency of two method novel sperm selection: DGC-Zeta and MACS-DGC in infertile individulals

colleague

2011

Royan Institute

21 Comparison of different sperm selection procedures to select normal sperm for infertile individuals candidate for ICSI

Director

2010

Royan Institute

22 Study and comparison of heat shock protein A2 ( HspA2) in fertile and infertile males

colleague

2010

Royan Institute

23 Correlation between percentage of sperm ubiquitinated and protamine deficiency with fertilization rate in ICSI candidate patients

colleague 2010

Royan Institute

24 Comparison on the efficiency of DGC(Density Gradient Centrifugation) and DGC- Zeta methods for selection of human spermatozoa with low ubiquitination

colleague

2010

Royan Institute

25 The comparison of efficiency of DGC and DGC-Zeta for selection of sperm with low apoptosis

colleague

2010

Royan Institute

26 Evaluation and comparison of CAPZA3 gene expression in fertile and infertile individual’s sperm and it’s correlation with PLC expression and fertilization rate

colleague

2010

Royan Institute

27 Evaluation of sperm acrosin activity and chromatin structure in varicocele patients before and after varicocelectomy.

colleague Royan Institute

31

2010

28 Evaluation of relation between acrosin activity with artificial activation of oocyte in ICSI patients.

colleague

2009

Royan Institute

29 Comparison of efficiency of density gradient centrifugation (DGC) with combined DGC/Zeta method on ICSI outcome

colleague

2009

Royan Institute

30 Flow cytometry: a new approach for indirect assessment of protamine deficiency

colleague

2009

Royan Institute

31 Evaluation of effect of presence and absence and of basal bodies in the results of sperm protamine seficiency in semen sample of infertile individuals by using flow cytometry technique

colleague

2009

Royan Institute

32 Evaluation of correlation between the percentage of ROS and CMA3 and apoptosis positive sperm in infertile men by flow cytometry

colleague

2009

Royan Institute

33 Comparison of sperm morphology, DNA fragmentation, protamine deficiency and HSPA2 expression before, three and six month after varicocelectomy.


34 Single nucleotides polymorphism Analysis of Protamine Genes in Infertile Men Population.


35 The study of relationship between epigenetic changes and semen parameters, DNA fragmentation, protamine deficiency in ICSI and IVF patients


36 The comparison of efficiency density gradient centrifugation and Zeta methods in separation of mature sperm with normal Protamine and DNA by flow cytometry.


31

37 Evaluation of leptin receptor expression in fertile and infertile men



38 The study of efficiency of HA test and Zeta method in separation of mature sperm



39 The study of relationship between lymphocyte and sperm DNA fragmentation in infertile patient.


40 Evaluation the effect of zadetin (Ketotifen) as mast cell blocker on sperm parameters and sperm chromatin in infertile men before and after varicocelectomy


41 Study of sperm functional testes


42 Sperm selection based on its binding ability to hyaluronic acid for intra cytoplasmic sperm injection.

colleague 2007

Royan Institute

43 The effect of varicocelectomy on recovery of sperm morphology and protamine deficiency at 3 and 6 months post surgery.

colleague 2007

Royan Institute

44 The Study of relation between protamine deficiency and expression of HSPA2 in varicocele patients, before and after of varicocelectomy

colleague 2007

Royan Institute

45 Study of relationship between protamin deficiency with acrosin activity ,sperm normal morphology and fertilization rate in candidat of ICSI infertile couples

colleague 2006

Royan Institute

32

46 Effect of Ionomycin as oocyte activation factor in oligoasthenoteratozoospermic patients post ICSI

colleague

2006

Royan Institute

47 Effect of sperm chromatin structure on fertility colleague

2004 Royan Institute

9) Prize: 5

No

TITLE

AUTHOR

STATUS

DATE

Evaluation of sperm chromatin structure on fertility Tavalaee, Nasr-Esfahani

The Winner of Hamandishi research festival on medical sciences

2007

Evaluation of sperm chromatin structure on fertility Tavalaee, Nasr-Esfahani

Javan Kharazmi research festival on medical

sciences

2008

As excellent researcher in Isfahan city Tavalaee 5th

Paghoheshi va fan avari festival 2009

As excellent researcher in Royan Tavalaee Royan Institute 2012

As excellent researcher Tavalaee 2014 لیل از پژيهشگران ي فىايران برتر کشًرپاوسدهمیه جشىًارٌ تج

As excellent researcher in Isfahan city Tavalaee 10th

Paghoheshi va fan avari festival 2014

As excellent researcher Tavalaee The first International ISERB award 2015

33

10) Presentation of Workshop:

3nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of infertile individuals. 2014; Royan Institute

(Isfahan campus).

2nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of infertile individuals. 2007; Royan Institute (Isfahan campus). 3nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of infertile individuals. 2009; Royan Institute (Isfahan campus).

1 nd 3nd Royan Workshop on: spermogram and its interpretation in ART. 2010. Royan Institute (Isfahan campus). First Royan workshop on: novel and routine sperm selection procedures for infertile men. 2015. Royan Institute (Isfahan campus). 2nd Royan Workshop on: novel and routine sperm selection procedures for infertile men. 2016. Royan Institute (Isfahan campus).

11) Consulting of thesis: 19

1. Moloud Rashidi. Evaluation and comparison of three DNA methyltranferases gene expression between individuals with varicocele and

fertile men. 2016

2. Elahe.Raji. Comparative expression level miR-373 in the sperm of infertile Individuals patients referred to Center Fertility Infertility

Esfahan. Islamic Azad University, Shahrekord Branch. 2015.

34

3. Marziyeh Norozi Hafshejani. Comparison of Clinical Outcome Treated Oocyte whit Ionomycin and Strontium Chloride in Infertile ICSI

Candidate Individuals. Islamic Azad University Falavarjan Branch. 2015.

4. Niloofar ziarati. Comparison of Clinical Outcomes in two Sperm Selection Procedures, Routine (DGC) and Novel (MACS-DGC), in Infertile

Men ICSI Candidate. Islamic Azad University Falavarjan Branch. 2015.

5. Bahareh Torki. Study and comparison of human sperm motility patterns and acrosome reaction in the presence of PVP and density

gradient centrifugation method (DGC). Falavarjan Branch, Islamic azad University 2014.

6. Zahra Bateni. Evaluation of Tempol supplementation on efficiency of human sperm freezing medium. Payame Noor University

(Esfahan) 2013.

7. Aliyeh Zahedi Yeganeh. Comparison of efficiency of two method noval sperm selection (DGC-ZETA) and (MACS-DGC) in infertile

individuals. Payame Noor University (Esfahan) 2013.

8. Forogh Barekat. Evaluation of the effect N-Acetylcysteine (NAC) on sperm parameters, sperm chromatin and oxidative stress in infertile

men after varicocelectomy. Falavarjan Branch, Islamic azad University 2013.

9. Mahsa bahreinian. Evaluation and comparison of stress oxidative, DNA Fragmentation, DNA methylation sperm in varicocele individual

before and after of varicocelectomy. Damghan branch, Islamic azad University 2012.

10. Nasim Charehjooy. Comparison of ICSI outcomes during two sperm selection procedures: routine and HOST. Damghan branch, Islamic

azad University 2012.

11. Saman Fekri-Ershad. Assessing antioxidant effects of MnTBAP in the freezing media on spermatic parameters, membrane integrity and

sperm capacitation of ram spermatozoa after freezing-thawing. Fars Science and Research Branch, Islamic azad University 2012.

12. Marjan Motiei. Study and comparison of heat shock protein A2 ( HspA2) in fertile and infertile males Study and comparison of heat

shock protein A2 ( HspA2) in fertile and infertile males. Payame Noor University (Tehran).

13. Farzaneh Bassiri. Evaluation and comparison of sperm morphology ,chromatin structure in HOS positive and negative sperm from

infertile individuals. Damghan branch, Islamic azad University 2012.

35

14. Samaneh Aghajanpour. Evaluation of PLC zeta gene expression and correlation its with acrosin activity enzyme and deficiency

protamine in ICSI candidate patients. Isfahan University 2012.

15. Zohre Fathi. The role of basal bodies on evaluation of protamine deficiency with flowcytometry. Payame Noor University (Esfahan)

2012.

16. Masumeh Zareii Kheirabadi. The comparison of efficiency of DGC and DGC-Zeta for selection of sperm with low apoptosis. Shahrekord

University 2012.

17. Elahe Navaian Kalat. Evaluation of sperm acrosin activity and chromatin structure in varicocele patients before and after

varicocelectomy Payame Noor University (Esfahan) 2012.

18. Nazem Ghasemi. Comparison of sperm morphology, DNA fragmentation, protamine deficiency and HSPA2 expression before, three and

six month after varicocelectomy. Qazvin University of Medical Sciences 2011.

19. Mahsa Kheirollahi. Sperm selection based on its binding ability to hyaluronic acid for intra cytoplasmic sperm injection. Isfahan

University 2009.

12) Research Profile (preview):

A) Semen Analysis

B) Sperm functional testes:

B-1) Nuclear chromatin maturity tests:

- Chromomycin A3 staining (CMA3)

http://www.biomedexperts.com/Concept.bme/513760/Semen_Analysis

36

- Aniline blue staining (Ab)

- Aniline blue-Eosin staining (Ab-E)

- Acridine orange staining (AO)

- Sperm chromatin Dispersion (SCD)

- Sperm chromatin Decondensation

- Single cell gel electrophoresis (Comet assay)

- DNA terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL assay)

B-2) Sperm functional tests:

- Hypo-Osmotic Swelling Test (HOST)

- Gelatinolysis Test as indicator of acrosin activity

- Evaluation of acrosin activity by spectrophotometery

- CTC test for evaluation of capacitation

- Evaluation of sperm ubiquitination, methylation DNA and DNAMTs

- Evaluation of sperm apoptotic marker (Annexin and caspase)

37

- Evaluation of sperm ROS by DCF staining

- PHD test for evaluation of acrosome reaction

C) Sperm selection procedures

C-1) Routine sperm selection procedures

- Swim-up

- Density gradient centrifugation (DGC)

C-2) Novel sperm selection procedures

- Hyaluronic acid (HA) binding method based on HA receptor

- Zeta method based on sperm surface charges

- Combined density gradient and Zeta method

- MACS method based on apoptotic marker

-HOS method based on sperm membrane integrity

38

D) Human sperm freezing

E) Techniques

- Real time PCR

- Western blot

- Immunofluorescence

13) Patent

39

Documents

Curriculum Vitae - Home - Royan Institute tavallaei 95.pdf · 31. Zarei-Kheirabadi M, Shayegan Nia E, Tavalaee M, Deemeh MR, Arabi M, Forouzanfar M, Javadi GR, Nasr-Esfahani MH. Evaluation