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Citometrie in flux
FCM Flow Cytometry
2/24/2012
Citometrie in flux - utilizari Dimensionarea particulelor (celule, componente
celulare) Numararea celulelor Determinarea viabilitatii celulelor Determinarea continutului total de ARN si ADN Determinarea continutului de clorofila Determinarea unor activitati enzimatice Evidentierea modificarilor proteinelor Evidentierea modificarilor genetice
2/24/2012
Structura unui citometru pentru citometrie in flux Sistem pentru circulatia fluidului Sistem optic
- Sursa de lumina (Laser, Lampi Hg sau Xe) - Filtre Sistem de detectie (ADC) Sistem electronic Calculator
2/24/2012
Schema unui citometru
2/24/2012
Focalizare hidrodinamicaThe image cannot be displayed. Your computer may not have enough memory to open the image, or the image may have been corrupted. Restart your computer, and then open the file again. If the red x still appears, you may have to delete the image and then insert it again.
2/24/2012
Interogarea particulelor
Signal
2/24/2012
Tipuri de filtre optice
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Sortare electrostatica in flux
2/24/2012
Spectrul luminii
2/24/2012
Principiul fluorescentei. Shift Stokes
2/24/2012
Absorbanta si emisia luminii de catre fluoroisotiocianat (FITC)
2/24/2012
Coloranti singulari
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Coloranti pereche
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Compensarea fluorescentei
2/24/2012