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ALL
Bone Marrow and Blood 4 Color Panel Configuration:
TubePurposeMarkers
1Characterize B cell malignancyCD5/CD19/CD45/CD10
2 Identify B cell clonalitykappa/lambda/CD45/CD19+CD20
3Characterize B cell malignancyFMC7/CD23/CD45/CD20
4Identify T cell malignaciesCD7/CD56/CD45/CD3
5Identify T cell and plasma cell
malignanciesCD8/CD38/CD45/CD4
6myeloid differentiationCD15/CD117/CD45/CD34
7myeloid differentiationCD16/CD13/CD45/HLA-Dr
8myeloid differentiationCD11b/Mo2/CD45/CD64
9Characterize B cell malignancy (add on)
cyTdT/CD10/CD45/CD34
10Characterize B cell malignancy (add on)
cyMu/cyCD79a/CD45/CD34
11 DNA ploidy CD45/PI
Patient History: A three year old male presented with
pancytopenia.
.
Sample submitted: A peripheral blood sample was submitted for
flow cytometric
immunophenotyping to rule out acute leukemia. Small blasts with
prominent nucleoli and a high
N/C ratio were seen on the smear. (below)
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50060724.LMD real cells
CD19-PE
FS
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50060724.LMD real cells
CD45-ECD
FS
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CD5/CD19/CD45/CD10
FS vs fluorescence no gate
50060724.LMD real cells
CD10-PC5
FS
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50060724.LMD No Gate
FS
SS
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50060724.LMD real cells
CD45-ECD
SS
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50060724.LMD real cells
CD5-FITC
FS
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50060724.LMD real cells
SS
FS
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The second plot (gated on real cells)
is a side scatter vs CD45 plot. This
plot allows one to distinguish major
cell populations and obtain a "flow
differential". Lymphocytes express
low SS and bright CD45.
Granuolocytes express high SS and
bright CD45. Monocytes express
moderate SS and bright CD45.
Apoptotic cells, erythroid precursors
and plasma cells are negative CD45
and variable SS. Blasts (if present)
are dim to negative CD45 and
variable SS.
Each population as defined by CD45
and SS is painted a distinct color
which is carried throughout the entire
analysis layout for ease of
recognition.
The first plot is a forward scatter (size) vs
sidescatter (granularity/complexity) plot. A
gate is drawn to identify and exclude the
"debris" which could be nonviable or
fragmented cells (low forward scatter),
platelets or other small particles. All
events except debris is denoted as "real
cells".
Population% Classification
Granulocytes5.29ABNORMAL
Lymphocytes38.95NORMAL
Monocytes0.41NORMAL
Blasts48.55ABNORMAL
Other47.90ABNORMAL
The next five plots are forward
scatter (size) vs each individual
marker (fluorescence). This coupled
with the specific populations in
defined colors can provide valuable
information about the proportions
and contents of the sample tube.
Notice the red lymphocyte
population expresses some CD19
(B cell), no CD10 and
predominantly CD5 (T cell)
expression. This along with the
small size identifies a normal
lymphoid population. The
granulocyte and monocyte
populations are larger (high FS) and
negative for CD19 and not CD5.
These serve as internal negative
and positive controls for the
antibodies and control that gating is
set correctly.
The flow differential shows a
significant population of CD45
dim/negative, low side scatter
cells. This predominant
population corresponds with
the blasts seen on the
smear.
There is a decrease in the
granulocyte component.
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50060724.LMD Lymphocytes
CD45-ECD
CD19-PE
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0.00%14.97%0.00%85.03%
50060724.LMD Lymphocytes
CD45-ECD
CD5-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
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2
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3
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4
0.00%68.82%0.00%31.18%
CD5/CD19/CD45/CD10
Gated on Lymphocytes
50060724.LMD No Gate
FS
SS
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50060724.LMD real cells
CD45-ECD
SS
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50060724.LMD Lymphocytes
CD5-FITC
CD19-PE
10
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67.29%1.13%13.77%
Marker (lymph)% lymphs
CD10+0.99 Negative
CD19+ 15.32Predominantly negative
CD5+68.82 Positive
CD19+CD5+1.13 Negative
CD19+CD10+0.77 Negative
50060724.LMD Lymphocytes
CD10-PC5
CD19-PE
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1.84%82.84%0.77%14.55%
50060724.LMD Lymphocytes
CD45-ECD
CD10-PC5
10
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1
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2
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3
10
4
10
0
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2
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3
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0.00%0.99%99.01%
The next five plots show only the cells
in the lymphocyte gate. CD19 is a pan B
cell marker, CD5 is a pan T cell marker
and CD10 is a marker present on some
malignancies. CD19 and CD5 expression
in normal lymphocyte populations show
mutually exclusive populations.
Co-expression of CD19 and CD5 (if
present) is diagnostic for chronic
lymphocytic leukemia (CLL).
The summary of markers in this
tube are gated on the lymphocyte
population. The lymphocytes
express some CD5 and minimal
CD19. CD10 is not expressed.
It is presumed these represent the
residual normal lymphocytes in the
sample.
The first two plots are the gating
plots for this tube.
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50060724.LMD Blasts
CD10-PC5
CD19-PE
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61.79%1.02%37.16%0.03%
50060724.LMD Blasts
CD45-ECD
CD10-PC5
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11.20%87.87%0.02%0.90%
50060724.LMD Blasts
CD45-ECD
CD19-PE
10
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1.83%30.86%5.68%61.63%
50060724.LMD Blasts
CD45-ECD
CD5-FITC
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0.00%0.02%45.90%54.07%
CD5/CD19/CD45/CD10
Gated on dim CD45
Marker % CD45 dim
CD10+99.08Positive
CD19+32.69Partially expressed
CD5+0.02Negative
CD19+CD5+0.01 Negative
CD19+CD10+37.16 Partially expressed
50060724.LMD real cells
CD45-ECD
SS
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50060724.LMD Blasts
CD5-FITC
CD19-PE
10
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0.03%66.18%0.01%33.78%
50060724.LMD No Gate
FS
SS
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The next five plots show only the cells in
the dim CD45 gate and their respective
marking for CD5, CD19 and CD10.
The dim/negative CD45
population (blasts) express the
the B lymphoid markers CD19
(dim) and CALLA marker CD10.
CD19 and CD10 are
coexpressed. The T cell marker
CD5 is not expressed.
The first two plots are the gating
for this tube.
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kappa/lambda/CD45/CD19+CD20
Gating page and FS pattern recognition
50060723.LMD No Gate
FS
SS
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50060723.LMD real cells
CD45-ECD
SS
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50060723.LMD real cells
KAPPA-FITC
FS
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50060723.LMD real cells
CD45-ECD
FS
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50060723.LMD real cells
LAMBDA-PE
FS
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50060723.LMD real cells
CD19+CD20-PC5
FS
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50060723.LMD real cells
SS
FS
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The next five plots are forward scatter
(size) vs each individual marker (FL).
This coupled with the specific
populations in defined colors can
provide valuable information about the
proportions and contents of the sample
tube. In this tube thegreen CD45
negative/dim populaton expresses
CD19+CD20+, without kappa or lambda
light chain expression. There is a
portion of the lymphocytes (red) that
express CD19+CD20+ (pan B cell
marker). There is a significant
lymphocyte cluster that is negative for
CD19+CD20+. It is presumed that
these are the T lymphocytes in the
sample. In addition, a population of
lymphocytes express kappa and
lambda expression.
These serve as internal negative and
positive controls for the antibodies.
The first two plots are the
gating plots
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50060723.LMD real cells
CD45-ECD
SS
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50060723.LMD Lymphocytes
KAPPA-FITC
CD19+CD20-PC5
10
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10
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2
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3
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4
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8.24%
50060723.LMD No Gate
FS
SS
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kappa/lambda/CD45/CD19+CD20
Gated on Lymphocytes
50060723.LMD Lymphocytes
LAMBDA-PE
CD19+CD20-PC5
10
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5.77%
50060723.LMD CD19+ lymphs
LAMBDA-PE
KAPPA-FITC
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57.14%0.51%39.58%
50060723.LMD Lymphocytes
CD19+CD20-PC5
FS
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CD19+ lymphs
Marker % of lymphs
CD19+CD20+15.02Predominantly negative
CD19+CD20+kappa+8.24Predominanly negative
CD19+CD20+lambda+5.77Predominantly negative
Kappa/lambda ratio 1.43Normal
The next four plots show only the cells
in the lymphocyte gate. The
combination CD19+CD20+ are a pan B
cell marker.
An additional gate is placed around
these B cells (painted blue) and the
kappa and lambda expression is
determined on the cells only in this
gate.
The plots can identify a clonal excess
of either kappa or lambda on the
surface of B lymphocytes which
indicates malignancy.
The residual lymphoid population shows
a subset of CD19+CD20+ lymphocytes
which express a normal kappa lambda
ratio.
The first two plots are the
gating plots.
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50060723.LMD Blasts
LAMBDA-PE
KAPPA-FITC
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0.00%99.91%0.04%0.06%
50060723.LMD Blasts
CD19+CD20-PC5
FS
10
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0.00%97.64%2.36%CD19+CD20+
50060723.LMD No Gate
FS
SS
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50060723.LMD real cells
CD45-ECD
SS
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50060723.LMD Blasts
KAPPA-FITC
CD19+CD20-PC5
10
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10
1
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2
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3
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4
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0.41%
50060723.LMD Blasts
LAMBDA-PE
CD19+CD20-PC5
10
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10
1
10
2
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3
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0.48%
Marker (dimCD45 gate)%
CD19+CD20+91.39Positive
CD19+CD20+Kappa+0.41Negative
CD19+CD20+Lambda+0.48Negative
kappa lambda ratio (dim CD45)0.85Normal
50060723.LMD CD19+ blasts
LAMBDA-PE
KAPPA-FITC
10
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10
1
10
2
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0
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0.10%0.18%99.51%0.21%
kappa/lambda/CD45/CD19+CD20
Gated on dim CD45 cells
The first two plots are the gating plots.
The next five plots show only the cells in
the dim CD45 gate and their respective
marking for CD19, CD20, CD19+CD20+
kappa and CD19+CD20+ lambda.
The blast population dimly
expresses the B cell marker
CD19+CD20+. No kappa or
lambda light chain expression
is present.
The first two plots are the
gating plots.
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FMC7/CD23/CD45/CD20
FS vs fluorescence no gate
50060725.LMD No Gate
FS
SS
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50060725.LMD real cells
CD45-ECD
SS
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50060725.LMD real cells
20-PC5
FS
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50060725.LMD real cells
23-PE
FS
10
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50060725.LMD real cells
CD45-ECD
FS
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50060725.LMD real cells
SS
FS
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50060725.LMD real cells
FMC7-FITC
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
The next five plots are forward scatter
(size) vs each individual marker (FL).
This coupled with the specific
populations in defined colors can
provide valuable information about the
proportions and contents of the
sample tube. In this tube , different B
cell markers are used: CD20, CD23
and FMC7. In normal lymphocytes,
one would expect the B lymphocytes
would express CD20, and the CD23
and FMC7 may be partially
expressed. Expression of CD23
and/or FMC7 are helpful in
characterizing B cell malignancies
such as MCL and PLL.
These plots continue to serve as
gating and internal controls.
The first two plots are the gating
plots.
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FMC7/CD23/CD45/CD20
Gated on Lymphocytes
50060725.LMD No Gate
FS
SS
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50060725.LMD real cells
CD45-ECD
SS
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50060725.LMD Lymphocytes
CD45-ECD
20-PC5
10
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0.00%13.69%86.31%
50060725.LMD Lymphocytes
23-PE
20-PC5
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2
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3
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9.13%3.96%86.10%0.80%
50060725.LMD Lymphocytes
FMC7-FITC
20-PC5
10
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10
1
10
2
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3
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4
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1
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2
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41.59%45.32%12.40%0.70%
50060725.LMD Lymphocytes
CD45-ECD
FMC7-FITC
10
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10
1
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2
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3
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4
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0
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1
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2
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90.79%0.00%9.21%0.00%
50060725.LMD Lymphocytes
CD45-ECD
23-PE
10
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3
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2
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96.29%0.00%3.71%0.00%
50060725.LMD Lymphocytes
FMC7-FITC
23-PE
10
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10
1
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2
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3
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8.51%87.60%2.95%0.93%
Marker% lymphs
FMC7+53.98Positive
CD20+13.69Predominantly negative
CD23+3.71Negative
CD20+CD23+0.02Negative
CD23+FMC7+2.95Negative
CD20+FMC7+12.40Predominantly negative
The next six plots show only the cells in
the lymphocyte gate. CD20, FMC 7 and
CD23 are pan B cell markers. Expression
can be used to differentiate B cell
neoplasms such as mantle cell lymphoma,
prolymphocytic lymphoma and chronic
lymphocytic lymphoma.
These residual normal
lymphocytes partially
express the B cell marker
CD20, FMC 7 is
expressed. CD23 is
absent.
The first two plots are the
gating plots.
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FMC7/CD23/CD45/CD20
Gated on CD45dim cells
50060725.LMD No Gate
FS
SS
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50060725.LMD real cells
CD45-ECD
SS
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50060725.LMD Blasts
CD45-ECD
20-PC5
10
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1
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2
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3
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3.06%0.76%90.97%5.21%
50060725.LMD Blasts
23-PE
20-PC5
10
0
10
1
10
2
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3
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4
10
0
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1
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2
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3
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2.78%0.02%97.07%0.12%
50060725.LMD Blasts
FMC7-FITC
20-PC5
10
0
10
1
10
2
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3
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4
10
0
10
1
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2
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3
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0.02%97.63%0.05%2.30%
50060725.LMD Blasts
CD45-ECD
FMC7-FITC
10
0
10
1
10
2
10
3
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4
10
0
10
1
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2
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5.91%93.97%0.06%0.06%
50060725.LMD Blasts
CD45-ECD
23-PE
10
0
10
1
10
2
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3
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4
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0
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1
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2
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3.27%96.67%0.01%0.05%
50060725.LMD Blasts
FMC7-FITC
23-PE
10
0
10
1
10
2
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3
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0
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0.06%99.88%0.00%0.06%
Marker% dim CD45
gate
FMC7+0.12 Negative
CD20+3.82 Negative
CD23+ 0.06 Negative
The next six plots show only the cells
in the dim CD45 gate and their
respective marking for CD20, FMC7
and CD23 Apoptotic cells, erythroid
precursors and blasts will fall into this
gate.
The blasts in the sample do
not express the B cell
markers CD23, CD20 and
FMC 7.
The first two plots are the
gating plots.
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50060726.LMD No Gate
FS
SS
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50060726.LMD real cells
CD45-ECD
SS
10
0
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0
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CD7/CD56/CD45/CD3
FS vs fluorescence no gate
50060726.LMD real cells
CD45-ECD
FS
10
0
10
1
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3
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4
0
256
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50060726.LMD real cells
CD7-FITC
FS
10
0
10
1
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2
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3
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0
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50060726.LMD real cells
CD3-PC5
FS
10
0
10
1
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2
10
3
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4
0
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50060726.LMD real cells
CD56-PE
FS
10
0
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1
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2
10
3
10
4
0
256
512
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50060726.LMD real cells
SS
FS
02565127681024
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256
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The next five plots are forward scatter (size)
vs each individual marker (FL). This coupled
with the specific populations in defined colors
can provide valuable information about the
proportions and contents of the sample tube.
In this tube In this tube, different T and NK
cell markers are used: CD7, CD3 and CD56.
In normal lymphocytes, one would expect the
T lymphocytes to express CD7 and the CD3
in the majority of lymphocytes. CD56 is a NK
marker and is expected to mark a minority of
lymphocytes. These markers are helpful in
assessing T and NK cell malignancies.
These plots continue to serve as gating and
internal controls.
The first two plots are the
gating plots.
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CD7/CD56/CD45/CD3
Gated on Lymphocytes
50060726.LMD No Gate
FS
SS
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768
1024
50060726.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060726.LMD Lymphocytes
CD45-ECD
CD7-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.00%83.03%0.00%16.97%
50060726.LMD Lymphocytes
CD56-PE
CD7-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
69.45%13.58%16.88%0.09%
50060726.LMD Lymphocytes
CD3-PC5
CD56-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
13.13%0.23%17.15%69.48%
50060726.LMD Lymphocytes
CD7-FITC
CD3-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
1.62%67.58%15.30%15.50%
50060726.LMD Lymphocytes
CD45-ECD
CD56-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
86.03%0.00%13.97%0.00%
50060726.LMD Lymphocytes
CD45-ECD
CD3-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
30.54%0.00%69.46%0.00%
Marker% of lymphs
CD7 (T/NK)83.03Positive
CD56 (NK)13.97Predominantly negative
CD3 (T)69.46Positive
CD7+CD56+13.58Predominantly negative
CD56+CD3+0.23Negative
CD3+CD7+67.58Positive
The next six plots show only the cells in
the lymphocyte gate. The pan T cell
markers CD3 and CD7 are expressed in
the majority of the normal lymphocytes.
CD56 is a natural killer cell marker.
Please note CD3 is a lineage specific
marker for T cells, and CD7 expresses T
lymphocytes and NK lymphocytes.
A small proportion of the gated
lymphocytes express the T cell
markers CD3 and CD7. The NK
marker CD56 is predominantly
negative. These represent the
residual normal T lymphocytes in
the sample.
The first two plots are the gating
plots.
13 OF 30
50060726.LMD Blasts
CD7-FITC
CD56-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.01%99.91%0.00%0.07%
50060726.LMD Blasts
CD3-PC5
CD7-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.05%99.91%0.01%0.02%
50060726.LMD Blasts
CD45-ECD
CD7-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
20.03%79.92%0.05%0.00%
50060726.LMD Blasts
CD45-ECD
CD3-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.26%0.28%54.89%44.56%
50060726.LMD Blasts
CD45-ECD
CD56-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.02%0.02%84.50%15.45%
50060726.LMD Blasts
CD7-FITC
CD3-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.02%99.89%0.02%0.06%
50060726.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060726.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
CD7/CD56/CD45/CD3
Gated on dim CD45 cells
Marker% of dim
CD45
CD7+0.05Negative
CD56+0.05Negative
CD3+0.54Negative
The next six plots show only the
cells in the dim CD45 gate and
their respective marking for the T
cell markers CD3, CD7 and and the
natural killer marker CD56. CD7
also marks NK cells and can be
seen in a subset of AMLs.
These markers can assist in
diagnosis of T and NK cell ALL.
The blasts in the sample do
not express the T cell
makers CD7 and CD3; or
the NK marker CD56.
The first two plots are the gating
plots.
14 OF 30
CD8/CD38/CD45/CD4
FS vs fluorescence no gate
50060727.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060727.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD real cells
CD4-PC5
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD real cells
CD45-ECD
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD real cells
CD38-PE
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD real cells
CD8-FITC
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD real cells
SS
FS
02565127681024
0
256
512
768
1024
The next five plots are forward scatter
(size) vs each individual marker (FL).
This coupled with the specific
populations in defined colors can
provide valuable information about the
proportions and contents of the sample
tube. In this tube, different T cell
subset (CD4 and CD8) and the
activation marker CD38 are used In
normal lymphocytes, one would expect
the CD4 and CD8 expression of
lymphocytes to mark mutually
exclusive populations. The sum of the
CD4 + CD8 expression should add up
to the total CD3 population (prior tube).
These markers are helpful in assessing
T cell cell malignancies and identifying
activated cells.
These plots continue to serve as gating
and internal controls.
The first two plots are the gating
plots.
15 OF 30
CD8/CD38/CD45/CD4
Gated on Lymphocytes
50060727.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060727.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD Lymphocytes
CD4-PC5
CD38-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
36.75%36.95%12.68%13.61%
50060727.LMD Lymphocytes
CD8-FITC
CD4-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
40.26%0.41%31.78%27.56%
50060727.LMD Lymphocytes
CD8-FITC
CD38-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
56.61%17.10%14.48%11.81%
50060727.LMD real cells
CD38-PE
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
plasma cells=0.60
Marker (lymph gate)%
CD38+73.70Positive
CD4+40.66Partially expressed
CD8+28.91Partially expressed
CD4+/CD8+ ratio1.46Normal
bright CD38+ (Plasma Cells)0.60Normal
Plasma cells brightly express CD38.
In the FS vs CD38 plot one can
estimate the plasma cell population.
If more than 0.5-1% of the population
is CD38 bright, additional studies
such as CD138, CD56, CDD117,
cytoplasmic Kappa and cytoplasmic
Lambda may be added to further
characterize the plasma cells.
The next three plots show only the
cells in the lymphocyte gate. The
lymphocyte subset markers CD4
and CD8. The activation cell marker
CD38 is assessed on lymphocytes
and the respective lymphocyte
subset.
Plasma cells, if present in the
sample, will express very bright
CD38. In the last plot, we screen for
plasma cells by gating on only
those CD38+ cells that fall into the
fourth decade of fluorescent
intensity.
The gated lymphocytes
express the mutually exclusive
CD4 and CD8 populations The
CD4/CD8 ratio is normal .
These represent the residual
normal T lymphocytes in the
sample.
The activation marker CD38 is
expressed on the lymphocytes.
Plasma cells (bright CD38) are
below 1%.
The first two plots are the gating
plots.
16 OF 30
CD8/CD38/CD45/CD4
Gated on dim CD45 cells
50060727.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060727.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD Blasts
CD45-ECD
CD38-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
14.01%63.87%6.14%15.99%
50060727.LMD Blasts
CD45-ECD
CD4-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.05%0.23%23.22%76.50%
50060727.LMD Blasts
CD45-ECD
CD8-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.00%0.02%36.71%63.27%
Marker% of dim
CD45 cells
CD40.28Negative
CD80.02Negative
CD4/CD8 ratio 11.50 Abnormal
CD4+CD8+0.00Negative
CD38+ 77.87Positive
50060727.LMD Blasts
CD8-FITC
CD4-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.02%99.70%0.00%0.28%
The next four plots show only the
cells in the dim CD45 gate and their
respective marking for CD4, CD8
and CD38
If blasts are present, these markers
can help identify and characterize T
cell malignancies.
The blasts in the sample
express the activation
marker CD38. The T
helper/T suppressor
markers CD4 and CD8 are
not expressed.
The first two plots are the
gating plots.
17 OF 30
CD15/CD117/CD45/CD34
FS vs fluorescence no gate
50060728.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060728.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD real cells
CD45-ECD
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD real cells
34 PC5
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD real cells
15-FITC
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD real cells
CD117-PE
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD real cells
SS
FS
02565127681024
0
256
512
768
1024
The next five plots are forward scatter
(size) vs each individual marker (FL).
This coupled with the specific
populations in defined colors can provide
valuable information about the
proportions and contents of the sample
tube. In this tube, different myeloid and
maturation markers are used: CD15,
CD117, and CD34. These markers are
helpful in assessing myeloid cell
malignancies and determining levels of
cell maturation. Note that the lymphoid
population is negative for these markers,
but that the myeloid population (blue)
expresses the CD15 marker (which
marks more mature myeloid cells such
as granulocytes)
.
These plots continue to serve as gating
and internal controls.
The first two plots are the
gating plots.
18 OF 30
CD15/CD117/CD45/CD34
Gated on Granulocytes (QC)
50060728.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060728.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD Granulocytes
CD45-ECD
34 PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
94.86%0.00%5.14%0.00%
50060728.LMD Granulocytes
15-FITC
34 PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.49%3.18%0.37%95.96%
50060728.LMD Granulocytes
CD117-PE
34 PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
1.35%0.37%98.29%0.00%
50060728.LMD Granulocytes
CD117-PE
15-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
98.53%0.12%1.22%0.12%
50060728.LMD Granulocytes
CD45-ECD
CD117-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
99.63%0.00%0.37%0.00%
50060728.LMD Granulocytes
CD45-ECD
15-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.00%99.02%0.00%0.98%
Marker% granulocytes
CD341.71Predominantly negative
CD1599.02Positive
CD1170.37Negative
This page serves more as a quality
control for the myeloid markers.
Normal granulocytes (blue) will normally
express CD15 and very little CD117 and
CD34. These plots can serve as quality
controls for both gating and antibody
reactivity.
Residual normal granulocytes
are marking as expected.
19 OF 30
CD15/CD117/CD45/CD34
Gated on dim CD45 cells
50060728.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060728.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD Blasts
CD45-ECD
34 PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
14.18%2.64%71.65%11.53%
50060728.LMD Blasts
15-FITC
34 PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
79.68%1.04%19.07%0.20%
50060728.LMD Blasts
CD117-PE
34 PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
83.18%0.50%0.05%
50060728.LMD Blasts
CD117-PE
15-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
1.50%0.02%97.95%0.53%
50060728.LMD Blasts
CD45-ECD
CD117-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
42.83%56.63%0.53%0.01%
50060728.LMD Blasts
CD45-ECD
15-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.83%0.68%44.48%
Marker% blasts
CD3483.18Positive
CD151.52Negative
CD1170.53Negative
The next six plots show only the cells
in the dim CD45 gate and their
respective marking for CD15, CD117
and CD34
If blasts are present, these markers
can help assess myeloid malignancy.
The blasts in the sample
express the stem cell
marker CD34. CD117 and
CD15 are not expressed.
20 OF 30
CD16/CD13/CD45/HLA-DR
FS vs fluorescence no gate
50060729.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060729.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060729.LMD real cells
CD45-ECD
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060729.LMD real cells
HLA-DR-PC5
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060729.LMD real cells
CD16-FITC
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060729.LMD real cells
CD13-PE
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
The next five plots are forward scatter
(size) vs each individual marker (FL).
This coupled with the specific
populations in defined colors can
provide valuable information about the
proportions and contents of the sample
tube. In this tube, different myeloid and
maturation markers are used: HLA-Dr,
CD13, and CD16. These markers are
helpful in assessing myeloid cell
malignancies and determining levels of
cell maturation. Note that the
lymphoid population is negative for
these markers, but that the myeloid
population (blue) expresses the CD13
and CD16 marker (which marks more
mature myeloid cells such as
granulocytes). HLA Dr is a marker of
immaturity and is negative for the
normal granulocytes in this sample.
.
These plots continue to serve as
gating and internal controls.
The first two plots are the gating
plots.
50060729.LMD real cells
SS
FS
02565127681024
0
256
512
768
1024
21 OF 30
50060729.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060729.LMD Granulocytes
CD45-ECD
CD16-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.00%84.22% 15.78%
CD16/CD13/CD45/HLA-DR
Gated on Granulocytes (QC)
50060729.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060729.LMD Granulocytes
CD16-FITC
CD13-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
3.86% 84.79%10.16%
50060729.LMD Granulocytes
CD45-ECD
CD13-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.00%96.17% 3.83%
Marker% of
granulocytes
CD1396.17Positive
HLA DR 3.82 Negative
CD1684.22 Positive
50060729.LMD Granulocytes
CD45-ECD
HLA-DR-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.00%3.82% 96.18%
50060729.LMD Granulocytes
HLA-DR-PC5
CD13-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
90.46%4.32% 0.42%
This page serves as a quality control
for the myeloid markers.
Normal granulocytes (blue) will
normally express CD13 and CD16.
HLA DR is not expressed on mature
granulocytes. These plots can serve as
quality controls for gating and antibody
reactivity.
Residual normal granulocytes
are marking as expected
22 OF 30
CD16/CD13/CD45/HLA-DR
Gated on dim CD45 cells
50060729.LMD No Gate
FS
SS
02565127681024
0
256
512
768
1024
50060729.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060729.LMD Blasts
CD45-ECD
CD16-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
20.17%79.63%0.11%0.10%
50060729.LMD Blasts
CD16-FITC
CD13-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
1.46%0.02%98.33%0.19%
50060729.LMD Blasts
CD45-ECD
HLA-DR-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
12.44%4.74%59.64%23.19%
50060729.LMD Blasts
HLA-DR-PC5
CD13-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
81.62%14.91%2.53%0.94%
50060729.LMD Blasts
CD45-ECD
CD13-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
57.51%42.04%0.42%0.03%
Marker% of dim CD45
CD130.45Negative
HLA DR 84.15Positive
CD160.11 Negative
The next five plots show only the cells in the
dim CD45 gate and their respective marking
for HLA Dr, CD13 and CD16
If blasts are present, these markers can
help assess myeloid malignancy and
immaturity.
The blasts in the sample
express HLA DR. The myeloid
markers CD13 and CD16 are
negative.
The first two plots are the gating
plots.
23 OF 30
CD11b/MO2/CD45/CD64
FS vs fluorescence no gate
50060730.LMD No Gate
FS
SS
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1024
50060730.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060730.LMD real cells
CD11b-FITC
FS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060730.LMD real cells
Mo2-PE
FS
10
0
10
1
10
2
10
3
10
4
0
256
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768
1024
50060730.LMD real cells
CD45-ECD
FS
10
0
10
1
10
2
10
3
10
4
0
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1024
50060730.LMD real cells
CD64-PC5
FS
10
0
10
1
10
2
10
3
10
4
0
256
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1024
%
50060730.LMD real cells
SS
FS
02565127681024
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256
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768
1024
The next five plots are forward scatter
(size) vs each individual marker (FL).
This coupled with the specific
populations in defined colors can
provide valuable information about the
proportions and contents of the
sample tube. In this tube, different
myeloid/monocytoid markers are
used: CD11b, CD64, and Mo-2.
These markers are helpful in
assessing or differentiating
myeloid/monocytoid cell
malignancies. Note that the lymphoid
population is negative for these
markers, but that the myeloid
population (blue) and monocyte
population (purple) expresses
CD11b. The CD64 and Mo-2 are only
expressed on the monocyte
population.
.
These plots continue to serve as
gating and internal controls.
The first two plots are the gating
plots.
24 OF 30
CD11b/MO2/CD45/CD64
Gated on Granulocytes (QC)
50060730.LMD No Gate
FS
SS
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1024
50060730.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
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1024
50060730.LMD Granulocytes
CD45-ECD
CD64-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
34.25%0.00%65.75%0.00%
50060730.LMD Granulocytes
CD45-ECD
CD11b-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
3.88%0.00%96.12%0.00%
50060730.LMD Granulocytes
CD64-PC5
Mo2-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
63.75%36.17%0.08%0.00%
50060730.LMD Granulocytes
CD45-ECD
Mo2-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
99.81%0.00%0.19%0.00%
50060730.LMD Granulocytes
CD11b-FITC
CD64-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
35.57%0.90%58.44%5.09%
50060730.LMD Granulocytes
Mo2-PE
CD11b-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.04%4.60%0.04%95.33%
Marker% of
Granulocytes
CD11b96.12Positive
CD6465.75Positive
Mo2 (monos)23.05Partially expressed
These plots serve as a quality control
for granulocyte and monocyte markers.
Normal granulocytes (blue) will
normally express CD11b and CD64.
Mo2 is expressed only on monocytes,
but not on granulocytes. These plots
can serve as quality controls for gating
and antibody reactivity.
50060730.LMD Monocytes
CD64-PC5
Mo2-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
2.34%23.05%47.66%
This plot serve as a quality
control for Mo 2 and CD64.
As shown monoctes are
positive for these two
markers.
Residual normal granulocytes
are marking as expected.
25 OF 30
CD11b/MO2/CD45/CD64
Gated on dim CD45 cells
50060730.LMD No Gate
FS
SS
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1024
50060730.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
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1024
50060730.LMD Blasts
CD45-ECD
Mo2-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
29.61%70.29%0.05%0.05%
50060730.LMD Blasts
CD45-ECD
CD11b-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
2.81%97.04%0.06%0.10%
50060730.LMD Blasts
CD64-PC5
CD11b-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
1.17%98.57%0.02%0.24%
50060730.LMD Blasts
CD45-ECD
CD64-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
24.36%74.32%0.39%0.94%
50060730.LMD Blasts
Mo2-PE
CD64-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.14%99.60%0.00%0.26%
50060730.LMD Blasts
Mo2-PE
CD11b-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.14%99.75%0.00%0.11%
Marker% of dim CD45
CD11b0.16Negative
CD641.32Negative
Mo20.10Negative
The next six plots show only the cells in
the dim CD45 gate and their respective
marking for the myeloid and monocytoid
markers CD11b, CD64 and Mo-2
If blasts are present, these markers can
help assess and differentiate myeloid and
monocytoid malignancy .
The blasts do not express the
myeloid/monocytoid markers
CD11b, CD64 and Mo2.
The first two plots are the
gating plots.
26 OF 30
50060746.LMD Blasts
CD45-ECD
cy79A-PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
7.99%86.62%0.69%4.69%
50060746.LMD Blasts
CD45-ECD
cyMu-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
3.14%0.20%95.01%1.65%
50060746.LMD No Gate
FS
SS
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50060746.LMD real cells
CD45-ECD
SS
10
0
10
1
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2
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3
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4
0
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50060746.LMD Blasts
cy79A-PE
CD34-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
1.59%68.65%3.20%26.56%
50060746.LMD Blasts
cy79A-PE
cyMu-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
0.43%4.49%5.05%90.02%
50060746.LMD Blasts
CD34-PC5
cyMu-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
2.74%1.49%28.11%67.66%
50060746.LMD Blasts
CD45-ECD
CD34-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
6.21%66.24%2.11%25.44%
cyMu/cyCD79a/CD45/CD34
Gated on dim CD45 cells
The next six plots show only the cells in
the dim CD45 gate and their respective
marking for the cytoplasmic Mu,
cytoplasmic CD79a and CD34. CD79a is
a lineage specific marker for B lymphoid
cells. cyMu identifies a subset of B
lymphoid cells.
If blasts are present, these markers can
help assess and differentiate B lymphoid
cells. .
The blasts express the B
lymphoid marker cyCD79a and
CD34. The cytoplasmic Mu is
not expressed.
Marker% of dim CD45
cyMu3.34 Negative
cyCD79a94.62 Positive
CD3472.45 Positive
27 OF 30
50060752.LMD Blasts
CD45-ECD
CD10- PE
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
9.50%85.53%0.60%4.36%
50060752.LMD Blasts
CD45-ECD
cyTdT-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
11.89%78.54%1.79%7.77%
50060752.LMD No Gate
FS
SS
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50060752.LMD real cells
CD45-ECD
SS
10
0
10
1
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2
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3
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4
0
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50060752.LMD Blasts
CD10- PE
CD19-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
2.27%80.81%2.87%14.05%
50060752.LMD Blasts
CD10- PE
cyTdT-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
3.23%86.07%2.99%7.71%
50060752.LMD Blasts
CD19-PC5
cyTdT-FITC
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
13.87%66.41%8.61%11.12%
50060752.LMD Blasts
CD45-ECD
CD19-PC5
10
0
10
1
10
2
10
3
10
4
10
0
10
1
10
2
10
3
10
4
15.54%63.54%3.83%17.10%
cyTdT/CD10/CD45/CD19
Gated on dim CD45 cells
The next six plots show only the cells in
the dim CD45 gate and their respective
marking for the cytoplasmic TdT, CD10
and CD19. TdT marks B lymphoblasts
(and some myeloblasts). CD10 marks the
CALLA (common acute lymphoblastic
leukemia antigen)
If blasts are present, these markers can
help assess and differentiate
lymphoblastic leukemia. .
The blasts express TdT, CD10
and CD19. TdT is coexpressed
on both the CD19 and CD10
cells.
Marker% of dim CD45
cyTdT90.44 Positive
cyTdT+CD10+86.07 Positive
cyTdT+CD19+66.41 Positive
28 OF 30
50060724.LMD real cells
CD45-ECD
SS
10
0
10
1
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2
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3
10
4
0
256
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1024
50060723.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060725.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060726.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060727.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
768
1024
50060728.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
512
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1024
50060729.LMD real cells
CD45-ECD
SS
10
0
10
1
10
2
10
3
10
4
0
256
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1024
50060730.LMD real cells
CD45-ECD
SS
10
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Tube 1-8 SS vs CD45 pattern QC
Tube #Total eventsReal
cellsDebrisGranulocytesLymphocytesBlasts
127238258335.165.2938.9548.55
226769254185.055.1539.0333.50
327579262414.855.3738.3731.62
427733262785.255.8038.9130.73
527702261695.535.5938.6340.08
626966256085.043.1939.8648.55
71872881777055.125.3138.3333.42
854186513915.165.1637.4833.08
This page displays the CD45 vs
SS plots for each of the 8 tubes of
the panel. review of the number of
events counted in each tube and
the placement and proportions of
each of the populations serve as a
QC parameter.
29 OF 30
50060731.LMD lymphocyteDNA
D N A
Count
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50
75
100
# of events = 10818
50060731.LMD singlicates
CD45 LOG
SS
10
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10
1
10
2
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3
10
4
0
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Cell cycle tube
50060731.LMD No Gate
D N A
RATIO
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singlicates
50060731.LMD blastDNA
D N A
Count
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50
75
100
# of events =45463
50060731.LMD singlicates
D N A
Count
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100
200
300
400
# of events = 59795
DNA ploidy and S-phase fraction can
be a useful tool in assessing
hematologic malignancies.
The first plot is the ratio of peak vs
area, of the FL3 linear cell
fluorescence as it traverses the laser
spot. We have gated out any doublets
that may interfere with the analysis.
Doublets are cells that stick together
and give a false signal of twice the DNA
content.
The second plot allows us to select
different populations to assess the DNA
content. DNA content of the singlicate
gat includes both the normal
lymphocytes (that serve as a diploid
control) and the abnormal population.
The subsequent single parameter
histograms assess the DNA histogram
of the selected populations. In this case
all population show a normal diploid
histogram. Accurate S-phase can only
be assessed in cases with > 20,000
events.
The "flow differential" in this
specimen shows a bright CD45
(normal ymphoid population) and a
dim CD45 (blast) population.
Additional DNA studies using an
appropriate antibody in FITC (CD19
or CD20) vs DNA can provide more
specific data.
In this case the normal
lymphocytes serve as the diploid
control. The blasts are aneuploid
(hypodiploid). The S-phase fraction
appear high. Further analysis is
need to determine DNA statistics.
DNA index 0.53Aneuploid
30 OF 30
ParameterLymphocyte
gate
(dim/neg)
CD45 gate
Granulocyte
gate
CD19+CD20+17.11Pred. Neg91.39PosNANA
CD19+CD20+kappa+ 8.24Pred. Neg0.41 NegNANA
CD19+CD20+lambda+5.77Pred. Neg0.48NegNANA
kappa/lambda ratio1.43Normal0.85NormalNANA
CD10+2.60Neg99.08 PosNA NA
CD19+14.90Pred. Neg32.69 PartialNA NA
CD5+68.82Pos0.02 NegNA NA
CD19+CD10+0.77Neg37.16PartialNA NA
CD19+CD5+1.13Neg0.01NegNA NA
FMC7+53.98Pos0.07NegNA NA
CD20+13.69Pred. Neg3.82NegNA NA
CD23+4.77Neg0.14NegNA NA
CD7+83.03Pos0.04NegNA NA
CD56+13.67Pred. Neg0.07NegNA NA
CD3+69.20Pos0.06NegNA NA
CD3+CD7+67.58Pos0.01NegNA NA
CD4+40.66Partial0.28NegNA NA
CD8+27.97Partial0.02NegNA NA
CD4/CD8 ratio1.46Normal11.50 AbnormalNA NA
CD38+73.70Pos77.87PosNA NA
CD38+ bright (PC)0.60NormalNA NANA NA
CD4+CD8+0.41 Neg0.00NegNA NA
CD11b+NA NA0.16Neg96.12Pos
CD64+NA NA1.19Neg65.75Pos
Mo2+NA NA0.10Neg0.08Neg
CD13+NA NA1.48Neg94.95Pos
HLA DR+NA NA84.15Neg3.82Neg
CD16+NA NA0.21Neg88.65Pos
CD34+NA NA83.18Pos1.71Neg
CD15+NA NA1.52Neg99.14Pos
CD117+NA NA0.55Neg0.37Neg
Population%
Granulocyte5.29Abnormal
Lymphocyte38.95Normal
dim CD45 cells (apoptotic cells or blasts) 48.55Abnormal
Monocyte0.41Normal
negative CD45 cells (apoptotic cells, erythroid
precursor, blasts, or plasma cells)
47.90Abnormal
Summary of Statistics
This page is a summary of the
statistics on this case.
A dim/negative CD45, low SSC
population expresses the B
lymphoid markers CD19 (dim)
and CD10. HLA Dr, CD34 and
CD38 are also expressed. CD20,
CD23, FMC-7 nor kappa/lambda
light chains are expressed.
Myeloid, monocytoid and T
lymphoid markers are not
expressed.
Additional studies showed
cytoplasmic TdT, cytoplasmic
CD79a are also expressed on the
blasts.
This is most consistent with a
Pre-B Acute lymphoblastic
leukemia (ALL)
DNA content of the blasts is
aneuploid (hypodiploid) with a
high S-phase fraction.
The lymphocyte population
expresses a normal phenotype,
predominantly T cells with a
normal CD4/CD8 and B cells with
a normal kappa/lambda ratio.The
granulocytes in the sample
express a normal phenotype. This
serves as an internal control.
The "flow differential" identifies a
predominant dim/negative CD45
low SSC population, and a
decreased granulocyte
population. The proportion of
lymphocytes is normal.
