Commercial Diver Repairing Damaged Structure

AQUATIC BIOTECHNOLOGYINTRODUCTION TO BIOTECHNOLOGY

ENDRIKA WIDYASTUTI, S.Pt, M.Sc, MP

MOLECULAR GENETIC OF AQUATIC MICROORGANISM

OUTLINE

MOLECULAR GENETIC OF AQUATIC MICROORGANISM

OUTLINE

INTRODUCTION TO

AQUATIC BIOTECHNOLOGY

THE USE OF LIVING MARINE (AQUATIC) RESOURCHES AT (ECO)SYSTEM, CONCEPT, ORGANISM OR MOLECULAR LEVEL TO PROVIDE BENEFICIAL SOLUTION

INTRODUCTION TO

AQUATIC BIOTECHNOLOGY

• Increasing the world’s food supply

• Improving seafood safety and quality

• Identifying novel compounds for the benefit of human health and medical treatments

• Seeking new approaches to monitor and treat disease

• Bioprocessing• Restoring and protecting

marine ecosystems

MOLECULAR GENETIC OF AQUATIC MICROORGANISM

OUTLINE

AQUATICULTURE

DEFINITION: RERARING OF AQUATIC ORGANISMS UNDER CONTROLLED OR SEMI CONTROLLED CONDITIONS

AQUATICULTURE

What Can We Grow?

• Food—finfish, crustaceans,

mollusks, frogs, algae

• Baitfish—fathead minnow,

golden shiner

• Sportfish—bass, bluegill,

walleye

• Ornamentals—tilapia, goby,

• Feeder fish —goldfish,

shiner, minnow

• Biological supply houses

AQUATICULTUREPhases of Aquaculture

Broodstock

Management

Hatchery

Production

Nursery

Production

Grow-out

Harvest &

Market

MOLECULAR GENETIC OF AQUATIC MICROORGANISM

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MOLECULAR GENETIC OF AQUATIC ORGANISM

MOLECULAR GENETIC OF AQUATIC ORGANISM

MOLECULAR GENETIC OF AQUATIC ORGANISM

Class of polypeptides produced by certain vertebrates,

plants, fungi and bacteria that permit their survival in subzero

environments. AFPs bind to small ice crystals to inhibit growth

and recrystallization of ice that would otherwise be fatal.

Antifreeze proteins (AFPs)

pleuronectes americanus Nothen cod

MOLECULAR GENETIC OF AQUATIC ORGANISM

“GREEN GENES”

• Green Fluorescent Protein (GFP)• Produced bright green glow when

exposed to ultraviolet light• A way for fish and other organism

to find each other in dark environment

• Used for making unique REPORTER GENE (to detect expresion gene of interest)

aequorea victoria

MOLECULAR GENETIC OF AQUATIC MICROORGANISM

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MEDICAL AND NON MEDICAL APPLICATION

CALCITONIN

Thyroid hormone that

stimulates calcium uptake

and bone calcification and

inhibits bone digesting cell

Osteoporosis

Ex: Salmon

MEDICAL AND NON MEDICAL APPLICATION

SQUALAMINE

A STEROID

POTENT ANTIFUNGAL

AGENT THAT CAN USED

FOR TREAT LIFE AIDS AND

CANCER PATIENTS

EX: SQUALUS ACANTHIAS

MEDICAL AND NON MEDICAL APPLICATION

ANTI- ANGIOGENESIS

FORMATION OF BLOOD

VESSEL

ANGIOGENESIS- A

PROCESS THAT REQUIRED

FOR GROWTH AND

DEVELOPMENT MANY

TUMORS

EX: SQUALUS ACANTHIAS

MEDICAL AND NON MEDICAL APPLICATION

LIMULUS AMOEBOCYTE LYSATE (LAL) TEST

Extract of blood cell from

horse shoe

Detect bacterial endotoxin

EX: Horse shoe

(L.polyphemus)

MEDICAL AND NON MEDICAL APPLICATION

MEDICAL AND NON MEDICAL APPLICATION

MEDICAL AND NON MEDICAL APPLICATION

MEDICAL AND NON MEDICAL APPLICATION

POTPOURRI PRODUCT

carrageenan

Toohpaste

Cosmetic

Food and snack

Thermus Aquaticus

Thermotolerant bacteria

Heat stable enzyme

Salt resistant

MEDICAL AND NON MEDICAL APPLICATION

BIOMASS AND BIOPROCESSING

MOLECULAR GENETIC OF AQUATIC MICROORGANISM

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BIOTECHNOLOGY

BIOTECHNOLOGY

BIOTECHNOLOGY

BIOTECHNOLOGY

BIOTECHNOLOGY

BIOTECHNOLOGY

BIOTECHNOLOGY

vibrio fischeri

Symbiotic bacteria

Bioluminenescent bacteria

Light emitting enzyme luciferase

Detect pollutant such as organic chemicals and nitrogen containing compounds in marine environment