Raúl Alvarado Arroyo Bsc. Degree in Biochemistry

EDUCATION

2012 Master Degree of Manufacturing of Advanced Therapy Medicinal Products: Cell-based

technologies: Cell & Gene therapies and tissue engineering.

Specialization as Qualified Person (Technical Director). Promoted by the Andalusian

Initiative for Advanced Therapies – IATA (1 year - 1630 hours). Universidad de Granada.

2008 Master Research Degree as part of the Molecular Biology Program.

Department of Molecular Biology. Universidad Autónoma de Madrid.

1999-2004 B.S. in Biochemistry

Universidad Autónoma de Madrid

PROFESSIONAL EXPERIENCE

2013 Production Manager

Progress and Health Foundation. Hospital Carlos Haya (Málaga)

Cell Production Unit, dedicated to the large-scale manufacturing of autologous

mesenchymal stem Cells (MSCs) from adipose tissue of patients under GMP Standards.

Responsibilities:

- Ensure that products were produced and stored according to the relevant

documentation in order to obtain the required quality.

- Approve the instructions related to production operations and ensure their strict

compliance.

- Draw up and approve production protocols.

- Verify the maintenance of the department, premises and equipment.

- Ensure that appropriate validations were performed.

- Ensure that the required initial and continuing training for personnel is done and that

such training is adapted to the unit needs.

- Ensure material´s stock and its traceability.

2006-2011 Centro de Biología Molecular Severo Ochoa (CBMSO)

Scientific Researcher in the Professor Federico Mayor Jr.´s (PhD) Lab.

Development of the project : "Regulation of the kinase of G protein-coupled receptor 2

(GRK2) by transcription factors of the Snail family: possible role of GRK2 in the process

of tumor progression” (Spanish National Plan I+D+I 2006-2008; SAF2005-03-053).

Telephone: +34 665341773 E-mail: raulalvaradoarroyo@yahoo.es

Extended Profile: http://www.linkedin.com/pub/ra%C3%BAl-alvarado-arroyo/43/b8b/838

Project gained experimental experience:

● Molecular Biology Techniques: PCR genotyping, Quantitative RT-PCR, Site-directed

mutagenesis, DNA and RNA isolation and purification.

● Protein techniques: Western-Blot, Kinase assays, immunoprecipitation, column

chromatography purification, antibody and protein conjugation and optimization, ChiP.

● Cell culture techniques including mammalian cells, DNA cell transfection, primary

mouse keratinocytes culture. Detection techniques of DNA synthesis.

● Animal Research: Conducting experiments of chemical carcinogenesis in skin mice

models. Harvesting of samples for histology, DNA sequencing, Microarrays of gene

expression, etc.)

● siRNA technology, analysis of gene expression.

● Immunohistochemistry, Tissue Arrays, several Tissue Staining Techniques.

2006 Merck Sharp & Dohme Spain

Data reviewer. Medical Department

Functions: Review and validation of in silico data from clinical trials. Dastabase querying,

interpretation of the outcome of the consultation and resolution of errors. Reporting to

the monitors of these studies.

2005 Centro Nacional de Investigaciones Oncológicas (CNIO)

Animal housing unit technician. Pathogen-free facility (SPF).

Features: Technical supervision and control of genetically modified mouse lines (GEM).

Acquired experience: 1. Crossing and diagnosis of pregnancy. 2. Identification of mice by

marking. 3. Weaning and sexing. 4. Clinical and behavioral Inspection, health monitoring

and sampling for analysis. 5. Necropsy

2004-2005 Serono Spain (Merck-Serono actually)

Features: Laboratory Technician in the R&D-OTS (Operations Technical Support)

department, dedicated to process control and optimization of recombinant drug

production (growth hormone and follicle stimulating hormone).

Experience: Management and monitoring of industrial-scale cell lines. Knowledge of

operating under GMP conditions (quality standards of the Pharmaceutical Industry).

OTHER SKILLS

Certificates

Certification for handling laboratory animals (B category; Experimental). June 2014. Animalaria S.L.

Languages

English: FIRST Certificate in English by Cambridge University (72/100). Achieved B2 level according to

the Common European Framework (CEF). December 2014.Courses by International House, in England

and Ireland.

Information Technology

● Proficiency with computer office tools (Word, Excel, Outlook, PowerPoint) and scientific databases:

(Medline, Oncomine, Gene Ontology Database, KEGG, Transfac, etc.)

●Management of images processing software: Zeiss LSM, ImageJ.

● Processing of data derived from gene expression Microarrays.