Chemical Approaches to Targeting Drug Resistance in Cancer Stem Cells

COST Action CM1106 3rd Working Group Meeting

Photo by Constantinos Arvanitakis, Copyright Acropolis Museum

26-27 March 2015- Athens, Greece St . George Lycabettus Boutique Hotel

PROGRAMME & LIST of POSTERS

Organizing Committee Scientific Committee Pitsinos Emmanuel, NCSR “Demokritos”, Athens [GR] Passarella Daniele, Univ. degli Studi di Milano [IT] Athanassopoulos Constantinos, Univ. of Patras [GR] Marija Balic, Medical University Graz [AT] Haroutounian Serko, Agricultural Univ. of Athens [GR] James Lorens, University of Bergen [NO] Passarella Daniele, Univ. degli Studi di Milano [IT] Botta Maurizio, University of Siena [IT] Kikelj Danijel, University of Ljubljana [SI] Athanassopoulos Constantinos, Univ. of Patras [GR]

Contact Haroutounian Serko, Agr. Univ. of Athens [GR]

stemchem@gmail.com Pitsinos E., NCSR “Demokritos”, Athens [GR] www.stemchem.org Vizirianakis Ioannis, Univ. of Thessaloniki [GR]

Secretariat Ioana Stupariu, Secretary of COST Action Michael Christodoulou, Univ. degli Studi di Milano and students of the local organizer’s teams

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PROGRAMME

26 March, 2015

09:00 Registration

10:00 Introduction: Emmanuel Pitsinos (NCSR “DEMOKRITOS” Institute of Nanoscience and Nanotechnology) Daniele Passarella (Università degli studi di Milano, Italy) Section I Chair: Marija Balic (Medical University Graz, Austria) Co-Chair: Gabriela Almeida (IPATIMUP, Porto, Portugal)

10:15 L1 Deniz Tasdemir (University of Kiel, Germany) Marine natural products against cancer stem cells

10:35 L2 Paul Murphy (National University of Ireland, Ireland) Decorated macrocycles via ring closing double reductive amination. Identifi cation of an apoptosis inducer of leukemic cells by antagonism of a serotonin receptor

11:00 Coffee break

Section II Chair: Danijel Kikelj (University of Ljubljana, Slovenia) Co-Chair: Serko Haroutounian (Agricultural University of Athens, Greece)

11:45 L3 Christel Herold-Mende (University of Heidelberg, Germany) Cancer stem cells: still the right name?

12:05 L4 Sabrina Dallavalle (Università degli studi di Milano, Italy) Novel HDAC inhibitors with a phenylcinnamic scaffold: synthesis and evaluation of antitumor activity

12:25 L5 Deniz Cansen Yildirim (Bilkent University, Turkey) Bioactivities of small molecule kinase inhibitors in liver cancer stem cells

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of Athens

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of AthensP-8

12:45 Lunch and Poster Session I (Odd number posters)

Section III Chair: James Lorens (University of Bergen, Norway) Co-Chair: Ioannis Vizirianakis (AUTH, Thessaloniki, Greece)

15:00 L6 Ana Martins (University of Szeged, Hungary) Effect of 20-hydroxyecdysone and its diacetonide derivative on the efflux pump ABCB1 upon treatment with etoposide

15:15 L7 Bartłomiej Taciak (Warsaw University of Life Sciences, Poland) Macrophages ability to non-canonical Wnt pathway activation in mammary cancer cells

15:30 L8 Gulce Iz (Ege University, Turkey) Evaluation of cancer stem cells for an in vitro vascularised osteosarcoma model?

15:45 L9 José Padrón (University of La Laguna, Spain) Synthesis and identification of unprecedented selective inhibitors of CK1ε

16:05 L10 Fumagalli, Gaia (Università degli studi di Milano, Italy) Single Nanoparticles for Cancer Therapy: Combination of Cyclopamine and Paclitaxel

16:20 L11 Nadine, Martinet (Institute of Chemistry Nice) CM 1106 Compound library and screening force

16:30 Coffee break and Poster Session I

17:00 – 18:00 MC meeting: Only for the members of the Management Committee

27 March, 2015 Section IV Chair: Daniele Passarella (Università degli studi di Milano, Italy) Co-Chair: Constantinos Athanassopoulos (University of Patras, Greece)

09:30 L12 Athanassios Giannis - Invited expert (Institute of Organic Chemistry, Germany) Modulators of the Hedgehog Signaling Pathway: Chemical and Biological Aspects

10:00 L13 Peng, Ling (University Aix-Marseille, France) Functional delivery of RNAi molecules in cancer stem cells

10:20 L14 Dinić, Jelena (Institute for Biological Research “Siniša Stanković”, Serbia) Novel tubulin destabilizing agent CKT0353 overcomes multidrug resistance in colon cancer cells

10:40 Coffee break

Section V Chair: Andreanni Odysseos (EPOS-lasis, R&D, Cyprus) Co-Chair: Alexandros Zografos (AUTH, Thessaloniki, Greece)

11:10 L15 Pierre Schembri-Wismayer (University of Malta, Malta) Differentiation screen for osteosarcoma by leukaemia maturation inducers

11:30 L16 Mattia Mori (Center for Life Nano Science, Italy) Structure-based discovery of a natural compound targeting the Gli1 transcription factor of the Hedgehog pathway

11:50 L17 Christian Muller (University of Strasbourg, France) 3D Cell Culture Models in Anticancer Drug Discovery

12:10 L18 Berta Sanchez-Laorden (Instituto de Neurociencias CSIC-UMH, Spain) MEArrays to identify microenvironments that promote mesenchymal to epithelial transition in tumour cells

12:30 L19 Panagiota Sotiropoulou (Université Libre de Bruxelles, Belgium) Early stage researchers meeting on Targeting Hedgehog signaling in cancer stem cells. Barcelona January 2015

P-9 3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of Athens

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of AthensP-10

12:45 Lunch and Poster Session II (Even number posters)

Section VI Chair: Wolfgang Link (University of Algarve, Portugal) Co-Chair: Emmanuel Pitsinos (NCSR “DEMOKRITOS”, Greece)

15:00 L20 Giovanna Damia (Mario Negri Institute, Italy) Epithelial-mesenchymal transition and cancer stem cell roles in cisplatin response in ovarian cancer xenografts

15:20 L21 Ellert-Miklaszewska, Aleksandra (Nencki Institute of Experimental Biology, Poland) Pharmacological targeting of brain tumor cells and update on COST compound library testing

15:40 L22 Stefania Pizzimenti (University of Torino, Italy) Targeting Nrf-2 and YAP as a new strategy to overcome cancer chemoresistance.

16:00 Coffee break and Poster Session II

16:30 L23 Borlak Jürgen (Hannover Medical School, Germany) Non-alcoholic fatty liver disease and cancer stem cells in the development of hepatocellular carcinomas

16:50 L24 Antreas Afantidis (NovaMechanics Ltd, Cyprus) Enalos InSilico Platform: An online decision support tool for the design and virtual screening of small molecules and nanoparticles

17:05 Best poster competition

17:30 Best poster competition award

17:40 General discussion and closing remarks

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LIST of POSTERS

P1 Verena Tiran, Christopher Rossmann, Christoph Suppan, Richard J Cote, Ram Datar, Nadia Dandachi, Marija Balic . “A feasibility study to evaluate microfilter based CTC enrichment to characterize CTCs with a putative cancer stem cell phenotype”.

P2 Antoine Millet, Cyril Ronco, Lionel Massi and Rachid Benhida. “Rational design of RNA ligands. Synthesis and biological applications”

P3 Juraj Dobiaš, Miroslav Murár, Peter Šramel, Gilles Hanquet, Martial Ruat, Hélène Faure and Andrej Boháč. “A classification of known scaffolds of Smoothened inhibitors for the identification of novel Smo modulators”.

P4 Darinka Gjorgieva, Ana Mitanoska and Darko Bosnakovski. “Mesenchymal stem cells as anticancer drug delivery vehicles”.

P5 Szilvia Bősze, Zsuzsa Baranyai, Martin Krátký, Jarmila Vinšová, Antal Csámpai, Ildikó Szabó, Zsuzsa Majer, and Ferenc Hudecz. “Contribution to the COST Action CM1106 compound library toward new collaborations: molecules and their in vitro cytotoxic/ cytostatic effect on tumour cell lines with potential activity on cancer stem cells”.

P6 Andrea Calcaterra, Patrizio Ghirga, Simone Berardozzi, Mattia Mori and Bruno Botta. “Synthesis of chalcones as inhibitors of Notch signaling pathway in the treatment of T-cell acute lymphoblastic leukemia”.

P7 Casimiro Cárdenas, Ana R. Quesada, Miguel Ángel Medina. “Evaluation of the Coffee Diterpene Kahweol as a Potential Anti-Tumor Compound targeting Breast Cancer Stem Cells”.

P8 Lisa Doyle, Paul V. Murphy. “SnCl4- and TiCl4- Catalysed Anomerisation of O- Pyranosides from Pentoses. Towards Anti-metastasis Glycosides”.

P9 Daniela Prinz, Nace Zidar, Lucija Peterlin Mašič, Danijel Kikelj, Marija Balic M, Nadia Dandachi. “Anti-tumoral effect of novel marine alkaloid analogues on breast and lung cancer stem cells”.

P10 Miodrag Dragoj, Zorica Milošević, Jelena Dinić, Ana Podolski-Renić, Jasna Banković, Sonja Stojković, Nikola Tanić, Milica Pešić, Tijana Stanković. “Reversal of doxorubicin resistance in non-small cell lung carcinoma cell lines by targeting CXCR4 receptor and focal adhesion kinase”.

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of Athens

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of AthensP-12

P11 Anna Lucia Fallacara, Ana Podolski-Renić, Jelena Dinić, Cristina Tintori, Giulia Vignaroli, Silvia Schenone, Maurizio Botta, Milica Pešić. “New pyrazolo[3,4-d]pyrimidine c-Src inhibitor as multi-drug resistance modulator”.

P12 Paolo Filipponi, Carmine Ostacolo, Bruno Cerra, Serena Mostarda, Ettore Novellino, Roberto Pellicciari, and Antimo Gioiello. “Continuous Flow Synthesis of Thieno[2,3-c]iso quinolin-5(4H)-one (TIQ-A) Scaffold: A Valuable Source of PARP-1 Inhibitors ”

P13 Nenad Filipović, Romano Silvestri, Sveva Pelliccia, Tamara Todorović, Snežana Bjelogrlić, Christian D. Muller. “Pro-apoptotic activity of aroylindole Pt(II), Pd(II) and Cu(II) complexes on THP-1 cancer cells and AsPC-1 cancer stem cells”.

P14 Karen Fox, Paul V. Murphy ”Synthesis of building blocks with potential in generating metastasis inhibitors “.

P15 Lidija Milkovic, Atilla Hunydi, Marija Balic, Ana Cipak Gasparovic “Antioxidative response of breast cancer cell lines to selected ecdysteroids”.

P16 Thomas Castanheiro, Morgan Donnard, Mihaela Gulea, and Jean Suffert. “Palladium-catalyzed cascade cyclizations: An original access to sulfur hetero cycles with potential anticancer activity”.

P17 József Csábi, Tusty-Jiuan Hsieh, Feria Hasanpour , Ana Martins, Zoltán Kele, Tamás Gáti, Gábor Tóth, Melita Vidakovic, Attila Hunyadi. “Oxidized metabolites of 20-hydroxyecdysone and their activity on the Akt phosphorylation”.

P18 Khodr Issa, Ashwin Narayan, Panayiota Stylianou, Yiota A. Christou, Constantinos Pitris, Rosella Galli, Andreani D. Odysseos. “γ-Tocotrienol and γ-Tocopherol modulate self-renewal potential in tumors with differential expression of the Epidermal Growth Fac tor Receptor ”.

P19 Danijel Kikelj, Nace Zidar, Uroš Trstenjak, Janez Ilaš, Hélène Faure, Lucile Hoch, Martial Ruat, Andrej Bohac. “Benzoxazine-2-carboxamide derivatives as potential small-molecule modulators of Hedgehog signaling ”.

P20 Teresa Martins, Ravi Kiran Reddy Kalathur, Filipe Carvalheiro, Richard Hill, Matthias Futschik, Wolfgang Link. “Using analysis of The Cancer Genome Atlas (TCGA) Database to identify candidates for diagnostic and prognostic biomarkers in melanoma”.

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P21 Zorica Milošević, Jasna Banković, Miodrag Dragoj, Jelena Dinić, Tijana Stanković, Sonja Stojkovic, Verica Paunović, Aleksandra Isaković, Milica Pešić . ”Establishment of chemore sistant anaplastic thyroid carcinoma with P-glycoprotein and BCRP expression“.

P22 Loana Musso, Raffaella Cincinelli, Sabrina Dallavalle, Franco Zunino, Anna Lucia Fallacara, Maurizio Botta, Rengul Cetin-Atalay, Damla Gozen. “3-Arylideneindolin-2- ones as inhibitors of MDM2-p53 interaction”.

P23 Giedre Valiuliene, Ruta Navakauskiene. “Promyelocytic leukemia therapy possibilities using epigenetic and immunotherapy approach: in vitro and in vivo studies”.

P24 Bastien Cautain, Nuria De Pedro, Javier Pascual, Jesus Martin, Fernando Reyes, Olga Genilloud, Francisca Vicente . ”MDN-0066 a novel inhibitor of VHL/HIF pathway produced by a new Pseudomonas species“.

P25 Milica Pešić, Ana Podolski-Renić, Jelena Dinić, László Kocsis, Antal Csámpai, Szilvia Bősze, and Ferenc Hudecz. “Effects of new quinine- and quinidine epimers in human multi-drug resistant cancer cell lines”.

P26 Deniz Cansen Yildirim, Rengul Cetin-Atalay. “Bioactivities of small molecule kinase inhibitors in liver cancer stem cells ”.

P27 .Ângelo Monteiro, Damla Gozen, Rengul Cetin-Atalay, Maria M. M. Santos “Synthesis of novel spiropyrazoline oxindoles and evaluation as activators of the p53 pathway”.

P28 Dušan Sladić, Barbara Janović, Božidar Čobeljić, Milica Milenković, Miroslava Vujčić, Katarina Anđelković. “In vitro investigation of DNA interactions of square-planar Ni(II) complexes with acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monoden tate pseudohalides”.

P29 Joanna Soin, Monika K. Grudzinska, Ewa Kurzejamska, Krzysztof Bojakowski, Michael H. Lehmann, Hans Reinecke, Charles E. Murry, Cecilia Soderberg-Naucler, Piotr Religa. “Mono cyte Chemoattractant Protein 1–Mediated Migration of Mesenchymal Stem Cells Is a Source of Intimal Hyperplasia”.

P30 Zainab Tayeh, Simon Barak1, Rivka Ofir “Mechanism of Action of Asteriscus Graveolens as an Anticancer Agent”.

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of Athens

3rd Workgroup Meeting COST ACTION CM1106, 26-27 March 2015, Athens, GREECE NCSR Demokritos/ University of Patras/ Agricultural Univ. of AthensP-14

P31 Tamara Todorović, Nenad Filipović, Snežana Bjelogrlić, Katarina Andjelković, Christian D.Muller. ”Effect of metal ion complexation and chalcogen donor identity on pro-apoptotic activity of 2-quinolinecarboxaldehyde chalcogensemicarbazones”.

P32 Didem Karakas, Nazlihan Aztopal, Buse Cevatemre, Veysel Turan Yilmaz, Engin Ulukaya. “Mammospheres (Cancer Stem Cell-Enriched Population) in Breast Cancer Show Resistance Against A Palladium (II)-Saccharinate Complex of Terpyridine”

P33 Vera P. Demertzidou, Alexandros L. Zografos. “Accessing Novel Sesquiterpenoid Families Through Divergent Synthesis in order to Target Cancer Stem Cells”.

P34 Zdenek Wimmer, Uladzimir Bildziukevich, Jana Sustekova, Erkki Kolehmainen, Elina Sievänen ”Betulinic acid amides with spermine in supramolecular chemical biology“.