[About the Cover]Source: Biological Bulletin, Vol. 197, No. 2, Centennial Issue: October, 1899-1999 (Oct., 1999), p.iiPublished by: Marine Biological LaboratoryStable URL: http://www.jstor.org/stable/1542606 .

Accessed: 28/06/2014 12:45

Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at .http://www.jstor.org/page/info/about/policies/terms.jsp

.JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range ofcontent in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new formsof scholarship. For more information about JSTOR, please contact support@jstor.org.

.

Marine Biological Laboratory is collaborating with JSTOR to digitize, preserve and extend access toBiological Bulletin.

http://www.jstor.org

This content downloaded from 193.142.30.23 on Sat, 28 Jun 2014 12:45:27 PMAll use subject to JSTOR Terms and Conditions

Cover

The three-dimensional stereo anaglyph on the cover is a ventral view of a brachiolaria larva of Patiriella regularis, a starfish; the brachiolaria de- picted is about 1500 gum in length. Serotonergic neurons in the larva were stained with a rabbit antiserum and appear, in confocal fluorescent mi- croscopy, as bright dots lining the ciliated bands and brachiolar arms. The image (which should be viewed through the stereo glasses provided with this issue) is composed of 145 optical sections and was reconstructed as described in the article by Francis Chee and Maria Byrne (p. 123).

Immunoreactive serotonergic cells are already visible in the gastrulae of echinoderms; but they increase in number and form an increasingly com- plex neural system as development proceeds. Be- cause the immunoreactivity is associated with the ciliary bands of free-swimming, planktotrophic lar- val forms-as well as with their sensory structures and buccal cavity-the serotonergic system has been thought to coordinate the locomotory and feeding behaviors of these larvae.

In their paper, Chee and Byrne focus on the larval stages of Patiriella regularis, which are all plank- totrophic; thus the development of the serotonergic system can be monitored throughout development, from the gastrula, through the brachiolaria (the last larval stage), and on to metamorphosis. The authors

have used confocal fluorescence microscopy to re- construct the development of the serotonergic ner- vous sytsem in three dimensions and have related the increase in complexity to morphogenetic changes in the larvae. They have demonstrated a complex network of cells with varicose processes that connect the preoral and postoral ciliated bands, supporting the hypothesis that this network is reg- ulating larval feeding and swimming.

In a related article in this issue (see p. 115), Michael Dailey and his colleagues use the mamma- lian brain as a model to show how multidimensional confocal fluorescence microscopy can enhance studies of biological structure and function. The images in this article are fine examples of the tech- niques described, and readers should use the stereo glasses to examine them. This is the third in a series of papers on Concepts in Imaging and Microscopy; the series is supported by the Optical Imaging Association, which has also provided the stereo glasses.

Finally, this issue marks the end of The Biolog- ical Bulletin's first century of publication and the beginning of its second. The four small images on the cover, below the anaglyph, show how the face of the journal changed as the decades passed, biol- ogy expanded, the world shrank, and scientific pub- lishing entered its greatest revolution since the in- vention of movable type. A metamorphosis is certainly at hand, but the nature of the imago re- mains unresolved.

This content downloaded from 193.142.30.23 on Sat, 28 Jun 2014 12:45:27 PMAll use subject to JSTOR Terms and Conditions