CONFIDENTIAL. The United States Ami-Doping Agency Hilf B

UNIVERSITY OF CALIFORNIA, LOS ANGELES UCLA

UCJ-A OLYMPIC ANALYTICAL LABORATORYOSPAftTMSHT OF PHA3U4ACOLOCY

i;CLA SCHOOL OF MECTCIKE.

LOS ANGELES, CALIFORNIA 93025.IPKOHEOLOSZJ-JSJJ

FAX !3 105 SO*- 9077

CONFIDENTIAL.

May 27, 2004

Terry MaddenThe United States Ami-Doping Agency2550 Tenderfoot Hilf St., Suite 200Colorado Springs, CO 80906-7346

RE: Specimen number 1

Dear Mr. Madden

Please find enclosed the documentation package for the screen, the A confirmation,and the B confirmation on the case identified above.

Enclosed are computer generated images and graphs supporting our conclusion andthe drug testing report.

Please feel free to ca?i if you have any questions

Sincereiy

CONFIDENTIALDOCUMENTATION

SAMPLE IDENTIFICATION:Organization requesting test: USADADate of sample collection: Mar 16, 2004

ID: OOC

Substance identified:"recomfcinant human Erythropoiefin (rHuEPO)

TABLE OF CONTENTS

CHAIN OF CUSTODY DOCUMENTATION FOR SAMPLE CONTAINER Page

Official record, lab copy . . . , . , . . . , 4

Courier documentation 5

Box chain of custody"docurnsntation , . . . . , , , , . , . . . . . , . , . 6

Laboratory confirmation of receipt of sample , . , . , , 8

Individual seals chain of custody documentation 9

"A" Screen aliquot chain of custody documentation 10

"A1' bottle chain of custody documentation 12

"A" confirmation aliquot chain ol custody documentation . . . . . . . . . . . 13

"A" sample stability test aliquot chain of custody documentation 15

"B" bottle chain of custody documentation . 16L'B" confirmation aliquot chain of custody documentation 17

"B" sample stability test, aliquot chain of custody documentation . . . . . . 18

Description of 'the EPQ assay . , , , . . 19

"A" SAMPLE SCREENING DOCUMENTATION

Figure 1; Electropherogram with isoform patterns - Includes this sample,Standards, Negative QC, Positive QC, and one other sample inbatch , 24

Results of Screen . , . . , . . , . , . . . . . 25

Page 2 of 33

"A" SAMPLE CONFIRMATION DOCUMENTATION

Figure 2 ; Eiectropherograni wUh :sotonf> patterns - Includes thissample, Standards, Negative QC, Positive QC ' . . , . . , 26

Figure 3 : Isoform profile A confirmation 27

Results of A confirmation , 25

"B" SAMPLE CONFIRMATION DOCUMENTATION

Figure 4 ; Efectropherogram with isofcrm patterns - Ircludes thissample, Standards, Negative QC, Positive QC 29

Figure 5 : isoform profile of 6 confirmation 30

Results of B confirmation , , , 31

SAMPLE STABILITY DOCUMENTATION

Figure 6 ; Eiectrophsrogram of stability test (A confirmation) . . , . , , . , 32

Figure 7 : Eiectropherogram of stability test (B coni=ra-afon) . . , . - . . . . 32

Figure 8 : Overlay of isoform profiles of screen, A-, ana 3-confinnatfon 33

Page 3 of 33

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CHAIN OF CUSTODY DOCUMENTATION

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Page 18 of 33

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DESCRIPTION OF METHODS

Prelection of Recombtnant ErythfopojetJC- Proteins

SCREENING PROCEDURE

Unne samcies that are tested fo-r en/thropoietic p'cteins are screened fi.'st. If thescreening results suggests that a prohibited substance is present the samples aresubjected to an A-confirrnation procedure. in the UCLA laboratory the screen and theA-carnirmation are performed by the same method. The main difference between thescreen and the A-confirmation is that in She former several samples areelectrophcresed on one gel next to each other, whereas h the tetter the samples are

separated by extra space.

The procedure described below detects recombinani human erythropoietin (rHuEPO)and darbepoef;r. The analysis consists of four steps: sarnpie preparation; isoeiectricfocusing (!EF), immuno-blotling, and visualization. The following description isintended to be a summary for the non-specialist.

ple preparation

Preparing samples for IEF analysis involves two steps; 1} enzyme deactivation, 2)filtration, and concentration. The first step is to deactivate enzymes thai could destroythe rHuEPO thereby foiling the analysis, ft is .done by adding protease inhibitors(chemicals/ to the urine. The second step is to remove materials from the urine thatare irrelevant and to concentrate the proteins. This is accomplished with specializedfilters that retain molecules with high molecular weight, such as rHuEPO. The urine is

placed in a cup that has the specialized filter. The cup is placed in a centrifuge whichspins at high speed. This forces the low molecular weight material to pass throughthe Hlter. The rHuEPO and other proteins with similar molecular weights are retained(the retentate) on the filter. This step is repeated one more time. If rHuEPO is

present in the urine, it will be in the final retentate. The final retentate is a liquid. A

Page 19 of 33


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small portion cf it is used to estimate the concentration of rh'uEPO by animmunoassay. Another portion is soottsd on a gei (described beiow). Theimmunoassay provides an estimate of the amount of rHuEPO in the urine sample,Tr-is number is useo to adjust the concentration of HuEPQ in the retentate to anoptimal value. No adjustment is needed if the estimated concentration is low. if theestimate is high the retentate is diluted. Twenty rn;cro!uers of the retentate are appiieato the gel.

isoeJecitic focusing

Glycoproteins such as darbepoetm and rHuEPO are molecules that carry positive andnegative charges. The net charge o< the glycoprotein is the algebraic sum of all thepositive and negative charges. Each molecule has one net charge which is positive,negative, or neutral depending on the pH. The isoefectric point (pi) is a fundamentalcharacteristic of proteins. It is the pH value at which the molecule is electricallyneutral because the number ol positive charges on the molecules are exactlybalanced by the number of negative charges.

The gel is a jelly-like material that serves as the 'platform' for the electrophoresis. Thegel is about 25 by 12 cm (length and width) and about 1 mm thick. The ge! isprepared by mixing various chemicals in a flask and pouring the mixture into a cast.Just before the pour, a reagent is added thai causes the materials to 'gel.' After thegel 'hardens' to the consistency of a flexible jelly-like material, the cast is removed andtrie gel is placed flat on the surface of the etectropnoresis instrument.

Before the samples are put on the gel, a pH gradient must be set up. Electrodes areattached ;o the gel and connected to the electrophoresis unit. One of the electrodesattached to the gel is the anode and the other is the cathode. When theelectrophoresis unit is turned on it sets up an electric circuit between the cathode

(negative pole), and the anode (positive pole). When the current is applied themolecules used to set up the pH gradient migrate. The charged molecules, migrate inthe direction of the electrode bearing the opposite charge. Thus negatively charged or

Page 20 of 33

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more acidic molecules migrate towara the anode - the electrode with the positivecharge and vice versa. A current is applied to the gel for half an hour to establish thepH gradient.

The current is turned off. The samples and standards that are to be electrophoresedare 'spotted' onto the gel by adding-a small volume (20 micro-liters) of each sample toa o>ece of filter paper <10 by 5 mm) thai has been placed on the gel. The pieces areplaced 1 cm apart close to one sdge of the gei. The gel can accommodate about 24such pieces. In this way the surface of the gel is divided Into 24 imaginary lanes.

Each sample of standard is sported in one lane. The number of samples andstandards that are processed on one gei is determined by the intent of the anaiysis.Typically we use one or two different standards, one or more control samples (contentknown}, and several unknowns (content not known). Typically the standard is purerHuEPQ. or pure darbepoetrrh or a mixture of the two. A typical control urine is aurine obtained irorr- a subject to whom we have administered rHuEPO,

The current is turned back on which causes the glycoproteins that have been spottedon the gel to begin to move or migrate. The .total number of hours is selected suchthat all the moiecuies have sufficient time to migrate or move to their isoelectric point.Once they reach the isoelectric point they remain stationary.

With thrs background, one can now interpret an electfopherogram that shows rHuEPO(see figure 1). rHuEPO has 5 bands which are referred to as isoforms. An isoform isa subset of the rHuEPO molecules that has a defined pi. Not all the molecules of

rHuEPO have exactly the same chemical structure and therefore the same pi. All themolecuies ir> one band will have the same pi, that is they wifl become neutral at oneand the same pH. The molecules of darbepoetin focus near the anode side of the geibecause it has a iow pi. In contrast, rHuEPO focuses cioser to the cathode because ithas a higher pi. This explains why rHuEPO and darbepoetin are separated OR the gelby several centimeters of physical space.

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At this slags the bands are separated on the gel The task now is to transfer them oilthe gel. This is accomplished by 'blotting'. Blotting is a procedure for transferringp'Ole»ns from one surface to another. Two blotting procedures are used fblot 1 andblot 2). The firsLbjat transfers the rHuEPO from the gel to membrane #1. Membrane#1 is incubated with antibodies against EPO proteins. The second blot transfers theantibodies from membrane #1 to membrane #2,

The gel is removed from the electrophcresis ptate, washed with a Duffer, and placedbetween two stacks of paper that have been soaked in a special blotting buffer. Thisstack (or sandwich) is placed into an instrument which is designed to apply anelectrical charge across two plates. The stack is placed between the two plates anathe current is turned on for 30 minutes. The instrument fs referred to as the 'Blottingunit.' During b!o! #1 the rHuEPO 'travels' from the gel to membrane #1. Membrane #1is a mirror image of the material that was on the gel.

Next membrane #t is bathed in a solution of antibodies against EPO. Theseantibodies are very special because they specifically bind to endogenous human EPO,rHuEPO, and closely related proteins such as darbepoetin. The specificEPO/darbepoetin antibodies are obtained from mice that have been immunized andtherefore make antibodies that react with darbepoetin and rHuEPO. Because we alsouse another antibody later in the assay, we sometimes refer to this antibody the 'first'or primary antibody.

In the next stage, referred to as ihe second blot, the primary antibody is transferredfrom the first membrane to a second membrane (membrane #2). The rnofecufes ofEPO and darbepoetin remain on the first membrane, but the antibodies against them(primary antibody) are transferred to the second membrane. The test detects theprimary antibodies that mark the location of the darbepoetin' and rHuEPO, Thesecond blot is accomplished like the first blot. Then ihe second membrane is

Page 22 of 33

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incubated la a solution containing a second ..ant.it/-3dy 'hat binds specifically to th

primary anybody.

The locatio- of the second antibody on membrane %2 matches the location of EPO oroarbepoetin on membrane #1. We need a way to visualize the second antibody. To

this end, a marker protein which binds to the second antibody is used. Next a special

substance is adoed that emits light when it comes in contact with the ma'ker protein.The emitted light is then captured with a special digital camera. The final image

(eiectropherogram) is used !o evaluate the results.

CONFIRMATION PROCEDURE

The confirmation procedure is very similar to the screening procedure. The sample

preparation, isoelectric focusing (iEF). immuno-bJotiing, and visualization are identicalto the screening procedure. Trie only difference is the types and number of samplesspotted on the confirmation gel For the A-confirmation four fanes contain a standard(darbepoetin and rHuEPO), and there is one lane each for the Positive Quality

Control, the Negative Quality Contro!., and the sample that is being confirmed.

Page 23 of 33

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sample

Electropherogram corresponding to screening a batch of urine samples forerythropoietie proteins. Each sample is contained in one lane. There are six ianes.The bands or isoforms corresponding to -rHuEPO, darbepoetin (NESP), or endogenousEPO have the appearance of rungs of a ladder. There are two lanes (lanes 1 and 6,numbering from left to right) containing a standard consisting of a mixture of rHuEPOand darbepoetin, lane 2 contains a negative quality control urine sample., and lane 3 apositive quality control urine sample. Lane 4 is a sample from another athlete andlane 5 is sample

Page 24 of 33

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SAMPLE A SCREEN CONCLUSION

The two-band ratio is greater than 1 .IS and the percent basic isoforrns is greater than

80%. therefore an A-comirrrtation is carried out. (For the origin of the number 1.19see pubi'catio": Breidbach A, Catlln DM, Green GAr Tregub I.. Truong H. Gorzek J.Dejection of rHuEPO in Urine by isoeieciric focusing. Clin Chem 2003; 49'901-907)

Page 25 of 33

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the appearance of rungs of a ladder. There are four fanes containing a standard

consisting of a mixture o' rHuEPO and darbepoefin. These are lanes 1, 4, 5, and 7

with the numbering starting from the left-most lane.. The negative QC sample is in

fan© 2 and the positive QC sample is in lane 3. The lane containing urine 4BH& is

lane Q,

Pace 26 o? 33

UCLA Olympic Analytical' LaboratoryUCLA Schooi of Medicine

2122 GranvUle Ave Los Angeles CA 90025 1SQ/JEC 17025IOC Accredited Phone (310) 825-2635 FAX (310) 206-9077 Biological Testing


!??&£!

Figure 3: Graphicrept reEsnia t ion of 'Cos cancipa t t e rn - A cor:fiirfria;; ';cn

Densitomet-ry has been used to express the darkness (density) of the bands in Fsgure2 as numerical values. Plotting these values as a function of the band number resultsin graphs showing the relative peak height of the bands on the Y-axis and bandnumber on the X-axis, The band labeled 0, which is marked by a vertical line, isdefined as the band with the same isoelectric point (p!) as the most acidic band in therHuEPO standard. Starling from band 0, the successive bands in :;he direction of theanode are considered acidic' and are labeled 1, 2, 3, 4,... Similarly the bands In thedirection of the cathode are considered 'basic' and are labeled -1, -2, -3r and -4,

The shape of the graph for the negative QC is characteristic of a. negative sample.The peak apex occurs at band 2 and the area under the curve is predominantly to theright of the vertical line. The shape of the graph for the positive QC is charactefistic ofa positive sample. The peak apex occurs at band -1 and the area under the curve ispredominantly to the ieft of the vertical line.For sample %$38i^$Sfe peak apex is on the left of the vertical fine like that of thepositive QC and the area under the curve is predominantly on the left of the verticalline.

Paae 27 of 33

fVl" UCLA Olympic Analytical LaboratoryI.-rlA UCLA School of Medicine*w!U^ 212.2 Gran v! He Ave Los Angeles CA 90025 ISG/JEC 17025

IOC Accredited Phone (3101 825-2635 FAX' (310) 200-9077 Bfologtca! Tastings: 1420-Ot

SAMPLE A CONFIRMATION CONCLUSIONS

The A confirmation aate indicate the presence of rHuEPO according to criteriapublished in a peer-reviewed journal by the UCLA. Olympic Laboratory <Breidbac-h A,Catlin DH, Green GA, Tregub I, Truong H, Garzek J. Detection of rHuEPO in Urineby isoeieetr'c focusing. Clin Chem 2003; 49:901-907.) In summary these criteria are;

1) the isoform bands that focus in the same area as the rHuEPO standard aredarker than other isoform banos of the sample.

2} these bands have the same pi as the corresponding bands of the rHuEPQstandard;

3) the sample contains the three bands that correspond to the p! of bands 0, -1, and -2 of the rHuEPO standard (see description for figure 3};

4} the Two-Band Ratio' is greater than 7. The upper 99% confidence intervalfor the two-band ratio is 1.19. (Note: the ratio is not a linear function (seeBreicJbach eta!,Cim Chem 2003; 49:901-907}

2j JPerceiii basig jgof

in addition, the sample is positive lor rHuEPO according to the 'percent basicfsoforms' criteria. The percent basic isoforms is 90%., This method has been the

subject of discussion in various expert committees hosted by the IOC and it has beendescribed in an extended abstract by Pascua! et ai. (Schaenzer el.a!.(eds.) Recent

Advances in Doping Analysis/ Proceedings of the Manfred Do-nike Workshop.

2002:135-144). In addition the method is discussed in a Court of Arbitration for Sportdecision (CAS 2001 /A/3 45 Roland Meier v/ Swiss Cycling} wherein a value greaterifa3!L§£% wss deemed to be positive

Page 28 of 33

tOC Accredited

UCLA Olympic Analytical LaboratoryUCi-A School of Medicine

2122 Granvilie Ave Los Angeles CA 90025Phone (31C) 8.25-2635 FAX (310) 206-9077

ISO/1 EC 17025Biologica! Testing


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Each sample Is contained in one lane. There are i lanes ?n the figure. The bands orisoforms corresponding fo rHuEPO. darbepoetin (NESP), or endogenous EPO havethe appearance of rungs of a ladder. There are four lanes containing a standardconsisting oi a mixture of rHuEPO and darbepoetin. These are lanes 1, 4, 5, and 7with the numbering starting from the left-most lane. The negative QC sample is inlane 2 and the positive QC sample is in lane 3. The lane containing urine

lane 6.

Page 29 of 33

JOG Accredited

UCLA Olympic Analytical LaboratoryUCLA Schooi of fvledicirss

2122 Granvilie Ave Los Angeles GA 90025Phone (310> 825-2635 FAX (310) 206-9077

(SO/I EC 1702SBiological Testing


I „

I ••'•' -;

Densitometry has been used to express the darkness (density) of the bands in Figure

2 as numerical values. Plotting these values as a function of the band number resultsin graphs showing the relative peak height of the bands on the Y-axis and bandnumber on the X-axis. The band labeled 0, which is marked by a vertical tine, isdefined as the band with the same isoeiectric point (pi) as the most acidic band in therHuEPO standard. Starting from band 0, the successive"bands in the direction of%eanode are considered 'acidic' and are labeled 1, 2, 3, 4,... Similarly the bands in thedirection of the cathode are considered 'basic" and are labeled -11 -2, -3; and -4.

The shape of the graph for the negative QC is characteristic of a negative sample.The peak apex occurs at band 2 and the area under the curve is predominantly to theright of the vertical line. The shape of the graph for the positive QC is characteristic ofa positive sample. The peak apex occurs at band -1 and the area under the curve ispredominantly to the left of the vertical fine.For sample ££g the peak apex is on the left of the vertical line like that of thepositive QC and the area under the curve is predominantly on the left of the vertical

line.

Page 30 of 33


2122 Gfapvii'e Ave Los Angeles CA 90025 ISO/ISO "17025SOC Accredited Phone '310: S25-2635 FAX (3101 206-9077 Biological Testing

Certificate; 1

SAMPLE B CONFIRMATION CONCLUSIONS

.1) .TPe. two-bans' ratio :

The A confirmation data indicate V~i3 presence o' ;HuEPO according to criteriapublished in a peer-reviewed journa! oy the UCLA Olympic Laboratory (Breic'bach A.Catlin DH: Green GA, Tregub f, Truong H, Qorzek J. Detection of rHuEPQ in Unneoy tsoelectric focusing, Ciin Chem 2003; 49:901-907.) !n summary these criteria are;

1} the isoform bands that focus in the same area as the rHuEPO standard aredarker than other isoforrn bands oi the sample.

2) these bands have the same pi as the corresponding bands of the rHuEPO

standard;

3} the sample contains the three bands that correspond to She pt of bands 0, -i. and -2 oi the rHuEPO standard {see description for figure 3);

4) the Two-Band Ratio' is grsaier than 6. The upper 99% confidence intervallor the hvD-banti ratio is 1.19, (Note: the ratio is not a iinear function {seeBreidbach et.al.Clin Chem 2003; 49:901-907)

2} Percent basic i

In addition, sample fiSQ^ is positive for rHuEPO according to the 'percent basicisoforms' criteria. The percent basic isoforms is 89%, Thfs method has been thesubject of discussion in various expert committees hosted by the IOC and ii has beendescribed in an extended abstract by Pascuat et. al. (Schaenzer et.aUeds.) Recen'Advances in Doping Analysis/ Proceedings of the Manfred Don^ke Workshop.

2002:135-144). in addition the method is discussed in a Court of Arbitration for Sport

decision (CAS 2001;'A/345 Roland Meier v/ Swiss Cycling) wherein a value greaterthan 80% was deemed to be positive

Page 31 of 33

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To veny that the tsoforms are siabis In the sample, we adjusted the pH to 5 with 0,1nol/L HCf. We added Pepstatin (15^mo!/L to 500^1) and Complete stock solution (1% cfa 1 tablel'2mL solution;' to 0.5 mL of sample (Pepstatin and Complete are proteaseinhibitors.). Afte' 15 min ai room temperature 3QGmU rHuEPO were added and the

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urine vi/as incubated at 37CC overnight. 20«L of the treated urine sample weresubjected to isoelectric focusing ana immunoblotting. This study was carried out withsample #$MHP The rationaie Is if there is anything that causes a shift of theisoform pattern of the urine sample the well-Known pattern of the added rHuEPO

would change.Figures 6 and 7 show the elec'ropherog-arns of the above experiments. It can be seenthat the isoform pattern of the addea' rHuEPQ did not change.

Page 32 of 33

jp

IOC Accredited

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17025Biological Testing

Certificate," t420-0l

Figure 8 sh;:ws an overlay Q* 1^= iscform patterns o! the screen, ;he A- : and the B-confirmaticn ot sample @H . This funher verifies tât there is nc changg jn thelocation of the iso'orms between the three analyses.

I--?- A Sir !.««,-.*!* ![-•»•• Sinw. ( !

Figure 8: Overlay of the craphicreprescTitet ions of th« band pat tern ofs c r e e n , A - , and B-conf i r jnac ion of sample

Page 33 of 33

FADE

UNIVERSITY OF CALIFO.RKIA, LOS ANGELES

CQJJFiDiNTfAL

UCLA

BCU. OLYMPICDEfAJXTKENT

L'CIA SCHOOL Of M8&rC!KBJljj CftANYTLLS XYBS'Xre.

LOS ANoeuss. CAt1? |s Â scowPHOXEO'JC) tli-iSJi

FAX {S3S1

June 211 2004

Terr/ MaddenThe United States Antf-Doping Agency2550 Tenderfoot Hill St., Suite 200Colorado Springs, CO 80906-7346 -

RE: Specimen number

Dear Mr. Madden

Please find enclosed an addendum to tne documentation package for the caseidentified above.

Please feel free to calf ff you have any questions.

Sincerely,

Don H. Catlin, M.D,Director

CONFfDENTiAtDOCUMENTATION

SAMPLE IDENTIFICATION:Organization requesting test: USADADate of sample collection: Mar 16, 2004

OQC

Substance identified: reeomWnant human Brythropoietin

TAfiLIE OF CONTENTS

"A" SAMPLE CONFIRMATION DOCUMENTATION

Table 1 : Results of o'ensitometry


Table 2 : Results of tfensitometr/ , . . , . .

Page 2 of 4

J'Jl^-2i •• O<3 1B- -5S F R O H • - l O : PAC:;


2122 Granviile Ave Los Angelas CA 9C5025!OC Accreditad Phone (310} 825*2655 FAX (310} 206-9077

Cftrtffieata: 1420-61

Band•4

-3

-2

-10

1

2

3

4

5

Peak area

37357

232807

463738

553275

382344

39495

24331

14888

10689Table 1; Peak areas of the A confirmation of this sample

Densitometry has been used to convert the band densities (darkness) into areas(see documentation package page 27), From these areas the Two-band ratio'and the 'Percent basic isoforms' have been calculated. For the Two-band ratio*the sum of the areas of bands '-1' and '-2' is dlvfa'ed by the sum of the areas ofbands '1' and '2'.

The value for the TBR is 7.3 {(553275*463736)/(99292+39495)).

For rhe 'Percent basic isoforms' the sum of the areas of the basic bands {'0', '-1', -2', etc.) is divided by the sum of the areas of all bands and than multiplied by 100.

The value for the 'Percent basic isoforms'' is 89,8 %,

Page- 3 of 4

JUN.-2 1 - 0''1 1 K : bfc"

j"y UGIA Gfrmpte Analytical Laboratory00 UCLA "School Of I

2122 GranvlU* AY* Lo$Angele«CA i0026IOC AseredH*ef Phont 1310} 825-2635 FAX (3tO) 2G8-9S77

Band-4

-3

-2

-1

0

1

a3

4

5

Pwrtt ar«a221SO

204283

510906

565432

522979

125102

47359

29S72

21258

12197Table 2: Peak areas of the S confirmation of this sample

has been used to convert the isand densstfe$ (darkness) info areas(see documentation package page 27). From these areas the Two-band ratio'and the 'Percent basic isoforms' have fasen calculated. For the Two-band ratio'the sum of the areas of bands '-1' and '-2' is dividid by ths sum of the areas ofbands 'V and-'£'.

The value for U\a TBR Is 8.8 {(665432+51090e)/(126182+47369}).

For the 'Percent basic isoforms' the sum of the areas of ths baste bands ('0', '-V, '-21, «tc.) is divided by ths sum of the areas of alt bands and then multiplied by 100.

The value for the 'Percent basic isoforms' is 89,1 %,

Page 4 of 4

UNIVERSITY OF CALIFORNIA, LOS A X C F I J K S TCLA.

CONFIDENTIAL

UCLAOLV),*?;r ANAi.ltiCAt:IJEPARTW-EN-r Or VHA.IMACGJ.OG V

SX-..A SCHOOL Gf MBVIC'NEl:r; OS-.NNVri.-LE A V E N U E

LOS ANSli-VS. î

FAX ( 3 : 3 : ZJf- .TC-T

November "\2, 2004

Terry MaddenUnited States Anii- Doping Aaency2550 Tertdenoot Hill St., Suite 200

olorado Springs, CO 80906

RE; Specimen numtoe?f

Dear Mr. Madden.

In response to your fax of today, please find enclosed an addendum to thedocumentation package for the case identified above.

In ouf publication (Breictoach A, Cailin OH, Green GA, Tregub i, Truong H, Gorzek J.De!ect?on of rHuEPO in Urine by isoeieclric focusing. Cisn Chem 2003: 49:901-307} westate thai a TBR value greater than 1,19 is associated with a risk of 1 in 100. Sincethat publication we have increased our rHuEPO-free conirol population to 685sampies, and based on that data, a TBR value of greater than 1.8 is associated wilh arisk of 1 in 100,000. That is, if 100,000 samples were analyzed we would expect tofind -one sample with a TBR greater than 1.8.

Please feel free to call if you have any quesiions

Sincerely,

Don H. Cailin, M.D.Director

CONFJLDENTJALDOCUMENTATION

SAMPLE IDENTIFICATION:Qrgaruzaticn requesting test: USADADate of sample coileciion: Mar is, 2004Site ID: OOC

ubstance identified: recombinant human Erythropoietln (rHuEPO)

TABLE OF CONTENTS

CRITERION FOR POSnWJTY

"A" SAIVIPLE CONFIRMATION DOCUMENTATION

Figure 1 : £;ecfrapherogram with isoform patterns and band labelingaccording to WADA TD2GG4EPO draft of Oct 13, 2004 4

Figure 2 : Densitogram of this sample , , 5


Figure 3 : Eieciropherogram v/.t" isoform patterns and band iabelinoaccording to WADA TD2004EPO draft of Oct 13; 2004 . . . , " . , - . 6

Figure 4 : Densitogram of this sample 7

Page 2 of 7

UCLA OSympic Analytics: laboratoryUCLA School of Medicine

2122 Granvilie Ave Los Angelas CA 90025 ISO/tEC 1T02S!OC Accredited Phone (310) 825-2635 FAX (3101 206-3077 Biological Tasting


As slated in the documentation oackage for this sample, dated Apr 15. 200^: ourcrmary cnisrion for positivity is the "two-band ratio" ^TBRK The concept c* this ratiohas undergone peer review and was published in 2003 {Breidbach A, Ca-Jin DM.Green GA, TregtiD 1. Truong H, Gorêk J. Deteclbn of 'HuEPO In Urine by fsoeieclrifocusina, Ciin Chen 20C3: 43:901-9075.

Page 3 of 7

IOC Accredited


2122 Granviile Ave Los Angeies CA 90025Phone (310} 825-2635 FAX (310} 206-9077

ISO/IEC 17025Biotogica) Testing

Certificate: 1420-Ot

ay>LLIzo£LLU3

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Figure 1: E1 e c t r G i:- h e r o g r a n c o r .v e s c> o r. d 1 n cto the ft conf i rma t i cn of fcJi.is sancle.

Densitometry has been used to convert the band intensities (darkness) into numericalvalues (see documentation package page 27). Those numerical values (peak heights)are displayed next to the individual bands in parentheses. Aiso shown are the bane'IDs (1, 2, 3, 4,...) according to the World Anti-Doping Aaency's (WADA) technicaldocument TD2004EPO drafi of Oct 13, 2004,

It can be seen thai band 2 is the most intense band in the entire iane, that the secondmost intense band is band 3, and that there are three consecutive, acceptable bands(bands 1, 2, and 3} in the "basic1' area. Therefore the identification criteria of WADATD2004EPO draft of Oct 13, 2004 are satisfied,

Page 4 of 7

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UCLA QJyr^pic Analytical laboratoryUCLA School of Medicine

2(22 Grar.viile Ave Los Angeles CA 90025Phone |310} 825-2635 FAX (310) 206-90??

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Certificate; 1420-Ot

Figure 7. :Densit~jran of ]ane US ADA

Pane 5 of /

IOC Accredited

UCLA Olympic Analytical Laboratory-UCLA School of Medicine

2122 GranvIHe Ave Los Angeles CA 90025Pnone 1310} 825-2635 FAX (310) 206-907?

SSOrtEC 17025Biological Testing


a.£0LU

OaLU3

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3Iropherogi"3~; correspond in;raarion of this sanr.ple

Densitometrv has been used to convert the band intensities (darkness) into numericalvalues (see documentation package page 27), Those numerical values (peak heights)are displayed next to the individual bands in parentheses, Also shown are the bandIDs [1, 2, 3, 4....) according to the World Anti-Doping Agency's (WADA) technicaldocument TD2004EPO draft of Oct 13, 2004,

it can be seen thai band 2 is the most Intense band in the entire lane, thai the secondmost intense band is band 1, and that there are three consecutive, acceptable bands(bands 1, 2, and 35 in the "basic" area. Therefore the identification criteria of WADATD2004EPO draft of Oct 13, 2004 are satisfied.

Page 6 of 7

fOC Accredited

LICLA Olympic Analytics' Laboratory .UCLA School of Medicine

2122 Oranville Ave Los Angsies CA 9Q025Phone (310} 825-2635 FAX (310) £06-9077

iSO/fEC 17025Biologica! Testing


Fiqure ograni cf j ane US&f.

Page 7 of 7

Documents

CONFIDENTIAL. The United States Ami-Doping Agency Hilf B