Biotechnology Biotechdesk Catalog 2006 07

CONTENTS

� DNA/ RNA PURIFICATION ............................................... 1

Nucleic Acid Isolation

� PCR ............................................................................................5

Polymerase Chain Reaction, PCR Optimization,

Polymerases, Real Time PCR, DNTPs / Ladders /

PCR Enhancer / Buffers, Custom Primer /

Oligo Synthesis & Modifications

� RT-PCR .................................................................................. 14

Reverse Transcriptases, Kits for RT-PCR/Realtime RT-PCR

� CLONING .............................................................................. 16

PCR Cloning, CopyControl Cloning Systems,

BAC Cloning, Cosmids & Phage Extracts,

Screening and Electroporation, Competent Cells

� cDNA LIBRARIES AND CLONES ................................... 25

Ready Clones, cDNA/BAC Clones,

� IN VITRO TRANSCRIPTION .......................................... 29

Prokaryotic Polymerases, Probe synthesis,

RNA Amplification, Transcripts for Translation,

� MICROARRAY ..................................................................... 33

Selection guide and

Related Items, Amplification Kits

� RNAi ....................................................................................... 36

shRNA Libraries, Vectors, Transfection kits, Making shRNA

� MODIFYING ENZYMES ................................................... 38

Nucleases, Glycosylases, Phosphatases, Kinases/Ligases,

DNA - binding proteins, Restriction Enzymes

� TRANSPOSOMICS ............................................................. 50

Insert Promoters, Origin of replication, Plasmid Rescue,

Insert Signal Peptide, Custom Transposons,

In-vivo mutagenesis, Protein Engg.

� SEQUENCING ...................................................................... 55

Transposon Insertion kits,

Kits for automated & manual Seq

� PROTEIN PURIFICATION ............................................... 57

Bacterial lysis, Affinity Chromatography

� INSTRUMENTS ................................................................... 59

Electrophoresis, Electro blotting, Centrifuges, Cryogenics,

Homogenizers, Mixers, Stirrers, Shakers, Water Baths,

Hybridization Ovens, Electroporation Cuvettes,

Transilluminators, Crosslinkers, General Lab Equipments

� X-RAY CRYSTALLOGRAPHY ........................................ 77

Crystal Screens, Cystal Optimization,

Heavy Atom Derivatization, Consumables,

Cryo Crystallization

� ENZYME DETECTION REAGENTS AND

ASSAY KITS ......................................................................... 83

MMPs, Viral Proteases, Proteases, Glycosidases,

Phosphatases, Phospholipases, Galactosidase,

β-secretase, Peroxidases-redox Enzymes,

Cytochrome P450 & Oxidases, Amyloids, Apoptosis

� SIGNAL TRANSDUCTION ............................................... 97

GTP-Ras, GTP-Ran, GTP Heterotrimer,

GAP, GTPase, GPCRs, Importins, P13K, PTK,

PKA, PKB, PKC, CamK, Cyclin dep.

Kinase, Substrates, Inhibitors, Phosphatases,

� ANTIBODIES ..................................................................... 113

*Primary, Secondary Substrates, Biotin-Avidin

� RECOMBINANT PROTEINS ........................................ 116

Prion Proteins, Viral Proteins, Transcription Factors,

Hematopoetins, Nuclear Receptors, Cytokines,

Growth Factors

� PEPTIDE SYNTHESIS ................................................... 127

Amino acids, Resins and reagents, Building Blocks,

Catalogue Peptides

� CUSTOM SERVICES ....................................................... 131

Genomic & Proteomic Services,

Antibody and Other Services

� FLUORESCENCE ............................................................. 134

Fluorescent Dyes, Probes, FRET Probes, Quenchers,

Protein detection

Calcium Detection, Cell Viability,

Proliferation & Toxicity,

� CYTOGENETICS .............................................................. 149

StarFish Chromosomal Paints,

� NEW TECHNOLOGIES .................................................. 151

APA Gene Technology,

Cloning Vector,

Eucaryotic Expression System

� INDEX ..................................................................... (i) - (viii)

� www.biotechdesk.com � T : 040-27017477 / 65916167 F : 040-27016168 � [email protected]

Simple, rapid miniprep-type procedure - Scalable to larger volumesHigh yields of highly purified plasmid DNA of sequencing qualityUses no toxic organic solvents

DNA PURIFICATIONDNA PURIFICATIONPlasmidMAX™ DNA Isolation Kit

Purify genomic DNA up to 10 µg from as little as 325 µl of bloodHigh DNA purity- A ratios of 1.8-2.0 - DNA is PCR ready260/280

No organic solvents, no columns

MasterPure™ DNA Purification Kit for Blood

Versatile- recover DNA from leaves of many plant speciesRapid- purify DNA in less than 1 hourRecover DNA of higher integrity than column-based purification methods.Takes care of PCR inhibitors like polyphenolics and polysaccharides

MasterPure™ Plant Leaf DNA Purification Kit

Rapid-extract PCR-ready soil / water high M.W. DNA in less than 45 minutesNo need for dedicated equipment or bead beating or organic reagentsRemoves PCR inhibitors like humic and fulvic acids

SoilMaster™/ WaterMaster™ DNA Extraction Kit

Extensive sample range- purify DNA from any sample, every timeHigh purity- O.D. ratios consistently between 1.8 and 2.0260/280

High yields, High sensitivitySafe- no phenol, chloroform, or other caustic solvents

MasterPure™ DNA Purification Kit

Higher yields of yeast chromosomal DNA than other kitsVersatile- recover DNA from a wide variety of yeast species Purify DNA in less than 40 minutesEliminates bead beating, enzymatic lysis and organic extractions

MasterPure™ Yeast DNA Purification Kit

Kits for DNA Extraction and Purification from EPICENTRE

Biotech Desk Pvt. Ltd. Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: [email protected]

CUSTOM GENE SYNTHESISCUSTOM GENE SYNTHESISCUSTOM GENE SYNTHESISWhy waste time cloning and sequencing your gene when we do it all for you!

Gene synthesis at SPECIAL PRICES

Includes insertion into vector of choice

Includes expression optimization

Includes DNA sequencing data plus chromatogram

100% fidelity GUARANTEED

Lyophilized plasmid DNA provided

E.coli culture transformed and stab provided at minimal cost

Expression systems: Bacterial (E.coli), Yeast (Pichia pastoris)

Baculovirus (Insect Cell), Mammalian (Mouse)

We guarantee a quick turnaround-time, unbeatable pricing and large inserts!

BAC LIBRARY CONSTRUCTIONBAC LIBRARY CONSTRUCTIONOTHER GENOMIC SERVICESOTHER GENOMIC SERVICESPlasmid library construction

Lambda library construction

Site-directed mutagenesis

Large-construct DNA engineering

APA genome walking service

Subcloning

Gap filling

5' and 3' RACE of first-strand cDNAs

5' and 3' genomic walking

Identifying intron/exon junctions

Identifying transgene/genomic DNAjunctions

Identifying gene traps and transposon-insertion location

We guarantee coverage

We perform HMW DNA extractionat our facilities

We have experience with plant,fungal, bacterial and animal libraries

We have extensive experience withGC-rich organisms and marine bacteria

Our arrayed BAC libraries areshipped in bar-coded microplates

Shipment temperature of non-arrayedlibraries is always monitored usinga BLUE FLAG Temperature Data logger

We provide technical support

Biotech

Biotech Desk Pvt. Ltd.Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: [email protected]

NAR TA EU E

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100%CUSTOMER

SATISFACTION

Biotech Desk Pvt. Ltd.4F 5 Ballad Estate, Tarnaka, Hyderabad-500 017,India.

Tel: +91-40-27017477 / 65916167 Fax: +91-40-27016168 Email: [email protected]

Just identify your clone from:Nucleotide GenBank Accession #Gene symbols

Clone ID (cDNA only)Yeast ORF ID, Record #

Millions of Clones

We can ship them as glycerol stocks or on Isocode paper

Mammalian cDNA Librariesand Clones

Mammalian Gene Collection (MGC) Assay-Ready MGC cDNA BioTrack Human Gene SubsetsHuman ORF CollectionHuman Freedom ORFsIncyte Gene Collection (IGC)Mammalian IMAGE ESTsOther ESTsBrain cDNA LibrariesIncyte cDNA Libraries

Non Mammalian cDNALibraries and Clones

Brucella ORF Collection C. jejuni ORF CollectionZebrafish cDNA LibraryZebrafish Gene Collection (ZGC)Zebrafish IMAGE cDNAsZebrafish IMCB, Morpholino andNEIBank cDNA Clones CollectionsDrosophila Gene Collection (DGC)Xenopus IMAGE cDNAsC. elegans ORF, ORF-RNAi andPromoter Collections

Candida albicans Genomic LibraryHA-Tagged Yeast StrainsYeast Insertional Mutant StrainsYeast Knockout StrainsYeast Magic Marker StrainsYeast ORF CollectionYeast TAP Fusion CollectionYeast Tet-Promoter Hughes Strains

Yeast cDNA Librariesand Clones

BAC ClonesFISH Mapped BAC ClonesGenMap Human BAC CollectionDisease-specific Human MappedBAC CollectionsMere Mouse Mapped BAC CollectionCalTech Human and MouseBAC Clones

Biotech Desk Pvt. Ltd.4F 5 Ballad Estate, Tarnaka, Hyderabad-500 017,india

Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: [email protected]

PEPTIDE SYNTHESISCUSTOM

From 5 mg to 100mg and more

For purity levels ranging between 70% to >98%

N-terminal and C-terminal modifications

Conjugation to carrier

Peptides for raising antibodies

GMP Grade peptides

Labelled peptides with fluorophore

Phosphopeptides

Glycopeptides

Lipopeptides

Cyclic Peptides

DNA-peptide conjugates

Drug-peptide conjugate

ANTIBODY PRODUCTIONCUSTOM

Polyclonal AntibodiesNIH 90-day standard protocol or

70-day conventional protocol

10 ml pre-immune serum

100 ml anti-serum

ELISA

Peptide synthesis 10 mg peptide,

>80% purity, MS and HPLC certified

Peptide-Carrier protein conjugation

to BSA or KLH

Monoclonal AntibodiesFree consultation for

epitope selection

Evaluation of screening methods

Frequent interactions between

You & Biotechdesk during hybridoma

development process

Project segmentation into different

phases to provide flexibility

for terminating the project at the

end of each phase

Biotech Desk Pvt. Ltd. Tel: +91-40-27017477/ 65916167 e-mail: [email protected]

Additional antibody services include:

Biotech Desk Pvt. Ltd4F 5 Ballad Estate,Tarnaka, Hyderabad 500 017 IndiaTel: +91-40-27017477/ 65916167 Fax: +91-40-27016168Email: [email protected]

Biotech

a i e o i s fgg d ar & A o e p u

N t v pr te n , A finity-ta e v iants ss ciat d rod cts

GTP Family of Signaling ProteinsSmall GTPases (Ras Superfamily)

Heterotrimeric G-Proteins

GEFs (Guanine Nucleotide Exchange Factors)

GAPs (GTPase Activation Proteins)

GTPase Cycle Effectors

GTPase Modifying Enzymes

GPCRs (G-Protein Coupled Receptors)

Importins

Phosphoinositide 3-kinase (PI3K) PI3K a

PI3K b

PI3K g

PI3K d

Adaptor Proteins

Substrates

Inhibitors

Antibodies

Tyrosine KinasesAbl, Hck, Lck, Src, PDGFR, Raf

Serine/Threonine KinasesMEK, MKK

Protein Kinase A, B and CProtein Kinase A

AkT/ PKB

PKC-a, PKC-b, PKC-g, PKC-d, PKC-e

Inhibitors

Antibodies

Substrates

Other Related Proteins:Calcium/ Calmodulin-dependent

kinases

Cyclin dependent Kinases

Phosphatase

Transcription Factors

Biotech Desk Pvt. Ltd.Tel: +91-40-27017477 / 65916167 Fax: +91-40-27016168Email: [email protected]

Macromolecular CrystallographyThe JBS Product Family- the key to protein structure determination

JBScreen Classic bulk10 individual kits, 24 conditions each, supplied in 10 ml tubes

JBScreen Classic ampoules10 individual kits, 24 conditions each, supplied as 0.7 ml single shots in a vial

JBScreen Classic refillRefill your JBScreen ampoules hits

JBScreen Classic HTS2 pre-filled 96 deep-well Blocks

TMWizard Screens I & II from Emerald BiosystemsHighly effective random sparse matrices for the crystallization of biologicalmacromolecules

JBScreen MembraneCover 72 of the most promising reagents for crystallization of membrane proteins

JBScreen CryoHas been designed for vapour diffusion set-ups to determine initial crysallization conditions

JBScreen Cryo ProProduce effective cryo-protectants from your crystallization reservoir solution

JBScreen PlusMost useful in the optimization of preliminary crystallization conditions

JBScreen Detergents kitsAre ideal supplements to the JBScreen Membrane screens

JBS Halo KitsHalogenated ATP and GTP analogs provide an alternative method that allow incorporation of heavy atoms into a large number of physiologically relevant enzymes.

Ask us for plates and accessories for crystallization:Linbro plates, CombiClover Plates, Compact Clover plates,Sealing Tape, Cover Slides and Grease BiotechBiotech Desk Pvt. Ltd. Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: [email protected]

Crystallization ScreensWhat makes JBScreeen Classic so different from other crystal screens available?

The composition of the buffers in the screen cover 240 of the most prominent reagent mixtures for protein

crystallization. Their composition results from data mining several thousands of crystallized proteins.

ANTIBODIES FOR EVERYTHING!! ANTIBODIES

Secondary Antibodies:

Epitope Tagged Antibodies

Substrates

Tagged to Biotin, HRP, Alkaline Phosphatase, FITC, Texas Red, Cy3, PE, Cy5 or Gold

Anti-His, Anti-HA, Anti-GST, Anti-GFP, Anti-cMyc and more

OPD, TMB, DAB, Urea Peroxide, BCIP/NBT

Antibodies for: = Cancer

= Neuroscience

= Signal transduction

= Cell cycle regulators

= Apoptosis

= Cytokines

= MMPs

= Viruses

Antibody Related Kits:= KLH Conjugation

= BSA Conjugation

= Maleimide-activated Antigen Conjugation

= Alkaline Phosphatase Assay

= Peroxidase ELISA Assay

= Biotin Labelling

= Biotin, Streptavidin and its conjugates

If you cannot find what you are looking for, we will custom make the antibody for you!!

Try our polyclonal and monoclonal custom antibody production services

Biotech

Biotech Desk Pvt. Ltd.Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: [email protected]

Phosphospecific antibodies are available in biotinylated and dye-labeled forms, as well as

AP/ HRP and APC/ B-PE/ R-PE conjugates, Biotechdesk also provides custom phosphospecific

Antibody production; please contact [email protected] for more information.

Biotechdesk offers a large selection of phosphospecific antibodies including:

In Need of Phosphospecific Antibodies?

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UNBEATABLE PRICE

COMPATIBILITYThe cuvettes are compatible with most electroporation systems

LOW DEAD VOLUMESAll 1mm and 2mm cuvettes have a tapered V bottom so that reduced sample volumes can be used while aiding sample pick up and minimizing dead volumes.

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BACMAX and FosmidMAX DNA Purification Kit

Superior yields- alkaline lysis method

Convenient- No columns, no organic extractions

Versatile- Protocol can be scaled up from 1.5 ml to 100 ml

GENOMIC CLONINGGENOMIC CLONINGGENOMIC CLONING

CopyControl™ Fosmid Library Production

= Cloning-ready pCC1FOS or pCC2FOS vector

coupled to ultra high efficiency MaxPLax

Lambda packaging extract

= No need for restriction enzyme digestion or

PFGE to prepare DNA

= High cloning efficiencies

Maximise yields from every clone!!

CopyControl™ BAC Library Production

= Linearized, dephosphorylated and highly

purified pCC1 BAC vector

= Fast Link DNA Ligase which reduces ligation

times from 16h to 4 h

= Unique colony screening process for

estimating size of the BAC clones without

restriction digestion or PFGE

Construct BAC or Fosmid Libraries in single copy and induce to High Copy number!

Electroporation CuvettesElectroporation CuvettesElectroporation CuvettesOur range of HiMaX Electroporation cuvettes are designed to maximise molecular electroporation and electrofusion efficiencies for Bacteria, Yeast, Insect, Plant and Mammalian cells.


Optimizing Real-Time PCR

Convenient, simple & easy to usePreMixes are preoptimized to save you time and

energy.

Simple OptimizationRobust enzyme mix and set of 2X optimization

PreMixes contains all necessary components

necessary for successful PCR. Enables optimum

amplification efficiency to generate high quality,

trustworthy data.

Consistent resultsEnzyme and PreMix formulations do not change

which gives you consistent, error-free results

every time.

No hot-start neededFailSafe™ PROBES Real-Time PCR System is

compatible with all real-time PCR instruments and

fluorescent probes.

Using Labeled ProbesFailSafe™ PROBES Real-Time PCR Optimization Kit

FailSafe™ PROBES Real-Time PCR PreMix-Choice Kit

Using SYBR Green I DyeFailSafe™ GREEN Real-Time PCR Optimization Kit

FailSafe™ GREEN Real-Time PCR PreMix-Choice Kit

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Extremely high sensitivity and specificity.®

Easy-to-use PreMix contains all components for successful quantitative RT-PCR, including SYBR Green I dye.

A thermostable enzyme increases sensitivity and specificity by reducing RNA secondary structure.

MasterAmp PCR Enhancer increases sensitivity and specificity by reducing polymerase pausing and stops during reverse transcription and PCR.

®SYBR Green I dye for detection saves time and expense compared to use of labeled primers.

The kit uses flexible protocols for real-time detection on almost all real-time PCR thermocyclers

FIRST TIMEPerform real-time PCR with your template & primers using the FailSafe GREEN Real-time PCR Optimization Kit & select the optimal Real-time PCR Premix

And EVERY TIMEFor consistent PCR results, choose the PCR Premix identified as optimal when you get the FailSafe GREEN Real-time PCR Premix Choice Kit

· Real time PCR Premixes contain all

components for quantitative PCR using Sybr

Green I dye.

· No specific probes required.

· Includes ROX, an internal reference dye.

· Kits available for both tube and capillary

instruments

Realtime RT-PCRMasterAmp™ GREEN Real-Time RT-PCR Kit The MasterAmp™ GREEN Real-Time RT-PCR Kit provides

all necessary components to perform high-sensitivity one-step ®quantitative T-PCR using SYBR Green I Dye for detection.


™ FailSafePCR System

™ FailSafePCR System

™FailSafe

PCR SystemWhatever the length, whatever the sequence, the FailSafe™ PCR System will faithfully amplify your template every time!

Applications l Amplify any template up to 20 kb

l Amplify templates up to 85% GC rich l High sensitivity amp. of as little as 1 copy of template

l High fidelity amplification l Multiplex PCR

l No need for hot start

I STF R TIMEe m R t

P rfor PC with your templa e and i r ne

pr me s usi g the FailSaf ™ PCR r Mi l ct on t d P e x Se e i ki an choose the r M w t b t i i .P e ix ith he es ampl f cation

And EVERY TI EMGe t e se ec e PreMix with the

t h l t d FailSafe™ PCR S st m and u e it or y e s fconsistent mp ification of you

a lrtemplate primer pair./

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High sensitivity RT-PCR from as little as 1 pg of total cellular RNA

Out-performs similar products from other vendors

Reduces effect of RNA secondary structure by performing RT reactions at higher temperature

Reduces chance for cross contamination by using one enzyme in a one-step and one-tube reaction protocol with no sample transfer

MasterAmp PCR Enhancer Technology enhances PCR performance with GC-rich and other difficult templates

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Highly accurate RT-PCR amplification of RNA templates for

Cloning Sequencing Expression Transcription analysis

All purpose RT-PCR in which full-length amplification and high accuracy are essential

Multiplex RT-PCR

Comes along with an TAQURATE, a high flidelity polymerase enzyme blend

MasterAmp™ High Sensitivity RT-PCR Kit MasterAmp™ High Fidelity RT-PCR Kit

Never-ending reasons why you should buy the MonsterScript™ 1st-Strand cDNA Synthesis Kit

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MonsterScript™ Reverse Transcriptase lacks RNase H, enabling improved synthesis of full-length cDNA even for long mRNA.

MonsterScript is thermostable, permitting reverse transcription at temperatures >50°C, reducing RNA secondary structure& improves priming specificity.

The kit includes both an oligo(dT)-containing and a random nonamer primer.

A potent RNase Inhibitor is included.

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MonsterScript™ RT PreMix contains optimized concentrations of dNTPS, Mg+2 and Betaine for superior performance & minimal pipetting steps.

Betaine in the cDNA Synthesis PreMix reduces pausing and stops during reverse transcription.

First-strand cDNA can be made from picogram amounts of total RNA.

The cDNA is a good template for subsequent end-point PCR or real-time PCR.


� www.biotechdesk.com � T : 040-27017477 / 65916167 F : 040-27016168 � [email protected] 1

� QuickExtract™ DNA Extraction

The QuickExtract™ DNA Extraction Solution permits processing of samplesusing a simple protocol. Aliquots of the QuickExtract Solution are pro-vided as individual sample tubes. To obtain PCR-ready DNA, just rotatethe buccal sample swab in one of these tubes, mix, and heat. No centrifu-gation step is needed; so sample-handling times are very short.

� Catch-All™ Sample Collection Swabs

Soft foam swabs on soft, flexible plastic handles. Catch-All Swabs pro-vide gentle, safe buccal sample collection, even for infants, and theporous foam on these swabs catches more of the sample than buccalbrushes. Catch-All Swabs, also available separately, are provided indi-vidually packaged in sterile hard-pack plastic cylinders. After collectingthe sample, return the sample swab to the cylinder package, for safe,secure storage and transport from the collection site to the analysis site.

• Versatile—recover DNA from the leaves of many plant

species, even those high in polysaccharides and polyphenoliccompounds.

• Rapid—purify DNA in less than one hour.

• Yields high molecular weight DNA.

• Simple and non-toxic—eliminates organic solvent and CTAB

extractions, CsCl gradients, and enzyme digestions.• Recover DNA of higher integrity than column-based

purification methods.

� BuccalAmp™ DNA Extraction Kit

The BuccalAmp™ DNA Extraction Kit is a single-tube system for rapidpreparation of DNA from buccal (cheek) swab samples for use in PCRamplification assays. The kit incorporates the QuickExtract™ DNA Ex-traction Solution, developed by EPICENTRE scientists, that permits pro-cessing of samples using a simplified new protocol.

Benefits

• Simple and rapid sample collection—a swab of the inner cheek

replaces blood draws for obtaining genomic DNA.

• Gentle—the sample is collected using a swab made of soft,

porous foam on a soft, flexible plastic handle.

• Simple and rapid DNA extraction protocol - just rotate the

swab sample in the QuickExtract Solution, mix, and heat.

• Safe—reduces risks from sample collection (e.g., needle sticks)

and uses no phenol, chloroform, or other toxic solvents.

• Amenable to automation and high throughput—no centrifuga-

tion needed.

Cat. No. Product Qty

QEC091H Catch-All™ Sample Collection Swabs 100 swabs

QEC0925 Catch-All™ Sample Collection Swabs 25 swabs

� BuccalAmp™ DNA Extraction Kit

Figure 1. BuccalAmp™ extraction protocol.

� MasterAmp™ Buccal Swab Kits and DNA Extrac-tion Solution

The MasterAmp™ Buccal Swab DNA Extraction Kits are easy-to-use, single-tube systems for the rapid preparation of DNA from human or othermammalian buccal (cheek) cells for PCR amplification. The format allowsprocessing of one to hundreds of samples in less than one hour. Yieldsrange from 0.5-3.0 µg of DNA from a buccal sample, enough to performseveral PCR amplifications. The standard MasterAmp Buccal Swab DNAExtraction Kit contains individually packaged Buccal Swab Brushes in asterile, soft package. The kit for remote site testing contains brushesfor off-site use that are packaged in sterile, hard-pack plastic cylindersfor secure storage and transport from the collection site to the analysissite.

Benefits

• No blood draws are necessary to obtain PCR-ready DNA.

• Simple and rapid extraction protocol.

• Includes MasterAmp PCR Enhancer Technology, which

improves many PCR amplifications.

• Single-tube system assures sample integrity and prevents

contamination.

• Safe—uses no organic solvents.


MB71030 MasterAmp™ Buccal Swab DNAExtraction Kit (standard)* 30 rxns

MB030BR MasterAmp™ Buccal SwabBrushes (standard)** 30

MB79100 MasterAmp™ Buccal Swab DNAExtraction Kit (remote site testing)* 100 rxns

MB100BR MasterAmp™ Buccal Swab Brushes(remote site testing)*** 100

MB7901S MasterAmp™ DNA Extraction Solution 50ml (100 rxns)

Cat. No. Products Qty

BQ0901S BuccalAmp™ DNA Extraction Kits 1 kit (15 rxns)

BQ0908S BuccalAmp™ DNA Extraction Kits 8 kits

BQ0916S BuccalAmp™ DNA Extraction Kits 16 kits

Purify DNA from Buccal Cells/any sampleDNA/ RNA PURIFICATION



QE09050 QuickExtract™ DNA Extraction Solution 1.0 50 ml

*Contents :

MasterAmp™ DNA extraction solution, Swab brushes** Individually packed sterile brushes in soft pack*** Individually packed sterile brushes in hard pack


DNA Purification from Blood, Plant leaf, Soil, Water and FeacesDNA/ RNA PURIFICATION


� MasterPure™ DNA Purification Kit for Blood

• High yield of genomic DNA—up to 10 µg from 325 µl of whole

blood and approximately 100 µg from 600 µl of buffy coat.

• High DNA purity—A260/280

ratios of 1.8-2.0.

• Rapid—can be completed in less than 30 minutes.

• Convenient—the buffy coat protocol uses only 1.5-ml

microcentrifuge tubes and no columns.

• Safe—no organic solvents are used.

• Purified DNA is PCR-ready.

• 5 ml blood samples can be centrifuged to generate 600 µl ofbuffy coat per sample. Each 600ul of buffy coat yield about

100ug DNA on an average.

� MasterPure™ Plant Leaf DNA Purification Kit

• Versatile—recover DNA from the leaves of many plant

species, even those high in polysaccharides and polyphenoliccompounds.

• Rapid—purify DNA in less than one hour.

• Yields high molecular weight DNA.

• Simple and non-toxic—eliminates organic solvent and CTAB

extractions, CsCl gradients, and enzyme digestions.• Recover DNA of higher integrity than column-based

purification methods.

� MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of thereagents needed to purify DNA from gram positive bacteria. These bac-teria lyse more readily after treatment with Ready-Lyse™ and the GramPositive Cell Lysis Solution. Ready-Lyse™ Lysozyme is a stable solution ofa non-mammalian, non-avian recombinant lysozyme, with high specificactivity and no net charge at neutral pH. Thus, there is no waiting todissolve the lysozyme and it does not bind DNA.

Some of the species it has been shown to work on are:

Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Strep-tococcus mutans, Lactococcus lactis


MPP92010 MasterPure™ Plant 10 rxnsLeaf DNA Purification Kit

MPP92100 MasterPure™ Plant Leaf 100 rxnsDNA Purification Kit


MG71100 MasterPure™ DNA Purification Kit 100 rxnsfor Blood


MGP04020 MasterPure™ Gram Positive DNA 20rxnsPurification Kit

MGP04100 MasterPure™ Gram Positive DNA 100rxnsPurification Kit

� SoilMaster™ DNA Extraction Kit

The kit utilizes a hot detergent lysis process combined with a simplechromatography step that removes organic inhibitors, such as humic andfulvic acids, that are known to co-extract with DNA from soil and sedi-ment samples. The SoilMaster™ Kit isolates larger and more intact DNAthan other soil DNA kits incorporating bead beating or vortex mixing.

Benefits• Rapid–extract PCR-ready soil DNA in less than 45 minutes.• Simple–no need for dedicated equipment or bead beating.• Larger, higher molecular weight DNA recovery.• Humic acid and protein removal


SM0250 SoilMaster DNA extraction kit 50 rxns

SC04350 Spin Columns 50 Columns

SR04350 Inhibitor Removal resin 55ml

� WaterMaster™ DNA Purification Kit

The WaterMaster™ DNA Purification Kit provides all of the liquid re-agents needed to purify microbial DNA from any water source afterpassage through a filter of your choice. This capability includes DNAfrom bacteria and eukaryotes.

Benefits

• Simple, gentle procedure – employs no toxic reagents or

mechanical disruption

• Can detect DNA from bacteria as low as 10 per 100 ml

• Small final volume of DNA – 50-fold lesser than competing kits


WM04005 WaterMaster™ DNA Purification Kit 5 rxns

WM04020 WaterMaster™ DNA Purification Kit 20 rxns

� ExtractMaster™ Fecal DNA Extraction Kit

The ExtractMaster™ Fecal DNA Extraction Kit provides all of the reagentsnecessary to recover PCR-ready DNA from the feces of a variety ofanimals. The kit utilizes a detergent lysis process combined with a chro-matography step, which removes enzymatic inhibitors that often co-extract with DNA from such samples. The extracted DNA is PCR-ready,and can be used with the FailSafe™ PCR System to amplify bacterial,protist or animal templates.

Benefits

• Simple, gentle procedure – no toxic reagents or mechanical

disrution• Small final volume of DNA


FD05005 ExtractMaster™ Fecal DNA Extraction Kit 5 rxns

FD05025 ExtractMaster™ Fecal DNA Extraction Kit 25 rxns


Isolate DNA/RNA/mRNA from any sampleDNA/ RNA PURIFICATION


� MasterPure™ Complete DNA andRNA Purification KitsMasterPure™ DNA Purification KitMasterPure™ RNA Purification Kit

MasterPure™ DNA & RNA Purification Kits use a novel technology thatenables efficient purification of intact total nucleic acid (TNA), DNA, orRNA from every type of biological material (Table1).

Targets Samples Extracted

Human & Mammalian SerumRNA & Genomic DNA PlasmaHIV Whole bloodE. coli Guthrie cardsHCV Buccal cellsB. pertussis LiverRSV Mouse tailYeast KidneyM. tuberculosis SalivaEnterovirus UrineHPV SputumSoy (tofu) Tissue culture cell linesMaize Cervical cellsInsect tissues Paraffin-embedded tissuesStreptococcus mutans

Benefits

• Extensive sample range—Purify DNA or RNA from any sample,

every time.

• Quick, easy protocol—purify TNA in 30 minutes, DNA in 45

minutes, and RNA in 60 minutes, without cumbersomecolumns.

• High purity—O.D.260/280

ratios consistently between 1.8 and

2.0.

• High yields e.g. recover >90% of theoretical DNA yield from

E. coli.

• High sensitivity—completely recover even small amounts of

nucleic acid• Safe—no phenol, chloroform, or other caustic solvents.

*Contents:

Red Cell Lysis Solution,Tissue and Cell Lysis Solution, MPC ProteinPrecipitation Reagent, 2X T&C Lysis Solution, TE Buffer, RNase A,

Dnase I, RNase-Free Proteinase K, 1X DNase Buffer.

The DNA Purification kit has only RNAse A and no DnaseIThe RNA Purification kit has only DnaseI and no Rnase.


MC89010 Complete DNA & RNA Purification Kit* 10 rxns

MC85200 Complete DNA & RNA Purification Kit* 200 rxns

MCD85201 MasterPure DNA Purif. Kit 200 rxns

MCR85102 MasterPure RNA Purif. Kit 100 rxns

� mRNA-ONLY™ Prokaryotic mRNA Isolation KitmRNA-ONLY™ Eukaryotic mRNA Isolation Kit

For isolation of rRNA-free prokaryotic/eukaryotic mRNA

The mRNA-ONLY™ Prokaryotic mRNA Isolation Kit provides a novel, simpleand effective method for obtaining mRNA transcripts that aresubstantially free of ribosomal RNA (rRNA) in about an hour. Althougholigo(dT)- or oligo(dU)- immobilized polymers or other substrates arecommonly used to purify polyadenylated eukaryotic mRNA, this has notbeen possible for prokaryotic mRNA since most prokaryotic mRNA is notpolyadenylated. The mRNA-ONLY protocol begins with a purifiedpreparation of total RNA from a prokaryote, such as that obtained usingthe MasterPure™ RNA Purification Kit. The first step of an mRNA-ONLYprotocol is treatment of total RNA with a proprietary mRNA-ONLY™ IsolationSolution, which removes 16S and 23S prokaryotic rRNA in the preparation,leaving a solution that is highly enriched for mRNA. In some protocols,mRNA is then salt-precipitated using the LiCl Solution provided in thekit.


MOP51010 mRNA-ONLY™ Prokaryotic mRNA Isolation Kit 10 purif.

MOP51024 mRNA-ONLY™ Prokaryotic mRNA Isolation Kit 24 purif.

MOE51010 mRNA-ONLY™ Eukaryotic mRNA Isolation Kit 10 purif.

MOE51024 mRNA-ONLY™ Eukaryotic mRNA Isolation Kit 24 purif.

Contents:

Proprietary mRNA-ONLY™ mRNA Isolation Reagents, including mRNA-ONLY™Isolation Solution, RNase Inhibitor, 10X mRNA-ONLY™ Isolation Buffer,Stop Solution, and LiCl Precipitation Solution.

� MasterPure™ Individually Packaged, BulkReagents (10X Size)


MRC0912H Red Cell Lysis Solution 1200 ml

MTC096H Tissue & Cell Lysis Solution 600 ml

MMP095H MPC Protein Precipitation Solution 500 ml

MMP03750 MPC Protein Precipitation Solution 50 ml

MTE0970 TE Buffer 70 ml

MTC085H 2X T&C Lysis Buffer 500 ml

MPRK092 Proteinase K 50 µg/µl 2 ml

D9905K DNase I, RNase-Free 1 U/µl 5 ml

MBD092H 1X DNase Buffer 200 ml

MRNA092 RNase A 5 µg/µl 2 ml

MPS09250 Precipitation Solution for Blood 250 ml

PCR04050 PCR Precipitation Solution 50 x 50 µl Purif.

PCR04250 PCR Precipitation Solution 250 x 50 µl Purif.


Purify DNA/RNA from Yeast, BACs, Fosmids and for MicroarrayDNA/ RNA PURIFICATION


� MasterPure™ Yeast DNA Purification Kit

The MasterPure™ Yeast DNA Purification Kit enables efficient, high-yieldpurification of high molecular weight DNA from yeast and other fungi.The protocol involves non-enzymatic cell lysis at 65°C, followed by re-moval of protein by precipitation, then nucleic acid precipitation andresuspension. No lyticase or proteolytic enzymes are used in the proce-dure.

• Higher yields of yeast chromosomal DNA than with other kits

(Figure 1).

• Versatile—recover DNA from a wide variety of yeast species

including Candida, Saccharomyces, Pichia, andSchizosaccharomyces, and filamentous fungi such asAspergillus2 and Penicillium.

• Rapid—purify DNA in less than 40 minutes.

• Simple and safe—eliminates the need for enzymatic lysis,

columns, and phenol or other organic extractions.

Figure 1. The MasterPure™ Yeast DNA Purification Kit giveshigher yields of DNA than other kits. DNA was quantitated specifi-cally with Hoechst fluorescent dye 33258, which gives minimalfluorescence with RNA.


MPY80010 MasterPure Yeast DNA Purif. kit 10 rxns

MPY80200 MasterPure Yeast DNA Purif. kit 200 rxns

� MasterPure™ Yeast RNA Purification Kit

The MasterPure™ Yeast RNA Purification Kit provides all of thereagents needed to purify RNA from cell types including: Candida,

Saccharomyces, Schizosaccharomyces, and filamentous fungi. The kitutilizes a rapid desalting process to remove contaminating macromol-ecules, avoiding toxic organic solvents, bead-beating, andspheroplasting.

• Avoid hot acid phenol

• Faster than spheroplasting

• Higher quality than bead-beating

• No extra enzyme or equipment to purchase

• RNA can be used for microarray

• Yields 25-50 ug RNA from 1 ml of mid log S. cerevisiae


MPY03010 MasterPure Yeast RNA Purif. kit 10 rxns

MPY03100 MasterPure Yeast RNA Purif. kit 100 rxns

� ArrayPure™ Nano-scale RNA Purification Kit

The ArrayPure™ Nano-scale RNA Purification Kit provides all of thereagents needed to purify RNA from one to a few thousand eukaryoticcells, such as obtained from Laser Capture Microdissection (LCM)procedures.The reagents are all aqueous to avoid the use of toxic organicsolvents.This nano-scale protocol has been developed and tested withquantitative real-time PCR on 1 to 10,000 eukaryotic cells. Cell numberswere based on living cells that were enumerated and then diluted. Theaverage yields of RNA from 1,000 and 10,000 HeLa cells, as determinedby RiboGreen® fluorescence, were 21 and 213 ng, respectively. In addition,µg amounts of RNA have been produced from 20 HeLa cells using aRNA 2-round synthesis techniques.

Applications: RT-PCR, aRNA, LCM


MPS04050 ArrayPure™ Nano-scale RNA 50 Purif.Purification Kit

� BACMAX™ DNA Purification Kit

FosmidMAX™ DNA Purification Kit

The BACMAX™ / FosmidMAX™ DNA Purification Kit was developed foreasy, reliable isolation of high-quality BAC/Fosmid DNA. The scalableprotocol is based on a modified alkaline-lysis procedure that typicallyyields 0.6 to 25 µg of BAC/Fosmid DNA from 1.5 to 100 ml of a single-copyBAC culture. Selective precipitation steps and the incorporation ofEPICENTRE’s RiboShredder™ RNase Blend effectively remove contami-nants that degrade DNA and interfere with downstream applications.

• Higher yields of BAC DNA than with other kits.

• Superior quality— Isolate BAC DNA pure enough to store for

one year at -20°C without being degraded.

• Versatile sample size— Easy to follow protocols for isolating

up to 0.6, 4, 11, or 25 µg of BAC DNA from 1.5, 10, 40, or100-ml cultures of a single-copy BAC, respectively.

• Convenient— Eliminate the need for columns, resins, or

organic extractions.

Reagents sufficient for 150 purifications with 1.5 ml; 30 purificationswith 10 ml; 10 purifications with 40 ml; or 5 purifications with 100 ml.

� PlasmidMAX

For isolation of plasmids, please view page # 16

� GELase

Extraction of DNA from Gels, please see page # 17


BMAX044 BACMAX™ DNA Purification Kit* 1 kit

FMAX046 FosmidMAX™ DNA Purification Kit 1 kit


Whatever the template, whatever its sequence, the FailSafe™ PCR Sys-tem is almost always your best choice for dependable amplification ofany template up to 20 kb every time. If the template is larger than 20 kb,use the MasterAmp™ Extra-Long PCR Kit. For real-time quantitative PCR

applications, use the FailSafe™ Real-Time PCR System.

a 3’-uncoded nucleotide addition (e.g., 3’-terminal A overhang).

b PCR products synthesized using the FailSafe PCR System can becloned with good cloning efficiencies using both blunt-end vectorsand vectors with a 5’-terminal T overhang.

c Mn2+-dependent.

d Note, Taq and Tfl DNA polymerases have been reported to catalyzeMn2+-dependent reverse transcription. However, the level of RTactivity is significantly less than that of RetroAmp™ RT and Tth

DNA Polymerases; therefore we do not recommend Taq or Tfl DNAPolymerase for reverse transcription.

The FailSafe™ PCR System, the MasterAmp™ Extra-Long PCR Kit, and theFailSafe™ Real-Time PCR System require no additional optimizationcomponents. For other thermostable DNA polymerases, EPICENTRE offerstwo different PCR optimization kits. The proper optimization kit shouldbe chosen based upon the polymerase used.

The following table defines the appropriate kit to use for each thermo-stable enzyme.

5→3’

structure

dependent

exonuclease

3’→5’

proofreading

exonuclease

Terminal

transferase

activitya

Reverse

transcriptase

activityc, d

Amplify < 3

kb DNA

Amplify 3-5

kb DNA

Amplify 5-15

kb DNA

Amplify

15-20

kb DNA

Amplify 40

kb DNA

Fail

Safe

™ P

CR

Syst

em

Mast

erA

mp

™Extr

a-

Lon

g P

CR

Kit

Ta

q D

NA

Poly

me

rase

Tfl

DN

APoly

me

rase

Tth

DN

APoly

me

rase

Am

pli

Th

erm

™D

NA

Poly

me

rase

+ + + + + -

+ + - - - -

+b

+ + + + +

- - - - + -

+ - + + + +

+ + + + + +

+ + + + - -

+ + - - - -

- + - - - -

Propertiesof PCR

products

MasterAmp™Taq DNA

Polymerase

MasterAmp™AmpliTherm™

DNA Polymerase

MasterAmp™Tfl DNA

Polymerase

MasterAmp™Tth DNA

Polymerase

MasterAmp™PCR

Optimization Kit

MasterAmp™PCR

OptimizationKit with

Ammonium Sulfate

x

x

x

x

PCROptimization

Kits

PCR PRODUCT SELECTION GUIDEPCR

Polymerase Chain Reaction


� FailSafe™ PCR System

The FailSafe™ PCR System is the only PCR system that ensures successfulPCR, the first time and every time. It provides accuracy, consistency,and dependability for PCR, regardless of the source or the property ofthe DNA templates. The system includes the FailSafe™ PCR Enzyme Mix,an extensively tested set of 12 FailSafe™ PCR “PreMixes”, representingvarious PCR conditions. Along with the FailSafe Enzyme Mix, thesePreMixes contain everything you need for a successful PCR: dNTPs,buffer, and varying amounts of MgCl

2 and FailSafe™ PCR Enhancer (with

betaine).

The FailSafe™ PCR Selection kit has replaced the need for cumbersomeadjustments of reaction components to determine optimal conditions ofPCR of each template and primer pair. The 12 FailSafe™ PCR Premixes inthis kit cover a meticulously determined matrix of PCR conditions thatgive optimal PCR results for different sequences.

Applications

• Amplify any template up to 20 kb• Amplify templates up to 85% GC rich• High sensitivity amplification of as little as 1 copy of

template• High fidelity amplification• Multiplex PCR• No need for hot start

EVERY TIMEGet the selected PreMixwith the FailSafe PCRSystem and use it forconsistent amplificationof your template/primer pair.

FIRST TIMEPerform PCR with yourtemplate and primersusing the FailSafe™ PCRPreMix Selection Kitand choose the PreMixthat provides the bestamplification.

*Contains FailSafe™ PCR Enzyme Mix and the 12 FailSafe™ PCR 2X PreMixes.

Note: Each FailSafe™ PCR System contains FailSafe™ PCR Enzyme Mixand a choice of FailSafe™ PCR 2X PreMixes (any combination; choosebelow):

Cat No. FS99100 includes a choice of one PreMix.

Cat.No. FS99250 includes a choice of two PreMixes.

Cat.No. FS9901K includes a choice of eight PreMixes.

The PreMixes are also available for purchase separately:


FS99060 FailSafe™ PCR PreMix Selection Kit * 1 kit (60U)

FS99100 FailSafe™ PCR System 1 kit (100U)

FS99250 FailSafe™ PCR System 1 kit (250U)

FS9901K FailSafe™ PCR System 1 kit (1000U)

FSE51100 Failsafe Enzyme Mix only 100U

FSE5101K Failsafe Enzyme Mix only 1000U


FSP995A-L FailSafe™ PCR 2X PreMix 2.5 ml

� MasterAmp™ PCR Optimization Kits

MasterAmp™ PCR Optimization Kits are compatible with thermostableenzymes frequently used in PCR, such as Taq, Pfu, Tth, and Tfl DNApolymerases. You can significantly improve the performance of PCR sys-tems in one experiment by adding your own enzyme, template, and PCRprimers into the PreMixes supplied with the kit. The MasterAmp PCROptimization Kits contain a series of 12 MasterAmp™ PCR 2X PreMixesthat have been meticulously tested and represent a complete range ofPCR conditions frequently encountered. Each MasterAmp 2X PreMix con-tains all components needed for PCR except for the primers, the tem-plate, and the PCR enzyme. These components include dNTPs, buffer,and varying amounts of MgCl

2 and the MasterAmp™ PCR Enhancer (with

betaine).

Choice of Optimization KitThere are two types of MasterAmp PCR Optimization Kits available, withand without ammonium sulfate. Choosing the appropriate kit is depen-dent upon the type of thermostable enzyme used in PCR, since ammoniumsulfate is important to the optimal enzymatic activity of certain enzymesand detrimental to that of others.

Cat No. Product Qty.

MOS001 MasterAmp™ PCR Optimization Kit 20 rxns

MO7201 MasterAmp™ PCR Optimization Kit 60 rxns

MOS02N MasterAmp™ PCR Opti. Kit with 20 rxnsAmm. sulfate

MO751N MasterAmp™ PCR Opti. Kit 60 rxnswith Amm. sulfate

MO7205A or MO7205AN → MO7205L or MO7205LN are the cataloguenumbers of the 12 different MasterAmp™ PCR 2X PreMixes to be usedwith the Optimization kit. The product numbers ending in “N” containammonium sulfate.


MO7205A (N)-L (N) MasterAmp™ PCR 2X PreMix 5 ml

� MasterAmp™ Extra-Long PCR Kit

The MasterAmp™ Extra-Long PCR Kit is designed for accuratelyamplifying DNA sequences up to about 40 kb. The MasterAmp™ Extra-Long DNA Polymerase Mix is an enzyme mixture, combining MasterAmp™Taq DNA Polymerase with a 3’→5’ proofreading enzyme, to achieve PCRfidelity at least three times higher than Taq DNA polymerase alone.Included in the kit are MasterAmp Extra-Long DNA Polymerase Mix, a setof 9 pre-optimized Extra-Long PCR 2X PreMixes, and a control templateconsisting of a mixture of lambda DNA and primers. The set of 9 Extra-Long PreMixes represents a complete range of extra-long PCR conditions.Each PreMix contains dNTPs, buffer, and varying amounts of MgCl

2 and

the MasterAmp™ PCR Enhancer (with betaine).


MHF9220 MasterAmp™ Extra-Long 50 rxnsPCR Kit

QU92125 MasterAmp™ Extra-Long 125UPCR DNA Polymerase Mix

QU92500 MasterAmp™ Extra-Long 500UPCR DNA Polymerase Mix

QU9201K MasterAmp™ Extra-Long 1000UPCR DNA Polymerase Mix

MHF925A-I MasterAmp™ Extra-Long 5 ml eachPCR 2X Premixes 1-9

FailSafe™ and MasterAmp™ PCR Optimization KitsPCR

PCR Optimization

MHF925A → MHF925I are the catalogue numbers of the 9 differentMasterAmp™ Extra-Long 2X PreMixes to be used with this kit.


� rBst DNA Polymerase

rBst DNA Polymerase, produced from an overexpressing recombinantclone in E. coli, is the product of the DNA pol I gene of the thermophilicbacterium Bacillus stearothermophilus (Bst). The enzyme has optimalactivity at 65°C and at elevated temperatures it can synthesize DNA inregions containing template secondary structure or high GC contentwhere other non-thermostable DNA polymerases may fail. The enzymehas 5'→3' exonuclease activity. rBst DNA Polymerase is also active as athermostable RNA-dependent DNA polymerase (reverse transcriptase).

� rBst DNA Polymerase, Large Fragment(IsoTherm™ DNA Polymerase)

rBst DNA Polymerase Large Fragment is the product of the DNA pol I geneof the thermophilic bacterium Bacillus stearothermophilus (Bst) alteredto remove the 5'→3' DNA exonuclease activity. Like the Klenow fragmentof E. coli DNA Polymerase I, rBst DNA Polymerase Large Fragment hasstrong strand displacement activity. It also has thermostable reversetranscriptase activity.


BH1100 rBst DNA Polymerase (5 U/µl) 100 U

BH1500 rBst DNA Polymerase (5 U/µl) 500 U

BH101K rBst DNA Polymerase (5 U/µl) 1,000 U

BL901K rBst DNA Polymerase, Large Fragment 1000 U(IsoTherm™ DNA Polymerase) (5 U/µl)

BL1805K rBst DNA Polymerase, Large 5000 UFragment(IsoTherm™ DNA Polymerase) (50 U/µl)

BL1950K rBst DNA Polymerase, 50,000 ULarge Fragment(IsoTherm™ DNA Polymerase) (50 U/µl)

Thermophilic & Mesophilic DNA PolymerasesPCR

Polymerases

Properties of Thermophilic DNA Polymerases

Properties of Mesophilic DNA Polymerases

Product Name

DNA Polymerase I, E. coli

Klenow DNA Polymerase

Exo-Minus Klenow DNAPolymerase

RepliPHI™ Phi29 DNA Polymerase

T4 DNA Polymerase

T7 DNA Polymerase, Unmodified

+ ++ + -

HeatInactivation*

5'→3'exonuclease

3'→5'exonuclease

Nicktranslation

Stranddisplacement

75°C20 minutes

- ++ - +

- - - +

- ++ - ++++

- +++ - -

- +++ - -

75°C20 minutes

75°C20 minutes

65°C10 minutes

75°C20 minutes

75°C20 minutes

Activity

FidelityCReverse

transcriptase3'→5'

exonucleaseStrand

displacement

- +++ - -

+ + - -

Activity

rBst DNAPolymerase LargeFragment,(IsoTherm™ DNAPolymerase)a

MasterAmp™AmpliTherm™ DNAPolymerase

MasterAmp™ TthDNA Polymerase

rBst DNAPolymerasea

MasterAmp™ TflDNA Polymerase

MasterAmp™ TaqDNA Polymerase

5'→3'exonuclease

- - - +++

<15 min at 75°C<1.5 min at 95°C

Thermostabilityb

Product Name

<15 min at 75°C<1.5 min at 95°C

N/A- + - N/A

+ - N/A

- + - N/A

+ - N/A

Very weakRequires Mn2*

++

Requires Mn2*

9 min at 97.5°C

40 min at 95°C

20 min at 95°C

N/A

N/A

N/A

2.2 x 104

0.38-1.82x 104

8.3.9.0 xx 105


� Thermophilic DNA Polymerases (~ 95 °C)

These have optimal DNA synthetic activity above 70°C and can be usedup to 90 °C. These are the following:

� MasterAmp™ AmpliTherm™ DNA Polymerase

It lacks both the 5'→3' structure-dependent exonuclease activity like thatfound in Taq DNA polymerase and the 3'→5' proofreading exonucleaseactivity found in some other DNA polymerases.

� MasterAmp™ Tfl DNA Polymerase

Derived from the thermophilic bacterium Thermus flavus, MasterAmp™Tfl DNA Polymerase is a thermostable DNA polymerase useful for per-forming PCR. EPICENTRE’s MasterAmp Tfl DNA Polymerase has beenshown to produce large PCR products (15 kb).

� MasterAmp™ Tth DNA Polymerase

MasterAmp™ Tth DNA Polymerase is a thermostable DNA polymerase de-rived from the thermophilic bacterium Thermus thermophilus. Theenzyme also has thermostable reverse transcriptase activity, which en-ables synthesis of cDNA from RNA templates. The high reaction tempera-tures possible with MasterAmp Tth DNA Polymerase minimize problemsassociated with nonspecific priming and template secondary structure.

� MasterAmp™ Taq DNA Polymerase

MasterAmp™ Taq DNA Polymerase is a thermostable DNA polymerase de-rived from Thermus aquaticus. The enzyme has an intrinsic 5'→3' struc-ture-dependent exonuclease activity, but lacks 3'→5' proofreading exo-nuclease activity.


AT72100 MasterAmp™ AmpliTherm™ DNA Polymerase (5 U/µl) 100 U



AT7201K MasterAmp™ AmpliTherm™ DNA Polymerase (5 U/µl) 1000 U

AT7205K MasterAmp™ AmpliTherm™ DNA Polymerase (5 U/µl) 5000 U

F72100 MasterAmp™ Tfl DNA Polymerase (1 U/µl) 100 U



F7201K MasterAmp™ Tfl DNA Polymerase (1 U/µl) 1000 U

F7205K MasterAmp™ Tfl DNA Polymerase (1 U/µl) 5000 U

TTH72100 MasterAmp™ Tth DNA Polymerase (5 U/µl) 100 U



TTH7201K MasterAmp™ Tth DNA Polymerase (5 U/µl) 1000 U

TTH7205K MasterAmp™ Tth DNA Polymerase (5 U/µl) 5000 U


Q82100 MasterAmp™ Taq DNA Polymerase (5 U/µl) 100 U



Q8210K MasterAmp™ Taq DNA Polymerase (5 U/µl) 1000 U

Q8250K MasterAmp™ Taq DNA Polymerase (5 U/µl) 5000 U

The buffers and enzyme are available separately also:


FB3715 MasterAmp™ Tfl 20X PCR Buffer 1.5 ml

FB3760 MasterAmp™ Tfl 20X PCR Buffer 5 ml

AT72250N MasterAmp™ AmpliTherm™ DNA Polymerase 250 U(5 U/µl) (enzyme only)

F72250N MasterAmp™ Tfl DNA Polymerase (1 U/µl) 250 U(enzyme only)

TTH7225N MasterAmp™ Tth DNA Polymerase 250 U(enzyme only) (5 U/µl)

Q82250N MasterAmp™ Taq DNA Polymerase (5 U/µl) 250 U(enzyme only)

� Mesophilic DNA Polymerases

DNA Polymerase I, E. coli

DNA Polymerase I from E. coli is a DNA-dependent DNA polymerase. Theenzyme also contains both 5'→3’and 3'→5' exonuclease activities. The5'→3' exonuclease activity enables the enzyme to use nicks and gaps inthe DNA as starting points for labeling the DNA by nick translation.

� Klenow DNA Polymerase

Klenow DNA Polymerase is derived from E. coli DNA polymerase I. Thislarge fragment, DNA-dependent enzyme has 5'→3' polymerization and3'→5' exonuclease activities, but lacks 5'→3' exonuclease activity. KlenowDNA polymerase blunt ends doublestranded DNA with singlestranded over-hangs. The 3'→5' exonuclease activity removes 3' overhangs and the 5'→3'polymerization activity fills in 5' overhangs.

� Klenow DNA Polymerase Fragment Exo-

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase thatlacks both the 5' → 3' and 3' → 5' exonuclease activities of E. coli DNAPolymerase I from which it is derived.


DP02250 DNA Polymerase I, E. coli (10 U/µl) 250 U

DP021K DNA Polymerase I, E. coli (10 U/µl) 1000 U

DP0415K DNA Polymerase I, E. coli (10 U/µl) 5000 U

KP04061K Klenow DNA Polymerase 1000 U

KL04011K Klenow DNA Polymerase Fragment Exo- 1000 U

Thermophilic and Mesophilic DNA PolymerasesPCR

Polymerases


MCQ74200 MasterAmp™ Taq 250PCR Core Kit rxns

Contents:

MasterAmp™ Taq DNA Polymerax,IOX PCR buffer, IOX PCR Enhancer, dNTP Mix 2.5 mm each, 25 mmMgCl

2, Enzyme dilution buffer

Control Template and Primers Mix

� MasterAmp™ Taq PCR Core Kit


� RepliPHI™ Phi29 DNA Polymerase

RepliPHI™ Phi29 DNA Polymerase (f29 DNA Polymerase) is a highlyprocessive enzyme with exceptional strand displacement activity. Theenzyme also contains a 3'→5' exonuclease activity that enablesproofreading capability.

Mesophilic DNA PolymerasesPCR

Polymerases

� T4 DNA Polymerase

T4 DNA Polymerase has both a template-directed 5'→3' DNA polymeraseactivity and a potent 3'→ 5' exonuclease activity. These characteristicsmake the enzyme useful for several molecular biology applications.

Applications

• Conversion of both 5'- and 3'-protruding DNA termini to blunt

ends.

• Cloning of PCR fragments. Treatment of PCR products

containing 3'-A overhangs with T4 DNA Polymerase and dNTPsproduces blunt ends, which greatly increases cloningefficiencies.

• Production of site-specific mutations. Because T4 DNA

Polymerase does not displace oligonucleotides that arehybridized to DNA, it can be used for site-specific mutagenesisby primer extension of “mutated” oligonucleotides hybridizedto single-stranded DNA templates.

• Labeling of 3'-termini of DNA molecules and synthesis of strand-

specific probes using the exonuclease and polymerase activitiesof T4 DNA Polymerase.

� T7 DNA Polymerase, Unmodified

T7 DNA Polymerase has both a template-directed 5'→3' DNA polymeraseactivity and a potent 3'→5' exonuclease activity. The enzyme is a tightly-bound complex of T7 phage-encoded gene 5 protein and E.coli host-encoded thioredoxin. T7 DNA Polymerase is highly processive; itsynthesizes long stretches of DNA before dissociating from the template.The rate of elongation is also much faster than that of most other DNApolymerases.

The unmodified form of T7 DNA Polymerase should not be confused withT7 DNA polymerase preparations from which exonuclease activity hasbeen removed by genetic engineering or by oxidation during purification.

Applications

• Primer extension of long DNA molecules.

• Conversion of 5'- and 3'-protruding ends to blunt ends.

• Labeling of 3'-ends of DNA. In situ detection of DNA fragmen-tation associated with apoptosis.


PP031010 RepliPHI™ Phi29 DNA 10 µgPolymerase (enzyme only) 1 (10,000 U)µg/µl (1000 U/µl))

PP040110 RepliPHI™ Phi29 DNA 10 µgPolymerase (enzyme only) (10,000 U)0.1 µg/µl (100 U/µl)

RH031110 RepliPHI™ Phi29 Reagent Set Enzyme: 10 µg(Enzyme: 1 µg/µl (1000 U/µl)) (10,000 U)

RH040210 RepliPHI™ Phi29 Reagent Set Enzyme: 10 µg(Enzyme: 0.1 µg/µl (100 U/µl)) (10,000 U)

RPB04041 RepliPHI™ Phi29 PolymeraseDilution Buffer 1 ml

� Other Polymerases for PCR

Hot Start Pol is recommended for high specificity PCR reactions andshows superior amplification at lowest template concentrations. Thethermal activation prevents the extension of nonspecifically annealedprimers and primer-dimers formed at low temperatures during PCRsetup. The polymerase contains monoclonal antibodies, which blockpolymerase activity prior to the onset of thermal cycling. It catalyzesthe polymerization of nucleotides into duplex DNA in 5'→3' direction inthe presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonucleaseactivity.

High Fidelity Pol is a blend of DNA polymerases specially designed forhighly accurate and efficient amplification of fragments up to 5 kbpincluding GC-rich or other difficult templates. The enzyme blend includesa highly processive 5'→3' DNA polymerase and shows no detectable 5'→3'exonuclease activity. Its inherent 3'?5' exonuclease proofreading activityresults in a greatly increased fidelity of DNA synthesis compared to Taq.

Long Range Pol is a blend of DNA polymerases specially designed forhighly accurate and efficient amplification of fragments up to 30 kbp.The enzyme blend includes a highly processive 5'→3' DNA polymerase andshows no detectable 5'→3' exonuclease activity. Its inherent 3'→5' exonu-clease proofreading activity results in a greatly increased length of am-plification product compared to Taq.

Sequencing Pol is a Taq polymerase mutant showing an enhanced effi-ciency for incorporation of ddNTPs. Its capability of incorporating ddNTPsand dNTPs at equal rates makes it the ideal choice for DNA cycle sequenc-ing. The enzyme replicates DNA at 74°C. It catalyzes the polymerizationof nucleotides into duplex DNA in 5'→3' direction in the presence ofmagnesium and has a 5'→3' polymerisation-dependent exonuclease re-placement activity only.


D0602H T4 DNA Polymerase 200 U

D0605H T4 DNA Polymerase 500 U

D07250 T7 DNA Polymerase, Unmodified (10 U/µl) 250 U

D07500 T7 DNA Polymerase, Unmodified (10 U/µl) 5000 U

D0701K T7 DNA Polymerase, Unmodified (10 U/µl) 1000 U

* includes enzyme, buffer, dNTPs, DTT


PCR-103L Hot Start Master, L pack 500 rxns

PCR-103S Hot Start Master, S pack, Master mix of 100 rxnsthermally activated DNA polymerasefor high specificity

PCR-203L Hot Start Pol, L pack 1000 U

PCR-203S Hot Start Pol, S pack, Thermally activated 200 UDNA polymerase for high specificity

PCR-104L High Fidelity Master, L pack 250 rxns

PCR-104S High Fidelity Master, S pack, Master 50 rxnsmix of thermostable DNA polymerasefor high accuracy

PCR-204L High Fidelity Pol, L pack 500 U

PCR-204S High Fidelity Pol, S pack, Thermostable 100 UDNA polymerase for high accuracy

PCR-205L Long Range Pol, L pack, Thermostable DNA 500 Upolymerase for amplification of long templates

PCR-202S Sequencing Pol 200 U Taq Pol mutant for incorporation of ddNTPs

PCR-202L Sequencing Pol Taq Pol mutant 1000 Ufor incorporation of ddNTPs


� FailSafe™ GREEN Real-Time PCR System

The FailSafe™ GREEN Real-Time PCR System extends the unsurpassedspecificity, sensitivity, and consistency of the FailSafe™ PCR System toquantitative PCR applications. Like the FailSafe™ PCR System, this kitensures successful quantitative PCR the first time and every time. TheFailSafe™ Real-Time PCR System incorporates SYBR® Green I dye for thedetection of double-stranded DNA generated during PCR. During eachphase of DNA synthesis, the SYBR Green I dye binds to the amplified PCRproducts and the amplicon quantity is monitored in real time by measur-ing its fluorescence. The incorporation of SYBR Green I dye into a real-time PCR reaction provides great flexibility because no target-specificprobes are required.

The system also incorporates an extensively tested set of 12 FailSafe™PCR “PreMixes”, representing various PCR conditions. These PreMixescontain everything you need for a successful quantitative PCR: SYBRGreen I dye, dNTPs, buffer, and varying amounts of MgCl

2 and EPICENTRE’s

patented FailSafe™ PCR Enhancer (with betaine). The system also in-cludes ROX, a passive reference fluorescent dye, which may be requiredfor signal normalization in SYBR® Green dye reactions when using ABIreal-time PCR instruments.

FIRST TIMEPerform real-time PCRwith your template &primers using theFailSafe GREEN Real-time PCR OptimizationKit & select the optimalReal-time PCR Premix.

And EVERY TIMEFor consistent PCRresults, choose the PCRPremix identified asoptimal when you getthe FailSafe GREENReal-time PCR PremixChoiceKit

Benefits

• Real time PCR Premixes contain all components for quantita-

tive PCR using Sybr Green I dye.

• No specific probes required.

• Includes ROX, an internal reference dye.

• Kits available for both tube and capillary instruments.


FSR0360 FailSafe™ Green Real-Time PCR 96- 25 µl rxnsPreMix Selection Kit *

FSR03200 FailSafe™ Green Real-Time PCR 400- 25 µl rxnsPreMix Selection Kit **

FSRC3832 FailSafe™ Green Real-Time 32- 20 µl rxnsCapillary PCR PreMix Selection Kit #

FSRC3896 FailSafe™ Real-Time Capillary 96- 20 µl rxnsPCR System ##

FSRC38384 FailSafe™ Real-Time Capillary 4x96 – 20 µl rxnsPCR System ##

* Contents:

FailSafe™ PCR Enzyme Mix, 12 FailSafe™ Real-Time PCR 2X PreMixesPassive Reference Dye. ** Contents:

FailSafe™ PCR Enzyme Mix. Choice of two FailSafe™ Real-Time PCR 2XPreMixes (Choose any two when ordering; PreMixes are not availableseparately.), Passive Reference Dye.# Contents:

FailSafe™ PCR Enzyme Mix, 8 FailSafe™ Real-Time PCR Capillary 2XPremixes.## Contents:

FailSafe™ PCR Enzyme MixChoice of FailSafe™ Real-Time PCR Capillary 2X Premix(es) (PreMixes are not available separately.)

^Contents:

FailSafe™ PCR Enzyme Blend, 8 FailSafe™ PROBES Real-Time PCR 2XPreMixes, Passive Reference Dye, and Stabilizer.

^^Contents:

FailSafe™ PCR Enzyme Blend, Passive Reference Dye, and stabilizer.FSP51200 includes your choice of any two FailSafe™ PROBES Real-TimePCR 2X PreMixes. FSP5101K includes your choice of any ten FailSafe™PROBES Real-Time PCR 2X PreMixes.

� FailSafe™ PROBES Real-Time PCR System

The FailSafe™ PROBES Real-Time PCR System enables rapid, preciseoptimization of PCR experiments in which the product is detected usingfluorescent target-specific labeled probes. The FailSafe PROBES Real-Time PCR System uses the FailSafe™ PCR Enzyme Blend, which has beenproven to amplify the most difficult templates with extremely high speci-ficity, sensitivity, and fidelity, and a set of 8 specially chosen PCR 2XPreMixes representing a complete range of optimal real-time PCR condi-tions.

Benefits

• Simple, one-step optimization protocol means real-time PCR

reactions that work first time and every time, saving youtime and money.

• Eight PCR Premixes contain all components for screening a

complete range of real-time PCR conditions with yourtemplates, primers and probes.

• Sensitivity, specificity, and PCR efficiency are extremely

high due to the use of a unique enzyme blend and FailSafe™PCR Enhancer technology.

• FailSafe PROBES Real-Time PCR System is compatible with all

real-time PCR instruments and fluorescent probes.

• No hot-start is needed.

• Passive Reference Dye and Stabilizer are included.


FSP51048 FailSafe™ PROBES Real-Time 48 – 25 µl RxnsPCR Optimization Kit^

FSP51200 FailSafe™ PROBES Real-Time 200- 25 µl RxnsPCR PreMix-Choice Kit^^

FSP5101K FailSafe™ PROBES Real-Time 5x200- 25 µl RxnsPCR PreMix-Choice Kit^^

Real Time PCR – SYBR® Green & Probes based systems

PCRReal Time PCR

� TAQurate™ GREEN Real-Time PCR MasterMix

� TAQurate™ PROBES Real-Time PCR MasterMix

It uses a single 2X solution containing everything needed for Real-TimePCR. Just add one volume of a cocktail containing your template, prim-ers, and probes(for the PROBES MasterMix) to one volume of the TAQurateReal-Time PCR 2X MasterMix and run the reaction in your real-time PCRinstrument.


TM049096 TAQurate™ GREEN Real-Time PCR 96 25-µlMasterMix*** rxns

TM046400 TAQurate™ GREEN Real-Time PCR 400 25-µlMasterMix*** rxns

TP411048 TAQurate™ PROBES Real-Time PCR 48 25-µlMasterMix## rxns

TP411200 TAQurate™ PROBES Real-Time PCR 200 25-µlMasterMix## rxns

TP41101K TAQurate™ PROBES Real-Time PCR 1000MasterMix## 25-µl rxns

***Contents:

TAQurate™ Real-Time PCR Enzyme Blend, TAQurate™ GREEN Real-Time2X PCR MasterMix, Passive Reference Dye, and Stabilizer.### Contents:

TAQurate™ PROBES Real-Time PCR 2X MasterMix, TAQurate™ Real-TimePCR Enzyme Blend, Passive Reference Dye, and Stabilizer.


� dNTPs for PCR

High Quality:


D08104 Premixed dNTP solutions 4 ml, 10 µmol(2.5 mM)

D32104 Premixed dNTP solutions 1.6 ml, 10 µmol(6.25 mM)

D59104 Premixed dNTP solutions 400 µl, 10 µmol(25 mM)

D0810A/C/G/T 10 mM dNTP soln. of 1ml of 10 µmoldATP/dCTP/dGTP/dTTP

D03210A/C/G/T 25 mM dNTP soln. of 400 µl of 10 µmoldATP/dCTP/dGTP/dTTP

D05910A/C/G/T 100 mM dNTP soln. of 100 µl of 10 µmoldATP/dCTP/dGTP/dTTP

D1905U 20 mM dUTP soln. 250 µl of5 µmol

For routine applications:


N001 dATP, 100mM water solution 50 µl

N002 dATP, 100mM water solution 250 µl

N003 dCTP, 100mM water solution 50 µl

N004 dCTP, 100mM water solution 250 µl

N005 dGTP, 100mM water solution 50 µl

N006 dGTP, 100mM water solution 250 µl

N007 dTTP, 100mM water solution 50 µl

N008 dTTP, 100mM water solution 250 µl

� Standard DNA Markers


M01 Lambda DNA/HindIII 100 µg

M02 Lambda DNA/HindIII 500 µg

M03 Lambda DNA/Bme18I(AvaII) 100 µg

M04 Lambda DNA/Bme18I(AvaII) 500 µg

M05 Lambda DNA/BssT1I(StyI) 100 µg

M06 Lambda DNA/BssT1I(StyI) 500 µg

M17 Lambda DNA/BglI 100 µg

M18 Lambda DNA/BglI 500 µg

M07 pUC19/MspI 50 µg

� Standard DNA Markers


M11 1 Kb DNA Ladder 50 µg

M12 1 Kb DNA Ladder 250 µg

M15 100 bp DNA Ladder 50 µg

M16 100 bp DNA Ladder 250 µg

M23 100 bp+1.5 Kb DNA Ladder 50 µg

M24 100 bp+1.5 Kb DNA Ladder 250 µg

� Full Range DNA Ladder Bundle


M-201 Full Range DNA Ladder Bundle 500 µl100 lanes each each

� Low range DNA Ladder


M-202 Low range DNA Ladder 100 lanes 500 µl

� Mid range DNA Ladder


M-203 Mid range DNA Ladder 100 lanes 500 µl

� High range DNA Ladder


M-204 High range DNA Ladder 100 lanes 500 µl

dNTPs and MarkersPCR

DNTPs / Ladders

0.5-10 kbp linear scale

The fragment mixture yields a ladder of the following ds fragments:0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6, 8, 10 kbp.Concentration: 100ng/ul

100-300 bp linear scale

The fragment mixture yields a ladder of the following ds fragments:100,150,200,300,400,500,600,800,1000, 1500, 2000, 3000 bp.Concentration: 100ng/ul

50-100 bp linear scaleThe fragment mixture yields a ladder of the following ds fragments:50,75,100,150,200,300,400,500,600,800,1000bp.Concentration: 100ng/ul

The 3 fragment mixtures yield ladders of the following range:50-100bp linear scale: consists of 50,75,100, 150, 200, 300, 400,500, 600, 800, 1000 bp

100-3000 bp linear scale: consists of 100, 150, 200, 300, 400, 500, 600,800, 1000, 1500, 2000, 3000 bp

0.5-10kbp linear scale: consists of 0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6,8, 10kbp.Concentration: 100ng/ul


� Log Scale DNA Ladders

� DNA Ladder Bundle 5

500-5000 bp log scale: 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500,5000 bp200-2000 bp log scale: 200, 400, 600, 800, 1000, 1200, 1400, 1600,1800,2000 bp50-500 bp log scale: 50,100,150,200,250,300,350,400,450,500 bp100-1000 bp log scale: 100, 200, 300, 400, 500, 600, 700, 800, 900,1000 bp1-10 kb log scale: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 kbpConcentration: 100ng/µl


M-211 DNA Ladder Bundle 500 µl00 lanes each each

50-500 bp log scale: 50,100,150,200,250,300,350,400,450,500 bp200-2000 bp log scale: 200, 400, 600, 800, 1000, 1200, 1400, 1600,1800,2000 bp

1-10 kb log scale: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 kbpConcentration: 100ng/µl

� Other Ladders


M-213 50 bp ladder (50-500 bp log scale) 500 µl

M-214 100 bp DNA Ladder (100-1000 bp log scale) 500 µl



M-217 1 kbp DNA Ladder (1-10 kbp log scale 500 µl

� Protein Molecular weight Marker

Mixture of 7 purified proteins supplied in gel loading buffer (50mM Tris-HCl(pH 6.8), 100mM DTT, 2% SDS, 0.1% bromophenol Blue, 10% Glycerol)for direct loading.The proteins are: 116 kDa, 97.4 KDa, 66.2 Kda, 37.6 Kda, 28.5 Kda,18.4 Kda, 14.0 Kda.


PS-101 Protein MW Marker 500 µl

� 10X TA Buffer

TA Buffer is compatible with a wide variety of restriction endonucleasesand nucleic acid modifying enzymes. Use of TA Buffer can reduce thenumber of buffer changes required during nucleic acid sample process-ing, saving time and eliminating sample loss that commonly occurs withbuffer changes. Table 1 shows many enzymes that are active in TA Buffer.

� DNA Preparations


D10 DNA phage lambda (dam-, dcm-) 500 µg

D11 DNA phage lambda 500 µg

D02 DNA phage T7 500 µg

D03 DNA pBR322 50 µg

D04 DNA pBR322 250 µg

D05 DNA pUC19 50 µg

D06 DNA pUC19 250 µg

� MasterAmp™ 10X PCR Enhancer

EPICENTRE’s patented MasterAmp™ 10X PCR Enhancer (with betaine)substantially improves PCR performance and allows improved sensitivityand specificity for amplification of many DNA templates. UsingMasterAmp PCR Enhancer in PCR eliminates the base-pair compositiondependence of DNA melting, increases enzyme’s thermal stability, sup-presses pauses of DNA polymerase, and reduces secondary DNA struc-ture. As a result, long DNA templates; difficult DNA templates (e.g.,those with a high GC content), and those with other complex structurescan be amplified at higher denaturing temperatures, significantly re-ducing the effect of secondary structure without loss of enzyme activity.

� Sterile Nuclease-Free Water

Sterile Nuclease-Free Water is useful for many molecular biology applica-tions. Our water is free of DNA exonuclease and endonuclease activities,and RNase activity.

� Enzyme Storage Buffer

Enzyme Storage Buffer, for dilution of enzymes supplied in this buffer,is liquid at -20°C, but is frozen at -80°C. Most highly-purified enzymesare stable under either of these conditions, but should not be stored atintermediate temperatures between about -30°C to -40°C because thestorage buffer can freeze and thaw, potentially inactivating the enzyme.Some enzymes require storage buffers with cofactors or other compo-nents. Confirm that Enzyme Storage Buffer is suitable for the enzyme.Some enzymes are less stable at very dilute concentrations or you maysee losses in activity due to binding of the enzyme to the walls of thetube.

Enzyme Storage Buffer: 50% glycerol containing 50mM Tris-HCl (pH7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton X-100.


ME81201 MasterAmp™ 10X PCR Enhancer 1.5 ml

ME81205 MasterAmp™ 10X PCR Enhancer 5 ml

ME81210 MasterAmp™ 10X PCR Enhancer 10 ml

FB4250 25 mM MgCl2 25 ml


TA6115 10X TA Buffer 1.5 ml

TA6160 10X TA Buffer 6.0 ml

W7350ML Sterile Nuclease-Free Water 50 ml

ESB4901 Enzyme Storage Buffer 1 ml

Ladders / PCR enhancer / BuffersPCR

Ladders / PCR ENHANCER / Buffers


M-212 DNA Ladder Bundle 500 µl300 lanes each each

� DNA Ladder Bundle 3


� Yield Guarantee

Guaranteed A260

units for unmodified oligos.

Scale (µmole) Desalted Cartridge

0.05 5-10 1.25-2.500.10 8-15 4-50.20 12-20 3-7.51.00 60-80 10-25

Scale (µmole) HPLC PAGE

0.05 1.25-2.50 1.25-2.500.10 4-5 4-50.20 3-7.5 3-7.51.00 10-25 10-25

� Custom Primer / Oligo Modifications

PCRCustom Primer / Oligo Synthesis & Modifications

5’ or 3’ Modification

Phosphate

5’ Carboxyl

3’ Carboxyl

Fluorescein/6-FAM

Primary Amine

TAMRA

5’ Cy5 / Cy3

3’ Cy5 / Cy3

5’ or 3’ Cy5.5 / Cy2

Acridine

Psoralen (C2 / C6)

EDANS/DABCYL

HEX

TET

ROX

JOE

Biotin

Digoxigenin

2’, 3’ Dideoxy A/C/G/T

2, 4 Dinitrophenyl

Backbone modificationsPlease enquire for BackboneModified Oligonucleotides and5’�5’ or 3’�3’ Linkages atdifferent scale of synthesis

Other DNA oligo modifica-tions available.Please enquire.

Cat. No. Sequence (length) Qty

EM-N017 Random mix of all 4 nucleotides ateach position (6) - (dN)

61 OD

260 U

EM-N018 Random mix of all 4 nucleotides 1 OD260

Uat each position (9) - (dN)

9



12



18



24



36


PM-201 T3-Promoter, 17-mer, 10 µM 100 µl (1 nmol)

PM-202 T7-Promoter, 21-mer, 10 µM 100 µl (1 nmol)

PM-203 T7-Terminator, 19-mer, 10 µM 100 µl (1 nmol)

PM-204 SP6-Promoter, 18-mer, 10 µM 100 µl (1 nmol)

PM-205 SP6-Promoter, 24-mer, 10 µM 100 µl (1 nmol)

PM-206 M13/pUC forward, 17-mer (-20), 10 µM 100 µl (1 nmol)

PM-207 M13/pUC reverse, 19-mer (-24), 10 µM 100 µl (1 nmol)

PM-208 M13/pUC forward, 24-mer (-47), 10 µM 100 µl (1 nmol)

PM-209 M13/pUC reverse, 24-mer (-46), 10 µM 100 µl (1 nmol)

� Universal Primers

� Oligo dT


EM-N048 Oligo-dT (10) 1 OD260

U


U


U


U


U


U


U


U


U


U

EM-N058 Mixture of oligo-dT(10) through 1 OD260

Uoligo-dT (100)

� Random Primers

Custom Primer / Oligo Synthesis

� ddNTPs for Sequencing

Please inquire for details

� Universal Primers


P5710F Lambda gt10 Primer, forward (24-mer) 2 µg

P5710R Lambda gt10 Primer, Reverse (24-mer) 2 µg

P5711F Lambda gt11 Primer, forward (24-mer) 2 µg

P5711R Lambda gt11 Primer, Reverse (24-mer) 2 µg

P5706S SP6 Pramoter Primer (24-mer) 2 µg

P5707T T7 Pramoter Primer (24-mer) 2 µg

P5703T T3 Pramoter Primer (23-mer) 2 µg


� MonsterScript™ 1st-Strand cDNA Synthesis Kit

The MonsterScript™ 1st-Strand cDNA Synthesis Kit is optimized for gen-erating full-length first-strand cDNA from multiple mRNA species in samplescontaining total cellular or purified polyadenylated RNA. The cDNA gen-erated is useful for a variety of subsequent applications, including use asa template for PCR, real-time PCR, or other amplification methods.

MonsterScript 1st-Strand cDNA Synthesis Kit uses MonsterScript™ Re-verse Transcriptase, a novel proprietary reverse transcriptase thatlacks RNase H activity. The enzyme’s lack of RNase H activity contributesto its ability to make longer cDNAs and more complete libraries of first-strand cDNA molecules.

Benefits• MonsterScript™ Reverse Transcriptase lacks RNase H, enabling

improved synthesis of full-length cDNA even for long mRNA.• MonsterScript is thermostable, permitting reverse transcrip-

tion at temperatures >50°C, which reduces RNA secondarystructure and improves priming specificity.

• MonsterScript™ RT PreMix contains optimized concentrationsof dNTPS, Mg+2 and Betaine for superior performance andminimal pipetting steps.

• Betaine in the cDNA Synthesis PreMix reduces pausing andstops during reverse transcription.

• The kit includes both an oligo(dT)-containing and a randomnonamer primer.

• First-strand cDNA can be made from picogram amounts oftotal RNA.

• The cDNA is a good template for subsequent end-point PCR orreal-time PCR.

• A potent RNase Inhibitor is included.

� MonsterScript™ Reverse Transcriptase

EPICENTRE’s MonsterScript™ Reverse Transcriptase is a magnesium-de-pendent thermostable reverse transcriptase that completely lacks RNaseH activity. The enzyme is highly efficient at producing full-length cDNAfrom RNA templates up to or greater than 15 kb.

Benefits• Thermostable reverse transcriptase. Full activity up to 65°C

enables reverse transcription through regions of high GCcontent or difficult secondary structure, and increasedspecificity when using gene-specific primers.

• Completely lacks RNase H activity (RNase H-Minus) for full-length cDNA synthesis from RNA up to or greater than 15 kb.

• Greater sensitivity in RT-PCR. MonsterScript ReverseTranscriptase very efficiently produces cDNA from picogramquantities of total RNA. In addition, the MonsterScript ReactionBuffer is compatible with PCR reactions so more of the RTreaction can be used in the PCR reaction.


MS040910 MonsterScript™ 1st-Strand 10 Rxns.cDNA Synthesis Kit

MS041050 MonsterScript™ 1st-Strand 50 Rxns.cDNA Synthesis Kit


MSTA5110 MonsterScript™ Reverse 10 Rxns.Transcriptase

MSTA5124 MonsterScript™ Reverse Transcriptase 24 Rxns.

� MMLV Reverse Transcriptase

MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is anRNA- and DNA-dependent DNA polymerase requiring anoligodeoxyribonucleotide primer for initiation of elongation.It is usefulfor first strand cDNA synthesis in constructing cDNA libraries, as well asfor production of templates for RT-PCR amplifications. MMLV ReverseTranscriptase (MMLV RT) lacks a 3'→5' exonucleolytic proofreading func-tion and has a weak RNase H activity.


M6125H MMLV Reverse Transcriptase (10 U/µl) 2500 U

M6110K MMLV Reverse Transcriptase (10 U/µl) 10000 U

M4425H MMLV Reverse Transcriptase (50 U/µl) 2500 U

M4410K MMLV Reverse Transcriptase (50 U/µl) 10000 U

Includes 10X Reaction Buffer and DTT.

� MasterAmp™ Tth DNA Polymerase

Also has Reverse Transcriptase activity. For details please see page#.

� MasterAmp™ High Fidelity RT-PCR Kit

The MasterAmp™ High Fidelity RT-PCR Kit is ideal for synthesis of accu-rate double-stranded cDNA products that will be used for cloning, se-quencing, expression, or transcription analysis. The kit uses MMLV-RTPlus, a reverse transcriptase with an unsurpassed accuracy and reliabil-ity, and MasterAmp™ TAQurate™ DNA Polymerase, a high fidelity PCRenzyme mix. The kit also contains an optimized 2X RT-PCR Buffer PreMix(containing dNTPs, MgCl

2, and buffer), random and oligo(dT) primers,

and the MasterAmp™ PCR Enhancer with betaine.

Applications

• Highly accurate RT-PCR amplification of RNA templates for• Cloning• Sequencing• Expression• Transcription analysis

• All purpose RT-PCR in which full-length amplification andhigh accuracy are essential.

• Multiplex RT-PCR.

ONE-STEP RT-PCR

The one-step procedure performs first-strand cDNA synthesis and PCRin one tube using RNA specific primers.

STANDARD TWO-STEP RT-PCR

In the two-step procedure, first-strand synthesis is performed usingoligo(dT) or random primers. A small amount of cDNA is then used insubsequent PCR.

cDNA Synthesis and RT-PCRRT-PCR

Reverse Transcriptases


RF91025 MasterAmp™ High Fidelity RT-PCR Kit 25 rxns

RF910100 MasterAmp™ High Fidelity RT-PCR Kit 100 rxns


� MasterAmp™ RT-PCR Kit for High Sensitivity

The MasterAmp™ RT-PCR Kit for High Sensitivity is a single-enzyme,single-tube RT-PCR system. It is capable of amplifying RNA from a singlegene in as little as 1 pg of total cellular placental RNA (e.g., humanchorionic gonadotropin). It is ideal for amplifying low-copy-number RNAtranscripts.

The MasterAmp RT-PCR Kit for High Sensitivity uses RetroAmp™ RT DNAPolymerase, which is both thermostable and has reverse transcriptionactivity. This kit is able to synthesize cDNA at higher temperatures(60ºC). The higher temperature RT can significantly reduce RNA second-ary structure, thus increasing both specificity and sensitivity of RTreactions. The high sensitivity of this kit is further augmented by com-bining both RT and PCR into one single step in which all cDNA synthesizedduring RT is used as template for PCR amplification.

One-Tube One-Step One-Enzyme RT-PCR for Gene Specific PrimersPerform first-strand cDNA synthesis and PCR uninterrupted, in one tubeusing specific primers.

One-Tube Two-Step RT-PCR for Non-Specific PrimersPerform first-strand cDNA synthesis using random oligo dT primers. Inthe subsequent PCR reaction, use cDNA generated and specificprimers.

Benefits

• High sensitivity RT-PCR from as little as 1 pg of total cellularRNA.

• Out-performs similar products from other vendors.• Reduces effect of RNA secondary structure by performing RT

reactions at higher temperature.• Reduces chance for cross contamination by using one enzyme

in a one-step and one-tube reaction protocol with no sampletransfer.

• MasterAmp PCR Enhancer Technology enhances PCR perfor-mance with GC-rich and other difficult templates.


RT71225 MasterAmp™ RT-PCR Kit for High Sensitivity 25 rxns

RT712100 MasterAmp™ RT-PCR Kit for High Sensitivity 100 rxns

Contents:

RetroAmp™ RT DNA Polymerase, 20X RT-PCR Buffer, MasterAmp™ 10XPCR Enhancer, 25 mM MgCl

2, 25 mM MnSO

4, dNTP Mix, 2.5 mM each,

Control Template and Primer Mix, Sterile Water

� Reverse Transcriptase (Sibenzyme)


E317 M-MulV Reverse Transcriptase 1000 U

E318 M-MulV Reverse Transcriptase 5000 U

� MasterAmp™ GREEN Real-Time RT-PCR Kit

The MasterAmp™ GREEN Real-Time RT-PCR Kit provides all the necessarycomponents to perform high-sensitivity one-step quantitative RT-PCRusing SYBR® Green I Dye for detection. The kit uses RetroAmp™ RT DNAPolymerase, a thermostable enzyme that has efficient reverse tran-scriptase (RT) activity up to 70°C and lacks RNase H activity. High-temperature reverse transcription reduces the secondary structure ofRNA templates and results in significantly increased specificity and sen-sitivity.

• Extremely high sensitivity and specificity.

• Easy-to-use PreMix contains all components for successful

quantitative RT-PCR, including SYBR® Green I dye.

• A thermostable enzyme increases sensitivity and specificity

by reducing RNA secondary structure.

• MasterAmp PCR Enhancer increases sensitivity and

specificity by reducing polymerase pausing and stops duringreverse transcription and PCR.

• SYBR® Green I dye for detection saves time and expense

compared to use of labeled primers.• The kit uses flexible protocols for real-time detection on

almost all real-time PCR thermocyclers.

Contents:

RetroAmp™ RT DNA Polymerase, 2X Green RT-PCR PreMix, MasterAmp™10X PCR Enhancer, 25 mM MgCl

2, 25 mM MnSO

4, Sterile Water

� HIV Reverse Transcriptase (Jena Bioscience)

HIV-1 Reverse Transcriptase (RT) is a heterodimer consisting of phage-encoded p66 and p51 subunits. HIV-1 RT is used by the Human Immunode-ficiency Virus-1 to transcribe its RNA genome into single-stranded DNA.HIV-1 RT can be used for incorporation of nucleotide analogs into DNA byRTPCR or as a standard for assaying HIV-1 RT activity in human serum.

Although the p66 subunit is responsible for most of the polymerase activ-ity as well as RNase H activity, the 1:1 complex with p51 subunit showsincreased processivity and activity. The p66 subunit is responsible forpolymerase activity as well as RNase H activity and shows this activityalso in the absence of the p51 subunit. In the absence of p51, the p66subunit forms homodimers.

HIV-2 reverse transcriptase (RT) is used by the Human Immunodefi-ciency Virus-1 to transcribe its RNA genome into single-stranded DNA.HIV-2 RT can be used for incorporation of nucleotide analogs into DNA byRT-PCR or as a standard for assaying HIV-2 RT activity in human serum.In contrast to HIV-1 RT, HIV-2 RT is not inhibited by nonnucleoside RTinhibitors (NNRTI´s).


PR-351 HIV-1 RT(Human Immunodeficiency 10 µgVirus1 Reverse Transcriptase)Recombinant, E. coli

PR-353 HIV-1 RT, p51 subunit (Human 10 µgImmunodeficiency Virus1 ReverseTranscriptase) Recombinant, E. coli

PR-354 HIV-1 RT, p66 subunit(Human 10 µgImmunodeficiency Virus1Reverse Transcriptase) Recombinant, E. coli

cDNA Synthesis and RT-PCRRT-PCR

Kits for RT-PCR/Realtime RT-PCR


MAR03825 MasterAmp™ GREEN Real Time RT-PCR Kit 25 rxns

MAR03100 MasterAmp™ GREEN Real Time RT-PCR Kit 100 rxns


� CopyControl™ cDNA, Gene & PCR Cloning Kits

Up to ~25% more cDNA clones can be obtained in a library if the cDNAclones are present in the host cell at only about one copy per cell (per-sonal communication from P. Carninci and W. Szybalski labs), presum-ably due to reduced detrimental effects on host cell metabolism andlower toxicity of expressed clone sequences and gene products.CopyControl™ cDNA, Gene & PCR Cloning Kits provide a rapid and im-proved method (Figure 1) for cloning any blunt-end DNA fragments, suchas blunt-end genes, PCR products, or cDNAs, at single-copy, with thecapability to induce to high-copies at will. The CopyControl™ pCC1™(Blunt) Vector provided in the kits contains both the E. coli F-factorsingle-copy origin of replication (ori) and an inducible high-copy oriV.Replication does not occur using the oriV unless and until the trfA gene inthe chromosome of TransforMax™ EPI300™ cells, which is under the con-trol of a tightly-regulated promoter, is induced. Thus, CopyControl cDNA,PCR, or gene clones can be grown at single copy to ensure insert stabilityand successful cloning of sequences that are toxic to the host cell. Then,any desired clone can be induced at will to up to 200 copies per cell forhigh yields of DNA for all downstream applications.

Figure 1. Efficient, blunt-end cloning of any cDNA, PCR productor other dsDNA fragment up to 15 kb and screening of clones canbe completed in 24 hours using the CopyControl™ cDNA, Gene &PCR Cloning Kit.

Clones can be kept at one copy per cell for clone stability and then,

whenever desired, any clone can be induced to high-copy-number

(up to 200 copies per cell) for high yields of DNA.

Table 1. PCR Product Cloning Results

Benefits• Get more cDNA or PCR clones and more complete clone

libraries up to 15 kb, including those that may beunstable or encode proteins that are toxic to the E. coli hostcells (Table 1).

• Clones having mutations in genes that are toxic to the hostcell are selectively favored at high-copy.

• Keep clones or a clone library at one copy per cell for insertstability. Then, induce up to 200 copies per cell at will

• Screen the size of the clones in 1 hour or less without theneed for overnight cultures or restriction endonucleasedigests.

Size of the PCR Product cfu/µg of PCR Percent Full-LengthCloned Product Clones

1.4 kb >1 X 107 >90%5 kb 1 X 107 90%10 kb 3.5 X 106 80%15 kb 9.2 X 105 30%


CCECPCR1 CopyControl™ cDNA, Gene & PCR 20Cloning Kit * (with RxnsElectrocompetent cells)

CCPCR1CC CopyControl™ cDNA, Gene & PCR 20Cloning Kit * (with Chemically Rxnscompetent cells)

CCIS125 CopyControl™ Induction Solution 25 ml

*Contents:

CopyControl™ pCC1™ (Blunt-Cloning Ready) Vector, PCR PrecipitationSolution, 10X Reaction Buffer, End-Repair Enzyme Mix, Fast-Link™ DNALigase, EpiLyse™ Solution, EpiBlue™ Solution, TransforMax™ EPI300 Com-petent E. coli, CopyControl™ Induction Solution, Control PCR Product,Supercoiled DNA Size Marker, Water

� PlasmidMAX™ DNA Isolation Kit

PlasmidMAX™ DNA Isolation Kit is designed to purify plasmids free fromRNA and genomic DNA. The resulting DNA is high quality, permittingaverage sequence reads of greater than 600 bases for plasmid templatesbetween 3 and 40 kb. This simple procedure requires only two 1.5-mltubes, thus avoiding excess plastic waste. The purification method isscalable to larger volumes, so separate kits are not needed for varyingsample sizes.

� Plasmid-Safe™ ATP-Dependent DNase

Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminatingbacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clonesor vector preparations. Such preparations are frequently contaminatedwith fragments of bacterial genomic DNA generated during alkaline lysis.Other purification options, such as spin-columns or even CsCl centrifu-gation, do not effectively remove these contaminants and require fur-ther purification steps. Contaminating DNA fragments left behind bythese methods ultimately can become ligated into your cloning vector,resulting in false positives and high backgrounds, or erroneous sequencedata.

Plasmid-Safe ATP-Dependent DNase digests linear double-stranded DNAto deoxynucleotides at slightly alkaline pH and, with lower efficiency,closed-circular and linear single-stranded DNA. The enzyme has no activ-ity on nicked or closed-circular double-stranded DNA or supercoiledDNA.


E3101K Plasmid-Safe™ ATP-Dependent 1000 UDnase** (10 U/µl)



R109AT ATP Solution (500 µl of a 10 mM 5 µmolessolution (pH 7.0))

**Includes Plasmid-Safe™ 10X Reaction Buffer and 25 mM ATP Solution.


PMX51050 PlasmidMAX™ DNA Isolation Kit 50Minipreps

cDNA, Gene, and PCR CloningCLONINGPCR Cloning


� End-It™ DNA End-Repair Kit

The End-It™ DNA End-Repair Kit is used to convert DNA containing damagedor incompatible 5'- and/or 3'-protruding ends that result from shearingor restriction endonuclease digestion to 5'-phosphorylated, blunt-endedDNA for fast and efficient blunt-end cloning into plasmid, cosmid, fosmid,BAC, or other vectors. The conversion to blunt-ended DNA is accomplishedby exploiting the 5'→3' polymerase and 3'→5' exonuclease activities of T4DNA Polymerase. T4 Polynucleotide Kinase and ATP are also included inthe kit for phosphorylation of the 5'-ends of the blunt-ended DNA forsubsequent ligation into a cloning vector.

Benefits• The repaired DNA is blunt-ended and 5'-phosphorylated for

immediate blunt-end ligation into plasmid, cosmid, fosmid, orBAC vectors.

• High specific activity T4 DNA Polymerase and T4 Polynucle-otide Kinase are included for complete conversion of 5'- or 3'-overhang DNA to 5'-phosphorylated, blunt-ended DNA.


ER0720 End-It™ DNA End-Repair Kit 20 rxns

Contents:

End-Repair Enzyme Mix including T4 DNA Polymerase and T4 Polynucle-otide Kinase, End-Repair 10X Buffer, dNTP Solution, ATP

� GELase™ Agarose Gel-Digesting Preparation

GELase™ Agarose Gel-Digesting Preparation contains a unique β-agarosedigesting enzyme developed at EPICENTRE for simple, quantitative re-covery of intact DNA and RNA from low melting point (LMP) agarose gelsfollowing electrophoresis in TAE, TBE, MOPS, or phosphate buffers. Thegel may be digested directly in the TAE, TBE, MOPS, and phosphateelectrophoresis buffers or GELase™ Buffer may be added to or ex-changed with those buffers for higher activity.

Benefits• Gentle procedure—purify even multi-megabase DNA or RNA

that is intact and biologically active.

• Simple, flexible protocols—minimal hands-on time. Equilibra-

tion of the gel slice in GELase Buffer is not necessary, butcan be used for higher activity or greater speed

• Fast—a typical 200 mg gel slice in TAE buffer can be digested

inless than 10 minutes using only 3 units of GELase Preparation• Cost effective—GELase is priced well below spin column or

other gel-digesting methods.


G09050 GELase™ (1 U/µl*) 50 U

G09100 GELase™ (1 U/µl*) 100 U

G09200 GELase™ (1 U/µl*) 200 U

G31050 GELase™ (0.2 U/µl*) 50 U

G31100 GELase™ (0.2 U/µl*) 100 U

G31200 GELase™ (0.2 U/µl*) 200 U

G191ML GELase™ 50X Reaction Buffer 1 ml

G195ML GELase™ 50X Reaction Buffer 5 ml

G1005ML Ammonium Acetate Solution 5 M 5 ml

G1025ML Ammonium Acetate Solution 5 M 5 x 5 ml

*Includes GELase™ 50X Reaction Buffer.

Unit Definition: One unit of GELase Preparation digests 600 mg (~600 µl) ofmolten 1% LMP-agarose gel in GELase Buffer in 1 hour at 45°C.

� DNA Fragment 2X Precipitation Solution

The DNA Fragment 2X Precipitation Solution enables rapid purificationof double-stranded DNA fragments = 200 bp, including, for example, PCRreaction products, DNA fragments obtained in restriction enzyme di-gests, or double-stranded cDNA. If the precipitated DNA fragments are5’-phosphorylated and have ends that are compatible with the vector,they can be used for cloning without further purification

� Transformation and Storage Solution (TSS)

Transformation and Storage Solution (TSS), developed at the U.S. Na-tional Institutes of Health, enables researchers to prepare competent E.

coli in a single step and to transform the cells without heat-shock. TSShas been reported to be faster and easier than other methods of pro-ducing competent cells, such as the traditional CaCl

2 method described

by Sambrook et al. or other high competency protocols. Transformationefficiencies depend on the strain of E. coli, as well as the nature andquality of the transforming DNA. Transformation efficiencies of 106-108

transformants/µg DNA are typical. For example, the transformation ef-ficiencies observed for E. coli strains DH1, DH5a, HB101, JM109, LE392,MM294, SCS-1, and XL1-Blue ranged from 1.5 x 107 to 1.0 x 108 per µg ofDNA.

• Single-step preparation of competent cells.

• Store prepared cells at –70°C with little or no loss in

transformation efficiency.

• Transform cells without heat-shock.

• Transformation efficiencies of 106-108 transformants/µg DNA

are typically obtained

� TypeOne™ Restriction Inhibitor

DNA transformation can be difficult to achieve in many bacterial strainsdue to the presence of one or more restriction and modification (R-M)systems that cleave unmodified DNA that is recognized as “foreign”.TypeOne™ Restriction Inhibitor provides a powerful method for increas-ing transformation efficiencies in bacterial strains with type I R-M sys-tems. Developed as a unique preparation of a phage protein called“ocr”,TypeOne Inhibitor is readily electroporated into cells along withtransforming DNA. In vivo the protein acts as a molecular decoy thatblocks the DNA binding site of type I R-M enzymes and inhibits DNAcleavage.


TY0261H TypeOne™ Restriction Inhibitor 100 µg


C905ML Transformation & Storage Solution 5 ml(TSS)

Cat. No. Product Qty.

DFP51002 DNA Fragment 2X Precipitation Solution 2.5 ml

DFP51012 DNA Fragment 2X Precipitation Solution 12.5 ml

cDNA, Gene, and PCR CloningCLONINGPCR Cloning

� Competent Cells

Please view page # 23


� CopyControl™ Fosmid Library Production KitCopyControl™ HTP Fosmid Library Production Kit

The CopyControl™ Fosmid Library Production Kits provide an efficientand improved method for constructing a library of cosmid-sized (ap-proximately 40 kb) clones. The CopyControl Fosmid Library ProductionKit contains the original pCC1FOS™ Vector, while the recently introducedCopyControl HTP Fosmid Library Production Kit includes a modifiedpCC1FOS Vector, called pCC2FOS (Figure 1). Both of these CopyControlVectors contain both the E. coli F-factor single-copy origin of replica-tion and the inducible high-copy oriV. CopyControl Fosmid clones aregrown at single copy to ensure insert stability and successful cloning ofencoded and expressed toxic protein. The CopyControl Fosmid clonescan then be induced up to 50 copies per cell.

The pCC2FOS Vector contains a primer cassette that optimizes end-sequencing results, especially in a high-throughput setting. This primercassette, engineered in conjunction with Agencourt Bioscience Corpora-tion, eliminates wasteful vector-derived sequencing reads by having the3' terminus of the forward and reverse primers anneal 3 nucleotidesfrom the cloning site. In addition, the 7-base sequence at the 3'-end ofeach primer was specifically designed to minimize mispriming to anycontaminating E. coli DNA present after template purification (Figure2).

TransforMax™ EPI300™ E. coli, required for induction of the CopyControlFosmid clones up to 50 copies per cell, are included with the CopyControlFosmid Library Production Kits.

Figure 1. CopyControl™ Vector map. The CopyControl™ pCC1FOS™and pCC2FOS™ Vectors for CopyControl Fosmid library production aresupplied linearized at the Eco72 I (blunt) site and then dephosphorylatedand is ready for cloning end-repaired (blunt end) genomic DNA of ap-proximately 40 kb.

Figure 2. The CopyControl™ pCC2FOS™ Vector differs from thepCC1FOS™ Vector by the insertion of a new primer cassette thateliminates wasteful vector-derived sequencing reads and minimizesthe potential for priming on the E. coli genome.

Figure 3. Overview of the process for preparing a fosmid libraryusing CopyControl™ Fosmid Library Production Kits. Once the li-brary has been prepared, individual clones can be cultured in smallvolume and induced to multiple-copy number for high yields of highpurity DNA for fingerprinting, sequencing, etc.

Benefits• Make complete and unbiased CopyControl Fosmid libraries.• CopyControl pCC1FOS and pCC2FOS Vectors are supplied

Cloning-Ready—linearized, dephosphorylated, purified, andready for ligation.

• Maximize high-throughput end-sequence results using thepCC2FOS Vector.

• CopyControl Fosmid clones can be induced from single copyup to 50 copies per cell.

• Fosmid cloning utilizes high efficiency lambda packaging thateliminates background and false positives.

• No need for partial restriction endonuclease digests orpulse-field gel electrophoresis (PFGE) to prepare thegenomic DNA for cloning.

Kit is sufficient for producing up to 10 complete and unbiased fosmidlibraries.

*Contents :

CopyControl™ pCC1FOS™ Vector, End-Repair Enzyme Mixl, End-Repair10X Buffer, dNTP Mix, Fast-Link™ DNA LigaseFast-Link™ 10X Ligation Buffer, ATP Solution, GELase™ Gel-DigestingPreparation, GELase™ 50X Reaction Buffer, MaxPlax™ LambdaPackaging Extracts, Control DNA, Ligated Lambda Control DNA,EPI300™ Plating Strain, Control Lambda Plating StrainCopyControl™ Induction Solution

** Same as kit contents above except that it contains CopyControl™pCC2FOS™ Vector instead of pCC1FOS.


CCFOS110 CopyControl™ Fosmid Library Production Kit* 1 Kit

F5FP010 pCC1FOS/pEpiFOS Forward End-SequencingPrimer (50 mM) 1 nmole

F5RP011 pCC1FOS/pEpiFOS ReverseEnd-Sequencing Primer (50 mM) 1 nmole


CCFOS059 CopyControl™ HTP Fosmid LibraryProduction Kit** 1 Kit

HTFP061 pCC2 Forward Sequencing Primer (50 µM) 1 nmole

HTRP062 pCC2 Reverse Sequencing Primer (50 µM) 1 nmole

GENOMIC CLONING – Fosmid Library ProductionCLONING

CopyControl Cloning Systems


� EpiFOS™ Fosmid Library Production Kit

The EpiFOS™ Fosmid Library Production Kit provides all reagents neededto construct up to 10 complete and unbiased primary fosmid librariesusing a novel cloning strategy (Figure 1).

Genomic DNA is first sheared by passing it through a syringe needle. Thesheared DNA is end-repaired to generate 5’-phosphorylated blunt endsand size-selected using a low melting point agarose gel. Finally, the size-selected DNA is ligated into the supplied linearized and dephosphory-lated pEpiFOS™-5 Fosmid Vector (Figure 2), packaged using ultra-highefficiency MaxPlax™ Lambda Packaging Extracts (>109 pfu/µg for phagelambda), and plated on phage T1-resistant EPI100™-T1R E. coli platingcells, all included in the kit. Fosmid pEpiFOS-5 has an F-factor origin, forreplication in E. coli at single-copy and cos sites that enable packaging ofcosmid-sized clones (35-45 kb) in E. coli phage lambda packaging ex-tracts. The end result is a complete, unbiased and stable primary fosmidlibrary.

Note: Fosmid clones constructed in the pEpiFOS-5 Vector cannot beinduced to high-copy number.

Fosmid and BAC Library ProductionCLONING

CopyControl Cloning Systems

Figure 2. EpiFOS™-5 Fosmid Vector.


FOS0901 EpiFOS™ Fosmid Library Production Kit* 1 Kit

F5FP010 pEpiFOS™-5 Forward Sequencing Primer 1 nmole

F5RP011 pEpiFOS™-5 Reverse Sequencing Primer 1 nmole

1 kit is sufficient for producing up to 10 complete and unbiased fosmidlibraries.

*Contents :

pEpiFOS™-5 Fosmid Vector*, End-Repair Enzyme Mix, End-Repair 10XBuffer, dNTP Mix, Fast-Link™ DNA Ligase, Fast-Link™ 10X, LigationBuffer, ATP Solution, GELase™ Gel-Digesting Preparation, GELase™ 50XReaction Buffer, MaxPlax™ Lambda Packaging Extracts, LigatedLambda Control DNA, Control DNA, EPI100™ Plating Strain, ControlLambda Plating Strain

Figure 1. Production of a fosmid library using the EpiFOS™Fosmid Library Production Kit.

� CopyControl™ BAC Cloning Kits(BamH I,EcoR I,Hind III)

CopyControl™ pCC1BAC™ Cloning-Ready Vectors

CopyControl™ BAC Cloning Kits are complete kits containing all molecularbiology reagents you need for constructing high-quality, large-insertBAC (Bacterial Artificial Chromosome) libraries. Each kit has enoughreagents to make a complete human genome-size library at 10X cover-age. The kits feature the CopyControl™ pCC1BAC™ Vector which containsthe E. coli F-factor single-copy origin of replication and an induciblehigh-copy oriV origin of replication. CopyControl BAC clones are grownat single-copy number to ensure insert stability. Then, clones can beinduced to 10-20 copies per cell within 2 hours of adding CopyControlInduction Solution to the culture, for higher yields and higher purityDNA. CopyControl BAC Cloning Kits reduce the time and labor requiredto construct a BAC library.

TransforMax™ EPI300™ Electrocompetent E. coli, or Phage T1-resistantTransforMax™ EPI300™-T1R Electrocompetent E. coli, required for in-duction of CopyControl BAC clones to 10-20 copies per cell, are availableseparately.

The CopyControl pCC1BAC Vector is also sold separately. The vector hasbeen linearized at a unique restriction enzyme recognition site (BamH I,Hind III, or EcoR I), dephosporylated, and highly purified to ensure verylow background.

Fig. 1. The CopyControl™ pCC1BAC™ Vectors are suppliedlinearized at either the unique BamH I-, Hind III-, or EcoR I-site,dephosphorylated. pCC1BAC is derived from pBeloBAC11 andpIndigoBAC-5.


CCBAC1B CopyControl™ BAC Cloning Kit (BamH I) 1 Kit

CCBAC1E CopyControl™ BAC Cloning Kit (Eco RI) 1 Kit

CCBAC1H CopyControl™ BAC Cloning Kit (Hind III) 1 Kit


CBAC311B CopyControl™ pCC1BAC™ 375 ng(BamH I Cloning-Ready) Vector

CBAC311E CopyControl™ pCC1BAC™ 375 ng(EcoR I Cloning-Ready) Vector

CBAC311H CopyControl™ pCC1BAC™ 375 ng(Hind III Cloning-Ready) Vector

BFP0701 pCC1BAC/pIndigoBAC-5 1 nmoleForward End-Sequencing Primer

BRP0801 pCC1BAC/pIndigoBAC-5 1 nmoleReverse End-Sequencing Primer

Kits sufficient for constructing the equivalent of one 10X human BAC lib.


� pIndigoBAC-5 (BamH I Cloning-Ready) VectorpIndigoBAC-5 (Hind III Cloning-Ready) Vector

pIndigoBAC-5 (Figure 1) is derived from pBeloBAC11 and pIndigoBACand will accommodate and stably maintain DNA inserts of >100 kb.pIndigoBAC-5 has been linearized at its unique BamH I or Hind III site,dephosphorylated, and highly purified and is ready for cloning BamH I-or Hind III-cut genomic DNA. The linearized and dephosphorylated vec-tors are tested to ensure the completeness of linearization, dephospho-rylation, and the integrity of the BamH I and Hind III ends.

Once DNA has been ligated into the pIndigoBAC-5 vector, BAC librariescan be produced using the high efficiency TransforMax™ EC100™Electrocompetent E. coli or the Phage T1-resistant TransforMax™ EC100™-T1R Electrocompetent E. coli. These cells accommodate and stably main-tain large BAC clones. Sequencing primers for BAC end-sequencing ofpIndigoBAC-5 clones are available separately.

Note: pIndigoBAC-5 clones cannot be induced to high-copy number.

Figure 1. Cloning-Ready pIndigoBAC-5 Vectors are supplied linear-ized at either the unique BamH I or Hind III site, completely de-phosphorylated, and highly purified.


BACB085H plndigoBAC-5 (BamH I-Cloning Ready) 500 ng

BACH095H plndigoBAC-5 (Hind III-Cloning Ready) 500 ng

BFP0701 plndigoBAC-5 Forward Sequencing Primer 1 nmole

BRP0801 plndigoBAC-5 Reverse Sequencing Primer 1 nmole

Vector are supplied at 25 ng/µl; vector is linearized at BamH I or Eco RIsite and dephosphorylated.

Primer is supplied at 50 µM in TE Buffer.

� Converting Single-Copy BAC or Fosmid Clones toMulti-Copy-Inducible

� EZ-Tn5™ <oriV/KAN-2> Insertion Kit


� BAC-Tracker™ Supercoiled DNA Ladder

The BAC-Tracker™ Supercoiled DNA Ladder is suitable for estimating thesize of large supercoiled DNAs, such as BAC (Bacterial Artificial Chromo-some) clones, by agarose gel electrophoresis. The improved BAC-TrackerLadder contains 7 (rather than 4) discreet supercoiled DNAs rangingfrom 8 to 165 kb (Figure 1). The additional 28 and 165 kb bands improvesize estimations and the 8 kb band facilitates screening for “empty” ornon-recombinant BAC clones.

Supercoiled DNAs of 8, 28, 38, 55, 95, 120, and 165 kb.

Figure 1. Supercoiled BAC DNA is sized by comparison with theBAC-Tracker ™ Supercoiled DNA Ladder . Colonies from 3 differentBAC clones (Lanes 1-3) were processed using the Colony Fast-ScreenKit (Size Screen) and analyzed by agarose gel electrophoresis. M,Improved BAC-Tracker Supercoiled DNA Ladder.

� BAC/Fosmid Clone Purification

� BACMAX™ DNA Purification Kit


� FosmidMAX™ DNA Purification Kit


BAC Cloning- Vectors, Ladders, Purification of ClonesCLONINGBAC Cloning


Cosmid Cloning / Lambda Packaging ExtractsCLONING

Cosmids & Phage extracts

� pWEB-TNC™ Cosmid Cloning KitpWEB™ Cosmid Cloning Kit

The pWEB::TNC™ and the pWEB™ Cosmid Cloning Kits facilitate rapidand efficient construction of unbiased cosmid libraries. The kits utilizea novel strategy of cloning end-repaired, randomly sheared DNA insteadof the conventional approach of cloning fragments generated by partialrestriction endonuclease digestion. First, genomic DNA is sheared bypassing it through a syringe needle (not supplied with either kit). Thesheared DNA is end-repaired to generate blunt ends and size-selectedusing a low melting point agarose gel. The size-selected DNA is thenligated into the supplied linearized and dephosphorylated pWEB::TNC orpWEB Cosmid Vector, packaged using ultra-high efficiency MaxPlax™Lambda Packaging Extracts (>109 pfu/µg for phage lambda), also in-cluded in the kit, and plated on the supplied EPI100™ E. coli plating cells.The result is a complete and unbiased primary cosmid library.

� pWEB::TNC™ Cosmid Cloning Kit

The pWEB::TNC Cosmid Cloning Kit contains the pWEB::TNC CosmidVector. This vector can be used in all the same ways as the original pWEBvector, but offers additional features that can be used if and whendesired. For example, any of the cosmid clones produced in thepWEB::TNC vector can be used for subsequent generation of a randomunidirectional deletion library from any chosen cosmid clone when usedin the conjunction with the pWEB::TNC Deletion Cosmid TranspositionKit.

� pWEB::TNC™ Deletion Cosmid Transposition Kit

The pWEB::TNC™ Cosmid Cloning Kit and pWEB::TNC™ Deletion CosmidTransposition Kit are used in concert to produce a complete randomdeletion library from any primary cosmid clone.

A complete and unbiased primary cosmid library can be constructedeasily and reproducibly in about two days using the pWEB::TNC CosmidCloning Kit. Once the primary cosmid library is produced, the pWEB::TNCDeletion Cosmid Transposition Kit can be used to generate and producea set of random unidirectional deletion clones. The deletion library isproduced simply by incubating any chosen primary cosmid clone withEZ::TN™ Transposase for 2 hours to overnight at 37°C and then trans-forming competent E. coli.

*1 Kit is sufficient for producing up to 10 complete and unbiasedcosmid libraries.

Contents:

pWEB™ or pWEB::TNC™ Cosmid Vector, End-repair Enzyme Mix, End-repair 10X Buffer, dNTP Mix, Fast-Link™ DNA LigaseFast-Link™ 10X Ligation Buffer, ATP Solution, GELase™ Gel-Digestingpreparation, GELase™ 50X Reaction Buffer, MaxPlax™, LambdaPackaging Extracts, Ligated Lambda Control DNA, 40-Kb T7 ControlDNA, EPI100™ Plating Strain, Control Lambda Plating Strain

**Contents:

EZ::TN™ Transposase, EZ::TN™ 10X Reaction Buffer, EZ::TN™ 10X StopSolution, Unlabeled Sequencing Primer, Control Cosmid with 40-kbInsert, Sterile Water


PC8805 pWEB™ Cosmid Cloning Kit* 1 Kit

WEBC931 pWEB::TNC™ Cosmid Cloning Kit* 1 Kit

WEBC942 pWEB::TNC™ Deletion Cosmid 10 rxnsTransposition Kit**

TNC9401 pWEB::TNC™ Cosmid Vector 10 µg

� MaxPlax™ Lambda Packaging Extracts

MaxPlax™ Lambda Packaging Extracts offer maximum in vitro

packaging efficiencies of cos site-containing methylated orunmethylated DNA. Traditional lambda packaging extracts are derivedfrom two complementary lysogenic E.coli strains, BHB2690 andBHB2688, as described by Hohn.MaxPlax Extracts utilize a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690. Thesonicated extracts of NM759 are combined with extracts of BHB2688to produce MaxPlax Extracts.

Each lot of MaxPlax Extracts is tested and guaranteed to maintain apackaging efficiency of greater than 1 x 109 pfu/µg of Control LambdaDNA for one year from the date of purchase if stored properly. ACertificate of Analysis stating actual packaging efficiency (pfu/µgDNA) for each respective lot of MaxPlax Extracts is provided.

Benefits

• High efficiencies—the unique preparation of MaxPlax

Extracts result in packaging efficiencies of up to 3 x 109

pfu/µg DNA.

• MaxPlax Extracts are devoid of all known restriction

activities.

• Packages highly methylated DNA as efficiently as

unmethylated DNA.

Contents:

Pre-dispensed MaxPlax™ Extracts, Control Lambda DNA, Control HostCells (E. coli)


MP5105 MaxPlax™ Lambda Packaging Extracts 5 ext.




� Epi-Grids™ Colony Grid Templates

Epi-Grids™ Colony Grid Templates are easy-to-use griding templatesthat allow rapid organization of colonies from initial screening plates andfor preparing precise master plates. Epi-Grids™ Colony Grid Templatesare made of flexible clear plastic and have a strip of pressure-sensitiveadhesive that allows the grid sheet to adhere to plastic Petri plates, thusensuring correct alignment of colonies on subsequent secondary plates.

Benefits

• Easy and convenient - Simply pull off the protecting adhesive

covering and attach to the bottom of any 10- centimeterpetri dish.

• Two patterns - Epi-Grids Templates are available with either

24 or 48 squares.

� Electroporation Cuvettes

EPICENTRE’s electroporation cuvettes are manufactured to strictquality standards to ensure consistent pulse delivery and reproducibleresults. Electroporation cuvettes are available in 1 mm and 2 mm gapwidths. Compatible with most electroporation instruments includingEppendorf, Bio-Rad, BTX, Invitrogen and others.


EC01091 Electroporation Cuvettes, 1 mm 1 bag (50 pcs.)

EC01082 Electroporation Cuvettes, 2 mm 1 bag (50 pcs.)


EG04850 Epi-Grids™ Colony Grid Templates - 24 Squares 50 Temp

EG048100 Epi-Grids™ Colony Grid Templates - 24 Squares 100 Temp

EG0485H Epi-Grids™ Colony Grid Templates 48 Squares 50 Temp

EG04810H Epi-Grids™ Colony Grid Templates 48 Squares 100 Temp

� Colony Fast-Screen™ Kit (Size Screen)

The Colony Fast-Screen™ Kit (Size Screen), the original Colony Fast-Screen™ Kit, provides a rapid and sensitive method for screening thesize of cloned DNAs without the need to grow cultures or performminipreps or restriction endonuclease digestions. The size of most clonescan be determined in 1 hour or less. Bacterial Artificial Chromosome(BAC) clones can be estimated in as little as 4 hours.

Benefits

• Rapid—determine the size of PCR, cDNA, and other clones in1 hour and of BAC clones in as little as 4 hours without theneed to grow cultures or perform restriction digests.

• Sensitive—the size of single-copy BAC clones are readilyestimated.

• High-throughput capability—the kit is amenable to both high-throughput and routine cloning applications.

• Flexible—can be used with all standard E. coli host strains.


FS08250 Colony Fast-Screen™ Kit (Size Screen)* 1 Kit

*Reagents are sufficient to green 200 colonies.Contents: EpiLyse™ Solution, EpiBlue™ Solution

� Colony Fast-Screen™ Kit (PCR Screen)

The Colony Fast-Screen™ Kit (PCR Screen) provides a rapid method forpreparing clones for screening by PCR. Using the Colony Fast-Screen Kit(PCR Screen) there is no need to grow cultures or purify DNA prior toPCR. The kit can be used with all standard E. coli hosts and all cloningvectors. Thermostable polymerase and PCR primers are not provided.

Benefits• Rapid—identify desired clones by PCR without the need to

grow cultures or purify DNA.• Sensitive—enables PCR screening of high-copy clones, single-

copy clones, and bacterial genomic DNA.• High-throughput capability—the kit is amenable to both high-

throughput and routine cloning applications.• Flexible—enables both long and short PCR amplifications and

can be used with all standard E. coli host strains.

Reagents are sufficient to screen 200 colonies

**Contents: PCRLyse™ Solution, Gel Loading Solution

� Colony Fast-Screen™ Kit (Restriction Screen)

The Colony Fast-Screen™ Kit (Restriction Screen) provides an easy andrapid method to screen the size and orientation of cloned DNA. There isno need for overnight cultures or plasmid minipreps. The Colony Fast-Screen™ Kit Restricti-Lyse™ solution lyses cells in an environment, whichis not inhibitory to restriction enzyme activity. Recombinant clone-con-taining E. coli colonies can be screened in 25 minutes using the standardprotocol, or in as little as 10 minutes with the accelerated protocol.


FS0472H Colony Fast-Screen™ Kit (Restriction Screen)# 1 Kit


FS0322H Colony Fast-Screen™ Kit (PCR Screen)** 1 Kit

Screening Colonies/ Electroporation CuvettesCLONING

Screening and Electroporation

Other electroporation cuvettes also available.

Pleae view page # 73# Contents : Restricti-Lyse™ Solution & Gel Loading Solution.

Sufficient reagents to screen 200 colonies.


Competent Cells for PCR & Genomic CloningCLONING

Competent Cells

Endonucelease A is a DNase that can survive boiling lysis and can degrade plasmid DNA, reducing theDNA yield from plasmid purifications.

A deletion in the lacZ gene, which codes for part of the amino-terminal of the β-galactosidaseenzyme. A plasmid containing sequences of the amino terminus can complement this deletion to givefunctional β-galactosidase. When X-gal is used as the substrate, β-galactosidase produces a blueproduct; the colonies of these cells appear blue. When the complimentary sequence on the vector isdisrupted by inserting a cloned fragment of DNA, the enzyme is no longer functional and cannotproduce a blue product; the colonies of these cells are white. On a plate of mixed blue and whitecolonies, the white colonies should contain the cloned DNA.

The recA gene codes for a DNA-dependent ATPase that is essential for genetic recombination in E.

coli. Bacterial strains deficient in recA are defective in recombination. Plasmids in these strains aremaintained as monomers (do not form multimeric circles) and are less likely to incur deletions.

Restriction systems are designed to protect bacterial cells from foreign DNA. Deleting the host cells’restriction system allows efficient cloning of DNA with different methylation patterns, such as DNAfound in mammals, higher plants, and many prokaryotes

Endonucleaseminus(endA 1)

lacZ ∆∆∆∆∆ M15

Recombinationminus(recA 1)

Restriction minus(mcrA, ∆∆∆∆∆[mrr-hsdRMS-mcrBC])

All EPICENTRE Competent Cells have the following features :

Electrocompetent Cells

Strain Name

Used withSpecific

Vector Ori

TransformationEfficiency

(cfu/µg pUC19)

CatalogNumber

AcceptsLargeDNA

ApplicationsT1 & T5Phage

Resistant

TransforMax™EC100™

No >1 x 1010 No 145 kb General Purpose

EC100055 x 100 µlEC1001010 x 100

µl

EC0205T15 x 100 µlEC0210T110 x 100

µl

ECP095005 x 100 µl

EC6P095H5 x 100 µl

EC3001055 x 100 µlEC30011010 x 100 µlEC30015050 x 100

µl

EC02T155 x 100 µl

EC02T11010 x 100

µl

C400EL1010 x 50 µl

TransforMax™EC100™-T1R

TransforMax™EC100D™pir+

TransforMax™EC100D™ pir-

116

TransforMax™EC1300™

TransforMax™EPI300™-T1R

CopyCutter™EPI400™

Use with CopyControl™Vectors for inducible

single to multiplecopies/cell

Clone toxic genes andunstable DNA

Controls copy numberof common vectors





Rescue cloning usingEZ-Tn5™ or HyperMu™

Transposome™Complexes

Rescue cloning usingEZ-Tn5™ or HyperMu™

Transposome™Complexes

General Purpose145 kb

Upto 100 kb

Upto 50 kb

At least 145kb Good for

Libraries

At least 145kb Good for

Libraries

145 kbColE1-typereplicons

Vectorscontaining both

F-factor and oriVreplicons

Vectorscontaining both

F-factor and oriVreplicons

R6Kγ ori

250 copies/cell

R6Kγ ori

15 copies/cell

No>1 x 1010

>5 x 109

>5 x 109

>1 x 1010

>1 x 1010

>1 x 1010

Yes

No

No

No

Yes

Yes



EC10005 TransforMax™ EC100™ Electrocompetent E. coli 5 x 100 µl

EC10010 TransforMax™ EC100™ Electrocompetent 100 x 100 µlE. coli

CC02810 TransforMax™ EC100™ Chemically 10 x 50 µlCompetent E. coli

EC0205T1 TransforMax™ EC100™-T1R 5 x 100 µlElectrocompetent E. coli

EC0210T1 TransforMax™ EC100™ 100 x 100 µlElectrocompetent E. coli

CCT10210 TransforMax™ EC100™-T1R 10 x 50 µlChemically Competent E. coli

� CopyCutter™ EPI400™ Electrocompetent E. coliCopyCutter™ EPI400™ Chemically Competent E. coli

CopyCutter™ EPI400™ E.coli* cells were developed to significantly lowerthe copy number of a wide variety of common vectors so that you canmore readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or con-tains AT- and GC-rich sequences or sequences with strong secondarystructure. The CopyCutter EPI400 cell line was derived from our high-transformation efficiency phage T1-resistant TransforMAX™ EC100™ E.coli

strain by manipulating a gene that controls the copy number of vectorscontaining ColE1 or pMB1 origins of replication (e.g., pUC- and pET-typevectors). This constitutively expressed gene, pcnB (plasmid copy num-ber), was deleted from the TransforMAX EC100 strain and replaced witha modified pcnB gene that is linked to an inducible promoter, creatingthe CopyCutter EPI400 strain.

The copy number of ColE1-type vectors in the CopyCutter EPI400 straincompared to the parental TransforMAX EC100 strain is approximately 4-to 25-fold lower, depending on the vector. Moreover, following a shortincubation in the presence of the CopyCutter™ Induction Solution, youcan increase the copy number of the vector to improve plasmid yields.


C400EL10 CopyCutter™ EPI400™ 10 X 50 µlElectrocompetent E.coli

C400CH10 CopyCutter™ EPI400™ Chemically 10 X 50 µlCompetent E.coli

CIS40025 CopyCutter™ Induction Solution (1000x) 25 ml

Includes CopyCutter™ Induction Solution and pUC19 Control DNA.

Competent Cells for PCR & Genomic CloningCLONING

Competent Cells


EC300105 TransforMax™ EPI300™ 5 X 100 µlElectrocompetent E. coli*



C300C105 TransforMax™ EPI300™ 10 X 50 µlChemically Competent E. coli*


EC02T15 TransforMax™ EPI300™-T1R 5 X 100 µlElectrocompetent E. coli**

EC02T110 TransforMax™ EPI300™-T1R 10 X 100 µlElectrocompetent E. coli**

CT1C0210 TransforMax™ EPI300™ T1R 10 X 50 µlChemically Competent E. coli**

*Includes CopyControl™ Induction Solution and pUC19 control DNA.

**Includes pUC19 Control DNA

Chemically Competent Cells

Strain Name

TransforMax™EC100TM™

TransforMax™EC100™-T1R

TransforMax™EPI300™

TransforMax™ECI300™-T1R

CopyCutter™EPI400™

No

Yes

No

Yes

Yes

CC0281010 x 50 µl

>5 x 107

>5 x 108

>5 x 108

>1 x 107

>1 x 108

C300C10510 x 50 µl

CCT1021010 x 50 µl

CT1C021010 x 50 µl

C400CH1010 x 50 µl

Vectors containingboth F-factor and

oriV replicons

Vectors containingboth F-factor and

oriV replicons

ColE1-typereplicons

No

No

Used with SpecificVector Ori

TransformationEfficiency

(cfu/µg pUC19)

CatalogNumber

AcceptsLargeDNA

Applications

At least23 kb

At least23 kb

At least23 kb

At least23 kb

At least23 kb

General Purpose

General Purpose







Controls copy number ofcommon vectors


T1 & T5Phage

Resistant


Mammalian cDNA Libraries and ClonescDNA LIBRARIES AND CLONES

Ready Clones

We offer an extensive library of mammalian cDNA clones, includingcomplete full-length cDNAs, partial full-length cDNAs, ESTs, and ORF-onlyclones.

� Mammalian Gene Collection (MGC)the gold standard collection of full-length cDNAsfor human, mous, and rat

Every MGC clone is fully sequenced and verified to contain a completeopen reading frame (ORF). Full-length cDNAs provide the strongest evi-dence for the structure of protein-encoding mRNA transcripts and areindispensable tools for determining gene function. MGC is the largestsingle collection of confirmed full-length and annotated cDNAs. The MGCis generated by a consortium of leading, NIH-funded laboratories and isavailable to all without restriction.

� Assay-Ready MGC cDNADiscover novel gene functions with genome-scaleoverexpression screening

Assay-Ready MGC cDNA draws on the gold standard Mammalian GeneCollection (MGC) to provide you with over 16,000 verified full-lengthhuman and mouse cDNAs cloned into the mammalian expression vectorpCMV-SPORT6. DNA is spotted in 384-well microplates provided in dupli-cate, allowing you to begin screening without first performing thousandsof plasmid preps. Easy protocols for cDNA overexpression assays areprovided as well as additional cDNA plates for assay optimization.

� BioTrack Human Gene Subsets

These subsets focus on well-known human gene families as well as impor-tant cellular pathways and processes. Each subset includes 3 parallelcomponents: full-length cDNA clones (from the Mammalian Gene Collec-tion and the Incyte Gene Collection), Human ORF clones, and ExpressionArrest™ shRNA clones that enable the use of alternative or complemen-tary strategies for determining gene function. For example,overexpression with full-length cDNA clones can be used to confirm thespecificity of RNAi knockdown in a screen with Expression Arrest shRNA.

Developed by Marc Vidal and coworkers, the BioTrack Human ORF clonescontain ORF-only sequences (full-length clones with UTRs removed) de-rived from the MGC and cloned into a recombinational entry vector.These clones can be used in a wide range of applications including yeasttwo-hybrid screens, protein imaging in live cells, cellular and in vitro

assays, and protein overexpression.and purification.

� Human ORF Collection

The Center for Cancer Systems Biology of the Dana-Farber Institute hascreated a path-breaking collection of ORF-only clones (full-lengthcDNAs with UTRs removed) beginning with over 8000 genes1 and withfuture updates planned. These clones are derived from fully sequencedMammalian Gene Collection (MGC) full-length cDNAs and cloned into arecombinational entry vector.

• ORF clones provide a shortcut to protein expression, allowing

you to skip PCR, cloning into an expression vector, and verify-

ing the ends of the ORF DNA sequence.

• Each CDS was amplified for only 25 cycles with gene-specific

primers and KOD HiFi Polymerase, greatly minimizing the risk

of PCR-induced mutations.

• The Gateway® entry vectors ensure easy transfer into prokary-

otic, mammalian, viral, or insect expression systems. For

maximum flexibility, the ORF stop codon has been removed.

� Human Freedom ORFs

Freedom™ ORF clones are collections of high quality sequence verifiedgenes cloned into vectors that are free from the IP constraintscommonly found with other recombinational cloning systems. Eachclone has been amplified and sequenced to ensure the highest quality

standard.

� BD Creator™ Universal Clones

Open Biosystems offers a set of over 1,100 human ORF clones, the BDCreator™ Universal Clones, which represents the initial release of theFreedom™ ORF collection. The BD Creator™ system is a flexible recombi-national cloning system based on Cre-loxP reactions. These ORF clonesare provided to you in the pDNR-Dual vector, allowing you to move theinserts directly into any expression vector of choice through a simpleCre recombinase reaction. Although Freedom™ ORF clones can besubcloned into any expression vector using traditional restriction en-zyme and ligase cloning methods, the Creator™ System allows the targetgene to be rapidly transferred in-frame to a variety of expression vec-tors. The BD Creator™ Universal Clones are constructed for immediate 3'tagging with a 6xHN affinity tag.

The BD Creator™ System transfers a gene from a specialized Donorvector into any Creator™-compatible expression vector, called an Ac-ceptor Vector. The Cre-LoxP recombination reaction transfers the Free-dom™ ORF directionally to the Acceptor vector without the loss or addi-tion of sequence. The resulting expression clone contains the ORF in bothproper orientation and reading frame. A variety of BD Creator™-com-patible expression vectors are commercially available or you can adaptthe vector of your choice to the BD Creator™ System using a 161-bpcassette.

� Incyte Gene Collection (IGC)cDNAs for human, rat, monkey, and dog

As a complement to the MGC, we offer the largely untapped Incytecollections for human, rat, monkey, and dog. A wide range of tissues arerepresented in each collection and together they contain over one millioncomplete full-length cDNAs, partial full-length cDNAs, and ESTs. “Com-plete full-length” cDNAs have been full-insert sequenced and contain acomplete ORF. “Partial full-length” cDNAs have been full-insert sequenced,but do not contain a complete ORF. While not fully annotated, thesecollections may be mined by BLASTing query sequences against our Incyteclone databases.

� Mammalian IMAGE ESTs

The IMAGE consortium was formed in 1993 to provide a public resourceto aid in gene discovery that would be free of patents/royalties andother restrictions. The IMAGE clone collection provides the community aresource of arrayed cDNA clones from various research efforts. Theclones are from oligo dT-primed, directionally cloned plasmid cDNA li-braries from human, mouse, rat, zebrafish, Xenopus and primate sources.The Cancer Genome Anatomy Project (CGAP) was formed in 1996 toinvestigate genes associated with cancerous cells at the molecular level.As a result, CGAP is responsible for thousands of expressed sequencetags (ESTs), which are continuously added to the IMAGE collection.

� Other ESTs

Human Cystic Fibrosis CollectionHuman Eye ESTsMouse BMAP cDNAMouse NEIBank cDNA ClonesMouse NIA cDNA ClonesMouse Organ of Corti CollectionMouse Retina cDNA ClonesRat Non-Redundant cDNAsPorcine ESTs


Mammalian and Non Mammalian cDNA Libraries and ClonescDNA LIBRARIES AND CLONES

Ready Clones

� Brain cDNA Libraries

Average insert sizes range from 2.1-2.3kb and all four libraries contain100% recombinants. The vector used to construct these libraries is Ex-press 1 and the product shipped contains DH10B in SOC plus ampicillinand 20% glycerol. Tissue for these libraries originated from varioussources:

Human Adult Brain Library - Brain tissue from three male donors aged23-27 years old

Human Fetal Brain Library - Brain tissue from one 24 week old maledonor Mouse Brain Library - Brain tissue from 8 week old male C57Bl/6Jmice

Rat Brain Library - Brain tissue from 8 week old, male Sprague-Dawleyrats

� Incyte cDNA Libraries

This unique resource contains human, mouse, canine, wheat, rice, po-tato, Arabidopsis, corn and primate cDNA libraries. Nearly 400 IncytecDNA libraries are available from a wide range of tissues. Libraries areprovided as plasmids to facilitate transportation and storage. Uponreceipt, libraries should be transformed into ultrapure electrocompetentcells.

� NON MAMMALIAN cDNA

� cDNA from Bacteria

� Brucella ORF Collection

Created by a consortium of laboratories, the Brucella ORF Collectionis comprised of 3091 ORFs that have been cloned into a Gateway® entryvector and are ready to be transferred into Gateway destination vec-tors for protein expression and functional analysis. For example, pro-tein-protein interactions can be studied by transferring the collectionto bait and prey plasmids for yeast two-hybrid screening or to a desti-nation construct that expresses each ORF with an affinity, epitope, orfluorescent tag.

� C. jejuni ORF Collection

Jodi Parrish et al from the Center for Molecular Medicine and Genetics,and Department of Biochemistry and Molecular Biology, Wayne StateUniversity have cloned 1,619 ORFs from Campylobacter jejuni into E.

coli using in vivo recombination.

E. coli SURE clones containing the C. jejuni ORFs in the vector pTLJ03are available. pTLJ03 is an expression vector that generates N-terminalGST-His-tagged fusion proteins from the C. jejuni ORF set.

� cDNA from FISH

� Fugu IMAGE cDNAs

� Stickleback cDNA Collections

The three-spine stickleback (Gasterosteus aculeatus), a teleost fish, isan evolutionary model for morphological change that is growing in popu-larity. (It was mentioned under Science’s “Breakthrough of the Year” for2005.) We offer 6 collections of cDNAs originating from specific tissuesin different geographical populations.

� Zebrafish cDNA Library

The amplified version of the Danio rerio primary library used to createthe arrayed I.M.A.G.E. libraries NIH_ZGC_10 and NIH_ZGC_7 (normal-ized). Inserts were directionally cloned using oligo(dT) primers andwere ligated at the EcoRV and NotI sites.

� Zebrafish Gene Collection (ZGC)

The Zebrafish Gene Collection (ZGC) is a complete set of full-length cDNAclones of expressed genes for zebrafish. Each clone is supplied in aplasmid vector transformed into E. coli.

� Zebrafish IMAGE cDNAs

The IMAGE clone collection provides the community a resource of ar-rayed cDNA clones from various research efforts. The clones are fromoligo dT-primed, directionally cloned plasmid cDNA libraries from hu-man, mouse, rat, zebrafish, Xenopus and primate sources. The CancerGenome Anatomy Project (CGAP) was formed in 1996 to investigategenes associated with cancerous cells at the molecular level. As a result,CGAP is responsible for thousands of expressed sequence tags (ESTs),which are continuously added to the IMAGE collection.

� Zebrafish IMCB Collection

Singapore’s Institute for Molecular and Cell Biology (IMCB) has isolatedover 15,000 unique genetic markers from the zebrafish (Danio rerio). Atotal of 15,590 unique zebrafish EST clusters from two cDNA libraries(primary and normalized) have been identified. This EST set is comple-mentary to the existing ESTs in the public database and will be invaluablein assisting in the annotation of genes based on the upcoming zebrafishgenome sequence.

� Zebrafish Morpholino Collection

The Zebrafish Morpholino Collection contains hundreds of oligos designedand synthesized to rapidly reduce gene expression levels. Each sequenceis designed to block the translational start site of the targeted gene. Asmorpholinos are synthesized synthetically and are nuclease resistant, theexpression level reduction is specific and prolonged. Each gene’s startsite is identified and a morpholino is produced that is complementary to~25 bases -25 to +25 bases from the start.

� Zebrafish NEIBank cDNA Clones

� Drosophila Gene Collection (DGC)

The Berkeley Drosophila Genome Project has now analyzed over 240,000Drosophila ESTs. Over 10,000 non-redundant cDNA clones, covering mostof the predicted 13,601 genes in the Drosophila genome are now availableand comprise the Drosophila Gene Collection (DGC).

� Xenopus IMAGE cDNAs

The clones are from oligo dT-primed, directionally cloned plasmid cDNAlibraries from human, mouse, rat, zebrafish, Xenopus and primatesources. The Cancer Genome Anatomy Project (CGAP) was formed in1996 to investigate genes associated with cancerous cells at the molecularlevel. As a result, CGAP is responsible for thousands of expressed se-quence tags (ESTs), which are continuously added to the IMAGE collec-tion.


� cDNA from C.elegans

� C. elegans ORF Collection

Dr. Marc Vidal’s laboratory at the Dana-Farber Cancer Institute hasgenerated the C. elegans Open Reading Frame (ceORF) clones repre-senting over 10,000 worm genes. The ceORFs were generated by ampli-fication from full length cDNA libraries and cloning into the Gateway2

recombinational cloning system (pDONR201). ceORF clones can besubcloned into any expression vector, using traditional restriction en-zyme and ligase cloning methods. Alternatively, the recombinational clon-ing system allows the target gene to be rapidly transferred in-frame intoa variety of expression vectors. Release 1.1 clones represent a pool ofcloned products, thus multiple splice variants are preserved in eachpool. ceORFs will be available in two different formats: glycerol stocksand spotted onto IsoCode® cards.

� C. elegans ORF-RNAi Collection

The C. elegans ORF-RNAi Collection includes clones containing full openreading frames for over 11,000 genes, ready for your RNAi screens!These clones are derived from the C. elegans ORFeome Library v1.1. The C. elegans ORF-RNAi Library provides you comprehensivecoverage for RNAi screening.

• Full Coding Regions: From Start-to-Stop, the entire open

reading frame is cloned into an RNAi feeding vector andcompatible host.

• Feeding Ready: The cloned ORFs have been transformed

into the RNAi feeding bacterial strain HT115(DE3)2 and thefeeding vector, PL4440.

• RNAi Versatility: Plasmid preps of the ORF-RNAi clones can

be used as templates for in vitro dsRNA synthesis for soakingor injection

� C. elegans Promoter Collection

The C. elegans Promoter Collection from Open Biosystems includes clonescontaining the predicted promoters for over 6,000 genes designed toeasily construct GFP reporter strains. Get ready to localize your proteinexpression patterns on a scale you never thought possible.

� cDNA from Yeast

� Candida albicans Genomic Library

The library is ready for transformation into the amenable Saccharomy-

ces cerevisiae to allow rapid, large-scale screens of C. albicans genesthat can generate a phenotype of interest. The library is supplied as anE. coli culture. The average size of inserts is ~4.3 Kb and they have beencloned into pRS426 (2u, URA3), a S. cerevisiae high copy vector. Thenumber of clones in the library is ~50,000 that covers the haploid genome14-fold and greater than 97% of the clones contain inserts.

� HA-Tagged Yeast Strains

The HA-Tagged Yeast Collection contains over 2,400 yeast mutagenizedstrains each producing a single protein with an inserted triple hemagglu-tinin epitope tag (3xHA). The HA insertion potentially provides severalmeans by which yeast protein function may be studied. Native amountsof protein can easily be purified from individual yeast strains usingimmunoprecipitation with a commercially available HA antibody. Also, HAinsertion may potentially generate conditional alleles and hypomorphicmutants that exhibit partial gene function of particular importance inthe analysis of essential genes.

� Yeast Insertional Mutant Strains

The Yeast Insertional Mutant collection contains over 3,600 mutagenizedyeast strains providing a set of gene disruption mutants for phenotypicanalysis. The mutagenized strains were developed via transposon inser-tional mutagenesis. The insertion of the transposon into the open readingframe (ORF) of the gene typically disrupts gene function, or insertionupstream or downstream of the ORF may result in mis-expression of thegene. The 6kb mTn contains the reporter gene lacZ, flanked by lox sites,and a 3xHA tag. The lacZ gene is lacking both its promoter and startcodon, thus, the expression is dependent on the transposon being in-serted in-frame into a gene and subsequently transcribed and trans-lated.

� Yeast Knockout Strains

The Saccharomyces Genome Deletion Project has developed a uniquecollection of knock-out strains covering 96% of the yeast genome. Thiscollection of over 6,000 gene-disruption mutants provides a unique toolfor the functional analysis of the yeast genome. The inclusion of distincttags - or “molecular barcodes” - that identify each strain allows pheno-typic analysis to be performed on a single gene basis or a genome-widescale. By using the yeast knock out strains for functional profiling, onecan also gather much information about human gene function by analogy.While >6000 represents the number of individual genes, there are fourbackgrounds (haploids of MATa and MATalpha, heterozygous diploid, andhomozygous diploid) with many genes available in more than one back-ground, thereby creating over 20,000 knock out strains.

� Yeast Magic Marker Strains

Genome-wide collections of yeast knockout (YKO) mutants have facili-tated the systematic functional analysis of yeast genes. However, haploidand homozygous diploid YKOs experience strong selection for compensa-tory mutations and so the genetic quality of such strains deterioratesover time. Knockout phenotypes can be protected from selection by“hiding” the mutation as a heterozygote with the wild-type allele. How-ever, it is then correspondingly difficult to detect the knockoutphenotype.The Magic Marker technology allows fresh creation of hap-loid knockouts from heterozygous diploids in a high-throughput mannerby employing a simple selection step following sporulation. Thus, a start-ing pool containing thousands of high-quality MATa haploid YKOs can beefficiently created.

� Yeast ORF Collection

The Yeast ORF Collection enables robust protein expression and purifica-tion for over 5500 S. cerevisiae genes. Each plasmid construct is avail-able in yeast or E. coli format as a glycerol stock.

• Open reading frame (ORF) clones provide a shortcut to

protein expression, allowing you to skip PCR, cloning into anexpression vector, and verifying the DNA sequence for eachconstruct.

• All yeast-format constructs have been verified by western

blotting to express protein of the correct length.

• The optional E. coli format enables easy conversion to

alternative yeast strains.

• The versatile 19-kDa tandem fusion tag enables a range of

detection and affinity purification strategies. In addition toProtein A and 6xHis domains, it contains a hemagglutination(HA) domain.

Yeast/ C.elegans cDNA Libraries and ClonescDNA LIBRARIES AND CLONES

Ready Clones


� Yeast TAP Fusion Collection

To facilitate global protein analyses, Dr. Erin O’Shea and Dr. JonathanWeissman (UCSF) have created a library where each ORF is tagged witha high-affinity epitope and expressed from its natural chromosomallocation. Through immunodetection of the common tag, a resource nowexists that provides a census of proteins expressed during log-phasegrowth and quantifies their absolute levels. The Yeast-TAP-Fusion Libraryallows the purification and selection of the entire yeast proteome andassociated components using two simple affinity selection steps in tandem,enabling the development of a range of high-throughput functional assays.The C-terminal TAP insertion cassette contains the coding region for amodified version of the TAP (Tandem Affinity Purification) tag, whichconsists of a calmodulin binding peptide, a TEV cleavage site and two IgGbinding domains of Staphylococcus aureus protein A, as well as a selectablemarker. Background strain = MatA (BY4741).

� Yeast Tet-Promoter Hughes Strains

The function of many of the essential genes has yet to be defined. In partthis is due to the difficulty of utilizing essential genes in functional as-says. The Tet-promoters Hughes collection (yTHC) from the Hughes Labo-ratory, University of Toronto, helps to overcome this barrier. The yTHCcollection contains 800 essential yeast genes for which expression is regu-lated by doxycycline. The endogenous promoter has been replaced witha Tet-titratable promoter in the genome. Thus, the expression of thegene can be switched off by the addition of doxycycline to the yeast’sgrowth medium.

� Incyte cDNA Libraries

Please view page #

� FISH Mapped BAC Clones

The Cancer Chromosome Aberration Project (CCAP), a joint initiativesupported by the NCI and NCBI, aims to develop tools that assist in theanalysis of chromosomal aberrations associated with malignant transfor-mation. To meet this objective, CCAP generated a set of cytogeneticallyFISH-mapped BAC clones at a resolution of 1-2Mb across the humangenome. The BACs have been physically mapped to the genome usingsequence tag sites (STS) and ordered using genetic linkage and/or radia-tion hybrid mapping data. Additionally, these clones are FISH mappedonto pro-metaphase spreads to allow high-resolution placement, uniformcoverage of the chromosomes, and their precise positioning relative toone another. This resource is the first collection to effectively integratethe cytogenetic and sequence maps of the human genome, thus facilitat-ing the transition to molecular cytogenetics.

To find an individual BAC clone covering a particular region:Point your browser at: http://cgap.nci.nih.gov/Chromosomes/CCAP_BAC_Clones

� GenMap Human BAC Collection

A genome wide collection of evenly distributed mapped BAC clones hasbeen made available by Dr. Vivian Cheung’s lab at the University ofPennsylvania. Beginning with the RPCI-11 human male BAC library, STSmarkers were used as anchors to map the BACs to the genome, at ~1Mbresolution. A subset of these clones were used in the NCI CCAP projectand the results of the FISH analyses performed by the NCI CCAP on theseclones are available at both the GenMapDB and the CCAP web pages.

To find a BAC clone covering a particular region: Point your browser at:http://genomics.med.upenn.edu/genmapdb/

Yeast cDNA Libraries and BAC ClonescDNA LIBRARIES AND CLONES

cDNA/BAC Clones

� Disease-specific Human Mapped BAC Collections

Break points in chromosomes, the evidence of which are deletions, in-sertions, inversions and translocations, have highlighted the role of genesin neoplasia. It was not long after researchers visualized these breakpointsthat they identified non-random associations between these microscopiclesions and specific disease phenotypes.

Disease-specific probe sets from Open Biosystems allow you access tocollections of clones that span known breakpoints associated with a par-ticular disease. These clones can be used on metaphase and interphasespreads using single or dual color FISH, as well as CGH to detect largerduplications, amplifications or deletions.

Each set contains FISH mapped clones that have been precisely placed onhuman chromosomes at approximately 1-2 Mb intervals. This has enabledthe identification and selection of BACs that either flank or encompassknown breakpoints associated with cancer.

B-cell (IgH) Leukemia & Lymphoma BAC Set availableT-cell (TCR) Leukemia & Lymphoma BAC Set availableEwing’s Sarcoma BAC Set available

� Mere Mouse Mapped BAC Collection

Dr. Julie Korenberg’s laboratory at Cedars-Sinai Medical Center hascreated a resource of 156 FISH-mapped BAC clones spanning the mousegenome at an average resolution of 19 Mb. Forty-two of these clones arelinked to the centromeric and telomeric ends of the Whitehead/MITrecombinational maps, while the remaining 114 clones have been as-signed to single chromosome bands. This resource is the first collectionto effectively integrate the cytogenetic and functional maps of the mousegenome. All clones were derived from the CITB mouse BAC library whichwas constructed from CJ7/129SV embryonic stem (ES) cells. All clonesare in the pBeloBAC11 vector and the average insert size for the libraryis 130Kb. Orders for individual clones must be placed by BAC clone ID.

� CalTech Human and Mouse BAC Clones


In-vitro Transcription, RNA polymerasesIN VITRO TRANSCRIPTION

Prokaryotic Polymerases

� T7, T3, and SP6 RNA Polymerases

T7, T3, and SP6 RNA Polymerases produce defined RNA by in vitro

transcription of double-stranded DNA that is downstream of therespective RNA polymerase promoter. It has extremely high promoterspecificity and is the best value in phage RNA polymerases.

Applications

• RNA synthesized can be used as a hybridization probe, anti-

sense RNA, a ribozyme, a template for in vitro translation,as a precursor mRNA for splicing or other processingstudies, or to make dsRNA for RNA interference or genesilencing.

• Synthesis of RNA for nucleic acid amplification methods or

gene expression studies.A buffer package containing 5X Transcription Buffer and DTT may bepurchased separately.


T7905K T7 RNA Polymerase 50 U/µl 5,000 U



TM910K T7 RNA Polymerase 200 U/µl 10,000 U



TH925K T7 RNA Polymerase 1,000 U/µl 25,000 U


TU950K T7 RNA Polymerase 2,500 U/µl 50,000 U





S4905K SP6 RNA Polymerase 50 U/µl 5,000 U

S4950K SP6 RNA Polymerase 50 U/µl 50,000 U

SM910K SP6 RNA Polymerase 200 U/µl 10,000 U

BP1001 Transcription Buffer Package 5 ml(5X Transcription Buffer and 2.5 mlof 100 mM DTT.)

� E. coli RNA Polymerase Core Enzyme andHoloenzyme (Sigma-Saturated)

Both the Core Enzyme and the Holoenzyme preparations of E. coli RNAPolymerase are isolated from the rifampicin-sensitive strain BL21.EPICENTRE is the only company that offers purified Core Enzyme, whichhas no detectable sigma subunit, and 100% sigma-saturated (s70)-Holoen-zyme.

The Core Enzyme is useful in studying mechanisms of transcriptioninitiation, since it will not initiate specific transcription at promotersequences on bacterial or bacteriophage DNA due to a lack of sigmafactor. However, the Core Enzyme will generate a variety of nonspecifictranscripts. The sigma-saturated Holoenzyme is very efficient inspecifically transcribing a variety of double-stranded DNA templatescontaining promoters.


C90100 E. coli RNA Polymerase Core Enzyme 100 U



S90050 E. Coli RNA Polymerase Holoenzyme(Sigma-Saturated) 50 U



� Thermus Thermostable RNA Polymerase

Derived from the thermophilic bacterium, Thermus thermophilus,Thermus Thermostable RNA Polymerase is the only commercially-avail-able RNA polymerase that is stable and has optimal activity at tempera-tures above 65°C. Thermus RNA Polymerase resembles E. coli RNA poly-merase in subunit structure.

� Q-Beta Replicase

Q-Beta Replicase (Qß Replicase) is an RNA-directed RNA polymerase thatis responsible for replication of the coliphage Q-Beta RNA genome. Vari-ous aspects of the enzyme are presented in a review by Blumenthal andCarmichael.1It is composed of four subunits, one of which is encoded bythe Q-Beta phage and three by the E. coli host. EPICENTRE’s Q-BetaReplicase is purified from E. coli containing a plasmid that expresses thephage-encoded subunit. All four enzyme subunits are present in equalproportions.


T90050 Thermus Thermostable RNA Polymerase 50 U




QB501250 Q-Beta Replicase 1 U/µl* 250 U

*Enzyme only, 10x TA Reaction buffer and NTPs are available separately.


D7P9201K T7 R & DNA ™ Polymerase 50 U/µl 1000 U

D7P9205K T7 R & DNA ™ Polymerase 50 U/µl 5000 U

D6P9301K SP6 R & DNA ™ Polymerase 50 U/µl 1000 U

D6P9305K SP6 R & DNA ™ Polymerase 50 U/µl 5000 U

� T7 & SP6 R & DNA™ Polymerases


� MiniV™ In Vitro Transcription Kit

MiniV™ In Vitro Transcription Kit provides MiniV™ RNA Polymerase*(MiniV RNAP) and all other components for in vitro transcription of RNAusing single-stranded DNA (ssDNA) templates that are functionally linkedto a single-stranded bacteriophage N4 promoter. MiniV RNAP is a tran-scriptionally-active 1,106-amino acid domain of the N4 virion RNA poly-merase.

Since it lacks RNA strand-displacement or unwinding activity on RNA:DNAhybrids, MiniV RNAP requires E. coli Single-Stranded Binding Protein(EcoSSB Protein) to displace the RNA transcript from the DNA templatestrand for efficient in vitro transcription. Like other RNA polymerases,MiniV RNAP can also synthesize concatameric RNA by rolling circle tran-scription of circular single-stranded DNA templates. Phage N4 transcrip-tion promoters used by MiniV RNAP are characterized by conservedsequences and a 5-basepair stem, 3-base loop hairpin structure.

� RiboScribe™ RNA Probe Synthesis Kits

RiboScribe™ T7, T3, and SP6 Probe Synthesis Kits are convenient,reliable, and cost-effective kits for synthesis of high-specific-activityradioactive RNA probes for Southern and Northern blots, in situ

hybridization, RNase protection assays, and other applications.


MV41025 MiniV™ In Vitro Transcription Kit 25 rxns


RS71207 RiboScribe™ T7 RNA Probe Synthesis Kit 25 rxns

RS71203 RiboScribe™ T3 RNA Probe Synthesis Kit 25 rxns

RS71206 RiboScribe™ SP6 RNA Probe Synthesis Kit 25 rxns

Enzyme solution contains RNase inhibitor. Control DNA template alsoprovided.

� AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit

The AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit enables flexiblehigh-yield synthesis of aminoallyl-labeled RNA. The kit utilizes EPICENTRE’sAmpliScribe™ T7 high-yield in vitro transcription system to incorporate5-(3-aminoallyl)-UTP (AA-UTP) into the RNA transcript. Since AA-UTP isincorporated into RNA with the same efficiency as UTP, high yields ofaminoallyl-labeled RNA are obtained. The aminoallyl-RNA product canthen be reacted with amine-reactive biotinylation reagents or fluores-cent dyes to obtain biotin-labeled or fluorescently-labeled RNA, respec-tively. Biotin-X-X-NHS is available from EPICENTRE for making biotin-labeled RNA, and amine-reactive dyes, such as N-hydroxy-succinimidyl(NHS) esters of fluorescent dyes, are commercially available from manysources. This method for making non-radioactively-labeled RNA is muchmore efficient and much less expensive than direct incorporation offluorescent- or biotin-labeled NTPs into RNA during in vitro transcrip-tion.

Applications

• Production of non-radioactive RNA probes for any purpose,

including:

• - In situ hybridization experiments.

• - Blotting experiments.


AA50125 AmpliScribe™ T7 Aminoallyl-RNA 25 rxnsTranscription Kit

� AmpliScribe™ T7-Flash™ Transcription KitAmpliScribe™ T3-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ and AmpliScribe™ T3-Flash™ TranscriptionKits, EPICENTRE’s new in vitro transcription kits, produce more RNA in30 minutes than other in vitro transcription kits produce in two hours.AmpliScribe T7-Flash and T3-Flash Kits utilize proprietary enzyme for-mulations that enable the maximum possible yields of RNA from virtuallyany template. The template can be any sequence, whether long or short,that is downstream of the respective T7 or T3 RNA polymerase pro-moter, including sequences in linearized plasmids, cDNA, double-strandedoligos or PCR products.

Benefits

• Exceptionally high yields of full-length RNA. A 20-µl

reaction with 1 µg of control DNA template yields 160 to 180µg (8 to 9 mg/ml) of full-length 1.4-kb RNA in 30 minutes.

• Fast. When transcribing 1 µg of linearized plasmid DNA

template, a standard AmpliScribe T7-Flash or T3-Flash

reaction is complete in 30 minutes.

• High yields with both long and short templates - even

with templates smaller than 30 bp. There’s no need for aspecial kit to make short RNA. AmpliScribe T7-Flash andAmpliScribe T3-Flash Kits produce high yields of activeribozymes, short hairpin RNA (shRNA), and more.

• Reactions are easily modified to incorporate non-

radioactive, biotin, aminoallyl-labeled NTPs, or capanalogs.

• RNase inhibitor is included in the AmpliScribe T7-Flash

and T3-Flash Enzyme Solutions to ensure RNA integrity.

• Can be used for “Eberwine”-type RNA amplification

procedures. However, we recommend TargetAmp™Aminoallyl-aRNA Amplification Kits for amplification of mRNAfrom laser capture microdissection (LCM) or other smallsamples.


ASF3057 AmpliScribe™ T7-Flash™ Transcription Kit 5 rxns





Enzyme solution contains Rnase Inh. Control DNA template also provided.

� AmpliScribe™ T7, T3, and SP6 High YieldTranscription Kits

AmpliScribe™ T7, T3, and SP6 High Yield Transcription Kits are speciallyformulated to utilize high concentrations of NTPs that are inhibitory toother in vitro transcription systems. AmpliScribe High Yield Transcrip-tion reactions can produce >20-fold more full-length RNA transcript thanconventional in vitro transcription reactions.


AS2607 AmpliScribe™ T7 High Yield Transcription Kit 25 rxns




Make RNA transcript from ssDNA, Synthesize RNA probesIN VITRO TRANSCRIPTIONProbe synthesis, RNA Amplification


Capped and tailed RNAIN VITRO TRANSCRIPTION

Transcripts for Translation

� AmpliCap-Max™ T7 & T3 High Yield MessageMaker Kits

EPICENTRE’s new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High YieldMessage Maker Kits are specially formulated to produce the highestyield of 5'-capped RNA (m7G[5']ppp[5']G cap analog) from an in vitro

transcription reaction in the shortest reaction time.

The new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield MessageMaker Kits feature:

• Yields up to 60 µg of 5'-capped RNA per reaction.

• 30 minute reaction time.

• Up to 80% of the RNA is capped.

• An optimized AmpliCap-Max™ Cap/NTP PreMix, containing

m7G[5']ppp[5']G Cap Analog and NTPs.

• A separate vial of GTP for efficient production of long, 5'-

capped RNA.


ACM04037 AmpliCap-Max™ T7 High Yield Message 25 rxnsMaker Kit

ACM04033 AmpliCap-Max™ T3 High Yield Message 25 rxnsMaker Kit

� AmpliCap™ T7 & SP6 High Yield Message Maker Kits

AmpliCap™ T7 & SP6 High Yield Message Maker Kits are optimized toproduce high yields of RNA having a standard m7G[5']ppp[5']G capanalog on the 5'-end. Capping efficiencies approach 80%. Can be usedfor functional studies of heterogenous nuclear RNAs and viral RNAa.

Benefits

• Yield up to 45 µg and 35 µg of RNA from 1µg of T7 and SP6

control templates, respectively, in a 20-µl reaction in 2 hours.

• Capping efficiencies up to 80%.

• An optimized m7G[5']ppp[5']G Cap/NTP PreMix is provided

for ease of use and highest yields of capped RNA tran-scripts.

• Separate vial of GTP is provided for efficient production of

long, 5'-capped RNA.

• Formulated to utilize high concentrations of NTPs that are

inhibitory to conventional in vitro transcription kits.


AC0707 AmpliCap™ T7 High Yield Message 25 rxnsMaker Kit

AC0706 AmpliCap™ SP6 High Yield Message 25 rxnsMaker Kit

� MessageMAX™ T7 Capped MessageTranscription Kit

Grudzien et al. recently demonstrated that in vitro-synthesized luciferasemRNA capped with the symmetrical cap analog, m7Gp

4m7G, was trans-

lated in a rabbit reticulocyte lysate system > 3-fold more efficiently thanthe same mRNA capped with the standard m7Gp

3G cap analog. EPICENTRE’s

new MessageMAX™ T7 Capped Message Transcription Kit gives the high-est possible yield of capped RNA in just 30 minutes and, since it will havea 2-Way Cap, you should observe higher translational efficiencies usingthe 5'-capped RNA generated.

Since the new 2-Way Cap included in the MessageMAX™ T7 Capped MessageTranscription Kit is a symmetrical molecule , 100% of the caps in the RNAproduced are in the correct orientation, increasing translation efficiencyof the RNA.

If the capped RNA is to be used for translation studies, translationalefficiencies can be much higher if the mRNA is also polyadenylated,especially if the mRNA is intended for use in vivo following transfectionor microinjection.


MM50110 MessageMAX™ T7 Capped Message 10 rxnsTranscription Kit

Contents:

MessageMAX™ T7 Enzyme Solution (with added RNase Inhibitor),MessageMAX™ 10X Transcription Buffer, 2-Way™ Cap/NTP PreMix,20 mM GTP, RNase-Free DNase I, DTT, RNase-Free Water, and ControlTemplate DNA (linearized)

� Standard Cap, 2-Way™ Cap, ARCA Cap,Unmethylated Cap and Trimethyl Cap Analogs

When a Cap Analog is substituted for a portion of the GTP present in anin vitro transcription reaction, up to ~80% of the transcripts will have acap on their 5'-end. RNA prepared for microinjection into oocytes or fortransfection into eukaryotic cells should be capped.

Anti-reverse cap analogs (ARCAs), such as 3'-O-methyl-m7GpppG, werefound to result in synthesis of transcripts that were more efficientlytranslated in vitro because the O-methyl group only permitted them tobe incorporated in the correct orientation.


C31010 Standard Cap Analog, m7G[5']ppp 500 nmoles[5']G Solution

C61025 Standard Cap Analog, 1250 nmolesm7G[5']ppp[5']G Solution

C41210 2-Way™ Cap Analog, 500 nmolesm7G[5']pppp[5']m7G Solution

C50110 ARCA Cap Analog, 3'-O-Methyl-m7G 500 nmoles[5']ppp[5']G Solution

C32010 Unmethylated Cap Analog, 500 nmolesG[5']ppp[5']G Solution

C06005 Trimethyl Cap Analog, 250 nmolesm2,7,7G[5']ppp[5']G Solution

Quantities of Cap Analogs are sometimes stated in absorbance units. Oneabsorbance unit is equivalent to approximately 50 nmoles of Cap Analog.

� A-Plus™ Poly(A) Polymerase Tailing Kit

A-Plus™ Poly(A) Polymerase uses ATP as a substrate for template-inde-pendent addition of adenosine monophosphate to the 3'-hydroxyl terminiof RNA molecules. A-Plus Poly(A) Polymerase is encoded by an E. coli genethat has been cloned in a plasmid and overexpressed in an E. coli strain.

• Addition of a poly(A) tail to RNA synthesized in vitro in

order to increase the stability of the RNA and enhance itsability to be translated in vivo after transfection ormicroinjection into eukaryotic cells.

• Addition of a poly(A) tail to an RNA molecule or a mixture ofRNA molecules in order to provide a priming site forsynthesis of first-strand cDNA using a primer with poly(dT)on its 3'-end portion.


PAP5104H A-Plus™ Poly(A) Polymerase 400 UTailing Kit (4 U/µl)

Contents: A-Plus™ Poly(A) Polymerase, A-Plus™ 10X Reaction Buffer,10 mM ATP, and Sterile RNase-Free Water.


� Kool™ NC-45™ Universal RNA Polymerase Template

Kool™ NC-45™ Universal RNA Polymerase Templates are small (28-150 nucle-otide) circular single-stranded DNA (ssDNA) molecules. As observed inthe laboratory of Dr. Eric Kool, these DNA nanocircles can be efficientlytranscribed in vitro by a variety of DNA-dependent RNA polymerases(RNAP) by rolling circle mechanism. Bacterial RNAPs transcribe Kool™templates in the absence of sigma factors, permitting studies of RNApolymerization mechanisms and inhibitor effects on the core RNAP. Whenusing a Kool Template, RNA is produced in significant excess over thetemplate DNA. Real-time detection methods for rolling circle transcrip-tion include use of dyes that exhibit fluorescence enhancement uponnucleic acid binding, such as SYBR® Green I dye, SYBR® Gold or RiboGreen®

(Molecular Probes), or use of molecular beacons.

• Screening for bacterial DNA-dependent RNAP activity.

• Screening compounds for RNAP inhibitor activity.

• Preparation of concatamers of short RNA sequences

� Kool™ NC-45™ RNAP Activity & InhibitorScreening Kit

The Kool™ NC-45™ RNAP Activity & Inhibitor Screening Kit utilizes theKool™ NC-45™ Template to screen inhibitors of rolling circle transcrip-tion by bacterial polymerases. Compounds can be screened for inhibi-tion of E. coli RNAP or inhibitors can be assayed on an RNAP provided bythe user. The high product-to-template ratio of rolling circle transcrip-tion allows a variety of detection methods in addition to following incor-poration of radioactive nucleotides. End-point or real-time monitoringis possible with fluorescent dyes or with molecular beacons. The Kool NC-45 Screening Kit uses SYBR® Green I Dye (Molecular Probes) for real-timedetection of RNAP activity.

Benefits

• A rapid, simple and sensitive method to assay RNAPs.

• RNAP activity can be assayed without a promoter sequence,

or knowledge of the promoter sequence.

• Very high product-to-template ratio.

• Provides a rapid and simple method for assaying RNAP

activity in real time without post-processing.

• Easy to multiplex and automate for high-throughput

screening of RNAP enzymes or RNAP inhibitors.


KN411100 Kool™ NC-45™ Universal RNA 100 µlPolymerase Template (1 pmole/µl)

KNK49025 Kool™ NC-45™ RNAP Activity & 25 rxnsInhibitor Screening Kit

� Tagetin™ RNA Polymerase Inhibitor

Tagetin™ RNA Polymerase Inhibitor is the only compound known to po-tently and selectively inhibit RNA polymerase III from a variety of eu-karyotic organisms including mammalian cells, Saccharomyces cerevisiae,Drosophila melanogaster, Bombyx mori, and Xenopus laevis oocytes.Itstrongly inhibits Escherichia coli RNA polymerase and plant chloroplastRNA polymerase.Plant nuclear RNA polymerases I, II, and III are much lesssensitive to Tagetin Inhibitor. Phage-encoded RNA polymerases such asSP6 and T7 are also relatively insensitive.With both eukaryotic andprokaryotic RNA polymerases, the degree of inhibition is template-de-pendent.


T9705H Tagetin™ RNA Polymerase Inhibitor 20 U/µl 500 U

T9701K Tagetin™ RNA Polymerase Inhibitor 1000 U

T9702K Tagetin™ RNA Polymerase Inhibitor 2500 U

� Ribonucleoside-5´-Triphosphate Solutions

(RNTPs) 2´-Florine-2´-Deoxyribonucleoside-5´-

Triphosphate Solutions


R109AT ATP 10 mM 5 µmoles

RA02825 ATP 100 mM 25 µmoles

R109CT CTP 10 mM 5 µmoles

RC02825 CTP 100 mM 25 µmoles

R109GT GTP 10 mM 5 µmoles

RG02825 GTP 100 mM 25 µmoles

R109UT UTP 10 mM 5 µmoles

RU02825 UTP 100 mM 25 µmoles

R344NT One Premixed Solution of all 4 NTPs 5 µmoles(Premix in 1 tube contains ATP, CTP, eachGTP, and UTP)

RN02825 One Tube of each of 4 NTPs 25 µmoles(ATP, CTP, GTP, UTP) each

R2F110C 2´-Fluorine-dCTP (2´-F-dCTP) 50 mM 1 µmole

R2F110U 2´-Fluorine-dUTP (2´-F-dUTP) 50 mM 1 µmole

� DuraScribe™ T7 Transcription Kit

� D4uraScribe® SP6 Transcription Kit

The DuraScribe™ T7 Transcription Kit* provides many benefits for mak-ing RNA for use in RNA interference (RNAi). The DuraScribe T7 Tran-scription Kit produces 2'-Fluorine-modified RNA transcripts-calledDuraScript™ RNA-that are completely resistant to RNase A. DuraScriptRNA is more stable in storage and in all RNA applications and can be madeand used without the worry and time-consuming procedures normallyrequired for keeping RNA transcripts intact. Recent research indicatesthat double-stranded DuraScript RNA can be delivered into culturedmammalian cells in the presence of serum and without the need fortransfection reagents. Double-stranded oligonucleotides, linearized plas-mids, or PCR products with a T7 promoter can be transcribed in aDuraScribe transcription reaction.

The DuraScribe™ T7 RNA Polymerase, provided in the kit, is a mutantform of T7 RNA Polymerase that efficiently incorporates 2'-Fluorine-CTP(2'-F-CTP) and 2'-Fluorine-UTP (2'-F-UTP), as well as ATP and GTP intofull length transcripts.


DS010910 DuraScribe™ T7 Transcription Kit 10 rxns

DS010925 DuraScribe™ T7 Transcription Kit 25 rxns

DS041010 DuraScribe® SP6 Transcription Kit 10 rxns

Rolling Circle transcription, durable transcripts, rNTPsIN VITRO TRANSCRIPTION

In-vitro Transcription

� Eukaryotic RNA Polymerases



MicroArray Analysis

RNA isolationRNA

Amplification

MicroArrayTarget Labeling

MicroArrayValidation

MasterPure™ RNAPurification Kit

ArrayPure™ Nano-Scale RNAPurification Kit

MasterPure™Yeast RNAPurification Kit

mRNA-ONLY™Eukaryotic mRNAIsolation Kit

mRNA-ONLY™ProkaryoticmRNAIsolation Kit

1-Round RNAAmplification

TargetAmp™ 1-RoundAminoallyl-aRNAAmplificationKit 101

TargetAmp™ 1-RoundAminoallyl-aRNAAmplification Kit102

TargetAmp™ 1-Round aRNAAmplificationKit 103

2-Round RNAAmplification

TargetAmp™ 2-RoundAminoallyl-aRNAAmplificationKit 1.0

TargetAmp™ 2-Round aRNAAmplification Kit2.0

AmpliScribe T7Aminoallyl-RNATranscription Kit

Biotin-X-X-NHS

MonsterScript™1st-Strand cDNASynthesis Kit

TAQurate™GREEN Real-TimePCR MasterMix

TAQurate™PROBES Real-Time PCRMasterMixe

FailSafe™PROBES Real-Time PCR System

FailSafe™ GREENReal-TimePCR System

MasterAmp™GREENReal-Time RT-PCR Kit

Procedures & ProductsMICROARRAY

Products available


Selection Guide for TargetAmp™ aRNA Amplification KitsMICROARRAY

Selection guide and Related Items

SuperScript™ III and SuperScript™ II reverse transcriptases(Invitrogen) are not included in the above TargetAmp™ Kits.

� Kits for Isolation of mRNA

� mRNA-ONLY™ Prokaryotic mRNA Isolation Kit� mRNA-ONLY™ Eukaryotic mRNA Isolation Kit


� MasterPure™ RNA Purification Kit


� ArrayPure™ Nano-Scale RNA Purification Kit


� MasterPure™ Yeast RNA Purification Kit


� Aminoallyl-UTP


AAU5202 Aminoallyl-UTP @ 50 mM 2.5 µmoles

AminoallylAntisense RNA

TargetAmp™2-Round

Aminoallyl-aRNAAmplification

Kit 1.0

TargetAmp™2-Round aRNAAmplification

Kit 2.0

TargetAmp™1-Round


Kit 101

TargetAmp™1-Round


Kit 102

TargetAmp™1-Round aRNA

Amplification Kit103

Name of Kit

Starting Total RNA

ReverseTranscriptasesUsed

Fold Amplificationof mRNA

Typical Yield ofAmplified aRNA(HeLa cells)

Total TimeRequired forAmplification

In vitroTranscription TimeRequired

End Product

10 pg - 500 pg

SuperScript™ III&

SuperScript™ II

5 X 106

1.5 µg - 70 µg

2 Days

4 hrs & 9 hrs

10 pg - 500 pg

SuperScript™ III&

SuperScript™ II

5 X 106

1.5 µg - 70 µg

2 Days

4 hrs & 9 hrs

Antisense RNA

25 ng - 500 ng

SuperScript™ III

5000

3 µg - 60 µg

1 Day

4 hrs


25 ng - 400 ng

6000

4 µg - 70 µg

1 Day

3 hrs

SuperScript™ III

5000

3 µg - 60 µg

1 Day

4 hrs

Antisense RNA

MonsterScript™


25 ng - 500 ng


� TargetAmp™ 1-Round Aminoallyl-aRNAAmplification Kit 101

TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101 provides allreagents (except Reverse Transcriptase) to achieve at least 5000-foldamplification of polyadenylated mRNA present in total RNA. The kit usesan improved “Eberwine”-type linear amplification method to generateaminoallyl- containing antisense RNA (AA-aRNA), also referred to cRNA,corresponding to mRNA in the sample. The fold amplification is sufficientto obtain AA-aRNA for use as labeled target for microarray analysis fromabout 2500 or more cells (25 ng total RNA). The entire procedure takesabout 2 hours of hands-on time and can be completed in one day, evenwith multiple samples.

• Produces microgram amounts aminoallyl-aRNA from 25 ng –

500 ng total RNA.

• Greater than 5000-fold amplification.

• Optimized for use with SuperScript™ III Reverse Tran-

scriptase (provided by the user).

• Simple 1-tube protocol can be completed in 1 day and

requires only about 2 hours of hands-on time

• Multiple RNA samples can be easily amplified simultaneously,

permitting high throughput.

• Reproducible with very low background

• Efficient protocols are available for labeling with Cy dyes,

Alexa dyes, biotin, or other labels for any oligo or cDNAarray format.

• TargetAmp kit 101 has been validated by the microarray

group of a leading pharmaceutical company for aRNA yield,amplification efficiency, AA-aRNA size and quality, repro-ducibility of transcript detection when cDNA was made fromthe mRNA to be amplified using SuperScript III (Invitrogen)Reverse transcriptase.

� TargetAmp™ 2-Round Aminoallyl-aRNAAmplification Kit 1.0

TargetAmp 2-Round Aminoallyly Kit 1.0 reproducibly detects moreexpressed genes, including low-abundance transcripts, that can bedetected using other RNA amplification kits.Applications: Amplification of ploy (A)- RNA from laser-captured cells,biopsy samples, cultured cells, or other minute cell samples formicroarray studies.

Benefits

• Produces microgram amounts of Aminoallyl-aRNA from 10

pg – 500 pg total RNA.

• Greater than 5,000,000-fold amplification.

• Optimized for use with SuperScript™ III & SuperScript™ II

Reverse Transcriptase (provided by the user).• Detects low abundance transcripts


TAA1R4910 TargetAmp™ 1-Round Aminoallyl-aRNA 10 rxnsAmplification Kit 101

TAA1R4924 TargetAmp™ 1-Round Aminoallyl-aRNA 24 rxnsAmplification Kit 101


TAA2R4910 TargetAmp™ 2-Round Aminoallyl-aRNA 10 rxnsAmplification Kit 1.0

TAA2R4924 TargetAmp™ 2-Round Aminoallyl-aRNA 24 rxnsAmplification Kit 1.0

� TargetAmp™ 1-Round aRNA Amplification Kit 103

This kit is similar to the TargetAmp 1-Round Aminoallyly-aRNA Kit 101except that TargetAmp Kit 103 includes only the four canonical ribo-nucleotides, ATP, CTP, GTP and UTP. Using the nucleotides provided,unlabeled aRNA is produced using an improved 1-day Eberwine-typelinear amplification process. This unlabelled aRNA will be amplified atleast 5000 fold compared to the amount of the mRNA in the total RNA usedin the reaction.

Applications:

• Generate unlabeled aRNA or aRNA with your own labelby amplification of polyadenylated mRNA in total RNAfrom small biological samples.

• Generate unlabeled aRNA for subsequent amplificationby RT-PCR for other applications.


TAU1R5110 TargetAmp™ 1-Round aRNA 10 rxnsAmplification Kit 103

TAU1R5124 TargetAmp™ 1-Round aRNA 24 rxnsAmplification Kit 103


TAU2R5110 TargetAmp™ 2-Round aRNA 10 rxnsAmplification Kit 2.0

TAU2R51224 TargetAmp™ 2-Round aRNA 24 rxnsAmplification Kit 2.0

� Biotin-X-X-NHS

Biotin-X-X-NHS is a useful reagent for labeling aliphatic amines for sub-sequent detection using avidin or streptavidin conjugates of fluorescentdyes or of enzymes that generate fluorescent or chemiluminescent prod-ucts. The compound provides an easy and efficient reagent for labelingaminoallyl-derivatized RNA or aRNA obtained using various RNA amplifi-cation procedures, including that obtained using EPICENTRE’s TargetAmp™Aminoallyl-aRNA Amplification Kits. Biotin-X-X-NHS can also be used tobiotinylate DNA or RNA that contains nucleotides with aminoallyl- or othergroups with reactive amines for use as nonradioactive probes forNorthern, Southern, or other hybridization experiments. In addition tolabeling, the addition of biotin to a biomolecule can provide a methodfor immobilizing or capturing the molecule using a streptavidin or avidinconjugate. Biotin-X-X-NHS can also be used to biotinylate reactive aminesin proteins. A protocol for labeling aminoallyl-RNA is provided with theproduct.

� TargetAmp™ 2-Round aRNA Amplification Kit 2.0

This kit provides all reagents (except Reverse Transcriptase) for gener-ating antisense RNA by amplifying polyadenylated mRNA present in totalRNA from biological samples. This is identical to TargetAmp 2-RoundAminoallyly Kit 1.0 except that it contains only the four canonical ribo-nucleotides ATP, CTP, GTP and UTP. The unlabelled RNA will be amplified5-million-fold.Therefore it works on samples of minute size such as 1-50cells.


BXX51005 Biotin-X-X-NHS 2.5 mg/Vial 5 Vials

BXX51010 Biotin-X-X-NHS 2.5 mg/Vial 10 Vials

TargetAmp™ Aminoallyl aRNA Amplification KitsMICROARRAYAmplification Kits


☛☛☛☛☛ Expression Arrest shRNAmir controls

Changes in the mRNA or protein levels in cells treated with negative ornon-silencing controls reflect non-specific responses in cells and canbe used as a baseline against which specific knockdown can be measured.

☛☛☛☛☛ Available Expression Arrest shRNAmir controls:

• Non-silencing shRNAmir construct

• Exogenous positive controls

• eGFP shRNA construct

• Luciferase shRNA construct.

• βββββ -gal reporter

� Mammalian RNAi

� shRNAmir libraries

shRNAmir triggers for mammalian RNAi are based on current knowledgeof the endogenous microRNA processing pathway. shRNAmir constructs aredesigned to mimic a natural microRNA primary transcript, enablingspecific processing by the endogenous RNAi pathway and producing moreeffective knockdown. In addition each gene-specific shRNAmir sequencehas been selected based on thermodynamic criteria for optimal smallRNA performance. The Expresion Arrest shRNAmir libraries incorporateseveral features aimed at increasing the efficiency and specificity ofgene knockdown and provide solutions for diverse RNAi applications.

Unique features include:

• microRNA-30 adapted design greatly increases knockdown

specificity and efficiency

• Human and mouse genome-wide coverage

• Already cloned into retroviral and lentiviral vectors

• Molecular barcodes for multioplexed screening

• Transient, stable and in vivo options for RNAi

• Guaranteed knockdown

☛☛☛☛☛ shRNA libraries

The RNAi consortium (TRC) is a collaborative effort based at the BroadInstitute of MIT and Harvard. Over three years TRC will create lentiviralshRNA libraries targeting 15,000 human (TRC-Hs 1.0) and 15,000 mouse(TRC-Mm1.0) annotated genes with a goal of validating each shRNA inmultiple cell lines. Currently the TRC lentiviral shRNA libraries consist ofapproximately 48,000 human shRNA constructs targeting 9700 humangenes and 16,572 mouse shRNA constructs targeting 3300 mouse genes.Additional releases will occur every quarter.

☛☛☛☛☛ RNAintro shRNAmir transfection kits

The RNAintro shRNA transfection kits combine the versatility and speci-ficity of Expression Arrest shRNAmir, the simplicity of validated controls,and optimized transfection reagent- all in a guaranteed to work pack-age.

Each RNAintro kit includes:

• Two shRNAmir constructs- Your choice from the human and

mouse genomes

• Arrest-In transfection reagent for shRNA delivery

• Positive controls- Luciferase or eGFP shRNA

• Negative control- Non silencing shRNA construct

• Transfection efficiency control- β gal reporter

☛☛☛☛☛ MicroRNA-adapted Retroviral Vectors

A series of microRNA-adapted retroviral vectors have been recentlydeveloped in the laboratory of Scott Lowe at CSHL to enable the efficientexpression of shRNAmir constructs from RNA Polymerase II (PolII) promoters. These vectors produce highly efficient knockdown evenwhen present at single copy in the genome. Since Pol II transcribesendogenous primary microRNA transcripts, the improved performanceof these vectors seems to derive from natural mechanisms of RNA-de-pendent gene inhibition.

Tetracycline-regulated retroviral vector- SIN-TREmiR30-PIG(TMP)

• MSCV-based self-inactivating (SIN)retroviral vector

• Express shRNAmir from tetracycline-regulated Pol II

promoter (TRE-CMV)

• Regulated gene knockdown in tet-on or tet-off configura-

tions.

• Select stableintegrants using Puromycin resistance

• GFP serves as amarker of shRNA expression

☛☛☛☛☛ Non-Mammalian RNAi

☛☛☛☛☛ Drosophila RNAi library

The collection consists of dsDNAs covering 50% of the Drosophila genome.For each gene, 300-600 bases of exonic sequence was amplified by genespecific primers. The dmRNAi constructs contain dual T7 promoters,and are RNase free, making them ready for direct use in in vitro tran-scription.

☛☛☛☛☛ Zebrafish Morpholinos


☛☛☛☛☛ C.elegans ORF-RNAi library

The C. elegans ORF-RNAi Library includes clones containing full openreading frames for over 11,000 genes, ready for your RNAi screens!These clones are derived from the C. elegans ORFeome Library v1.1.

• Full Coding Regions: The entire open reading frame is cloned

into an RNAi feeding vector and compatible host.

• Feeding Ready: The cloned ORFs have been transformed into the

RNAi feeding bacterial strain HT115(DE3)2 and the feeding vector,

PL4440.

• RNAi Versatility: Plasmid preps of the ORF-RNAi clones can be

used as templates for in vitro dsRNA synthesis for soaking or

injection

The C. elegans ORFeome clones that have been transferred into theRNAi feeding vector have been end sequence verified (Reboul 2003).A sampling of 100 RNAi clones have been randomly picked and sequenced.

☛☛☛☛☛ Arrest-In transfection reagent

Arrest-In™ transfection reagent is a proprietary lipo-polymeric formu-lation, developed and optimized for transfection of shRNA plasmid DNAinto the nucleus of cultured eukaryotic cells. Arrest-In™ transfectionreagent also provides an enhanced uptake efficiency of the shRNA plas-mid DNA into cells. Once in the cells Arrest-In™ promotes the entry of theshRNA containing plasmid into the nucleus where it is transcribed into ahairpin, enters the cytoplasm, is processed by the endogenous RNAimachinery into functional siRNAs.

MSCV/LTRmiR30-PIG (LMP)

RNAi Libraries/ Transfection kits/VectorsRNAi

shRNA Libraries, Vectors, Transfection kits


MessageMuter™ shRNA Production Kit

RNA interference (RNAi) is a powerful technique for elucidation of genefunction. Short hairpin RNA (shRNA) produced by chemical synthesis, invitro transcription, or expressed in vivo, has been shown to silencegenes as effectively as short dsRNAs.

The MessageMuter shRNA Production Process

The MessageMuter shRNA Production Kit utilizes a simple and unique 3-step process (Figure 1) that yields transfection-ready shRNA in about4 hours. A 17-base T7 Promoter Oligo is annealed to a DNA oligodesigned by the user using detailed instructions provided. The ends ofthe annealed duplex are “filled-in” using the Exo-Minus Klenow toobtain a linear dsDNA with a T7 RNAP promoter at its 5'-end. Followingin vitro transcription, the RNA produced spontaneously forms ahairpin structure (shRNA). Following clean-up, the shRNA is ready fortransfection into cultured cells. The MessageMuter shRNA kit providesreagents to produce 10 shRNAs in sufficient quantities for hundreds ofRNAi experiments.

Applications

• Production of shRNA for RNA Interference, micro RNA

studies, or other applications.

Benefits

• Produces shRNA in vitro using one user-designed oligo and

one in vitro transcription reaction.

• No need for annealing of sense and anti-sense RNA strands asrequired by other in vitro transcription-based methods.

Figure 1. Overview of the method used to produce shRNA usingthe MessageMuter™ shRNA Production Kit. All reagents are suppliedexcept a 60- to 76-base DNA oligo designed by the user as specified in thekit.

Contents:

T7 Promoter Oligo, 5X Annealing Buffer, Klenow exo- DNA Polymerase,dNTPs, 10X “Fill-In” Buffer, AmpliScribe™ T7-Flash, Enzyme Mix,AmpliScribe™ T7-Flash 10X Reaction Buffer, NTPs, DTT, Control luc21-Oligo, RNase-Free Dnase, Sterile Water


MM031110 MessageMuter™ shRNAi 10 rxnsProduction Kit

Make shRNA/ DNA-Binding ProteinsRNAi/MODIFYING ENZYMES

shRNA production / DNA-Binding Proteins

RecA Protein, E. coli

RecA Protein is a DNA-binding protein encoded by E.coli that playsintegral roles in both homologous recombination and post-replicativeDNA repair mechanisms. Initially, the protein binds preferentially tosingle-stranded DNA forming a nucleoprotein filament. The filamentcomplex binds to naked duplex DNA and searches for regions ofhomology. Once a region of homology is found, strand displacementand exchange begins.

Applications

• Site-directed mutagenesis through displacement loop

structures.

• Targeted site-specific cleavage of small and large DNA.

• Enrichment of target sequences from libraries or other DNA

pools.


RC44200 RecA Protein, E. coli (5 µg/µl) 200 µg

RC441MG RecA Protein, E. coli (5 µg/µl) 1 mg

Single-Stranded DNA Binding Protein (SSB), E. coli

E. coli Single-Strand DNA Binding Protein (SSB) binds single-strandedDNA with high specificity. In vivo, the protein is involved in DNA replica-tion, recombination, and repair. In vitro, SSB enhances several molecu-lar biology applications by destabilizing DNA secondary structure andincreasing the processivity of polymerases.

Applications

• Transcription of ssDNA templates by MiniV™ RNAP.

• Targeting restriction endonuclease digestion to any RE site

in cloned single-stranded DNA.

• Enhance the specificity and yield of PCR reactions.

• Improve DNA sequencing results through regions with strong

secondary structure.

• Site-directed mutagenesis in conjunction with recA protein.

• DNA replication and recombination studies.


SSB02200 Single-Stranded DNA Binding 200 µgProtein (SSB) (2mg/ml)

DNA Topoisomerase I, Vaccinia

Topoisomerase I from vaccinia virus is a type I eukaryotic topoisomerasethat removes both positive and negative superhelical turns (also calledright- and left-handed supercoils) from covalently closed DNA. The productof the reaction is a covalently closed, circular DNA with fewer positive ornegative superhelical turns.

Applications

• Studying the effects of supercoiling on transcription in

vitro.

• Studying chromatin reconstitution in vitro.

• Detecting mutant plasmids that differ in length by only one

basepair.


VT710500 DNA Topoisomerase I, Vaccinia (10 U/µl) 500 U

VT7101K DNA Topoisomerase I, Vaccinia (10 U/µl) 1000 U

VT7105K DNA Topoisomerase I, Vaccinia (10 U/µl) 5000 U


DNA Endonucleases & ExonucleasesMODIFYING ENZYMES

Nucleases

☛ DNA ENDONUCLEASES

☛ RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in re-moving DNA that might interfere with the characterization, manipula-tion, or use of RNA, or for any application requiring highly purifiedDNase I. It efficiently hydrolyzes double- and single-stranded DNA to amixture of short oligo- and mononucleotides.


D9902K RNase-Free DNase I (1U/µl) 2500 MBU



☛☛☛☛☛ Lambda Terminase

Lambda Terminase is an endonuclease encoded by bacteriophage lambdathat recognizes and cleaves DNA at cos sites, generating 5'-overhangs of12 bases in length. A typical digestion requires only 1 U of LambdaTerminase for each microgram of DNA.

Applications

• Generation of restriction maps of cosmid or fosmid clone

inserts.

• Linearization of cos site-containing clones for in vitro

packaging.

• Specific cleavage of chromosomal DNA for physical mapping.


LT4450 Lambda Terminase (2 U/µl) 50 U

LT44200 Lambda Terminase (2 U/µl) 200U

*Includes 10X Reaction Buffer and 10 mM ATP

☛ Endonuclease IV, E. coli

Endonuclease IV, cloned from the E. coli nfo gene, is a metalloenzymethat functions in vivo to repair free radical damage in DNA. The enzymealso has Class II abasic endonuclease activity, which has utility in manyareas of DNA damage and repair research.Endonuclease IV is also usefulin the study of the effects of anti-tumor drugs such as bleomycin onnucleic acids in vivo.


E70100 Endonuclease IV, E. coli (2 U/µl) 100 U

☛☛☛☛☛ T4 Endonuclease V

T4 Endonuclease V has N-glycosylase and apurinic/apyrimidinic lyase (APlyase) activities. Ultraviolet (UV) light produces covalent photoproductsin DNA, the most prevalent being a cis-syn cyclobutane pyrimidine dimer.T4 Endonuclease V locates and binds to pyrimidine dimers in double-stranded DNA. The enzyme then cleaves the N-glycosylic bond of the 5'-pyrimidine of the dimer and breaks the phosphodiester bond 3' to theresulting abasic site.


TE6605 T4 Endonuclease V (20 U/µl) 500 U

TE661K T4 Endonuclease V (20 U/µl) 1000 U

TE665K T4 Endonuclease V (20 U/µl) 5000 U

☛☛☛☛☛ DNA EXONUCLEASES

☛☛☛☛☛ Exonuclease III, E. coli

Exonuclease III digests duplex DNA in a 3'→¨ 5' direction from a bluntend, 5'-overhang or nick, producing stretches of single-stranded DNA.

Because the rate of exonucleolytic excision of deoxyribonucleotides byExonuclease III is dependent upon reaction factors including tempera-ture, ionic strength, template sequence, and enzyme-to-DNA ratios, eachtemplate must be optimized using sample digestions to achieve the de-sired excision rate.

☛☛☛☛☛ Exonuclease I, E. coli

Exonuclease I digests single-stranded DNA in a 3'→5' direction. Althoughit requires the presence of magnesium and a free 3'-hydroxyl terminusfor activity, it is active under a wide variety of buffer conditions andcan be added directly into most reaction mixes. Exonuclease I can beheat-inactivated by incubation at 80°C for 15 minutes.

☛☛☛☛☛ Exonuclease VII

Exonuclease VII has high enzymatic specificity for single-stranded DNAand exhibits both 5' → 3' and 3' → 5' exonuclease activities. It is espe-cially useful for rapid removal of single-stranded oligonucleotide prim-ers from a completed PCR reaction when different primers are re-quired for subsequent PCR reactions. Exonuclease VII digestion of single-stranded DNA occurs in the absence of magnesium.

☛☛☛☛☛ Lambda Exonuclease

Lambda Exonuclease is a highly processive 5'→¨3' exodeoxyribonucleasethat selectively digests the phosphorylated strand of double-strandedDNA. The preferred substrate is blunt-ended, 5'-phosphorylated double-stranded-DNA. The enzyme has reduced activity against nicked DNA andagainst single-stranded DNA and gapped DNA.


EN510100 Exonuclease VII (10 U/µl) 100 U

EN510250 Exonuclease VII (10 U/µl) 250 U


X40501K Exonuclease I, E. coli (20U/µl) 1000 U




EX4405K Exonuclease III, E.coli (200 U/µl) 5000 U

EX4425K Exonuclease III, E.coli (200 U/µl) 25000 U


LE035H Lambda Exonuclease (10 U/µl) 500 U

LE032K Lambda Exonuclease (10 U/µl) 2500U


DNA Exonucleases/RNA EndonucleasesMODIFYING ENZYMES

Nucleases

☛☛☛☛☛ RecBCD Nuclease, E. coli

RecBCD Nuclease is an exonuclease from E. coli that degrades single- anddouble-stranded DNA. Hydrolysis of the DNA is bi-directional from boththe 3' and 5' ends and processive, producing nucleoside monophosphates.Magnesium is required for the exonuclease activity, while calcium, nickel,zinc, and copper inhibit exonuclease activity. Calcium allows double-stranded DNA unwinding (helicase activity) without hydrolysis.

Applications

• Removal of contaminating bacterial chromosomal DNA in

plasmid, fosmid, cosmid, and BAC clone or vector prepara-tion.


BCD0401K RecBCD Nuclease, E. coli 1000 U

☛☛☛☛☛ Rec J Exonuclease

Rec J Exonuclease, derived from E. coli, catalyzes removal ofdeoxyribonucleoside monophosphates from single-stranded DNA in a 5' →3' direction. Its activity is dependent on Mg+2. Rec J Exonuclease can beheat-inactivated by incubation at 65°C for 20 minutes.

Applications

• Removes primers from completed PCR reactions.

• Degrades single-stranded linear DNA in dsDNA and plasmid

preps.


RJ411050 Rec J Exonuclease (10 U/µl) 50 U

RJ411250 Rec J Exonuclease (10 U/µl) 250 U

Includes 10X Reaction Buffer.

☛ T5 Exonuclease

T5 Exonuclease is a highly efficient 5' → 3' exonuclease for either single-stranded or duplex DNA. It has a tightly associatedsingle-strand-specific endonuclease activity when used in the presenceof 1-10 mM divalent magnesium ions. This activity may be selectivelysuppressed by using low concentrations of magnesium ions (< 1 mM),allowing nicked, double-stranded circular DNA to be “gapped” to a single-stranded circular species. The mode of action of T5 Exonuclease in vivo

may be analogous to that of the 5' → 3' exonuclease activity of E. coli DNApolymerase I. In the absence of divalent metal cofactors, T5 Exonucleaseis able to bind to DNA with a single-strand arm adjacent to a duplex DNAregion.

Applications

• Plasmid mutagenesis methods.

• Oligonucleotide site-directed mutagenesis.

• Generation of plasmid-sequencing templates.

• Removal of denatured DNA from alkaline-based plasmid

purification procedures for improved cloning procedures.


T5E4111K T5 Exonuclease (10 U/µl) 1000 U

☛☛☛☛☛ RNA ENDONUCLEASES

☛☛☛☛☛ RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3 '-end of pyrimidine residues, formingoligoribonucleotides having 3'-terminal pyrimidine-3'-phosphates. Pyri-midine-3'-monophosphates are also released by RNase A cleavage of adja-cent pyrimidine nucleotides.

Applications

• Removal of RNA from DNA preparations.

• Removal of unhybridized regions of RNA from DNA-RNA or

RNA-RNA hybrids.


MRNA092 Ribonuclease A (5 mg/ml) 2 ml

☛☛☛☛☛ Hybridase™ Thermostable RNase H

EPICENTRE’s patented Hybridase™ Thermostable RNase H specificallydegrades the RNA in a DNA:RNA hybrid, without affecting DNA orunhybridized RNA. In contrast to E. coli RNase H, which is rapidly inac-tivated at 55°C, Hybridase RNase H is active at high temperatures. It hasoptimal activity above 65°C and can be used at temperatures up to95°C. The thermostability of the enzyme permits it to be used at tem-peratures that give the highest hybridization stringency for specificDNA:RNA heteroduplexes, maximizing sensitivity and selectivity whileminimizing background due to nonspecific hybridization.

☛☛☛☛☛ RNase H, E. coli

Ribonuclease H (RNase H) from E. coli is an endonuclease that specificallydegrades the RNA in an RNA:DNA hybrid, without affecting DNA orunhybridized RNA. It will not degrade double-stranded DNA or single-stranded nucleic acids. EPICENTRE’s RNase H is highly purified and suit-able for use in diagnostic probe research, as well as other applications.However, in contrast to Hybridase™ Thermostable RNase H, which can beused at temperatures up to 95°C, E. coli RNase H is rapidly inactivatedat 55°C.


R0601K Ribonuclease H, E. coli (10 U/µl) 1000 U

R0650H Ribonuclease H, E. coli (10 U/µl) 5000 U


H39500 Hybridase™ Thermostable RNase H 500 U

H39100 Hybridase™ Thermostable RNase H 100 U


RNA Endonucleases/ DNA & RNA EndonucleasesMODIFYING ENZYMES

Nucleases

☛☛☛☛☛ RNase III, E. coli

Ribonuclease III (RNase III) from E. coli is an endoribonuclease thatspecifically digests double-stranded RNA (dsRNA) to dsRNA fragmentsthat have 2-base, 3'-overhangs.Complete digestion of dsRNA results indsRNA fragments of 12-15 bp.

☛ RNase T1, Aspergillus oryzae

Ribonuclease T1 (RNase T1) is an endoribonuclease that specifically cutsRNA or deaminated RNA at the 3'-end of guanosine residues and adjacentnucleotides through a 2', 3'-cyclic phosphate intermediate mechanism.EPICENTRE’s RNase T1 is cloned from Aspergillus oryzae and over ex-pressed in E. coli to produce a highly pure enzyme without contaminatingDNase or non-specific RNase activity.


RN02950 RNase III, E. coli (1 U/µl) 50 U


NT09100K RNase T1, Aspergillus oryzae 10,0000 U

NT09500K RNase T1, Aspergillus oryzae 50,0000 U

☛☛☛☛☛ RNase I, E. coli

RNase I degrades single-stranded RNA to nucleoside 3'-monophosphatesvia 2', 3' cyclic monophosphate intermediates by cleaving between alldinucleotide pairs, unlike RNase A, which cleaves only after cytosine anduridine. In addition, the enzyme is completely inactivated by heating at70°C for 15 minutes, eliminating the requirement to remove the enzymeprior to many subsequent procedures.


N6901K RNase I, E. coli (10 U/µl) 1000 U

N6905K RNase I, E. coli (10 U/µl) 5000 U

☛ RiboShredder™ RNase Blend

RiboShredder™ is a cocktail of potent RNases that completely degradesunwanted RNA in DNA purification procedures. This highly active cocktailcontains a proprietary optimized blend of non-mammalian RNase en-zymes. Unlike other RNase cocktails, RiboShredder RNase Blend degradesall RNA, converting RNA to nucleoside monophosphates by cutting be-tween all dinucleotide pairs.

Applications

• Removal of RNA from genomic and cloned DNA preparations.

Benefits• Completely degrades RNA rapidly.• DNase-free.


RS12100 RiboShredder™ RNase Blend 100 U

RS12500 RiboShredder™ RNase Blend 500 U

☛ DNA & RNA ENDONUCLEASES

☛ OmniCleave™ Endonuclease

OmniCleave™ Endonuclease is a highly purified enzyme from a recombi-nant E. coli strain that degrades single- and double-stranded DNA andRNA to di-, tri-, and tetranucleotides.


TER51020 Terminator™ 5´-Phosphate-Dependent Exonuclease (1 U/µl) 20 U


OC7810K OmniCleave™ Endonuclease (200 U/µl) 10000 U

OC7850K OmniCleave™ Endonuclease (200 U/µl) 50000 U

☛ DNA & RNA EXONUCLEASES

☛ Terminator™ 5'-Phosphate-Dependent Exonuclease

Terminator™ 5'-Phosphate-Dependent Exonuclease (Terminator Exonu-clease) is a 5'-to-3', processive exonuclease that degrades RNAs thathave a 5'-monophosphate – such as prokaryotic 16S and 23S rRNA andeukaryotic 18S and 28S rRNA. RNAs with a 5'-hydroxyl group, RNAs witha 5'-cap structure are resistant to degradation by Terminator Exonu-clease. Thus, Terminator Exonuclease is ideal for producing an mRNA-enriched sample from a total RNA preparation by selectively removingthe large rRNAs.

Terminator Exonuclease will also digest DNA with a 5'-monophosphate.Terminator Exonuclease is not inhibited by proteinaceous RNase inhibi-tors, such as RNasin® (Promega Corp) or other placental ribonucleaseinhibitors, or by Prime® Inhibitor (Eppendorf).

Applications

• Prepare mRNA-enriched samples from prokaryotic total RNA

preparations in 1 hour without the use of resins or magneticbeads.

• Prepare mRNA-enriched samples from eukaryotic total RNApreparations in 1 hour without the use of Oligo(dT).


M8202K Mung Bean Nuclease (50U/µl) 2000 U

M8205K Mung Bean Nuclease (50U/µl) 5000 U

Mung Bean Nuclease

Mung Bean Nuclease is a single-strand-specific nuclease purified fromsprouts of the mung bean Vigna radiata. Because Mung Bean Nuclease hashigher specificity for single-stranded DNA and RNA than S1 Nuclease, it isthe enzyme of choice for most applications requiring a single-strand-specific nuclease. Unlike S1 Nuclease, Mung Bean Nuclease will not cleavethe intact strand of nicked duplex DNA. Because of these characteris-tics, it is preferable to S1 Nuclease for many applications.


☛ Other Nucleases from Sibenzyme


E323 Endonuclease I 4000 U

E324 Endonuclease I 20,000 U

E341 Nuclease S1 200 U

E342 Nuclease S1 1000 U

345 Exonuclease III 4000 U

E346 Exonuclease III 20,000 U

☛ Nickase

Cat. No. Enzyme Recognition QtySequence

E402 N.Bst9 I - Nickase GAGTC(4/-) 500 U

☛ Other Nucleases from Jena Bioscience


EN-154L RNase T1 L Pack, (Recombinant, E. coli) 1,500,000 U

EN-154S RNase T1 S Pack, (Recombinant, E. coli) 300,000 U

EN-155L RNase T1 L Pack, (Recombinant, E. coli) 5 mg

EN-155S RNase T1 S Pack, (Recombinant, E. coli) 1 mg

EN-156L RNase TA L Pack, (Recombinant, E. coli) 5000 U

EN-156S RNase TA S Pack, (Recombinant, E. coli) 1000 U

EN-157L Exonuclease III L Pack (Recombinant, E. coli) 150,000 U

EN-157S Exonuclease III S Pack (Recombinant, E. coli) 30,000 U

EN-158L DNase Af L Pack (Recombinant, E. coli) 5 mg

EN-158S DNase Af S Pack (Recombinant, E. coli) 1 mg

EN-159 Exo/S1 Kit (ExoIII), 10,000/(S1 Nuclease), Recombinant, E. coli) 1000 U

Other Nucleases / Glycosylases / Excision MixesMODIFYING ENZYMES

Nucleases / Glycosylases

☛ Base-Specific DNA Excision Mixes and DNAGlycosylases

☛ 8-Oxoguanine-DNA Excision Mix

EPICENTRE’s 8-Oxoguanine-DNA Excision Mix is a blend of enzymes thatallows site-specific or random cleavage of DNA at oxidized guanine resi-dues. The positions of the oxidized guanine residues in a DNA sequencecan be mapped by sizing cleavage fragments on a sequencing-type gelfrom a fixed priming site, yielding data similar to a G-lane dideoxy-sequencing reaction.The first enzyme in the mix, 8-oxoguanine-DNAglycosylase depurinates oxidized G-residues. The second enzyme, T4Endonuclease IV, then cleaves the deoxyribose phosphate backbone atapurinic sites, generating a “polished” 3'-hydroxyl end and releasing aDNA fragment with an abasic 5'-phosphorylated end. The minimum oligo-mer size that will serve as a substrate for excision by the 8-Oxoguanine-DNA Excision Mix is 6 base pairs.

Applications

• Mapping of G residues in any DNA

• DNA repair studies


OG51100 8-Oxoguanine-DNA Excision Mix 100 rxns

☛ Uracil-DNA Excision Mix

EPICENTRE’s Uracil-DNA Excision Mix is a blend of enzymes that cleaveDNA at positions where uracil is present in place of thymine. The Uracil-DNA Excision Mix is useful for specific or random cleavage of DNA or forDNA repair studies, allowing mapping of uracil residues in any DNA.Uracil-DNA glycosylase in the Excision Mix removes uracil bases fromDNA, creating a single base gap and leaving the deoxyribose phosphatebackbone intact. Endonuclease IV in the Excision Mix then cleaves theDNA at each abasic site, leaving a 3´-hydroxyl end and an abasic 5´-phosphorylated end. The minimum oligomer size that will serve as asubstrate is 6 base pairs. Uracil-DNA Excision Mix digestion products canbe analyzed by denaturing agarose gel electrophoresis or denaturingpolyacrylamide gel electrophoresis.

Applications

• Mapping of uracil-containing residues in any DNA.

• Mapping CpG islands.

• DNA repair studies.


UEM04100 Uracil-DNA Excision Mix 100 rxns

☛ Glycosylase from Sibenzyme


E335 Uracil-DNA Glycosylase(UDG) 200 U

E336 Uracil-DNA Glycosylase(UDG) 1000 UCat. No. Product Qty

HU59100 HK™-UNG Thermolabile Uracil 100 UN-Glycosylase (1 U/µl)

HU5901K HK™-UNG Thermolabile Uracil 1000 UN-Glycosylase (1 U/µl)

Provided with Dilution Buffer

☛ HK™-UNG Thermolabile Uracil N-Glycosylase

HK™-UNG is a Uracil N-Glycosylase that can be easily heat-inactivated.Uracil N-Glycosylase (also known as uracil-DNA glycosylase) hydrolyzesthe N-glycosidic bond between the deoxyribose sugar and uracil in DNAcontaining deoxyuridine in place of thymidine. HK-UNG is active on bothsingle- and double-stranded DNA that contains uracil, but has no activityon RNA or 2'-deoxyuridine-5'-monophosphate. HK-UNG is ideal for study-ing repair of abasic sites in double-stranded DNA.

• HK-UNG is fully active at 50°C, but is inactivated by a 10-

minute incubation at 65°C.

Contents: 8-Oxoguanine-DNA Excision Enzyme Mix, Guanine OxidationReagent, and 10X 8-OxoG-DNA Excision Reaction Buffer.


PhosphatasesMODIFYING ENZYMES

Phosphatases

☛ Tobacco Acid Pyrophosphatase

Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acidanhydride bonds in the triphosphate bridge of the cap structure, re-leasing the cap nucleoside and generating a 5'-phosphorylated terminuson the RNA molecule.

Applications

• Preparation of templates for RACE (Rapid Amplification ofcDNA Ends).

• 5' and 3'-end mapping of RNA.

• Ligation of oligoribonucleotides to TAP-treated cellular RNAfor construction of full-length cDNA libraries.

• Mapping of transcription initiation sites for eukaryotic andprokaryotic transcripts.

• Radiolabeling of RNA for use in sequencing or as a hybridiza-tion probe.

☛ APex™ Heat-Labile Alkaline Phosphatase

APex™ Heat-Labile Alkaline Phosphatase is a new, recombinant enzymepreparation and is very pure and free from nuclease contamination.APex™ Phosphatase removes the 5'-phosphate from all types of DNA ends,including 5' protruding, blunt, and 5' recessed ends, and from RNA ends.The enzyme is irreversibly heat-inactivated by incubation at 70°C for 5minutes.

APex Phosphatase can be added directly to most restriction enzyme (RE)buffers and is highly active over a broad range of temperature (up to50°C), pH (from 5-12), and salt conditions (e.g., up to 1 M Na+, NH

4+,

K+, Cl- or Acetate-), and in the presence of 10% Triton X-100. To removethe 5'-phosphate from DNA, RNA or a nucleotide, simply incubate thesubstrate with APex Phosphatase at 37°C for 10 minutes.

Applications

• Dephosphorylation of DNA vectors prior to cloning to preventrecircularization.

• Preparation of 5'-nucleic acid termini for 5'-end labeling withpolynucleotide kinase.

• Dephosphorylation of DNA/RNA substrates for other purposes.


AP49010 APex™ Heat-Labile Alkaline 10 rxnsPhosphatase (1 rxn/µl)




T19050 Tobacco Acid Pyrophosphatase (TAP) 50 U




☛ HK™ Thermolabile Phosphatase

Dephosphorylation of 5’-protruding ends with HK Phosphatase preventsvector self-ligation and reduces the frequency of non-recombinant back-ground transformants during molecular cloning. The enzyme then can beeasily heat-killed prior to ligation reactions using the dephosphorylatedvector so that residual phosphatase will not remove the 5’-phosphategroups from DNA fragments to be cloned. However, HK Phosphatase isnot active in dephosphorylating recessed 5´-ends and has low activity onblunt ends. Further, the enzyme requires 5 mM divalent calcium cations,which are inhibitory to some restriction enzymes, so a separate 0.1 MCaCl

2 solution is provided for addition after the restriction endonuclease

digestion.

Applications

• Dephosphorylation of 5’-phosphates from protruding ends ofdsDNA for cloning or kinase end-labeling.

• Dephosphorylation of 5’-phosphates from RNA for kinaseend-labeling.


H92025 HK™ Thermolabile Phosphatase (1 U/µl) 25 MBU



Includes 10X TA Buffer and 0.1 M CaCl2

☛ NTPhos™ Thermolabile Phosphatase

NTPhos™ Thermolabile Phosphatase is a novel, recombinant enzyme thatis highly active in removing phosphate groups from the 5'-position ofribonucleoside- or 2'-deoxyribonucleoside-5'-triphosphates, diphosphatesor monophosphates. It can be used to remove 5'-phosphate groups fromnucleotide substrates following reactions using enzymes such as RNA-dependent or DNA-dependent RNA or DNA polymerases, terminal trans-ferases or poly(A) polymerases in order to prevent the nucleotides frominhibiting, interfering with, or causing background problems in subse-quent downstream reactions.

Following removal of 5'-phosphates from the nucleotides, the NTPhosenzyme can be completely and irreversibly heat-inactivated by incuba-tion at 65°C for 15 minutes.

Applications

• Removing 5'-phosphates from modified or unmodified NTPs,NDPs, NMPs, dNTPs, dNDPs, dNMPs, and inorganicpyrophosphate following in vitro reactions.

• Removing ATP from enzymatic reactions that use it as anenergy source.


NT4905H NTPhos™ Thermolabile Phosphatase 500 U(20 U/µl)

NT4910K NTPhos™ Thermolabile Phosphatase 10000 U(20 U/µl)

NT4920K NTPhos™ Thermolabile Phosphatase 20000 U(20 U/µl)


☛ T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-strandedDNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removesthe 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside3'-monophosphates, and deoxyribonucleoside 3', 5'-diphosphates to forma 3'-hydroxyl group.

Includes 10X Reaction Buffer without ATP. ATP is available separately.

☛ Ampligase® Thermostable DNA Ligase

Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligationof adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplexDNA structures that are stable at high temperatures. Derived from athermophilic bacterium, the enzyme is stable and active at much highertemperatures than conventional DNA ligases. This exceptional thermosta-bility permits extremely high hybridization stringency and ligation speci-ficity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids.

• Ligation Amplification (Ligase Chain Reaction, LCR) which

can distinguish between DNA sequences that differ by aslittle as a single base-pair and is a useful tool for detectionof single nucleotide polymorphisms (SNPs).

• Repeat Expansion Detection (RED), a ligation-based method

of genetic screening that detects DNA regions comprised ofmultiple nucleotide repeats.

• Simultaneous mutagenesis of multiple sites. Ampligase DNA

Ligase can introduce single or multiple point mutations atspecific sites by ordered ligation of PCR-amplified DNAfragments that have had point mutations introduced viamutant primers.

• Other ligation-based detection methods.


A8101 Ampligase® DNA Ligase Kit (5 U/µl) * 1000 U

A30201 Ampligase® DNA Ligase Kit (5 U/µl) * 5000 U

A0102K Ampligase® Enzyme and Buffer (100 U/µl) 2500 U

A32250 Ampligase® Enzyme and Buffer 5 U/µl) 250 U

A32750 Ampligase® Enzyme and Buffer (5 U/µl) 750 U

A3202K Ampligase® Enzyme and Buffer (5 U/µl) 2500 U

A0110K Ampligase® DNA Ligase (100U/µl) 10000 U

A0125K Ampligase® DNA Ligase (100U/µl) 25000 U

A3210K Ampligase® DNA Ligase (5 U/µl) 10000 U

A3225K Ampligase® DNA Ligase (5 U/µl) 25000 U

A1905B Ampligase® 10X Reaction Buffer 5 ml

A3201S Ampligase® 1X Storage Buffer 1 ml


P0505H T4 Polynucleotide Kinase, Cloned (10 U/µl) 500 U

P0501K T4 Polynucleotide Kinase, Cloned (10 U/µl) 1500 U

P0503K T4 Polynucleotide Kinase, Cloned (10 U/µl) 3000 U

Kinases/LigasesMODIFYING ENZYMES

Kinases/Ligases

*Contents : 1000/5000 U of Ampligase® DNA Ligase, 10X Reaction buffer& control DNA.

CircLigase™ ssDNA Ligase

CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase thatcatalyzes intramolecular ligation (i.e., circularization) of single-strandedDNA (ssDNA) templates having a 5'-phosphate and a 3'-hydroxyl group.

• Production of single-stranded DNA templates for rolling

circle replication or rolling circle transcription experi-ments.

• Production of single-stranded DNA templates for RNA

polymerase and RNA polymerase inhibitor assays.


CL4111K CircLigase™ ssDNA Ligase 1000 U

CL4115K CircLigase™ ssDNA Ligase 5000 U

Contents:

CircLigase™ ssDNA Ligase, CircLigase™ 10X Reaction Buffer, ATP, 50 mMMnCl

2, CircLigase™ Linear ssDNA Control Substrate, Water

Fast-Link™ DNA Ligation Kit

Fast-Link™ DNA Ligation Kit uses a high-quality ligase, called Fast-Link™DNA Ligase, that was cloned at EPICENTRE and then formulated to pro-vide extremely rapid high-efficiency DNA ligation. Cohesive-end liga-tions can be performed in 5 minutes at room temperature.

• Cohesive-end ligations in 5 minutes at room temperature.

• Blunt-end ligations in 15 minutes at room temperature.

• Ligation of PCR product with A-overhangs in 1 hour at 16°C.

• High ligation efficiency.

• Desalting of ligation products is not needed prior to

transformation of competent cells.


LK11025 Fast-Link™ DNA Ligation Kit 25 rxns.

LK0750H Fast-Link™ DNA Ligation Kit 50 rxns.

LK6201H Fast-Link™ DNA Ligation Kit 100 rxns.

Contents:

Fast-Link™ DNA Ligase, Fast-Link™ 10X Ligation Buffer, 10 mM ATP

E. coli DNA Ligase

E. coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the for-mation of phosphodiester bonds between complementary 3'-hydroxyland 5'-phosphoryl termini of double-stranded DNA. The enzyme worksbest with cohesive dsDNA ends and is also active on nicked DNA. Bluntends can be ligated in the presence of condensing reagents such as poly-ethylene glycol or Ficoll.


DL04082H E. coli DNA Ligase (10U/µl) 200 U

T4 RNA Ligase

T4 RNA Ligase catalyzes the formation of a phosphodiester bond betweena 5'-P-terminated nucleic acid donor and a 3'-OH nucleic acid acceptorin a template-independent manner. The enzyme is ATP-dependent, and isactive RNA, DNA, as well as numerous nucleotide derivatives.


LR5010 T4 RNA Ligase (5 U/µl) 1000 U




Restriction EnzymesRESTRICTION ENZYMES

Sibenzyme

Enzyme Prototype

E287 AatII AatII GACGT^C 500 U

E288 2500 U

E267 AauI Bsp1407I T^GTACA 500 U

E268 2500 U

E001 Acc16I MstI TGC^GCA 200 U

E002 1000 U

E289 Acc36I BspMI ACCTGC(4/8) 100 U

E290 500 U

E003 Acc65I (KpnI) G^GTACC 1000 U

E004 5000 U

E005 Acc113I ScaI AGTÂCT 600 U

E006 3000 U

E163 AccB1I HgiCI G^GYRCC 500 U

E164 2500 U

E179 AccB7I PflMI CCANNNN^NTGG 200 U

E180 1000 U

E007 AccBSI BsrBI GAG^CGG 1000 U

E008 5000 U

E011 AclI AclI AA^CGTT 200 U

E012 1000 U

E211 AclWI BinI GGATC(4/5) 100 U

E212 500 U

E013 AcsI ApoI RÂATTY 500 U

E014 2500 U

E213 AfeI Eco47III AGC^GCT 200 U

E214 1000 U

E173 AhlI SpeI A^CTAGT 1000 U

E174 5000 U

E015 AluI AluI AG^CT 400 U

E016 2000 U

E017 Ama87I AvaI C^YCGRG 1000 U

E018 5000 U

E019 ApaI ApaI GGGCC^C 1000 U

E020 5000 U

E235 AsiAI AgeI A^CCGGT 100 U

E236 500 U

E159 AsiSI SgfI GCGAT^CGC 200 U

E160 1000 U

E221 AspLEI HhaI GCG^C 500 U

E222 2500 U

E117 AspS9I Sau96I G^GNCC 1000 U

E118 5000 U

RecognitionSequence

Product

QtyCatNo.

☛ Restriction Enzymes

Enzyme Prototype

E257 AsuC2I CauII CC^SGG 2000 U

E258 10000 U

E231 AsuHPI HphI GGTGA(8/7) 200 U

E232 1000 U

E063 AsuNHI NheI G^CTAGC 500 U

E064 2500 U

E021 BamHI BamHI G^GATCC 4000 U

E022 20000 U

E023 Bbv12I HgiAI GWGCW^C 200 U

E024 1000 U

E025 BglI BglI GCCNNNN^NGGC 1000 U

E026 5000 U

E027 BglII BglII A^GATCT 1000 U

E028 5000 U

E029 Bme18I AvaII G^GWCC 1000 U

E030 5000 U

E457 BmtI NheI GCTAG^C 1000 U

E458 5000 U

E149 Bpu10I Bpu10I CC^TNAGC 200 U

E150 1000 U

E033 Bpu14I AsuII TT^CGAA 1000 U

E034 5000 U

E205 Bsa29I ClaI AT^CGAT 500 U

E206 2500 U

E219 Bsc4I BsiYI CCNNNNN^NNGG 500 U

E220 2500 U

E035 Bse1I BsrI ACTGG(1/-1) 500 U

E036 2500 U

E253 Bse3DI BsrDI GCAATC(2/0) 200 U

E254 1000 U

E147 Bse8I BsaBI GATNN^NNATC 1000 U

E148 5000 U

E037 Bse21I SauI CC^TNAGG 500 U

E038 2500 U

E039 Bse118I Cfr10I R^CCGGY 200 U

E040 1000 U

E181 BsePI BsePI G^CGCGC 200 U

E182 1000 U

E263 BseX3I XmaIII C^GGCCG 200 U

E264 1000 U

E285 Bso31I Eco31I GGTCTC(1/5) 100 U

E286 500 U

RecognitionSequence

Product

QtyCatNo.




Sibenzyme

E291 BsoMAI BsmAI GTCTC(1/5) 1000 U

E292 5000 U

E185 Bsp1720I EspI GC^TNAGC 100 U

E186 500 U

E245 BspA2I AvrII C^CTAGG 100 U

E246 500 U

E273 BssECI SecI C^CNNGG 200 U

E274 1000 U

E261 BssNAI SnaI GTA^TAC 1000 U

E262 5000 U

E207 BssT1I StyI C^CWWGG 1000 U

E208 5000 U

E239 Bst6I Ksp632I CTCTTC(1/4) 200 U

E240 1000 U

E043 Bst2BI BsiI CTCGTG(-5/-1) 200 U

E044 1000 U

E051 Bst2UI BstNI CC^WGG 1000 U

E052 5000 U

E265 Bst4CI Tsp4CI ACN^GT 200 U

E266 1000 U

E093 BstACI AcyI GR^CGYC 500 U

E094 2500 U

E259 BstAPI (ApaBI) GCANNNN^NTGC 200 U

E260 1000 U

E237 BstBAI BsaAI YAC^GTR 500 U

E238 2500 U

E305 BstC8I Cac8I GCN^NGC 200 U

E306 1000 U

E227 BstDEI DdeI C^TNAG 500 U

E228 2500 U

E083 BstDSI DsaI C^CRYGG 1000 U

E084 5000 U

E103 BstENI EcoNI CCTNN^NNNAGG 200 U

E104 1000 U

E119 BstENII MboI ^GATC 200 U

E120 1000 U

E031 BstF5I (Fok) GGATG(2/0) 500 U

E032 2500 U

E283 BstFNI FnuDII CG^CG 300 U

E284 1500 U

E171 BstH2I HaeII RGCGC^Y 500 U

E172 2500 U

RecognitionSequence

Product

QtyCatNo.


PrototypeEnzyme

E143 BstHHI HhaI GCG^C 2000 U

E144 10000 U

E077 BstHPI HpaI GTTÂAC 500 U

E078 2500 U

E151 BstKTI MboI GAT^C 200 U

E152 1000 U

E071 BstMCI McrI CGRY^CG 500 U

E072 2500 U

E251 BstNSI NspI RCATG^Y 200 U

E252 1000 U

E299 BstPAI PshAI GACNN^NNGTC 1000 U

E300 5000 U

E307 BstSCI ScrFI ^CCNGG 100 U

E308 500 U

E197 BstSFI SfeI C^TRYAG 200 U

E198 1000 U

E065 BstSNI SnaBI TAC^GTA 200 U

E066 1000 U

E303 BstV1I BbvI GCAGC(8/12) 100 U

E304 500 U

E297 BstV2I BbvII GAAGAC(2/6) 200 U

E298 1000 U

E229 BstX2I XhoII R^GATCY 500 U

E230 2500 U

E053 BsuRI HaeIII GG^CC 1000 U

E054 5000 U

E277 BtrI BtrI CACGTC(-3/-3) 50 U

E278 250 U

E203 CciNI NotI GC^GGCCGC 200 U

E204 1000 U

E055 DraI AhaIII TTTÂAA 500 U

E056 2500 U

E309 DraIII DraIII CACNNN^GTG 500 U

E310 2500 U

E241 DseDI DrdI GACNNNN^NNGTC 300 U

E242 1500 U

E057 EcoRI EcoRI GÂATTC 5000 U

E058 25000 U

E059 EcoRV EcoRV GATÂTC 1000 U

E060 5000 U

E243 EgeI (NarI) GGC^GCC 200 U

E244 1000 U

RecognitionSequence

Product

QtyCatNo.


PrototypeEnzyme



Sibenzyme

E061 ErhI StyI C^CWWGG 1000 U

E062 5000 U

E153 FalI FalI (8/13)AAG(N)5CTT(13/8) 100 U

E154 500 U

E155 FatI NlaIII ^CATG 50 U

E156 250 U

E209 FauI FauI CCCGC(N4/6) 50 U

E210 250 U

E009 FauNDI NdeI CA^TATG 500 U

E010 2500 U

E271 FblI AccI GT^MKAC 100 U

E210 500 U

E247 FokI FokI GGATG(9/13) 100 U

E248 500 U

E157 FriOI BanII GRGCY^C 1000 U

E158 5000 U

E095 Fsp4HI Fnu4HI GC^NGC 100 U

E096 500 U

E067 HaeIII HaeIII GG^CC 1000 U

E068 5000 U

E201 HindII HindII GTY^RAC 1000 U

E202 5000 U

E073 HindIII HindIII AÂGCTT 2000 U

E074 10000 U

E075 HinfI HinfI GÂNTC 1000 U

E076 5000 U

E161 HpaII HpaII C^CGG 500 U

E162 2500 U

E069 HspAI (HhaI) G^CGC 1000 U

E070 5000 U

E079 KpnI KpnI GGTAC^C 2000 U

E080 10000 U

E081 Ksp22I BclI T^GATCA 1000 U

E082 5000 U

E187 Kzo9I MboI ^GATC 200 U

E188 1000 U

E121 MabI SexAI A^CCWGGT 200 U

E122 1000 U

E049 MhlI SduI GDGCH^C 500 U

E050 2500 U

E085 MluI MluI A^CGCGT 1000 U

E086 5000 U

RecognitionSequence

Product

QtyCatNo.


PrototypeEnzyme

E189 Mly113I NarI GG^CGCC 200 U

E190 1000 U

E087 MroNI (NaeI) G^CCGGC 500 U

E088 2500 U

E249 MroXI XmnI GAANN^NNTTC 200 U

E250 1000 U

E091 MspI HpaII C^CGG 1000 U

E092 5000 U

E301 Msp20I BalI TGG^CCA 100 U

E302 500 U

E191 MspA1I NspBII CMG^CKG 500 U

E192 2500 U

E175 MspR9I ScrFI CC^NGG 500 U

E176 2500 U

E099 NruI NruI TCG^CGA 500 U

E100 2500 U

E193 NruGI Eam1105I GACNNN^NNGTC 100 U

E194 500 U

E105 PceI StuI AGG^CCT 1000 U

E106 5000 U

E275 PciI BspLU11I A^CATGT 200 U

E276 1000 U

E045 PctI BsmI GAATGC(1/-1) 500 U

E046 2500 U

E195 Ple19I PvuI CGAT^CG 100 U

E196 500 U

E269 PpsI PleI GAGTC(4/5) 25 U

E270 100 U

E279 PsiI PsiI TTA^TAA 100 U

E280 500 U

E453 Psp6I EcoRII ^CCWGG 100 U

E454 500 U

E107 Psp124BI SacI GAGCT^C 1000 U

E108 5000 U

E169 PspEI BstEII G^GTNACC 2000 U

E170 10000 U

E223 PspLI SplI C^GTACG 300 U

E224 1500 U

E089 PspN4I NlaIV GGN^NCC 1000 U

E090 5000 U

E215 PspOMI (ApaI) G^GGCCC 1000 U

E216 5000 U

RecognitionSequence

Product

QtyCatNo.


PrototypeEnzyme



Sibenzyme

E255 PspPPI PpuMI RG^GWCCY 100 U

E256 500 U

E131 PsrI PsrI (7/12)GAAC(N)6TAC(12/7) 100 U

E132 500 U

E109 PstI PstI CTGCA^G 4000 U

E110 20000 U

E111 PvuII PvuII CAG^CTG 2000 U

E112 10000 U

E113 RsaI RsaI GTÂC 1000 U

E114 5000 U

E281 Rsr2I Rsr II CG^GWCCG 200 U

E282 1000 U

E115 SalI SalI G^TCGAC 2000 U

E116 10000 U

E101 SbfI Sse8387I CCTGCA^GG 200 U

E102 1000 U

E165 SfaNI SfaNI GCATC(5/9) 100 U

E166 500 U

E123 SfiI SfiI GGCCNNNN^NGGCC 500 U

E124 2500 U

E125 Sfr274I XhoI C^TCGAG 2000 U

E126 10000 U

E127 Sfr303I SacII CCGC^GG 1000 U

E128 5000 U

E177 SmaI SmaI CCC^GGG 2000 U

E178 10000 U

E225 SmiI SwaI ATTTÂAAT 1000 U

E226 5000 U

E293 SmiMI MslI CAYNN^NNRTG 200 U

E294 1000 U

E129 SphI SphI GCATG^C 200 U

E130 1000 U

E217 Sse9I Tsp509I ÂATT 100 U

E218 500 U

E041 SspI SspI AATÂTT 500 U

E042 2500 U

E133 TaqI TaqI T^CGA 200 U

E134 1000 U

E199 Tru9I MseI T^TAA 200 U

E200 1000 U

E097 Tth111I Tth111I GACN^NNGTC 400 U

E098 2000 U

RecognitionSequence

Product

QtyCatNo.


PrototypeEnzyme

E143 BstHHI HhaI GCG^C 2000 U

E135 Vha464I AflII C^TTAAG 500 U

E136 2500 U

E137 VneI ApaLI G^TGCAC 1000 U

E138 5000 U

E139 VspI VspI AT^TAAT 1000 U

E140 5000 U

E141 XbaI XbaI T^CTAGA 1000 U

E142 5000 U

E233 XmaI XmaI C^CCGGG 100 U

E234 500 U

E463 ZraI (AatII) GAC^GTC 200 U

E464 1000 U

E145 Zsp2I NsiI ATGCA^T 1000 U

E146 5000 U

RecognitionSequence

Product

QtyCatNo.


PrototypeEnzyme



Jena Bioscience


EN-101L Alu I, L pack 3000 U

EN-101S Alu I, S pack 600 U

EN-102L Asu II, L pack 17500 U

EN-102S Asu II, S pack 3500 U

EN-103L BamH I, L pack 50000 U

EN-103S BamH I, S pack 10000 U

EN-104L Bcl I, L pack 12500 U

EN-104S Bcl I, S pack 2500 U

EN-105L Bgl I, L pack 10000 U

EN-105S Bgl I, S pack 2000 U

EN-106L Bgl II, L pack 6500 U

EN-106S Bgl II, S pack 1300 U

EN-107L BseA I, L pack 3250 U

EN-107S BseA I, S pack 650 U

EN-108L BseB I, L pack 22500 U

EN-108S BseB I, S pack 4500 U

EN-109L BseC I, L pack 4000 U

EN-109S BseC I, S pack 800 U

EN-110L BshF I, L pack 35000 U

EN-110S BshF I, S pack 7000 U

EN-111L BsiS I, L pack 11000 U

EN-111S BsiS I, S pack 2200 U

EN-112L BssA I, L pack 1500 U

EN-112S BssA I, S pack 300 U

EN-113L CspA I, L pack 750 U

EN-113S CspA I, S pack 150 U

EN-114L EcoR I, L pack 25000 U

EN-114S EcoR I, S pack 5000 U

EN-115L EcoR V, L pack 15000 U

EN-115S EcoR V, S pack 3000 U

EN-116L Hind III, L pack 40000 U

EN-116S Hind III, S pack 8000 U

EN-117L Hinf I, L pack 16500 U

EN-117S Hinf I, S pack 3300 U

EN-118L Hpa I, L pack 5000 U

EN-118S Hpa I, S pack 1000 U

EN-119L Kpn I, L pack 17500 U

EN-119S Kpn I, S pack 3500 U

EN-120L Mbo I, L pack 1500 U

EN-120S Mbo I, S pack 300 U

EN-121L MspC I, L pack 6500 U

EN-121S MspC I, S pack 1300 U

EN-122L Nae I, L pack 1750 U

EN-122S Nae I, S pack 350 U

EN-123L Nco I, L pack 3000 U

EN-123S Nco I, S pack 600 U

EN-124L Not I, L pack 1500 U

EN-124S Not I, S pack 300 U

EN-160L Dpn I, L-Pack 1250 U

EN-160S Dpn I, S-Pack 250 U



EN-125L Nru I, L pack 3500 U

EN-125S Nru I, S pack 700 U

EN-126L PspP I, L pack 4500 U

EN-126S PspP I, S pack 900 U

EN-127L Pst I, L pack 40000 U

EN-127S Pst I, S pack 8000 U

EN-128L Pvu II, L pack 22500 U

EN-128S Pvu II, S pack 4500 U

EN-129L Rsa I, L pack 5000 U

EN-129S Rsa I, S pack 1000 U

EN-130L Sal I, L pack 10000 U

EN-130S Sal I, S pack 2000 U

EN-131L Sca I, L pack 6000 U

EN-131S Sca I, S pack 1200 U

EN-132L Sfi I, L pack 2750 U

EN-132S Sfi I, S pack 550 U

EN-133L SgrB I, L pack 8000 U

EN-133S SgrB I, S pack 1600 U

EN-134L Sla I, L pack 25000 U

EN-134S Sla I, S pack 5000 U

EN-135L Sma I, L pack 5500 U

EN-135S Sma I, S pack 1100 U

EN-136L SnaB I, L pack 1750 U

EN-136S SnaB I, S pack 350 U

EN-137L Sph I, L pack 1250 U

EN-137S Sph I, S pack 250 U

EN-138L SseB I, L pack 10000 U

EN-138S SseB I, S pack 2000 U

EN-139L Ssp I, L pack 3000 U

EN-139S Ssp I, S pack 600 U

EN-140L Sst I, L pack 8000 U

EN-140S Sst I, S pack 1600 U

EN-141L Sty I, L pack 30000 U

EN-141S Sty I, S pack 6000 U

EN-142L Taq I, L pack 22500 U

EN-142S Taq I, S pack 4500 U

EN-143L Xba I, L pack 17500 U

EN-143S Xba I, S pack 3500 U

EN-144L BstE II, L pack 10000 U

EN-144S BstE II, S pack 2000 U

EN-145L Acc I, L pack 1500 U

EN-145S Acc I, S pack 300 U

EN-146L Nhe I, L pack 2750 U

EN-146S Nhe I, S pack 550 U

EN-170L BspLU4 I, L Pack 7500 U

EN-170S BspLU4 I, S Pack 1500 U

EN-171L BspLU11 I, L Pack 750 U

EN-171S BspLU11 I, S Pack 150 U

EN-150L Sau3A I, L pack 2500 U

EN-150S Sau3A I, S pack 500 U



The following examples of In Vitro Transposomics applicationsare discussed in this category:

• EZ-Tn5™<T7/KAN-2> Promoter Insertion Kit: Introduce a

phage T7 promoter into cloned DNA and generate RNAtranscripts in vitro or in vivo from the insertion site.

• EZ-Tn5™ <R6Kγori/KAN-2> Insertion Kit: Rapidly character-

ize plasmids that otherwise would not replicate in E.coli byrandomly inserting an E.coli conditional origin of replication(R6Kγ ori) and a kanamycin resistance marker.

• EZ-Tn5™ β-Lactamase Fusion Kit: Directly select genes

encoding cell envelope and secreted proteins by creatingtranslational fusions to β-lactamase.

• EZ-Tn5™ pMOD™ Transposon Construction Vectors: Use these

vectors to make a custom EZ-Tn5 Transposon having aselectable marker, reporter gene, control element, or anyother DNA sequence of interest.

☛ Criteria for Choosing a Transposon System

EPICENTRE has hyperactive Tn5-based EZ-Tn5™ Transposon Systems andhyperactive phage Mu-based HyperMu™ Transposon Systems for both invitro and in vivo applications. The properties of these and other com-mercial systems are summarized in the following table and importantcriteria for choosing between them are discussed below.

In Vitro Transposomics™: Endless PossibilitiesTRANSPOSOMICS

Insert Promoters, Origin of replication

*The “in vivo transposition frequency” data here refers to the relativenumber of colonies obtained containing transposon insertions followingelectroporation of electrocompetent E. coli cells with a synaptic complex(“Transposome™ Complex) formed between a transposon and therespective transposase.

** HyperMu™ MuA Transposase can insert transposons with Mu Ends havingfree 3'-termini into another DNA, but is unable to excise the transposonfrom another DNA.

EZ-Tn5 Systems have very high transposition efficiencies for in vitro

insertion, as well as for in vivo insertion of an EZ-Tn5 Transposome™Complex, the synaptic complex formed between an EZ-Tn5 Transposaseand an EZ-Tn5 Transposon. Although EPICENTRE’s HyperMu™ MuATransposase is at least 50 times more active for in vitro transpositionthan the MuA transposase from other suppliers and almost as active asthe EZ-Tn5 System in vitro, the HyperMu Transposome™ Complex generates10-100 times fewer insertions than the EZ-Tn5 System in vivo usingE. coli.

☛ Inserting a T7 RNA Polymerase Promoter toTranscribe from the Insertion Site

☛ EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit provides an easy andreliable method to randomly insert a transposon containing a phage T7RNA polymerase promoter into any DNA molecule in vitro. A simple twohour in vitro reaction randomly inserts a single EZ-Tn5™ <T7/KAN-2>Transposon into the plasmid, cosmid, fosmid or BAC clone. Then, follow-ing transformation into E. coli, transposon insertion clones are selectedon kanamycin plates. The insertion site of individual kanamycin-resistantinsertion clones can be mapped or sequenced bidirectionally using thetwo sequencing primers included in the kit. The transposon end has notermination sequences, so RNA can be produced from chosen insertionclones by in vitro transcription from the T7 RNA polymerase promoter.


EZI03T7 EZ::TN™ <T7/KAN-2>Promoter Insertion Kit* 10 rxns

TNP92110 EZ::TN™ Transposase 10 U

TK0701 EZ::TN™ <T7/KAN-2> Transposon 1 pmole

KFP9122 KAN-2 FP-1 Forward Primer 1 nmole

KRP0021 KAN-2 RP-1 Reverse Primer 1 nmole

*Contents:

EZ::TN™ Transposase, EZ::TN™<T7/KAN-2> Transposon, EZ::TN™ 10XReaction Buffer, EZ::TN™ 10X Stop Solution, Forward and ReversePrimer, Control Target DNA, Sterile Water

☛☛☛☛☛ Kit for Inserting Priming Sites and a Multi-Copy-Inducible oriV

☛ EZ-Tn5™ <oriV/KAN-2> Insertion Kit

The EZ-Tn5™ <oriV/KAN-2> Insertion Kit is designed to simplify andspeed up both DNA purification and sequencing of existing single-copyclones by integrating CopyControl™ capability. Use a short, one-step invitro reaction to randomly insert a single EZ-Tn5™ <oriV/KAN-2>Transposon containing an inducible, high-copy origin of replication (oriV)into the target DNA. Then, transform TransforMax™ EPI300™ or EPI300™-T1R Electrocompetent E. coli (sold separately) with an aliquot of thereaction and select for the kanamycin marker encoded by the EZ-Tn5Transposon.

One reaction will generate thousands of EZ-Tn5 <oriV/KAN-2> Transposoninsertion clones. Each clone contains primer binding sites at the ends ofthe inserted transposon for sequencing. Amplify selected clones by add-ing the CopyControl™ Induction Solution.


EZI02VK EZ::TN™ <oriV/KAN-2> Insertion Kit* 10 rxns


VK0231 EZ::TN™ <oriV/KAN-2> Transposon 1 pmole

URP023 TN RP-1 Reverse Primer 1 nmole

UFP024 TN FP-1 Forward Primer 1 nmole

*Contents:

EZ::TN™ Transposase, EZ::TN™ <oriV/KAN-2> Transposon, EZ::TN™ 10XReaction Buffer, EZ::TN™ 10X Stop Solution, Forward and ReversePrimer, Control Target DNA, Sterile water

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EZ-Tn5™

High High Shown ME, 19 9 1 (Tnp)

HyperMu™**

High Medium Shown -51 5 1 (MuA)

Tn7-Type

Medium NotDetecta-

ble

Shown Tn7L, 165Tn7R,90-198

5 3(TsnABC)

Ty1 Low NotDone

Shown 4 5 Virus-LikeParticleComplex


Plasmid Rescue, Insert Signal peptides for secreting proteinsTRANSPOSOMICS

Plasmid Rescue, Insert Signal Peptide

☛☛☛☛☛ Inserting an E. coli Origin of Replication toRescue Heterologous Episomes

☛ EZ-Tn5™ <R6Kγγγγγ ori/KAN-2> Insertion Kit

The EZ-Tn5™ <R6Kγ ori/KAN-2> Insertion Kit facilitates sequencing andgenetic analysis of plasmids or any other circularized DNA that would nototherwise replicate in E. coli. The kit uses the EZ-Tn5™ <R6Kγ ori/KAN-2>Transposon which carries the E. coli R6Kγ conditional origin of replica-tion (R6Kγ ori) and a kanamycin resistance marker. A simple, one-step,2-hour in vitro reaction is performed to randomly insert the EZ-Tn5<R6Kγ ori/KAN-2> Transposon into your target DNA. Then an aliquot ofthe reaction is used to transform an E. coli host expressing the pir geneproduct, which is required for replication from the R6Kγ ori. Insertionclones are selected on kanamycin plates and can be sequencedbidirectionally using the provided primers that are homologous to theends of the transposon.

Applications

• “Rescue” plasmids or any other circularized DNA (e.g.,

mitochondria) that would not otherwise replicate in E. coli

because they lack a recognizable origin of replication and/or a selectable marker.

• Prepare DNA sequencing templates from transposon

insertion clones and completely sequence the clone withoutprimer walking or additional subcloning.

• Create a library of random gene knockouts in vitro to

facilitate genetic analysis of plasmid-encoded genes.

#Contents:

EZ::TN™ Transposase, EZ::TN™ <R6Kγ ori /KAN-2> Transposon, EZ::TN™10X Reaction Buffer, EZ::TN™ 10X Stop Solution, Forward and ReversePrimers, Control Target DNA, Sterile Water

☛☛☛☛☛ Kits for Inserting Priming Sites for Sequencing

☛ EZ-Tn5™ <KAN-2>, EZ-Tn5™ <TET-1>, andEZ-Tn5™ <DHFR-1> Insertion Kits

☛ HyperMu™ <KAN-1> and HyperMu™ <CHL-1>Insertion Kits

Please view Page # 55


EZI011RK EZ::TN™ <R6Kγori/KAN-2>Insertion Kit# 10 rxns


R6K0901 EZ::TN™ <R6Kγori/KAN-2> 1 pmoleTransposon


R6KRP091 R6KAN-2 RP-1 Reverse Primer 1 nmole

☛ Finding Sequences that Encode Proteins withSignal Peptides

☛ EZ-Tn5™ βββββ-Lactamase Fusion Kit

The EZ-Tn5™ β-Lactamase Fusion Kit was developed for the direct selectionof genes encoding cell envelope and secreted proteins. The kit featuresthe EZ-Tn5™ <blaM/R6Kγ ori> Transposon which contains a ß-lactamasegene (blaM) that lacks both promoter and secretory signal sequences. Aclone or library of clones is screened with a simple, one-step in vitro

reaction that randomly inserts a single EZ-Tn5™ <blaM/R6Kγ ori>Transposon into the target DNA. E. coli cells are transformed with analiquot of the reaction and plated on media containing ampicillin. Onlyinsertion clones with translational fusions to genes encodingextracytoplasmic proteins will grow. These fusions generate hybridproteins that can transport the blaM moiety through the inner membraneand confer resistance to ampicillin.

Once AmpR clones are selected, use the primer binding sites at the endsof the EZ-Tn5<blaM/R6Kγ ori> Transposon to map or bidirectionally se-quence the insertion site with primers provided in the kit.

**Contents:

EZ::TN™ Transposase, EZ::TN™ <blaM/R6Kγ ori> Transposon, EZ::TN™10X Reaction Buffer, EZ::TN™ 10X Stop SolutionForward and Reverse Primers, Control Target DNA, Sterile Water

☛ EZ::TN™ Transposase

EZ::TN™ Transposase is a hyperactive form of Tn5 transposase. Thehighly purified, single-subunit enzyme can be used to randomly insertany EZ::TN™ Transposon into any target DNA in vitro with an efficiencyup to >106 insertion clones per standard reaction. When incubated withan EZ::TN Transposon in the absence of Mg2+, a stable EZ::TN™Transposome™ complex is formed. The Transposome is so stable that itcan be electroporated into living cells. Once in the cell, the Transposomeis activated by intracellular Mg2+ and the EZ::TN Transposon componentis randomly inserted into the host’s genomic DNA.

☛ HyperMu™ MuA Transposase

HyperMu™ MuA Transposase is a hyperactive enzyme that is at least 50times more active than the MuA transposase available from other suppli-ers of Mu-based sytems. The highly purified, single-subunit HyperMuTransposase can be used to randomly insert any HyperMu™ Transposoninto any target DNA in vitro with an efficiency up to >106 insertion clonesper standard reaction.


TNP92110 EZ::TN™ Transposase(along with reaction and stop buffer) 10 U

THM03210 HyperMu™ MuA Transposase(along with reaction and stop buffer) 10 U


EZI31BL EZ::TN™ β-Lactamase Fusion Kit** 10 rxns


BL0311 EZ::TN™ <blaM/R6Kγori> 1 pmoleTransposon

UFP024 TN FP-1 Forward Primer 1 nmole

BLA032 BLA RP-1 Reverse Primer 1 nmole


Transposon Construction VectorsTRANSPOSOMICS

Custom Transposons

☛ Vectors for Making Custom EZ-Tn5™ Transposons

☛ EZ-Tn5™ Transposon Construction Vectors

EPICENTRE offers four different EZ-Tn5™ pMOD™ Transposon Construc-tion Vectors for the preparation of custom EZ-Tn5™ Transposons (Table1). Each vector contains a multiple cloning site (MCS) flanked by thehyperactive 19-bp Mosaic Ends (ME, denoted by < >) that are specifi-cally and uniquely recognized by EZ-Tn5™ Transposase. To prepare yourtransposon, clone any DNA sequence of interest (e.g., selectable marker,control element, reporter gene) into the MCS and then generate thetransposon either by PCR amplification or restriction enzyme digestion.

The Transposon Construction Vectors pMOD-2<MCS> and pMOD-3<R6Kγ ori/MCS> are pUC-based vectors. They consist of ME sequencesthat flank an MCS in a vector with a colE1 origin of replication (Figure 1).EZ-Tn5 pMOD-3<R6Kγori/MCS> also contains an R6Kγori within the MEsequences, which is useful for a variety of rescue cloning applications.These vectors work well for constructing transposons in most cases.

The colE1 ori was eliminated in pMOD-4<MCS> and pMOD-5<R6Kγori/MCS>, which only have the R6Kγori (Figure 2). Replication from the R6Kγorigin in these two new vectors is dependent on the pir gene productproduced by TransforMAX™ EC100D™ pir+ and pir-116 E.coli cells. Sincemost bacterial strains do not contain a pir gene, the uncut plasmid DNAthat contaminates these transposon preparations can’t replicate andbackground problems are eliminated.

Your custom EZ-Tn5 Transposon can be used for insertion into any targetDNA in vitro. In vitro transposition of R6Kγ ori-containing transposonscan be used, for example, to rescue plasmids that would not otherwisereplicate in E. coli because they lack a recognizable origin of replicationand/or a selectable marker. You can also make your own EZ-Tn5™Transposomes for random insertion into genomic DNA of living cells byincubating your custom EZ-Tn5 Transposon with EZ-Tn5 Transposase inthe absence of Mg2+. The presence of an R6Kγ ori enables you to rapidlyrescue the genomic DNA flanking the transposon insertion site.

Figure 1. EZ-Tn5™ Transposon Construction Vectors pMOD™-2<MCS>and pMOD™-3<R6Kγγγγγori/MCS> replicate in standard E. coli strainsusing a colE1 origin of replication. Transposons made with the

pMOD™-3<R6Kγγγγγ ori/MCS> vector also have an R6Kγγγγγ ori within the

transposon, for rescue cloning applications.

Figure 2. EZ-Tn5™ Transposon Construction Vectors pMOD™-4<MCS>and pMOD™-5<R6Kγγγγγori/MCS> lack a colE1 origin of replication andrequire E. coli strains that produce a pir gene product for replica-tion.


MODFSP201 pMOD™<MCS> ForwardSequencing Primer 1 nmole

MODRSP202 pMOD™<MCS> ReverseSequencing Primer 1 nmole

MODFP931 pMOD™<MCS> Forward PCR Primer 1 nmole

MODRP941 pMOD™<MCS> Reverse PCR Primer 1 nmole


MOD0602 EZ::TN™ pMOD™-2<MCS> 20 µgTransposon Construction Vector

MOD1503 EZ::TN™ pMOD™-3<R6Kγ ori/MCS> 20 µgTransposon Construction Vector

MOD4804 EZ-Tn5™ pMOD™-4<MCS> 20 µgTransposon Construction Vector

MOD4805 EZ-Tn5™ pMOD™-5<R6Kγ ori/MCS> 20 µgTransposon Construction Vector

EZ-Tn5™ TransposonConstruction Vectors

ori that is locatedon vector outside of

the ME sequences

ori that is locatedwithin the ME

sequences

pMOD™-2<MCS>

pMOD™-3<R6Kγori/MCS>

pMOD™-4<MCS>

pMOD™-5<R6Kγ ori/MCS>

colE1

colE1

R6Kγori

None

None

R6Kγ ori

None

R6Kγori

Table 1 : Replication origins in EZ-Tn5Transpson ConstructionVectors.

Transposon Construction vectors include Forward and Reverse Primers


In Vivo Transposomics™: Revolutionizing Microbial GeneticsTRANSPOSOMICS

In-vivo mutagenesis

☛ EZ-Tn5™ Transposome™ Kits

☛ EZ-Tn5™ <R6Kγγγγγ ori/KAN-2>Tnp Transposome™ Kit

Among the advantages of transposon mutagenesis is that the transposonserves as a marker that can be used to clone and sequence the region ofgenomic DNA that has been disrupted. Nothing makes this cloning processeasier than creating mutations in vivo with the EZ-Tn5™ <R6Kγ ori/KAN-2>Tnp Transposome™ Kit. In addition to encoding a kanamycin-resis-tance gene, the transposon contains an E.coli conditional origin of repli-cation (R6Kγ ori). The presence of this origin of replication enables youto propagate or “rescue” the region of genomic DNA into which thetransposon has been inserted.

Figure 1. The process for rescue cloning of transposon insertionsites in genomic DNA using the EZ-Tn5™ <R6Kγγγγγ ori/KAN-2>TnpTransposome™ and TransforMax™ EC100D™ pir+ or TransforMax™EC100D™ pir-116 Electrocompetent E. coli.


TSM08KR EZ::TN™ <R6Kγori/KAN-2>Tnp 10 rxnsTransposome™ Kit*

TNP92110 EZ::TN Transposase 10 U

R6K0901 EZ::TN™ <R6Kγori/KAN-2> 1 pmoleTransposon


R6KRP091 R6KAN-2 RP-1 Reverse Primer 1 nmole

*Includes unlabeled forward and reverse sequencing primers.

☛ EZ-Tn5™ <KAN-2>Tnp and EZ-Tn5™<DHFR-1>Tnp Transposome™ Kits

EZ-Tn5™ Transposome™ complexes are formed between an EZ-Tn5™Transposon and EZ-Tn5™ Transposase and provide a simple and reliablemethod for generating a library of random gene knockouts in vivo. Justelectroporate the EZ-Tn5 Transposome into any of a broad range ofliving cells and select for a marker encoded by the EZ-Tn5 Transposon.Because there is no need for cell conjugation, suicide vectors, or spe-cific host factors, EZ-Tn5 Transposomes are ideal for creating mutantsin species that have poorly described genetic systems or lack adequatemolecular tools.

Ready-to-use EZ-Tn5 Transposomes are available containing either a kana-mycin selectable marker (<KAN-2>) or dihydrofolate reductase gene(<DHFR-1>) that can be selected on plates containing trimethoprim. Thesemarkers are readily expressed in many gram-negative bacteria. Or youcan create your own EZ-Tn5 Transposome using one of the EZ-Tn5™ pMOD™Transposon Construction Vectors and EZ-Tn5 Transposase.

☛ EZ-Tn5™ <KAN-2>Tnp and EZ-Tn5™ <DHFR-1>Tnp Transposome™ Kits (Contd.)

EZ::TN Transposome-mediated insertions have already been usedsuccessfully in a variety of bacteria including Acinetobactor,

Campylobacter, Escherichia, Mycobacterium, Proteus, Pseudomonas,

Saccharomyces, Salmonella, Trypanosoma, Xylella, and others. Thenumber of transposition clones obtained is highly dependent on thetransformation efficiency of the host cell.

*Each kit contains a ready-to-use Transposome™ complex with either akanamycin resistance marker or dihydrofolate reductase gene (forselection with trimethoprim) and forward and reverse primers forsequencing.

☛☛☛☛☛ HyperMu™ Transposome™ Kits

☛ HyperMu™ <R6K•ori/KAN-1>Tnp Transposome™ Kit

The HyperMu <R6K?ori/KAN-1>Tnp Transposome is the stable complexbetween HyperMu™ Transposase and a HyperMu™ Transposon containingan R6K? E. coli conditional origin of replication and a kanamycin select-able marker. The Transposome can be electroporated into living cellswhere it is activated by intracellular Mg2+ and the HyperMu Transposon israndomly inserted into the host’s genomic DNA. Transposon insertionclones are selected on kanamycin plates or by screening for a desiredgene “knockout” or loss of gene function.

The transposon insertion site can be sequenced directly using bacterialgenomic DNA as template and the provided primers that are homologousto the ends of the inserted HyperMu Transposon. Alternatively, the inser-tion site and its flanking DNA can be “rescued” as a plasmid and thensequenced.


TSM99K2 EZ::TN™ <KAN-2>Tnp 10 rxnsTransposome™ Kit*

TSM99D1 EZ::TN™ <DHFR-1>Tnp 10 rxnsTransposome™ Kit*

TNP92110 EZ::TN Transposase 10 U

KAN2001 EZ::TN <KAN-2> Transposon 1 pmole



DFR1001 EZ::TN <DHFR-1> Transposon 1 pmole

DFP9121 DHFR-1 FP-1 Forward Primer 1 nmole

DRP9131 DHFR-1 RP-1 Reverse Primer 1 nmole


MTS32RK HyperMu™ <R6Kγori/KAN-1>Tnp 10 rxnsTransposome™ Kit*

THM03210 HyperMu™ Transposase 10 U

MR6K323 HyperMu™ <R6Kγori/KAN-1> 1 pmoleTransposon

MKFP321 MUKAN-1 FP-1 Forward Primer 1 nmole

MKRP322 MUKAN-1 RP-1 Reverse Primer 1 nmole

**Includes unlabeled forward and reverse sequencing primers.


☛☛☛☛☛ Competent Cells for Replication of R6Kγγγγγori-Containing Insertion Clones

☛ TransforMax™ EC100D™ pir+ and pir-116Electrocompetent E. coli

The TransforMax™ EC100D™ pir+ Electrocompetent E. coli andTransforMax™ EC100D™ pir-116 Electrocompetent E. coli each expressthe π protein (the pir gene product) for replication of plasmids contain-ing the R6Kγ origin of replication (R6Kγ ori).

The TransforMax EC100D pir+ Electrocompetent E. coli will maintainR6Kγ ori-containing plasmids at approximately 15 copies per cell, forcloning of potentially toxic or unstable DNA sequences. The TransforMaxEC100D pir-116 Electrocompetent E. coli are for high copy propagationof up to 250 rescue plasmid copies per cell.

Benefits

• lacZ∆M15 for blue/white screening of recombinants.

• Restriction minus (mcrA, ∆(mrr-hsdRMS-mcrBC)) enables

efficient cloning of methylated DNA.

• Endonuclease minus (endA1) to ensure high yields of DNA.

• Recombination minus (recA1) for greater stability of large

cloned inserts.

Transformation Efficiency

Greater than 1 X 109 colony forming units (cfu)/µg with pR6Kancontrol DNA.


ECP09500 TransforMax™ EC100D™ pir+ 5 x 100 µlElectrocompetent E.coli

EC6P095H TransforMax™ EC100D™ pir-116 5 x 100 µlElectrocompetent E.coli

Includes control vector containing an R6Kγori.

☛ Make Unidirectional Truncations of a ProteinEncoded by Any Sequence

☛☛☛☛☛ EZ-Tn5™ Protein Truncation Kit

☛☛☛☛☛ Mu-End™ Protein Truncation Kit

The EZ-Tn5™ Protein Truncation Kit and the Mu-End™ Protein TruncationKit provide a convenient method for generating a library of N-terminaland C-terminal protein deletions that can be expressed in E. coli. TheEZ-Tn5 Protein Truncation Kit features the EZ-Tn5™ <p15Aori /KAN-2/T7Exp> Transposon, which is randomly inserted into any target DNA usinga simple, in vitro reaction catalyzed by EZ-Tn5™ Transposase. Then, thetransposition reaction is amplified by PCR using one primer that annealsnear a transposon end and another primer that anneals to a fixed pointin the target sequence. Since the transposon is randomly inserted alongthe length of the target sequence, amplification generates a library of N-terminal or C-terminal deletions, depending on the choice of primers.Using the End-Repair Mix and Fast-Link DNA Ligase provided in the kit,the pool of PCR products is blunt-ended and self-ligated to create alibrary of kanamycin-resistant “rescue” clones that can replicate fromthe p15Aori in standard strains of E. coli. Rescue clones with an N-terminal deletion will also contain a transposon-derived T7-promoterregion in a 5'-to-3' orientation. At least one-third of these clones willgenerate in-frame protein fusions that can be expressed in cells contain-ing a T7 RNA polymerase gene (e.g., E. coli BL21).

*Contents:

EZ-Tn5™ TransposaseEZ-Tn5™ <p15Aori /KAN-2 /T7Exp> Transposon, EZ-Tn5™ 10X ReactionBuffer, EZ-Tn5™ 10X Stop Solution, Control Target DNA, Sterile Water,End-Repair Enzyme Mix, End-Repair 10X Buffer, ATP, dNTPs, Fast-Link™ DNA Ligase, PCR Precipitation Solution, and P15 RP-1, P15 FP-1,KAN-2 RP-2, and KAN-2 FP-1 Primers.

**Contents:

HyperMu™ Transposase, Mu-End™ Transposon, HyperMu™ 10X ReactionBuffer, HyperMu™ 10X Stop Solution, Sterile Water, End-RepairEnzyme Mix, End-Repair 10X Buffer, ATP, dNTPs, Fast-Link™ DNALigase, PCR Precipitation Solution, and MU-1 RP-1 primer.


EZI41110 EZ-Tn5™ Protein Truncation Kit * 10 rxns

HMI41110 Mu-End™ Protein Truncation Kit * 10 rxns

Figure 1. The EZ-Tn5™ Protein Truncation Kit can be used tocreate random, unidirectional deletions from the 5'-end (shownhere) or 3'-end of a target sequence

Comp. Cells for R6Kγori Clones/ Protein truncation with transposonsTRANSPOSOMICS

Comp cells/Protein Engg.

☛☛☛☛☛ EZ-Tn5™ Protein Truncation Kit

☛☛☛☛☛ Mu-End™ Protein Truncation Kit (Contd.)


☛☛☛☛☛ EZ::TN™ In-Frame Linker Insertion Kit

The EZ::TN™ In-Frame Linker Insertion Kit is a transposon-based proteinmodification system that was designed to rapidly and easily producerandom 57-basepair (19 amino acid) insertions into a cloned DNA (e.g.genes and cDNA) that encodes an expressed protein.

The kit features the EZ::TN™ <Not I/KAN-3> Transposon, which containsa kanamycin-resistance (KanR) marker flanked by Not I restriction sites.An in vitro enzymatic reaction randomly inserts a single EZ::TN <Not I/KAN-3> Transposon into each clone and produces >106 kanamycin-resistantinsertion clones. Insertion clones for further analysis can be identifiedby gene functional analysis or by mapping or DNA sequencing of thetransposon insertion site. Once clones are chosen, the kanamycin resistancegene is excised from the transposon by Not I digestion. Each Not I-digested clone is then ligated and transformed into high-efficiency E.

coli. The resulting clones each contain a single, random 19-codon insertionthat can be read to express protein in all three reading frames. Thus,the protein retains its original amino acid sequence on both sides of theinsertion site.

Benefits

• Generate a population of >106 insertion clones, each with a

single randomly inserted transposon, to provide completecoverage of the clone.

• More versatile than traditional Linker Scanning Mutagen-

esis—the EZ::TN Transposon insertion is random and notlimited to pre-existing restriction endonuclease sites in thecloned DNA.

• Unique Not I restriction site in the linker insertion facili-

tates mapping and additional gene construction.


EZI04KN EZ::TN™ In-Frame Linker 10 rxnsInsertion Kit ##


KN0801 EZ::TN™ <Not I/KAN-3> 1 pmoleTransposon

NKFP072 Not I/KAN-3 FP-2 Forward 1 nmolePrimer

NKRP062 Not I/KAN-3 RP-2 Reverse Primer 1 nmole

## Contents:

EZ::TN™ Transposase, EZ::TN™ <Not I/KAN-3> Transposon, EZ::TN™10X Reaction Buffer, EZ::TN™ 10X Stop Solution, Forward and ReversePrimers, Control Target DNA, Sterile Water

Two unlabeled primers are provided with the kit to facilitatesequencing or mapping of the EZ::TN Transposon insertion site.

☛ Make Truncations of Protein-Coding SequencesCloned in Deletion Vectors

☛ EZ-Tn5™ Plasmid-Based Deletion Machine

The EZ-Tn5™ Plasmid-Based Deletion Machine is used to generate acomplete population of deletion clones from DNA cloned into one of thespecially constructed plasmid deletion vectors (pPDM™-1 or pPDM™-2)that are supplied with the kit. Random, unidirectional deletions aregenerated via an intramolecular transposition reaction by incubating apPDM plasmid clone with EZ-Tn5™ Transposase in a 2-hour reaction. Asingle reaction produces >105 independent deletion clones for use in avariety of applications including DNA sequencing and protein functional


DPM9401 EZ::TN™ Plasmid-BasedDeletion Machine** 10 rxns

PDM1V941 pPDM™-1 Cloning Plasmid 20 µg

PDM2V942 pPDM™-2 Cloning Plasmid 20 µg


PDMSP01 pPDM™ FP-1 Forward Primer 1 nmole

**Contents:

EZ::TN™ Transposase, pPDM™-1 and pPDM™-2 Cloning PlasmidsEZ::TN™ 10X Reaction Buffer, EZ::TN™ 10X Stop Solution, pPDM™ FP-1Forward Primer, pPDM™ RP-1 Reverse Primer, Control pPDM-1 Clone,Sterile Water

☛ pWEB-TNC™ Deletion Cosmid Transposition Kit

Please view page #

☛ Mutagenesis kits

JBS dNTP-Mutagenesis Kit is based on the incorporation of the mu-tagenic dNTP analogs 8-oxo-dGTP and dPTP into an amplified DNA frag-ment by PCR. The mutagenic dNTPs are eliminated by a second PCR stepin the presence of the four natural dNTPs only, resulting in a rate ofmutagenesis of up to 20%.

JBS Error-Prone Kit introduces mutations in the gene of interest usingmodified PCR reaction conditions. An increased MgCl

2 concentration,

the addition of MnCl2 and the unbalanced ratio of dNTPs induce an in-

creased error-rate of the DNA-polymerase. The rate of mutagenesisachieved by error-prone PCR is in the range of 0.6-2.0%.

JBS DNA-Shuffling Kit is designed to generate gene libraries by randomfragmentation of one gene or a pool of related genes. A controlled frag-mentation of the DNA is followed by a random reassembly in a self-priming PCR reaction. This method allows the recombination of sequencesfrom different, related genes. The overall rate of mutagenesis is approx.0.7%.

Protein Engineering using TransposonsTRANSPOSOMICS

Protein Engg.

• Generate a complete population of random deletion clones

without tedious and timed nuclease digests.

• Generate >105 independent deletion clones in a single, simple

enzymatic reaction.

• The deletion process is highly random to ensure production

of a complete population of deletion clones.

• Rapidly screen deletion clones by agarose gel size analysis.

Contents:

*Polymerase, 10x Mutagenesis buffer, dNTP mix, dPTP, 8-oxo-dGTP,PCR grade water** Polymerase, 10x Reaction buffer, 10x Error-prone solution, dNTPerror-prone mix,PCR grade water*** DNase I, 10x Digestion buffer, DNase stop solution, Polymerase,10x Shuffling buffer, dNTP mix, PCR grade water


PP-101 JBS dNTP-Mutagenesis Kit*, 15 Rxns(Random Mutagenesis by dNTP Analogs)

PP-102 JBS Error-Prone Kit**, (Random 15 RxnsMutagenesis by Error-Prone PCR)

PP-103 JBS DNA-Shuffling Kit***, (Random 15 RxnsMutagenesis by DNA Shuffling)


☛ Kits for Inserting Priming Sites for Sequencing

☛ EZ-Tn5™ <KAN-2>, EZ-Tn5™ <TET-1>, andEZ-Tn5™ <DHFR-1> Insertion Kits

EZ-Tn5™ Insertion Kits are designed to simplify and speed up completesequencing of any cloned DNA >2 Kb, without primer walking or subcloning.Just use a simple, one-step in vitro reaction to randomly insert a singleEZ-Tn5™ Transposon containing a selectable marker – either kanamycinresistance (<KAN>), tetracycline resistance (<TET>), or trimethoprimresistance encoded by the dihydrofolate reductase gene (<DHFR>) - intothe clone. Then, transform E. coli cells with an aliquot of the reactionand select for the marker encoded by the EZ-Tn5 Transposon. Use theprimers provided in the kits to sequence insertion clones bidirectionallyfrom primer binding sites at the ends of the inserted transposon.

Benefits

• Faster—Completely sequence plasmid, cosmid, fosmid, and BACclones 10-times faster than by subcloning or primer walking.

• More Complete Sequence Coverage—Based on the highly ran-dom hyperactive in vitro Tn5 transposition system ensuresthat the primer binding sites are distributed throughout yourclone, even into regions that are difficult to subclone or primerwalk.

• A single reaction generates up to >106 insertion clones.• Use a single set of sequencing primers (provided in the kits) to

completely sequence your clone.

Transposon based DNA SequencingSEQUENCING

Transposon Insertion kits

Figure 1. The process for generating DNA sequencing templateusing an EZ-Tn5™ Insertion Kit. Select inserts on kanamycin,

tetracycline, or trimethoprim.


EZI982K EZ::TN™ <KAN-2> Insertion Kit* 10 rxns

EZI921T EZ::TN™ <TET-1> Insertion Kit* 10 rxns

EZI912D EZ::TN™ <DHFR-1> Insertion Kit* 10 rxns


KAN2001 EZ::TN™ <KAN-2> Transposon 1 pmole



TET92101 EZ::TN™ <TET-1> Transposon 1 pmole

TFP9331 TET-1 FP-1 Forward Primer 1 nmole

TRP9341 TET-1 RP-1 Reverse Primer 1 nmole

DFR1001 EZ::TN™ <DHFR-1> Transposon 1 pmole

DFP9121 DHFR-1 FP-1 Forward Primer 1 nmole

DRP9131 DHFR-1 RP-1 Reverse Primer 1 nmole

*Contents of Each Kit:

EZ::TN™ Transposase, EZ::TN™ Transposon, EZ::TN™ 10X ReactionBuffer EZ::TN™ 10X Stop Solution, Forward and Reverse Primers,Control Target DNA, Sterile Water

☛ HyperMu™ <KAN-1> and HyperMu™ <CHL-1>Insertion Kits

Like the EZ-Tn5™ Transposon Insertion Kits, the new HyperMu™ InsertionKits simplify and speed up complete sequencing of any cloned DNA that istoo large to sequence with a single set of sequencing reactions.EPICENTRE’s Mu-based transposition system uses a unique HyperMu™MuA Transposase that retains the highly random insertion characteris-tics of MuA transposase but is at least 50 times more active in vitro thanthe MuA transposase from other suppliers. The higher activity of HyperMuTransposase results in much higher transposition efficiencies that arecritical for obtaining a sufficient number of transposon insertions tocompletely sequence a clone, especially those with large inserts.


HMI032K HyperMu™ <KAN-1> Insertion Kit** 10 rxns

HMI039C HyperMu™ <CHL-1> Insertion Kit** 10 rxns

MKAN320 HyperMu™ <KAN-1> Transposon 250 ng

MKFP321 MUKAN-1 FP-1 Forward Primer 1 nmole

MKRP322 MUKAN-1 RP-1 Reverse Primer 1 nmole

MCHL390 HyperMu™ <CHL-1> Transposon 250 ng

MCFP391 MUCHL-1 FP-1 Forward Primer 1 nmole

MCRP392 MUCHL-1 RP-1 Reverse Primer 1 nmole

**Contents:HyperMu™ Transposase, HyperMu™ <KAN-1> or <CHL-1> TransposonHyperMu™ MuA 10X Reaction Buffer, HyperMu™ 10X Stop SolutionForward and Reverse Primers, Control Target DNA, Sterile Water


☛ DNA Sequencing Kits for Automated Sequencers

☛ SequiTherm EXCEL™ II DNA Sequencing Kits(for automated sequencers)

These SequiTherm EXCEL™ II DNA Sequencing Kits have been optimizedfor use with the following DNA Sequencers: LI-COR® 4000 and 4200 seriesSequencers, NEN® Global IR DNA Sequencers, ALF™ DNA Sequencers andABI PRISM® DNA Sequencers using dye primer technology. The kits con-tain SequiTherm EXCEL™ II DNA Polymerase, which greatly improvessignal intensities, allowing increased sensitivity. The SequiTherm EXCELII DNA Sequencing Kits incorporate advances that enable sequencing of abroad range of templates including those containing very difficult-to-sequence regions. Both cycle and high-temperature isothermal (non-cycle) sequencing protocols are provided.

Benefits

• Sequence templates containing hairpin structures, regions ofhigh GC or AT content, areas of interstrand reannealing, ornucleotide repeats.

• Kits compatible with LI-COR®, NEN®, and ALF™ DNA sequenc-ers (both dye-primer and internal label) and with ABIPRISM® automated sequencers (dye-primer).

• Greater protocol flexibility—optional isothermal protocol tosequence templates that are recalcitrant to cycle sequenc-ing.

• Termination Mixes contain 7-deaza-dGTP to reduce bandcompression during gel electrophoresis.


For LI-COR® or NEN® Global IR2 Sequencers

SE9101LC SequiTherm EXCEL™ II DNA 100 rxnsSequencing Kit - LC for 25-41 cm gels

For LI-COR® or NEN® Global IR2 Sequencers

SE9202LC SequiTherm EXCEL™ II DNA 100 rxnsSequencing Kit - LC for 66 cm gels

For ALF™ DNA Sequencers or ABI PRISM® Sequencers (dye-primer)

SE8301A SequiTherm EXCEL™ II Long-Read™ 100 rxns DNA Sequencing Kit - ALF

Contents:

SequiTherm EXCEL™ II DNA Polymerase, SequiTherm EXCEL™ II Sequenc-ing Buffer, G, A, T, C Termination Mixes, Control Template, Stop/GelLoading Buffer, Sterile Deionized Water

☛ Primers, Stop/Gel Loading Buffers


SF81015 Stop/Gel Loading Buffer, 1.5 mlBasic Fuchsin and Bromophenol Blue Dyes

SA85025 Stop/Gel Loading Buffer, Basic Fuchsin 2.5 ml

P5713F M13/pUC Primer, Forward 2 µg

P5713R M13/pUC Primer, Reverse 2 µg

☛ SequiTherm EXCEL™ II DNA Sequencing Kit

The SequiTherm EXCEL™ II DNA Sequencing Kit is the kit we recommendfor both cycle sequencing and high-temperature isothermal (non-cycle)sequencing with either labeled primers or internal label incorporationusing 32P, 33P, or 35S radionuclides. The kit incorporates advances thatenable sequencing of a broad range of templates including those that areproblematic for most other DNA polymerases. The kit contains SequiThermEXCEL™ II DNA Polymerase, which improves signal intensities allowingsequencing from low amounts of template.


SEM79020 SequiTherm EXCEL™ II DNA Sequencing Kit 20 rxns



Contents:

SequiTherm EXCEL™ II DNA Polymerase, SequiTherm EXCEL™ IISequencing Buffer, G, A, T, C Termination Mixes, G, A, T, C Long-Read™ Termination Mixes, SequiTherm EXCEL™ II Prelabeling Mix,Control Template Control Primer, Stop/Gel Loading Buffer, T4Polynucleotide Kinase10X T4 Polynucleotide Kinase Buffer, SterileDeionized Water

☛ SequiTherm™ Cycle Sequencing Kit

The SequiTherm™ Cycle Sequencing Kit continues to provide researcherswith a simple, rapid, and reliable method for obtaining high-qualitysequence data from a wide variety of DNA templates. However, theSequiTherm EXCEL™ II DNA Sequencing Kit offers greater flexibility,improved sensitivity, and timesavings compared with this originalSequiTherm Kit.


S20100 SequiTherm™ Cycle Sequencing Kit 100 rxns

Contents:

SequiTherm™ DNA Polymerase, 10X Sequencing BufferG, A, T, C Termination Mixes, Control Template, Control PrimerStop/Gel Loading Buffer, T4 Polynucleotide Kinase10X Polynucleotide Kinase Buffer, Sterile Deionized Water

☛ SequiTherm™ DNA Polymerase

SequiTherm™ Thermostable DNA Polymerase is an enzyme preparationdeveloped and optimized for cycle sequencing. SequiTherm DNA Poly-merase has optimal activity at temperatures above 65oC and is stable upto 95oC. The high reaction temperatures that are possible with SequiThermDNA Polymerase eliminate most problems due to nonspecific priming orthe inability to read through secondary structure.


S3305H SequiTherm™ DNA Polymerase 100 rxns

S3301K SequiTherm™ DNA Polymerase 200 rxns

S6501S Stop/Gel Loading Buffer 1.5 ml(Xylene Cyanol and Bromophenol Blue Dyes)

DNA Sequencing- for Automated and Cycle SequencingSEQUENCING

Kits for automated & manual Seq.

For other common sequencing primers,please view page # 13


Purification of Recombinant ProteinPROTEIN PURIFICATION

Bacterial lysis

☛☛☛☛☛ Bacterial Cell Lysis and Removal of Nucleic Acidsfrom Cell Lysates

☛ EasyLyse™ Bacterial Protein Extraction Solution

The EasyLyse™ Bacterial Protein Extraction Solution is designed for lysingbacterial cells for the isolation of proteins, especially recombinant geneproducts expressed in E. coli, without significant loss of enzymaticactivity. It contains a highly active enzyme for cell lysis and a potentnuclease that reduces extract viscosity by digesting all nucleic acids inthe sample. The EasyLyse Solution is formulated as a homogeneous reagentfor ease of use in high-throughput applications. Just add the Enzyme Mixin Lysis Buffer to the cells for rapid lysis and removal of viscosity.


RP03750 EasyLyse™ Bacterial Protein 500, 1-mlExtraction Solution Purifications

or48, 96-wellMicroplates,100 µl/well

Contents:

Lysis Buffer, Enzyme Mix, MgCl2 Solution

☛ Ready-Lyse™ Lysozyme Solution for ProteinExtraction

Ready-Lyse™ Lysozyme Solution is a non-mammalian, non-avian, recombi-nant lysozyme preparation for the lysis of Gram-negative (such as E.

coli) and Gram-positive (such as Bacillus sp.) bacteria. The specificactivity of Ready-Lyse Lysozyme is 200-fold higher than the specificactivity of egg white lysozyme and, therefore, less is needed in a reac-tion. Also, unlike egg white lysozyme, Ready-Lyse Lysozyme Solution isstable at –20°C, eliminating the need to prepare a fresh solution for eachuse.

Escherichia coli

Salmonella typhimurium

Actinobacillus

pleuropneumoniae

Rhodobacter sphaeroides

Shewanella putrefaciens

Flavobacteria odoratum

Oerskovia xanthinolytica

Bacillus subtilis

Bacteria lysed with Ready-Lyse Lysozyme Solution.

Gram-negative Gram-positive


RP03750 Ready-Lyse™ Lysozyme Solution 2 X 106 U

R1804M Ready-Lyse™ Lysozyme Solution 4 X 106 U

R1810M Ready-Lyse™ Lysozyme Solution 10 X 106 U

☛☛☛☛☛ OmniCleave™ Endonuclease


☛ Kit for Bacterial Lysis and Screening of TotalCellular Protein

☛ ReadyPreps™ Protein Preparation Kit (for totalcellular proteins)

The ReadyPreps™ Protein Preparation Kit is a rapid, gentle, enzymaticmethod for lysing bacterial cells and reducing viscosity for the extrac-tion of proteins, especially recombinant gene products expressed inEscherichia coli.

The kit employs Ready-Lyse™ Lysozyme Solution, a stable non-avian, non-mammalian lysozyme that has a higher specific activity than egg whitelysozyme. The higher specific activity of Ready-Lyse allows the additionof less Ready-Lyse Lysozyme, minimizing the amount of extraneous pro-tein in the preparation. OmniCleave™ Endonuclease reduces the viscosityof cell lysates, facilitating handling and processing. RNase I is also pro-vided for removing cellular RNA when the plasmid and protein are ex-tracted simultaneously. The nucleases are included separately and can beadded as needed.

Applications

• Rapid screening of large numbers of expression clones fordesired protein activity for testing the effects of variousparameters such as culture conditions and inductionprotocols on the yield of active protein.

• Simultaneous isolation of plasmid and expression productfrom the same preparation.

• Preparative scale protein purification.

Reagents sufficient to process 5 g of cells.

Contents:

ReadyPreps™ Lysis Buffer A, ReadyPreps™ Lysis Buffer B, OmniCleave™Endonuclease, RNase I, 1 M MgCl

2, 0.5 M EDTA, and Lysis Test Reagent

☛☛☛☛☛ Kit for Bacterial Lysis and Screening ofPeriplasmic Proteins

☛ PeriPreps™ Periplasting Kit (for periplasmicproteins)

The PeriPreps™ Periplasting Kit enables gentle, efficient solubilizationof the outer membrane of E. coli cells, permitting rapid extraction ofproteins from the periplasmic space. This can result in an enrichment ofcertain proteins compared with preparation by lysis of the entire cell.The PeriPreps Kit combines standard periplast preparation methodsusing lysozyme digestion and osmotic shock with the advantages of Ready-Lyse™ Lysozyme. A spheroplast lysis buffer is also provided for isolationof spheroplast proteins. OmniCleave™ Endonuclease is included for di-gestion of nucleic acids, lowering the viscosity of the sample and improv-ing the preparation of spheroplast proteins.


RP78100 ReadyPreps™ Protein 100 1-ml PrepsPreparation Kit(for total cellular proteins)


PS81100 PeriPreps™ Periplasting Kit 100 Preps(for periplasmic proteins)


Affinity Chromatography-Resins, syringe packed columns, MPLC columnsPROTEIN PURIFICATION

Affinity Chromatography

☛☛☛☛☛ Affinity Chromatography Material

Immobilized nucleotides provide a convenient and rapid one-step puri-fication procedure for a large number of proteins such as kinases,GTPases, chaperones, motor proteins, and others. Jena Bioscience of-fers affinity chromatography material that is tailor-made for purifica-tion (or for related assays) of particular NTP-binding proteins.

• NTPs and dNTPs that are linked to the matrix at various

positions of sugar, base or phosphate moiety of thenucleotide

• different types and lengths of linkers

• types of chromatography material ranging from bulk media

to pre-made

• columns that fit any machine

☛ Different types of chromatography material

Bulk mediabased on Sepharose® 4B

Pre-packed syringe columnsbased on Sepharose® 4B

Pre-packed screening columnsbased on Sepharose® 4B

• maximum amount 0.6 ml

• ready-to-use glass columns

• one each upper and lower frit

• upper and lower end piece

• special 96-well-MTP format column holder on request

Pre-packed MPLC columnsbased on Toyopearl AF-650M

• 40 - 90 µ materials

• 1x Omnifit column kit 10x50 mm

• 1x adjustable end piece

• 2x female-female (1/4" to 6 mm) coupling

• 2 m PTFE tubing (1/16" OD x 0.8 mm ID)

☛ Immobilized Nucleotides

☛☛☛☛☛ Immobilized γγγγγ-Aminophenyl-Nucleotides

γ-Aminophenyl-ATP (C10

-Sephorose®)

(linked to the matrix via a C10

-spacer)

☛☛☛☛☛ Immobilized γγγγγ-Aminooctyl-Nucleotides(linked to the matrix via a C8-spacer)

γγγγγ-Aminooctyl-ATP-Sepharose®γγγγγ-Aminooctyl-GTP-Sepharose®

γγγγγ-Aminooctyl-UTP-Sepharose®γγγγγ-Aminooctyl-CTP-Sepharose®

γγγγγ-Aminooctyl-ITP-Sepharose®γγγγγ-Aminooctyl-XTP-Sepharose®

γγγγγ-Aminooctyl-dATP-Sepharose®γγγγγ-Aminooctyl-dGTP-Sepharose®

γγγγγ-Aminooctyl-dUTP-Sepharose®γγγγγ-Aminooctyl-dCTP-Sepharose®

γγγγγ-Aminooctyl-dTTP-Sepharose®

Immobilized γ-Aminohexyl-Nucleotides(linked to the matrix via a C6-spacer)

γγγγγ-Aminohexyl-ATP-Sepharose®

γγγγγ-Aminohexyl-GTP-Sepharose®γγγγγ-Aminohexyl-UTP-Sepharose®

γγγγγ-Aminohexyl-CTP-Sepharose®γγγγγ-Aminohexyl-ITP-Sepharose®

γγγγγ-Aminohexyl-XTP-Sepharose®γγγγγ-Aminohexyl-dATP-Sepharose®

γγγγγ-Aminohexyl-dGTP-Sepharose®γγγγγ-Aminohexyl-dUTP-Sepharose®

γγγγγ-Aminohexyl-dCTP-Sepharose®γγγγγ-Aminohexyl-dTTP-Sepharose®

Immobilized γγγγγ-Aminophenyl-Nucleotides

(linked to the matrix without a spacer)

Aminophenyl-ATP-Sepharose®(No linker)

Immobilized 8-Aminohexyl-Nucleotides

(linked to the matrix via a C6-spacer)

8-[(6-Amino)hexyl]- amino-ATP-Sepharose®

Immobilized 8-Aminobutyl-Nucleotides

(linked to the matrix via a C4-spacer)

8-[(4-Amino)butyl]- amino-ATP-Sepharose®

Immobilized N6-Aminohexyl-Nucleotides(linked to the matrix via a C

6-spacer)

N6-(6-Amino)hexyl-ATP-Sepharose®

Immobilized N6-Aminobutyl-Nucleotides(linked to the matrix via a C4-spacer)

N6-(4-Amino)butyl- ATP-Sepharose®

Immobilized 2’,3’-EDA-Nucleotides

2’/3’-EDA-ATP-Sepharose®2’/3’-EDA-GTP-Sepharose®2’/3’-EDA-UTP-Sepharose®2’/3’-EDA-CTP-Sepharose®2’/3’-EDA-ITP-Sepharose®2’/3’-EDA-XTP-Sepharose®

Immobilized Tyrosines

Immobilized Tyrosine(linked to the matrix via a C

10-spacer)

O-Phospho-L-Tyrosine-Sepharose®(C

10-linked)

Immobilized Tyrosine(linked to the matrix without a spacer)

O-Phospho-L-Tyrosine-Sepharose®(no linker)


☛ Owl Simple Cast Minigel Systems

Specifications:

Gel Size Combs Included

20cmW x 25cmL 16,24 &36-Tooth (1.5mm thick)

The Simple Cast system’s unique pouring method withstands hot agarose(as high as 60oC without) warping the UVT gel tray. No tape, castingdams, or other accessories are required. All Simple Cast Systems accom-modate two comb positions, allowing the user to run two series of samplessimyltaneoulsy.

☛ Owl Spider Electrophoresis System

The Spider Electrophoresis System al-lows for screening of up to 80 samples ona single agarose gel. Unit features du-rable acrylic construction with platinumelectrodes and gold plated banana plugsto resist corrosion. The EasyCastTM

Gasketed Gel Tray also makes castingeasy with no tape, casting dams, no otheraccessories required.

Complete System Includes:• Buffer Chamber• SuperSafe Lid With Attached Power Cords• EasyCastTM Gasketed UVT Gel Tray• 4 Combs: Two 30-Tooth & Two 40-Tooth, 1.5mm Thick (Other

Combs Available)• Casting Chamber• Gel Size: 14.4cmW x 10.2cmL

Running Buffer Volume: 600ml

Each kit comes complete with 2 open frames, 2 solid back plates, 8 sideclips, and sheets of pre-cut cellophane.

☛ Owl Buffer Puffer Self Recirculating Gel Systems

The Buffer Puffer Self Recirculating Gel Sys-tems feature a built-in buffer recirculationsystem for sharper bands.

Bubbles are collected on the cathode elec-trode and travel up a tube to the anode sideof the gel box. These moving bubbles createeffective recirculation within the chamberwith no external pumps, tubing, stir bars oradditional assembly required. Systems areideal for long runs, multiple sample sets, andRNA gels.

Model Gel Size Combs Included

B1A 7 x 8cm Two Combs: 6-Tooth & 10Tooth(1.5mm Thick)

B1 9 x 11 cm Two Combs: 10-Tooth & 14-Tooth(1.5mm Thick)

B2 12 x 14cm Two Combs: 12-Tooth & 20-Tooth(1.5mm Thick)

The Simple Cast Horizontal Minigel Sys-tems combine a proven, simple, in-placecasting design with new improvementslike lane visualization strips and fluo-rescent rulers. Three gel sizes are avail-able for increased sample capacity andrunning length. The all-in-one design letsyou cast and run gels in the same cham-ber, eliminating the need for additionalequipment of any kind.

☛ Owl Centipede Minigel System

The Liberal Wide Format Minigel Sytem offers a simple, convenient, andfast method for screening multiple samples on a single agarose gel. Up to200 samples can be run simultaneously on one gel; producing clear, tightbanding patterns with no “smiling”. Each side of the gel tray is fittedwith a gasket, which allows the tray to fit snugly into the casting chamberto provide a leak proof seal without tape. Built-in leveling ensures thecasting of flat, uniformly thick gels. Sample loading is greatly enhancedusing microtiter format combs engineered to allow direct loading ofsamples from a 96-well plate using a multichannel pipettor.


D3-14 23cm Four Combs: Four 50-x Tooth (1.5mm Thick)14cm Microtiter Format)

The Liberal Wide Format Minigel Sytem offers a simple, convenient, andfast method for screening multiple samples on a single agarose gel. Up to200 samples can be run simultaneously on one gel; producing clear, tightbanding patterns with no “smiling”. Each side of the gel tray is fittedwith a gasket, which allows the tray to fit snugly into the casting chamberto provide a leak proof seal without tape. Built-in leveling ensures thecasting of flat, uniformly thick gels. Sample loading is greatly enhancedusing microtiter format combs engineered to allow direct loading ofsamples from a 96-well plate using a multichannel pipettor.

☛ Owl WhirlSystem Recirculating Gel System

The WhirlSystem prevents the formation of pH and ionic gradients bythe patented WhirlSystem that allows self-recirculation of buffer with-out the need for a separate recirculator or accessories. Bubbles arecollected on the cathode electrode and travel up a tube to the anode sideof the gel box.

The WhirlSystem is ideal for long runs, multiple sample sets, or RNA gels.It delivers clearer results for samples run over long time periods, andalso eliminates uneven migration, banding distortion, or dissociation ofpH-dependent glyoxicated RNA molecules that can result when ionic deple-tion occurs.


B3 12 cm Two Combs:x 12-Tooth & 20-Tooth14 cm (1.5 mm Thick)

The Really Big Horizontal Gel System isdesigned for detailed RNA/DNA analy-sis. The unit includes the buffer cham-ber, SuperSafe lid with attached powercords, UVT gel tray with gasketedendgates, and 3 combs.


A2 20cm x 25 cm Three Combs:16-Tooth, 24-Tooth &36- Tooth (1.5mm Thick)

☛ Owl Gator Gel System

Submarine Gel ElectrophoresisINSTRUMENTS

Electrophoresis


Submarine & Vertical Electrophoresis SystemsINSTRUMENTS

Electrophoresis

Complete System Includes:• Buffer Chamber• SuperSafe Lid with Attached

Power Cords• UVT Gel Tray with Gasketed End

Gates• 4 Combs: (2) 25-Tooth, (2) 50-

Tooth, 1.5mm Thick• Gel Size: 23cmW x 25cmL• Warranty: 3 years

The Millipede system allows researchers to screen up to 500 samples atone time. The new model A6 wide-format system offers a simple,convenient, and fast method for screening high numbers of samples on asingle agarose gel. Up to 500 samples can be run simultaneously on onegel; producing clear, tight banding patterns with no “smiling”. Sampleloading is greatly enhanced using microtiter format combs. Using thesecombs and a multichannel pipettor, samples may be loaded directly froma 96-well plate.

☛ Owl Millipede Horizontal System

☛ Owl Gator Horizontal Gel System

The Gator Horizontal Gel Systems aremade to withstand heavy workloads andundertake different gel-running require-ments. Comb slots on the UVT tray arepositioned so that you can arrange two,three, or four sample sets to run equallengths on the same gel.


A1 13cm x 25cm Three Combs: 12-Tooth, 16-Tooth,20-Tooth(1.5mm Thick)

A3-1 23cm x 40cm Four Combs: Two 25-Tooth & Two50-Tooth(1.5mm Thick)

A4 20cm x 25cm Two Combs: Two 24-Tooth(1.5mm Thick)

Model A1 has the smallest format of the standard systems. This is apractical unit, which will conserve bench space.

Model A3-1 is an extra long apparatus especially designed for screeninglarge numbers of samples. The gel tray fits 25 and 50-tooth microtiterformat combs for fast sample loading with a multichannel pipettor. Bufferexchange ports are standard on this model.

Model A4 is a lower cost alternative, ideal for teaching labs.

A wide selection of combs is available, including options that can doubleyour sample capacity by doubling the number of sample wells.

Each system is also available with built-in buffer exchange ports thatallow you to circulate buffer for extended runs.

☛ IBI MP1015 Electrophoresis System

The ideal multi-purpose gel box forextended high resolution, linear runs andeconomical quick screens.• Gel Size: 10cmW x 15cmL• Buffer Volume: 500ml• Safety cover and power cords• Casting system and tray chamber• Two 2.0mm thick x 16-tooth combs• Leveling bubble and manual

☛ IBI Ultra-Wide Mini Gel-o-Submarine System

• Gel Size: 23.5cmW x (7.5, 10, or 15)cmL

• Buffer chamber with leveling feetand bubble level

• Safety cover and power cords• QuickCast gel casting system• Two UVT gel trays, 7.5cm and 15cm

long• Two 1.5mm thick 25-well & 50-well

microtiter combs

Designed to perform large numbers of samples, such as PCR or plasmidscreening. Runs 96 samples from a microtiter plate in a single gel inunder 90 minutes.

☛ Owl Puffin Single Sided Minigel System

Cat. No. Description

MTP81 Puffin CompleteSystem, 10 x 10cm

MTP81-X Puffin CompleteSystem, 10 x 10cm,With Cooling Core

• Extended upper buffer chamber for even heat distribution.• Designed to minimize buffer volume - less than 100ml per

run.• Full-length side clamps built-in for convenience.• Spring loaded clamping to a silicone gasket ensures a leak

proof seal.• An optional built-in cooling chamber is available for faster

runs with flat, even banding patterns• Corrosion protected platinum electrodes and gold plated

banana plugs.

☛ Owl Adjustable Vertical Systems

Model ADJ1, ADJ2 and ADJ3 are versatileand easy to use. The height of the upperbuffer chamber can be lowered to run pro-tein gels or raised to run sequencing gels. Asoft silicone gasket ensures a leak proofseal. Black binder clamps or pony clampsare used with these units. Each system in-cludes a removable lower and upper bufferchamber with attached pole rings, basewith attached with pole rings, two heavyduty poles and safety interlocking lids withattached power cords.


MTADJ1 16cmW x 14-4cmL



Plates and spacers available in different sizes to match different heights.


☛ Owl Penguin Dual-Gel Water CooledElectrophoresis Systems

Complete Package Includes: UpperBuffer Chamber with Internal CoolingCore, Lower Buffer Chamber, SuperSafeLid with Attached Power Supply Leads,4 Blank Glass Plates, 2 Notched GlassPlates, 2 Notched Alumina Plates, Block-ing Plate for Single Gel Operation, Combs& Spacer Set (See Above), & Joey GelCasting System.

Complete System Includes: All of thecomponents listed above except the JoeyGel Casting System.

Vertical Electrophoresis Systems/Power SuppliesINSTRUMENTS

Electrophoresis

Model Gel Size Buffer Combs/SpacersVolume Included*

P8DS 10cmW x 250ml Two 15-Tooth,8-10 cmL 0.8mmThick &

0.8mm Spacer Set

P9DS 16cmW x 650ml Two 10-Tooth & Two14-16 cmL 15-Tooth, 1.5mm Thick

& 1.5mm Spacer Set

P10DS 20cmW x 1250ml Two 15-Tooth & Two20 cmL 20-Tooth, 1.5mm Thick &

1.5mm Spacer Set

The Power Station Power Supplies are idealfor DNA, RNA, and protein electrophoresisas well as blotting applications. All modelsfeature a microprocessor that utilizes feed-back loops to maintain tight control overoperating parameters. A number of safetyfeatures are incorporated into the softwareincluding automatic crossover, no-load de-tection, sudden load change, and over volt-age protection. Operation of the power sup-plies can be timed or continuous and theoutput can be set to either constant voltageor constant current.

In the event of a power failure, the unit can be programmed to resumethe run with the original settings upon restoration of power. An errorcode will be shown in the display to alert the user that a power failure hasoccurred. All operating parameters are set using the membrane keypadand displayed digitally. Four sets of output terminals allow for the simul-taneous running of up to 4 gels from the same power supply.

☛ Labnet Power Station Power Supplies

Model 200 300 300 Plus

Voltage /Increments 5-200V / 1V 10-300V / 1V 10-300V / 1V

Current /Increments .01-2A / .01A 4-400mA / 1mA 4-500mA / 1mA

Timer 0 to 999min 0 to 999min 0 to 999min

Display 3 Digit LED 3 Digit LED 3 Digit LED

Outputs Terminals 4, Parallel 4, Parallel 4, Parallel

These compact power supplies cover awide range of requirements for electro-phoresis separations. The SH-500 is idealfor DNA or RNA submarine gels, PAGE &SDS-PAGE, multiple horizontal gels andmini blotting applications. It features abuilt-in timer, constant voltage or constantcurrent, and a unique Gel Saver mode (1mAoutput to prevent diffusion after the timerexpires). The easy-to-use SH-3000 offersconstant voltage, current and power, mak-ing it perfect for DNA sequencing systemsand SDS-PAGE applications.

☛ IBI SH-500 & SH-3000 Power Supplies

Model: SH-500 SH-3000

Voltage: 1-500V, 1V steps 10-3000V, 10Vsteps

Max. Current: 300mA, 1mA steps 300mA, 1mA steps

Power: 0.3-150 watts 300 watts

Fault Detection: Stop/Audible Alarm Stop/Audiblealarm

Operating Temperature: 0-40°C 0-40°C

Dimensions (W x D x H): 17 x 24 x 7cm 27 x 28 x 11cm

Weight: 1.6kg 4kg

☛ Owl Lightning Volt Power Supplies

OSP-105

• Upto 250V & 500mA• Constant Voltage• Ideal for DNA/RNA separations

OSP-300/OSP-500

• Upto 300/500V & 500mA• Constant Voltage & constant

current• Ideal for DNA/RNA separations• 4 Output terminals• 7-segment LED display• Built-in timer• Load sensing circuit

These mini-format systems aredesigned to complete analysis 2to 3 times faster than conven-tional gels and are ideal for run-ning all types of precast gels. TheEasy-Snap capturing systemmakes the placement of gelsquick and easy. Both systemsfeature the patented IBI low-fogvented lid that reduces heatbuild-up.

Model: IB90000 IB91000

Gel Size: 8 x 10cm 10 x 10cmInner Buffer Tank Capacity: 125ml 150mlOuter Buffer Tank Capacity: 500ml 350mlVoltage Limit: 6,000 DC 6,000 DCDimensions (W x D x H): 17 x 17 x 18cm 17 x 17 x 18cmWeight: 2.2kg 2.2kg

☛ IBI Universal Protein Systems


Sequencing Gels / ElectroblottersINSTRUMENTS

Electrophoresis / Blotting

☛ Owl Otter Sequencing Gel Casting System

Cat. No. Product

MTSGC-1 Otter System forgels up to 42cmW x48cmL

MTSGC-2 Otter System forgels up to 42cmW x65.5cmL

The Otter uses an innovative method for casting in less than 3 minutes.Relying on a horizontal sliding plate procedure, capillary action drawsgel between plates, and surface tension, not tape, keeps the gels fromleaking. The system is fully adjustable to accomodate glass plates from20cm to 42 cm wide. To cast a gel, simply pour acrylamide and slide topplate into position. Because the gel is poured horizontally, there is almostno mess; decreasing exposure to hazardous acrylamide.

☛ Owl T-Rex Aluminum Backed Sequencing Systems

S3S and S4S Systems Include:

• Set of glass plates two blank, twonotched

• Adhesive temperature indicator• Top and bottom interlock safety

lid with attached power cords• 2 Sharktooth combs and spacer set

(0.4mm thick)• 2 Well Combs


MTS3S Complete SequencingSystem, 35cmW x 45cmL

MTS4S Complete SequencingSystem, 20cmW x 45cmL

Models S3S and S4S feature an anodized, floating aluminum plate heatsink that contacts the gel cassette and aids heat dissipation across thegel. Clear back on the upper buffer chamber allows easy visualization ofwells. The upper buffer chamber is equipped with in-line stopcock drain-age port. The lower buffer chamber is removable for easy cleaning.Acrylic screw clamps are mounted on the unit for easy assembly. Includesbuilt-in leveling.

☛ IBI STS-45I Sequencer

Complete System Includes:

• Main assembly unit

• Aluminum thermoplate

• Two glass plates

• 0.4mm thick comb and spacer set

• One set of power cords

Removable Lower Buffer Tank:Allows the user to dispose of bufferwithout moving the unit.

Interlocking Safety Lids: Both lowerand upper chambers feature ahinged, interlocking safety lid.

Patented Slant-Back Design: With the slant-back design, the IBI STS-45i sequencer assembles with ease. The design ensures that the glassplates remain in place during assembly.

Built-In Aluminum Thermoplate: Distributes heat evenly, eliminating“smiles or frowns”. The unique epoxy-coated white background of thethermoplate makes for easy viewing of dye migration.

96-Well Capacity:The 96-well capacity accommodates 24 templates on asingle gel, resolving up to 6,000 bases (6kb) of sequencing data per run.

Easy-Turn Knobs: The easy-turn knobs create that perfect seal betweenthe gel and the glass plate. These knobs prevent leaking andovertightening.

☛☛☛☛☛ IBI Base Runner 100 & 200 Sequencers

System Components: Main unitassembly, upper & lower bufferreservoirs, one 0.4mm comb andspacer set (includes two 32-wellconventional combs, one 32-wellsharkstooth comb, one 48-toothsharkstooth comb, and four sidespacers), one set of power cords,instruction manual, and one (BaseRunner 100) or two (Base Runner 200)60cm thermocore plates.

Gel Sizes: 27.3 x 19cm /41.2 x 19cm / 56.5 x 19cm

Plate Sizes: 30 x 21.6cm /45 x 21.6cm / 60 x 21.6cm

Upper Buffer Tank Capacity: 500ml

Lower Buffer Tank Capacity: 500ml

Dimensions (W x D x H): 30 x 30 x 75cm

The unique, patented thermocore plates eliminate gel artifacts and mini-mize pre-run time. The glass-aluminum-glass laminate design distributesheat rapidly and efficiently, producing even sample migration for error-free data interpretation.

The buffer tank and tank drain assemblies are removable to permit safeand convenient disposal of radioactive and nonradioactive buffers, whileinterlocking safety covers on both the upper and lower tanks protectagainst electrical hazards. The Base Runner has the flexibility to accom-modate 60, 45, or 30cm gels thanks to its unique adjustable height assem-bly, which is slanted to enhance gel visibility while holding the plates inplace prior to positioning the buffer tanks.

☛ Owl Panther Semi-Dry Electroblotters


• Base with built-in stainlesssteel cathode plate

• Lid with built-in platinum/titanium anode

• Interlocking power cords• 3 Heavy duty knobs (HEP1)• 4 Heavy duty knobs (HEP-3)

The Panther offers rapid transfer of proteins or nucleic acid moleculesfrom polyacrylamide or agarose gels to membranes. Traditional South-ern, northern and western blotting methods can take from several hoursto overnight; using the Panther you can complete the entire procedure inas little as one hour.

Accurate and Efficient - Plate electrodes ensure complete, even trans-fer. Solid plate platinum/titanium and stainless steel electrodes are highlyconductive and allow transfer at low voltages without external coolingsystems. Plate electrodes also provide a uniform electric field for effi-cient, even transfers. Systems are graphite-free to avoid the decompo-sition associated with graphite electrodes.

Two Panther semi-dry electroblotter systems are available. The HEP-1system accommodates mini to full size gels with a transfer area of 20 x20cm, designed specifically for the transfer from polyacrylamide oragarose gels. The HEP-3, with a transfer area of 35 x 45cm, is designedspecifically for the transfer of nucleic acids from sequencing gels tonylon membranes.


☛☛☛☛☛ Owl Bandit Tank Electroblotting System


• Blotting chamber with solid plateelectrodes

• Safety interlocking lid withattached power cords

• Cooling modules• 2 Blotting cassettes• Blotting filter paper, 25 sheets,

18 x 20cm• Transfer Area: 20cmW x 18.5cmL• Buffer Volume: 3L

The Bandit is designed to provide uniform, reproducible protein trans-fer over a wide molecular weight range. Solid plate electrodes consistingof platinum coated titanium anode and a stainless steel cathode providethe high current densities required for efficient and complete transfer.Novel use of alumina ceramic plate as part of its cooling module allows thesystem to effectively maintain low buffer temperatures during transfer.

☛ Owl Bandit MiniTank Electroblotter


• Transfer Area: 9cmW x 9cmL• Blotting Chamber With

Platinum Wire Electrode Panels• Safety Interlocking Lid With

Attached Power Supply Leads• 4 Color-Coded Blotting

Cassettes With Foam Pads• Blotting Filter Paper (16

Sheets, 9cmW x 9cmL)

The Bandit MiniTank Electroblotter is designed to provide uniform,reproducible protein transfer over a wide molecular weight range withrapid, efficient transfers of up to 4 polyacrylamide mini protein gelssimultaneously. Four color-coded cassettes are included with each systemfor easy setup. Cold water is recirculated through the cooling base of theunit, transferring heat from the buffer to keep it cool. The electrodesare deeply seated to prevent damage and are corrosion resistant.

☛ S&S Minifold I Spot, Dot, and Slot-Blot ArraySystems

Widely used for nucleic acid blots, hybridoma screening, TCA precipita-tions, and particle entrapment, the Minifold I system consists of fourbasic components: sample well plate, filter support plate, vacuum ple-num, and metal clamping plate. The sample well plate is available in threeconfigurations for producing spots, dots, or slots. All three plates areinterchangeable and can be purchased as an accessory plate in conjunc-tion with a complete system. Avialable in acrylic and Delrin.

96 Well Dot-Blot Plate (500µl/well): Filter Area: 12.5mm2/dot

96 Well Spot-Blot Plate (200µl/well): Filter Area: 2mm2/spot (1 x 2mm)

48 Well Slot-Blot Plate (1,000µl/well): Filter Area: 6.24mm2/slot (7.8 x0.8mm)

☛ S&S Minifold II 72 Well Slot-Blot Manifold

The Minifold II is a 72 well slot-blot manifold that is designed for precisequantitative solid phase assays and accepts all types of transfer mem-branes. The smaller slot dimensions require lesser samples than dot-blotters and the smaller slot surface area results in higher signal intensi-ties, allowing visualization of results in less time.

Filter Area: 6.0mm2/slot (8.0 x 0.75mm)

Max. Capacity: 600 µl/well, 72 wells

Pressure: 0.9 bar, vacuum

☛ S&S Westran Clear Signal & Westran S PVDFMembranes

Westran S is a 0.2mm hydrophobic PVDF membrane that gives superiorprotein binding capacity (200mg/cm2) and is designed specifically forsequencing applications. Offers the chemical resistance needed for N-terminal sequencing and provides high protein retention even after harshwash steps. The smaller pore size allows for better retention of lowmolecular weight proteins. Westran S can also be used for non-sequenc-ing Western blotting applications.

☛ S&S Gel Blot Papers

Used for Wicking & Gel Support, Membrane & Gel support, Blotting.

Grade Thickness (Typical Values) Surface Absorbency

GB002 Medium, 0.4mm SmoothMedium

GB003 Thick, 0.8mm SmoothMedium

GB004 Extra Thick, 1.2mm Rough Extra High

☛ S&S Protran Nitrocellulose Membranes

S&S Protran membranes are manufactured using 100% pure nitrocellu-lose to ensure the highest binding capacity possible. In addition to highbinding capacity, Protran nitrocellulose membranes inherently have verylow background. The superior surface properties of the membrane guar-antee superior signal-to-noise ratios, without the need for stringentwashing conditions. And unlike PVDF membranes, Protran nitrocelluloserequires no methanol pre-wetting step. This makes it the membrane ofchoice for proteins that prefer aqueous environments. Prior to trans-fer, the membrane is simply wet in water and then in the transferbuffer.

(1) Corners are notched for use with the Minifold I Dot-Blot System.

(2) Cut to fit the Miniford II Slot-Blot System.

Available in sheets, circles, rolls and microplate formats ofdifferent sizes and thickness (0.2 µ, 0.45 µ)

☛ S&S Optitran Supported Nitrocellulose Membranes

Optitran supported membranes are nitrocellulose membranes with apolymer backing that provides exceptional strength and mechanical prop-erties. They provide very low non-specific binding, without stringentwashing and blocking conditions, using standard nitrocellulose protocols.Optitran membranes withstand repeated stripping and re-probing whileproviding excellent signal-to-noise ratio.

(1) Corners are notched for use with the Minifold I Dot-Blot System.(2) Cut to fit the Miniford II Slot-Blot System.

Available in sheets, circles, rolls and microplate formats ofdifferent sizes and thickness (0.2 m, 0.45 m)

☛ S & S Supercharge & Nytran N Nylon Membranes

• Highest possible charge available

• Extremely low background binding.

• Consistent pore size & pore distribution

• Can be used with protocols standardized with earlier version nylonmembranes

Electroblotters, Nitrocellulose, PVDF membranes, Blot papersINSTRUMENTS

Electro blotting


☛☛☛☛☛ Personal Benchtop Centrifuge

☛☛☛☛☛ MTI Mini Microcentrifuge I

For quick spin-downs from wall orcaps of centrifuge tubes, micro-filtration of HPLC samples, and cellseparations. Angle rotor accommo-dates six 1.5/2.0ml tubes or sixUltra Spin Filter Systems. Adapt-ers are available to hold 0.4 and0.5 ml tubes. Starts and stops inseconds by opening and closing lid.

There is an additional rotor whichis designed to accommodate two 8x 0.2ml strip tubes. Perfect forstrip tube PCR applications.

Max. speed: 6000 rpm, Max. RCF:2000 x g

The MTI6000 Mini Centrifuge isideal for quick spin downs andmicrofiltration. Each unit comessupplied with a 6 x 1.5/2.0ml rotorwith adapters for 0.5ml and 0.4mltubes, and an interchangeable sec-ond rotor that will hold 28 x 0.2mlPCR strip tubes. Features an on/off switch and a fixed maximumspeed of 6,000rpm (2,000 x g).

MTI Mini Microcentrifuge II

Model 8454 is a variable speedmicrocentrifuge with a maximumspeed of 10,000 rpm at 5,600 x g.The space-saving design with Lexanpolycarbonate cabinet makes itlightweight and easily moved fromworkstation to cold room, to re-frigerator. Features microproces-sor controlled 10 minute timer withaudible time-up signal, momentaryspin button, and automatic shut-off when lid is opened.

Specifications Includes:

- 6-Place Rotor for 1.5ml & 2.0mlTubes

- 12-Place Rotor for 0.5ml Tubes

- 6 Adapters for 0.4ml Tubes

☛ Variable Speed Microcentrifuge

☛ Spectrafuge 7M Microcentrifuge

Rotor Capacity 12 x 1.5ml/2.0mlTubesMax. Speed 10,000rpm,Max. RCF 7,176 x gTimer 60 minutes or

continuous

Brushless motor: No maintenance, fast acceleration & deceleration.

Cool Operation: Unique ducting system supplies continuous airflow torotor chamber.

Precise control: Variable speeds across broad range, suitable forapplications such as microfiltration, separation of proteins andNucleic acids.

Complete package: Supplied with 18-place angle rotor formicrotubes. Rotor accepts microtubes with screw caps and skirtedbottoms also. Adapters available for smaller tubes.

☛ Spectrafuge 24D Digital Microcentrifuge

The new Spectrafuge 24D Digital Microcentrifuge combines innovationssuch as a unique, easy access rotor, an exclusive multi-flow air-coolingsystem and high performance drive with a digital microprocessor thatprecisely regulates all aspects of operation.

Two “smart” knobs are used to control all operating parameters withspeed and time

values shown digitally on two large displays. Switching from rpm to g-force is accomplished

by simply pressing the speed control knob. Run times from 30 seconds to30 minutes (or continuous) are set with the timer knob. The powerful,brushless motor in the Spectrafuge 24D quickly and effortlessly acceler-ates the rotor to set speed. A computer designed isolation system allowsfor vibration free operation, even with a slight imbalance. A detectorshuts down the centrifuge in the case of a significant imbalance.

A specially designed air cooling system passes ambient air over both thetop and bottom of the rotor as well as through the motor chamber of theSpectrafuge 24D. For samples that require below ambient tempera-tures, the Spectrafuge 24D may be used in a cold room.The uniquedesign of the 24-place rotor allows easy access to the tops of the sampletubes. An optional StripSpin adapter snaps on top of the rotor for spin-ning 0.2ml tubes and strips.

Specifications:

Rotor Capacity 24 x 1.5/2.0ml Tubes

Max. Speed 13,300rpm,

Max. RCF 16,300 x g

Timer 30 minutes, continuous, or momentary operation

The lower speed range of the Spectrafuge 7M Microcentrifuge makes itideal for a variety of applications including quick spin downs, processingmicrofiltration devices, and basic cell separations. The fixed angle ro-tor accommodates 12 x 1.5/2.0ml tubes or smaller tubes through the useof adapters (comes supplied with 12 x 0.5ml/0.6ml adapters). For appli-cations requiring below ambient temperatures, the unit may be used ina cold room.

☛ Spectrafuge

Specifications:

Maximum Speed14,000rpm,Maximum RCF: 16,000 x gAcceleration toMax. Speed: 10sDeceleration from Max. Speed: 9sMax. Radius for1.5/2.0ml:7.3cmTimer: 1-30 min. or holdNoise Level: 55 dB(A)

Mini CentrifugesINSTRUMENTS

Centrifuges


☛ LD Series Dewars

Liquid Nitrogen Dewars & Storage DevicesINSTRUMENTS

Cryogenics

The LD Series ofcryogenic dewars aredesigned for storing anddispensing small amountsof liquid nitrogen. Theseries includes a beakerstyle dewar with a widemouth (LD5) andpitcher-style model foreasy pouring (LD4)

Available in 4L, 5L, 10L, 25L, 35L and 50L capacity.Optional equipment includes Cap, Necktube core, Roller base, Tippingstand and liquid withdrawal device.

Features:

• Modern construction and advanced insulation materials assure highthermal efficiency

• Ribbed high strength aluminum body, magniformed necktubedesign, low VOC

• Easy Operation - Snap-On cap and necktube assures tight closureand easy access

• Superior vacuum performance with super insulation providesmaximum holding times.

The Extended TimeSeries (XT) of cryogenicrefrigerators isdesigned for long-termstorage of a variety ofmaterials at cryogenictemperatures.This series also offers alow profile model (XTL)for use in more confinedworkspaces.

☛ Taylor-Wharton XT Series Cryogenic Refrigerators

Available in 3L, 8L, 10L, 20L, 34L capacity.Optional equipment includes Canisters, Necktube core, Roller base andCryo-Sentry alarm.

Features

• Ribbed high strength aluminum body, magniformed necktube design• Versatile Storage System - convenient canister index location ring

and internal spider super vacuum performance• Superior vacuum performance with super insulation provides

maximum holding times• Security - Accessory low-level alarm is available for more valuable

samples

☛ HC Series Refrigerators

The high capacity (HC) seriesof refrigerators are designedfor storing large quantities ofa variety of materials atcryogenic temperatures.Temperatures generally rangebetween -320º F (-196º C) atthe liquid surface, and -299º F(-184º C) under the closednecktube core.

☛☛☛☛☛ HC Series Refrigerators

• Large storage capacity• New rugged construction - magniformed necktube design, ribbed

high strength aluminum body• Versatile storage system - convenient canister index location ring

and internal spider.• Superior vacuum performance with super insulation provides

maximum holding times.• Security - Optional low-level alarm is available for use with more

valuable samples

Available in 12L, 20L, 34L and 35L capacity.Optional equipment includes Canisters, Necktube core, Roller base,Cryo sentry level alarm.

☛☛☛☛☛ LS Series Refrigerators

The Taylor-WhartonLaboratory Systems (LS)are uniquely designed forlarge vial capacity inconvenient box-typestorage racks. Theyprovide maximum holdingtimes. This means loweroperating costs per vialand fewer refills.

Features• LS6000 available with AutoTend Controller• New Rugged Construction - ribbed high strength aluminum body,

magniformed necktube design, and more durable paint• Convenient Storage Systems - rack index location ring and internal

location spider, computer compatible box storage is ideal forsimple inventory management

• Super vacuum performance with super insulation providesmaximum holding times

• Security - Accessory low-level alarm is available for more valuablesamples

• Mobility - Roller bases are available

Safe and easy to use for the storage of liquid nitrogen and other lowtemperature liquid gases.Dewars are constructed of unbreakable stainless steel and areresistant to vibration, impact or shock.Dewars are supplied with lid and detachable carrying handle.

☛ Unbreakable Stainless Steel Liquid NitrogenDewars


Benchtop Freezers, Chillers, Freezer Storage Boxes & racksINSTRUMENTS

Cryogenics

☛☛☛☛☛ Polar Block II -15ºC

☛☛☛☛☛ Personal Benchtop Freezer

Specifications

Dimensions: 9.75”D x 11.75”W x7.25”HNet Weight: 10 lbs.Electrical: 220 VAC, 100 WTemperature Range: -15°C (Typically)Performance: 35°C to 39°C BelowAmbientWarranty: Two Years Parts & Labor

The Polar Block II is ideal for intermediate term storage of restrictionenzymes and other labile reagents for applications requiring sub-freez-ing temperatures, such as ethanol precipitation. The Polar Block II usesa solid state, air-cooled thermoelectric device. It is equipped with adigital LCD temperature display and will reach and maintain tempera-tures from 35°C to 39°C below ambient (typically -15°C) in approxi-mately 30 minutes. Each unit comes with a removable machined aluminumblock that accommodates 14 x 1.5ml microcentrifuge tubes. Additionalblocks and block configurations are also available.

☛☛☛☛☛ MiniFridge II+4ºC to Ambient

The Minifridge II Cold BlockIncubator is the convenient andreliable way to incubate or storesamples at temperatures from +4°Cto ambient. The dry cold of theMinifridge II makes it the idealsubstitute for messy ice buckets.The large LCD display and themicroprocessor controller ensureseasy and accurate temperaturesetting.

The solid-state thermoelectric cooling module is compact andefficient. A large open well accommodates up to two standard dryblocks* (2” x 3” x 3.75”) commonly used in dry block incubators.The unit can also accommodate flasks and beakers and can function asa small refrigerated water bath.

• Blocks not included•

Specifications Dimensions: 9.75”D x 11.75” W x 7.25” HWeight: 10 lbs.Electrical: 220VAC, 100WTemperature Range: +4°C to AmbientPerformance: Typically 0.5° From Set PointWarranty: Two Years Parts & Labor

☛ IsoFreeze Flipper Temperature Maintenance Racks

Available in two temperature ranges,these racks will provide consistentthermal protection for extendedperiods. Made of virtually unbreak-able polycarbonate, both units arefilled with a non-toxic gel, arestackable, and will accommodate upto 20 x 1.5/2.0ml micro or screwcap tubes, or 20 x 0.5ml tubes on thereverse, flip side. Available in , 0°C& -20°C

Replace your disposable cardboard racks with these sturdy, reusablepolypropylene storage racks. Accommodates 0.5, 1.5, and 2.0ml tubesand withstand temperatures as low as -90°C. Will easily fit standardfreezer storage racks and drawers. An alpha-numeric grid is hot stampedon the unit in clear, easy to read contrasting colors. Bases are availablein natural, black, or assorted fluorescent colors.

☛☛☛☛☛ 81 Well Freezer Storage Boxes

• Accommodates 0.5, 1.5, and 2.0mltubes.

• Autoclavable up to 15 mins.• Freezer safe to -90°C.• Fits all standard freezer inventory

systems.• Fitted lids included.• Contructed from polypropylene

copolymer.

Both racks are alpha-numeric matrixed, hotstamped on the lid incontrasting color for fast, easy, and error-free identification ofindividual tube samples.

These sturdy, reusablepolypropylene racks include ahinged lid and are rated to -80°C. Racks will accommodate1.5/2.0ml microtubes andstandard 2.0ml cryo vials. Thelids have raised edges to allowsafe, non-slip storage ofmultiple racks.

☛☛☛☛☛ 100 and 50 Well Freezer Storage Boxes

☛☛☛☛☛ ArcticChill Portable Cooler

Keeps samples cold for eighthours or longer. Small enoughto fit in the palm of yourhand, yet holds 12 x 1.5/2.0ml tubes! Patentedrefrigerant in sides, bottom,and lid surrounds samples incold.Ideal for enzyme storage andtransporting samples.

☛ 64 Well Freezer Storage Boxes


☛ PRO200 Hand-Held or Post-Mounted LaboratoryHomogenizer

Homogenizers Hand-held and Benchtop, BeadbeatersINSTRUMENTS

Homogenizing & Tissue Disintegration

The Industry’s Most Versatile & AffordableLightweight/High-Torque Homogenizer.

The PRO200 is fast and quiet, with mosthomogenizations taking just seconds at amaximum 72db at full speed. The PRO200features a 125-watt, high-torque, variablespeed motor and can be used with a vari-ety of different PRO QUICK CONNECT ro-tor stator generators. The PRO200 can beused as a hand-held unit or can bepostmounted, and will homogenize sampleswith volumes from 0.03ml to 2L.Variable Speed: 5,000 to 30,000rpmPower: 125 WattsTreatable Volume: 0.03ml to 2L

☛ PRO QUICK CONNECT Rotor Stator Generators

They are manufactured from a high quality 300 Series stainless steel andcan be completely sterilized by autoclaving, hot air, flaming or chemicalprocesses. All generators offer the unique PRO QUICK CONNECT feature,which allows you to change generators with an easy quarter turn. Thegenerators are also designed to help reduce the amount of air intro-duced into the sample during homogenizing to minimize foaming.

Stainless steel sealed chamber assemblies are available for working withhazardous samples. Please call for more information.

Flat Bottom Generators, Saw Tooth bottom Generators, Open SlottedGenerators and Cryogenic Generators available in different sizes.

☛ Multi-Gen 7

The Multi-Gen 7 is a pack of 24 stainless steel generators for use with thePRO 200. Multi-Gen 7 features:• 316 stainless steel and Teflon construction for homogenizing tough

tissue samples and for repeated autoclaving• Eliminates the need for generator cleaning between samples• Hands-free ejection of generator• Ideal for molecular applications, PCR, and microtube assays

☛ PRO250 Hand-Held or Post-Mounted LaboratoryHomogenizer

The PRO250 is a perfect fit between thePRO200 and the larger benchtop PRO ho-mogenizers. It’s light enough to be hand heldfor convenience, yet it has extra torque forprocessing difficult samples. Combining thepower of a 3/4 hp, 576 watt motor with asmooth variable speed control (10,000 to30,000 rpm), the PRO250 can handle samplesfrom 0.05ml to several liters. Like all PROhomogenizers, the PRO250 accepts PROQUICK CONNECT rotor stator generators forfast and virtually effortless changeover.

PRO250 Series Specifications

Power: 576 Watts; Electrical: 220VSingle Handed OperationStand Assembly with Easy-Lift mechanismVariable Speed: 10,000 to 50,000 rpmTreatable Volume: 0.05ml to Several LitersSample Size: 0.02g to 100g

The Tissue-Tearor is a rotor-stator typetissue homogenizer, which can rapidly ho-mogenize, disperse and emulsify samplesin 0.5-50ml of liquid. During operation,the suspended material is drawn into thecore of the homogenizer by a rotor turn-ing at 30,000rpm. The material is repeat-edly cycled through six narrow slits in thestator where it is rapidly sheared and dis-integrated by high frequency mechanicalaction.

Complete homogenization of tissues(muscle, liver, breast tissue, etc.) is usu-ally achieved in ten to thirty seconds.

☛ Tissue Tearor Mixer & Homogenizer

The Mini-BeadBeater is a micro-sized cellhomogenizer. A sealed chamber contain-ing minute glass beads and 1 to 1.5ml oftissue or cell suspension is agitated vio-lently for 1-3 minutes to achieve com-plete cell disruption. Even unusually toughsamples such as bone or microbial sporesare efficiently crushed by the collidingglass beads. The non-foaming method canbe used to isolate delicate enzymes andorganelles or to extract nucleic acids.Totally sealed, it is optimized for eukary-otic or bacterial disruption

Vials: Polypropylene with O-ring seals and cap. Hold about 1ml of lead-free glass beads and 0.1 to 1.0ml of material to be disrupted. Vial snapsinto spring-loaded holder.

Motor: 0.62 Amp with 3 wire grounded cord. Zero to five minute auto-matically resetting timer.

☛☛☛☛☛ PRO300 Benchtop Laboratory Homogenizer

Easily Handles All Common LaboratoryHomogenizing Tasks

The PRO300A & PRO300D feature ahigh-torque, variable speed motorthat operates from 0 to 28,000rpmwith a speed variation of just 1% overthe entire speed range, regardlessof changes in sample viscosity. ThePRO300A features a 3/4 hp, 700-wattmotor and the most advanced closed-loop speed control available. ThePRO300D offers the same advanceddesign as the PRO300A, but also hasan LED display of generator or bladespeed for optimum precision, accu-racy and repeatability.

Variable Speed: 0 to 28,000rpmPower: 700 WattsTreatable Volume: 0.03ml to Several LitersElectrical: 220V

☛ Mini-Bead Beater


Vortex Mixers and StirrersINSTRUMENTS

Mixing & Stirring

The Bio-Pulverizer re-duces tissue hard-frozenin liquid nitrogen to afine powder.Called freeze-fractur-ing, this method is espe-cially useful for tough,fibrous tissues such asskin, cartilage, cornea,etc.

The Bio-Pulverizer consists of a hole machined in a stainless steel base,into which fits a special piston. In a typical procedure, up to 10g ofanimal or plant tissue is hard-frozen in liquid nitrogen and placed in thepre-chilled Bio-Pulverizer. The piston is mounted on an easy to use trig-ger mechanism that is squeezed to deliver a blow to the brittle tissuereducing it to a fine powder. Freeze fracturing with the BioPulverizer isalso useful for extracting labile tissue metabolites and as a preliminarystep to other homogenization techniques.

☛ Bio-Pulverizer

Available in4” x 4”7” x 7”12” x 12”The solid ceramic topplate is easily cleaned &resistant to acid andalkali, while a reflectivewhite colour enhancessample visibility

☛☛☛☛☛ Cimarec Ceramic Top Stirrers

A strong magnetic coupling ensures stirring bar stays locked with drivemagnet, even in viscous solutions. The rugged cast aluminum body pro-vides stability and durability. A power light indicates when power issupplied to the speed control. Quiet stirring is achieved using a direct-drive motor/magnet system.

Stirrers come complete with one (2” x .38” diameter) Teflon-coatedstirring bar,

(Cimarec 1 supplied with one 1” x .31” diameter stirring bar), andgrounded three-wire cord and plug.

☛ Cimarec Ceramic Top Hotplates

Features:

• Solid ceramic top plate iseasily cleaned and resistantto acid and alkali.

• Cimarec’s reflective brightwhite color enhancessample visibility.

• Three sizes available: 4” x4”, 7” x 7”, and 12” x 12”.

• Integral ring-stand holderaccommodates 0.5” diam-eter support rod.

• Infinite temperature selection is provided by a percentage inputcontroller.

• Units reach maximum temperature of 538°C in eight minutes.• Accommodates sample weights up to 25lbs. on the 7” x 7” and 12” x

12” models and up to 10lbs. on 4” x 4” models.• Stirring models have a strong magnetic coupling to ensure that the

stir bar remains locked with drive magnet, even in viscous solutions.

Fits either a 5" x 9" table (for use with various tube sizes) or an 8" x 8"table (for microwell plates, blots, petri dishes, and cell flasks). Theunit comes standard with the 8" x 8" table.

This high quality NutatingMixer can be used for avariety of applicationsincluding mixing or re-suspension of specimens,tissue culture, immunoblots,etc. The mixer featuresvariable speed from 5 to40rpm and operates at a 20°tilt angle.

Our Vortex Mixer blends fluids quickly and thoroughly with a true vortexaction. Variable speed settings allow for precise mixing from a gentleshake to an aggressive vortexing action. The mixer has a durable alumi-num housing with non-slip rubber feet and can be set for continuousoperation or “touch” start and stop. Unit comes complete with cup-headfor single test tubes and a 3” platform head design for beakers, flasks,and multiple test tubes. A variety of adapters are also available to in-crease the vortexing capacity.

☛ Nutating Mixer

Specifications

Speed Range: Variable to 3,000rpmTemperature Range: 2°C to 40°CElectrical: 220V, 60HzDimensions: 5”H x 6”D x 5”W

☛ Vortex Mixer

The VX-100 vortex mixer is a valuable addition to any laboratory. Thecontinuously adjustable speed control meets a variety of applications.Lower speed settings allow for gentle mixing of samples while higherspeeds can be used for vortex mixing and resuspending of pellets. Thethree position control switch can be set for continuous operation ortouch control.

The general purpose cup attachment supplied with the VX-100 accepts avariety of different sized tubes. A variety of head attachments areavailable. The heavy duty construction of the unit ensures a long life andcombined with the rubber feet prevents “walking” across the benchduring operation.

Specifications

Speed Range: 0-3000rpmOperation Mode: Touch or ContinuousAmbient Operating Range: 4°C-65°CDimensions: 12cm x 15.5cm x 13cmWeight: 3.0kgElectrical: 220V, 50/60Hz

☛☛☛☛☛ VX-100 Vortex Mixer

The UNC-VM1000 Vortex Mixer is a heavy duty,variable speed mixer for a variety of differentlaboratory applications. It features a 2-positionswitch for continuous or “touch-on” modes and asoft rubber-mixing cup for single tube mixing.

Dimensions: 6.2" x 5.0" x 6.5"Weight: 9.4lbs

☛ Vortex Mixer


Orbital Shakers, Rockers, 3-D RockerINSTRUMENTS

Shakers

☛☛☛☛☛ BIOSHAKER PLUS

Speed Range: 25-250rpmPlatform Size: 11" x 11"Dimensions: 10.4" x 9.2" x6.0"Weight: 9.8lbsOrbit 1.9cm

☛☛☛☛☛ BIOSHAKER II

Heavy Duty

• 20" x 20" Platform• Skid Free Polyurethane Mat• Rugged construction• One year Warranty Parts

and Labor• Load capacity to 15 lbs• 40-400RPM• Suction cup feet (Can’t

Dance)

The BioShaker Plus is a compact, durable, variable speed orbital mixerthat will accommodate a wide variety of printed well slides, cultureplates, flasks and small test tube racks, as well as RPR and VDRL slides or96 well microtiter (immunoassay) plates. It features a 11"x 11" mixingplatform with a removable/replaceable rubber pad, variable speed of25-250rpm, a tachometer with an analog rpm indicator (adjustable), 3position switch for Constant On/Off/Timed Mixing, indicator alarm atend of timed mixing cycle, and hold down spring for small flasks.

The Orbit 300 accepts flasks, dishes or plates by means of interchange-able platforms and is ideal for most mixing, agitation, and vortexingapplications. The flask platform has spring-loaded retaining bars, mak-ing it flexible enough to accept a variety of different vessels.

Different platform configurations available: for microplates, with non-slip mats for holding dishes, for flasks, racks for holding tubes of variousdiameters.

Speed Range: 100-1,200rpmTimer: 99.5 minutes orcontinuousOrbit: 3mmMax. Load: 2kgDimensions (W x D x H): 26x 32 x 13cm

☛ Orbit Model 300 Platform Shaker

☛☛☛☛☛ Orbit™ Digital Orbital Shakers By Labnet

The Orbit™ line of digital orbital shakers is designed to satisfy the mostdemanding mixing and vortexing requirements across a broad range ofapplications. Heavy-duty construction combined with powerful motorsand advanced electronic controls enable the shakers to withstand thestresses of continuous operation, even in a cold room or incubator. Allmodels feature continuously variable speed controls, a wide assortmentof accessory attachments, and can be set for timed or continuous opera-tion.

☛ Orbit Models M60, P2 & P4 Microplate &Microtube Shakers

Speed Range:100-1,400rpmTimer: 99.5minutes orcontinuousOrbit: 3mmMax. Load:0.3kgDimensions:17 x 28 x 15cm

The Orbit P2 and Orbit P4 hold twoand four microplates respectively, bymeans of a contoured stainless steelplatform with retaining springs. TheOrbit M60 holds 60 x 1.5/2.0mlmicrocentrifuge tubes (or 0.2ml and0.5ml tubes with optional insertadapters).

☛☛☛☛☛ For Gels & Blots Single Or Double Rocker

• New Rockers are up to 10times quieter.

• Adjustable tilt from 0° to±40°

• Speed is adjustable from4 to 160 rocking motionsper minute.

• Solid-state speed control• Non-slip rubber on

platform decks.• Operates in temperatures

from 0°C to 65°C.

• Three dimensional motion• Variable Speed range (6-60

rpm)• Non-slip rubber mat• Single, double, large and

extra large platformsavailable (33 x 30 cm)

• Dimpled rubber mat(included) holds tubes inplace

• Elastic tie downs (included)to secure various contain-ers

• Safe for use in controlledenvironments(4 to 65ºC)

• Timer can be set for up to3 hrs or “continuous”

☛ Gyrotwister GX1000

Available in single & double platforms.


Water Baths - General, Shaking, ReciprocatingINSTRUMENTS

Water Baths

Standard Aquabaths arecontrolled by an adjustablehydraulic thermostat. Asecond independent hi-limit thermostat assumescontrol of bath tempera-tures should the controlthermostat fail. Tempera-ture is maintained even ifthe bath accidentally runsdry, thereby eliminatingheater burn out. Individualpilot lights indicate whichthermostat presentlycontrols temperature.Bath temperature is readfrom a glass thermometer(not supplied).

☛ Lab-Line Aquabath Waterbaths

Digital Aquabaths feature digital set temperature. Temperature is readfrom the LED display.

Each Aquabath can be used as a boiling bath with the addition of thePlexiglas gable cover (included).

Available in 2L, 3L, 6.7L, 14.6L, 22L, 33L, 44L capacities in boththe standard and digital formats.

Dual chamber baths are 14.6L (large chamber) and 6.7L (small cham-ber).

Gable covers available separately.

☛ ORS-200 Orbital & Reciprocating WaterBath Shaker

The ORS-200 water bath shakercombines both reciprocating (linear)and orbital shaking motions into oneunit. Temperature and shaking speedare digitally set and displayed, withthe control panel recessed to protectfrom spillage and accidentalalteration. Each bath is constructedof a stainless steel tank, with bottomdrain, in a durable outer case. Theheater and temperature sensors aremounted underneath the tank andthere is an adjustable safety over-temperature cutout.To change from orbital to recipro-cating motion, simply change theorientation of the tray. StainlessSteel lid available separately.

Specifications:

Tank Volume: 28.0LTemperature Range: 0 to 99°C in 0.1°C incrementsTemperature Uniformity: 0.1°CShaking Speed: 20 to 200rpm (Orbital)Orbital Radius: 9mmStrokes per Minute: 40 to 360 (Reciprocating)Linear Adjustable Stroke Length: 18mm, 28mm, 36mmShaking Tray Size: 14.75" x 9.25"Tank Dimensions: 19.75"L x 11.75"W x 7.75"H

Ideal for all general laboratoryapplications, the Premier WaterBaths utilize an integrated PIDmicroprocessor controller for easytemperature selection, rapid heat-up, and excellent stability. Theseamless, stainless steel tank hasrounded corners for easy cleaningand to minimize contamination. Allcontrols are recessed to protectfrom spills and there is anadjustable over-temperature safetycutout. Supplied with a stainlesssteel gabled lid, diffuser tray, andshelf.

☛ Premium Water Baths

Model: PB-600 PB-1400 PB-2800 PB-3600

Tank Volume: 6.0L 14.0L 28.0L 36.0L

Tank Dimensions 6" x 11.8" x 12.8" x 11.8" 20" x 11.8" x 25" x 11.8" x(LxWxH): 5.5" x 5.8" 7.5" 7.5"

Temperature Range: Ambient + 5°C to 100°C in 0.1°C incrementsTemperature Uniformity: 0.2°C

The SWB 5050 is an intelligent shakingwater bath designed to meet the needsof today’s biological laboratory. Itsbroad temperature range makes ituseful for a variety of applications,from growing bacterial cultures, tohybridization, to denaturation of DNAsamples.Specifications:Temp. Range: Ambient +5ºC to 85°C(to 105°C with stainless steel gablecover)Temp. Stability: 0.1°CTemp. Control Microprocessor, digitaldisplayOvertemp. Safety AdjustableBath Volume: 50L

☛ SWB 5050 Reciprocating Shaking Water Bath

Drain Tap Built in with front mount drain hoseShaking Speed Variable, 20 to 200 strokes/min.Chamber Construction Corrosion resistant stainless steelLid Construction Clear acrylic, hinged to housingTimed Operations Max. 100 hours or continuousHeating Capacity 2000WFlask Capacity 4 x 2L, 6 x 1L, 9 x 500 ml, 16 x 250 ml, 50 x 100 mlPlatform Height Adjustable, 10-90 mm

☛ Lab Companion Shaking Water Baths

Features:

• Digital PID Microprocessor Controller• Patented CLS (Custom Logical Safety) System• Volume Capacity 17L / 25L / 37L / 55L• Auto Tuning function for exact temp. control• 99h 59m Timer with Time Delay ON-OFF• Temperature Range Amb.+ 5.0 ~ 100.0 C• 20 to 180 rpm Shaking Speed• Reciprocating motion• Shaking amplitude 25, 30, 35mm (30mm Standard)• Standard offer : Complete Spring Wire Rack & Stainless

Steel Gable Cover• Optional : Test Tube Rack , Universal Platform for mounting

Flask Clamp, Spring Wire Rack, Handy Cooler


☛ Economy Series Refrigerated Heating Circulators

Model: F25-EC F34-EC

Bath Volume: 4.5L 20L

Bath Dimensions (W x L x D): 5" x 5.5" x 6" 9.5" x 12" x 6"

Operating Temperature Range: -25 to +100°C -20 to +95°C

Temperature Stability: 0.03°C 0.03°C

Heating Capacity: 1000 watts 1000 watts

Cooling Capacity @ 20°C: 350 watts 450 watts

Pump Pressure Capacity: 5 psi 5 psi

Pump Flow Capacity: 15 lpm 15 lpm

Overall Dimensions (W x L x H): 9.1" x 16.6" x 23.6" 15" x 23" x 25"

☛ TopTech Series Open Bath Heating Circulators -MP Series

The MP Series Open Bath Heating Circulators are a step up from the ECSeries. Digital temperature control via a splash-proof keypad, high andlow temperature warnings, bright LED temperature display, low liquidlevel protection, an RS232 interface, and high temperature safety cutoffare just a few of the standard features. Baths are constructed of stain-less steel and feature a working temperature range of 20°C to

100°C. Baths constructed of Plexiglas® or Makrolon® are also available.

Model: MP-5 MP-13 MP-19

Bath Volume: 5L 13L 19L

Bath Dimensions (W x L x D): 6" x 6" x 6" 7" x 12" x 6" 4" x 12" x 6"

Operating Temperature Range: 20-100°C 20-100°C 20-100°C

Temperature Stability: 0.02°C 0.02°C 0.02°C

Heating Capacity: 1000 watts 1000 watts 1000 watts

Pump Pressure Capacity: 1.75 psi 1.75 psi 1.75 psi

Pump Flow Capacity: 10 lpm 10 lpm 10 lpm

Overall Dimensions (W x L x H): 7"x13"x14" 15"x13"x14" 22"x13"x14"

The TopTech Series Refrigerated Heating Circulators feature PID tem-perature control. High and low temperature warnings are standard, aswell as a high temperature safety cut-off and low liquid level protection.For programmability, units can be connected via the RS232 port to acomputer. Baths are constructed of stainless steel.

☛☛☛☛☛ TopTech Series Refrigerated Heating Circulators

Model: F25-EC F34-EC

Model: F25-MD F34-MD

Bath Volume: 4.5L 20L

Operating Temp -25 /+200°C -30/150°C

Temp Stability: 0.01°C 0.01°C

Heating Capacity: 1000 watts 1000 watts

Cooling Cap: 350 watts 450 watts

Pump Pressure Cap: 5 psi 5 psi

Pump Flow Cap: 5 lpm 15 lpm

Refrigerated & Heating CirculatorsINSTRUMENTS

Water Baths

☛ 6 Liter Mini Water Bath

Labnet’s 6 Liter Water Bath is solidly constructed with a stainless steel,seamless chamber that resists corrosion. A stainless gable cover is includedto minimize evaporation. The optional non-toxic (non-mercury)thermometer clips onto the gable cover for accurate temperaturemonitoring. The chamber accommodates plastic tube racks.

Specifications:

Exterior dimensions (W x D x H) 14x11x13 in

Interior dimensions 12.6x6.7x6.9 in

Weight 17 lbs / 7.7 kg

Chamber capacity 6 liter

Temperature range +5 °C to 100 °C


These standard ovens meet thecapacity demands of medium to highthroughput laboratories. Thestandard rotisseries in these ovensare unique in that they will hold 50mltubes, eliminating the need topurchase a separate rotisserie forprocessing strips and small blots.The ProBlot 24 features twoindependently controlled chambers,allowing for separate applications tobe carried out simultaneously withinthe same unit. All three of thestandard ovens feature a corrosionresistant stainless steel interior.Optional accessories include arocking platform, rotisserie for70mm bottles, and a rotisserie forvertically positioned 50ml tubes.

☛ ProBlot™ 6, 12 and 24

The availability of the accessories combined with the broad tempera-ture range of these ovens, makes them useful for hybridization andwashing of blots as well as other applications requiring strict tempera-ture control

All ProBlot Hybridization Systems are supplied with a starter kit con-

taining 2 of Labnet’s

ProBlot bottles and a pack of hybridization mesh. The bottles are

constructed of heavy walled borosilicate glass with a flat bottom. The

35mm diameter bottles are available in a variety of lengths.

Specifications:

ProBlot 6 ProBlot 12 ProBlot 24

Catalog number H-0600 H-1200 H-2400

Bottle capacity 6 large, 12 large, 24 large,

12 small 24 small 48 small

Rotisserie/rocker 4 to 20rpm 4 to 20rpm 4 to 20rpm

speed

Temp. range Ambient + Ambient + Ambient +

5º to 80ºC 5º to 80ºC 5º to 80ºC

Temp. Microprocessor/

control/display digital do do

Temperature

resolution/ 0.1º/±0.5ºC 0.1º/±0.5ºC 0.1º/±0.5ºC

Uniformity

Exterior dim. 18.4x17x17.4 in. 18.4x17x17.4 in. 20x16.5x27.7 in.

(WxDxH)

Chamber dim. 14.4x11x11.3 in. 14.4x11x11.3 in. 14.4x11x11.3 in.

(WxDxH)

The HB-500 Minidizer is a personal,desktop hybridization oven that is idealfor use in laboratories with low use andlimited space requirements. Micropro-cessor controls allow for consistent,repeatable results and the chamberinterior is stainless steel with a 3/8"beta-blocking acrylic cover. The coverswings down for direct access to thebottle carousel, which can be removedfrom the chamber for the easyinsertion or removal of bottles.

☛☛☛☛☛ HB-500 Minidizer

Minidizer Specifications:

Temperature Range: Ambient to 80°CRotation Speed (Fixed): 12rpmBottle Sizes Accepted: Four 35 x 150mm

Eight 50ml or 15ml Conical TubesDimensions: 9"H x 13"W x 8"D

Mesh sheets may be used to facilitate membrane handling and improveoverall results. They keep membranes from overlapping, support themduring handling, and allow larger numbers of membranes to be hybrid-ized effectively in a single bottle. Available in Rolls and Sheets.

Our high strength glass bottles are available in many size configurationsto suit various hybridization applications efficiently. Manufactured oflow beta emitting borosilicate, they reduce exposure to radioactivity.Exposure is further limited by the leak-proof closure system.

Red Cap - Temperature Resistance: -45°C to +200°C (Teflon Seal)

Blue Cap - Temperature Resistance: -40°C to +140°C

(O-Ring Seal)

Available in different sizes.

☛ Hybridization Mesh and Bottles

☛ HL-2000 HybriLinker

The innovative HL-2000 HybriLinker System combines the benefits of ahybridization oven and a UV crosslinker (254nm) in one self-containedunit.

Hybridizer Features: Temperature control (ambient to 100°C), variablespeed control (10 to 18rpm), offset bottle-positioning, multiple bottlesizes (twenty 35 x 150mm, ten 35 x 300mm, or six 75 x 300mm tubes, ordifferent arrangements of each), stainless steel internal construction,and optional stainless steel rocker plate. Also available is an optionalover-sized bottle rotisserie, which accommodates six 70 x 300mm bottles.

Crosslinker Features: Microprocessor controlled UV feedback system,preset or user-selected UV energy exposure or UV time exposure, short-wave 254nm UV, maximum UV energy setting of 999.900 microjou

INSTRUMENTSHybridization OvensHybridization Ovens & Accessories


More than just a hybridizationoven, the ProBlot 12S has an or-bital shaking mechanism built-into the base, expanding its use toa variety of other applications.The rotisserie in the ProBlot 12Swill hold 12 large or 24 smallbottles as well as 50ml tubes. Thebase of the shaker accommodatesplates and dishes. The flask plat-form, available separately, issupplied complete with 6 x 250mlflask clamps.

An optional rocking platform isalso available for use in the cham-ber when the rotisserie is re-moved.

☛ ProBlot™ 12S

Optional accessories include the flask platform with clamps, rockingplatform and rotisseries for other sizes of bottles

SpecificationsTemperature range Ambient +5º to 80ºCBottle capacity 12 large, 24 smallTemperature control/display Microprocessor/digitalRotisserie/rocker speed 4 to 20rpmTemperature resolution/uniformity 0.1º/±0.5ºCShaker speed 0 to 300rpmExterior dimensions (WxDxH) 18.4x17x17.4 in.Timer 3 hours max. with holdChamber dimensions (WxDxH) 14.4x11x11.3 in.

All ProBlot Hybridization Systems are supplied with a starter kit

containing 2 of Labnet’s ProBlot bottles and a pack of hybridization

mesh.

☛☛☛☛☛ ProBlot™ Jr.

The ProBlot Jr. is a unique,personal sized oven.Requiring less than onesquare foot of benchspace,it has a capacity for foursmall bottles. The oven isportable and easily moved asneeded. The acrylic dooropens upward for easysample access. Optionalrotisseries hold 15 and 50mltubes. Standard accessoriesinclude two small bottles, onepack of mesh and a 35mmrotisserie.

Specifications ProBlot Jr.Bottle capacity 4 smallRotisserie/rocker speed 4 to 20rpmTemperature range Ambient +5º to 80ºCTemperature control/display Microprocessor/digitalTemperature resolution/uniformity 0.1º/±0.5ºCExterior dimensions (WxDxH) 10.75x11.25x7.25 in.Chamber dimensions (WxDxH) 6.5x6.x6.5 in.

☛ Electroporation Cuvettes

Our range of HiMaX Electroporation cuvettes are designed to maximisemolecular electroporation and electrofusion efficiencies for Bacteria,Yeast, Insect, Plant and Mammalian cells. Each batch of cuvettes has toundergo rigorous testing at several stages during the manufacturingprocess for engineering tolerances, biocompatibility and sterility, priortheir being Quality tested for optimal and reproducible impedance mea-surements.

COMPATIBILITY

The cuvettes are compatible with most electroporation systems

BIO-CONTROLLED

All batches are checked to optimize the Bio and Transfection compatibil-ity, with stringent use of high quality grade polycarbonate and Highgrade chemicals to ensure consistent uniform pulse generation and im-proved gene transfer.

HIGH TOLERANCE MOULDING

The moulding process ensures extremely high tolerances so that the elec-trodes have a consistent gap and parallel configuration. The electrodesare also cleaned chemically and physically to fully optimize the cuvettefor high transformation efficiencies.

LOW DEAD VOLUMES

All 1mm and 2mm cuvettes have a tapered V bottom so that reducedsample volumes can be used while aiding sample pick up and minimizingdead volumes.


EP-101 1mm cuvette individually wrappedand sterile 50 Nos.

EP-201 1mm cuvette with long electrodeindividually wrapped and sterile 50 Nos.


EP-202 2mm cuvette with long electrodeindividually wrapped and sterile 50 Nos.


EP-101 Disposable sterile individuallywrapped plastic pipettes 50 Nos.

Hybridization Ovens/ Electroporation CuvettesINSTRUMENTS

Hybridization Ovens/ Electroporation Cuvettes


Economical benchtopmodels providemidrange (302nm) UV ineither single intensityor High/Low Intensitysettings. Select the Highsetting for analyticaldocumentation work orthe Low setting forreducing photonickingor photobleaching of gelsamples while doingpreparative work.

☛ Benchtop UV Transilluminators

The 2UV and 3UV Benchtop Transilluminators deliver multiple UVwavelengths in one compact unit. The 3UV’s patented design provides302nm, 365nm and 254nm in one compact model - excellent forpremium lab space. Work surface area: 20x20cm.

• Longwave UV Ideal For Viewing Stained Gels• Midrange UV Provides A Wavelength Sensitive For Nucleic Acid Visual-

ization; Increased Fluorescence For Photodocumentation Work• Shortwave UV Can Be Used To Irradiate Samples• Compact Version Provides Three Ultraviolet Wavelengths In One Unit

Dimensions for all Benchtops (Except 3UV): 13.25”W x 9.5”D x 4.75” H

3UV Dimensions: 14”W x 11”D x 6”H

Model Description Filter Size Wavelength

M-15 Hi/Lo Intensity, 220V 15 x 15cm 302nmM-15E Single Intensity, 220V 15 x 15cm 302nmM-20 Hi/Lo Intensity, 220V 20 x 20cm 302nmM-20E Single Intensity, 220V 20 x 20cm 302nmM-26 Hi/Lo Intensity, 220V 21 x 26cm 302nmM-26E Single Intensity, 220V 21 x 26cm 302nmLM-20E 2UV, 220V 20 x 20cm 302nm/365nmLM-26E 2UV, 220V 21 x 26cm 302nm/365nmLMS-20E 3UV, 220V 20 x 20cm 254nm/302nm/365nmLMS-26E 3UV, 220V 21 x 26cm 254nm/302nm/365nm

☛ UV/White Light Converter Plates

A specially designed plate converts a transilluminator’s ultraviolet radia-tion to white light for viewing protein gels; Coomassie blue stained orsilver stained media. The Converter Plate’s uniquely phosphored Plexiglasassembly (patent pending) converts the UV radiation via a white dif-fuser. The Plexiglas is mounted in a durable scratch-resistant metalhousing for durability. Handles are situated on two sides of the plate foreasy handling. Sizes available are 21x26 cm and 20x40 cm

☛ Visi-Blue Converter Plates

A specially designed plate converts 302nm to 480nm blue light for usewith SYBR Green, SYPRO Orange, and GFP stains. Sizes available are21x26 cm and 20x40 cm.

☛ Handheld UV LampsThese Handheld UV Lamps featurean ergonomically designed handlefor easier handling and comfort.They are available in longwave,shortwave, midrange, or multibandlongwave/shortwave UV models. Anoptional stand is available forbenchtop use and the lamps can alsobe used with the C-10 ChromatoVueViewing Cabinet for viewingmaterials in a darkened environ-ment.

• Preset And Manual Controls ForUV Or Time Exposure Settings• Internal MicroprocessorMeasures And Controls UVOutput, Insuring MaximumEnergy Efficiency• Internal UV Sensor Is Cali-brated With Traceability To NISTStandardsUse shortwave for sterilization,bonding DNA; use midrange forgel electrophoresis; use longwavefor UV curing.

☛ UV Crosslinkers

LED display above the touch panel continuously displays remaining timeor energy settings. Clear door window allows viewing of the process whileblocking the UV radiation. A safety shut off system further protects theuser from exposure to UV. Available in 254 nm, 302 nm and 365 nm. UVwavelength is interchangeable in any model by changing the UV tubes andcalibrating the unit to the new wavelength with the UV sensor (orderedseparately).

☛ UV/White Light Transilluminators

UV/White Light Transilluminatorsgive white light and 302nm midrangeUV side by side for viewingCoomassie Blue stained gels, silverstained protein gels, autoradio-graphs and microtiter plates onwhite light side. UV side can be usedfor viewing gels such as ethidiumbromide stained gels. UV BlockingCover included.

Transilluminators Can Be Used With GDS & Doc-It Systems. The filtersize is 20x20 cm and avaioable in 302nm/white, 365 nm/white and 302/365nm/White.Model TLW-20 utilizes longwave and white light side by side.Model LMW-20 houses both longwave and midrange on one side andwhite light on the other, providing varied wavelengths for differentapplications. Easily switch between light sources with the rockerswitch.Dimensions: 19”W x 13.25”D x 5” H.Replacement tubes available.

☛☛☛☛☛ High Performance UV Transilluminators

The higher watt tubes allow thetubes to be moved further fromthe filter surface resulting inincreased light diffusion while atthe same time maintaining thehigh UV intensity.Models are available withmidrange or longwave ultraviolet.Dimensions: 19.13”W x 13.25”D x5.63”H (Height includes cover)Replacement tubes available.

Model Cat. No. Volts Intensity Wavelength Filter Size

TFM-20 95-0286-01 220 Hi/Lo 302nm 20x20cm

TFM-26 95-0285-01 220 Hi/Lo 302nm 21x26cm

TFM-30 95-0302-01 220 Hi/Lo 302nm 25x30cm

TFM-40 95-0283-04 220 Hi/Lo 302nm 20x40cm

TFL-40 95-0283-01 220 Hi/Lo 365nm 20x40cm

INSTRUMENTSTransilluminators/CrosslinkersUV Transilluminators and Crosslinkers


Cuvettes, Gloves, LabelsINSTRUMENTS

General Lab Equipments

☛☛☛☛☛ Cuvettes, Semi-Micro (1.5 ml)Polystyrene (VIS)

These exceptionally fine quality polystyrene cuvettes are deisgned forassays throughout the 340nm to 750nm visible spectral range. A standard1cm light path and recessed windows provide maximum light transmis-sion.1 pack contains 50 nos.

Dimensions: 12.5mm x 12.5mm x 45mmWindows: 5mm x 23mmLight Path: 10mm

☛ Cuvettes, Macro & Semi-MicroPolystyrene (VIS)

Premium grade polystyrene cuvettes fit most spectrophotometers, in-cluding Beckman, Perkin-Elmer, Gilford, Hitachi, Varian, and Shimadzu.Both the 4ml (macro) and the 1.5ml (semi-micro) have two clear sides.Applications include general spectroscopy in the visible region, physi-cian office analyzers, and enzyme reaction rate studies. 1 pack contains1000 nos.

☛☛☛☛☛ Cuvettes, Semi-Micro (1.5ml)Methacrylate (UV-VIS)

Injection molded from a superior grade of methacrylate, these cuvettesdesigned for accuracy throughout the UV-VIS spectral range from 285nmto 750nm. Excellent optical qualities throughout the spectral range makethem perfect for scanning small volumes, enzyme rate reactions, andstandard curve determinations. 1 pack contains 50 nos.

☛ Cloth Glove Liners

These 100% cotton glove liners fitcomfortably under plastic gloves toensure maximum comfort whileprotecting sensitive skin from theirritating effects of latex or donningpowders. Available in packs of 1 Gross,in small, medium and large sizes.

☛ GlovesShark SkinThe Powder Free Latex Glove

• Non-Sterile• Superior Texture• Puncture Resistant• Sleek Fit• Jaw-Like Grip — EvenWet• Durable and Comfortable

Quantites: 100 Gloves PerDispenser Box; 10 BoxesPer CaseSmall, Medium, Large

Diamond Grip is our most popularpowder-free, textured exam glove.It stands alone as a perfect glovefor any application. It’s built toughfor greater reliability and comfort.Plus, it’s powder free to helpreduce the risk of skin irritation.

Quantities: 100 Gloves PerDispenser Box; 10 Boxes Per CaseSmall, Medium, Large

☛ Diamond Grip

☛☛☛☛☛ Diamond Grip PlusFor those who prefer the grip of a fullytextured glove, Diamond Grip Plus isthe glove of choice.And for those healthcare professionalswho require greater tactile sensitivityfor instrument manipulation oradministering medical care, DiamondGrip Plus is a perfect fit. DiamondGrip Plus is powder-free to help reducethe risk of skin irritation.Quantites: 100 Gloves Per DispenserBox; 10 Boxes Per Case

Small, Medium, Large

☛☛☛☛☛ Cryo-Babies® & Cryo-Tags®☛ Labels For Cryogenic Storage

Cryo-Babies® and Cryo-Tags® willwithstand conventional freezing andcryogenic storage in vapor-phase andliquid nitrogen. These heat resistant,ultra-flexible polyolefin labels withacrylic adhesive are chemically inert andadhere to all plastics, glass, and metal.The labels will withstand boiling waterbaths at 100°C and dry heat up to 150°Cwithout cracking, peeling, or degrading.They are water and moisture resistant,and will resist water soaking for at least24 hours at room temperature.

Tough-Spots® are chemicallyinert white polyvinyl labels thatadhere to all plastics. Theselabels withstand boiling waterbaths, conventional andcryogenic freezer tempera-tures, most organic solvents,caustic agents, and otherchallenges without peeling,becoming brittle, or degrad-ing. The 3/8” Spots will fit 0.5-2.0ml microtubes and the 1/2”Spots are ideal for 1.5-2.0mlcontainers.

Available in different colours.

☛☛☛☛☛ Tough-Spots®☛ Pre-Cut Peel-Off Round Labels for

Microcentrifuge Tube Tops

Different varieties of Tough-Tags are available for different applica-tions. Laser Tough Tags used for printing directly from Laser printer.Teeny tough tags used for 0.2 ml PCR tubes, Sidewall tags used for wallsof microplates, petri dishes and their lids. General-purpose tags arelarger and used for a wide variety of laboratory applications.

• Temperature Resistant (canbe used in autoclaves, boilingwater baths and freezers• Resists Solvents & CausticReagents• Ideally Sized• Centrifuge Compatible• Scuff & Smear Resistant• Multiple UsesThe 1.28" x 0.50" size is idealfor 1.5-2.0ml microtubeswhile the 0.94" x 0.50" size fits0.5-2.0ml microtubes.

☛☛☛☛☛ Tough-Tags

☛☛☛☛☛ The Pre-Cut, Peel-Off Labels Perfectly Sized toFit Microcentrifuge Tubes and Other LaboratoryContainers


INSTRUMENTSGeneral Lab EquipmentsSmall and Important tools that you need in the lab.

☛ The Bioslide Advantage

Precision CT - Clean• Ready for surface chemical

modification and coatingprocess

Precision CT - Amine• Stable, covalent coupling of

primary amine on slide surface• Ready for DNA/RNA printing• Quality tested for oligonucle-

otide immobilization• Ideal for custom in-house

printing

Precision A - Aldehyde• Stable, covalent coupling aldehyde on slide surface• Modified with primary amines form Schiff-based covalent bonds• Ideal for protein immobilization

Precision E - Epoxy• Stable, covalent coupling epoxy on slide surface• Superior attachment of amino-modified oligonucleotides• Ideal for synthetically fabricated oligonucleotides and PCR products

Precision L - Lysine• Ready for DNA/RNA printing• Hand screened and tested• Ideal for custom in-house printing

Starting with high quality low fluorescent glass, the Bioslide UltraClean,Amine, Aldehyde, Epoxy, and Poly Lysine-coated slides are manufac-tured in a Class 100 Cleanroom and have been tested to ensure surfaceuniformity, consistent contact angle and slide-to-slide reproducibilityduring spotting and hybridization processes. Moreover, the nearly par-ticle-free environment ensures high-quality surface treatments whichbind an optimal amount of probe, optimize volume transfer, minimizenonspecific binding of labeled targets, promotes uniform spot morphol-ogy, and assures the consistent performance of every pre-coated slide.

UVP’s Gel Tools are useful forresearchers working with gels ontransilluminators. The Gel-Cutter’sedge allows for cutting and removalof gel material. The Gel-Scooper,made of strong acrylic with abeveled edge, is designed for easytransfer of gels from electrophoresisequipment to viewing equipment.Gel-Trays, made of UV transmittingPlexiglas, can be used for movinggels to the transilluminator.

The tray protects the transilluminator’s filter surface from scratches.

Side panels on the Gel-Tray, angled at a 45 bend, extend upward fromthe tray surface for easy handling. The Gel-Ruler has centimeter mark-ings that fluoresce under 365nm and 302nm UV wavelengths providingreference marks for DNA analysis.

☛ Gel Tools

Air vents are located on each side of the goggles to allow air flow whileblocking UV. The faceshield covers the face and neck area.

Eye and face protection areessential for anyone working withultraviolet light sources. Choosefrom three styles of comfortableeye/face protection: spectacles,goggles, or faceshield. Spectaclesare made of impact resistantpolycarbonate. Goggles areconstructed of specially-formulated plastic givingoptimum viewing contrast andlessened eye fatigue.

☛ UV Protective Eyewear

• Made of durable polysty-rene. Unlike glass alternatives,disposable Lazy L Spreadersare non-breakable and do notpose a safety hazard.• L-shaped design forconvenient and lazy spreading;just turn the petri dish 360degrees to provide smooth andeven sample distribution.• Pre-sterilization eliminatesflaming or autoclaving andreduces the risk of contamina-tions. No more wasted timewaiting for glass or metalspreaders to cool before use.

☛☛☛☛☛ Lazy L Spreaders

They can be used under hoods without danger, are color coded for easeof size identification, and are extra long to reduce the risk of contami-nation.

Total Length: 22.7cm (9”) Loops available in 1µl and 10µl.

Our Innoculating Loops and Needles are smoothand flexible to facilitate uniform, smoothstreaking without damage to media/gelsurfaces. The needles are straight and suitablefor removal of single colony specimens. Theloops are extra-smooth and constructed ofpolypropylene and rubber.Both the loops and needles are packed sterile insafe, tamper-proof, zip-seal resealable bags.Cross contamination due to improper steriliza-tion is eliminated.

The Replica Plating Device utilizes alocking ring that secures sterilevelveteen squares onto a PVC cylinderfor easy control of the plating device.It may be disinfected between useswith a brief rinse in ethanol orchlorine bleach. The aluminum ring is102mm in diameter and the PVCcylinder is 55mm high. For use with90-100mm petri dishes.

Includes one bag of 12 Velveteen Squares (6" x 6"). These squares arealso available separately.

☛☛☛☛☛ Replica Plating Device

☛ Innoculating Loops and Needles


Crystal Screening KitsX-RAY CRYSTALLOGRAPHY

Crystal Screens

☛ JBScreen Crystal Screening Kits

The JBScreen Crystal Screening Kits 1-10 cover 240 of the most promi-nent buffers for protein crystallization. Their compositions result fromdata mining of several thousands of crystallized proteins. JBScreen rep-resents the statistically most successful buffers that yielded proteincrystals suitable for X-ray diffraction. Just check out the formulationsof JBScreen, you will probably recognize your conditions or very similarones.

The JBScreen buffers are principally ordered by type and concentra-tion of the precipitant. This allows easy extraction of all relevant infor-mation and is already a first step to a refinement: Once you get a hit,you immediately see the effects of the neighboring conditions. Subse-quent fine-tuning of preliminary hits will be much more efficient.

Each kit contains 24 buffers, delivered in 0.7 ml sterile aliquots, with 10units per kit.

Alternatively, the JBScreen Mixed kit contains one single shot of all the240 buffers. The 6x4 format of the units allows a convenient 1:1 setup for24 (or multiples thereof) well plates. It’s easy to use – every ampoule andthe unit’s package has a “coordinate system” (A1–A6 ... D1–D6), thusalmost certainly eliminating errors.

• No pipetting, just break off the ampoule’s top and use thebuffer aliquot –

• JBScreen is always fresh and sterile unlike common bulksolutions

• Reproducible- no evaporation of solvent


CS-101 JBScreen Kit 1 1 Kit










CS-111 JBScreen Kit Mixed 1 Kit

☛ JBScreen Refill Kits

The JBScreen Refill Kits 1 – 10 contain 24 x 10 sterile aliquots of theJBScreen buffers, supplied as 10-ampoule blocks, with 0.7 ml per am-poule. The JBScreen Refill Kits are ideal if you are already using JBScreenand would like to re-use its packages and 24-ampoule units. Simply breakoff the ampoules from the blocks and re-fill your JBScreen kits!


CS-101S JBScreen 1 Refill Kit 1 Kit










☛ JBScreen bulk kits

The JBScreen bulk kits contain all 240 solution of the JBScreen system insterile 10 ml quantities. These kits are especially designed for users whoprefer reservoir solutions that are different from the standard 0.7 mlformat, such as labs that use crystallization robots.


CS-101L JBScreen 1 bulk 1 Kit










☛ JBScreen HTS

JBScreen HTS contains the formulations of the JBScreen system, adoptedto fit the 96-well format. JBScreen HTS is designed for high throughputcrystallization applications using multi-channel pipettes or crystalliza-tion robots. Each JBScreen HTS 96-well master block is pre-filled with 96sterile crystallization buffers, with either 1.0 ml (S size) or 1.7 ml (Lsize) per well and is sealed with indexed cap mats.

JBScreen HTS will enable you to combine high-throughput crystalliza-tion techniques with the most efficient and logical screen available!


CS-201S JBScreen HTS I S (1.0 ml per well) 1 Kit

CS-201L JBScreen HTS I L (1.7 ml per well) 1 Kit

CS-202S JBScreen HTS II S (1.0 ml per well) 1 Kit

CS-202L JBScreen HTS II L (1.7 ml per well) 1 Kit

☛ JBS Membranes

The JBScreen Membrane Screens 1 - 3 cover 72 of the most promisingreagents for crystallization of membrane proteins. Their compositionwas devised by analyzing crystallization conditions of successfully deter-mined membrane protein structures. The JBScreen Membrane crys-tallization buffers are principally ordered by type and concentration ofthe precipitant. Like in the case of JBScreen Classic, this allows easyextraction of all relevant information and is already a fi rst step to a refinement of the crystallization process: Once you get a hit, you immedi-ately see the effects caused by the composition of the neighbouringconditions. Subsequent fi ne tuning of preliminary hits will be much moreeffi cient.


CS-301L JBScreen Membrane 1 1 Kit




X-RAY CRYSTALLOGRAPHYCrystal OptimizationWizard Screens, PEG Screens, Buffers

☛ Emerald BioStructures Screens

deCODE’s Emerald BioStructures growth matrices cover a range of crys-tallization conditions of varying buffers, salts, precipitants, and pH.Sixteen different precipitants are used, including volatile reagents suchas ethanol, non-volatile reagents such as PEG 8000, and salts such asammonium sulfate.

Each crystallization Matrix Contains:

• 48 unique formulations, 10 ml each, prepared with ultrapure chemicals and water (18.3 MOhm), followed by sterile0.22-micron filtration into sterile polypropylene tubes.

• Two oils, 5 ml each of miscible light and heavy oils for use inmodulating vapor diffusion rates.

☛☛☛☛☛ Wizard I + II

Emerald BioStructures Wizard Screens are highly effective random sparsematrices for the crystallization of biological macromolecules (proteins,nucleic acids, peptides and combinations thereof). Sixteen differentcrystallants and eleven different buffers, ranging from pH 4.5 to pH10.5, ensure a broad sampling of crystallization space.


EBS-WIZ-I Wizard I 1 Kit

EBS-WIZ-II Wizard II 1 Kit

EBS-WIZ-F Wizard I + II 1 + 1 Kit

EBS-BWZ Wizard I + II (in 96 well format) 1 kit

☛ Ozma™ PEG-Series 48-Salt: 1K, 4K, 8K and 10 K

The Ozma™ PEG-Series 48-Salt Screens are formulated using one offour different molecular weight PEG’s; 30% (w/v) PEG 1000, 20% (w/v)PEG 4000, 20% (w/v) PEG 8000, or 10% (w/v) PEG 10,000 plus 48 differ-ent salts. These protein crystal growth matrices provide excellent cov-erage of a broad range of PEG molecular weights (1K to 10K) at knownoptimal concentrations for protein crystal growth, in combination with48 different salts. The cationic components of the salts cover Ammo-nium, Calcium, Lithium, Potassium, Sodium, Magnesium, and Zinc. Theanionic components the salts cover for Acetate, Chloride, Fluoride, For-mate, Iodide, Nitrate, Phosphate (mono- and di-basic), Sulfate, Tar-trate, Thiocyanate, Citrate, and Isothiocyanate.

Full Ozma™ PEG-Series 48-Salt in 2 x 96 Well Matrix Block Plates:

Contains 192 unique formulations comprising all 4 sets of our Ozma PEG1K, 4K, 8K, and 10K screens, each with 48 different salts. Used for thecrystallization of biological macromolecules in PEG-1000, PEG-4000, PEG-8000 and PEG-10000 by varying the salt type.

Cat. No Product Qty

EBS-PEG-1 Ozma™ PEG 1K 48-Salt 1 Kit




EBS-PEG-48F Full Ozma™ PEG-Series 48-Salt 4 Kits

EBS-PEG-48BLK Full Ozma™ PEG-Series 48-Salt 2 Blocksin two 96 Well Matrix Block Plates

☛ JBScreen Basic

One approach to find suitable crystallization conditions is the Sparse-Matrix method. This method involves screening with an intentional biastowards conditions, which have been proven successful in the crystalliza-tion of biological macromolecules. In 1991, Jancarik and Kim published50 conditions, which were derived from previously crystallized pro-teins. They are designed to fit the 24-well plate format and like in allother JBScreen crystallization kits, cacodylate buffers were replacedby MES. JBScreen Basic contains 96 unique reagent mixtures for screen-ing a wide range of pH and various salts and precipitants. Each conditionof the four kits is supplied in 10 ml quantities. Furthermore, JBScreenBasic HTS contains all 96 conditions in a pre-filled deep well block.


CS-121 JBScreen Basic 1 (Diol and PEG based) 1 kit

CS-122 JBScreen Basic 2 (PEG based) 1 kit

Cs-123 JBScreen Basic 3(Polymer, Alcohol, salt based) 1 kit

CS-124 JBScreen Basic 1 (salt based) 1 kit

CS-203S JBScreen Basic HTS (1.0 ml per well) 1 kit

CS-203L JBScreen Basic HTS (1.7 ml per well) 1 kit

☛ JBScreen Buffer Kits

JBScreen Buffer Kits are designed for convenient reproduction andoptimization of crystallization conditions. The solutions can be used toreformulate conditions of the JBScreen family, e.g. JBScreen Classic,JBScreen Basic, JBScreen Cryo, and other commercially available crys-tallization screens. Furthermore, JBScreen Buffer Kits can be employedfor the straightforward preparation of custom screen solutions for therefinement and optimization of initial crystallization conditions. TheJBScreen Buffer Kit formulations will help to save time preparing accu-rate and high-quality reagents for the reproducible production of singleprotein crystals.

The JBScreen Buffer Kits contain ready-made buffer solutions withpreset pH values.

• JBScreen Buffer Kit Sodium Acetate, pH 3.5 – 5.6

• JBScreen Buffer Kit Sodium Citrate, pH 3.7 – 6.0

• JBScreen Buffer Kit MES, pH 5.6 – 6.7

• JBScreen Buffer Kit HEPES, pH 6.8 – 8.2

• JBScreen Buffer Kit Tris-HCl, pH 7.1 – 9.0

Each buffer is provided as a 1.0 M stock solution and supplied in10 ml volumes.

☛ JBS Single Stocks

Single stock solutions of the JBScreen components, i.e. buffers, saltsand precipitants, are ideal for the optimization of your crystallizationconditions. JBScreen Single Stocks are ready for use: the concentra-tion is adjusted and they are sterile fi ltered.Buffers, Salts, Precipitants available.


CO-101 JBScreen Buffer Kit Sodium Acetate 1 Kit

CO-102 JBScreen Buffer Kit Sodium Citrate 1 Kit

CO-103 JBScreen Buffer Kit MES 1 Kit

CO-104 JBScreen Buffer Kit HEPES 1 Kit

CO-105 JBScreen Buffer Kit Tris-HCl 1 Kit


Solubility, Pre-crystallization Screen , Detergents, AdditivesX-RAY CRYSTALLOGRAPHY

Crystal Optimization

☛ JBS Solubility Kit

The JBS Solubility Kit is a pre-crystallization screen to improve thecomposition of the initial protein buffer solution prior to performingcrystallization set-ups. Studies have shown that aggregation of the pro-tein may inhibit nucleation and crystal growth. Therefore, the JBS Solu-bility Kit has been developed to investigate protein samples towardstheir homogeneity and monodispersity prior to crystallization trials,employing hanging drop vapour diffusion experiments combined withdynamic light scattering.

The JBS Solubility Kit contains a set of 24 buffer solutions at differentpH-values for setting up hanging drop vapour diffusion experiments inorder to monitor the aggregation and precipitation of the protein sample,and a set of 14 additives used for further optimization employing dy-namic light scattering.

☛ ADDit™ Additive Screen in 96 Well Block Plate

The ADDit™ Additive Screen is a set of 96 different small molecule and saltsolutions that have been found to be effective in aiding the crystalliza-tion of biological macromolecules. 1.0 ml each prepared with ultra-purechemicals and water (18.2 Megohm-cm) followed by sterile 0.22 micronfiltration into a sterile 96 deep well matrix block plate that is sealed witha sterile reusable matte cover.

☛ pHat™ Buffer Screen

The pHat™ Buffer Screen is a set of 96 different buffer solutions encom-passing 12 different buffer systems at 8 different pHs each ranging insteps of 0.4 pH units from ±1.4 pH units about the buffer pKa. Thebuffers are organized according to their final pH so as to facilitate thesetup of subsequent refinement screens. The pHat™ buffers can alsosimply be added to any other 96 formulation screen to add anotherchemical dimension to the search for optimized crystal growth condi-tions.

pHat™ Buffers in 96 Well Block Plate: The buffers are delivered in a 96deep well block plate filled with 1.7 ml of each buffer solution at 10x therecommended final concentration.

☛ JBS Methylation Kit

Surface engineering of biological macromolecules provides a powerfultechnique to deal with proteins which are reluctant to crystallize orwhich yield poorly diffracting crystals only. The JBS Methylation Kitoffers a straightforward tool for the selective methylation of lysineresidues. The method does not require laborious cloning/expression/purifi cation but chemically substitutes the protons of the amino groupof lysine residues with methyl groups.


CO-310 JBS Solubility Kit 1 Kit


ADDIT-1 ADDit™ Additive Screen 1 Block


PHAT-T1 pHat™ Buffer Screen 1 Kit

PHAT-B1 pHat™ Buffers 96 well Block 1 Block


CS-510 JBS Methylation Kit 1 Kit

☛ JBScreen Plus

To select the formulations of the JBScreen Plus solutions, numerousreports on the use of additives to improve the quality and size of mac-romolecular crystals have been evaluated.

JBScreen Plus comprises 5 kits, including kosmotropic

(structurestabilizing) and chaotropic (structure-disturbing) additives,salts, volatile and non-volatile organics and other compounds. Each kitcontains 24 different additives, supplied in ready-made aliquots (100 µleach, except the JBScreen Plus Volatiles kit, containing 1 ml each), withadjusted concentration and sterile fi ltered. All solutions come in tightlyclosing glass vials that will keep them fresh and sterile.

☛ JBScreen Detergents

The JBScreen Detergents Kits 1 and 2 cover a great variety of deter-gents that have been successfully used for the crystallization of mem-brane proteins. Each kit contains 12 detergents, supplied as stock solu-tions at 5 or 10 times the reported CMC (Critical Micellar Concentra-tion), with 100 or 200 µl per compound.

The JBScreen Detergents kits are ideal supplements to the JBScreenMembrane screens. This combination will enable you to screen a broadrange of crystallization conditions, while concentrating on the most suc-cessful detergents – and therefore making crystallization screening ofmembrane proteins much more effi cient and less time and sample con-suming.

☛ JBSolution Detergent Test Kit

The JBSolution Detergent Test Kit is designed to optimize solubiliza-tion of membrane proteins. Compounds assembled in the kit range fromionic and nonionic to zwitter-ionic detergents. These detergents havenon-denaturing as well as denaturing properties. The arrangement isbased on years of experience. The Kit contains 4 ml stock solutions of 27detergents with a concentration of 4% and 3 buffers at 1 M concentration(Tris-HCl, HEPES Sodium Salt, NaPB), each at two different pH-values(7.5 and 8.0).


DK-101 JBSolution Detergent Test Kit 1 Kit


CS-501 JBScreen Plus Kosmotropic 1 Kit

CS-502 JBScreen Plus Chaotropic 1 Kit

CS-503 JBScreen Plus Salts 1 Kit

CS-504 JBScreen Plus Additives 1 Kit

CS-505 JBScreen Plus Volatiles 1 Kit

CS-506 JBScreen Plus Complete all 5JBScreen Plus kits for a special price 5 Kits


CD-101 JBScreen Detergents 1 1 Kit

CD-102 JBScreen Detergents 2 1 Kit


X-RAY CRYSTALLOGRAPHYHeavy Atom DerivatizationHeavy Atom Derivatization for Crystallography

☛ JBScreen Heavy

The search for suitable heavy-atom derivatives is often a tedious pro-cess and usually requires screening of a broad range of heavy atoms. TheJBScreen Heavy kit will shorten this process: It contains a collection of24 of the most successful heavy-atom compounds, selected from datamining of heavy-atom derivatized protein crystals, which have beensuccessfully employed in structure determination of biological macro-molecules. Each of the 24 heavy-atom compounds is supplied in threeidentical solid aliquots.


CH-101 JBScreen Heavy 1 Kit

☛ Halogenated nucleotides as rational phasingtools for protein crystallography

For solution of the macromolecular phase problem, the most commonlyused methods (multiple isomorphous replacement (MIR) and that of mul-tiple wavelength anomalous dispersion (MAD)) still involve the incorpora-tion of heavy atoms into protein crystals. After crystallization, findingsuch derivatives is the second major bottle neck in the determination ofthe 3D structure of bio-macromolecules. Most labeling procedures focuson the protein itself in a “trial and error” fashion. Halogenated ATP andGTP analogs however, provide an alternative method that allows rationalincorporation of heavy atoms into a large number of physiologically rel-evant enzymes:

• In the human genome alone, estimated 5,000 to 10,000proteins interact with ATP or GTP.

• The incorporation of iodine or bromine allows MIR or MADphasing for proteins with molecular weights of at least up to50 kDa. Importantly, for MIR experiments, such derivativesare likely to be isomorphous with the native crystal.

• The kinetics of binding of 2’-halogenated ATP analogs tomost enzymes so far investigated.

☛ JBS Halo Kits

Since it is difficult to predict a priori which one of the halogenatednucleotides will give the highest quality co-crystals, with this kit you canscreen them all in parallel.

☛ JBS Halo-ATP Kits

The kit contains all 12 halogenated Adenosine nucleotides (50 units* aslyophilized Na-salts)

• 2’-Iodo-ADP, 2’-Iodo-ATP, 2’-Iodo-AppNHp (2’-Iodo-AMPPNP)

• 2’-Bromo-ADP, 2’-Bromo-ATP, 2’-Bromo-AppNHp (2’-Bromo-AMPPNP)

• 8-Iodo-ADP, 8-Iodo-ATP, 8-Iodo-AppNHp (8-Iodo-AMPPNP)

• 8-Bromo-ADP, 8-Bromo-ATP, 8-Bromo-AppNHp (8-Bromo-AMPPNP)

☛ JBS Halo-GTP Kits

The Kit contains all 6 halogenated Guanosine nucleotides (50 units* aslyophilized Na-salts)

• 8-Iodo-GDP, 8-Iodo-GTP, 8-Iodo-GppNHp (8-Iodo-GMPPNP)• 8-Bromo-GDP, 8-Bromo-GTP, 8-Bromo-GppNHp (8-Bromo-

GMPPNP)


PK-101 JBS Halo-ATP Kit 1 Kit

PK-103 JBS Halo-GTP Kit 1 Kit

☛ Halogen-containing ATP-analogs

2’I-ADP2’-Iodo-adenosine-5’-diphosphate, Sodium salt2’Br-ADP2’-Bromo-adenosine-5’-diphosphate, Sodium salt8I-ADP8-Iodo-adenosine-5’-diphosphate, Sodium salt8Br-ADP8-Bromo-adenosine-5’-diphosphate, Sodium salt2’I-ATP2’-Iodo-adenosine-5’-triphosphate, Sodium salt2’Br-ATP2’-Bromo-adenosine-5’-triphosphate, Sodium salt8I-ATP8-Iodo-adenosine-5’-triphosphate, Sodium salt8Br-ATP8-Bromo-adenosine-5’-triphosphate, Sodium salt2’I-AppNHp (2’I-AMPPNP)2’-Iodo-adenosine-5’-[(β,γ)-imido]triphosphate, Sodium salt2’Br-AppNHp (2’Br-AMPPNP)2’-Bromo-adenosine-5’-[(β,γ)-imido]triphosphate, Sodium salt8I-AppNHp (8I-AMPPNP)8-Iodo-adenosine-5’-[(β,γ)-imido]triphosphate, Sodium salt8Br-AppNHp (8Br-AMPPNP)8-Bromo-adenosine-5’-[(β,γ)-imido]triphosphate, Sodium salt

☛ Halogen-containing GTP-analogs

8I-GDP8-Iodo-guanosine-5’-diphosphate, Sodium salt8Br-GDP8-Bromo-guanosine-5’-diphosphate, Sodium salt8I-GTP8-Iodo-guanosine-5’-triphosphate, Sodium salt8Br-GTP8-Bromo-guanosine-5’-triphosphate, Sodium salt8I-GppNHp (8I-GMPPNP)8-Iodo-guanosine-5’-[(β,γ)-imido]triphosphate, Sodium salt8Br-GppNHp (8Br-GMPPNP)8-Bromo-guanosine-5’-[(β,γ)-imido]triphosphate, Sodium salt

☛ Other halogen-containing nucleotide analogs

8Br-dATP8-Bromo-2’-deoxy-adenosine-5’-triphosphate, Sodium salt8Br-cAMP8-Bromo-Adenosine-3’,5’-cyclic monophosphate, Sodium salt5I-UTP5-Iodo-uridine-5’-triphosphate, Sodium salt5I-dUTP5-Iodo-2’-deoxy-uridine-5’-triphosphate, Sodium salt5Br-UTP5-Bromo-uridine-5’-triphosphate, Sodium salt5Br-dUDP5-Bromo-2’-deoxy-uridine-5’-diphosphate, Sodium salt5Br-dUTP5-Bromo-2’-deoxy-uridine-5’-triphosphate, Sodium salt5I-dCTP5-Iodo-2’-deoxy-cytidine-5’-triphosphate, Sodium salt

☛ Halogenated oligonucleotides

Jena Bioscience is able to synthesize highly pure oligonucleotides con-taining halogenated bases. Please inquire.


Crystallization Plates, Cover Slides, Tape and GreaseX-RAY CRYSTALLOGRAPHY

Consumables

☛ Crystallization plates

☛ Linbro plates

The standard 24-well Linbro plate for hanging drop crystallizations.

☛ Clover Plates by Emerald BioStructures

☛ First Generation CombiClover™ Plates

• Central reservoir is connected to four satellite dropchambers via dedicated vapor diffusion channels, forming anovel combinatorial ”crystallization clover”.

• 24 crystallization clovers provide 96 fl at bottom sittingdrop chambers that are totally clear and free of any coldweld or molding ejection pin marks.

• Open design of the combinatorial ”crystallization clover”allows the simultaneous crystallization screening of fourdifferent macromolecule samples, while reducing by 75 % theamount of crystallization reagents, space and laboratoryplastic ware used.

☛ CombiClover™ Junior Plate

• Use less protein and crystallant per experiment.• Cover the bottom of the sitting drop well with as little as 1µl

of solution.• Utilizing the patented sitting drop well design and vapor

diffusion channel.• Central reservoir is connected to four satellite drop

chambers via vapor diffusion channels, forming a novelcombinatorial ”crystallization clover.”

• 24 crystallization clovers provide 96 fl at-bottom sittingdrop chambers allowing for 4 different experiments withone crystallant per clover.

• Well Volume 6 µl, Reservoir volume 500 µl.

☛ First Generation CompactClover™ Plates

• A “compact clover” design creates four separate reservoirpartitions that are each connected to satellite dropchambers via dedicated vapor diffusion channels.

• 24 compact clovers provide 96 fl at bottom sitting dropchambers (30 microliter capacity) that are totally clear andfree of any cold weld or molding ejection pin marks.

• A single compact crystallization plate can accommodate 4times the number of crystallization trials as compared toother conventional plates of similar size.

• Reservoir chambers accommodate 250 µl of crystallant, andextensive testing has shown that crystallization rates arecomparable to other sitting drop devices.

☛ CompactClover™ Junior Plates

• 96- well plate incorporating patented sitting drop welldesign and vapour diffusion channel.

• Use less protein and crystallant per experiment.• Cover the bottom of the sitting drop well with as little as 1µl

of solution• Quick setup with multichannel pipette• Use for MicroBatch setup• Reservoir volume 300µl, well volume 3 µl.


CPL-101S Linbro Plate 10

CPL-101L Linbro Plate 50


CPL-102 CombiClover Plate includes 2 rolls 20of sealing tape, 2” and 4” wide

CPL-104 Clover plate combo pack contains 10 10+10CombiClover™ plates + 10CompactClover™ plates plus 2 rolls ofsealing tape, 2” and 4” wide

CPL-105 CombiClover Junior Plate Junior 16plate includes one roll of 4“ sealing tape

CPL-103 CompactClover Plate includes 2 rolls 20of sealing tape, 2” and 4” wide

CPL-106 CompactClover Junior Plate contains 1610 CombiClover™ plates + 10CompactClover™ plates plus 2 rollsof sealing tape, 2” and 4” wide

☛ Manco™ Crystal Clear Sealing Tape


CTP-101S Manco Crystal ClearSealing Tape, 2" wide 60 m

CTP-101M Manco Crystal ClearSealing Tape, 3" wide 50 m

CTP-101L Manco Crystal ClearSealing Tape, 4" wide 50 m

☛ Bayer Silicon Grease

The ideal sealant for crystallization setups. Chemical and heat resistant.Medium viscosity.

☛ Standard cover slides

Circular cover slides, 22 mm diameter. Not siliconized! For hangingdrop, sitting drop and sandwich drop experiments.

☛ Siliconized cover slides

Circular cover slides, 22 mm diameter, with siliconized surface. Forhanging drop, sitting drop and sandwich drop experiments.


CSL-101S Standard Circular cover slides,22 mm diameter, not siliconized 100

CSL-101L Standard Circular cover slides,22 mm diameter, not siliconized 1000

CSL-102S Circular cover slides, 22 mmdiameter, siliconized 100

CSL-102L Circular cover slides,22 mm diameter, siliconized 1000


CGR-101 Bayer Silicone Grease 1 Tube (35 g)


X-RAY CRYSTALLOGRAPHYCryo CrystallizationCryo Crystallization

☛ JBScreen Cryo

JBScreen Cryo is a crystallization screen for proteins, peptides, nucleicacids and macromolecular complexes. It contains preformulated reagentssuitable for screening cryo and crystallization conditions using just asingle screen. Based on an extensive data base search [1], the mostsuccessful crystallization conditions employing sufficiently large concen-trations of cryoprotectants and well suited buffers were chosen for theJBScreen Cryo kits.

JBScreen Cryo has been designed for standard vapor diffusion setupsto determine initial crystallization conditions. Crystals grown usingJBScreen Cryo can be directly flash-cooled in liquid nitrogen. If crystalswere grown in non-cryo conditions, simply transfer them into a similarcomposed solution of JBScreen Cryo and allow them to soak for a fewseconds before flash-cooling them.


CC-103 JBScreen Cryo 1 1 Kit




CC-107 JBScreen Cryo 1 - 4 4 Kits

CC-201S JBScreen Cryo HTS S(1.0 ml per well) 1 Block

CC-201L JBScreen Cryo HTS L(1.7 ml per well) 1 Block

☛ JBScreen Cryo Pro

JBScreen Cryo Pro is the most convenient tool on the market for pro-ducing effective cryoprotectants from your crystallization reservoirsolution. The kit contains 12 different compounds, divided into sugar/amino acid-based cryoprotectants, alcohol-based cryoprotectants, andan oil-based cryoprotectant.

☛ Emerald Biosystems

☛☛☛☛☛ Cryo I + II

Sparse matrices for the crystallization of biological macromolecules.Every formulation will flash-freeze to a clear amorphous glass in liquidnitrogen or in the cryo-stream at 100 K. Eleven different cryocrystallantsand sparing use of glycerol ensures a broad sampling of possible cryoconditions. Crystals can be frozen directly from their growth chambers,thus avoiding the additional step of pre-equilibration with an artificialcryo solvent that can damage the crystal.


EBS-CRYO-I Cryo I 1 Kit

EBS-CRYO-II Cryo II 1 Kit

EBS-CRYO-F Cryo I + II 1 + 1 Kit

EBS-BCY Cryo I + II96 well Matrix Block 1 kit


CC-102 JBScreen Cryo Pro 1 Kit


Substrates, Reagents and Assay Kits for Analyzing MMPsMMPs

Enzyme Detection Reagents and Assay kits

☛ Chromogenic/fluorogenic substrates andantibodies for matrix metalloproteinases (MMPs)

Protease Chromogenic/ Fluorogenic Antibodies Assay KitsSubstrates

MMP-1 27082, 27084, 27086, 27088,60571, 60572, 60576, 60578, 29574 71128, 7115060579, 60581, 60582

MMP-2 27076, 27090, 27094, 27096,60569, 60570, 60571, 60572, 29575 71129, 7115160576, 60578, 60579, 60581,60582

MMP-3 27098, 27100, 60579, 60580, 29576 71130, 7115260581

MMP-7 27076, 27102, 27104 29577 71132, 71153

MMP-8 27106 29578 71133, 71154

MMP-9 27088, 27094, 27096, 60570, 29579 71134, 7115560581

MMP-12 60569, 60570, 60571, 60572,60574, 60575, 60576, 60578, 71137, 7115760579, 60580, 60581, 60582

MMP-13 27108 71135, 71156

☛ EnzoLyte™ 490 MMP-1 Assay Kit *Fluorimetric*

The EnzoLyte™ 490 MMP-1 Assay Kit uses an optimized fluorogenicpeptide substrate for continuous measurement of MMP-1 activities.


71128 EnzoLyte™ 490 MMP-1 Assay Kit 500*Fluorimetric* assays

Contents:EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nmupon cleavage), Assay buffer, Fluorescence reference standard forcalibration, A detailed protocol


EnzoLyte™ 520 MMP-1 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the MMP-1 activities. Upon the cleavage of the FRET peptide byMMP-1, the fluorescence of 5-FAM is recovered, and can be continuouslymonitored at excitation/emission = 490 nm/520 nm.



Contents:5-FAM/QXL520™ FRET Peptide substrate for MMP-1, Assay buffer,Fluorescence reference standard for calibration, A detailed protocol


The EnzoLyte™ 490 MMP-2 Assay Kit is a complete assay system designedto continuously analyze MMP activities or to screen MMP-2 inhibitors.



EnzoLyte™ 520 MMP-2 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of MMP-2 activities. Upon the cleavage of the FRET peptide byMMP-2, the fluorescence of 5-FAM is recovered, and can be continuouslymonitored at excitation/emission = 490 nm/520 nm.

Contents:

5-FAM/QXL520™ FRET Peptide substrate for MMP-2, Assay buffer,Fluorescence reference standard for calibration, A detailed protocol.

☛☛☛☛☛ EnzoLyte™ 490 MMP-3 Assay Kit *Fluorimetric*

The EnzoLyte™ 490 MMP-3 Assay Kit is a complete assay system designedto continuously analyze MMP activities or to screen MMP-3 inhibitorsusing a fluorogenic MMP-3 substrate.







EnzoLyte™ 520 MMP-3 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the enzyme activities. This substrate showed excellent specific-ity to MMP-3 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13and 14. Upon the cleavage of the FRET peptide by MMP-3, the fluores-cence of 5-FAM is recovered, and can be continuously monitored atexcitation/emission = 490 nm/520 nm.



Contents:5-FAM/QXL520™ FRET Peptide substrate for MMP-3, Assay buffer,Fluorescence reference, standard for calibration, A detailed protocol


The EnzoLyte™ 490 MMP-7 Assay Kit is a complete assay system designedto continuously analyze MMP activities or to screen MMP-7 inhibitors byusing a fluorogenic MMP-7 substrate. The assays are performed in aconvenient 96-well or 384-well microplate format.







Assay Kits for Analyzing MMPsMMPs


� EnzoLyte™ 490 MMP-12 Assay Kit *Fluorimetric*

The EnzoLyte™ 490 MMP-12 Assay Kit is a complete assay system designedto continuously analyze MMP-12 activities or to screen for MMP-12 in-hibitors by using a fluorogenic MMP-12 substrate.


EnzoLyte™ 520 MMP-7 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the enzyme activities. In an intact FRET peptide, the fluores-cence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRETpeptide by MMP-7, the fluorescence of 5-FAM is recovered, and can becontinuously monitored at excitation/emission = 490 nm/520 nm.



Contents:5-FAM/QXL520™ FRET Peptide substrate for MMP-7, Assay buffer,Fluorescence reference, standard for calibration


EnzoLyte™ 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the enzyme activities. In an intact FRET peptide, the fluores-cence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRETpeptide by MMP-8, the fluorescence of 5-FAM is recovered, and can becontinuously monitored at excitation/emission = 490 nm/520 nm.


71154 EnzoLyte™ 520 MMP-8 Assay Kit 100 assays*Fluorimetric*

Contents:5-FAM/QXL520™ FRET Peptide substrate for MMP-8, AssaybufferFluorescence reference standard for calibration


The EnzoLyte™ 490 MMP-9 Assay Kit is a complete assay system designedto continuously analyze MMP activities or to screen for MMP-9 inhibitorsusing a fluorogenic MMP-9 substrate.





EnzoLyte™ 520 MMP-9 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the enzyme activities. In an intact FRETpeptide, the fluores-cence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRETpeptide by MMP-9, the fluorescence of 5-FAM is recovered, and can becontinuously monitored at excitation/emission = 490 nm/520 nm.






EnzoLyte™ 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the enzyme activities. This substrate showed excellent specific-ity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenchedby QXL520™. Upon the cleavage of the FRET peptide by MMP-12, thefluorescence of 5-FAM is recovered, and can be continuously monitoredat excitation/emission = 490 nm/520 nm.







The EnzoLyte™ 490 MMP-13 Assay Kit is a complete assay system designedto continuously analyze MMP-13 activities or to screen MMP-13 inhibi-tors by using a fluorogenic MMP-13 substrate.





EnzoLyte™ 520 MMP-13 Assay Kit uses a 5-FAM (fluorophore) and QXL520™(quencher) labeled FRET peptide substrates for continuous measure-ment of the enzyme activities. This substrate showed excellent specific-ity to MMP-13 with minimal cross-reaction with MMP-1, 2, 3, 7, 8, 9, 12,and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenchedby QXL520™. Upon the cleavage of the FRET peptide by MMP-13, thefluorescence of 5-FAM is recovered, and can be continuously monitoredat excitation/emission = 490 nm/520 nm.





☛ Substrates for MMPs


85111 Collagen (Type I), FITC conjugated 1 mg*Water Soluble* for MMP-1

85102 Collagen (Type I), FAM conjugated *Water 5 mgInsoluble* for MMP-1, slow digesting & specific

85112 Collagen (Type IV), FAM conjugated for MMP-2, 1 mgother Geloatinases & Collagenases.

85113 Elastin, FAM Conjugated 1 mgFor elastase & other proteases

85103 Elastin, FAM Conjugated *Water Insoluble*, 1 mgslow digesting & specific

85145 Gelatin, FAM Conjugated 5 mgFor gelatinases & collagenases

27078 MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-OH 1 mgGeneric substrate for all MMPs

27074 DNP-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 1 mgGeneric substrate for all MMPs

27082 MMP-1 Substrate I 1 mg[DNP-Pro-Leu-Ala-Leu-Trp-Ala-Arg-OH]

27084 MMP-1 Substrate II 1 mg[DNP-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg-NH2]

27086 MMP-1 Substrate III 1 mg[DNP-Pro-Cha-Abu-Cys(Me)-His-Ala-Lys(Abz(N-Me))-NH2]

27088 MMP-1/MMP-9 Substrate 1 mg[DNP-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Abz(N-Me))-NH2]

27090 MMP-7/8/13 Substrate I 1 mg[MCA-Pro-Leu-Ala-Nva-Dpa-Ala-Arg-NH2]

27076 MMP-7/8/13 Substrate II 1 mg[MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2]

27094 MMP-2/MMP-9 Substrate I 1 mg[DNP-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH]

27096 MMP-2/MMP-9 Substrate II 1 mg[Ac-Pro-Leu-Gly-Mmp-Leu-Gly-OC

2H

5]

27098 MMP-3 Substrate I 1 mg[DNP-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-OH]

27100 MMP-3 Substrate II 1 mg[NBD-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(DMC)-NH

2] Can also be an effective substrate

for MMP-1, MMP-7, MMP-12 and MMP-13.

27102 MMP-7 Substrate I 1 mg[DNP-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser-OH]

27104 MMP-7 Substrate II 1 mg[DABCYL-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser-EDANS]

27106 MMP-8 Substrate 1 mg[DNP-Pro-Leu-Ala-Tyr-Trp-Ala-Arg-OH]

27108 MMP-13 Substrate 1 mg[MCA-Pro-Cha-Gly-Nva-His-Ala-Dpa- NH

2]

Inhibited by the tissue inhibitors of 1 mgmetalloproteinases, TIMP-1, TIMP-2 and TIMP-3.

27110 NFF-2 1 mg[MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH

2]

27114 NFF-3 1 mg[MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH

2]

27061 TNO113 1 mg[DABCYL-GABA-Pro-Cha-Abu-Smc-His-Ala-Glu(EDANS)-Ala-Lys-NH

2]

25350 TNO211 1 mg[DABCYL-g-Abu -Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH2]

☛ FRET based MMP substrates

These are based on the FAM/QXL™ 520 FRET.

Cat. No. Product MMP recognized Qty

60579-01 5-FAM-Arg-Pro-Lys-Pro-Tyr- MMP-1, 2, 12, 0.1 mgAla-Nva-Trp-Met-Lys(QXLTM and 13520)-NH2

60580-01 5-FAM-Arg-Pro-Lys-Pro-Val- MMP-3, MMP-12 0.1 mgGlu-Nva-Trp-Arg-Lys(QXLTM 520)-NH2

60578-01 5-FAM-Pro-Cha-Gly-Nva-His-Ala-Dap(QXLTM 520)-NH2 MMP-3, MMP-12 0.1 mg

60571-01 5-FAM-Pro-Leu-Ala-Nva-Dap MMP-1, 2, 7, 8,(QXL TM 520)-Ala-Arg-NH2 12, and 13 0.1 mg

60572-01 5-FAM-Pro-Leu-Gly-Leu-Dap(QXL™ 520)-Ala-Arg-NH2 MMP-3, MMP-12 0.1 mg

60583-01 QXL™ 520-Arg-Pro-Lys- MMP-12, MMP-13 0.1 mgPro-Gln-Gln-Phe-Trp-Lys(5-FAM)-NH

2

60581-01 QXL™ 520-?-Abu-Pro-Cha- MMP-1, 2, 3, 7, 8, 0.1 mgAbu-Smc-His-Ala-Dab 9, 12 and 13(5-FAM)-Ala-Lys-NH

2

(Smc=S-Methyl-L-cysteine)

60575-01 QXL™ 520-Arg-Pro-Lys-Pro- MMP-7, MMP-12,Leu-Ala-Nva-Trp-Lys MMP-13 0.1 mg(5-FAM)-NH

2

60569-01 QXL™ 520-Pro-Leu-Ala-Leu- MMP-1, 7, 8, 12,Trp-Ala-Arg-Lys(5-FAM)-NH

2and 13 0.1 mg

60577-01 QXL™ 520 -Pro-Leu-Ala-Tyr- MMP-13 0.1 mgTrp-Ala-Arg-Lys(5-FAM)-NH

2

60570-01 QXL™ 520-Pro-Leu-Gly-Cys MMP-1, 2, 8, 9, 0.1 mg(Me)-His-Ala-D-Arg-Lys 12, and 13(5-FAM)-NH

2

60568-01 QXL ™ 520-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-Lys(5-FAM)-NH

2MMP-13 0.1 mg

60573-01 QXL™ 520-Pro-Leu-Gly-Met-Trp-Ser-Arg-Lys(5-FAM)-NH

2MMP-2, MMP-13 0.1 mg

60574-01 QXL™ 520-Pro-Tyr-Ala-Tyr- MMP-7, MMP-12,Trp-Met-Arg-Lys(5-FAM)-NH

2MMP-13 0.1 mg

60582-01 QXL™ 520-?-Abu-Pro-Gln-Gly- MMP-1, 2, 7, 8,Leu-Dab(5-FAM)-Ala-Lys-NH

212 and 13 0.1 mg

60576-01 QXL™ 520-Arg-Pro-Leu-Ala- MMP-1, 2, 7, 8,Leu-Trp-Arg-Lys(5-FAM)-NH

212 and 13 0.1 mg

60585-01 QXL™ 570-Lys-Pro-Leu-Ala- Generic MMPNva-Dap(5-TAMRA)-Ala-Arg-NH

2substrate 0.1 mg

☛ MMP Antibodies

Western blot and immunohistochemistry


29574 Anti-MMP 1 0.1 mg

29575 Anti-MMP 2 0.1 mg

29576 Anti-MMP 3 0.1 mg

29577 Anti-MMP 7 0.1 mg

29578 Anti-MMP 8 0.1 mg

29579 Anti-MMP 9 0.1 mg

29580 Anti-MMP 10 0.1 mg

29581 Anti-MMP 16 0.1 mg

Chromogenic & Flurogenic Substrates for Detecting MMPs MMPsEnzyme Detection Reagents and Assay kits


Reagents and Assay Kits for Analyzing Viral ProteasesVIRAL PROTEASES


☛ EnzoLyte™ 490 HCV Protease Assay Kit*Fluorimetric*

The EnzoLyte™ 490 HCV Protease Assay Kit uses an optimized FRET peptidesubstrate for the continuous measurement of HCV NS3/4a activity. ThisFRET substrate is cleaved specifically by HCV NS3/4a protease therebyliberating the C-terminal peptide-fluorophore fragment from theproximity quenching effect of the dark quencher, resulting in a morethan 10-fold increase in fluorescence.

Contents:EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nmupon cleavage), Assay buffer, Fluorescence reference standard forcalibration

☛☛☛☛☛ EnzoLyte™ 520 HCV Protease Assay Kit*Fluorimetric*

The EnzoLyte™ 520 HCV Assay Kit uses a new FRET peptide substrate thatincorporates 5-FAM (fluorophore) and QXL™ 520 (quencher) for a con-tinuous measurement of enzyme activities. In the intact FRET peptide,the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of theFRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM isrecovered and can be continuously monitored at excitation/emission =490 nm/520 nm.


71126 EnzoLyte™ 490 HCV Protease Assay Kit 500*Fluorimetric* assays


71145 EnzoLyte™ 520 HCV Protease Assay Kit 100*Fluorimetric* assays

Contents:5-FAM/QXL™ 520-based FRET peptide substrate (Ex/Em=490/520 nm uponcleavage), Fluorescence reference standard for calibration

☛ EnzoLyte™ 620 HCV Protease Assay Kit *Fluorimet-

ric*

The EnzoLyte™ 620 HCV Assay Kit uses a new FRET peptide substrate thatincorporates HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher)for a continuous measurement of enzyme activities. In the intact FRETpeptide, the fluorescence of Hilyte Fluor™ TR is quenched by QXL™ 610.Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluores-cence of Hilyte Fluor™ TR is recovered and can be continuously moni-tored at excitation/emission = 590 nm/620 nm.


71146 EnzoLyte™ 620 HCV Protease Assay 100Kit *Fluorimetric* assays

Contents:HiLyte Fluor™ TR/QXL™ 610-based FRET peptide substrate (Ex/Em=590/620 nm upon cleavage), Assay buffer, Fluorescence referencestandard for calibration

☛ HCV Protease FRET Substrate IThis is a HCV protease substrate incorporating an ester bond betweenresidues P1 and P1’. It is widely used for continuous assay of NS3 proteaseactivity.


22991-025 HCV Protease FRET Substrate I 0.25 mg[Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH

2]

This is an internally quenched fluorescent substrate cleaved specificallybetween the Ala-Ser peptide bond thereby liberating the C-terminal peptide-EDANS fragment from the proximity quenching effect of DABCYL group.


22998 CMV Protease FRET Substrate I 1 mg[DABCYL-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-EDANS]

☛☛☛☛☛ EnzoLyte™ 490 HIV Protease Assay Kit*Fluorimetric*

The EnzoLyte™ 490 HIV Protease Assay Kit uses an optimized FRET pep-tide substrate for a continuous measurement of HIV protease activities.This FRET-based fluorogenic substrate is derived from a natural pro-cessing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR withthis substrate results in specific cleavage and a time-dependent increasein fluorescence intensity that is linearly related to the extent of sub-strate hydrolysis.


71127 EnzoLyte™ 490 HIV Protease Assay 500Kit *Fluorimetric* assays

Contents:EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nmupon cleavage), Assay buffer, HIV protease inhibitor, Fluorescencereference standard for calibration, A detailed protocol

☛☛☛☛☛ EnzoLyte™ 520 HIV Protease Assay Kit*Fluorimetric*

The EnzoLyte™ 520 HIV Assay Kit uses a new FRET peptide substrate thatincorporates 5-FAM (fluorophore) and QXL™ 520 (quencher) for continu-ous measurement of enzyme activities. In the intact FRET peptide, thefluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of theFRET peptide by HIV protease, the fluorescence of 5-FAM is recoveredand can be continuously monitored at excitation/emission = 490 nm/520 nm.


71147 EnzoLyte™ 520 HIV Protease Assay Kit 100*Fluorimetric* assays

Contents:5-FAM/QXL™ 520-based FRET peptide substrate (Ex/Em=490/520 nmupon cleavage), Assay buffer, HIV protease inhibitor, Fluorescencereference standard for calibration, A detailed protocol

☛ EnzoLyte™ 620 HIV Protease Assay Kit*Fluorimetric*

EnzoLyte™ 620 HCV Assay Kit uses a new FRET peptide substrate thatincorporates HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher)for continuous measurement of the enzyme activities. In the intact FRETpeptide, the fluorescence of Hylite Fluor™ TR is quenched by QXL™ 610.Upon cleavage of the FRET peptide by HIV protease, the fluorescence ofHylite Fluor™ TR is recovered and can be continuously monitored atexcitation/emission = 590 nm/620 nm.


71148 EnzoLyte™ 620 HIV Protease Assay Kit 100*Fluorimetric* assays

Contents:FRET peptide substrate (fluorogenic indicator, Ex/Em=590/610nm uponcleavage), Assay buffer, HIV protease inhibitor, Fluorescencereference standard for calibration, A detailed protocol

☛ HIV Protease FRET Substrate I

Incubation of recombinant HIV-1 PR with the fluorogenic substrate re-sulted in specific cleavage at the Tyr-Pro bond and a time-dependentincrease in fluorescence intensity that is linearly related to the extent ofsubstrate hydrolysis. The fluorescence quantum yields of the HIV-1 PRsubstrate in the FRET assay increased by 40.0- and 34.4-fold, respec-tively, per mole of substrate cleaved.


22992 HIV Protease FRET Substrate I 1 mg


☛ EnzoLyte™ Green Protease Assay Kit*Fluorimetric*

Our EnzoLyte™ Green Protease Assay Kit is widely used for detection ofgeneric protease activities. The kit uses a casein derivative that isheavily labeled with a rhodamine derivative, resulting in almost totalquenching of the conjugate’s fluorescence. Protease-catalyzed hydroly-sis relieves this quenching conjugate, yielding brightly green fluorescentdye–labeled peptides. The EnzoLyte™ protease assay kits do not requireany separation steps and can be used to continuously measure the kinet-ics of a variety of exopeptidases and endopeptidases.


71124 EnzoLyte™ Green Protease Assay 500 assaysKit *Fluorimetric*

Contents:Fluorescently labeled casein with high ratio of dye/protein, Trypsin(positive control), Assay buffer, A detailed protocol

☛☛☛☛☛ EnzoLyte™ Red Protease Assay Kit *Fluorimetric*

Same as above, except that it uses Rhodamine.


71140 EnzoLyte™ Red Protease Assay Kit 500*Fluorimetric* assays

Contents:Fluorescently labeled casein with high ratio of dye/protein (Ex/Em=546/575nm on cleavage), Trypsin (positive control), Assay buffer

☛ Substrates for Proteases

☛☛☛☛☛ Casein, FITC/TAMRA Conjugated


85100 Casein, FITC Conjugated 5 mg

85101 Casein, TAMRA Conjugated 5 mg

☛☛☛☛☛ Substrate for Trypsin/Cathepsin B & L

The colorless (Z-Arg) 2Rh110 is hydrolyzed to highly fluorescent rhodamine110.


60323-5 (Z-Arg)2Rh110 5 mg[Rhodamine 110, bis-(CBZ-L-arginine amide)]

☛☛☛☛☛ Renin FRET Substrate I

This substrate contains a Leu-Val renin cleavage site that occurs in theN-terminal peptide of human angiotensinogen. Cleavage of the substrateby renin liberates the peptidyl-EDANS fragment from proximity with theDABCYL acceptor, restoring the fluorescence of the EDANS moiety.


24478 Renin FRET Substrate I 1 mg(DABCYL-g-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS)

☛ TACE FRET Substrate I

TACE FRET substrate I is derived from the cleavage sequence of pro-TNFa recognized by TNFa-cleaving enzyme (TACE). Incubation of theTACE substrate with recombinant human TACE gives a specific cleavageto restore the quenched fluorescence. The substrate is widely used toscreen inhibitors of TNF-convertase.


25043 TACE FRET Substrate I 1 mg(DABCYL-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS)

☛ Substrate for Calpain

The colorless and nonfluorescent (Z-Ala-Ala) 2Rh110 is hydrolyzed tohighly fluorescent rhodamine 110. Alternatively, (Z-Ala-Ala) 2Rh110 canalso be used to detect calpain in a chromogenic mode since the enzymaticproduct (rhodamine 110) exhibits a large extinction coefficient.


60320-5 (Z-Ala-Ala)2Rh110 5 mg[Rhodamine 110, bis-(CBZ-L-alanyl-L-alanine amide)]

☛ Substrate for Elastase

The colorless and nonfluorescent (Z-Ala-Ala-Ala-Ala) 2Rh110 is hydro-lyzed to highly fluorescent rhodamine 110.


60321-5 (Z-Ala-Ala-Ala-Ala) 2Rh110 5 mg[Rhodamine 110, bis-(CBZ-L-alanyl-L-alanyl-L-alanyl-L- alanine amide)]

☛ Substrate for Elastase/Trypsin

The colorless and nonfluorescent (Z-Ala-Arg) 2Rh110 is hydrolyzed tohighly fluorescent rhodamine 110.


60322-5 (Z-Ala-Arg)2Rh110 5 mg[Rhodamine 110, bis-(CBZ-L-alanyl-L-arginine amide)]

☛ Malaria Aspartyl Proteinase FRET Substrate I

A useful fluorogenic substrate for the continuous assay of malaria aspar-tyl proteinase.


24480 Malaria Aspartyl Proteinase FRET 1 mgSubstrate I [DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS]

Reagents and Assay Kits for Analyzing Miscellaneous ProteasesPROTEASES




85624 5-Bromo-4-chloro-3-indoxyl-N-acetyl 5 mg-α-D-N-acetylneuraminic acid, sodium saltChromogenic neuraminidase substrateused in histochemistryλ

abs: ~610 nm; λ

em: N/D

85601 CUG 10 mg[3-Carboxyumbelliferyl-β-D-galactopyranoside]Excellent fluorogenic substrate for detectingβ-galactosidaseλ

abs: 386 nm; λ

em: 448 nm

85602 FDGlcU 5 mg[Fluorescein di-β-D-glucuronide]The most sensitive fluorogenic substratefor detecting β-glucuronidaseλ

abs: 490 nm; λ

em: 514 nm

85607 4-Methylumbelliferyl-β-D-glucoside 250 mgExcellent fluorogenic substrate fordetecting ß-glucosidaseλ

abs: 360 nm; λ

em: 499 nm

85605 MUG 250 mg[4-Methylumbelliferyl-β-D-galactopyranoside]Excellent fluorogenic substrate fordetecting β-galactosidaseλ

abs: 360 nm; λ

em: 499 nm

85606 MUGlcU 100 mg[4-Methylumbelliferyl-β-D-glucuronide]Excellent fluorogenic substrate fordetecting β-galactosidaseλ

abs: 360 nm; λ

em: 499 nm

85618 4-Nitrophenyl-N-acetyl-b-D-galactosaminide 100 mgChromogenic N-acetylgalactosaminidasesubstrate, λ

abs: 399 nm

85619 4-Nitrophenyl-N-acetyl-b-D-glucosaminide 100 mgChromogenic N-acetylglucosaminidasesubstrate, λ

abs: 399 nm; λ

em: N/A

85634 4-Nitrophenyl-β-D-cellobioside 100 mgChromogenic cellobiohydralase(e.g. cellobiosidase) substrate usedin histochemistryλ

abs: 399 nm; λ

em: N/A

85637 4-Nitrophenyl-β-D-fucopyranoside 100 mgChromogenic substrate for β-fucosidaseused in histochemistryλ

abs: 399 nm; λ

em: N/A

85638 4-Nitrophenyl-α-D-galactopyranoside 1 gExcellent chromogenic α-galactosidase substrateλ

abs: 399 nm; λ

em: N/A

85636 4-Nitrophenyl-β-D-galactopyranoside 1 gExcellent chromogenic α-galactosidase substrateλ

abs: 399 nm; λ

em: N/A

85639 4-Nitrophenyl-α-D-glucopyranoside 1 gExcellent chromogenic α-galactosidase substrateλ

abs: 399 nm; λ

em: N/A

85629 4-Nitrophenyl-β-D-glucopyranoside 1 gExcellent chromogenic α-galactosidase substrateλ

abs: 399 nm; λ

em: N/A

85635 X-β-xyloside 25 mg[5-Bromo-4-chloro-3-indoxyl-β-D-xylopyranoside] Chromogenic β-xylosidasesubstrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D


85640 4-Nitrophenyl-β-D-glucuronic acid, 100 mgsodium salt chromogenic α-galactosidase substrateλ

abs: 399 nm; λ

em: N/A

85641 4-Nitrophenyl-α-D-mannopyranoside 100 mgchromogenic α-galactosidase substrateλ

abs: 399 nm; λ

em: N/A

85615 β-Trifluoromethylumbelliferyl-β-D- 100 mggalactopyranosidechromogenic α-galactosidase substrateλ

abs: 385 nm; λ

em: 502 nm

85616 β-Trifluoromethylumbelliferyl-β- 25 mgD-glucuronide fluorogenicglucuronidase substrateλ

abs: 385 nm; λ

em: 502 nm

85625 X-cellobioside 100 mg[5-Bromo-4-chloro-3-indoxyl-N-acetyl-β-D-cellobioside] Chromogeniccellobiohydralase (e.g. cellobiosidase)substrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D

85626 X-fucoside 25 mg[5-Bromo-4-chloro-3-indoxyl-β-D-fucopyranoside] Chromogenic substratefor β-fucosidase used in histochemistryλ

abs: ~610 nm; λ

em: N/D

85627 X-a-Gal 25 mg[5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside] Excellentchromogenic α-galactosidasesubstrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D

85620 X-Gal 1 g[5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside]Excellent chromogenic α-galactosidasesubstrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D

85622 X-GalNAc 25 mg[5-Bromo-4-chloro-3-indoxyl-N-acetyl-β-D-galactosaminide]Chromogenic N-acetylgalactosaminidasesubstrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D

85623 X-GluNAc 25 mg[5-Bromo-4-chloro-3-indoxyl-N-acetyl-β-D-glucosaminide] Chromogenic N-acetylgalactosaminidase substrate usedin histochemistryλ

abs: ~610 nm; λ

em: N/D

85630 X-GlcU 25 mg[5-Bromo-4-chloro-3-indoxyl-β-D-glucuronic acid, sodium salt]Excellent chromogenic β-glucuronidasesubstrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D

85628 X-α-Glu 25 mg[5-Bromo-4-chloro-3-indoxyl-α-D-glucopyranoside] Excellent chromogenicα-galactosidase substrate used inhistochemistryλ

abs: ~610 nm; λ

em: N/D

85632 X-α-D-mannoside 100 mg[5-Bromo-4-chloro-3-indoxyl-α-D-mannopyranoside]Chromogenic α-manosidasesubstrate used in histochemistryλ

abs: ~610 nm; λ

em: N/D

Chromogenic & Fluorogenic Substrates for Detecting Glycosidases GLYCOSIDASESEnzyme Detection Reagents and Assay kits


☛☛☛☛☛ EnzoLyte™ FDP Alkaline Phosphatase Assay Kit*Fluorimetric*

The EnzoLyte™ FDP Alkaline Phosphatase Assay Kit uses highly purifiedFDP to quantify alkaline phosphatase activity in solutions, in cell ex-tracts, in live cells as well as on solid surfaces (such as PVDF mem-branes).


71109 EnzoLyte™ FDP Alkaline Phosphatase 500Assay Kit *Fluorimetric* assays

Contents:FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephospho-rylation), Calibration standard, Reaction buffer, Stop buffer.

☛ EnzoLyte™ FDP Alkaline Phosphatase ELISAAssay Kit *Fluorimetric*

The EnzoLyte™ FDP Alkaline Phosphatase ELISA Assay Kit uses highly puri-fied FDP to quantify alkaline phosphatase activity in ELISA.


71101-R EnzoLyte™ FDP Alkaline Phosphatase 500ELISA Assay Kit *Fluorimetric* assays

Contents:FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephospho-rylation), Assay buffer, Stop solution, Wash buffer, Alkaline phosphatase-conjugated secondary antibody (goat anti-rabbit IgG), An optimizedELISA protocol

☛ Fluorescein diphosphate, tetraammonium salt(FDP)

Please view page#

☛ EnzoLyte™ pNPP Alkaline Phosphatase ELISAAssay Kit *Colorimetric*

The EnzoLyte™ pNPP Alkaline Phosphatase ELISA Assay Kit provides opti-mized pNPP assay buffer, modified ELISA wash buffer and AP-conju-gated secondary antibody. The assay is highly sensitive and has a largedynamic range.


71232-M EnzoLyte™ pNPP Alkaline Phosphatase 500ELISA Assay Kit *Colorimetric* assays

☛ EnzoLyte™ pNPP Secreted Alkaline PhosphataseReporter Gene Assay Kit *Colorimetric*

EnzoLyteTM pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kitprovides a sensitive colorimetric assay of placental alkaline phosphatasefor both secreted and membrane-bound form.


71233 EnzoLyte™ pNPP Secreted Alkaline 500Phosphatase Reporter Gene Assay assaysKit *Colorimetric*

Contents:pNPP chromogenic substrate (absorption max = 405 nm on dephosphory-lation), Assay buffer, Stop solution.

☛ Other Chromogenic and Fluorogenic Substratesfor Detecting Phosphatases and Phospholipases


85633 BCIP *UltraPure Grade* 100 mg[5-Bromo-4-chloro-3-indoxylphosphate, disodium salt]λ

abs: ~610 nm; λ

em: N/A

85301 pNPP 1 g[4-Nitrophenyl phosphate,disodium salt] *UltraPure Grade*Excellent chromogenic phosphatasesubstrate, λ

abs: 399 nm

85310 MUP *UltraPure Grade*4- 1 gMethylumbelliferyl phosphate,free acid]Abs (max): 360 nm; λ

em: 499 nm

85311 MUP, DCA *UltraPure Grade*4- 1 gMethylumbelliferyl phosphate,dicyclohexylammonium salt, trihydrate]λ

abs: 360 nm; λ

em: 499 nm

85312 MUP, DSS *UltraPure Grade* 1 g[4-Methylumbelliferyl phosphate,disodium salt] λ

abs: 360 nm;

λem

: 499 nm

85631 X-InP 5 mg[5-Bromo-4-chloro-3-indoxylmyo-inositol-1-phosphate, ammoniumsalt]

☛ FDG [Fluorescein di-βββββ-D-galactopyranoside]

Fluorescein di- β -D-galactopyranoside (FDG) is one of the most sensitivefluorogenic substrates available for detecting β -galactosidase. Galac-tosidase-catalyzed hydrolysis of FDG can be followed by fluorescenceincrease around 520 nm. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry.

☛ Resorufin βββββ -D-Galactopyranoside

Unlike FDG that requires a two-step hydrolysis to generate maximumfluorescence, resorufin β -D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence. Resorufin galacto-side has also been used to quantitate β -galactosidase activity in singleyeast cells by flow cytometry and to detect immobilized β-galactosidaseactivity.


85600 FDG 5 mg[Fluorescein di-β-D-galactopyranoside]

85617 Resorufin β -D-Galactopyranoside 25 mg

Reagents and Assay kits for Phosphatases,Phospholipases and Galactosidases

PHOSPHATASES/PHOSPHOLIPASES/ GALACTOSIDASESEnzyme Detection Reagents and Assay kits


Reagents and Assay Kits for Analyzing Secretasesβββββ-Secretase


☛☛☛☛☛ EnzoLyte™ 520 βββββ-Secretase Assay Kit

EnzoLyte™ β -Secretase Assay Kit provides a sensitive and efficient methodfor screening potential b -secretase inhibitors. This kit uses a β-secretase-cleavable FRET peptide substrate. The specific cleavage of the peptideby β -secretase physically separates the donor and acceptor, recoveringthe quenched fluorescence that is directly related to β -secretase activ-ity.


71144 EnzoLyte™ 520 β -Secretase Assay Kit 100assays

Contents:FRET peptide substrate (fluorogenic indicator, Ex/Em = 488/520 nmupon cleavage), β -Secretase (positive control), Assay buffer, Fluores-cence reference standard for calibration, A detailed protocol

☛ βββββ-Secretase Substrate I

This is β FRET-based α-secretase substrate that is cleaved by β-secretase,thereby liberating the MCA fragment from the proximity quenching effectof the DNP group. This cleavage results in fluorescence enhancement thatis observed at the MCA emission wavelength of ~390 nm.


60271 β-Secretase Substrate I 1 mg[MCA-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Lys(DNP)-OH]

☛ βββββ-Secretase Substrate I

This substrate is cleaved by β-secretase thereby liberating the MCAfragment from the proximity quenching effect of the DNP group. Thisresults in fluorescence enhancement that is observed at the MCA emissionwavelength of ~390 nm.


60268 β-Secretase Substrate I 1 mg[MCA-Glu-Val-Lys-Val-Asp-Ala-Glu-Phe-Lys(DNP)-OH]

☛ βββββ-Secretase Substrate II

This is an internally quenched fluorescent β-secretase substrate wherethe Val residue is replaced by Met. This substrate is cleaved byβ-secretase, thereby liberating the MCA fragment from the proximityquenching effect of the DNP group. This cleavage results in fluorescenceenhancement that is observed at the MCA emission wavelength of~390 nm.


60269 β-Secretase Substrate II 1 mg[MCA-Glu-Val-Lys-Met-Asp-Ala-Glu-Phe-Lys(DNP)-OH]

☛☛☛☛☛ βββββ -Secretase Substrate V

This is a FRET-based β-secretase substrate that is cleaved by β-secretase,thereby liberating the HiLyte Fluor™ 488 fragment from the proximityquenching effect of the QXL™ 520 group. This results in fluorescenceenhancement that is observed at the HiLyte Fluor emission wavelength of~520 nm with excitation at 488 nm.


60604-01 β -Secretase Substrate V 1 mg

[HiLyte Fluor™ 488-Glu-Val-Asn-

Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH]

☛☛☛☛☛ βββββ-Secretase Substrate III

This is a FRET-based b-secretase substrate that is cleaved by β -secretase,thereby liberating the MCA fragment from the proximity quenching ef-fect of the DNP group. This cleavage results in fluorescence enhancementthat is observed at the MCA emission wavelength of ~390 nm.


60270 β -Secretase Substrate III 1 mg[MCA-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DNP)-OH]


Reagents & Assay kits for Peroxidases, Oxidases & Dehydrogenases PEROXIDASES/REDOX ENZYMESEnzyme Detection Reagents and Assay kits

☛ ADHP (10-Acetyl-3,7-dihydroxyphenoxazine)

This is not only a stable fluorogenic substrate for HRP but also anultrasensitive probe for H2O2. In the presence of HRP and H

2O

2, ADHP

generates highly fluorescent resorufin that has maximum absorption of571 nm and maximum emission of 585 nm.


85500 ADHP 25 mg(10-Acetyl-3,7-dihydroxyphenoxazine)

☛☛☛☛☛ EnzoLyte™ ADHP Hydrogen Peroxide Assay Kit*Fluorimetric*

EnzoLyte™ ADHP Hydrogen Peroxide Assay Kit uses highly purified ADHPto quantify hydrogen peroxide in solutions, in cell extracts and in livecells. It can also be used to detect a variety of oxidase activities throughenzyme-coupled reactions.


71112 EnzoLyte™ ADHP Hydrogen Peroxide 500Assay Kit *Fluorimetric* assays

☛ EnzoLyte™ ADHP Peroxidase Assay Kit*Fluorimetric*

EnzoLyte™ ADHP Peroxidase Assay Kit uses highly purified ADHP to quan-tify peroxidase activity in solutions, in cell extracts and in live cells andon solid surfaces (such as PVDF membranes).


71111 EnzoLyte™ ADHP Peroxidase Assay 500Kit *Fluorimetric* assays

☛ Entoleted™ ADHP Peroxidase ELISA Assay Kit*Fluorimetric*

EnzoLyte™ ADHP Peroxidase ELISA Assay Kit uses highly purified ADHP toquantify HRP activity in the secondary antibody or streptavidin-HRPconjugates.


71110-R Entoleted™ ADHP Peroxidase 500ELISA Assay Kit *Fluorimetric* assays

☛ EnzoLyte™ ADHP Peroxidase ELISA Assay Kit*Fluorimetric*

EnzoLyte™ ADHP Peroxidase ELISA Assay Kit uses highly purified ADHP toquantify HRP activity in the secondary antibody or streptavidin-HRPconjugates.


71110-M EnzoLyte™ ADHP Peroxidase ELISA 500Assay Kit *Fluorimetric* assays

☛ Other Chromogenic and Fluorogenic Sustratesfor Detecting Peroxidases


85501 ABTS 1 mL[2,2'’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt],1M solution in DMSOPeroxidase substrate suitable for usein ELISA procedures; produce asoluble green product;λ

abs:405 nm; λ

em: N/A

85502 AEC 5 mL[3-Amino-9-Ethylcarbazole],1M solution in DMSOproduce an insoluble red productupon peroxidase/H

2O

2 oxidation

λabs

: N/D; λem

: N/D

85503 4-CN 5 mL[4-Chloro-1-naphthol],1M solution in DMSOproduce an insoluble red productupon peroxidase/H

2O

2 oxidation

λabs

: N/D; λem

: N/D

85504 DAB 5 mL[3,3'’-Diaminobenzidinetetrahydrochloride], 1M aqueoussolution produce an insolublebrown product upon HRP/H

2O

2 oxidation

λabs

: N/D; λem

: N/D

85505 HPPA 1 g[3-(4-Hydroxyphenyl)propionicacid]*UltraPure Grade*Fluorogenic peroxidase substrateλ

abs: 330 nm; λ

em: 410 nm

82100 Luminol *UltraPure Grade* 1 gLuminogenic peroxidase substrate;also used for detecting superoxideλ

abs: 355 nm; λ

em: 411 nm

87350 MCLA 25 mg[2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride]Mainly used for luminogenicdetection of superoxideλ

abs:430 nm; λ

em: 546 nm

85727 NBD methylhydrazine 5 mg[N-Methyl-4-hydrazino-7-nitrobenzofurazan]Flurogenic peroxidase substrateλ

abs:493 nm; λ

em: 552 nm

85506 OPD 5 mL[o-Phenylenediamine dihydrochloride],1M solution in DMSOPeroxidase substrate suitable for use inELISA procedures; produce a solubleyellow-orange productλ

abs: N/D; λ

em: N/D

85507 TMB 1 mL[3,3'’,5,5'’-Tetramethylbenzidinedihydrochloride], 1M solution in DMSOSoluble peroxidase substrate; yields a bluesoluble product with maximum absorptionof 370 and 652 nm that changes toyellow upon addition of strong acidsuch as sulfuric acidλ

abs:492 nm; λ

em: N/D


☛ Enzyme Substrates for Detecting CytochromeP-450 and other Oxidases


85726 Benzyloxyresorufin 10 mg[Resorufin benzyl ether]Fluorogenic cytochrome P-450 substrateλ

abs: 571 nm; λ

em: 585 nm

85703 5-(and-6)-Carboxy-2'’,7'’- 25 mgdichlorodihydrofluorescein diacetateCell-impermeable substrate of oxidases(including peroxidase)λ

abs: 495 nm; λ

em: 529 nm

85702 5-Carboxy-2'’,7'’- 5 mgdichlorodihydrofluoresceindiacetate, di(acetoxymethyl ester)Cell-permeable substrate of oxidases(including peroxidase).λ

abs: 495 nm; λ

em: 529 nm

85708 6-Carboxy-2'’,7'’- 5 mgdichlorodihydrofluorescin diacetate,di(acetoxymethyl ester)Cell-permeable substrate of oxidases(including peroxidase).λ

abs: 495 nm; λ

em: 529 nm

85705 3-Cyano-7-ethoxycoumarin 10 mgFluorogenic cytochrome P-450 substrateλ

abs: 408 nm; λ

em: 450 nm

85706 2'’,7'’-Dichlorodihydrofluorescein 100 mgdiacetate [2'’,7'’-Dichlorofluorescindiacetate]Cell-permeable substrate of oxidases(including peroxidase)λ

abs: 495 nm; λ

em: 529 nm

85700 Dihydrocalcein, AM 1 mgGeneric substrate of oxidases(including peroxidase)λ

abs: 494 nm; λ

em: 517 nm

85701 Dihydrocalcein, AM *Custom 1 mgPackaging*Generic substrate of oxidases(including peroxidase) λ

abs: 494 nm;

λem

: 517 nm

85717 Dihydroethidium [Hydroethidine] 10 mgGeneric substrate of oxidases(including peroxidase); The oxidizedproduct tends to accumulate in nuclei.λ

abs: 518 nm; λ

em: 605 nm

85718 Dihydroethidium 1 mL[Hydroethidine], 5 mM soln. in DMSOGeneric substrate of oxidases (includingperoxidase); The oxidized product tendsto accumulate in nuclei.λ

abs: 518 nm; λ

em: 605 nm

85710 Dihydrofluorescein diacetate [H2FDA] 25 mgGeneric substrate of oxidases (includingperoxidase)λ

abs: 492 nm; λ

em: 517 nm

85711 Dihydrorhodamine 123 10 mgGeneric substrate of oxidases(including peroxidase) in mitochondriaλ

abs: 507 nm; λ

em: 529 nm


85720 Dihydrorhodamine 123 10 mgGeneric redox indicator of oxidases(including peroxidase); The oxidized producttends to accumulate in mitochondria.λ

abs: 507 nm; λ

em: 529 nm

85719 Dihydrorhodamine 123, 5 mM solution 1 mL in DMSOGeneric redox indicator of oxidases(including peroxidase);The oxidized product tends to accumulatein mitochondria.λ

abs: 507 nm; λ

em: 529 nm

85712 Dihydrorhodamine 6G 25 mgGeneric substrate of oxidases(including peroxidase) in mitochondriaλ

abs: 528 nm; λ

em: 551 nm

85715 Ethoxyresorufin 5 mg[Resorufin ethyl ether]Fluorogenic cytochrome P-450 substratethat generates red fluorescent productupon enzyme cleavageλ

abs: 571 nm; λ

em: 585 nm

85716 7-Ethoxy-4-trifluoromethylcoumarin 25 mgFluorogenic cytochrome P-450 substratethat generates blue/green fluorescentproduct upon enzyme cleavageλ

abs: 385 nm; λ

em: 502 nm

85707 Methoxyresorufin 5 mg[Resorufin methyl ether]Fluorogenic cytochrome P-450 substratethat generates red fluorescent productupon enzyme cleavagλ

abs: 571 nm; λ

em: 585 nm

85725 MPTS 100 mg[8-Methoxypyrene-1,3,6-trisulfonic acid,trisodium salt] Cell-impermeablecytochrome P-450 substrate that generatesyellow fluorescent productupon enzyme cleavageλ

abs: 454 nm; λ

em: 511 nm

85740 Resazurin, sodium salt *UltraPure Grade* 100 mgWidely used for measuring cell cytotoxicity,proliferation and mitochondrial metabolicactivity in isolated neural tissue; also usedfor measuring dehydrogenase activityλ

abs: 571 nm; λ

em: 585 nm


82250 D-Luciferin, free acid *UltraPure Grade* 25 mgλ

abs: 328 nm; λ

em: 532 nm

82252 D-Luciferin, potassium salt *UltraPure Grade* 25 mgλ

abs: 328 nm; λ

em: 532 nm

82251 D-Luciferin, sodium salt 25 mg

82251 D-Luciferin, free acid *UltraPure Grade* 25 mgλ

abs: 328 nm; λ

em: 532 nm

☛ Luciferin and Luciferases

Chromogenic and Flurogenic Substrates for Detecting

Cytochrome P-450 and other OxidasesCYTOCHROME P450 & OXIDASES



βββββ- Amyloid Peptides and Related Assay ReagentsAMYLOIDS

Assay kits and reagents

☛ DHL™ fluorescent βββββ-amyloid (1-28) sampler kit

Fluorophore labeled beta-amyloid peptides have been used in investigat-ing beta-amyloid’s aggregation, generation and clearance, microglialactivation and phagocytosis. DHL™ fluorescent b-amyloid (1-28) samplerkit provides eight fluorescent beta-amyloid (1—28) peptides and oneunlabeled peptide as control. The sampler kit provides ample choices forresearchers interested in beta-amyloid related experiments.


71020 DHL™ fluorescent β-amyloid (1-28) 1 kitsampler kit

☛ βββββ-Amyloid (1-28) Tracers

☛ βββββ-Amyloid (1-28), N-Terminal Labeled

(Tag-DAEFRHDSGYEVHHQKLVFFAEDVGSNK)


24231 β-Amyloid (1-28), N-Terminal Unlabeled 0.5 mgAlso available in Biotin andfluor labeled form

☛ DHL™ fluorescent βββββ-amyloid (1-40) sampler kit

Fluorophore labeled beta-amyloid peptides have been used in investigat-ing beta-amyloid’s aggregation, generation and clearance, microglialactivation and phagocytosis. DHL™ fluorescent b-amyloid (1-40) samplerkit provides eight fluorescent beta-amyloid (1—40) peptides and twounlabeled peptide as control. The sampler kit provides ample choices forresearchers interested in beta-amyloid related experiments.

☛ βββββ -Amyloid (1-40) Tracers

βββββ -Amyloid (1-40), N-Terminal Labeled (Tag-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV)




71021 DHL™ fluorescent β-amyloid (1-40) 1 kitsampler kit

☛ DHL™ Fluorescent beta-Amyloid (1-42) Sampler Kit

Fluorophore labeled beta-amyloid peptides have been used in investigat-ing beta-amyloid’s aggregation, generation and clearance, microglialactivation and phagocytosis. DHL™ fluorescent b-amyloid (1-42) samplerkit provides seven fluorescent beta-amyloid (1—42) peptides and twounlabeled peptide as control. The sampler kit provides ample choices forresearchers interested in beta-amyloid related experiments.


71022 DHL™ Fluorescent beta-Amyloid (1-42) 1 kitSampler Kit

☛ βββββ -Amyloid (1-42) Tracers☛ βββββ -Amyloid (1-42), N-Terminal Labeled DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA



☛☛☛☛☛ Anti- β β β β β -Amyloid antibodies


28182 Rabbit Anti-β-Amyloid (anti aa 672-713), 100 µgSp:Human; used in immunoprecipitationimmunofluorescenceAlso available in Biotin andfluor labeled form

29547 Anti-β-Amyloid (NT), monoclonal 0.1 mgSpecies: Human; Host: RabbitAlso available in Biotin andfluor labeled form

☛ Vital Stains for Amyloids


88300 BSB [(trans,trans)-1-Bromo-2,5-bis-(3- 5 mghydroxycarbonyl-4-hydroxy)styrylbenzene]Congo Red derivative, binds to amyloidinclusions in situ and amyloid in brains of liveanimals. Used for monitoring amyloid fibrilsassembled from the Ab peptide, α-synuclein and tau.

88301 BTA-1 [2-(4'’-(methylamino)phenyl)benzothiazole] 10 mguncharged derivative of thioflavin-T that hashigh a affinity for Ab fibrils and shows verygood brain entry and clearance.

88302 BTA-2 [2-(4'’-(dimethylamino)phenyl) 100 mg-6-methyl-benzothiazole]Uncharges derivativeof Thioflavin-T, stains both plaques andneurofibrillary tangles in post mortem Alzheimer’sdisease brain with 6-fold higher affinity.

88303 Chrysamine G 10 mgis a carboxylic acid analogue of Congo red,binds to senile plaques , also used to staincerebrovascular amyloid in tissue sections

83016 Congo Red *UltraPure Grade* 1 gFor early diagnosis of amyloid deposition.Monitored by a yellow-green birefringenceunder crossed polarization. Also, whenobserved under UV light, it emitsfluorescence in amyloid sections.

88305 Half Chrysamine G 10 mgHas lower affinity for Ab than CG.

88306 Thioflavin T *UltraPure Grade* 1 gClassic amyloid stain for senile plaquescontaining bA4 peptide in Alzheimer’s diseasebrain. Binds to sheets, not to monomers/dimmers,when excited at 450 nm, it emits at 482 nm.


� EnzoLyte™ AMC Caspase Substrate Sampler Kit*Fluorimetric*

EnzoLyte™ Caspase Substrate Sample Kit contains a series of AMC-basedpeptide substrates as fluorogenic indicators for assaying caspase pro-tease activities. It provides the best solution for identifying a suitablesubstrate for designing AMC-based caspase assays. All the components inthe kit are also available in bulk package.


71121 EnzoLyte™ AMC Caspase 8x100Substrate Sampler Kit *Fluorimetric* assays

Contents:10 AMC caspase substrates (fluorogenic indicator, Ex/Em=354/422nmupon cleavage), Assay buffer, AMC (fluorescence reference standardfor calibration)

� AnaRed™ based Caspase Assay kits:

AnaRed-based substrates, which yield near IR fluorescence upon pro-teolytic cleavage, are particularly useful for cell-based assays. The kitcan be used to continuously measure the activities of caspase-3 in cellculture directly, cell extracts, and purified enzyme using a fluorescencemicroplate reader or fluorometer.

� EnzoLyte™AnaRed™ Caspase 3 Assay Kit*Fluorimetric*

EnzoLyte™ AnaRed Caspase-3 Assay Kit contains Ac-DEVD-AnaRed™ as afluorogenic indicator for assaying caspase-3 activities and screeningcaspase-3 inhibitors.


71122 EnzoLyte™ AnaRed™ Caspase 3 500Assay Kit *Fluorimetric* assays

Contents:Ac-DEVD-AnaRed substrate (fluorogenic indicator, Ex/Em=595/635nmupon cleavage), AnaRed™ dye (fluorescence reference standard forcalibration), Assay buffer, Ac-DEVD-CHO (inhibitor), Cell lysis buffer

� EnzoLyte™ AnaRed™ Caspase 7 Assay Kit*Fluorimetric*

The EnzoLyte™ AnaRed™ Caspase-7 Assay Kit contains Ac-DEVD-AnaRed™as fluorogenic indicator for assaying Caspase-7 activities and screeningcaspase-7 inhibitors.


71123 EnzoLyte™ AnaRed™ Caspase 5007 Assay Kit *Fluorimetric* assays

Contents:Ac-DEVD-AnaRed substrate (fluorogenic indicator, Ex/Em=595/635nmupon cleavage), AnaRed™ dye (fluorescence reference standard forcalibration), Assay buffer, Ac-DEVD-CHO (inhibitor), Cell lysis buffer,

A detailed protocol

� Caspase substrates, with AnaRed™


60316-1 D-AnaRed™ 1 mgCaspase-2 substrate

60318-1 Z-DEVD-AnaRed™ 1 mgCaspase-3 substrate and caspase-8substrate

60314-1 Ac-IETD-AnaRed™ 1 mgCaspase-8 substrate

� Rh110-based Caspase Assay kits:

Rh110-based substrates, which yield green fluorescence upon proteolyticcleavage. The kit can be used to continuously measure the activities ofcaspase-3 in cell extracts and purified enzyme preparations using afluorescence microplate reader or fluorometer. The longer wavelengthspectrum and higher extinction coefficient of the green-fluorescent Rh110product provide greater sensitivity and less interference from cell com-ponents.

� EnzoLyte™ Rh110 Caspase 3 Assay Kit *MostSensitive*

EnzoLyte™ Rh110 Caspase-3 Assay Kit contains (Ac-DEVD)2-Rh110 as afluorogenic indicator for assaying caspase-3 activities.


71141 EnzoLyte™ Rh110 Caspase 3 Assay Kit 500*Most Sensitive* assays

Contents:(Z-DEVD)2-Rh110 substrate (fluorogenic indicator, Ex/Em=496/520nmupon cleavage), Cell lysis buffer, Assay buffer, Ac-DEVD-CHO (inhibi-tor), Rh110 (fluorescence reference standard for calibration)

� EnzoLyte™ Rh110 Caspase 7 Assay Kit *MostSensitive*

The kit can be used to continuously measure the activities of caspase-7 incell extracts and purified enzyme preparations using a fluorescencemicroplate reader or fluorometer.


71142 EnzoLyte™ Rh110 Caspase 7 Assay Kit 500*Most Sensitive* assays

Contents:(Z-DEVD)2-Rh110 substrate (Ex/Em=496/520 nm upon cleavage), Celllysis buffer, Assay buffer, Ac-DEVD-CHO (inhibitor), Rh110 (fluores-cence reference standard for calibration), A detailed protocol

� EnzoLyte™ Rh110 Caspase 3 Screening Kit *MostSensitive*

This EnzoLyte™ Rh110 Caspase 3 Screening Kit contains (Z-DEVD)2-Rh110as a fluorogenic indicator for screening caspase-3 inhibitors and induc-ers. The kit is used to continuously measure the activities of caspase-3using a fluorescence microplate reader.


71143 EnzoLyte™ Rh110 Caspase 3 Screening Kit 500*Most Sensitive* assays

Contents:(Z-DEVD)2-Rh110 substrate (Ex/Em=496/520 nm upon cleavage), Assaybuffer, Ac-DEVD-CHO (inhibitor), Rh110 (fluorescence referencestandard for calibration), A detailed protocol

� Caspase Substrates, Chromogenic (pNA)pNA (4-nitroaniline)-derived caspase substrates are widely used for thecolorimetric detection of various caspase activities. Cleavage of pNApeptides by caspases generates pNA that is monitored colorimetricallyat ~405 nm. pNA has maximum absorption around 408 nm.


25250-5 Ac-YETD-pNA 5 mgCaspase-1 (ICE) substrate

25259-5 Ac-YVAD-pNA 5 mgCaspase-1 substrate with K

m = 23 mM

and kcat

= 1.5 s-1

25251-5 Ac-VDQQD-pNA 5 mgCaspase-2 substrate

Substrates, Assay kits and Reagents for CaspasesAPOPTOSIS




25252-5 Ac-VDVAD-pNA 5 mgCaspase-2 and k

cat = 4.5 s-1;

Caspase-3 substrate

25253-5 Ac-VQVD-pNA 5 mgCaspase-3 substrate

25282-5 Z-DEVD-pNA 5 mgCaspase-3 substrate

25260-5 Ac-DMQD-pNA 5 mgCaspase-3 substrate

25265-5 Ac-DQMD-pNA 5 mgCaspase-3 (apopain) substrate

25249-5 Ac-LEVD-pNA 5 mgCaspase-4 substrate

25258-5 Ac-IETD-pNA 5 mgCaspase-8 substrate with K

m = 66 mM;

Substrate for granzyme B

25278-5 Ac-LEHD-pNA 5 mgCaspase-9 substrate

25254-5 Ac-VEID-pNA 5 mgCaspase-1, Caspase-3,Caspase-6, Caspase-7

25263-5 Ac-DEVD-pNA 5 mgCaspase-1, Caspase-3, Caspase-4,Caspase-6, Caspase-7, Caspase-8

� Caspase substrates, with Rh110 peptides

Rh110 (rhodamine 110)-derived caspase substrates are probably themost sensitive indicators widely used for the fluorimetric detection ofvarious caspase activities. Cleavage of Rh110 peptides by caspasesgenerates strongly fluorescent Rh110 that is monitored fluorimetricallyat 510-530 nm with excitation of 488 nm, the most common excitationlight source used in fluorescence instruments.

� Caspase Substrates, Fluorogenic (AFC)

AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates arewidely used for the fluorimetric detection of various caspase activities.Cleavage of AFC peptides by caspases generate strongly fluorescent AFCthat is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.


25271-5 Ac-YVAD-AFC 5 mgCaspase-1 substrate

25285-5 Ac-WEHD-AFC 5 mgCaspase-1 substrate

25264-5 Ac-VDVAD-AFC 5 mgCaspase-2 substrate

60325-5 Ac-DMQD-AFC 5 mgCaspase-3 substrate

25273-5 Ac-DEVD-AFC 5 mgCaspase-3 andCaspase-7 substrate

21685-5 Z-DEVD-AFC 5 mgCaspase-3 and caspase-7 substrates

28267 Ac-LEVD-AFC 5 mgCaspase-4 substrate

25272-5 Ac-VEID-AFC 5 mgCaspase-6 substrate

25270-5 Ac-IETD-AFC 5 mgCaspase-8 substrate

60324-5 Z-IETD-AFC 5 mgCaspase-8 substrate

25276-5 Ac-LEHD-AFC 5 mgCaspase-9 substrate


28267-5 Ac-YEVD-AMC 5 mgCaspase-1 (ICE) substrate

25275-5 Ac-WEHD-AMC 5 mgCaspase-1 substrate with Km/K

cat = 3.3×106 M-1s-1

25267-5 Ac-YEVD-AMC 5 mgCaspase-1 (ICE) substrate

25261-5 Ac-YVAD-AMC 5 mgCaspase-1 and Caspase-4 substrate

25255-5 Ac-VDVAD-AMC 5 mgCaspase-2 substrate

25266-5 Ac-DMQD-AMC 5 mgCaspase-3 substrate

25262-5 Ac-DEVD-AMC 5 mgCaspase-3 and Caspase-7 substrate

60303-5 Z-DEVD-AMC 5 mgCaspase-3 and caspase-7 substrates

28265 Ac-LEVD-AMC 5 mgCaspase-4 substrate

25256-5 Ac-VEID-AMC 5 mgCaspase-6 substrate

25256-5 Ac-IETD-AMC 5 mgCaspase-8 substrate

25257-5 Ac-IETD-AMC 5 mgCaspase-8 substrate

25286-5 Ac-LEHD-AMC 5 mgCaspase-9 substrate

� Caspase Substrates, Fluorogenic (AMC)

Substrates for CaspasesAPOPTOSIS


� Caspase Substrates, Chromogenic (pNA)


60310-1 (Ac-YVAD)2-Rh110 1 mg

Caspase-1 substrate

60309-1 (Ac-WEHD) 2-Rh110 1 mg

Caspase-1 substrate

60313-1 D2-Rh110 1 mg

Caspase-2 substrate

60304-5 (Z-DEVD) 2-Rh110 5 mg

Caspase-3 substrate and caspase-7substrate

60305-1 (Ac-DMQD) 2-Rh110 1 mg

Caspase-3 substrate

60307-1 (Ac-LEVD) 2-Rh110 1 mg

Caspase-4 substrate

60308-1 (Ac-VEID) 2-Rh110 1 mg

Caspase-6 substrate

60312-1 (Z-IETD) 2-Rh110 5 mg

Caspase-8 substrate

60306-1 (Ac-LEHD) 2-Rh110 1 mg

Caspase-9 substrate

� Caspase Antibodies

Please inquire


GTP Family- RasSIGNAL TRANSDUCTION

GTP-Ras

� Small GTPases (Ras Superfamily) - Ras Family

GTPases of the Ras superfamily represent a group of more than fiftyproteins that function as molecular switches controlling a variety ofsignaling and transport pathways essential for numerous cellular functions.They were grouped, according to sequence homologies, into five principalbranches: Ras family, Rab family, Ran family, Rho/Rac/Cdc42 family andSar1/Arf family. Mutations in Ras proteins that block them in aconstitutively active state are found in 30 to 60% of human malignancies.Ras-proteins have a molecular mass of 21 kDa. Suitable as a substrate forfarnesyltransferase.

H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B.

H-Ras∆C comprises the first 166 residues of Ras that are sufficient forbinding guanine nucleotides and hydrolysis of GTP, but lacks the C-terminalCaaX motif necessary for membrane localization of Ras.

H-Ras∆C (aa 1 - 171) lacks the C-terminus with the CaaX recognitionsequence necessary for anchoring Ras into the plasma membrane. Themutation S17N results in a 40-fold increase in the affinity for GTP withoutaffecting its affinity for GDP.

The mutation Q61L results in a decreased GTPase activity as well asincreased GDP/GTP exchange. This mutant constitutively activates theRas-signaling pathway.

The mutation G12V leads to elimination of the intrinsic GTPase activity.H-Ras (G12V) is effective in activation of PI3K and PKB, whereas N-Rasand K-Ras are more potent towards MAP kinase.

K-Ras, one of three classic human Ras genes, is spliced to two differentisoforms, K-Ras4A and K-Ras4B. K-Ras4B is the predominant product ofthe alternative splicing pathway and the Ras protein encoded by thecommonly mutated Ras gene in human cancer.

Stimulation of Ba/F3-Fms cells with either IL-3 or CSF-1 resulted in efficientactivation of both K-Ras 4B and M-Ras. Stimulation of fibroblasts withEGF also led to more efficient activation of K-Ras 4B than of H-Ras or N-Ras, and the activation of M-Ras was very strong.

An S27N mutant of M-Ras, like the analogous H-Ras S17N mutant, was adominant inhibitor of activation of the c-fos promoter by constitutivelyactive Src Y527F, suggesting that M-Ras and p21 Ras shared guaninenucleotide exchange factors and are likely to be activated in parallel. M-Ras (Q71L) is a constitutive active mutant of M-Ras.

N-Ras ∆C comprises the first 167 residues of Ras that are sufficient forbinding guanine nucleotides and hydrolysis of GTP, but lacks the C-terminalCaaX motif necessary for membrane localization of Ras.

TC21 is a Ras-like GTPase with high oncogenic potential that is foundmutated in some human tumors and overexpressed in breast cancer celllines. TC21 binds physically to c-Raf-1 in a GTP-dependent manner.

TC21 (Q72L) is a constitutive active mutant of TC21.

Rap1A, in contrast to Ras, is found largely in mid-Golgi, endocyticvesicles, and lysosomal vesicles, indicating different cellular functions ofthe proteins. In developmental systems, the functions of Rap proteinsseem to be related primarily to morphological and differentiative events,rather than to those that govern proliferation and cell-fate specification,phenomena that often require signaling via conventional Ras proteins.

The G12V mutant is a constitutively active mutant of Rap1A.

Rap1B is known to antagonize the mitotic and transforming activity ofRas. Rap1B can be activated by cAMP known to either stimulate orinhibit cell proliferation. The deletion of 17 C-terminal amino acids inRap1B ∆C leads to the loss of the phosphorylation site Ser179 and thegeranyl-geranylation site.


PR-107 H-Ras 50 µg(Harvey rat sarcoma viral oncogene homolog)Human

PR-364 H-RasGST 50 µg(Harvey rat sarcoma viral oncogenehomolog)Human

PR-201 H-Ras ∆C 50 µg(Harvey rat sarcoma viral oncogenehomolog, residues 1-166)Human

PR-202 H-Ras ∆C (S17N) 50 µg(Harvey rat sarcoma viral oncogenehomolog, residues 1-166)Human

PR-203 H-Ras (Q61L) 50 µg(Harvey rat sarcoma viraloncogene homolog)Human

PR-206 H-Ras (G12V) 50 µg(Harvey rat sarcoma viraloncogene homolog)Human

PR-358 H-Ras (G12V)GST 50 µg(Harvey rat sarcoma viral oncogenehomolog)Human

PR-204 K-Ras 4B 50 µg(Kirsten rat sarcoma viraloncogene homolog, isoform 4B)Human

PR-396 M-Ras/R-Ras3His (Q71L) 50 µg(muscle and microspikes Ras,related ras viral oncogene homolog 3)Human

PR-205 N-Ras ∆C 50 µg(Neuroblastoma rat sarcomaviral oncogene homolog, residues 1-167)Human

PR-359 TC21/R-Ras2GST 50 µg(Teratocarcinoma oncogene,related ras viral oncogene homolog 2)Human

PR-395 TC21/R-Ras2His (Q72L) 50 µg(Teratocarcinoma oncogene,related ras viral oncogene homolog 2,constitutive active mutant)Human

PR-124 Rap1AHis 50 µg(Ras-proximate 1A)Human

PR-125 Rap1AHis (G12V) 50 µg(Ras-proximate 1A)Human

PR-301 Rap1B ∆C 50 µg(Ras-proximate 1B, deletion of17 C-terminal residues)Human


� Small GTPases (Ras Superfamily) - Rab Family

Rab proteins are small GTP-binding proteins that form the largest familywithin the Ras superfamily. Rab proteins regulate vesicular traffickingpathways, behaving as membrane-associated molecular switches.

Rab1A is a small GTPase that belongs to the Ras superfamily. Rab proteinsplay an important role in various aspects of membrane traffic, includingcargo selection, vesicle budding, vesicle motility, tethering, docking,and fusion. For example, Rab1A, Rab1B and Rab2 are located inthe ER toGolgi intermediate compartment and cis Golgi cisternae and are requiredfor ER to Golgi and intra Golgi transport.

The small GTPase Rab2 is a resident of pre-Golgi intermediates and isrequired for protein transport from the endoplasmic reticulum to theGolgi complex.

Rab3A, a member of the Rab small G protein family, is involved in theprocess of Ca2+-dependent neurotransmitter release. Rab3A activity isregulated by a GDP/GTP exchange protein (Rab3 GEP), a Rab GDPdissociation inhibitor (Rab GDI), and a GTPaseactivating protein (Rab3GAP). The Rab3A recycling is coupled with synaptic vesicle trafficking.

The monomeric GTPase Rab4 is associated with early endosomes andregulates recycling vesicle formation. Together with Rab5 it act to controlinflux and efflux out of early endosomes.

Rab6A is a small GTPase that belongs to the Ras superfamily. It mediatesintra-Golgi vesicular trafficking and is geranylgeranylated on both C-terminal cysteines.

Rab7 is a 23.5 kDa small GTPase localized in late endosomes. Rab7 regulateslate endocytic membrane traffic between endosomes and lysosomes. Theprotein contains a C-terminal CXC motif for post-translationalgeranylgeranylation.

The small GTPase Rab17 is restricted to epithelial cells and its expressionis induced during cell polarization. In addition to the network of ubiquitousRab GTPases, a set of epithelia-specific Rab proteins, including Rab17,Rab18, Rab20, and Rab25, facilitates endocytic and transcytotic transportto the apical and basolateral plasma membranes.

Rab24 exists predominantly in the GTP state when expressed in culturedcells. The low GTPase activity is related to the presence of serine insteadof glutamine at the position cognate to Ras Gln-61. Rab24 was found inthe endoplasmic reticulum/cis-Golgi region and on late endosomalstructures. The localization of Rab24 may indicate its involvement inautophagyrelated processes.

The monomeric GTPase Rab27 subfamily regulates the exocytosis of thesecell-specific store organelles. Rab27 acts through organelle-specificeffector proteins, such as granuphilin in pancreatic beta cells andmelanophilin in melanocytes. The Rab27 and effector complex theninteracts with proteins that are essential for membrane transport andfusion.

Rab GDP Dissociation Inhibitor (RabGDI) forms complexes with cytosolicprenylated GDP-bound Rab proteins and delivers them to the targetmembrane. It also retreaves GDP-bound Rab proteins from the membranereleases.


PR-113 Rab1AGST-His 50 µg(Ras-associated, small GTP-binding protein)Mouse

PR-114 Rab1BGST-His 50 µg(Ras-associated, small GTP-binding protein)Rat

PR-111 Rab2 50 µg(Ras-associated, small GTP-binding protein)Human

PR-112 Rab2GST-His 50 µg(Ras-associated, small GTP-binding protein)Human

PR-115 Rab3AGST-His 50 µg(Ras-associated, small GTP-binding protein)Mouse

PR-117 Rab4A 50 µg(Ras-associated, small GTP-binding protein)Human

PR-116 Rab4AGST-His 50 µg(Ras-associated, small GTP-binding protein)Human

PR-109 Rab6A 50 µg(Member of Ras oncogene family)Human

PR-108 Rab7 50 µg(Ras-associated, small GTP-binding protein)Dog

PR-362 Rab7GST-His 50 µg(Ras-associated, small GTP-binding protein)Dog

PR-119 Rab17 50 µg(Ras-associated, small GTP-binding protein)Mouse

PR-118 Rab17GST-His 50 µg(Ras-associated, small GTP-binding protein)Mouse

PR-121 Rab24 50 µg(Ras-associated, small GTP-binding protein)Mouse

PR-120 Rab24GST-His 50 µg(Ras-associated, small GTP-binding protein)Mouse

PR-123 Rab27A 50 µg(Ras-associated, small GTP-binding protein)Rat

PR-122 Rab27AGST-His 50 µg(Ras-associated, small GTP-binding protein)Rat

PR-110 RabGDI 50 µg(Rab GDP Dissociation Inhibitor,member of Ras oncogene family)Bovine, Sf9 insect cells

GTP Family- RabSIGNAL TRANSDUCTION

GTP- Rab


� Small GTPases - Ran Family

Ran (Ras-related nuclear protein) is involved in diverse cellular pro-cesses like nucleo-cytosplasmic transport of proteins into the nucleusand RNA into the cytoplasm, mitotic spindle assembly, and post-mitoticnuclear assembly. The Ran-regulated nucleo-cytoplasmic transport throughthe nuclear core complex occurs in conjunction with importins andexportins.

The mutation T24N results in no or little binding to GTP and is known toact as a negative inhibitor of nuclear import.

Ran Q69L is defective in GTP hydrolysis. Useful as a negative control forRan binding and import studies.

The mutation Q69L abolishes GTP hydrolysis. The mutation G19V resultsin a gain-of-function mutant that is not, however, sensitive to the ex-change factor RCC1.

The mutation E70A results in inhibition of the guanine exchange reactionmediated by RCC1, the guanine nucleotide exchange factor (GEF) forRan.


PR-211 Ran 50 µg(Ras-related nuclear protein) Human

PR-212 Ran (T24N) 50 µg(Ras-related nuclear protein) Human

PR-213 Ran (Q69L) 50 µg(Ras-related nuclear protein) Human

PR-214 Ran (G19V/Q69L) 50 µg(Ras-related nuclear protein) Human

PR-215 Ran (E70A) 50 µg(Ras-related nuclear protein) Human

� Small GTPases - Rho/Rac/Cdc42 Family

Rho family GTPases Rac1 and Cdc42 (cell division cycle 42) belong to theRas superfamily of small GTP-binding proteins. The human homolog ofyeast Cdc42 is essential for cell polarity and regulates cytoskeletal rear-rangements in responses to growth factor stimulation.

The C-terminal deletion of 13 amino acids of Cdc42 DC includes thepolybasic domain consisting of six contiguous basic amino acids. Thepolybasic domain of Cdc42 is required for homodimer formation.

The Gly12 to Val mutation of Cdc42 DC leads to a constitutive activeprotein.

Rac1 is involved in regulation of cell growth to cytoskeletal organisationrequired for cell motility and cell adhesion.The C-terminal deletion offive amino acids of Rac1DC (aa 1 - 187) includes arginine 188, which ispart of the polybasic domain consisting of six contiguous basic aminoacids. Deletion or mutation of the polybasic residues decrease the in-trinsic GTPase activity and result in a loss of the self-stimulatory GAPactivity.

RhoA is a small GTPase of the Ras superfamily. Regulates stress fiberformation in response to growth factor stimulation.


PR-302 Cdc42 ∆∆∆∆∆CGST 50 µg(Cell Division Cycle Protein 42,C-terminal deletion of 13 residues) Human

PR-300 Cdc42 ∆∆∆∆∆C G12VGST 50 µg(Cell Division Cycle Protein 42, G12Vmutant, C-terminal deletion of 13 residues)Human

� Heterotrimeric G-Proteins

Heterotrimeric G-Proteins are GTPases composed of α-, β-, and γ-subunits. These GTPases are classically associated with plasma membranereceptors containing seven transmembrane domains (G protein-coupledreceptors [GPCRs]). Receptor activation activates the G-Protein byinducing the exchange of GTP for GDP at a binding site on Gααααα. Gααααα and/orGβγ, then goes on to interact with effector proteins. In mammals, about20 Gααααα subunits (39 to 46 kD), about 5 distinct Gβ (ca. 35 kD) and at least12 Gγ subunits (from 7 to 10 kD) have been identified.

GsαααααL is the long splice variant of the α-subunit of stimulatoryheterotrimeric Gs-proteins. It differs from the short splice variant(GsαααααS) by 15-amino acid insert between the Ras-like domain and the a-helical domain. GsαL activates adenylate cyclase (AC) and possesses alower GDP-affinity than GsαS (cat.# PR-505). These differences in GDP-affinity have important consequences for receptor/G-protein couplingand AC activation.

The mutant GsαααααL-Asn295 is functionally inactive in terms of nucleotidebinding. GTP-binding possesses a highly conserved aspartate residue inthe NKXD motif that is critical for high-affinity interaction with GTP. Inalmost all GTP-binding proteins so far, the D/N-mutation switches base-specifity from guanine to xanthine.

The exchange of Gln227 to Leu227 inhibits the intrinsic GTPase activity,resulting in a constitutively active Gααααα, and increases GDP-affinity of Gααααα.Whereas the exchange of Asp295 to Asn295 leads to inactive mutants ofGα-subunits, an additional Q/L-mutation in the catalytic site (Gln227 →Leu227) rescues protein function and induces xanthine nucleotide-specifity.

In contrast to all other Gααααα-subunits studied so far, the D/N-mutation inGsαS does not influence nucleotide binding activity.

The exchange of Gln212 to Leu212 inhibits the intrinsic GTPase activity,resulting in a constitutively activated Gααααα, and increases GDP-affinity ofGααααα.

Gustducin is a transducin-like heterotrimeric guanine nucleotide-bindingprotein (G-protein) expressed in taste receptor cells (TRCs) of thetongue, stomach and intestinal brush cells. Gustducin was demonstratedto be myristolated and was also palmitoylated in insect cells. Gustducinmediates two responses, i.e. a decrease in cyclic nucleotidemonophosphates via activation of phosphodiesterase 1A by gustducin-αand activation of phospholipase C-β by released Gβγ subunits. Gustducin-α has close structural similarity with the visual G-protein, transducin-α.Gustducin-α reconstituted with the G-protein subunits Gβ1γ2 was preparedfrom Sf9 cells coinfected with gustducin-α and Gβ1γ2-encodingbaculoviruses.


PR-303 Rac1 ∆∆∆∆∆C 50 µg(Ras-related C3 botulinum toxin substrate 1,residues 1-187) Human

PR-106 RhoA 50 µg(Ras-like GTP-binding protein)Human

PR-363 RhoAGST 50 µg(Ras-like GTP-binding protein, GST-tagged)Human


PR-501 GsαααααL 1 ml

(stimulatory heterotrimeric G-protein,long splice variant of the α-subunit)Rat

PR-502 GsαααααL-Asn295 1 ml

(stimulatory heterotrimeric G-protein,long splice variant of the α-subunit)Rat

GTP Family- Ran, Heterotrimeric G-proteinSIGNAL TRANSDUCTION

GTP-Ran, Heterotrimer


� Heterotrimeric G-Proteins


PR-504 GsαααααL

-Leu227 1 ml(stimulatory heterotrimeric G-protein,long splice variant of the α-subunit)Rat

PR-503 GsαααααL-Leu227-Asn295 1 ml

(stimulatory heterotrimeric G-protein,long splice variant of the α-subunit) Rat

PR-505 GsαααααS

1 ml(stimulatory heterotrimeric G-protein,short splice variant of the α-subunit) Rat

PR-507 GsαααααS-Asn280 1 ml

(stimulatory heterotrimeric G-protein,short splice variant of the α-subunit) Rat


-Leu212 1 ml(stimulatory heterotrimeric G-protein,short splice variant of the α-subunit) Rat


-Leu212-Asn280 1 ml(stimulatory heterotrimeric G-protein,short splice variant of the α-subunit) Rat

PR-601 Gustducin + βββββ1γ2

1 ml(Gustducin + G-protein ß

1γ

2-subunits) Rat

� GEFs (Guanine Nucleotide Exchange Factors)

EPAC (exchange protein directly activated by cAMP) is a Rap-specificguanine-nucleotide exchange factor (GEF). EPAC is activated by the bind-ing of cAMP to a cyclic nucleotide monophosphate-binding domain. N-terminal deletion of the DEP domain (domain that occur in Dishevelled,Egl-10, and Pleckstrin) of EPAC∆DEP does not affect regulation of EPAC-activity but affects membrane localization.

RCC1 (Regulator of Chromosome Condensation, a β-propeller chromatin-bound protein) catalyzes guanine nucleotide exchange of the Ras-relatednuclear protein Ran. RCC1 is the guanine nucleotide exchange factor(GEF) for Ran that induces exchange between the bound GDP and cellularGTP. Genetic studies have suggested that RCC1 may be involved in sens-ing the replication state of DNA and controlling the activation of Cdc2/cyclin B protein kinase through Ran.

Ras-binding domain (RBD) of the human RalGDS, comprises amino acids1 - 97 of wild type.

RalGDS (Ral-guanine nucleotide dissociation stimulator) is the guaninenucleotide exchange factor (GEF) for the Ras-related protein Ral. TheGEF-activity of RalGDS is stimulated by binding of Ras.


PR-304 EPAC-1 ∆DEP 50 µg(Exchange Protein directly Activated by cAMP,lacks the Dishevelled, Egl-10 and PleckstrinDomain) Human

PR-306 RCC1 (RanGEF) 50 µg(Regulator of Chromosome Condensation,Ran Guanine Exchange Factor) Human

PR-365 RalGDS-RBDGST 50 µg(Ras Binding Domain of the Ral GuanineNucleotide Dissociation Stimulator) Human

� GAPs (GTPase Activation Proteins)

GTPase activating proteins (GAPs) accelerate the GTP hydrolysis reactionby up to 5 orders of magnitude, thus switching “off“ the activatedGTPase and terminating the signaling activity. The malignanttransformation of cells is often linked to oncogenic mutations of Ras thatescape regulation by GAPs.

The NF1 (Neurofibromin) gene product acts as a GTPase activating factor(GAP) on Ras. Inactivating mutations in NF1 lead to neurofibromatosistype 1. NF1- 333 contains residues 1198-1530 of human NF1 comprisingthe functional GAP-related domain that is able to stimulate GTP-hydrolysison wild type Ras.

RapGAP, the GTPase activating protein of Rap, catalyzes the transitionof Rap from GTP-bound to GDP-bound state by hydrolysis of GTP. Rapproteins are GTPases of the Ras superfamily of G proteins opposing theRas activation pathway. RapGap-341 is a catalytic active fragmentcomprising 341 amino acids (residues 75 - 415) of wild type Rap1GAP.RapGAP-341 (R91A) retained more than 50% activity in comparison toRapGAP-341.

Rna1p is the GTPase-activating factor (GAP) of yeast Gsp1p, an orthologof mammalian Ran. Rna1p is located in the cytoplasm throughout the cellcycle and plays a direct role in protein import into the nucleus in S.

cerevisiae and S. pombe.

� GTPase Cycle Effectors

Raf is a member of the serine/threonine proteine kinase family involvedin regulation of cell growth and differentiation and is the most impor-tant effector of Ras. RafRBD (Ras binding domain, amino acids 50-132)mediates interaction with membrane-anchored Ras necessary for acti-vation of the kinase activity of Raf.

Ras-binding domain (RBD) of the human RalGDS, comprises amino acids1 - 97 of wild type. RalGDS (Ral-guanine nucleotide dissociation stimula-tor) is the guanine nucleotide exchange factor (GEF) for the Ras-relatedprotein Ral. The GEF-activity of RalGDS is stimulated by binding of Ras.

Rhotekin is an effector of the Rho-GTPases that was previously identi-fied in mice.


PR-223 NF1-333 (RasGAP) 50 µg(Neurofibromin, GTPase ActivatingProtein of Ras) Human

PR-222 RapGAP-341 (R91A) 50 µg(GTPase Activating Protein of Rap)Human

PR-221 Rna1p (RanGAP) 50 µg(GTPase Activating Factor of yeastGsp1p, an ortholog of mammalian Ran)S. pombe

GTP Family- GTPases, GPCRsSIGNAL TRANSDUCTION

GTP Heterotrimer, GAP, GTPase


PR-305 RafRBD (c-Raf) 50 µg(Ras Binding Domain of Raf,c-Raf 1 Kinase) Human

PR-366 RafRBDGST (c-Raf) 50 µg(Ras Binding Domain of Raf, c-Raf1 Kinase) Human

PR-365 RalGDS-RBDGST 50 µg(Ras Binding Domain of the Ral GuanineNucleotide Dissociation Stimulator)Human

PR-367 RhotekinRBDGST 50 µg(Rhotekin Ras Binding Domain, fromthe Japanese Human


� GTPase Cycle Effectors

The Wiskott-Aldrich syndrome protein (WASP) is the founding memberof a family of proteins that have emerged as crucial effectors of RhoGTPases and activators of the cytosceletal organizing complex Arp2/3.WASP has been shown to be intimately involved in many pathways thatinfluence the function of the immune system. WASP-121 comprises theamino acids 201-321 of the native WASP sequence. This domain is essen-tial for the binding of Cdc42 to WASP.

EPAC (exchange protein directly activated by cAMP) is a Rap-specificguanine-nucleotide exchange factor (GEF). EPAC is activated by the bind-ing of cAMP to a cyclic nucleotide monophosphate-binding domain. N-terminal deletion of the DEP domain (domain that occur in Dishevelled,Egl-10, and Pleckstrin) of EPAC∆DEP does not affect regulation of EPAC-activity but affects membrane localization.

The amino terminus of Af6 was identified as the Ras-binding site. Addi-tionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved inthe formation of tight junctions, also binds to Af6 close to the aminoterminus thereby competing with Ras binding. These data suggest aparticipation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1.

RIN2, only expressed in neurons, preferentially interacts with the GTPform of Rab5 rather than the GDP form, although it enhances the guanineexchange reaction on Rab5. Additionally RIN2 interacts with several Rasproteins, it binds the GTP-loaded forms of H-Ras, TC21 and M-Ras.

� GTPase Modifying Enzymes

GGTase-I (Geranylgeranyltransferase-I) catalyzes the transfer of thefarnesyl and geranylgeranyl groups from farnesyl and geranylgeranyl-diphosphate to proteins containing a C-terminal CaaX motif.Farnesyltransferase (FT) and GGTase-I are closely related, sharing acommon a subunit and 30% identity in their β subunits.

GGTase-II (Geranylgeranyltransferase-II) catalyzes the transfer ofgeranylgeranyl moiety onto two C-terminal cysteines of Rab proteins.Composed of an α and β heterodimer (50 and 38 kDa, respectively) andrequires Rab escort protein for its catalytic activity. GGTase-II wasshown to exhibit higher affinity towards geranylgeranyl pyrophosphate(Kd = 8 nM) than farnesyl pyrophosphate (Kd = 60 nM).

FTase catalyzes the transfer of the farnesyl group from farnesyl diphos-phate to proteins containing a C-terminal CaaX motif, where ’C’ is aconserved cysteine that is the site of farnesyl modification, ’a’ is usuallyan aliphatic amino acid, and ’X’ is methionine, serine, glutamine, oralanine.

REP-1 (Rab escort protein-1) binds to a wide range of RabGGTase withnanomolar affinities and supports the prenylation of RabGTPase medi-ated by RabGGTase.

� GPCRs (G-Protein Coupled Receptors) -Adrenergic Receptors

The human β1-adrenoreceptor (β1AR) is activated by the catecholaminesepinephrine and norepinephrine and couples to the G-protein Gs tomediate adenylate cyclase activation. The β1AR exists as several poly-morphic forms of which the Gly389 and Arg389 variants are among thebest known. There is a controversy whether or not there are functionaldifferences between the two β1AR polymorphisms. β1ARs are mainlyfound in the heart, kidney, and fat tissue. These receptors are involvedin physiological processes such heart contraction, renin release and li-polysis..

β1-Adrenergic receptor-Gly389-GsαL is a fusion protein in which the GsαLN-terminus is linked to the β

1-adrenoceptor (β1AR) C-terminus via a

hexahistidine (His6)-tag. GsαL is the long splice variant of the α-subunitof the heterotrimeric G-protein Gs. The β1AR-Gly389-GsαL fusion pro-tein ensures a defined 1:1 stoichiometry of the receptor and the GsαLsubunit as well as high coupling efficiency. In contrast to β1AR-Arg389-GsαS (cat.# PR-524), α1AR-Gly389-GsαL exhibits hallmarks of high con-stitutive activity, i.e. high efficacy and potency of partial agonists atactivating [35S]GTPαS binding and high efficiency of agonist-free β1AR-Gly389-GsαL at activating AC.

β1-Adrenergic receptor-Arg389-GsαL is a fusion protein in which theGs?L N-terminus is linked to the β1-adrenoreceptor (β1AR) C-terminusvia a (His6)-tag. The β1AR exists as several polymorphic forms of whichthe Gly389 and Arg389 variants are among the best known.

β2-Adrenergic receptorCAM-GsαL is a fusion protein in which the GsαLN-terminus is linked to the β

2AR

CAM C-terminus via (His6)-tag. β

2AR

CAM is

a constitutively active mutant of the β2AR. It differs from the wild-typereceptor by four discrete amino acid substitutions in the third intracel-lular loop of the receptor (L266 → S266, K267 → R267, H269 → K269, andL272 → A272).

β2-Adrenergic receptor-GsαL is a fusion protein in which the GsαLN-terminus is linked to the β2-adrenoceptor (β

2AR) C-terminus via a

(His6)-tag.

� GTPase Modifying Enzymes

REP-2 (Rab escort protein-2) binds to a wide range of RabGGTase withnanomolar affinities and supports the prenylation of RabGTPase medi-ated by RabGGTase, with an exception of Rab27.


PR-101 GGTase-I 50 µg(Geranyl-Geranyl-Transferase) Rat

PR-360 GGTase-IGST 50 µg(Geranyl-Geranyl-Transferase) Rat

PR-103 GGTase-II (Rab GGTase) 50 µg(Geranyl-Geranyl-Transferase, α- andβ-subunit) Rat

PR-102 FTase 50 µg(Protein farnesyltransferase, α- andβ-subunit) Rat

PR-361 FTaseGST 50 µg(Protein farnesyltransferase, α- andβ-subunit) Rat

PR-105 REP-1His 50 µg(Rab Escort Protein) Rat

PR-104 REP-2His 50 µg(Rab Escort Protein) Human

GTP Family- Ran, Heterotrimeric G-proteinSIGNAL TRANSDUCTION

GTPase, GPCRs


PR-369 WASP-121GST 50 µg(Wiskott-Aldrich Syndrome Protein) Human

PR-304 EPAC-1 ∆∆∆∆∆DEP 50 µg(Exchange Protein directly Activatedby cAMP, lacks the Dishevelled, Egl-10and Pleckstrin Domain) Human

PR-398 AF6-RBDGST (residues 1-141) 50 µg(GTP-H-Ras binding cytoplasmic protein,Afadin - Ras-binding domain) Human

PR-397 RIN2-RAGST (residues 785-879) 50 µg(Ras and Rab interactor 2 - Ras-association domain) Human

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� GPCRs (G-Protein Coupled Receptors) -Adrenergic Receptors

β2-Adrenergic receptor-GsαL-Leu227 is a fusion protein in which the GsαL-Leu 227 N-terminus is l inked to the β2-adrenoceptor (β

2AR)

C-terminus via a (His6)-tag.The exchange of Gln227 to Leu227 (Q/L mutation)in the catalytic site abolishes the intrinsic GTPase activity and increasesthe GDP-affinity of Gsα, resulting in a constitutively activeG-protein.

β2-Adrenergic receptor-GsαL-Leu227-Asn295 is a fusion protein in which

the GsαL-Leu227-Asn295 N-terminus is linked to the β2-adrenoceptor (β

2AR)

C-terminus via (His6)-tag. The exchange of Asp295 to Asn295 leads to aninactive Gsα-mutant. However, an additional Q/L-mutation in the cata-lytic site (Gln227 → Leu227) that abolishes GTPase activity and increasesGDP-affinity rescues protein function and induces specificity for XTPand XppNHp relative to GTP and GppNHp, respectively.

β2-Adrenergic receptor-GsαL is a fusion protein in which the GsαL

N-terminus is linked to the β2AR C-terminus via a (His6)-tag. Gβ

1γ

2 are

subunits of the heterotrimeric G-protein.

GTP Family- GPCRsSIGNAL TRANSDUCTION

GPCRs


PR-521 βββββ1-AR-Gly389 1 ml

(β1-Adrenergic Receptor) Human

PR-522 βββββ1-AR-Gly389-G

sαααααL1 ml

(β1-Adrenergic Receptor G

sαL fusion protein) Human

PR-525 βββββ1-AR-Arg389-G

sαααααL1 ml



PR-524 βββββ1-AR-Arg389-G

sαS1 ml


sαS fusion protein) Human

PR-523 βββββ1-AR-Gly389-G

sαααααS1 ml


sαS fusion protein) Human

PR-531 βββββ2-AR

CAM-G

sαL1 ml

(Constitutively Active Mutant of ß2-Adrenergic

Receptor GsαL

fusion protein) Human

PR-532 βββββ2-AR-G

sαααααL1 ml




saL-Leu227 1 ml




saL-Leu227-Asn295 1 ml




saL + ß

1γγγγγ

21 ml


sαL fusion protein + ß

1γ

2-

subunits) Human

PR-536 βββββ2-AR-TS-G

sαααααL1 ml


sαL fusion protein

with a thrombin cleavage site) Human

PR-537 βββββ2-AR + G

sαααααL1 ml

(β2-Adrenergic Receptor + G

sαL) Human


qα 1 ml


qα fusion protein) Human


iααααα21 ml


iα2 fusion protein) Human


iααααα2 + βββββ

1α

21 ml


iα2 fusion protein +

β!α

2-subunits) Human

� GPCRs (G-Protein Coupled Receptors) -Histamine Receptors

The histamine H1-receptor (H1R) belongs to the superfamily of seventransmembrane-domain (7TM), G-protein-coupled receptors (GPCRs).The endogenous agonist for the H1R is histamine, which is both neu-rotransmitter and autacoid. The H1R couples to Gq-proteins to activatephospholipase C. Numerous agonists and antagonists are known. H1Ragonists are divided into three classes, i.e. small agonists derived fromhistamine, histamine derivatives with bulkier aromatic substituents atposition 2 of the imidazole ring and histaprofidens.

The regulator of G-protein signaling 4 (RGS4) efficiently enhances steady-state GTP hydrolysis stimulated by H1R, thus providing a very sensitivemodel system for the pharmacological analysis of the H1R directly at theG-protein level.

The guinea pig H2R (gpH2R) was prepared from Sf9 cells infected withgpH2R-encoding baculovirus. In this system, gpH2R couples to the Gsα-like G-proteins of the insect cells to mediate AC activation.

hH2R-Gqα is a fusion protein in which the Gqα N-terminus is linked to theC-terminus of the human H2-receptor (H2R) via a (His6)-tag.

hH2R-GsαL is a fusion protein in wich the GsαL N-terminus is linked to theC-terminus of the human H2-receptor (hH2R) via a (His6)-tag.

hH2R-GsαS is a fusion protein in wich the GsαS N-terminus is linked to theC-terminus of human H2-receptor (hH2R) via a (His6)-tag.

hH2R reconstituted with GsαS was prepared from Sf9 cells coinfectedwith hH2R- and GsαS-encoding baculoviruses.



iααααα31 ml


iα3 fusion protein)

Human


iααααα3 + ß

1γγγγγ

21 ml


iα3 fusion protein

+ β1γ

2-subunits) Human


qααααα + βββββ1γγγγγ

21 ml


qα fusionprotein + β

1γ

2-subunits) Human


iααααα31 ml


iα3 fusion protein)

Human


sαααααS1 ml


sαS fusion protein)

Human


sαααααS-Asn280 1 ml



Human, Recombinant, Sf9 insect cells


sαααααS-Leu212-Asn280 1 ml



Human


sαααααS-Leu212 1 ml



Human

PR-548 βββββ2-AR + G

sαααααS1 ml

(β2-Adrenergic Receptor + G

sαS) Human


16ααααα 1 ml(β

2-Adrenergic Receptor G

16α fusion protein)Human


16ααααα + β1γγγγγ

21 ml


16ααααα fusionprotein + β

1γ

2-subunits) Human

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GTP Family- GPCRsSIGNAL TRANSDUCTION

GPCRs

� GPCRs (G-Protein Coupled Receptors) -Histamine Receptors


PR-611 gpH1R 1 ml

(Histamine H1-Receptor) Guinea pig

PR-612 gpH1R + RGS4 1 ml

(Histamine H1-Receptor + Regulator of

G-protein Signaling 4) Guinea pig

PR-613 gpH2R 1 ml

(Histamine H2-Receptor) Guinea pig

PR-614 hH1R 1 ml

(Histamine H1-Receptor) Human

PR-615 hH1R + RGS4 1 ml

(Histamine H1-Receptor + Regulator of

G-protein Signaling 4) Human

PR-616 hH2R 1 ml

(Histamine H2-Receptor) Human

PR-617 hH2R-G

qααααα 1 ml(Histamine H

2-Receptor G

qα fusion protein)Human

PR-618 hH2R-G

sαααααL1 ml

(Histamine H2-Receptor G

sαL fusion protein)

Human

PR-619 hH2R-G

sαααααS1 ml

(Histamine H2-Receptor G

sαS

fusion protein) Human

PR-620 rH2R 1 ml

(Histamine H2-Receptor) Rat

PR-621 hH2R + G

sαααααS1 ml

(Histamine H2-Receptor + G

sαS) Human

� GPCRs (G-Protein Coupled Receptors) -Leukotriene Receptors

The human leukotriene B4 receptor (BLTR) is a seven transmembrane-domain (7TM), G protein-coupled receptor (GPCR). BLTR reconsti-tuted with the G-protein Giα

1β

1γ

2 was prepared from Sf9 cells triple-

infected with BLTR-, Giα1-, and Gα

1α

2-encoding baculoviruses.

BLTR reconstituted with the G-protein Giα2β

1γ

2 was prepared from Sf9

cells triple-infected with BLTR-, Giα2-, and Gβ

1γ


BLTR reconstituted with the G-protein Giα2β

1γ


cells triple-infected with BLTR-, Giα3-, and Gβ1γ



PR-560 BLTR + Gia1

βββββ1γγγγγ

21 ml

(Leukotriene B4 Receptor + inhibitory

G-protein Gia1

β1γ

2)

Human

PR-561 BLTR + Gia2


21 ml


G-protein Gia2

β1γ

2) Human

PR-562 BLTR + Gia3


21 ml


G-protein Gia3

β1γ

2) Human

� GPCRs (G-Protein Coupled Receptors) -Dopamine Receptors

The dopamine D1-receptor (D

1R) belongs to the superfamily of seven

transmembrane-domain (7TM), G-protein-coupled receptors (GPCRs).The D

1R couples to the G-protein Gs to mediate adenylyl cyclase (AC)

activation. The endogenous catecholamine dopamine influences manycellular activities including behavior, hormone synthesis, blood pressurecontrol and intracellular ion transport. The human D

1R was prepared

from Sf9 cells infected with D1R-encoding baculovirus. In this system the

D1R couples to the Gsα-like G-proteins of the insect cells to mediate ACactivation.

� GPCRs (G-Protein Coupled Receptors) -Formyl Peptide Receptors

The formyl peptide receptor (FPR) is expressed in neutrophils, couplesto pertussis toxin (PTX)-sensitive Gi-proteins and activates phospholi-pase C, chemotaxis and cytotoxic cell functions. The FPR, like mostGPCRs, is a type IIIb membrane protein with an extracellular N-terminuswithout a signal sequence. The human FPR exists in various isoforms,FPR-26, FPR-98 and FPR-G6 respectively. These FPR isoforms differfrom each other in amino acid positions 101 (localized at the top of thethird transmembrane domain), 192 (localized in the center of the sec-ond extracellular loop) and 346 (localized at the extreme C-terminus).

FPR-26/FPR-98/FPRG6 is reconstituted with the G-protein Giα2β

1γ

2 is

constitutively active and was prepared from Sf9 cells triple-infectedwith FPR-, Giα

2-, and Gβ

1γ


The human formyl peptide receptor (FPR) is N-glycosylated and activatesphagocytes via Gi-proteins. The FPR possesses two potentialN-glycosylation sites in the N-terminus (Asn4 and Asn10) and one potentialN-glycosylation site in the second extracellular loop (Asn179). FPR-NGIIlacks the N-glycosylation sites at Asn4 and Asn10. FPR-NGII reconstitutedwith the G-protein Giα

2β

1γ

2 was prepared from Sf9 cells triple-infected

with FPR-, Giα2-, and Gβ

1γ

2-encoding baculoviruses. Compared to wildtype

FPR, FPR-NGII is much less active in terms of high-affinity agonist bindingand agonist-stimulated [35S]GTPgS binding.


PR-581 Dopamine Receptor D1R 1 ml

Human


PR-591 FPR26 + Giααααα2


21 ml

(Formyl Peptide Receptor +inhibitory G-protein G

iα2β

1γ

2) Human

PR-592 FPR98 + Giααααα2


21 ml

(Formyl Peptide Receptor + inhibitoryG-protein G

iα2β

1γ

2) Human

PR-593 FPRG6 + Giααααα2


21 ml

(Formyl Peptide Receptor +inhibitory G-protein G

iα2β

1γ

2)


PR-594 FPR-NGII + Giααααα2


21 ml

(Formyl Peptide Receptor,non-glycosylated, + inhibitoryG-protein G

iα2β

1γ

2)



GTP Family- GPCRs, ImportinsSIGNAL TRANSDUCTION

GPCRs, Importins

� GPCRs (G-Protein Coupled Receptors) -Complement Component 5a Receptors

The anaphylatoxin C5a-receptor (C5aR, CD88) belongs to the superfamilyof seven transmembrane-domain (7TM), G protein-coupled receptors(GPCRs). In monocytes and granulocytes, activation of C5aR causes chemo-taxis, degranulation, and superoxide anion production. C5aR is also in-volved in the formation of adhesion molecules as well as in the productionof so-called “acute-phase” proteins.

C5aR reconstituted with the G-protein Giα1

β1γ


cells triple-infected with C5aR-, Giα1-, and Gβ1γ



PR-571 C5aR + Giααααα1


21 ml

(Complement Component 5a Receptor +inhibitory G-protein G

iα1β

1γ

2)




21 ml


iα2β

1γ

2)




21 ml


iα3β

1γ

2)


� GPCRs (G-Protein Coupled Receptors) -Platelet-activating Factor Receptors

Platelet-activating factor (PAF) is a pro-inflammatory lipid mediatorpossessing a unique 1-O-alkyl-2-acetylsn- glycero-3-phosphocholine back-bone. The PAF receptor (PAFR), which belongs to the superfamily ofseven transmembrane (7TM), G-proteincoupled receptors (GPCRs),transduces pleiotropic functions including cell motility, smooth musclecontraction, and synthesis and release of mediators and cytokines viamultiple heterotrimeric G-proteins.

PAFR is reconstituted with the G-protein Giα1

β1γ


cells triple-infected with PAFR-, Giα1

-, and Gβ1γ



PR-631 PAFR + Giααααα1


21 ml

(Platelet Activating Factor Receptor +inhibitory G-protein G

iα1β

1γ

2)


PR-632 PAFR + Giααααα2


21 ml


iα2β

1γ

2)


PR-633 PAFR + Gia3


21 ml


ia3β

1γ

2)


� GPCRs (G-Protein Coupled Receptors) -Cell Extracts

This membrane preparation was made from uninfected Sf9 insect cellsand can be used as a negative control for immunoblotting. Specifically,membranes from uninfected Sf9 cells do not show immunoreactivity withthe M1-antibody (reacting with the FLAG epitope) and commonly usedantibodies against mammalian Gsα, Giα and Gqα. Membranes fromuninfected Sf9 cells exhibit low but clearly detecable adenylate cyclaseactivity that can be used as background to assess the effects of exog-enously expressed receptors and G-proteins.


PR-634 Membrane preparation 1 ml(Sf9 cell extract) uninfected, Sf9 insect cells

� Importins

Importins are involved in nuclear import and belong to a seperate classof Ran-GTP binding proteins. Importin α itself does not interact withnuclear pore complex (NPC) but functions as an adaptor that bindsImportin β. Both these importins inhibit mitotic spindle assembly.

Importin β binds the importin α-NLS complex or other adaptors to medi-ate the uptake of the substrateimportin a/b complex through the nuclearpore complex. Importin α and β inhibit mitotic spindle assembly.


PR-231 Importin αααααHis 50 µgHuman

PR-232 Importin βββββHis 50 µgD. melanogaster


PI3 Kinase FamilySIGNAL TRANSDUCTION

P13K

� Phosphoinositide 3-kinase (PI3K) isoforms –native proteins, affinity-tagged variantsand associated products

Phosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols(PIs) at their 3´-OH position generating lipid second messengers andthereby regulate numerous biological processes including cell growth,differentiation, survival, proliferation, migration and metabolism. Onthe basis of structural similarities and substrate specificity, the PI3Kfamily can be subdivided into three classes termed I, II, and III.

� PI3KαααααPI3Ka plays a specific role in apoptosis in human colon cancer cells.Injection of neutralizing antibodies specific to PI3Kα into adenocarcinomacells induced apoptosis, a response that was reverted by treating cellswith caspase inhibitor. It was also shown that PI3Kα mediatedphosphorylation of the p85a adapter reduces the lipid kinase activity ofthe heterodimer and this gives hints for PI3K-dependent signaling eventsnot requiring production of 3’-phosphorylated phosphoinositides. PI3Kαis a key regulator of the initiation of keratinocyte differentiation. Adecrease in PI3Kα activity results in a loss of keratinocyte adhesion tothe extracellular membrane and the initiation of early phasedifferentiation.

The PI3Kα catalytic and regulatory subunits are coexpressed in Sf9 in-sect cells.


PR-341S PI(3)Kααααα, S pack 5 µg(Phosphoinositide 3-Kinase a, p110ααααα/p85ααααα)Bovine, Recombinant, Sf9 insect cells

PR-341L PI(3)Kααααα, L pack 10 µg(Phosphoinositide 3-Kinase α, p110α/p85α)Bovine, Recombinant, St9 insect cells

PR-940S PI(3)KαααααGST, S pack 5 µg(Phosphoinositide 3-kinase p110α/p85α)Bovine, Recombinant, Sf9 insect cells

PR-940L PI(3)KαααααGST, L pack 10 µg(Phosphoinositide 3-kinase p110α/p85α)Bovine, Recombinant, Sf9 insect cells

PR-941S PI(3)KαR916PGST, S pack 5 µg(Phosphoinositide 3-kinase p110α, p85α)Bovine, Recombinant, Sf9 insect cells

PR-941L PI(3)KαααααR916PGST, L pack 10 µg(Phosphoinositide 3-kinase p110α, p85α)Bovine, Recombinant, Sf9 insect cells

PR-342 p85ααααα 20 µg(Phosphoinositide 3-Kinase α, regulatorysubunit) Bovine, Recombinant,

� PI3KβββββThe PI3Kβ isoform can be activated by insulin via the insulin receptor toinitiate a cascade of events that control cell growth and metabolism. Theactivation of PI3Kβ is mediated by the p85 regulatory subunit binding totyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g.IRS-1 and IRS-2). It was also shown that PI3Kβ is involved in apoptosis inhuman colon carcinoma cells. Injection of neutralizing antibodies spe-cific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhib-ited de novo DNA synthesis. PI3Kβ is the major PI3K isoform requiredfor apoptotic cell and Fc-g receptor mediated phagocytosis shown forprimary mouse macrophages and the Jurkat human leukemia T cell line.It was shown by several research groups that the catalytic subunit ofPI3Kβ can be activated by Gβγ.

� PI3Kγγγγγ

PI3Kγ is highly expressed in cells of hematopoietic origin. It plays animportant role in dendritic cell (DC) trafficking and in the activation ofspecific immunity. PI3Kγ-/- mice showed a reduced ability to respond tochemokines, and had a selective defect in the number of skin Langerhanscells and in lymph node CD8a-DCs. In macrophages, the chemokineRANTES/CCL5 activates the small GTPase Rac1 and its downstream tar-get PAK2.

The PI3KγD946A protein is a catalytically inactive mutant of PI3Kγ with aD946A mutation in the ATP binding site. This recombinant catalyticallyinactive protein can be used as a negative control in any kind of PI3Kgkinase activity studies.Recombinant full length PI3KγDA mutant carries a

N-terminal GST-Tag and was purified by affinity chromatography.

Distinct regions within the p101 regulatory subunit primary stucture areresponsible for interaction with PI3Kγ and Gβγ. The PI3Kγ binding site isconfined to the N-terminus, whereas binding to Gbγ is mediated by theC-terminus of p101. The PI3Kγ catalytic and regulatory subunits arecoexpressed in Sf9 insect cells. The PI3Kγ catalytic subunit carries aN-terminal His6 affinity Tag, whereas the p101 adaptor subunit carries aN-terminal GST-Tag.


PR-343S PI(3)KγγγγγHis, S pack 5 µg(Phosphoinositide 3-Kinase γ, p110γγγγγ)Human, Recombinant, Sf9 insect cells

PR-343L PI(3)KγγγγγHis, L pack 10 µg(Phosphoinositide 3-Kinase γγγγγ, p110γγγγγ)Human, Recombinant, Sf9 insect cells

PR-346S PI(3)KγγγγγD946AGST, S pack 5 µg(Phosphoinositide 3-kinase γγγγγ, p110γγγγγ,catalytically inactive mutant)Human, Recombinant, Sf9 insect cells

PR-346L PI(3)KγγγγγD946AGST, L pack 10 µg(Phosphoinositide 3-kinase γ, p110γ,catalytically inactive mutant)Human, Recombinant, Sf9 insect cells

PR-347S PI(3)KγγγγγHis/p101GST, S pack 5 µg(Phosphoinositide 3-Kinase γ, p110γ/p101)Human, Recombinant, Sf9 insect cells

PR-347L PI(3)KγγγγγHis/p101GST, L pack 10 µg(Phosphoinositide 3-Kinase γ, p110γ/p101)Human, Recombinant, Sf9 insect cells


PR-344S PI(3)Kβββββ, S pack 5 µg(Phosphoinositide 3-Kinase βββββ, p110βββββ/p85α)Human

PR-344L PI(3)Kβββββ, L pack 10 µg(Phosphoinositide 3-Kinase β, p110β/p85α)Human


PI3 Kinase FamilySIGNAL TRANSDUCTION

PI3K

� PI3KδδδδδRecently it was shown that the inactivation of PI3Kδ in bone marrow mastcells (BBMCs) leads to defective stem cell factor-mediated proliferation,adhesion and migration of these cells, and to impaired allergen-IgEinduceddegranulation and cytokine release. In neutrophils a role for PI3Kδ inTNFα-induced signalling was demonstrated by a reduction inAktphosphorylation and PDK1 activity upon treatment with the δ-specificinhibitor IC87114.

The PI3Kδ catalytic and regulatory subunits are coexpressed in Sf9 in-sect cells. The catalytic subunit carries a GST-Tag and the heterodimerwas purified by affinity chromatography.

� PI3 Kinase Family - Adaptor Proteins

p85α functions as the regulatory subunit of the class IA PI3-kinase isoformsα, β, and δ. It contains two SH2 domains that bind to tyrosine-phospho-rylated growth factor receptors or substrate adaptor proteins. It alsocontains a BH (breakpoint cluster region homology) domain that showsGAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and toa lesser extend towards Rab6 and Rab11. It was shown that PI3Kα cata-lytic subunit mediated phosphorylation of the p85α adapter reduces thelipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3’-phosphory-lated phosphoinositides. PI3Kα protein kinase activity has been impli-cated in IRS-1 serine phosphorylation in insulin-treated adipocytes andin STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFNreceptor by IFN-α.


PR-345S PI(3)KδδδδδGST, S pack 5 µg(Phosphoinositide 3-Kinase δ, p110δ/p85α)Human

PR-345L PI(3)KδδδδδGST, L pack 10 µg(Phosphoinositide 3-Kinase δδδδδ, p110δδδδδ/p85α)Human


PR-342 p85ααααα 20 µg(Phosphoinositide 3-Kinase α,regulatory subunit)Bovine, Recombinant, Leishmania tarentolae

� PI3 Kinase Family – Inhibitors


INH-001 Wortmannin 1 mg(PI3-Kinase Inhibitor)Talaromyces wortmannin KY 12420

INH-002 LY294002 1 mg(PI3-Kinase Inhibitor)

INH-003 Quercetin 100 mg(Protein Kinase Inhibitor)

INH-004 Myricetin 5 mg(Protein Kinase Inhibitor)

INH-005 Staurosporine 50 mg(Protein Kinase Inhibitor)Streptomyces staurosporeus

� PI3 Kinase Family – Substrates


LI-011 PI3K Lipid Kinase Substrate Mix 1 1 mg (based(PI, PE, PS, PC, and SM) on PI)

LI-012 PI3K Lipid Kinase Substrate Mix 2 0.1 mg(PIP

2, PE, PS, PC, and SM) (based on PIP

2)

LI-001 PI 5 mg(L-α-Phosphatidylinositol)Bovine liver, sodium salt

LI-008 PI 100 µg[L-α-Phosphatidylinositol-(1,2-dipalmitoyl)-D-myo-inositol]synthetic

LI-002 PI-4-P 1 mg(L-α-Phosphatidylinositol-4-phosphate)Porcine brain, diammonium salt

LI-009 PI-4-P 100 µg[L-α-Phosphatidylinositol-(1,2-dipalmitoyl)-D-myo-inositol 4-monophosphate]synthetic

LI-003 PI-4,5-P2

0.5 mg(L-α-Phosphatidylinositol-4,5-bisphosphate) Porcine brain,triammonium salt

LI-010 PI-4,5-P2

100 µg[L-α-Phosphatidylinositol-(1,2-dipalmitoyl)-D-myo-inositol 4,5-bisphosphate]synthetic

LI-004 PC 5 mg(L-α-Phosphatidylcholine)Chicken egg

LI-005 PE 5 mg(L-α-Phosphatidylethanolamine)Chicken egg

LI-006 PS 5 mg(L-α-Phosphatidylserine)Porcine brain, sodium salt

LI-007 SM 5 mg(Sphingomyelin)Porcine brain


ABD-026S ααααα-hPI(3)Kγγγγγ (mAb p110γγγγγ) WB 200 µl(anti-human Phosphoinositide 3-kinase gamma)mouse monoclonal antibody, IgG2a, clone H1

ABD-026L ααααα-hPI(3)Kγγγγγ (mAb p110γγγγγ) WB 1 ml(anti-human Phosphoinositide 3-kinase gamma)mouse monoclonal antibody, IgG2a, clone H1

ABD-027 ααααα-hPI(3)Kγγγγγ (mAb p110γγγγγ) IP 1 ml(anti-human Phosphoinositide 3-kinase gamma)mouse monoclonal antibody, IgG2a, clone 641

ABD-027P ααααα-hPI(3)Kγγγγγ (mAb p110γγγγγ) IP 100 µg(anti-human Phosphoinositide 3-kinase gamma)mouse monoclonal antibody, IgG2a, clone 641

� PI3 Kinase Antibodies


Protein Kinases – Tyrosine & Ser/Thr KinasesSIGNAL TRANSDUCTION

PTK, PKA, PKB, PKC

� Tyrosine Kinases (Contd.)

N-terminal GST tagged Abl SH3 domain. Abl SH3 domain is the SH3 domainof Abl tyrosine kinase. SH3 domains are known to bind proline rich pep-tides. The Abl SH3 domain has been expressed as a fusion protein withGST allowing expression in E. coli and purification by affinity chroma-tography.

N-terminal GST tagged Hck SH3 domain. Hck SH3 domain is the SH3domain of Hck tyrosine kinase. SH3 domains are known to bind prolinerich peptides. The Hck SH3 domain has been expressed as a fusion pro-tein with GST allowing expression in E. coli and purification by affinitychromatography.

Src (p60c-src) is a 60 kDa cytoplasmic tyrosine kinase with functions inthe regulation of cell division, cell-matrix adhesion, and cell-cellcell ad-hesion. Src family tyrosine kinases participate in signal transduction ofdifferent classes of membrane receptors, including receptor tyrosinekinases and G-protein coupled receptors.

N-terminal GST tagged Src SH3 domain. Src SH3 domain is the SH3 do-main of Src tyrosine kinase. SH3 protein with GST allowing expression inE. coli and purification by affinity chromatography.

Platelet-derived growth factors (PDGF) are potent mitogens for cells ofmesenchymal origin, including smooth muscle cells and glial cells. ThePDGF isoforms AA, BB, AB, CC, and DD are dimeric molecules. They bindtwo receptor molecules simultaneously and dimerize them upon binding.The autophosphorylation induced after dimerization of PDGF receptorsserves two important functions. On one hand, phosphorylation of a con-served tyrosine residue inside the kinase domains (Tyr-849 in the β-receptor and Tyr-857 in the α-receptor) leads to an increase in thecatalytic efficiencies of the kinase. On the other hand,autophosphorylation of tyrosine residues located outside the kinase do-main creates docking sites for signal transduction molecules containingSH2 domains.

� Protein A, B and C Kinases

The principle intracellular target for cAMP in mammalian cells is thecAMP-dependent protein kinase (PKA). A large number of hormones,neurotransmitters and other signal substances utilize adenosine 3’, 5’cyclic monophosphate (cAMP) as an intracellular second messenger. Therecombinant human PKA, catalytic subunit alpha was expressed in E. coli

and purified by Ni-agarose chromatography. It is suitable for labelingPKA substrates. The sequence based calculated molecular weight is 42.3kDa.

Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which inmammals comprises three highly homologous members known asPKBα(Akt1), PKBβ(Akt2), and PKBγ(Akt3). PKB/Akt is activated in cellsexposed to diverse stimuli such as hormones, growth factors, and extra-cellular matrix components. The Thr-308 residue in the kinase domainand Ser-473 residue in the tail domain of Akt1 need to be phosphorylatedby PDK1 and PDK2, respectively, for its maximal activation. The activa-tion mechanism remains to be fully characterised but occurs downstreamof PI-3K. Akt1 contains a region homologous to a pleckstrin domain foundin multiple signaling molecules and is stimulated by a number of receptortyrosine kinases, including receptors for IGF, NGF, PDGF, VEGF, angio-tensin, and insulin, by the action of PI 3-kinase. The recombinant humanAkt1 kinase (PKBα) is expressed in Sf9 insect cells and purified by Ni-NTAagarose chromatography. It is suitable for labeling Akt1 kinase substrates.The sequence based calculated molecular weight is 59.05 kDa.

� Serine/Threonine Kinases

MKK6 is one of the major specific activators of p38. MAP kinase kinases(MAPKKs) MKK3 and MKK6 are the primary regulators of p38 phosphory-lation and activation. The MKK6/p38 pathway is well documented in theregulation of apoptosis. The p38 mitogen-activated protein (MAP) ki-nase signal transduction pathway regulates the production of interleukin-1 and tumor necrosis factor-alpha. The recombinant human MKK6 wasexpressed in E. coli and purified by glutathione sepharose chromatogra-phy.

MAP (mitogen-activated protein) kinases are activated by a family ofdual specificity kinases called MEKs (MAP kinase/Erk Kinase). MEK1 canbe activated by Raf by phosphorylation on serine 218 and serine 222.Mutation of these sites to acidic residues leads to constitutively activeMEK1 in some cases. Given the central role of the ERK/mitogen-activatedprotein kinase pathway in mediating growth-promoting signals for adiverse group of upstream stimuli, inhibitors of MEK, as a key centralmediator, could have significant clinical benefit in the treatment ofbreast and other cancers. The human recombinant MEK1, activated bytwo amino acid exchanges (S218D, S222D), was expressed in E. coli andpurified by gluthatione sepharose and gelfiltration. It is suitable forlabeling MEK1 substrates and for activation of ERK1 and ERK2. The mo-lecular weight of the protein is 69.7 kDa.


PR-305 RafRBD (c-Raf)(Ras Binding Domain of Raf, 50 µgc-Raf 1 Kinase)

PR-366 RafRBDGST (c-Raf)(Ras Binding Domain 50 µgof Raf, c-Raf 1 Kinase)


PR-321 MKK6GST, active(Mitogen- 20 µgactivated protein kinase kinase)

PR-161 MEK1GST, active(Mitogen-activated 10 µgkinase kinase 1, MAPKK1)

� Protein Kinases - Tyrosine Like Kinases

Raf is a member of the serine/threonine proteine kinase family involvedin regulation of cell growth and differentiation and is the most importanteffector of Ras. Full-length Raf is composed of three conserved regionsresponsible for interaction with Ras, for phosphorylation and for cata-lytic activity. RafRBD (Ras binding domain, amino acids 50-132) medi-ates interaction with membrane-anchored Ras necessary for activationof the kinase activity of Raf.


PR-337 AblGST SH3 domain(Abelson Tyrosine Kinase, SH3 Domain) 50 µg

PR-336 HckGST SH3 domain 50 µg(Hematopoietic Tyrosine Kinase, SH3 domain)

PR-339 LckGST SH3 domain 50 µg(Lymphocyte-specific Kinase, SH3 Domain)

PR-900 SrcGST 20 µg(Sarcoma inducing protein kinase)

PR-338 SrcGST SH3 domain 50 µg(Sarcoma inducing tyrosine kinase,SH3 domain)

PR-357 PDGFR-βββββGST, cytosolic region 20 µg(Platelet-derived growth factor receptor)


Protein Kinases – PKA, PKB, PKC, CamK, Cyclin dependentSIGNAL TRANSDUCTION

PKA, PKB, PKC, CamK, Cyclin dep.

� Protein A, B and C Kinases

Protein kinase C alpha (PKC alpha) is a serine/threonine kinase and amember of the conventional (classical) PKCs (cPKCs), which have fourconserved (C1 to C4) regions. This ubiquitously expressed PKC isotype isactivated in response to many different kinds of stimuli and translocatesfrom cytosol to the specialized cellular compartments (nucleus, focaladhesion, caveolae, etc.) where it is presumed to work. Therefore, PKCalpha has been implicated in a variety of cellular functions includingproliferation, apoptosis, differentiation, motility, and inflammation.The recombinant human PKCαwas expressed in E coli and purified by Ni-agarose chromatography. It is suitable for labeling PKC substrates. Thesequence based calculated molecular weight is 81.9 kDa.

� Calcium/Calmodulin-dependent Kinases

Checkpoint kinase 2 (CHK2) is emerging as a key mediator of diversecellular responses to genotoxic stress, guarding the integrity of thegenome throughout eukaryotic evolution. Recent studies show the funda-mental role of CHK2 in the network of genome surveillance pathways thatcoordinate cell-cycle progression with DNA repair and cell survival ordeath. Defects in CHK2 contribute to the development of both heredi-tary and sporadic human cancers, and earmark

this kinase as a candidate tumour suppressor and an attractive targetfor drug discovery. The recombinant human CHK2 was expressed in Sf9insect cells and purified by Ni-NTA agarose chromatography. It is suit-able for labeling CHK substrates. The sequence based calculated molecu-lar weight is 66.06 kDa.


PR-313 CHK2His, active 20 µg(Checkpoint Kinase 2)


PR-318 PKAHis, ααααα-subunit, active 50 µg(Protein Kinase A)

PR-324 Akt1/ PKBαααααHis, active 20 µg(Protein Kinase B)

PR-325 Akt1/ PKBαααααHis, inactive 20 µg(Protein Kinase B)

PR-333 Akt2/PKBβββββHis, active 100 µg(Protein kinase B)

PR-334 Akt3/ PKBγγγγγ His, active 20 µg(Protein Kinase B)

PR-329 PKCαααααHis, active 10 µg(Protein Kinase C)

� Cyclin-dependent Kinases

Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associ-ated serine/threonine protein kinase. Similar to protein kinase A, CK2 isa tetramer made up from two different subunits α

2β

2. The catalytic α-

subunit corresponds to the C-subunit of PKA, the non-catalytic β-subunitis unique and differs from the R-subunit of PKA in all known features.

The recombinant human CK2 subunits α and β were expressed separatelyas non-fusion proteins in E. coli and reconstituted to the α

2β

2 holoenzyme

in the course of the purification using several chromatography steps.The protein is suitable for labeling CK2 substrates. The molecular weightis 140 kDa.

The human recombinant protein kinase CK2α1 was expressed in E. coli asa non-fusion protein and purified by several chromatography steps. Theα-subunit is constitutively active and suitable for labeling casein kinase 2alpha substrates.

The human recombinant protein kinase CK2α2 was expressed in E. coli asa N-MBP fusion protein. The α-subunit is constitutively active and suit-able for labeling casein kinase 2 alpha substrates. The molecular weightof the protein is 84.3 kDa.

JNK2α2/SAPK1a belongs to the family of mitogenactivated protein ki-nases (MAPK). Mitogen-activated protein (MAP) kinases comprise a familyof ubiquitous proline-directed, protein serine/ threonine kinases, whichparticipate in signal transduction pathways that control intracellularevents including acute responses to hormones and major developmentalchanges in organisms.

p38α/SAPK2a belongs to the family of mitogen-activated protein kinases(MAPK).

Mitogen-activated protein (MAP) kinases comprise a family of ubiquitousproline-directed, proteinserine/ threonine kinases, which participatein signal transduction pathways that control intracellular events includingacute responses to hormones and major developmental changes inorganisms. The recombinant human p38αkinase (SAPK2a) was expressedin E. coli and purified by Ni-agarose chromatography. The active formwas produced by phosphorylation of the purified p38αwith MKK6. It issuitable for labeling p38αkinase substrates. The sequence based calculatedmolecular weight is 42.7 kDa.

MAPK families play an important role in complex cellular programs likeproliferation, differentiation, development, transformation, andapoptosis. At least three MAPK families have been characterized: extra-cellular signalregulated kinase (ERK), Jun kinase (JNK/SAPK) and p38MAPK.

Activated ERK can enter the nucleus and phosphorylate transcriptionfactors providing the link between cell surface receptor-mediated eventsand nuclear induction of gene expression. In the nucleus activated ERKpromotes the transcription and the activity of transcription factors c-fos, c-myc, c-jun and p21. The recombinant human ERK2 was expressedin E. coli and purified by Ni-agarose chromatography. It is suitable forlabeling ERK2/MAPK2 substrates. The highly active form is produced byphosphorylation of the protein in vitro with MEK1. The sequence basedcalculated molecular weight is 44.56 kDa.

The recombinant human ERK1 was expressed in E. coli and purified byNi-agarose chromatography. It is suitable for labeling ERK1 substrates.The highly active form is produced by phosphorylation of the protein invitro with MEK1. The sequence based calculated molecular weight is43.62 kDa.


PR-316 CK2, holoenzyme, active 10 µg(Casein Kinase 2, α- and β-subunit)


Protein Kinases – Cyclin dependent, Substrates, InhibitorsSIGNAL TRANSDUCTION

Cyclin dep. Kinase, substrate, Inhibitors


PR-314 CK2ααααα1, active 10 µg(Casein Kinase 2, ααααα-subunit)

PR-160 CK2ααααα2MBP, active 10 µg(Casein Kinase 2, ααααα-prime subunit)

PR-317 CK2ααααα, active 10 µg(Casein Kinase 2, ααααα-subunit)

PR-315 CK2βββββ 10 µg(Casein Kinase 2, βββββ-subunit)

PR-326 JNK2ααααα2/ SAPK1aHis, active 100 µg(c-jun kinase/ stress-activated protein kinase)

PR-327 p38ααααα/ SAPK2aHis, active 20 µg(Stress-activated protein kinase)

PR-328 p38ααααα/ SAPK2aHis, inactive 20 µg(Stress-activated protein kinase)

PR-331 p42/ ERK2/ MAPK2His, active 5 µg(Extracellular signal-regulated kinase/mitogen-activated protein kinase)

PR-332 p42/ ERK2/ MAPK2His, inactive 10 µg(Extracellular signal-regulated kinase/mitogen-activated protein kinase)

PR-320 p42/ ERK2/ MAPK2His 50 µg(Extracellular signal-regulated Kinase)Rat, Recombinant, E. coli

PR-322 p44/ERK1His, active 10 µg(Extracellular signal-regulated kinase/mitogen-activated protein kinase)

PR-323 p44/ERK1His, inactive 20 µg(Extracellular signal-regulated kinase/mitogen-activated protein kinase)

� Cyclin-dependent Kinases (Contd.)


INH-003 Quercetin 100 mg(Protein Kinase Inhibitor)

INH-004 Myricetin 5 mg(Protein Kinase Inhibitor)

INH-005 Staurosporine 50 µg(Protein Kinase Inhibitor)Streptomyces staurosporeus

INH-006 SP600125 2 mg(JNK Inhibitor)

INH-007 SB203580 2 mg(p38 MAPK Inhibitor)

INH-008 SB202190 2 mg(p38 MAPK Inhibitor)

� Inhibitors


PE-200 Akt substrate peptide 1 mgRPRAATF

PE-201 Abl substrate peptide 1 mgEAIYAAPFAKKK

PE-203 Cdc2 substrate peptide 1 mg(Ac-S)PGRRRRK

PE-204 Cdk5 substrate peptide 1 mgPKTPKKAKKL

PE-205 CHK substrate peptide 1 mgKKKVSRSGLYRSPSMPENLNRPR

PE-206 CK1 substrate peptide 1 mgRRKDLHDDEEDEAMSITA

PE-207 CK2 substrate peptide 1 1 mgRRRDDDSDDD

PE-213 CK2 substrate peptide 2 1 mgRRRADDSDDDDD

PE-202 GSK3 substrate peptide 1 mgYRRAAVPPSPSLSRHSSPHQ(pS)EDEEE

PE-208 PKA substrate peptide 1 mgGRTGRRNSI

PE-209 PKC substrate peptide 1 mgQKRPSQRSKYL

Src substrate peptide 1 mgKVEKIGEGTYGVVYK

PE-200 AMPK substrate peptide 1 mgHMRSAMSGLHLVKRR

PE-201 DYRK substrate peptide 1 mgKKISGRLSPIMTEQ

PE-203 LKB1 substrate peptide 1 mgLSNLYHQGKFLQTFCGSPLYRRR

� Substrate Peptides

� PKA Substrates


60510-1 CREBtide Native KRREILSRRPSYR 1 mg(Also available in Fluor &Biotin derivatives)

22593 Kemptide Native LRRASLG 1 mg

60528-1 Kemptide 1 mgPhosphorylated LRRAS(PO3)LG(Also available in Fluor &Biotin derivatives)

� PKA, PKC, MAPKAP-K1 Substrates


29983-1 Native RRRLSSLRA-NH2 1 mg(Also available in Fluor &Biotin derivatives)


Substrates for Protein KinasesSIGNAL TRANSDUCTION

Kinase Substrates

� PKC Substrates


27165 MARCKS Protein (154-165) Native 1 mgKKRFSFKKSFKL(Also available in Fluor & Biotin derivatives)

27173 Neurogranin 28-43 Native 1 mgAAKIQASFRGHMARKK

29994-1 Neurogranin 28-43 Phosphorylated 1 mgAAKIQAS(PO3)FRGHMARKK(Also available in Fluor & Biotin derivatives)

27177 Glycogen Synthase residues 1-8 1 mgNative PLSRTLSVAAKK-NH2(Also available in Fluor & Biotin derivatives)

60542-1 RRGRTGRGRRGIFR (Native) 1 mg(Also available in Fluor & Biotin derivatives)

60546-1 RRGRTGRGRRGIFR Phosphorylated 1 mg

60560-1 Native RFAVRDMRQTVAVGVIKAVDKK 1 mg

60535-1 Phosphorylated 1 mgRFAVRDMRQT(PO3)VAVGVIKAVDKK(Also available in Fluor & Biotin derivatives)

� PKG Substrates


60506-1 BPDEtide [RKISASEFDRPLR] 1 mgNative RKISASEFDRPLR(Also available in Fluor &Biotin derivatives)

27185 Native QKRPRRKDTP 1 mg

29997-1 Phosphorylated QKRPRRKDT(PO3)P 1 mg(Also available in Fluor &Biotin derivatives)

60298-1 Native RKRSRAE 1 mg

60302-1 Phosphorylated RKRS(PO3)RAE 1 mg(Also available in Fluor &Biotin derivatives)

� Substrates for CaM Kinase II


29954-1 Residues 281-291 of CamK II 1 mg(autophophorylation site) NativeMHRQETVDCLK-NH2

29958-1 Residues 281-291 of CamK II 1 mgPhosphorylatedMHRQET(PO3)VDCLK-NH2(Also available in Fluor & Biotin derivatives)

27177 Glycogen Synthase residues 1-8 1 mgNative PLSRTLSVAAKK-NH2(Also available in Fluor &Biotin derivatives)

60514-1 Glycogen Synthase residues 1-10 Native 1 mgPLSRTLSVSS-NH2(Also available in Fluor &Biotin derivatives)

22511 Syntide-2 Native 1 mgPLARTLSVAGLPGKK(Also available in Fluor &Biotin derivatives)

� Substrate for AKT/PKB


60209-1 Crosstide [GRPRTSSFAEG] Native 1 mg(Also available in Fluor & Biotin derivatives)

29944-1 ARKRERTYSFGHHA Native 1 mg(Also available in Fluor & Biotin derivatives)

29946-1 ARKRERTYS(PO3) FGHHA 1 mgPhoshphorylated

� Substrates for Enzyme CaMK


60249-1 Autocamtide-2 Native 1 mgKKALRRQETVDAL

29929-1 Autocamtide-2 Phosphorylated 1 mgKKALRRQET(PO3)VDAL (Also available in Fluor & Biotin derivatives)

29973-1 Autocamtide-3 Native 1 mgKKALHRQETVDAL

29974-1 Autocamtide-3 Phosphorylated 1 mgKKALHRQET(PO3)VDAL(Also available in Fluor & Biotin derivatives)

� PKCε ε ε ε ε Substrate


27183 Native ERMRPRKRQGSVRRRV 1 mg

60533-1 Phosphorylated ERMRPRKRQGS(PO3)VRRRV 1 mg(Also available in Fluor & Biotin derivatives)

� PKCµ µ µ µ µ Substrates


29924-1 Native AALVRQMSVAFFFK 1 mg

29928-1 Phosphorylated AALVRQMS(PO3)VAFFFK 1 mg(Also available in Fluor & Biotin derivatives)


Substrate for Protein Kinases & PhosphatasesSIGNAL TRANSDUCTION

Kinase Substrates

� Substrates for MAPKs


27168 Native APRTPGGRR 1 mgSubstrate fopr p44 MAPK (ERK1) &p42 MAPK (ERK-2)

60746 Phosphorylated APRT(PO3)PGGRR 1 mg(Also available in Fluor &Biotin derivatives)

29969-1 EGF Receptor 661-681 Native 1 mgKRELVEPLTPSGEAPNQALLR-NH2

29965-1 EGF Receptor 661-681 Phosphorylated 1 mgKRELVEPLT(PO3)PSGEAPNQALLR-NH2(Also available in Fluor &Biotin derivatives)

60294-1 Tyrosine Hydroxylase 24-33 1 mgNative KQAEAVTSPR(Also available in Fluor & Biotin derivatives)

� Substrate for Calcineurin (PP2B)


24516 Phosphorylated 1 mgDLDVPIPGRFDRRVS(PO3)VAAE(Also available in Fluor &Biotin derivatives)

� Substrates for p60c-src


27197 Native YIYGSFK 1 mg(Also available in Fluor &Biotin derivatives)

� Substrate for p43 (cd2)


22579 Native 1 mgADAQHATPPKKKRKVEDPKDF(Also available in Fluor &Biotin derivatives)

29923-1 Phosphorylated 1 mgADAQHAT (PO3)PPKKKRKVEDPKDF

� Substrates for Protein Tyrosine phosphatases


24526 Protein Tyrosine Phosphatase 1 mgSubstrate I NativeENDY(PO3)INASL(Also available in Fluor &Biotin derivatives)

60512-1 MCA-EDAEY (PO3)AAK(DNP)R-NH2 1 mgFRET substrate for assaying PTPs

� Substrates for Protein Tyrosine Kinase


29942-1 Native ADEYLIPQQ 1 mg

29937-1 Phosphorylated ADEY(PO3)LIPQQ 1 mg(Also available in Fluor &Biotin derivatives)

60551-1 Tyrosine Kinase Peptide 1 1 mgKVEKIGEGTYGVVYK (Native)(Also available in Fluor &Biotin derivatives)

22581 Tyrosine Kinase Peptide 3 1 mgRRLIEDAEYAARG (Native)(Also available in Fluor &Biotin derivatives)

� Substrates for Protein Tyrosine phosphatase-1B


60634 Phosphorylated Ac-ELEFY(PO3)MDYE-NH2 1 mg(Also available in Fluor &Biotin derivatives)

24433 EGF Receptor Substrate 2 1 mgPhosphorylatedDADEY(PO3)LIPQQG(Also available in Fluor &Biotin derivatives)

29950-1 Native 1 mgELEFY(PO3)MDYE-NH2(Also available in Fluor &Biotin derivatives)

� Substrate for CDK5


60026-1 Native PKTPKKAKKL 1 mg(Also available in Fluor &Biotin derivatives)

29982-1 Phosphorylated 1 mgPKT (PO3)PKKAKKL

� Substrate for CDK1


60522-1 Native HATPPKKKRK 1 mg(Also available in Fluor &Biotin derivatives)

60526-1 Phosphorylated 1 mgHAT(PO3)PPKKKRK

� Substrates for Enzyme Casein Kinase


60537-1 Native RRADDSDDDDD 1 mgSubstrate for CKII

60541-1 Phosphorylated 1 mgRRADDS(PO3)DDDDD(Also available in Fluor &Biotin derivatives)

60547-1 Native RRKDLHDDEEDEAMSITA 1 mg(Also available in Fluor &Biotin derivatives)


PhosphatasesSIGNAL TRANSDUCTION

Phosphatases

� Recombinant Phosphatases

The dual specific Cdc25A (Cell division cycle) phosphatase regulates cellcycle progression, has oncogenic and anti-apoptotic activity, and isoverexpressed in many human tumors.

Protein Tyrosine Phosphatase type IVA, member 3, isoform 1; wholesequence. The proteins of regenerating liver (PRL-1, -2, and -3) areclosely related protein tyrosine phosphatases (PTPs) having high se-quence similarities to several dual-specificity PTPs. Elevated expressionlevels of PRL-3 have been reported in colon cancer cells that have metas-tasized to liver.

PTEN is one of the most frequently mutated tumor suppressors in humancancer. PTEN is a dual-specificity phosphatase with both protein phos-phatase and lipid phosphatase activity.

The best described substrate of PTEN is phosphatidylinositol (3,4,5)-tris-phosphate (PtdIns(3,4,5)P3). PTEN removes the phosphate inPtdIns(3,4,5)P3 to generate PtdIns(4,5)P2 and serves to counter-bal-ance the effects of PI3 Kinase. PTEN regulate the signaling pathway ofPI3 Kinases by preventing localisation of proteins with pleckstrin homol-ogy domains to the cell membrane. The molecular weight is about 48 kDa.

PTP 1B is the prototype non-transmembrane protein tyrosine phos-phatase. It is a ubiquitous protein that was originally identified in humanplacenta. This highly active enzyme is well suited for the study of ty-rosine phosphatase kinetics and substrate specificity, as well as for thescreening of inhibitors.

PTP-SL (PTPBR7) is a cytosolic membrane-associated enzyme with asingle catalytic domain. PTP-SL is a major regulator of extracellularsignal-regulated kinase ERK1/2 and p38. Interaction between PTP-SL andERK1/2 and p38 is strictly dependent on a kinase interaction motif (KIM)consisting of 16 amino acid residues.

PR-319 is is the catalytically inactive form of PTP-SL-ex, which wasobtained by site-directed mutagenesis of the active site cysteine toserine.

The intracellular region of PTPµ contains three domains: thejuxtamembrane (JM, aa 765-923) and two phosphatase domains (D1 andD2). Only the D1 domain is catalytically active.

Protein Tyrosin Phosphatase, cytoplasmic region, containing catalyticdomain D1 C-terminally linked to the non-catalytic domain D2. Molecularweight is 72.95 kDa.

SHP-1 is a protein-tyrosine phosphatase with tandem SH2 domains. It isa negative regulator of signaling of various receptors including the eryth-ropoietin receptor, the IL-3 receptor, c-Kit, the CSF-1 receptor, the B-cell receptor and c-Ros.

The mammalian dual-specificity protein-tyrosine phosphatase VHR (forVH1-related) has been identified as a novel regulator of extracellularregulated kinases (ERKs). Vaccinia Virus VH1-related Phosphatase (VHR),also known as Dual-Specificity Phosphatase 3 (DUSP3), removes phos-phate groups from tyrosine, serine, and threonine residues. It belongsto a family of phosphatases that selectively dephosphorylate MAP ki-nases. VHR has been shown to act as a phosphatase for several membersof the MAP kinase family including ERK1, ERK2, and JNK. It is a targetfor the ZAP-70 kinase, and phosphorylation of VHR at tyrosine 138 leadsto a downregulation of ERK2 activity.

Protein Tyrosine Phosphatase-Basophil-like (PTP-BL)is a large non-receptor type protein tyrosine phosphatase caracterized by the presenceof a extreme N-terminal KIND domain (kinase noncatalytic C-lobe domain),a N-terminal FERM domain (four point one, ezrin, radixin, moesin homologydomain), five PDZ (postsynaptic density protein-95, discs large, zonulaoccludens) regions and a C-terminal catalytically active domain.

� Phosphatase Assay Kit

Protein phosphatases consist of three general families with differentsubstrate specificities towards phospho-seryl/threonyl, -tyrosyl resi-dues, or both. This kit provides a simple means to measure phosphataseactivity by using pNPP as a general substrate of phosphatases. In a firststep, the enzyme dephosphorylates its substrate pNPP. In a second step,the phenolic OH-group is deprotonated under alkaline conditions result-ing in p-nitrophenolate with a strong absorption at 405 nm (yellow).


AK-101 Phosphatase Assay Kit 1 Kit(pNPP-based colorimetric assay,96 well format)


PR-348 Cdc25A 20 µg(Cell division cycle phosphatase) Human

PR-330 PRL-3 20 µg(Protein Tyrosine Phosphatase withC-terminal Prenylation Motif,Phosphatase of RegeneratingLiver)Human

PR-930 PTEN 5 µg(phosphatase and tensin homologuedeleted on chromosome 10) human

PR-931 PTP 1B 20 µg(Protein Tyrosine Phosphatase 1B) Human

PR-311 PTP-SL-cat 20 µg(Protein Tyrosine Phosphatase, CatalyticDomain) Mouse

PR-310 PTP-SL-ex 20 µg(Protein Tyrosine Phosphatase) Mouse

PR-319 PTP-SL-ex, catalytically inactive mutant 20 µg(Protein tyrosine phosphatase) mouse

PR-312 PTP-SL-kim 20 µg(Protein Tyrosine Phosphatase, KinaseInteraction Motif and Catalytic Domain)Mouse

PR-309 PTPµ, catalytic domain D1(N2) 20 µg(Protein tyrosine phosphatase) Human

PR-349 PTPµ, cytoplasmic region (D1D2) 20 µg(Protein tyrosine phosphatase) Human

PR-901 SHP-1GST 20 µg(Src homology 2 domain Phosphatase)Human

PR-350 VHR 20 µg(VH1-related phosphatase) Human

PR-308 PTP-BL, catalytic domain 20 µg(Protein tyrosine phosphatase Basophil-like)mouse


Assay Kits for Protein PhosphorylationSIGNAL TRANSDUCTIONKinase & Phosphatase Assay kits

� EnzoLyte™ Serine/Threonine Protein KinaseSubstrate Sampler Kit *FAM-Labeled*

The EnzoLyte™ Serine Protein Kinase Substrate Sampler Kit provides tenFAM-labeled protein kinase substrates along with two phosphorylatedpeptide controls. These substrates can be utilized to design fluorescencepolarization-based kinase assays, facilitating the identification of anappropriate peptide substrate for the serine kinase of interest.


71202 EnzoLyte™ Serine/Threonine Protein Kinase 1 kitSubstrate Sampler Kit *FAM-Labeled*

� EnzoLyte™ Tyrosine Protein Kinase SubstrateProfiling Kit *Fluorimetric*

EnzoLyte™ Tyrosine Protein Kinase (TPK) Substrate Sampler Kit is de-signed to facilitate the identification of the optimum peptide substratefor developing a particular TPK assay. The EnzoLyte™ Tyrosine ProteinKinase Substrate Profiling Kit provides four FAM-labeled protein kinasesubstrates, four biotin-labeled substrates, and two phosphorylated pep-tide controls.


71203 EnzoLyte™ Tyrosine Protein Kinase 1 kitSubstrate Profiling Kit *Fluorimetric*

The kit contains: • Four FAM-labeled TPK substrates • Four Biotin-labeledTPK substrates •Two phosphorylated peptide controls.

� EnzoLyte™ Protein Kinase Substrate Sampler Kit*Biotinylated*

EnzoLyte™ Tyrosine Protein Kinase (TPK) Substrate Sampler Kit*Biotinylated* is designed to facilitate the characterization of an appro-priate peptide substrate for developing a particular TPK assay. The kitcontains 10 popular biotinylated protein kinase substrates along with twophosphorylated peptide controls. It provides a convenient solution foridentifying a suitable substrate for designing biotinylated peptide-basedprotein kinase assays.


71200 EnzoLyte™ Protein Kinase Substrate 10 x 500Sampler Kit *Biotinylated* assays

� EnzoLyte™ Protein Kinase Substrate Sampler Kit*FAM-labeled*

EnzoLyte™ Tyrosine Protein Kinase (TPK) Substrate Sampler Kit *FAM-Labeled* is designed to facilitate the characterization of an appropriatepeptide substrate for developing a particular TPK assay. The kit con-tains 10 FAM-labeled protein kinase substrates along with two phospho-rylated peptide controls. It provides a convenient solution for identify-ing a suitable substrate for designing FAM-labeled peptide-based pro-tein kinase assays.


71201 EnzoLyte™ Protein Kinase 10 x 500Substrate Sampler Kit *FAM-labeled* assays

� EnzoLyte™ MFP Protein Phosphatase Assay Kit*Fluorimetric*

MFP is a proprietary fluorogenic substrate for most phosphatases, i.e.,alkaline phosphatases, acid phosphatases, protein tyrosine phosphatasesand serine/threonine phosphatases. The phosphatase-induced hydrolysisof MFP yields an intense fluorescent product that has excitation wave-length around 488 nm, and emission maximum around 520 nm. EnzoLyte™FMP Protein Phosphatase Assay Kit uses MFP to quantify protein phos-phatase activities. The kit can be used for characterizing kinetics ofenzyme reaction and high throughput screening of protein phosphataseinhibitors.


71104 EnzoLyte™ MFP Protein 500Phosphatase Assay Kit *Fluorimetric* assays

The kit contains: • MFP phosphatase substrate • Assay buffer

� EnzoLyte™ MG Phosphate Assay Kit*Colorimetric*

EnzoLyte™ MG Phosphate Assay Kit is based on quantification of thegreen complex formed between Malachite Green, molybdate and freeorthophosphate on a spectrophotometer (600-660 nm) or on a platereader. Assays can be performed in tubes, cuvettes or multi-well platesand can detect as little as 1.6 pmoles of phosphate (detection rangebetween 0.02 mM and 40 mM phosphate). The kit has been used forassaying phosphatase, phospholipase or lipase, nucleoside triphosphatase(ATPase, GTPase etc) and phytase.


71103 EnzoLyte™ MG Phosphate Assay Kit 1000*Colorimetric* assays

Contents:Highly purified Malachite Green reagent, Assay buffer

� EnzoLyte™ pNPP Protein Phosphatase Assay Kit*Colorimetric*

p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromoge-nic substrate for protein tyrosine phosphatases and serine/threoninephosphatases. The EnzoLyte™ pNPP Protein Phosphatase Assay Kit usespNPP to quantify protein phosphatase activities. The kit can be used forcharacterizing kinetics of enzyme reaction and high throughput screen-ing of protein phosphatase inhibitors. It has high sensitivity and widelinear range (The detection limit is generally 3 ng or below).


71105 EnzoLyte™ pNPP Protein 500Phosphatase Assay Kit *Colorimetric* assays

Contents: pNPP chromogenic substrate, Assay buffer

� Fluorescein diphosphate, tetraammonium salt(FDP)

Fluorescein diphosphate (FDP) is one of the most sensitive fluorogenicphosphatase substrates. The colorless and nonfluorescent FDP is hydro-lyzed to highly fluorescent fluorescein.


85300 Fluorescein diphosphate, 5 mgtetraammonium salt (FDP)

� Antibodies

We have a wide range of Antibodies for signaing related proteins. Pleaseenquire.


Antibodies and related productsANTIBODIES

Products

We offer more than 11,000 monoclonal and polyclonal antibodiesfor research and diagnosis and a vast number of secondary antibod-ies labeled to AP, Biotin, Colloidal Gold, Cyan, FITC, HRP, PE, TexasRed™ and TRITC are available. Also available are the antigens.

••••• Primary Antibodies

••••• Secondary Antibody Conjugates

••••• Epitope Tags, Fusion Proteins etc.

••••• Detection Kits

••••• Enzyme Substrates

••••• Avidin, Biotin, Streptavidin

••••• RIA-Kits

••••• Protein Standards & Controls

••••• Custom Antibody Production and Services

� ELISA Substrates

••••• OPD Fizzing Tablets

••••• TMB Fizzing Tablets

Other substrates also available

� WB/IHC Substrate

• AEC Fizzing Tablets

• FAST RED/ Naphtol

• Urea Peroxyde Tablets

• BCIP / INT

• p-NPP

• NBT/ ROCK

• TMB

� Immunoblotting and ImmunhistochemistrySubstates

••••• BCIP/ NBT

••••• BCIP/ TNBT

••••• DAB

••••• TMB Blotting

� Secondary Antibodies

••••• Alkaline Phosphatase

••••• Horse Radish Peroxidase

••••• Biotin (BAC) Conjugates

••••• Cyanine (CYTM) Conjugates

••••• DiI-AC-LDL / DiI-LDL Di0 –LDL

••••• F (ab’) Fragments and F (ab’) 2 Fragments of Antibodies

••••• Gold Labelled Secondary Antibodies and F (ab’) 2 Fragments

••••• Fluorescein Conjugates (FITC)

••••• Peroxide Anti-Peroxidase (PAP)

••••• Phycocyanin and Allophycocyanin

••••• Phycoerythrin Conjugates

••••• Rhodamine and Texas RedTM Conjugates

� Secondary Antibodies


28164 Rabbit Anti-Mouse IgG (H+L), purified 1 mg

28165 Rabbit Anti-Mouse IgG (H+L), HRP-Conjugated 0.5 mg

28166 Rabbit Anti-Mouse IgG (H+L), AP-Conjugated 0.5 mg

28167 Rabbit Anti-Mouse IgG (H+L), Biotinylated 0.5 mg

28168 Rabbit Anti-Goat IgG (H+L), purified 1 mg

28169 Rabbit Anti-Goat IgG (H+L), HRP-Conjugated 0.5 mg

28170 Rabbit Anti-Goat IgG (H+L), AP-Conjugated 0.5 mg

28171 Rabbit Anti-Goat IgG (H+L), Biotinylated 0.5 mg

28172 Goat Anti-Mouse IgG (H+L), purified 1 mg

28173 Goat Anti-Mouse IgG (H+L), HRP-Conjugated 0.5 mg

28174 Goat Anti-Mouse IgG (H+L), AP-Conjugated 0.5 mg

28175 Goat Anti-Mouse IgG (H+L), Biotinylated 0.5 mg

28176 Goat Anti-Rabbit IgG (H+L), purified 1 mg

28177 Goat Anti-Rabbit IgG (H+L), HRP-Conjugated 0.5 mg

28178 Goat Anti-Rabbit IgG (H+L), AP-Conjugated 0.5 mg

28179 Goat Anti-Rabbit IgG (H+L), Biotinylated 0.5 mg

29709 Rabbit Anti-Chicken IgY, purified 1 mg

29710 Rabbit Anti-Chicken IgY, HRP-Conjugated 0.5 mg

29711 Rabbit Anti-Chicken IgY, AP-Conjugated 0.5 mg

29712 Rabbit anti-Chicken IgY, Biotinylated 0.5 mg


29774 Anti-C-Myc Tag 100 µg

29779 Anti-GFP 100 µg

29674 Anti-DYKDDDDK 100 µg

29629 Anti-HA Tag 100 µg

29673 Anti-His Tag, rabbit polyclonal 100 ug

61250 Anti-His Tag IgG, mouse monoclonal 100 ug

29673-BIOT Rabbit Polyclonal Anti-His Tag IgG, 100 µgBiotinylated

29673-FITC Rabbit Polyclonal Anti-His Tag IgG, FITC- 100 µglabeled, Also available in other Fluorescenttags like FAM, AMCA, HiLyte Fluor™ ,TAMRA

61250-FITC Mouse Monoclonal Anti-His Tag IgG, FITC- 100 µglabeled, Also available in other Fluorescenttags like FAM, AMCA, HiLyte Fluor™ ,TAMRA

61250-BIOT Mouse Monoclonal Anti-His Tag IgG, 100 µgBiotinylated

29531 Monoclonal Anti-GST 100 µg

29531-BIOT Anti-GST Tag, Mouse Monoclonal, Biotinylated 100 ug

29531-FITC Anti-GST Tag, Mouse Monoclonal, FITC labeled; 100 µgAlso available in other Fluorescent tagslike FAM, TAMRA, AMCA, HiLyte Fluor™

� Epitope Tag antibodies


Biotin Derivatives, Avidin Conjugates

� AnaTag™ Protein Biotinylation Kit

It includes all essential components for biotinylation and purificationwith a detailed operation protocol.


71003 AnaTag™ Protein Biotinylation Kit 1 kit

� Amino-Reactive Biotins


60638 Biocytin, [e-Biotinoyl-L-lysine] 100 mgMore polar than biotin; used for cell tracing

21100 D-Biotin 1 gHighly purified and tested for avidin-binding

60639 D-Biotin *100 mM aqueous solution* 10 mlReady-to-use solution

21109 D-Biotin, SE [Succinimidyl D-biotin] 100 mgPopular biotinylating reagent fornucleotides, peptides and proteins

21113 Biotin-X, SE [6-((Biotinoyl)amino) 100 mghexanoic acid, succinimidyl ester]Amino-reactive biotinylating reagentfor peptides and proteins;

21115 Biotin-X, SSE [6-((Biotinoyl)amino) 100 mghexanoic acid, sulfosuccinimidyl ester,sodium salt]

60640 Biotin-XX, SE [6-((6-((Biotinoyl)amino) 25 mghexanoyl)amino) hexanoic acid,succinimidyl ester]Biotinylating reagent for peptides andproteins

21117 FMOC biocytin [FMOC-Lys(Biotin)-OH] 100 mgBuilding block for preparingbiotinylated peptides

� Amino-containing Biotins for ModifyingCarbohydrates, Carbonyl, Carboxyl Groups


60647 Biocytin hydrazide 25 mgMore polar than biotin; used for cell tracing

60648 Biotin cadaverine [N-(5-Aminopentyl) 25 mgbiotinamide, trifluoroacetic acid salt]Building block for modifying carboxyand carbonyl groups; substratefor transglutaminase

60649 Biotin ethylenediamine [N-(2-Aminoethyl) 25 mgbiotinamide, hydrobromide]Building block for modifying carboxy andcarbonyl groups

60650 Biotin hydrazide 25 mgBiotinylating reagent for carbohydratesand carbonyl compounds

60651 Biotin-X cadaverine [5-(((N-(Biotinoyl)amino) 25 mghexanoyl)amino) pentylamine,trifluoroacetic acid salt]Building block for modifying carboxy andcarbonyl groups; substrate fortransglutaminase; longer spacer than 60648

60652 Biotin-XX hydrazide [6-((6 25 mg((Biotinoyl)amino)hexanoyl)amino)hexanoic acid, hydrazide]Biotinylating reagent for carbohydratesand carbonyl compounds; betteravidin-binding due to the longer spacer

21117 FMOC biocytin [e-Biotinoyl-a-(9 100 mgfluorenylmethoxycarbonyl)-L-lysine]Building block for preparingbiotinylated peptides

ANTIBODIESBiotin-Avidin

� Amino-containing Biotins for ModifyingCarbohydrates, Carbonyl, Carboxyl Groups


60645 ARP [N-(aminooxyacetyl)-N’-(D-biotinoyl) 10 mghydrazine, trifluoroacetic acid salt]Biotinylating reagent for carbohydratesand nucleic acids

60638 Biocytin [e-Biotinoyl-L-lysine] 100 mgMore polar than biotin; used for cell tracing

� Thiol-Reactive Biotins


60642 Biocytin C2 maleimide 10 mgExcellent thiol-reactive biotin with amoderate spacer for better avidin-binding

60643 Biotin C2 maleimide 25 mgExcellent thiol-reactive biotin with amoderate spacer for better avidin-binding

60644 N-(Biotinoyl)-N’-(iodoacetyl)ethylenediamine 25 mgThiol-reactive biotinylating reagentfor proteins and other biomolecules.



60659 Streptavidin 5 mgHighly purified and tested for biotin-binding

60660 Streptavidin, alkaline phosphatase 0.25 mlconjugated *2 mg/mL*used in ELISA assays

60661 Streptavidin, allophycocyanin 0.10 mlconjugated *2 mg/mL*λ

abs: 652 nm; λ

em: 662 nm

used in immunofluorescence assays

60662 Streptavidin, B-phycoerythrin 0.25 mlconjugated *2 mg/mL*λ

abs: 545 nm; λ

em: 575 nm


60663 Streptavidin, crosslinked allophycocyanin 0.10 mlconjugated *2 mg/mL*λ

abs: 616 nm; λ

em: 647 nm


60664 Streptavidin, 5-FAM conjugated 1 mgλ

abs: 492 nm; λ

em: 519 nm


60665 Streptavidin, HiLyte Fluor™ 488 conjugated 1 mgλ

abs: 495 nm; λ

em: 524 nm

Widely used in immunofluorescence assays;stronger signal and better stabilitythan FITC-streptavidin


abs: 555 nm; λ

em: 565 nm

Widely used in immunofluorescence assays;comparable spectral propertiesto those of Cy3 or Cy3B


abs: 650 nm; λ

em: 668 nm

Widely used in immunofluorescence assays;comparable spectral propertiesto those of Cy5

60668 Streptavidin, HRP conjugated 1 mgλ

abs: N/A nm; λ

em: N/A nm

Widely used in ELISA assays

60669 Streptavidin, R-phycoerythrin 0.25 mlconjugated *2 mg/mL*λ

abs: 565 nm; λ

em: 575 nm

Widely used in immunofluorescence assays

60670 Streptavidin, 5-TAMRA conjugated 1 mgλ

abs: 541 nm; λ

em: 568 nm


60671 Streptavidin, HiLyte Fluor™ TR conjugated 1 mgλ

abs: 591 nm; λ

em: 622 nm

used in immunofluorescence assays;comparable spectral properties tothose of sulforhodamine 101 sulfonylchloride (also called Texas Red® )with stronger fluorescence intensity

Biotin, Avidin, StreptavidinANTIBODIES

..

ANTIBODIESBiotin-Avidin

� Streptavidin and Its Conjugates � Biotin Quantitation Reagents and FluorescentBiotins


71161 HABA Biotin Quantitation Kit *Colorimetric* 1 kitλ

abs: 348 nm; λ

em: N/A nm

Colorimetric determination of biotin

� Antibody Reagents


29704 Antibody Diluent (Ready to Use) 125 mL

29702 AnaPrep™ IHC Histain Kit-AEC 15 mL

29703 AnaPrep™ AEC Substrate System 125 mL

29700 AnaPrep™ IHC Histain Kit-DAB 15 mL

29551 Maleimidyl KLH 5 mg

29552 Maleimidyl BSA 5 mg


60654 Biotin-4-fluorescein 10 mgλ

abs: 494 nm; λ

em: 523 nm

Demonstrate better binding andstronger fluorescence than biotinfluorescein

60655 Biotin-X NTA [Biotin-X nitrilotriacetic acid, 5 mgtripotassium salt]Excellent reagent for detectingpolyhistidine-containing biomolecules

60656 Fluorescein biotin [5-((N-(5-(N-(6 5 mg(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein]λ

abs: 494 nm; λ

em: 518 nm

Green fluorescent biotin used forstudying avidin binding

60657 HABA [2-4’(-hydroxyazobenzene) 1 gbenzoic acid] *UltraPure Grade*λ

abs: 348 nm; λ

em: N/A nm

Colorimetric indicator for biotin

60658 TMR Biocytin [5-(and-6)- 5 mgTetramethylrhodaminebiocytin] λ

abs: 554 nm; λ

em: 581 nm

Orange fluorescent biotin used forstudying avidin binding



PR-902 PrPc (full length, residues 23-231) His 50 µg(Prion Protein, cellular form) Human,Recombinant, E. coli

PR-908 Native PrPc (full length, residues 5 µg23-231) His (Prion Protein, cellular form)Human, Recombinant, E. coli

PR-905 PrPc (globular domain, residues 50 µg90-231) His (Prion Protein, cellularform)Human, Recombinant, E. coli

PR-911 Native PrPc (globular domain, residues 5 µg90-231) His (Prion Protein, cellularform)Human, Recombinant, E. coli


PR-370 PrPc (wild type)His 50 µg(Prion Protein, cellular form)Bovine, Recombinant, E. coli

PR-3v71 PrPc (T194A)His 50 µg(Prion Protein, cellular form)Bovine, Recombinant, E. coli

PR-372 PrPc (F209S)His 50 µg(Prion Protein, cellular form)Bovine, Recombinant, E. coli

PR-373 PrPc (Q228R)His 50 µg(Prion Protein, cellular form)Bovine, Recombinant, E. coli

PR-374 PrPc (A144V)His 50 µg(Prion Protein, cellular form)Bovine, Recombinant, E. coli


PR-376 PrPcHis Genotyp: ARR (Codons 136,154,171), 50 µgscrapie resistant ovine (sheep),Recombinant, E. coli

PR-377 PrPcHis Genotyp: VRQ 50 µg(Codons 136,154,171), scrapie sensitiveovine (sheep), Recombinant, E. coli


PR-903 PrPc (full length, residues 23-231)His 50 µg(Prion Protein, cellular form)Hamster, Recombinant, E. coli

PR-909 Native PrPc (full length, 50 µgresidues 23-231)His

(Prion Protein, cellular form)Hamster, Recombinant, E. coli

PR-906 PrPc (globular domain, residues 50 µg90-231)His


PR-912 Native PrPc (globular domain, 50 µgresidues 90-231)His


Prion Proteins and cDNAsRECOMBINANT PROTEINS

Prion Proteins

� Mouse Prion Proteins


PR-904 PrPc (full length, residues 23-230)His 50 µg(Prion Protein, cellular form)Mouse, Recombinant, E. coli

PR-910 Native PrPc (full length, residues 23-230)His 5 µg(Prion Protein, cellular form)Mouse, Recombinant, E. coli

PR-907 PrPc (globular domain, residues 90-230)His 50 µg(Prion Protein, cellular form)Mouse, Recombinant, E. coli

PR-913 Native PrPc (globular domain, 5 µgresidues 90-230)His

(Prion Protein, cellular form)Mouse, Recombinant, E. coli

� Prion associated Proteins

N-terminal GST tagged Src SH3 domain. Src SH3 domain is the SH3 do-main of Src tyrosine kinase. SH3 domains are known to bind proline richpeptides.

Human lymphocyte specific kinase (Lck) is involved in T-cell- and IL2-receptor signaling. Lck SH3 domain is the SH3 domain of Lck tyrosinekinase. SH3 domains are known to bind proline rich peptides.

GABARAP is essential for correct delivery of newly synthesized or re-cycled GABA type A receptor molecules to the postsynaptic membraneneurons.


PR-338 SrcGST SH3 domain(Sarcoma 50 µginducing tyrosine kinase,SH3 domain) Human

PR-339 LckGST SH3 domain(Lymphocyte- 50 µgspecific Kinase, SH3 Domain)Human,

PR-1000 GABARAPGST(GABA Type 50 µgA Associated Protein)Human,


PRD-370 bovPrPc cDNA, wild type(cDNA from bovine Prion Protein,cellular form) 10 µg

PRD-371 bovPrPc cDNA [T194A](cDNA from bovine Prion Protein,cellular form) 10 µg

PRD-372 bovPrPc cDNA [F209S](cDNA from bovine Prion Protein,cellular form) 10 µg

PRD-373 bovPrPc cDNA [Q228R](cDNA frombovine Prion Protein, cellular form) 10 µg

PRD-374 bovPrPc cDNA [A144V](cDNA frombovine Prion Protein, cellular form) 10 µg

� HumanPrion Proteins

� Bovine Prion Proteins

� Ovine (Sheep) Prion Proteins

� Hamster Prion Proteins

� BovPrPC cDNA

ATG-start lodon and different restriction sites for subcloning on request.


Viral Proteins- CMV, EBV, Herpes, Hepatitis A, B, CRECOMBINANT PROTEINS

Viral Proteins

� Cytomegalovirus (CMV)


PR-1247 CMV-gB (residues 11-76) 100 µg(Cytomegalo Virus Glycoprotein B)

PR-1248 CMV pp28 (residues 130-160) 100 µg(Cytomegalo Virus Phosphoprotein 28)


PR-1250 CMV pp52 (residues 202-434) 100 µg(Cxtomegalo Virus Phosphoprotein 52)



� Epstein-Barr Virus (EBV)


PR-1223 EBV-EBNA1 Mosaic 100 µg(residues 1-90, 408-498)(Epstein-Barr Virus Nuclear Protein 1)

PR-1224 EBV-EA (residues 306-390) 100 µg(Epstein-Barr Virus Early Antigen)

PR-1225 EBV p18 (residues 1-119) 100 µg(Epstein-Barr Virus Capsid Antigen)

PR-1226 EBV p23 (residues 1-162) 100 µg(Epstein-Barr Virus Capsid Antigen)


PR-1108 HSV-1 gD (residues 266-394) 100 µg(Herpes Simplex Virus-1 glycoprotein D)

PR-1109 HSV-1 gG (residues 84-175) 100 µg(Herpes Simplex Virus-1 glycoprotein G)

PR-1110 HSV-2 gD (residues 266-384) 100 µg(Herpes Simplex Virus-2 glycoprotein D)

PR-1111 HSV-2 gG (residues 525-578) 100 µg(Herpes Simplex Virus-2 glycoprotein G)

� Herpes


PR-1122 HBV-Core (residues 1-186) 100 µg(Hepatitis B Virus Core Protein)

PR-1124 HBV-Core 16 kDa (residues 1-144) 100 µg(Hepatitis B Virus Core Protein)

PR-1123 HBV-Core 18 kDa (residues 1-183) 100 µg(Hepatitis B Virus Core Protein)

PR-1125 HBV-HBe 100 µg(Hepatitis B Virus e Antigen, HBVeAg)

PR-1126 HBVsAg-ayw 100 µg(Hepatitis B Virus Surface Antigen,ayw subtype)

PR-1129 HBVsAg-ayw 100 µg(Hepatitis B Virus Surface Antigen,ayw subtype)

PR-1127 HBVsAg-adr 100 µg(Hepatitis B Virus Surface Antigen,adr subtype)

PR-1128 HBVsAg-adw 100 µg(Hepatitis B Virus Surface Antigen,adw subtype)

� Hepatitis B


PR-1114 HAV-VP1-P1A/Mosaic 100 µg(residues 669-782, 722-830)(Hepatitis A Virus Coat ProteinVP1-Core Protein P2A Mosaic)

PR-1113 HAV-VP1-P2A (residues 669-782) 100 µg(Hepatitis A Virus Coat ProteinVP1- Core Protein P2A)

PR-1117 HAV-P2C-P3A/1 (residues 1392-1521) 100 µg(Hepatitis A Virus Core Protein P2C-P3Afragment 13-15)

PR-1118 HAV-P2C-P3A/2 (residues 1492-1606) 100 µg(Hepatitis A Virus Core Protein P2C-P3Afragment 14-16)

PR-1116 HAV-P2C-P3A/Mosaic 100 µg(residues 1392-1521, 1492-1606)(Hepatitis A Virus Core ProteinsP2C-P3A Mosaic)

PR-1115 HAV-P2C (residues 1211-1234) 100 µg(Hepatitis A Virus Core Protein P2C)

PR-1121 HAV-P3C (residues 1643-1743) 100 µg(Hepatitis A Virus Core Protein P3C)

� Hepatitis A (Contd.)

� Hepatitis A


PR-1112 HAV-VP1 (residues 524-633) 100 µg(Hepatitis A Virus Coat Protein VP1)

PR-1120 HAV-VP2-VP4 (residues 55-164) 100 µg(Hepatitis A Virus Capsid ProteinsVP4-VP2)

PR-1119 HAV-VP3 (residues 304-415) 100 µg(Hepatitis A Virus VP3 Capsid Protein)

� Hepatitis C


PR-1131 HCV-N-G1 (residues 1-102) 100 µg(Hepatitis C Virus Nucleocapsid Genotype 1)

PR-1130 HCV-N-G1a (residues 2-119) 100 µg(Hepatitis C Virus Nucleocapsid Genotype 1a)

PR-1132 HCV-N-G1b (residues 2-119) 100 µg(Hepatitis C Virus Nucleocapsid Genotype 1b)










PR-1140 HCV-N-G3/10 (residues 2-119) 100 µg(Hepatitis C Virus Nucleocapsid Genotype 3/10)

PR-1141 HCV-N (residues 105-302) 100 µg(Hepatitis C Virus Nucleocapsid)

PR-1142 HCV-N24 100 µg(Hepatitis C Virus Nucleocapsid)

PR-1143 C-HCV 100 µg(Combined Hepatitis C Virus, NS3/NS4/NS5)

PR-1144 HCV-NC 22 kDa (residues 2-192) 100 µg(Hepatitis C Virus Nucleocapsid)

PR-1144-B HCV-NC 22 kDa (residues 2-192), 100 µgHRP Biotin conjugated

(Hepatitis C Virus Nucleocapsid)

PR-1144-F HCV-NC 22 kDa (residues 2-192), 100 µgFluorescein conjugated(Hepatitis C Virus Nucleocapsid)

PR-1144-R HCV-NC 22 kDa (residues 2-192), 100 µgRhodamine conjugated(Hepatitis C Virus Nucleocapsid)

PR-1145 HCV-NS3His 100 µg(Hepatitis C Virus Non-Structural protein)

PR-1146 HCV-NS3-S1a (residues 1192-1459) 100 µg(Hepatitis C Virus Non-Structural protein,Subtype 1a)

PR-1153 HCV-NS3-S1a (residues 1359-1456) 100 µg(Hepatitis C Virus Non-Structural protein,Subtype 1a)

PR-1147 HCV-NS3-S1b (residues 1192-1459) 100 µg(HCV Non-Structuralprotein, Subtype 1b)

PR-1154 HCV-NS3-S1b (residues 1359-1456) 100 µg(HCV Non-Structural protein,Subtype 1b)

PR-1148 HCV-NS3-S1c (residues 1192-1459) 100 µg(HCV Non-Structural protein,Subtype 1c)


Viral Proteins- Hepatitis CRECOMBINANT PROTEINS

Viral Proteins

� Hepatitis C (Contd.) � Hepatitis C (Contd.)



PR-1149 CV-NS3-S2c (residues 1192-1459) 100 µg(HCV Non-Structural protein,Subtype 2c)

PR-1156 HCV-NS3-S3 (residues 1359-1456) 100 µg(HCV Non-Structural protein,Subtype 3)



PR-1152 HCV-NS3-S5a (residues 1192-1459) 100 µg(HCV Non-Structural protein,Subtype 5a)


PR-1151 HCV-NS3-S6a (residues 1192-1459) 100 µg(HCV Non-Structural protein,Subtype 6a)

PR-1160 HCV-NS3-22 kDa (residues 1400-1643) 100 µg(HCV Non-Structural protein)

PR-1160-B/ HCV-NS3 22 kDa (residues 1400-1643), 100 µgHRP Biotin conjugated

(HCV Non-Structural protein)

PR-1161 HCV-NS4, Mosaic 100 µg(HCV Non-Structural protein)

PR-1162 HCV-NS4-G1, Mosaic (residues 1691- 100 µg1710, 1712-1733, 1921-1940)(HCV Non-Structural protein, Genotype 1)

PR-1163 HCV-NS4-G2, Mosaic (residues 100 µg1691-1710, 1712-1733, 1921-1940)(HCV Non-Structural protein, Genotype 2)

PR-1164 HCV-NS4-G3, Mosaic (residues 1691- 100 µg1710, 1712-1733, 1921-1940)(HCV Non-Structural protein, Genotype 3)

PR-1165 HCV-NS4-G5, Mosaic (residues 100 µg1691-1710, 1712-1733, 1921-1940)(HCV Non-Structural protein, Genotype 5)

PR-1166 HCV-NS4 (residues 1916-1947) 100 µg(HCV Non-Structural protein)

PR-1167 HCV-NS4 19kDa (residues 1658-1863) 100 µg(HCV Non-Structural protein)

PR-1167-B HCV-NS4 19kDa (residues 1658-1863), 100 µgBiotin conjugated(HCV Non-Structural protein)

PR-1167- HCV-NS4 19kDa (residues 1658-1863), 100 µgHRP Horseradish peroxidase conjugated

(HCV Non-Structural protein)

PR-1167-F HCV-NS4 19kDa (residues 1658-1863), 100 µgFluorescein conjugated(HCV Non-Structural protein)


Viral Proteins- Hepatitis C, HIV-1RECOMBINANT PROTEINS

Viral Proteins

� Hepatitis C (Contd.)


PR-1167-R HCV-NS4 19kDa (residues 1658-1863), 100 µgRhodamine conjugated(HCV Non-Structural protein)

PR-1167-R HCV-NS4 19kDa (residues 1658-1863), 100 µgRhodamine conjugated(HCV Non-Structural protein)

PR-1168 HCV-NS5 (residues 2322-2423) 100 µg(HCV Non-Structural protein)

PR-1169 HCV-NS5-G1 100 µg(HCV Non-Structural protein, Genotype 1)

PR-1170 HCV-NS5-G1a (residues 2212-2313) 100 µg(HCV Non-Structural protein, Genotype 1a)

PR-1171 HCV-NS5-G1b (residues 2212-2313) 100 µg(HCV Non-Structural protein, Genotype 1b)





PR-1176 HCV-NS5-G3a (residues 2212-2313) 100 µg(HCV Non-Structural protein,Genotype 3a)






� Hepatitis D

� Hepatitis E


PR-1182 HDV (residues 1-108, 151-209)(Hepatitis Delta Virus) 100 µg


PR-1183 HEV-ORF2/ORF3/1 Mosaic(Hepatitis E Virus ORFproteins, 4 immunodominant regions) 100 µg

PR-1184 HEV-ORF2/ORF3/2 Mosaic(Hepatitis E Virus ORFproteins, 12 immunodominant regions) 100 µg

PR-1187 HEV-ORF2 (residues 92-123)(Hepatitis E Virus ORF protein) 100 µg

� Hepatitis E (Contd.)


PR-1186 HEV-ORF2 (residues 403-461) 100 µg(Hepatitis E Virus ORF Frame protein)

PR-1188 HEV-ORF2 (residues 452-617) 100 µg(Hepatitis E Virus ORF protein)

PR-1185 HEV-ORF2 (residues 633-659) 100 µg(Hepatitis E Virus ORF protein)


PR-1200 HIV-1 Envelope (HIV 1 Antigen) 100 µg

PR-1201 HIV-1 p24 (HIV 1 Antigen) 100 µg

PR-1201-B HIV-1 p24, biotin conjugated 100 µg(HIV 1 Antigen)

PR-1201- HIV-1 p24, horse radish peroxidase labeled 100 µgHRP (HIV 1 Antigen)

PR-1202 HIV-1 p17, p24, gp120, gp41 100 µg(HIV 1 Antigens)

PR-1203 HIV-1 p17, p24, gp120 (HIV 1 Antigens) 100 µg

PR-1207 HIV-1 p17, p24 (HIV 1 Antigens) 100 µg

PR-1213 HIV-1 p24 (residues 77-436) (HIV 1 Antigen) 100 µg

PR-1204 HIV-1 gp41 (HIV 1 Antigen) 100 µg

PR-1204-B HIV-1 gp41, biotin conjugated(HIV 1 Antigen) 100 µg

PR-1204- HIV-1 gp41, horse radish peroxidaseHRP conjugated (HIV 1 Antigen) 100 µg

PR-1205 HIV-1 gp41 Long (HIV 1 Antigen) 100 µg

PR-1205- HIV-1 gp41 Long, horse radishHRP peroxidase conjugated

(HIV 1 Antigen) 100 µg

PR-1206 HIV-1 gp41 (residues 466-753)(HIV 1 Antigen) 100 µg

PR-1217 HIV-1 p55 (HIV 1 Antigen) 10 µg

PR-1215 HIV-1 gp160 (HIV 1 Antigen) 10 µg

PR-1209 HIV-type O Envelope 100 µg(HIV Type O Antigen)

PR-1208 HIV-1 Integrase (HIV 1 Integrase) 100 µg

PR-1216 HIV-1 TAT 10 µg(HIV 1 Trans-Acting Transcription factor)

PR-381 HIV-1 Nef protein (full length, 1-210)His 50 µg((HIV -1, Negative Factor)

PR-382 HIV-1 Nef protein (full length, 2-210)His, 50 µgmyristoylated(HIV-1, Negative Factor)

PR-1214 HIV-1 Nef (residues 3-190) 100 µg(HIV 1 Antigen)

PR-351 HIV-1 RT 10 µg(HIV 1 Reverse Transcriptase)

PR-353 HIV-1 RT, p51 subunit 10 µg(HIV-1 Reverse Transcriptase)

PR-354 HIV-1 RT, p66 subunit 10 µg(HIV 1 Reverse Transcriptase)

PR-380 HIV-1 Vpu-43GST 100 µg(HIV 1, Virus Protein U, residues 39-81

� HIV-1


HIV-2, Rubella, VZV, TBEV, Taxoplasma, TreponemaRECOMBINANT PROTEINS

Viral Proteins

� HIV-2


PR-1218 HIV-2 Envelope(HIV 2 Antigen) 100 µg

PR-1210 HIV-2 gp32 100 µg(HIV 2 Antigen)

PR-1211 HIV-2 gp36 100 µg(HIV 2 Antigen)

PR-1212 HIV-2 gp36 (residues 390-702) 100 µg(HIV 2 Antigen)

PR-352 HIV-2 RT 10 µg(HIV 2 Reverse Transcriptase)


PR-1100 SARS-ACE (residues 1-76) 100 µg(SARS-Associated Coronavirus Envelope)

PR-1101 SARS-ACM (residues 182-216) 100 µg(SARS-Associated Coronavirus Matrix)

PR-1103 SARS-ACN/2 (residues 1-49) 100 µg(SARS-Associated Coronavirus Nucleocapsid)

PR-1102 SARS-ACN/1 (residues 340-390) 100 µg(SARS-Associated Coronavirus Nucleocapsid)

PR-1104 SARS-ACN/3 (residues 1-49, 192-220) 100 µg(SARS-Associated Coronavirus Nucleocapsid)

PR-1105 SARS-ACSMS(C) (residues 1051-1076, 100 µg1121-1154, 1162-1190)(SARS-Associated Coronavirus Spike Mosaic S(C))

PR-1106 SARS-ACSMS(M) (residues 408-470, 540-573) 100 µg(SARS-Associated Coronavirus Spike Mosaic S(M))

� SARS


PR-1253 VZV-gE (aa 48-135) 100 µg(Varicella-zoster Virus Glycoprotein E)

PR-1254 VZV ORF9 (residues 6-28, 76-100) 100 µg(Varicella-zoster Virus Protein)

PR-1255 VZV ORF26 (residues 9-33, 184-208) 100 µg(Varicella-zoster Virus Protein)

� Varizella-Zoster-Virus (VZV)

� Rubella Virus (RV)


PR-1228 RV gE1 Mosaic (residues 100 µg157-176, 374-390, 213-239)(Rubella Virus Glycoprotein E1)

PR-1229 RV gE2 (residues 31-105) 100 µg(Rubella Virus Envelope Glycoprotein E2)

PR-1230 RV-C (residues 1-123) 100 µg(Rubella Virus Capsid Protein)


PR-1219 HTLV-1 Envelope 100 µg

PR-1220 HTLV-1 p24 Core 100 µg

PR-1221 HTLV-1 Mosaic (residues 374-400, 100 µg190-207)

PR-1222 HTLV-1+2 (fusion protein) 100 µg

� Human T-cell Lymphotropic Virus (HTLV)


PR-1227 TBEV gE (residues 95-229) 100 µg(Tick-borne Encephalitis VirusGlycoprotein E)


PR-1243 TG MIC3 (residues 234-306) 100 µg(Toxoplasma Gondii Microneme Protein 3)

PR-1244 TG GRA1/p24 (residues 57-149) 100 µg(Toxoplasma Gondii Dense Granule Protein 1)

PR-1245 TG GRA7/p29 (residues 24-100) 100 µg(Toxoplasma Gondii Dense Granule Protein 7)

PR-1246 TG SAG1/p30 (residues 45-198) 100 µg(Toxoplasma Gondii Surface Antigen 30)


PR-1239 TRP p15 100 µg(T. Pallidum Immunogen)


PR-1240 TRP p41 100 µg(T.Pallidum Immunogen)


PR-1242 TmpA 100 µg(T.Pallidum Protein A)

Tick-Borne Encephalitis Virus (TBEV)

Toxoplasma

Treponema


Transcription Factors - General, ActivatorsRECOMBINANT PROTEINS



PR-703 TBP 10 µg(TATA box Binding Protein)

PR-781 TBPGST 10 µg(TATA box Binding Protein)

PR-701 TFIIA-p12 10 µg(Transcription Factor IIA, p12 subunit)

PR-786 TFIIAGST (p12) 10 µg(Transcription Factor IIA, p12 subunit)

PR-785 TFIIAGST (p19) 10 µg(Transcription Factor IIA, β-subunit)

PR-700 TFIIA-p55 10 µg(Transcription Factor IIA, p55 subunit)

PR-784 TFIIAGST (p55)(Transcription Factor IIA, p55 subunit) 10 µg

PR-731 TFIIA 10 µg(Transription Factor IIA, reconstituted)

PR-702 TFIIB (Transcription Factor IIB) 10 µg

PR-787 TFIIBGST (Transcription Factor IIB) 10 µg

PR-712 TFIID (Transcription Factor IID, 2 µgnative complex)

PR-706 TFIIE 10 µg(Transcription Factor IIE, α- + β-subunits)

PR-704 TFIIEααααα, p56 10 µg(Transcription Factor IIE, α-subunit)

PR-788 TFIIEαααααGST, p56 10 µg(Transcription Factor IIE, α-subunit)

PR-705 TFIIEβββββ, p34 10 µg(Transcription Factor IIE, β-subunit)

PR-709 TFIIF 10 µg(Transcription Factor IIF, Rap30 +Rap74 subunits)

PR-707 TFIIF, RAP74 10 µg(Transcription Factor IIF, Rap74 subunit)

PR-789 RAP74GST 10 µg(Transcription Factor IIF, Rap74 subunit)

PR-708 TFIIF, RAP30 10 µg(Transcription Factor IIF, Rap30 subunit)

PR-790 RAP30GST 10 µg(Transcription Factor IIF, Rap30 subunit)

PR-710 TFIIH 2 µg(Transcription Factor IIH, native complex)

PR-711 TFIIH-p62 10 µg(Transcription Factor IIH, p62 subunit)

PR-713 RNA pol II 2 µg(RNA Polymerase II, native complex)

PR-714 RNA pol II-CTD 10 µg(RNA Polymerase II,Carboxy-terminal Domain)

PR-796 RNA pol II-CTDGST 10 µg(RNA Polymerase II, Carboxy-terminal Domain)

PR-715 RNA pol II-hRPB5 10 µg(RNA Polymerase II, p33 subunit)

PR-791 RNA pol II-hRPB5GST 10 µg(RNA Polymerase II, p33 subunit)

PR-792 RNA pol II-hRPB6GST 10 µg(RNA Polymerase II, p15.6 subunit)

� Transcription Factors – Activators

� General Transcription Factors (Contd.)� General Transcription Factors


PR-716 RNA pol II-hRPB9(RNA Polymerase II, p14.5 subunit) 10 µg

PR-793 RNA pol II-hRPB9GST

(RNA Polymerase II, p14.5 subunit) 10 µg


(RNA Polymerase II, RPB10 subunit) 10 µg


(RNA Polymerase II, RPB12 subunit) 10 µg

PR-769 Bcl-10 6 µg(B-cell lymphoma 10)

PR-763 BRCA1 2 µg(Breast/Ovary Cancer Gene 1 Product,Tumor Suppressor Protein andTranscription Factor)

PR-734 CTF1 5 µg(CCAAT-Box-Binding Transcription Factor 1)

PR-798 CTF1GST-Tag 0 µg(CCAAT-Box-Binding Transcription Factor1, activation domain, residues 399-499)

PR-771 c-fos 5 µg(Proto-oncogene, Cellular oncogene fos,G0/G1 switch regulatory protein 7)

PR-772 c-jun 5 µg(Proto-oncogene, TranscriptionFactor Activator Protein-1)

PR-773 c-myc 5 µg(Proto-oncogene)

PR-730 E2F-1 5 µg(RBAP-1, Transcription Factor andRb-mediator)

PR-809 FGF-1 5 µg(Acidic Fibroblast Growth Factor)

PR-810 FGF-2 5 µg(Basic Fibroblast Growth Factor)

PR-717 GAL4-AH 10 µg[Positive regulator of galactose induciblegenes, GAL4(1-147) fused to an a-Helix]

PR-718 GAL4-VP16 10 µg[Positive regulator of galactose induciblegenes, GAL4(1-147) fused toVP16(411-490) herpes simplex virus viriontransactivating protein]

PR-797 VP16GST 10 µg[VP16(411-490), herpes simplexvirus virion transactivating protein]i

PR-719 GAL4-E1A 4 µg[Positive regulator of galactose induciblegenes, GAL4(1-147) fused to E1A(121-223),Ubiquitin-like 1 activating enzyme E1A(SUMO-1 activating enzyme]



Transcription Factors - Activators, Coactivators, RepressorsRECOMBINANT PROTEINS


PR-720 GAL4-Sp1Q 4 µg[Positive regulator of galactose induciblegenes, GAL4(94) fused to transcriptionfactor Sp1(266-516)]

PR-732 Sp1 (HeLa) 2 µg(GC-box Binding Protein, Natural fraction)

PR-733 Sp1 (Sf9) 5 µg(GC-box Binding Protein )

PR-799 Sp1GST 10 µg(GC-box Binding Protein, GST-tagged)

PR-759 p53 5 µg(Tumor Suppressor Protein andTranscription Factor, wild type)

PR-760 p53 (C-terminal deletion 1-363) 5 µg(Tumor Suppressor Protein and TranscriptionFactor, residues 1-363, C-terminal deletion)

PR-761 p53 (C-terminal deletion 1-342) 5 µg(Tumor Suppressor Protein and TranscriptionFactor, residues 1-342, C-terminal deletion)

PR-782 p53GST (wild type) 5 µg(Tumor Suppressor Protein andTranscription Factor, residues 1-81)

PR-783 p53GST (mt, L22Q and W23S) 10 µg(Tumor Suppressor Protein and TranscriptionFactor, residues 1-81)

PR-765 pRB 5 µg(Retinoblastoma Protein, TumorSuppressor Protein, TranscriptionFactor and Proliferation Factor)

PR-808 RAD51 10 µg(RecA-like protein, recombinationrepair protein)

PR-766 WT-1 (+ KTS) 5 µg(The Wilms’ Tumor Suppressor andTranscription/Pre-mRNA Splicing Factor)

PR-767 WT-1 (- KTS) 5 µg(The Wilms’ Tumor Suppressor andTranscription/Pre-mRNA Splicing Factor)

� Transcription Factors – Activators (Contd.)



PR-740 BRG1 5 µg(Brahma-related Gene 1 Protein, wild type)

PR-741 BRG1-mt 5 µg(Brahma-related Gene 1 Protein, K798R)

PR-728 p52 10 µg(Transcriptional Coactivator)

PR-729 p75 10 µg(Transcriptional Coactivator and LensEpithelial Cell-Derived Growth Factor)

PR-758 p75-CTR 10 µg[C-terminal region of p75 (LEDGF)]

PR-762 p300 2 µg(Tumor Suppressor Protein andTranscription Factor)

� Transcription Factors – Coactivators

PR-770 p300 (C1135-2414) 4 µg(p300 C-terminus HAT Domain)

PR-725 PC4 10 µg(Positive Cofactor 4,Transcriptional Coactivator wild type)

PR-726 PC4-mt (F77P) 10 µg(Positive Cofactor 4, F77P mutant,Transcriptional Coactivator)

PR-727 PC4-mt (serine mutations) 10 µg(Positive Cofactor 4, serine mutations,Transcriptional Coactivator)

PR-735 Topo I 2 µg(Human DNA Topoisomerase I, wild type)

PR-736 Topo I CTD 2 µg[Human DNA Topoisomerase I, C-terminalDomain (CTD)]

PR-737 Topo I Core 2 µg(Human DNA Topoisomerase I, Core Domain)

PR-738 Topo I NTD 2 µg[Human DNA Topoisomerase I, N-terminalDomain (NTD)]

PR-739 Topo I (Y723F) 2 µg(Human DNA Topoisomerase I)

� Transcription Factors – Coactivators (Contd.)



PR-723 Dr1 10 µg(Down-regulator of transcription 1,NC2β, 19 kDa)

PR-800 Dr1GST 10 µg(Down-regulator of transcription 1,NC2β, 19 kDa)

PR-724 HMG1 10 µg(High Mobility Group 1)

PR-757 HMG1 10 µg(Native, High Mobility Group 1)Bovine, calf thymus

� Transcription Factors – Repressors

� Transcription Factors - Pre-mRNA SplicingFactors


PR-774 ASF/SF2 (SFRS1 or SRp30a) 10 µg(Splicing Factor, Arginine/Serine-rich 1,Pre-mRNA Splicing Factor) Human,

PR-775 ASF/SF2 (SFRS1 or SRp30a) 5 µg(Splicing Factor, Arginine/Serine-rich 1,Pre-mRNA Splicing Factor) Human,

PR-776 SC35 (SFRS2 or SRp30b) 5 µg(Splicing Component, 35 kDa,Pre-mRNA Splicing Factor) Human,

PR-766 WT-1 (+ KTS) 5 µg(The Wilms’ Tumor Suppressor andTranscription/Pre-mRNA Splicing Factor)

PR-767 WT-1 (- KTS) 5 µg(The Wilms’ Tumor Suppressor andTranscription/Pre-mRNA Splicing Factor)


Nuclear Receptors/HematopoetinsRECOMBINANT PROTEINSHematopoetins/Nuclear Receptor


PR-756 ERα α α α α (Estrogen Receptor, ααααα-isoform) 4 µg

PR-743 FXR (Farnesoid-X-activated Receptor) 10 µg

PR-804 FXRGST (Farnesoid-X-activated Receptor) 10 µg

PR-750 GR (Glucocorticoid Receptor) 5 µg

PR-744 LXR-ααααα (Liver-X Receptor, α-isoform) 10 µg

PR-805 LXR-αααααGST (Liver-X Receptor, α-isoform) 10 µg

PR-813 LXR-ααααα-N182GST 10 µg(Liver-X Receptor, α-isoform,N-terminal 182 region)

PR-746 LXR-ααααα-LBD 10 µg(Liver-X receptor, α-isoform,ligand binding domain)

PR-807 LXR-ααααα-LBDGST 10 µg(Liver-X Receptor, a-isoform,ligand binding domain)

PR-745 LXR-βββββ 10 µg(Liver-X Receptor, βββββ-isoform)

PR-806 LXR-βββββGST 10 µg(Liver-X Receptor, β-isoform)

PR-747 PPAR-ααααα 10 µg(Peroxisome Proliferator-activatedReceptor, α-isoform)

PR-814 PPAR-αααααGST 10 µg(Peroxisome Proliferator-activatedReceptor, α-isoform)

PR-816 PPAR-α-LBD 10 µg(Peroxisome Proliferator-activatedReceptor, a-isoform)

PR-815 PPAR-α-LBDGST 10 µg(Peroxisome Proliferator-activatedReceptor, a-isoform)

PR-748 PPAR-α 10 µg(Peroxisome Proliferator-activatedReceptor, α-isoform)

PR-822 PPAR-δδδδδ(βββββ)-LBDGST (residues 165-441) 10 µg(Peroxisome Proliferator-activatedReceptor, ααααα-isoform, ligandbinding domain)

PR-749 PPAR-γγγγγ 10 µg(Peroxisome Proliferator-activatedReceptor, γγγγγ-isoform)

PR-823 PPAR-γγγγγ-LBDGST (residues 204-477) 10 µg(Peroxisome Proliferator-activatedReceptor, γγγγγ-isoform, ligand binding domain)

PR-753 RAR-ααααα 5 µg(Retinoic Acid Receptor, α-isoform)

PR-819 RAR-ααααα-LBD (residues 154-462) 10 µg(Retinoic Acid Receptor, α-isoform,ligand binding domain)

PR-824 RAR-ααααα-LBDGST (residues 154-462) 10 µg

PR-820 RAR-βββββ-LBD (residues 173-409) 10 µg(Retinoic Acid Receptor, βββββ-isoform,ligand binding domain)

PR-825 RAR-βββββ-LBDGST (residues 173-409) 10 µg

PR-755 RAR-γγγγγ 5 µg(Retinoic Acid Receptor, γγγγγ-isoform)

� Nuclear Receptors


PR-812 RAR-γγγγγGST 10 µg(Retinoic Acid Receptor, γγγγγ-isoform)

PR-821 RAR-γγγγγ-LBD (residues 150-417) 10 µg(Retinoic Acid Receptor,γγγγγ-isoform, ligand binding domain)

PR-826 RAR-γγγγγ-LBDGST (residues 150-417) 10 µg

PR-721 RXR-ααααα 10 µg(Retinoid X Receptor, ααααα-isoform)

PR-742 RXR-ααααα-LBD (residues 200-462) 10 µg(Retinoid X Receptor, ααααα-isoform,Ligand Binding Domain)

PR-801 RXR-ααααα-LBDGST (residues 200-462) 10 µg(Retinoid X Receptor, ααααα-isoform,Ligand Binding Domain)

PR-752 RXR-βββββ 5 µg(Retinoid X Receptor, βββββ-isoform)

PR-802 RXR-βββββGST 10 µg

PR-751 TR-ααααα1 5 µg(Thyroid Hormone Receptor, ααααα1-isoform)

PR-803 TR-ααααα1GST 10 µg

PR-722 TR-ααααα1 10 µg(Thyroid Hormone Receptor, βββββ1-isoform)


PR-777 HeLa cell nuclear extract 200 µl(for in vitro transcription) Human

PR-778 HeLa cell nuclear extract 200 µl(for in vitro pre-mRNA splicing) Human

PR-779 293 cell nuclear extract 200 µl(for in vitro pre-mRNA splicing) Human

PR-780 HeLa cell cytosol extract 200 µl(S100) Human

� Nuclear Receptors (Contd.)

� Transcription Factors - Cell Extracts


PR-693 BMP-4 10 µg(Bone Morphogenetic Protein-4)

PR-402 EPO ααααα 500 U(Erythropoietin a)

PR-403 EPO βββββ 500 U(Erythropoietin βββββ)

PR-419 FLT3 Ligand 10 µg(Fms-like Tyrosine Kinase 3 Ligand)

PR-421 FLT3 Ligand 10 µg(Fms-like Tyrosine Kinase 3 Ligand) Murine,

PR-424 G-CSF(Granulocyte-Colony Stimulating 10 µgFactor) Human, Recombinant, CHO Cells

PR-423 G-CSF(Granulocyte-Colony Stimulating Factor) 10 µgHuman, Recombinant, E. coli

� Hematopoietins


Hematopoetins / CytokinesRECOMBINANT PROTEINS

Hematopoetins/Cytokines


PR-425 G-CSF, pegylated 10 µg

PR-436 GM-CSF(Granulocyte Macrophage-Colony 10 µgStimulating Factor)Human, Recombinant, E. coli

PR-437 GM-CSF(Granulocyte Macrophage-Colony 10 µgStimulating Factor)Murine, Recombinant, E. coli

PR-462 IL-3 10 µg

PR-471 IL-11 10 µg

PR-490 LIF 10 µg(Leukemia Inhibitory Factor)

PR-491 M-CSF 10 µg(Macrophage Colony Stimulating Factor)

PR-682 SCF (Stem Cell Factor) 10 µg

PR-683 SCF(Stem Cell Factor) 10 µgMouse, Recombinant, E. coli

PR-684 SCF (Stem Cell Factor) 10 µgRat, Recombinant, E. coli

� Hematopoietins (Contd.)


PR-443 IFN-ααααα 1b 100 µg

PR-439 IFN-ααααα 2a 100 µg

PR-440 IFN-ααααα 2a, pegylated 20 µg

PR-441 IFN-ααααα 2b 100 µg

PR-442 IFN-ααααα 2b, pegylated 20 µg

PR-444 IFN-ααααα 1ααααα 10 µg

PR-445 IFN-βββββ 1b 10 µg

PR-420 IFN-γγγγγ 50 µg

� Antiviral Cytokines


PR-497 Prolactin 50 µgHuman

PR-498 Prolactin 1 mgOvine (sheep)

PR-430 TNF ααααα 50 µg(Tumor Necrosis Factor)Human, Recombinant, E. coli

PR-431 TNF ααααα 50 µgMouse, Recombinant, E. coli

PR-685 TNF-βββββ 20 µgHuman, Recombinant, E. coli

PR-687 TPO 10 µg(Thrombopoietin, Megakaryocyte colonystimulating factor)Human, Recombinant, E. coli

PR-688 TPO 10 µgMouse, Recombinant, E. coli

� Apoptosis-inducing Cytokines


PR-407 BMP-2 (Bone Morphogenetic Protein-2) 10 µg

PR-408 BMP-7 (Bone Morphogenetic Protein-7) 10 µg

PR-463 IL-4 10 µg(Interleukin 4, B-cell stimulatory factor)Human

PR-464 IL-4 10 µg(Interleukin 4, B-cell stimulatory factor)Murine

PR-470 IL-10 (Interleukin 10) 10 µg

PR-477 Insulin 25 µg

PR-480 Leptin (Obesity Factor) 1 µg

PR-481 Leptin (Obesity Factor) 1 µgMurine, Recombinant, E. coli

PR-483 Leptin (Obesity Factor) 1 µgOvine (sheep), Recombinant, E. coli

PR-482 Leptin (Obesity Factor) 1 µgRat, Recombinant, E. coli

PR-484 Leptin, triple mutant (L39A, D40A, F41A) 100 µg(Obesity Factor)Human, Recombinant, E. coli

PR-485 Leptin, quadruple mutant (L39A, D40A, 100 µgF41A, I42A)(Obesity Factor) Human, Recombinant, E. coli

PR-486 Leptin, triple mutant (L39A, D40A, F41A) 100 µg(Obesity Factor)Murine, Recombinant, E. coli

PR-488 Leptin, triple mutant (L39A, D40A, F41A) 100 µg(Obesity Factor)Ovine (sheep), Recombinant, E. coli

PR-489 Leptin, quadruple mutant (L39A, D40A, 100 µgF41A, I42A) (Obesity Factor)Ovine (sheep), Recombinant, E. coli

PR-487 Leptin, triple mutant (L39A, D40A, F41A) 100 µg(Obesity Factor) Rat, Recombinant, E. coli

� Suppressors


� B-Cell Growth Factors


PR-406 BLyS/BAFF 20 µg(B Lymphocyte Stimulator/ B cell-activating factor belonging to the TNF family)Human, Recombinant, E. coli

PR-680 CD40L, soluble 50 µg(CD40 Ligand/CD154/TRAP TNF-related Activation Protein)Human, Recombinant, E. coli

PR-465 IL-5 Human, Recombinant, E. coli 10 µg


PR-467 IL-6 Murine, Recombinant, E. coli 10 µg

� T-Cell Growth Factors







� Proinflammatory Cytokines


PR-400 IL-1 a Human, Recombinant, E. coli 10 µg

PR-401 IL-1 ß Human, Recombinant, E. coli 10 µg

PR-459 IL-1 ß Murine, Recombinant, E. coli 10 µg

PR-460 IL-1ra (Interleukin 1 receptor antagonist)Human, Recombinant, E. coli 10 µg





PR-474 IL-15 Rat, Recombinant, E. coli 10 µg

PR-478 KGF-1 (Keratinocyte Growth Factor 1)Human, Recombinant, E. coli 10 µg

PR-479 KGF-2 (Keratinocyte Growth Factor 2)Human, Recombinant, E. coli 10 µg

PR-430 TNF ααααα Human, Recombinant, E. coli 50 µg

PR-431 TNF ααααα Mouse, Recombinant, E. coli 50 µg

PR-685 TNF-βββββ Human, Recombinant, E. coli 20 µg


PR-404 Artemin 10 µg

PR-405 BDNF 10 µg(Brain-derived Neurotrophic Factor)

PR-411 CNTF 20 µg(Ciliary Neurotrophic Factor)

PR-412 EG-VEGF 10 µg(Endocrine Gland-derived VascularEndothelial Growth Factor)

PR-413 EGF (Epidermal Growth Factor) 500 µgHuman, Recombinant, E. coli

PR-414 EGF (Epidermal Growth Factor) 50 µgHuman, Recombinant, Pichia pastoris

PR-809 FGF-1 (Acidic Fibroblast Growth Factor) 5 µgHuman, Recombinant, Sf9 insect cells

PR-415 FGF-1 (Fibroblast Growth Factor, acidic) 50 µgHuman, Recombinant, E. coli

PR-810 FGF-2 (Basic Fibroblast Growth Factor) 5 µgHuman, Recombinant, Sf9 insect cells

PR-416 FGF-2 (Fibroblast Growth Factor, basic) 50 µgHuman, Recombinant, E. coli

PR-417 FGF-2, bovine (Fibroblast Growth Factor, 50 µgbasic) Bovine, Recombinant, E. coli

PR-418 FGF-9 (Fibroblast Growth Factor-9) 10 µgMurine, Recombinant, E. coli

PR-422 GDNF (Glial-Derived Neurotrophic Factor) 10 µgHuman, Rec., E. coli

PR-435 GH (Growth Hormone) 20 µgBream, Recombinant, E. coli

PR-433 GH (Growth Hormone) 200 µgOvine (sheep), Recombinant, E. coli

PR-434 GH (Growth Hormone) 20 µgRat, Recombinant, E. coli

PR-428 GH-20K, placental 100 µg(Placental Growth Hormone-20K)

PR-427 GH-20K, pituitary 100 µg(Pituitary Growth Hormone-20K)

PR-429 GH-22K 100 µg(Placental Growth Hormone-22K)

PR-426 GH1, GH-22K 500 µg(Pituitary Growth Hormone)

PR-438 HGF (Hepatocyte Growth Factor) 10 µgHuman, Recomb., High 5 insect cells

PR-446 IGF-I (Insulin Like Growth Factor I, IGF-1) 100 µgHuman, Recombinant, E. coli

PR-451 IGF-I Barramundi, Recombinant, E. coli 20 µg

PR-453 IGF-I Bream, Recombinant, E. coli 20 µg

PR-449 IGF-I Chicken, Recombinant, E. coli 20 µg

PR-447 IGF-I Murine, Recombinant, E. coli 50 µg

� Other Growth Factors

Cytokines – Suppressors, Proinflammatory/ Growth FactorsRECOMBINANT PROTEINS

Cytokines/Growth Factors


Growth Factors - VEGF/Inhibitors/Ca2+ binding proteinsRECOMBINANT PROTEINS

Growth Factors


PR-452 IGF-I Salmon/Trout, Recombinant, E. coli 20 µg

PR-450 IGF-I Tuna, Recombinant, E. coli 20 µg

PR-448 IGF-I Rat, Recombinant, E. coli 20 µg

PR-454 IGF-II Human, Recombinant, E. coli 50 µg

PR-455 IGF-II Chicken, Recombinant, E. coli 20 µg

PR-456 IGFBP-1 (Insulin Like Growth Factor 25 µgBinding Protein 1) Human, Recombinant,mouse myeloma (NSO) cells

PR-457 IGFBP-2 Bovine, Recombinant, E. coli 25 µg

PR-458 IGFBP-3 Human, Recombinant, E. coli 25 µg

PR-478 KGF-1 (Keratinocyte Growth Factor 1) 10 µg

PR-479 KGF-2 (Keratinocyte Growth Factor 2) 10 µg

PR-409 NGF βββββ (Nerve Growth Factor ß) 20 µg

PR-493 NT-3, NGF-2 (Neurotrophin-3, Nerve 10 µgGrowth Factor 2)

PR-494 PDGF-AA (Platelet-derived Growth 10 µgFactor-AA)

PR-495 PDGF-AB (Platelet-derived Growth 10 µgFactor-AB)

PR-496 PDGF-BB (Platelet-derived Growth 10 µgFactor-BB)

PR-689 VEGF (Vascular Endothelial Growth Factor)Human, Recombinant, E. coli 10 µg

PR-691 VEGF (Vascular Endothelial Growth Factor)Bovine, Recombinant, E. coli 10 µg

PR-692 VEGF (Vascular Endothelial Growth Factor)Mouse, Recombinant, E. coli 10 µg

PR-690 VEGF-121 (Vascular EndothelialGrowth Factor variant)Human, Recombinant, E. coli 10 µg

� Other Growth Factors (Contd..)


PR-492 MIA (Melanoma Inhibitory Activity Protein)Human, Recombinant, E. coli 5 µg

� Growth Inhibitors

For TNF-α and β please view page #


PR-390 Calmodulin Human, Recombinant, E. coli 100 µg

PR-391 S100A1 (Calcium-binding Protein A1)Human, Recombinant, E. coli 50 µg




PR-392 Titin, I-band fragment Human,Recombinant, E. coli 100 µg

PR-393 Ventricular Myosin RegulatoryLight Chain LCIIHuman, Recombinant, E. coli 100 µg

PR-394 Non-muscle Myosin II RegulatoryLight Chain Human, Recombinant, E. coli 100 µg

� Calcium binding proteins


PR-150 Cu/Zn SODHis

(Cu/Zn Superoxide Dismutase) 1000 U

PR-149 Mn SODHis

(Manganese Superoxide Dismutase) 500 U

PR-130 MSRAHis

(Methionine sulfoxide reductase A,EC1.8.4.6) 100 µg

PR-131 MSRB1His

(Methionine sulfoxide reductase B1,EC1.8.4.6) 100 µg

PR-811 GSTGlutathione S-TransferaseSchistosoma japonicum, Recombinant, E. coli 5 µg

� Antioxidants


� Fmoc Labeled amino acids

• Fmoc Amino Acids – Ala

• Fmoc Amino Acids – Abu

• Fmoc Amino Acids – Arg

• Fmoc Amino Acids – Asn

• Fmoc Amino Acids – Asp

• Fmoc Amino Acids – Cys

• Fmoc Amino Acids – Glu

• Fmoc Amino Acids – Gln

• Fmoc Amino Acids – Gly

• Fmoc Amino Acids – His

• Fmoc Amino Acids – Ile

• Fmoc Amino Acids – Leu

• Fmoc Amino Acids – Lys

• Fmoc Amino Acids – Met

• Fmoc Amino Acids – Nle

• Fmoc Amino Acids – Nva

• Fmoc Amino Acids – Orn

• Fmoc Amino Acids – Phe

• Fmoc Amino Acids – Pro

• Fmoc Amino Acids – Ser

• Fmoc Amino Acids – Thr

• Fmoc Amino Acids – Tyr

• Fmoc Amino Acids - Val

• Fmoc Amino Acids – Unusual

� Boc Labeled Amino Acids

• Boc Amino Acids – Ala

• Boc Amino Acids – Arg

• Boc Amino Acids – Asn

• Boc Amino Acids – Asp

• Boc Amino Acids – Cys

• Boc Amino Acids – Glu

• Boc Amino Acids – Gln

• Boc Amino Acids – Gly

• Boc Amino Acids – His

• Boc Amino Acids – Ile

• Boc Amino Acids – Leu

• Boc Amino Acids – Lys

• Boc Amino Acids - Met

• Boc Amino Acids - Nle

• Boc Amino Acids – Nva

• Boc Amino Acids - Orn

• Boc Amino Acids - Phe

• Boc Amino Acids - Pro

• Boc Amino Acids - Ser

• Boc Amino Acids - Trp

• Boc Amino Acids – Tyr

• Boc Amino Acids – Val

• Boc Amino Acids – Unusual

Available in 1g, 5g and 25g pack sizes. Please enquire for pricing.

� Amino Acid Cartridges

� Fmoc L-Amino Acid Cartridges for ABISynthesizers (1 mmol)

Available in 1 & 10 Cartridges pack size (Enquire for Pricing)

� Fmoc D-Amino Acid Cartridges for ABISynthesizers (1 mmol)

Available in 1 & 10 Cartridges pack size (Enquire for Pricing)

� Fmoc L-Amino Acid Cartridges for Rainin PS-3Synthesizers (1 mmol)

Available in 10 Cartridges pack size (Enquire for Pricing)

� Fmoc L-Amino Acid Cartridges for Rainin PS-3Synthesizers (0.4 mmol)

Available in 10 Cartridges pack size (Enquire for Pricing)

� Boc L-Amino Acid Cartridges for ABI Synthesizers(1 mmol)

Available in 10 Cartridges pack size (Enquire for Pricing).

� Boc D-Amino Acid Cartridges for ABI Synthesizers(1 mmol)

Available in 1 & 10 Cartridges pack size (Enquire for Pricing).

� Boc L-Amino Acid Cartridges for ABI Synthesizers(2 mmol)


� Miscellaneous Amino Acid Cartridges for ABISynthesizers (1 mmol)


Fmoc and Boc Amino/Amino Acid catridgesPEPTIDE SYNTHESIS

Amino acids


� RESINS

� 2-Chlorotrityl Resins

Available in 1g & 5g pack sizes of different Amino Acids (Enquire forPricing)

� Trityl Resins

Available in 1g, 5g & 25 g of pack size (Enquire for Pricing)

• Diaminobutane trityl resin

• Trityl chloride resin

• 4-Methoxytrityl chloride resin

• Cysteamine 4-methoxytrityl resin

• Thiol 4-methoxytrityl resin

� Highly Substituted Resins

• Merrifield resin

• MBHA resin

• Fmoc-Rink Amide MBHA Resins.

� HMP Resins

Available in 1g & 5g pack sizes (Enquire for pricing)

� MAPS Resins

Fmoc and Boc resins available in 8-branch and 4-branch formats.

� PAM Resins

Available in 1g, 5g & 10g of pack sizes (Enquire for pricing).

� TentaGel Resins

Available in 1g & 5g of pack sizes (Enquire for pricing).

� Wang Resins

Available in 1g, 5g & 10g of pack sizes (Enquire for pricing).

� AFC, AMC, and pNA Resins

Available in 0.5g, & 1g of pack sizes (Enquire for pricing).

� Miscellaneous Resins

Available in 0.5g, 1g & 5g of pack sizes (Enquire for types and pricing).

� Coupling Reagents and Additives


21663 Benzotriazole-1-yl-oxy-tris- 5 gpyrrolidino-phosphoniumhexafluorophosphate



23510 BOP 25 g

29860 DCC 100 g

29861 DCC 500 g

25113 DIC 5 g

25114 DIC 25 g

25115 DIC 100 g

27221 DIEA 500 mL

27220 DIEA in NMP (2.0 M) 200 mL

23740 DIBOC 5 g

23741 DIBOC 25 g

23742 DIBOC 100 g

29883 DMAP 5 g

29884 DMAP 25 g

29858 DMAP 100 g

29859 DMAP 500 g

29855 EDC 5 g

29856 EDC 25 g

29857 EDC 100 g

21001 HBTU 100 g

21002 HBTU 500 g

27217 HBTU in HOBt (0.5 M) 200 mL

27256 HOAt 1 g

27255 HOAt 5 g

21003 HOBt, anhydrous 100 g

21004 HOBt, anhydrous 500 g

27218 Piperidine 200 mL

27219 Piperidine 500 mL

20376 TBTU 100 g

20377 TBTU 500 g

� Miscellaneous Reagents


20814 Fmoc-OSu 5 g

20815 Fmoc-OSu 25 g

20816 Fmoc-OSu 100 g

27257 HMPA (HMP Linker) 1 g

27258 HMPA (HMP Linker) 5 g

21392 Rink Amide Linker 1 g

24616 Rink Amide Linker 5 g

25241 Ninhydrin Test Kit 1 each

PEPTIDE SYNTHESISResins and reagentsResins/ Coupling Labeling Reagents


� Building Blocks


29271 (±)-N-4-Boc-2-methylpiperazine 1 g

29272 (±)-N-4-Boc-2-methylpiperazine 5 g

29273 tert-Butyl N-(2-aminoethyl)carbamate 1 g

29274 tert-Butyl N-(2-aminoethyl)carbamate 5 g

29275 tert-Butyl 1-piperazinecarboxylate 1 g

29276 tert-Butyl 1-piperazinecarboxylate 5 g

29277 (S)-N-4-Benzyl-2-isobutylpiperazine 1 g

29278 (S)-N-4-Benzyl-2-isobutylpiperazine 5 g

29279 (S)-N-1-Boc-2-isobutylpiperazine 1 g

29280 (S)-N-1-Boc-2-isobutylpiperazine 5 g

29281 (S)-N-4-Benzyl-2-isopropylpiperazine 1 g

29282 (S)-N-4-Benzyl-2-isopropylpiperazine 5 g

29283 (S)-N-1-Boc-2-isopropylpiperazine 1 g

29284 (S)-N-1-Boc-2-isopropylpiperazine 5 g

29285 (R)-N-4-Benzyl-2-phenylpiperazine 1 g

29286 (R)-N-4-Benzyl-2-phenylpiperazine 5 g

29287 (R)-N-4-Benzyl-2-(benzyloxymethyl) 1 gpiperazine

29288 (R)-N-4-Benzyl-2-(benzyloxymethyl) 5 gpiperazine

29289 (R)-N-1-Boc-2-(benzyloxymethyl)piperazine 1 g

29290 (R)-N-1-Boc-2-(benzyloxymethyl)piperazine 5 g

29291 (S)-N-4-Benzyl-2-benzylpiperazine 1 g

29292 (S)-N-4-Benzyl-2-benzylpiperazine 5 g

29293 (S)-N-1-Boc-2-benzylpiperazine 1 g

29294 (S)-N-1-Boc-2-benzylpiperazine 5 g

29295 (S)-N-4-Benzyl-2-(methylthioethyl)piperazine 1 g

29296 (S)-N-4-Benzyl-2-(methylthioethyl)piperazine 5 g

29297 (S)-N-4-Benzyl-2-(3-indolylmethyl)piperazine 1 g

29298 (S)-N-4-Benzyl-2-(3-indolylmethyl)piperazine 5 g

29299 (S)-1,4-Diazabicyclo[4.3.0]nonane 1 g

29300 (S)-1,4-Diazabicyclo[4.3.0]nonane 5 g

29301 (±)-1,4-Diazabicyclo[4.4.0]decane 1 g

29302 (±)-1,4-Diazabicyclo[4.4.0]decane 5 g

29315 trans-4-Hydroxy-L-proline methyl ester 5 ghydrochloride salt

29316 trans-4-Hydroxy-L-proline methyl 25 gester hydrochloride salt

29319 (R)-(-)-2-Hydroxy-4-phenylbutyric acid 5 g

29320 (R)-(-)-2-Hydroxy-4-phenylbutyric acid 25 g

29321 (1S,3R)-Methyl hydrogen cis-1,3- 5 gcyclopentanedicarboxylate

29322 (1S,3R)-Methyl hydrogen cis-1,3-cyclopentanedicarboxylate 25 g

29323 4-Amino-2-bromopyrimidine-5-carbonitrile 5 g

29324 4-Amino-2-bromopyrimidine-5-carbonitrile 25 g

� Building Blocks


29325 4-Amino-3-iodo-1H-pyrazolo 1 g[3,4-d]pyrimidine

29315 trans-4-Hydroxy-L-proline methyl 5 gester hydrochloride salt

29325 4-Amino-3-iodo-1H-pyrazolo[3,4-d] 1 gpyrimidine

29326 4-Amino-3-iodo-1H-pyrazolo[3,4-d] 5 gpyrimidine

29327 2-Chloro-4,6-dimethoxy-1,3,5-triazine (98%) 25 g

29328 2-Chloro-4,6-dimethoxy-1,3,5-triazine (98%) 25 g

29329 a- (p-Toluenesulfonyl)-4-fluorobenzylisonitrile 1 g

29330 a - (p-Toluenesulfonyl)-4- 5 gfluorobenzylisonitrile

60010-1 3-tert-Butoxycarbonylaminopiperidine 1 g




60012-1 N-1-Boc-3-aminopiperidine 1 g




60014-1 N-Boc-1,2,3,6-tetrahydropyridine 1 g

60014-5 N-Boc-1,2,3,6-tetrahydropyridine 5 g

60015-025 N-Boc-3-hydroxy-1,2,3,6- 0.25 gtetrahydropyridine

60015-1 N-Boc-3-hydroxy-1,2,3,6-tetrahydropyridine 1 g

60015-5 N-Boc-3-hydroxy-1,2,3,6-tetrahydropyridine 5 g

60016-1 N-Boc-trans-4-bromo-3-hydroxypiperidine 1 g

60016-5 N-Boc-trans-4-bromo-3-hydroxypiperidine 5 g

Building BlocksPEPTIDE SYNTHESIS

Building Blocks


••••• Amyloid Peptides

••••• Adrencorticotrophin peptides

••••• Agouti Related Peptides

••••• Angiotensins and Related Peptides

••••• Antibiotic Peptides

••••• Bombesins

••••• Osteocalcins

••••• Bradykinins

••••• Cell Permeable Peptides

••••• Calcitonin Related Peptides

••••• Endomorphins

••••• Cholecystokinin-Pancreozymin

••••• Endorphins

••••• Enkaphalins

••••• Corticotropin Releasing Factor

••••• Dynorphins

••••• Endothelins

••••• Leutenizing Hormone

••••• Endexins

••••• HIV Related Peptides

••••• Galanins

••••• Gastrins

••••• Fibronectin Fragments

••••• Glucagon-Like Peptides

••••• Growth Hormone Releasing Factors

••••• Matrix Metalloproteinases

••••• Neurokinins

••••• Natriuretic Peptides

••••• Orexins

••••• Neuromedins

••••• Neuropeptide Y and Analogs

••••• Neurotensins and Related Peptides

••••• Oxytocins, Vasopressins, and Related Peptides

••••• Pancreatic Polypeptides

••••• Parathyroid Hormones and Related Peptides

••••• Toxins

••••• Peptide YY and Analogs

••••• Prolactin Releasing Peptides

••••• Pituitary Adenylate Cyclase Activating Peptides (PACAPS)

••••• Proteolipid Proteins (PLPs)

••••• Secretins

••••• Somatostatins

••••• Substance P and Analogs

••••• Thrombin Related Peptides

••••• Thyrotropin Releasing Hormones and Related Peptides

••••• TNF Peptides

••••• Vasoactive Intestinal Peptides (VIPs)

••••• Viral Peptides

••••• Miscellaneous Bioactive Peptides

••••• Hepatitis-C Related Peptides

••••• Phosphopeptides

••••• Protein Kinase Related Peptides

••••• Apoptosis Blocking Peptides

••••• Cancer Research Blocking Peptides

••••• Signal Transduction Blocking Peptides

We have a broad range of Bioactive peptides for the categorieslisted. Please inquire for your specific requirement !

If not catalogued, we can custom synthesize it for you!

For Custom Peptide Synthesis please view page # 133

Bioactive PeptidesPEPTIDE SYNTHESIS

Catalogue Peptides


Gene Synthesis, Library constructionCUSTOM SERVICES

Genomic Services

� Construction of a Genomic DNA Lambda library

We can clone large inserts ranging in size from 9-23 kb into a lamdbavector. The Lambda DASH II Vector (Stratagene) will be used for ge-nomic lambda library construction. We do not guarantee average insertsize but will attempt to obtain desired range of average insert size.Insert size can vary from about 9-23Kb, as specified in manufactures’kit. We guarantee the minimum requested 1,000,000 clones.

Genomic library preparation highlights:

• DNA purification (>100kb in average).

• DNA random shearing by enzymatic methods.

• Size fractionation.

• Sticky-end ligation to the vector.

• Packaging and stock preparation.

• Customer will receive non-amplified phage stocks.

• Recombinant clones will be up to about 1 million.

� Construction of a Lambda cDNA library

cDNA libraries are constructed using our proprietary technology, whichfeatures full-length enriched, high quality size-fractionated, anddirectionally cloned cDNA. We do not guarantee average insert size butwill attempt to obtain desired range of average insert size. We guaran-tee the minimum requested 1,000,000 clones.

cDNA library preparation highlights:

• Total and polyA RNA isolation.

• 1st strand cDNA synthesis: 5’ adaptor primers will be added to

full-length cDNAs during this step; an oligo-dT or a random

primer based on the need of customer will be used.

• 2nd strand cDNA synthesis by primer extension.

• Double stranded cDNAs will be amplified using low cycling PCR if

necessary (optional).

• Size fractionation (normally recombinant clones will contain an

average insert size of at least 1kb).

• Ligation to phage vector.

• Packaging.

• Quality control (average insert size and non-insert clone

determination, number and percentage of recombinants).

• Customer will receive non-amplified cDNA stocks.

• Recombinant clones will be up to about 1 million for lambda.

Library amplification is available, however not included in this

offer.

� Plasmid DNA library (shotgun)

A modified pBluescript vector will be used. We do not guaranteeaverage insert size but will attempt to obtain desired range of averageinsert size. We guarantee the minimum requested 500,000 clones.

Service highlights:

• DNA purification (>100kb in average).

• DNA random shearing by chemical methods.

• Size fractionation.

• Blunt-end ligation to the vector.

• Recombinant clones will be up to about 500,000.

Clone array is optional.

� Customized Gene Synthesis

We offer custom gene synthesis service for a very economical prices.Turnaround time is dependent on sequence length. Progress report willbe issued at different stages. Confidentiality will be assured through anon-disclosure agreement (if necessary).

The following are included:

• Artificial gene synthesis;

• Expression optimization; Codon optimization (if necessary);

• Insertion of gene into standard plasmid or customer-

provided vector;

• Double stranded DNA sequencing data with chromatogram;

• The final product (10µg) can be delivered as a vector DNA

construct (vector map included) and/or as a lyophilized

clone mixture

• 100% fidelity

• Supplied as lyophilized plasmid

• E.coli culture transformed and stab provided at minimal

cost

••••• Expression in E.coli, Pichia pastoris, Baculovirus

� BAC genomic library

BAC libraries are essential elements in doing large-scale genome re-search such as genome sequencing, physical maps, and gene cloning.Bacterial artificial chromosomes (BAC) cloning technology was devel-oped in early 1990s and quickly gained popularity. This technology wasused in applications for genomic analysis that included large-scale physi-cal mapping and genomic sequencing although this technology was devel-oped much later than Yeast Artificial Chromosome (YAC) and cosmidcloning technologies.

• We guarantee coverage.

• We perform HMW DNA extraction ourselves.

• We have experience with plant, fungal, bacterial and animal

libraries.

• We have extensive experience with GC-rich organisms and

marine bacteria.

• Our arrayed BAC libraries are shipped in bar-coded

microplates.

• Shipment temperature of non-arrayed libraries is always

monitored using a BLUE FLAG Temperature Data logger.

• We provide technical support.

A few libraries constructed in the past include:

Starting material Insert size

Mouse tissue 180 Kb

Soy bean 150 Kb

Zeas Mays 150 Kb

Beetle 162 Kb

Canola plant 130 Kb

Pseudomonas sp. 140 Kb

Human liver 215 Kb

P. brassicacearum 160 Kb

P. omnivora 185 Kb

Arrayed, Non-arrayed, and screened libraries available.


Library Construction/ Site-directed Mutagenesis/Genome WalkingCUSTOM SERVICES

Genomic & Proteomic Services

� Plasmid cDNA library

cDNA libraries are produced using proprietary technology, which fea-tures full-length enriched, high quality size-fractionated, and directionallycloned cDNA. We do not guarantee average insert size but will attempt toobtain desired range of average insert size. We guarantee the minimumrequested 500,000 clones.

Service highlights:

• Competent cell preparation.

• cDNA synthesis to obtain full-length enriched cDNAs.

• Cloning will be directional.

• Size fractionation and ligation to vector (modified pBluescript).

• Transformation in E. coli DH10B.

• Plate will be identified with bar-coded labels.

• OPTIONAL: Arraying and Normalization

� Site-directed mutagenesis

• perform the site-directed mutagenesis and subsequent sequence

verification;

• provide purified plasmid DNA;

• provide E.coli strain harboring the plasmid with the target

mutation;

• provide a complete mutagenesis report.

Please provide us with 1mg of plasmid DNA with the target sequence,plasmid map, and last but not least, target sites for mutations! Plasmidmust be less than 8Kb. If greater than 8Kb a subcloning fees shall becharged extra.

� Other services:

• Subcloning

• Gap filling

• 5’ and 3’ RACE of first-strand cDNAs

• 5’ and 3’ genomic walking

• Identifying intron/exon junctions

• Identifying transgene/genomic DNA junctions

• Identifying gene traps and transposon-insertion location

• Bi-directional sequence extension of STSs and EST

� Custom Synthesis of Fluorescent Probes

We have extensive experience in custom-synthesizing biological stains,fluorescent and luminescent proves, peptides, nucleotides, carbohydratesand other biological molecules. With our state-of the art synthetic andanalytical instruments and GMP facilities, you can be assured that youwould get high quality products from us.

We offer custom conjugations for labeling your peptides, antibodies,proteins, nucleotides and nucleic acids with fluorescent dyes, lumines-cent probes, biotin, enzymes or other tagging molecules.

Custom Synthesis of Amino Acids and Peptides

We routinely perform difficult peptide synthesis such as synthesis oflong peptides (>50-mers), peptides containing unusual modifications andcyclic peptides. In addition, we can also do large-scale synthesis (>100g). Optimized Fmoc and Boc methodologies are employed in house toobtain high quality peptides. Although most of the peptides are purifiedby HPLC using reverse phase C

4 and C

18 columns, alternative purification

methods (e.g. ion exchange and gel filtration chromatography) are usedto purify some unique peptides. All custom peptides are accompaniedwith MS and HPLC analyses. Additional analytical data (e.g., sequencing,amino acid analysis) are available upon request.

We can synthesize modified peptides such as:

••••• DNA-peptide conjugates

••••• Drug-peptide conjugates

••••• Glycopeptides

••••• Lipopeptides

••••• Phosphopeptides

••••• Cyclic peptides (N -> C)

••••• Peptides with disulfide bonds

••••• Pegylated peptides

••••• Biotinylated peptides

••••• MAPS peptides

••••• Dye-labeled peptides:

••••• Peptides with C-terminal modification:

••••• Peptides with reduced amide bond

••••• Peptides labeled with C13 or N15

••••• Peptide libraries

Quantity range from 5mg to 100 mgPurity levels range from Crude to >98%Please enquire for bulk pricing

GMP-Grade Peptides

We synthesize GMA-grade peptides through our principals; AnaSpecInc., USA. Documentation and regulatory support are provided for yourclinical applications. AnaSpec holds a FDA Registration for GMP DrugManufacturing (Registration #2951078).

The fluorescent probes/dyes available are:

Fluoresceins Rhodamines Coumarins

FMA/FITC TAMRA/ROX AFC/AMC

JOE/HEX/TET Rh110/Rh123 Psoralens

FDG/ FDP Resorufin SPQ/MEQ

BCECF Carbocyanines NBD

Luciferins JC-1/Dil/Dio Lucifer Yellow

Coelenterazines DiBAC/DiSBAC Acrylodan

Dioxetanes Indo/Fura ATP/dUTP

BAPTA EGTA AMP/GMP

Fluoresceins Rhodamines Coumarins

DAPI Phospholipids Transferrin

Hoechst Ceramides Phycoerythrins

Nucleotides Peptides Avidins

Propidium Glycosides Biotins

Acridines Tetradotoxins Antibodies

Verapamil Bungarotoxins Microspheres

Biamines Taxols Phalloidins

Ryanodines Neuropeptides DNA/RNA


Custom Production of Monoclonal and Polyclonal AntibodiesCUSTOM SERVICES

Antibody and Other Services

� Polyclonal Antibody Production

Polyclonal Antibody Production for 2 rabbits:

• NIH 90-day standard protocol or 70-day conventional

protocol

• 10 mL pre-immune serum

• 100 mL anti-serum

Additional antibody services include:

• ELISA

• Peptide Synthesis 10 mg peptide, >80% purity, MS

and HPLC certified

• Peptide-Carrier Protein Conjugation to BSA or

KLH

� Complete Anti-Peptide Antibody Package includes:

• Free consultation for selection of epitope

• 5 mg purified (>80% purity, up to 15 amino acids) peptide

delivered to researcher for immediate study

• 1 mg conjugated to BSA for ELISA screening; 4 mg conju-

gated to KLH for antibody production

• Immunization of rabbits, mice, or chickens at your

preference

• Peptide-specific ELISA titration of antibody for all bleeds

• Pre-immune samples of serum, ascites, or eggs

• Shipment of 100 mL anti-serum, 20 mL ascites, or 12

antibody-containing eggs

� Monoclonal Antibody Production

• Shipment of 100 mL anti-serum, 20 mL ascites, or 12 antibody-

containing eggs

• Free consultation for epitope selection

• Evaluation of suitable screening methods

• Frequent interactions between researcher and AnaSpec during

hybridoma development process

• Project segmentation into different phases to provide

researcher flexibility for terminating project at the end of

each phase

• Full confidentiality assured

Phase I: Immunogen Design and Immunization (9 wks)

• Design and preparation of protein antigen by researcher or

peptide immunogen

• Site-directed conjugation for peptide including peptide-KLH

as antigen and peptide-BSA as screening reagent

• Immunization of 6 Balb/C mice, including maintenance and 3

immunizations for a standard 60-day protocol

• Determination and qualification of titer of test bleed (e.g.,

ELISA, Western Blot, IP)

• Shipment of all 6 mice test bleed at 1:100 dilution (1 mL each)

Phase II: Cell Fusion and Parental Clone Screening (3 wks)

• Mouse with the highest titer given final boost to establish

murine myeloma cell line

• Fused cell clones screened by ELISA to select positive

antibody-secreting parental clones

• Potential parental clones expanded into 24-well plates for

harvest of tissue culture supernatant for further antibody

screening (ELISA, Western Blot, or IHC)

• Shipment of 5-12 supernatants (2 mL/clone)

Phase III: Monoclonal Selection and Cryogenic Vial Preservation (3 wks)

• Monoclonal selection performed with 1-5 parental clones

• Subclone with high specificity expanded and cryopreserved

(4-5 vials each)

• Shipment of hybridoma cells and up to 10 mL of supernatant

from each subclone

Phase IV: Large Scale Antibody Production (3 wks)

*Production performed either in vivo (ascites) or in vitro

(bioreactor, stationary cell culture).

� Cell-Based Assays

There are a variety of fluorescent dyes for monitoring cell functions.Using our superior fluorescent dyes, the following cell-based assays areprovided:

• Cytotoxicity

• Cell Proliferation

• Cell viability

• Cell adhesion

• Muti-drug resistance

� Analytical Services

To accelerate customer’s research and development projects, we offera variety of analytical services besides our specialized custom servicesdescribed above.

• Small biomolecules

• Proteins

• Oligosaccharides

• Oligonucleotides

• Glycoproteins

• Lipids

• Other biopolymers


Reactive Fluorescent DyesFLUORESCENCE

Fluorescent Dyes

� Fluorescent Dyes


81401 ABD-F, [4-Fluoro-7-aminosulfonyl 10 mgbenzofurazan] λ

abs : 315 nm; λ

em: none

81207 AMCA-X, [6-((7-Amino-4-methylcoumarin- 25 mg3-acetyl)amino)hexanoic acid]λ

abs : 353 nm; λ

em: 442 nm

81208 AMCA-X, SE , [6-((7-Amino-4-methyl 10 mgcoumarin-3-acetyl)amino)hexanoic acid,succinimidyl ester] λ

abs : 442 nm; λ

em: 354 nm

81513 2-Aminoacridone 25 mgλ

abs : 425 nm (pH.7); λ

em: 532 nm (pH.7)

81512 AMF, 4'-(Aminomethyl)fluorescein, 25 mghydrochloride λ

abs : 493 nm; λ

em: 516 nm

81522 1-Aminomethylpyrene, hydrochloride 100 mgλ

abs : 340 nm; λ

em: 376 nm

81529 ANDS, [7-Aminonaphthalene-1,3-disulfonic 1 gacid, potassium salt]λ

abs : 350 nm; λ

em: 450 nm

81528 ANTS, [8-Aminonaphthalene-1,3,6-trisulfonic 100 mgacid, disodium salt] *UltraPure Grade*λ

abs : 353 nm; λ

em: 520 nm

81523 APTS, [8-Aminopyrene-1,3,6-trisulfonic 10 mgacid, trisodium salt]λ

abs : 424 nm; λ

em: 505 nm

81420 Badan, [6-Bromoacetyl-2-dimethyl amino 10 mgnaphthalene] λ

abs : 387 nm; λ

em: 520 nm

81448 bBBr, [Dibromobimane] *UltraPure Grade* 25 mgλ

abs : 395 nm; λ

em: 490 nm

81433 mBBr, [Monobromobimane] 25 mg*UltraPure Grade* λ

abs : 395 nm; λ

em: 490 nm

81015 5-(and-6)-Carboxy-2',7'-dichlorofluorescein 100 mgλ

abs : 504 nm; λ

em: 529 nm

81016 5-(and-6)-Carboxy-2',7' dichloro fluorescein, 25 mgsuccinimidyl esterλ

abs : 504 nm; λ

em: 529 nm

81131 5(6)-CR110, [5-(and-6)-Carboxyrhodamine 25 mg110, hydrochloride]λ

abs : 498 nm; λ

em: 521 nm

81132 5-CR110, [5-Carboxyrhodamine 110, 5 mghydrochloride] λ

abs : 498 nm; λ

em: 521 nm

81133 6-CR110, [6-Carboxyrhodamine 110, 5 mghydrochloride] λ

abs : 498 nm; λ

em: 521 nm

81134 5(6)-CR110, SE, [5-(and-6)- 5 mgCarboxyrhodamine 110, succinimidyl ester]λ

abs : 498 nm; λ

em: 521 nm

81135 5-CR110, SE, [5-Carboxyrhodamine 110, 5 mgsuccinimidyl ester]λ

abs : 498 nm; λ

em: 521 nm

81136 6-CR110, SE, [6-Carboxyrhodamine 110, 5 mgsuccinimidyl ester]λ

abs : 498 nm; λ

em: 521 nm

81101 5(6)-CR6G, [6-Carboxyrhodamine 6G, 25 mghydrochloride] λ

abs: 519 nm; λ

em: 544 nm

81102 5-CR6G, [5-(and-6)-Carboxyrhodamine 6G, 10 mghydrochloride] λ

abs: 518 nm; λ

em: 544 nm

81103 6-CR6G, [5-Carboxyrhodamine 6G, 10 mghydrochloride] λ

abs: 520 nm; λ

em: 547 nm

� Fluorescent Dyes (Contd.)


81104 5(6)-CR6G, SE, [5-(and-6)-Carboxyrhodamine 6G, 10 mgsuccinimidyl ester] λ

abs: 522 nm; λ

em: 550 nm

81105 5-CR6G, SE, [5-Carboxyrhodamine 6G, 5 mgsuccinimidyl ester] λ

abs: 524 nm; λ

em: 556 nm

81106 6-CR6G, SE, [6-Carboxyrhodamine 6G, 5 mgsuccinimidyl ester] λ

abs: 524 nm; λ

em: 551 nm

81402 DACIA, [N-(7-Dimethylamino-4-methylcoumarin- 10 mg3-yl)iodoacetamide] λ

abs: 376 nm; λ

em: 465 nm

81216 DACITC, [7-Dimethylamino-4-methylcoumarin- 10 mg3-isothiocyanate] λ

abs: 400 nm; λ

em: 476 nm

81403 DACM, [N-(7-Dimethylamino-4-methylcoumarin- 10 mg3-yl)maleimide] λ

abs: 383 nm; λ

em: 463 nm

81501 Dansyl cadaverine, [5-Dimethylaminonaphthalene 25 mg-1-(N-(5-aminopentyl))sulfonamide]λ

abs: 333 nm; λ

em: 518 nm

81201 Dansyl chloride [5-Dimethylaminonaphthalene- 100 mg1-sulfonyl chloride],λ

abs: 372 nm; λ

em: none

81209 Dansyl-X, acid λabs

: 333 nm; λem

: 518 nm 100 mg

81214 Dansyl-X, SE λabs

: 333 nm; λem

: 518 nm 25 mg

81422 DCIA, [7-Diethylamino-3-((4'-(iodoacetyl) 25 mgamino)phenyl) -4-methylcoumarin]λ

abs: 384 nm; λ

em: 470 nm

81210 DEAC, acid, [7-Diethylaminocoumarin- 250 mg3-carboxylic acid] λ

abs: 432 nm; λ

em: 472 nm

81211 DEAC, SE, [7-Diethylaminocoumarin-3- 25 mgcarboxylic acid, succinimidyl ester]λ

abs: 432 nm; λ

em: 472 nm

81225 DMACA, [7-Dimethylaminocoumarin- 100 mg4-acetic acid] λ

abs: 370 nm; λ

em: 459 nm

81226 DMACA, SE, [7-Dimethylaminocoumarin- 25 mg4-acetic acid, succinimidyl ester]λ

abs: 376 nm; λ

em: 468 nm

81001 5-DTAF, [5-(4,6-Dichlorotriazinyl) 25 mgaminofluorescein]λ

abs: 492 nm; λ

em: 517 nm

81012 6-DTAF [6-(4,6-Dichlorotriazinyl) 25 mgaminofluorescein]λ

abs: 492 nm; λ

em: 517 nm

23887 EDANS, acid, [5-((2-Aminoethyl)amino) 1 gnaphthalene-1-sulfonic acid] λ

abs: 335 nm;

λem

: 493 nm

81431 EDANS Iodoacetamide, 100 mg[5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid]λ

abs: 336 nm; λ

em: 490

81432 EDANS C2 maleimide λabs

: 336 nm; λem

: 490 25 mg

81535 EDANS, sodium salt, [5-((2 1 gAminoethyl)amino)naphthalene-1-sulfonic acid,sodium salt] λ

abs: 335 nm; λ

em: 493

81002 5(6)-FAM, [5-(and-6)-Carboxyfluorescein] 250λ

abs: 494 nm (pH>7.0); λ

em: 519 nm (pH>7.0) mg



Fluorescent Dyes

� Fluorescent Dyes (Contd.)


23887 EDANS, acid, [5-((2-Aminoethyl) 1 gamino)naphthalene-1-sulfonic acid]λ

abs: 335 nm; λ

em: 493 nm

81431 EDANS Iodoacetamide, 100 mg[5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid]λ

abs: 336 nm; λ

em: 490

81432 EDANS C2 maleimide 25 mgλ

abs: 336 nm; λ

em: 490

81535 EDANS, sodium salt, 1 g[5-((2-Aminoethyl)amino) naphthalene-1-sulfonic acid, sodium salt] λ

abs: 335 nm; λ

em: 493

81003 5-FAM, [5-Carboxyfluorescein] 100 mgλ

abs: 492 nm (pH>7.0); λ

em: 518 nm (pH>7.0)

81004 6-FAM, [6-Carboxyfluorescein] 100 mgλ

abs: 495 nm (pH>7.0); λ

em: 517 nm (pH>7.0)

81502 5-FAM cadaverine, 10 mg[Fluorescein-5-carboxamide cadaverine]λ

abs: 494 nm (pH>7.0); λ

em: 521 nm (pH>7.0)

81503 5-FAM LYS, [Fluorescein-5-carboxamide lysine] 10 mgλ

abs: 494 nm (pH>7.0); λ

em: 521 nm (pH>7.0)

81006 5(6)-FAM, SE, [5-(and-6)- 25 mgCarboxyfluorescein, succinimidyl ester]λ

abs: 494 nm (pH>7.0); λ

em: 519 nm (pH>7.0)

81007 5-FAM, SE, [5-Carboxyfluorescein, 10 mgsuccinimidyl ester]λ

abs: 492 nm (pH>7.0); λ

em: 518 nm (pH>7.0)

81008 6-FAM, SE, [6-Carboxyfluorescein, 10 mgsuccinimidyl ester]λ

abs: 495 nm (pH>7.0); λ

em: 517 nm (pH>7.0)

81009 5-FAM-X, SE, [6-(Fluorescein-5- 5 mgcarboxamido)hexanoic acid, succinimidyl ester]λ

abs: 494 nm (pH>7.0); λ

em: 521 nm (pH>7.0)

81504 5-FITC cadaverine, 5 mg[5-((5-Aminopentyl)thioureidyl)fluorescein]λ

abs: 492 nm (pH>7.0); λ

em: 516 nm (pH>7.0)

81005 FITC ‘Isomer I’, [5-FITC; 100 mgfluorescein-5-isothiocyanate]λ

abs: 494 nm (pH>7.0); λ

em: 519 nm (pH>7.0)

81010 FITC ‘Isomer II’, [6-FITC, 100 mgfluorescein-6-isothiocyanate]λ

abs: 494 nm (pH>7.0); λ

em: 520 nm (pH>7.0)

81202 Fluorescamine *UltraPure Grade* 25 mgλ

abs: 315 nm; λ

em: None

81405 Fluorescein-5-maleimide 25 mgλ

abs: 493 nm; λ

em: 515 nm

81505 5-FTSC, [Fluorescein-5-thiosem

icarbazide] 25 mgλ

abs: 492 nm (pH>7.0); λ

em: 516 nm (pH>7.0)

81019 6-HEX, acid, [6-Carboxy-2',4,4',5',7,7'- 25 mghexachlorofluorescein]λ

abs: 533 nm; λ

em: 550 nm

81020 6-HEX, SE, [6-Carboxy-2',4,4',5',7,7'- 5 mghexachlorofluorescein, succinimidyl ester]λ

abs: 533 nm; λ

em: 550 nm

81160 HiLyte Fluor™ 488 acid (can be substituted 10 mgfor FITC) λ

abs: 497 nm; λ

em: 525 nm

81250 HiLyte Fluor™ 555 acid 10 mg(can be substituted for Cy3 dye)λ

abs: 550 nm; λ

em: 566 nm

81255 HiLyte Fluor™ 647 acid (can be substituted for 5 mgCy5 dye) λ

abs: 650 nm; λ

em: 675 nm

� Fluorescent Dyes (Contd..)


81260 HiLyte Fluor™ 680 acid (can be substituted 5 mgfor Cy5.5 dye) λ

abs: 678 nm; λ

em: 699 nm

81265 HiLyte Fluor™ 750 acid 5 mg(can be substituted for Cy7 dye)λ

abs: 753 nm; λ

em: 778 nm

81161 HiLyte Fluor™ 488 acid, SE 5 mg(can be substituted for FITC)λ

abs: 498 nm; λ

em: 525 nm

81251 HiLyte Fluor™ 555 acid, SE 5 mg(can be substituted for Cy3 dye)λ

abs: 552 nm; λ

em: 569 nm


abs: 649 nm; λ

em: 674 nm

81261 HiLyte Fluor™ 680 acid, SE 1 mg(can be substituted for Cy5.5 dye)λ

abs: 678 nm; λ

em: 699 nm


abs: 754 nm; λ

em: 778 nm

81162 HiLyte Fluor™ 488 amine 1 mg(can be substituted for FITC)λ

abs: 499 nm; λ

em: 526 nm

81252 HiLyte Fluor™ 555 amine 1 mg(can be substituted for Cy3 dye)λ

abs: 551 nm; λ

em: 567 nm


abs: 649 nm; λ

em: 674 nm

81262 HiLyte Fluor™ 680 amine 1 mg(can be substituted for Cy5.5 dye)λ

abs: 678 nm; λ

em: 699 nm


abs: 754 nm; λ

em: 778 nm

81164 HiLyte Fluor™ 488 C2 maleimide 1 mg(can be substituted for FITC)λ

abs: 498 nm; λ

em: 525 nm

81254 HiLyte Fluor™ 555 C2 maleimide 1 mg(can be substituted for Cy3 dye)λ

abs: 552 nm; λ

em: 569 nm


abs: 649 nm; λ

em: 674 nm

81264 HiLyte Fluor™ 680 C2 maleimide 1 mg(can be substituted for Cy5.5 dye)λ

abs: 678 nm; λ

em: 699 nm


abs: 754 nm; λ

em: 778 nm

81163 HiLyte Fluor™ 488 hydrazide 1 mg(can be substituted for HRP)λ

abs: 499 nm; λ

em: 526 nm

81253 HiLyte Fluor™ 555 hydrazide 1 mg(can be substituted for Cy3 dye)λ

abs: 551 nm; λ

em: 567 nm

81258 HiLyte Fluor™ 647 hydrazide (can be substituted 1 mgfor Cy5 dye) λ

abs: 649 nm; λ

em: 674 nm

81263 HiLyte Fluor™ 680 hydrazide (can be substituted 1 mgfor Cy5.5) λ

abs: 678 nm; λ

em: 699 nm

81268 HiLyte Fluor™ 750 hydrazide (can be substituted 1 mgfor Cy7 dye) λ

abs: 753 nm; λ

em: 782 nm

81205 7-Hydroxycoumarin-3-carboxylic acid 250 mgλ

abs: 387 nm; λ

em: 448 nm



81206 7-Hydroxycoumarin-3-carboxylic acid, 50 mgsuccinimidyl esterλ

abs: 363 nm; λ

em: 447 nm

81235 7-Hydroxy-4-methylcoumarin-3-acetic acid 100 mgλ

abs: 360 nm; λ

em: 455 nm

81239 7-Hydroxy-4-methylcoumarin-3- 25 mgacetic acid, succinimidyl esterλ

abs: 364 nm; λ

em: 458 nm

81406 5-IAF, [5-Iodoacetamidofluorescein] 25 mgλ

abs: 493 nm; λ

em: 515 nm

81425 IANBD amide, [N,N’-Dimethyl-N- 25 mg(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine]λ

abs: 478 nm; λ

em: 541 nm

81421 IDCC, [7-Diethylamino-3- 25 mg((((2-iodoacetamido)ethyl)amino)carbonyl)coumarin]λ

abs: 420 nm; λ

em: 470 nm

81011 6-JOE, SE, [6-Carboxy-4',5'-dichloro-2',7'- 5 mgdimethoxyfluorescein, succinimidyl ester]λ

abs: 520 nm (pH > 9.0); λ

em: 548 nm (pH > 9.0)

81530 LRB-EDA, [Lissamine™ rhodamine 10 mgB ethylenediamine]λ

abs: 560 nm; λ

em: 581 nm

81108 LRB-SC, [Lissamine™ rhodamine B 100 mgsulfonyl chloride]λ

abs: (in dichloromethane): 568 nm;

λem

: (in dichloromethane): 584 nm

81240 7-Methoxycoumarin-3-carbonyl azide 25 mgλ

abs: 360 nm; λ

em: 415 nm

81241 7-Methoxycoumarin-3-carboxylic acid 250 mgλ

abs: 336 nm; λ

em: 402 nm

81242 7-Methoxycoumarin-3-carboxylic acid, 100 mgsuccinimidyl esterλ

abs: 358 nm; λ

em: 410 nm

81203 NBD-Cl [4-Chloro-7-nitrobenzofurazan] 25 mg*UltraPure Grade*λ

abs: 337 nm; λ

em: none

81204 NBD-F, [4-Fluoro-7-nitrobenzofurazan] 25 mgλ

abs: 337 nm; λ

em: none

81516 NBD lysine 25 mgλ

abs: 466 nm; λ

em: 533

81212 NBD-X, [6-(N-(7-Nitrobenz-2-oxa-1, 100 mg3-diazol-4-yl)amino)hexanoic acid]λ

abs: 467 nm; λ

em: 539

81213 NBD-X, SE, [Succinimidyl 6- 25 mg(N-(7-nitrobenz-2-oxa-1,3- diazol-4-yl)amino)hexanoate] λ

abs: 466 nm; λ

em: 535

81236 1-Pyrenebutanoic acid 100 mgλ

abs: 341 nm; λ

em: 376

81237 1-Pyrenebutanoic acid, hydrazide 100 mgλ

abs: 341 nm; λ

em: 376

81238 1-Pyrenebutanoic acid, succinimidyl ester 100 mgλ

abs: 340 nm; λ

em: 376

81436 N-(1-Pyrene)maleimide 100 mgλ

abs: 338 nm; λ

em: 375

81435 PMIA, N-(1-Pyrenλem

ethyl) iodoacetamide 100 mgλ

abs: 338 nm (cysteine adduct);


81244 1-Pyrenesulfonyl chloride 100 mgλ

abs: 350 nm (cysteine adduct);

λem

: 380 nm (cysteine adduct)

81110 5(6)-ROX, [5-(and-6)-Carboxy-X-rhodamine] 100 mgλ

abs: 568 nm; λ

em: 591nm

81111 5-ROX, [5-Carboxy-X-rhodamine] 10 mgλ

abs: 567 nm; λ

em: 591nm

81112 6-ROX, [6-Carboxy-X-rhodamine] 10 mgλ

abs: 570 nm; λ

em: 591nm

81113 5(6)-ROX, SE, [5-(and-6)- 25 mgCarboxy-X-rhodamine, succinimidyl ester]λ

abs: 576 nm; λ

em: 601 nm

81114 5-ROX, SE, [5-Carboxy-X-rhodamine, 5 mgsuccinimidyl ester] λ

abs: 573 nm; λ

em: 602 nm

81115 6-ROX, SE, [6-Carboxy-X-rhodamine, 5 mgsuccinimidyl ester] λ

abs: 575 nm; λ

em: 602 nm

81408 SBF-Cl, [4-Chloro-7-sulfobenzofurazan, 25 mgammonium salt] λ

abs: 380 nm; λ

em: none

81409 SBF-F, [4-Fluoro-7-sulfobenzofurazan, 10 mgammonium salt] λ

abs: 385 nm; λ

em: none

81447 Sulforhodamine 101 C2 maleimide 5 mgλ

abs: 588 nm; λ

em: 601 nm

81510 Sulforhodamine 101 cadaverine 5 mgλ

abs: 583 nm; λ

em: 601 nm

81540 Sulforhodamine 101 hydrazide 5 mgλ

abs: 583 nm; λ

em: 601 nm

81511 Sulforhodamine 101 lysine 5 mgλ

abs: 583 nm; λ

em: 600 nm

81130 Sulforhodamine 101 sulfonyl chloride 10 mgλ

abs: 588 nm; λ

em: 601 nm

81120 5(6)-TAMRA, [5-(and-6)- 25 mgCarboxytetramethylrhodamine]λ

abs: 541 nm; λ

em: 565 nm

81121 5-TAMRA, [5-Carboxytetramethylrhodamine] 10 mgλ

abs: 541 nm; λ

em: 568 nm

81122 6-TAMRA, [6-Carboxytetramethylrhodamine] 10 mgλ

abs: 541 nm; λ

em: 568 nm

81506 5(6)-TAMRA cadaverine, 10 mg[Tetramethylrhodamine 5-(and -6)-carboxamide cadaverine]λ

abs: 544 nm; λ

em: 570 nm

81507 5-TAMRA cadaverine, 5 mg[Tetramethylrhodamine-5-carboxamidecadaverine] λ

abs: 545 nm; λ

em: 576 nm

81508 6-TAMRA cadaverine, 5 mg[Tetramethylrhodamine 6-carboxamidecadaverine] λ

abs: 544 nm; λ

em: 575 nm

81509 5-TAMRA Lysine, [Tetramethylrhodamine-5- 5 mgcarboxamide lysine]

λ

abs: 545 nm; λ

em: 577 nm

81124 5(6)-TAMRA, SE, [5-(and-6)- 25 mgCarboxytetramethylrhodamine,succinimidyl ester] λ

abs: 546 nm; λ

em: 575 nm

81125 5-TAMRA, SE [5-Carboxytetramethylrhodamine, 5 mgsuccinimidyl ester], λ

abs: 547 nm; λ

em: 574 nm

� Fluorescent Dyes (Contd...) � Fluorescent Dyes (Contd...)


Fluorescent Dyes


� Fluorescent Dyes (Contd...)


81126 6-TAMRA, SE, [6-Carboxytetramethylrhodamine, 5 mgsuccinimidyl ester]λ

abs: 547 nm; λ

em: 573 nm

81127 5(6)-TAMRA-X, SE, 5 mg[6-(Tetramethylrhodamine-5-(and-6)-carboxamido) hexanoic acid, succinimidyl ester]λ

abs: 544 nm; λ

em: 572 nm

81021 6-TET, acid, [6-Carboxy-2',4,7,7'- 25 mgtetrachlorofluorescein]λ

abs: 520 nm; λ

em: 535 nm

81022 6-TET, SE, [6-Carboxy-2',4,7,7'- 5 mgtetrachlorofluorescein, succinimidyl ester]λ

abs: 521 nm; λ

em: 536 nm

81444 Tetramethylrhodamine-5- 25 mg(and-6)-maleimide,λ

abs: 540 nm; λ

em: 567 nm

81446 Tetramethylrhodamine-5-maleimide 5 mgλ

abs: 540 nm; λ

em: 567 nm

81445 Tetramethylrhodamine-6-maleimide 5 mgλ

abs: 542 nm; λ

em: 568 nm

81410 5-TMRIA, [Tetramethylrhodamine-5- 5 mgiodoacetamide]λ

abs: 541 nm; λ

em: 567 nm

81150 5(6)-TRITC, [Tetramethylrhodamine-5- 10 mg(and-6)-isothiocyanate]λ

abs: 543 nm; λ

em: 571 nm

81151 5-TRITC; G isomer, 5 mg[Tetramethylrhodamine-5-isothiocyanate]λ

abs: 543 nm; λ

em: 571 nm

81152 6-TRITC; R isomer , 5 mg[Tetramethylrhodamine-6-isothiocyanate]λ

abs: 544 nm; λ

em: 572 nm


83213 ACMA 25 mg[9-Amino-6-chloro-2-methoxyacridine]λ

abs: 412 nm; λ

em: 471 nm

83298 Acridine orange 1 gλ

abs: 500 nm; λ

em: 526 nm

83299 Acridine orange, 10 mg/mL solution 10 mLin water, λ

abs: 500 nm; λ

em: 526 nm

83300 Acridine orange 10-nonyl bromide 25 mg[Nonyl acridine orange]λ

abs: 495 nm; λ

em: 519 nm

83200 Actinomycin D 5 mgλ

abs: 442 nm; λ

em: none

83210 DAPI 10 mg[4'’,6-Diamidino-2-phenylindole,dihydrochloride]λ

abs: 358 nm; λ

em: 461 nm

83211 DAPI 25 mg[4'’,6-Diamidino-2-phenylindole,dihydrochloride] *Custom Packaging*λ

abs: 358 nm; λ

em: 461 nm

85704 Dihydroethidium [Hydroethidine] 25 mgλ

abs: 355 nm; λ

em: N/A

85718 Dihydroethidium [Hydroethidine], 1 mL5 mM solution in DMSOλ

abs: 355 nm; λ

em: N/A

85718 Dihydroethidium [Hydroethidine] 10 mL*Special Air-free Packaging*λ

abs: 355 nm; λ

em: N/A

83219 Hoechst 33258, 20 mM solution in water 5 mLλ

abs: 352 nm; λ

em: 461 nm

83218 Hoechst 33342, 20 mM solution in water 5 mLλ

abs: 350 nm; λ

em: N/A

83216 Hoechst 34580 5 mgλ

abs: 392 nm; λ

em: 440 nm

83239 Methylene Blue *UltraPure Grade* 1 gλ

abs: 661 nm; λ

em: N/D

83238 Methyl Green *UltraPure Grade* 1 gλ

abs: 635 nm; λ

em: N/D

83237 Pyronin Y *UltraPure Grade* 100 mgλ

abs: 546 nm; λ

em: 549 nm

83227 Thiazole Orange *UltraPure Grade* 100 mgλ

abs: 512 nm; λ

em: 533 nm

83228 Thiazole Orange, 10 mM in DMSO 10 mL*UltraPure Grade*λ

abs: 512 nm; λ

em: 533 nm

� Fluorescent Probes for detecting Nucleic acids-Cell permeant Atains

Reactive Fluorescent Dyes, Fluor. Probes for Nucleic AcidsFLUORESCENCE

Fluorescent Dyes/ Probes

� Fluorescent Probes for detecting Nucleic acids-Cell Impermeant stains


83201 7-AAD [7-Aminoactinomycin D] 1 mgλ

abs: 546 nm; λ

em: 647 nm

83208 EthD-1 [Ethidium homodimer-1] 1 mgλ

abs: 528 nm; λ

em: 617 nm

83209 EthD-2 [Ethidium homodimer-2], 200 ml1 mM solution in DMSOλ

abs: 535 nm; λ

em: 624 nm

83222 Ethidium Bromide, 5 mM aqueous solution 10 xλ

abs: 518 nm; λ

em: 605 nm 1 mL

83221 Ethidium Bromide *UltraPure Grade* 10 xλ

abs: 518 nm; λ

em: 605 nm 100 mg

83214 Ethidium monoazide bromide 5 mgλ

abs: 462 nm; λ

em: 625 nm

83212 Propidium iodide 25 mgλ

abs: 535 nm; λ

em: 617 nm

83215 Propidium iodide, 1.0 mg/mL solution in water 10 mlλ

abs: 535 nm; λ

em: 617 nm

83013 Stains-All 100 mgλ

abs: 573 nm; λ

em: 609 nm


Labeling Nucleic acids/ FRET Probes

� Amino-modified Uridine derivatives for labelingNucleotides


83202 Aminoallyl dUTP 1 mg[5-(3-Aminoallyl)-2'’-deoxyuridine 5'’-triphosphate, trisodium salt]

83206 Aminoallyl dUTP, 4 mM in TE buffer 250 ml

83203 Aminoallyl dUTP, 4 mM in TE buffer 250 ml*UltraPure Grade*

83204 Aminoallyl dUTP *UltraPure Grade* 1 mg

83205 Aminoallyl dUTP *UltraPure Grade 1 mg* *Custom Packaging*

83207 Aminoallyl UTP 1 mg[5-(3-Aminoallyl)-uridine 5'’-triphosphate,trisodium salt]

60645 ARP [N-(Aminooxyacetyl)-N’’-(D- biotinoyl) 10 mghydrazine, trifluoroacetic acid salt]λ

abs: <300 nm; λ

em: none

83224 BrdU [5-Bromo-2’-deoxyuridine] 25 mgλ

abs: <300 nm; λ

em: none

83225 BrdUTP [5-Bromo-2’-deoxyuridine 100 uL5’-triphosphate], 10 mM in TE Bufferλ

abs: <300 nm; λ

em: none

83226 BrdUTP [5-Bromouridine 5’-triphosphate], 100 uL10 mM in TE Buffer λ

abs: <300 nm; λ

em: none

� HiLyte Fluor™ Dyes for Labelling Nucleic Acids


71238 HiLyte Fluor™ Amine-Reactive Dye, 0.1 mgHiLyte Fluor™ 488, SE *5-Pack*



� DABCYL based FRET Probes


23490 t-BOC-Lys(DABCYL)-OH 100 mgλ

abs: 428 nm; λ

em: none

Appl: A useful building block for FRETprobes containing DABCYL (as acceptor)

81800 DABCYL acid, [4-((4-(dimethylamino) phenyl)azo) 1 gbenzoic acid] *UltraPure Grade*λ

abs: 425 nm; λ

em: none


81801 DABCYL acid, SE, [4-((4- (dimethylamino)phenyl)azo) 100 mgbenzoic acid, succinimidyl ester]λ

abs: 453 nm; λ

em: none

Appl: It is the amino-reactiveform of DABCYL

81819 DABCYL C2 amine λabs:

428 nm; λem:

none 100 mgAppl: carbonyl-reactivebuilding block for developingDABCYL-based FRET probes.

81802 DABCYL C2 maleimide λabs:

428 nm; λem:

none 25 mgAppl: thiol-reactive buildingblock for developing DABCYL-basedFRET probes.

81820 DABCYL hydrazide λabs:

428 nm; λem:

none 100 mgAppl: carbonyl-reactivebuilding block for developing DABCYL-basedFRET probes.

81803 DABCYL Plus™ acid λabs:

437 nm; λem:

none 100 mgIt has much greater water solubilitythan DABCYL.

81804 DABCYL Plus™ acid, SE λabs:

454 nm; λem:

none 25 mgThe absorption spectrum of DABCYL Plus™is environment-sensitive

81823 DABCYL Plus™ C2 amine λabs:

430 nm; λem:

none 10 mgAppl: carbonyl-reactive buildingblock for developing DABCYL Plus™ -basedFRET probes.

81805 DABCYL Plus™ C2 maleimide λabs:

430 nm; λem:

none 10 mgAppl: thiol-reactive building blockfor developing DABCYL Plus™ -based FRET probes.

81824 DABCYL Plus™ hydrazide λabs:

430 nm; λem:

none 10 mgAppl: carbonyl-reactive buildingblock for developing DABCYL Plus™ -basedFRET probes.

81808 DABSYL-L-alanine λabs:

436 nm; λem:

none 25 mgAppl: reference standard for theHPLC analysis of L-alanine andalanine-containing peptides.

81806 DABSYL chloride *UltraPure Grade* 1 g[4-dimethylaminoazobenzene-4'- sulfonyl chloride]λ

abs: 436 nm (butylamine adduct); λ

em: none

Appl: acceptor for developingFRET-based nucleic acid probes andprotease substrates

81809 DABSYL-glycine λabs:

436 nm; λem:

none 25 mgAppl: standard for HPLC analysis ofglycine and glycin-containing peptides.

81807 DABSYL hydrazine λabs:

436 nm; λem:

none 100 mgAppl: building block that can be coupledto an aldehyde (for carbohydrates or glycoproteins)or carboxy group (for peptides or proteins).


FLUORESCENEFluorescent Probes/ FRET Probes


FRET Probes and QuenchersFLUORESCENCE

FRET Probes/Quenchers



81810 DABSYL-L-leucine 25 mgλ

abs: 436 nm; λ

em: none

reference standard for HPLC analysis of L-leucine and leucine-containing peptides.

81811 DABSYL-L-methionine 25 mgλ

abs: 436 nm; λ

em: none

reference standard for HPLCanalysis of L-methionine andmethionine-containing peptides.

81812 DABSYL-L-proline 25 mgλ

abs: 436 nm; λ

em: none

reference standard for HPLCanalysis of L-proline and proline-containingpeptides.

81813 DABSYL-L-tryptophan 25 mgλ

abs: 436 nm; λ

em: none

reference standard for HPLCanalysis of L-tryptophan and tryptophan-containing peptides.

81814 DABSYL-L-valine 25 mgλ

abs: 436 nm; λ

em: none

reference standard forHPLC analysis of L-valine and valine-containing peptides.

23496 FMOC-Lys(DABCYL)-OH, 100 mgλ

abs: 428 nm; λ

em: none

building block for FRETprobes containing DABCYL (as acceptor).

23858 FMOC-Lys(DABCYL) AnaResin™ 500 mgλ

abs: 428 nm; λ

em: none

building block for FRETprobes containing DABCYL (as acceptor)in solid-phase synthesis.


23486 t-BOC-Asp(EDANS)-OH 100 mgλ

abs: 341 nm; λ

em: 470

building block for FRETprobes containing EDANS

23488 t-BOC-Glu(EDANS)-OH 100 mgλ

abs: 341 nm; λ

em: 470


23887 EDANS acid, [5-((2-aminoethyl)amino) 1 gnaphthalene-1-sulfonic acid]λ

abs: 335 nm; λ

em: 493

81535 EDANS, sodium salt, [5-((2aminoethyl)amino)naphthalene-1-sulfonic acid,sodium salt] λ

abs: 335 nm; λ

em: 493

used for preparing 1 gFRET-based nucleic acid probes andprotease substrates.

23492 FMOC-Asp(EDANS)-OH 100 mgλ

abs: 341 nm; λ

em: 470


23494 FMOC-Glu(EDANS)-OH 100 mgλ

abs: 341 nm; λ

em: 470


� EDANS based FRET Probes

� Quenchers

DNP: An excellent amine-reactive FRET quencher paired with Trp or Tyr


81227 DNP-X acid, [6-(2,4-Dinitrophenyl) 100 mgaminohexanoic acid]λ

abs: ~350 nm; λ

em: none

81228 DNP-X acid, SE, [6-(2,4-Dinitrophenyl) 25 mgaminohexanoic acid, succinimidyl ester]λ

abs: ~350 nm; λ

em: none

81821 DNP C2 amine 100 mgλ

abs: ~350 nm; λ

em: none

81822 DNP C2 maleimide 25 mgλ

abs: ~350 nm; λ

em: none

� QXL:

QXL™ 490 dyes are the optimized quenchers for EDANS, AMCA and mostcoumarin fluorophores. QXL™ 490 acid can be coupled to amino groupsvia EDC-mediated reactions.

QXL™ 520 dyes are optimized quenchers for fluoresceins such as FAM,FITC, Rhodamine 6G and HiLyte Flour™ 488 flurophores.


81825 QXL™ 490 acid 100 mgλ

abs: 485 nm (in water); λ

em: none

81826 QXL™ 490 acid, SE 25 mgλ

abs: 495 nm(in water); λ

em: none

readily reacts withamino-containing compounds.

81828 QXL™ 490 C2 amine 10 mgλ


em: none

readily reactswith carbonyl-containing compounds.

81827 QXL™ 490 C2 maleimide 10 mgλ


em: none

readilyreacts with thiol-containing compounds.

81846 QXL™ 490 hydrazide 5 mgλ


em: none

readily reactswith carbonyl-containing compounds.

81830 QXL™ 520 acid 100 mgλ

abs: 508 and 530 nm; λ

em: none

can be coupledto amino groups via EDC-mediated reactions.



em: none

reacts with amino-containingcompounds.



em: none

readily reacts withcarbonyl-containing compounds.



em: none

readily reacts with thiol-containingcompounds.



em: none

readily reacts with carbonyl-containing compounds.


� QXL (Contd.)

QXL™ 570 dyes are optimized quenchers for rhodamines (such as TAMRA,sulforhodamine B, ROX) and Cy3 flurophores. It can be coupled to aminogroups via EDC-mediated reaction.

QXL™ 610 dyes are optimized quenchers for ROX and Texas Red®fluorophores. QXL™ 610 acid can be coupled to amino groups via EDC-mediated reactions.

QXL™ 670 dyes are optimized quenchers for Cy5 and Cy5-like fluorophoressuch as HiLyte Fluor™ 647. QXL™ 670 acid can be coupled to amino groupsvia EDC-mediated reactions.


81835 QXL™ 570 acid 25 mgλ

abs: 577 nm; λ

em: none


abs: 578 nm; λ

em: none readily reacts

with amino-containing compounds.


abs: 577 nm; λ

em: none



abs: 577 nm; λ

em: none

readily reacts with thiol-containing compounds.


abs: 578 nm; λ

em: none


81815 QXL™ 610 acid 100 mgλ

abs: 594 nm and 628 nm; λ

em: none



em: none

readily reacts with amino-containing compounds.



em: none




em: none


81817 QXL™ 610 vinyl sulfone *Thiol-Reactive* 25 mgλ


em: none

readily reacts with thiol-containing compounds.

81840 QXL™ 670 acid 10 mgλ

abs: 668 nm; λ

em: none


abs: 668 nm; λ

em: none

readily reacts with amino-containing compounds.


abs: 668 nm; λ

em: none readily reacts with

carbonyl-containing compounds.


abs: 668 nm; λ


thiol-containing compounds.


abs: 668 nm; λ



� QXL (Contd.)

QXL™ 680 dyes are optimized quenchers for Cy5 and Cy5-like fluorophoressuch as HiLyte Fluor™ 647. QXL™ 680 acid reacts with amine and hydroxyl-containing compounds through EDC-mediated reactions.


81855 QXL™ 680 acid 10 mgλ

abs: 679 nm; λ

em: none


abs: 679 nm; λ


with amino-containing compounds.


abs: 679 nm; λ




abs: 679 nm; λ


with thiol-containing compounds.


abs: 679 nm; λ



FRET Probes & QuenchersFLUORESCENCE

Fluorescent Probes/ FRET Probes


Quantification & Detection of Proteins on Gels/SolutionFLUORESCENCE

Protein detection

� AnaLyte™ OPA Protein Quantitation Kit*Fluorimetric*

AnaLyte™ OPA Protein Quantitation Kit is designed to quantify lowerconcentrations of proteins. This kit that employs the OPA reagent forrapid and sensitive protein quantitation in solution. The kit functionswell in the presence of lipids and detergents, the substances that inter-fere with many other protein determination methods.The kit is bestused to determine proteins in the range from 50 ng/mL to 25 mg/mL.


71015 AnaLyte™ OPA Protein Quantitation Kit 500*Fluorimetric* assays

� Reagents Used for Quantifying Peptides andProteins in Solution


81448 bBBr *UltraPure Grade* 25 mg[Dibromobimane]λ

abs: 393 nm; λ

em: 490 nm

81807 DABSYL hydrazide 100 mgλ

abs: 436 nm; λ

em: N/A nm

81520 Dansyl hydrazide 100 mg[5-Dimethylaminonaphthalene-1-sulfonyl hydrazine]λ

abs: 336 nm; λ

em: 534 nm

81202 Fluorescamine *UltraPure Grade* 25 mgλ

abs: 315 nm; λ

em: None

81433 mBBr *UltraPure Grade* 25 mg[Monobromobimane]λ

abs: 395 nm; Em(max): 490 nm

81203 NBD-Cl *UltraPure Grade* 100 mg[4-Chloro-7-nitrobenzofurazan]λ

abs: 337 nm; λ

em: None

81204 NBD-F *UltraPure Grade* 25 mg[4-Fluoro-7-nitrobenzofurazan]λ

abs: 337 nm; λ

em: None

83010 NDA *UltraPure Grade* 100 mg[Naphthalene-2,3-dicarboxaldehyde]λ

abs: 4194 nm; λ

em: 4934 nm

83012 OPA *UltraPure Grade* 1 g[o-Phthaldialdehyde]λ

abs: 3345 nm; λ

em: 4565 nm

81408 SBF-Cl 25 mg[4-Chloro-7-sulfobenzofurazan,ammonium salt]λ

abs: 380 nm; λ

em: None

81409 SBF-F 10 mg[4-Fluoro-7-sulfobenzofurazan,ammonium salt]λ

abs: 385 nm; λ

em: None


83004 Alcian Blue 8GX *UltraPure Grade* 100 mgλ

abs: 615 nm; λ

em: N/D nm

83005 Alcian Blue 8GX Plus*UltraPure Grade* 100 mgλ

abs: 670 nm; λ

em: N/D nm

81513 2-Aminoacridone 25 mgλ

abs: 425 nm; λ

em: 531 nm

81529 ANDS [7-Aminonaphthalene-1,3- 1 gdisulfonic acid, potassium salt]λ

abs: 350 nm; λ

em: 450 nm

81524 2,6-ANS [2-Anilinonaphthalene- 100 mg6-sulfonic acid]λ

abs: 319 nm; λ

em: 422 nm

81525 1,8-ANS [1-Anilinonaphthalene- 100 mg8-sulfonic acid]λ

abs: 319 nm; λ

em: 422 nm

81528 ANTS [8-Aminonaphthalene-1,3, 100 mg6-trisulfonic acid,disodium salt] *UltraPure Grade*λ

abs: 353 nm; λ

em: 520 nm

81523 APTS [8-Aminopyrene-1,3,6-trisulfonic 10 mgacid, trisodium salt]λ

abs: 424 nm; λ

em: 505 nm

60650 Biotin hydrazide 25 mg

60652 Biotin-XX hydrazide 25 mg[6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid, hydrazide]

83016 Congo Red *UltraPure Grade* 1 gλ

abs: 497 nm; λ

em: N/A nm

83002 Coomassie® Brilliant Blue G-250 100 mg*UltraPure Grade*λ

abs: 610 nm; λ

em: N/A nm

83003 Coomassie® Brilliant Blue R-250 1 g*UltraPure Grade*λ

abs: 585 nm; λ

em: N/A nm

81807 DABSYL hydrazide 100 mgλ

abs: 436 nm; λ

em: N/A nm

81520 Dansyl hydrazide 100 mg[5-Dimethylaminonaphthalene-1-sulfonyl hydrazine] λ

abs: 336 nm;

λem

: 534 nm

83017 Eosin Y *UltraPure Grade* 1 gAbs(max): 517 nm; λ

em: N/D nm

83007 Fast Green FCF *UltraPure Grade* 1 gλ

abs: 622 nm; λ

em: N/A nm

81505 5-FTSC [Fluorescein-5-thiosemicarbazide] 25 mgλ

abs: 492 nm; λ

em: 516 nm

83011 Nile Red 25 mgλ

abs: 552 nm; λ

em: 636 nm

83000 10% Ninhydrin in ethanol 10 mlλ

abs: N/D nm; λ

em: N/D nm

83008 Oil Red O *UltraPure Grade* 1 gλ

abs: 518 nm; λ

em: N/A nm

83009 Ponceau S *UltraPure Grade* 1 gλ

abs: 520 nm; λ

em: N/A nm


abs: 573 nm; λ

em: 609 nm

� Detecting Proteins on Gels


� AnaTag™ Protein Labeling Kits

� AnaTag™ AMCA-X Protein Labeling Kit

This kit is optimized to conjugate AMCA-X to proteins (e.g., IgG). Oneconjugation reaction can label up to 5 mg protein.

� AnaTag™ 5-FAM Protein Labeling Kit

This kit is optimized to conjugate 5-FAM (5-Carboxyfluorescein) to pro-teins (e.g., IgG). One conjugation reaction can label up to 5 mg protein.

� AnaTag™ 5-TAMRA Protein Labeling Kit

This kit is optimized to conjugate 5-TAMRA (5-Carboxytetramethylrhodamine) to proteins (e.g., IgG). One conjugationreaction can label up to 5 mg protein.

� AnaTag™ 5-ROX Protein Labeling Kit

This kit is optimized to conjugate 5-ROX (5-Carboxy-X-rhodamine) toproteins (e.g., IgG). One conjugation reaction can label up to 5 mgprotein.

� AnaTag™ TR Protein Labeling Kit

This kit is optimized to conjugate HiLyte Fluor™ TR to proteins (e.g.,IgG). One conjugation reaction can label up to 1 mg protein.

� AnaTag™ HiLyte Fluor 555 Protein Labeling Kit

This kit is optimized to conjugate HiLyte Fluor™ 555 SE to proteins (e.g.,IgG). One conjugation reaction can label 100 µg of protein.

� AnaTag™ HiLyte Fluor 647 Protein Labeling Kit

This kit is optimized to conjugate HiLyte Fluor™ 647 SE to proteins (e.g.,IgG). One conjugation reaction can label 100 µg of protein.

� AnaTag™ Biotin Protein Labeling Kit

This kit is optimized to conjugate biotin-X, SE (d-Biotin-amidocaproate-N-hydroxysuccinimide ester) to proteins (e.g., IgG).One reaction can label up to 10 mg IgG.

� AnaTag™ AP Protein Labeling Kit

The AnaTagTM Alkaline Phosphatase Protein Labeling Kit is optimized forlabeling proteins with alkaline phosphatase. This kit provides ample ma-terials to label and desalt up to 1 mg of Ig G.

� AnaTag™ HRP Protein Labeling Kit

This kit provides an ultra convenient method to conjugate HRP to pro-tein. It contains formulated and preactivated HRP, stabilizer and allnecessary reagents/buffer to prepare one reaction of HRP-protein con-jugation.


71000 AnaTag™ AMCA-X Protein Labeling Kit 5 Rxns.

71001 AnaTag™ 5-FAM Protein Labeling Kit 5 Rxns.

71002 AnaTag™ 5-TAMRA Protein Labeling Kit 5 Rxns.

71005 AnaTag™ 5-ROX Protein Labeling Kit 5 Rxns.

71006 AnaTag™ TR Protein Labeling Kit 5 Rxns.

71007 AnaTag™ HiLyte Fluor 555 Protein Labeling Kit 5 Rxns.

71008 AnaTag™ HiLyte Fluor 647 Protein Labeling Kit 5 Rxns.

71003 AnaTag™ Biotin Protein Labeling Kit 5 Rxns.

71009 AnaTag™ AP Protein Labeling Kit 1 kit

71010 AnaTag™ HRP Protein Labeling Kit 1 kit

� AnaTag™ APC Protein Labeling Kit

The EnzoLyte™ APC protein labeling kit is optimized for labeling APC-XL(a stable, chemically cross-linked APC trimer) to antibodies and otherproteins. APC (Allophycocyanin) is an ultra-sensitive fluorescent tracerbecause of its high emission quantum yields. Its fluorescence can bedetected at the emission wavelength of 660±5 nm or at 650±5 nm whenexcited.


71011 AnaTag™ APC Protein Labeling Kit 1 kit

� AnaTag™ BPE Protein Labeling Kit

The EnzoLyte™ BPE protein labeling kit is optimized for labeling BPE toantibodies and other proteins. B-PE (B-Phycoerythrin) is a fluorescentprotein that has absorption bands with peaks at 545 nm and 563 nm andhas maximal emission at 578 nm. B-PE labeled biotin, avidin, and primaryand secondary antibodies have been widely applied to flow cytometry,live cell staining, and multi-color immunofluorescent staining.


71012 AnaTag™ BPE Protein Labeling Kit 1 kit

� AnaTag™ RPE Protein Labeling Kit

The EnzoLyte™ RPE protein-labeling kit is optimized for labeling RPE toantibodies and other proteins. R-PE (R-Phycoerythrin), a fluorescentprotein, belongs to the phycobiliproteins family. It has broad absorptionbands with peaks at 565 nm, 498 nm, and 539 nm, therefore can beexcited with versatile excitation sources. Its maximal emission can bedetected at 578 nm. R-PE labeled biotin, avidin, primary and secondaryantibody have been widely applied to flow cytometry, live cell staining,and multi-color immunofluorescent staining.


71013 AnaTag™ RPE Protein Labeling Kit 1 kit1 Reaction (1x1 mg protein)

� AnaLyte™ CB Protein Quantitation Kit*Colorimetric*

AnaLyte™ CB Protein Quantitation Kit is a modification of Bradfordmethod. This kit is designed to quantify concentrations of proteins higherthan 1,500 µg/mL. Sufficient reagents are provided to perform 1000assays, based on a 200 µL assay volume in a 96-well microplate format.The assay can also be adapted for use in cuvettes. The assay is based onthe immediate absorption shift from 465 nm to 595 nm when Coomassie®Blue G-250 binds to proteins in acidic solutions. The kit has been speciallyformulated with ready-to-use buffers, prediluted standards. Commoncontaminants, including salts, solvents, mercaptoethanol, amino acidsand DNA, are well tolerated in this assay. However, it is not compatiblewith detergents.


71014 AnaLyte™ CB Protein Quantitation Kit 500*Colorimetric* assays

Kits for Labeling Proteins with dyes & enzymesFLUORESCENCE

Labeling & Detection of Proteins


Calcium Detection ReagentsFLUORESCENCE

Calcium Detection

� UV- Excitable Calcium Indicators

� Fura-2

λabs

: 363 (335) nm; λem

: 512 (505) nm


84015 Fura-2, AM 1 mg

84016 Fura-2, AM *UltraPure Grade* 1 mg

84017 Fura-2, AM *UltraPure Grade* 1 mgCustom Packaging

84018 Fura-2, AM *UltraPure Grade*, 1 mM 1 mlsolution in anhydrous DMSO

84019 Fura-2, AM *UltraPure Grade* Bulk Packaging 50 mg

84011 Fura-2, pentapotassium salt 1 mg

84012 Fura-2, pentasodium salt 1 mg

� Indo-1λ

abs: 346 (330) nm; λ

em: 475 (401) nm


84006 Indo-1, AM 1 mg

84007 Indo-1, AM *UltraPure Grade* 1 mg

84008 Indo-1, AM *UltraPure Grade* Custompackaging 1 mg

84009 Indo-1, AM *UltraPure Grade*, 1 mM solutionin anhydrous DMSO 1 mg

84010 Indo-1, AM *UltraPure Grade* 50 mg*Bulk packaging*

84000 Indo-1, pentapotassium salt 1 mg

84001 Indo-1, pentasodium salt 1 mg

� Indo-5Fλ

abs: 347 (331) nm; λ

em: 475 (412) nm


84050 Indo-5F, pentapotassium salt 1 mg

84051 Indo-5F, AM 1 mg

� Visible Light-Excitable Calcium Indicators

� Calceinλ

abs: 494 nm; λ

em: 517 nm


89200 Calcein *UltraPure Grade* 100 mg

89201 Calcein, AM 1 mg

89202 Calcein, AM *UltraPure Grade* 1 mg

89203 Calcein, AM *UltraPure Grade*, 5 mM 200 µLsolution in anhydrous DMSO

89204 Calcein, AM *UltraPure Grade* 1 mg*Custom Packaging*

89206 Calcein blue 250 mgAbs (max): 322 nm; Em (max): 437 nm

89205 Calcein blue, AM 25 mgAbs (max): 494 nm; Em (max): 517 nm

� Dihydrocalcein

λabs

: 494 nm; λem

: 517 nm

Reduced form of calcein AM


85700 Dihydrocalcein, AM 1 mg

85701 Dihydrocalcein, AM *Custom Packaging* 1 mg

� Fluo-3

λabs

: 506 nm; λem

: 526 nm

The most popular cell-permeable calcium indicator for functional GPCRassays


84024 Fluo-3, AM 1 mg

84025 Fluo-3, AM *UltraPure Grade* 1 mg

84026 Fluo-3, AM *UltraPure Grade* CustomPackaging 1 mg

84027 Fluo-3, AM *UltraPure Grade*, 1 mM solutionin anhydrous DMSO 1 mL

84028 Fluo-3, AM *UltraPure Grade* *Bulk Package* 50 mgFluorescence enhancement upon binding Ca2+,Mg2+, Ba2+, Cd2+, Hg2+, Pb2+, Zn2+ and La3+

84020 Fluo-3, pentaammonium salt 1 mg

84021 Fluo-3, pentapotassium salt 1 mg

84022 Fluo-3, pentasodium salt 1 mg

� Fluo-5F

λabs

: 494 nm; λem

: 518 nm


84055 Fluo-5F, pentapotassium salt 1 mgSensitive fluorescent Ca2+ indicator,Kd = 2.3 mM

84056 Fluo-5F, AMCell-permeable fluorescent Ca2+ indicator 1 mg

� Fluo-5N

λabs

: 593 nm; λem

: 518 nm


84057 Fluo-5N, pentapotassium salt 1 mgFluorescence enhancement upon bindingCa2+, Cd2+, Hg2+ and La3+

84058 Fluo-5N, AM 1 mgLow affinity cell-permeable Ca2+ indicator

� Visible Light-Excitable Calcium Indicators

� X-Rhod-1

λabs

: 580 nm; λem

: 602 nm


84049 X-Rhod-1, tripotassium salt 1 mg

84048 X-Rhod-1, AM 1 mg


Calcium Detection Reagents

� Rhod-2λ

abs: 549 nm; λ

em: 578 nm

Long wavelength fluorescent indicator for quantifying intracellular Ca2+concentration


84034 Rhod-2, AM 1 mg

84035 Rhod-2, AM *UltraPure Grade* 1 mg

84036 Rhod-2, AM *UltraPure Grade* Custom Pack 1 mg

84037 Rhod-2, AM *UltraPure Grade*,1 mM solution in anhydrous DMSO 1 mg

84038 Rhod-2, AM *UltraPure Grade* Bulk Pack 50 mg

84030 Rhod-2, tripotassium saltFluorescence enhancement upon bindingCa2+, Mg2+, Ba2+, Cd2+, Hg2+, Pb2+,Zn2+ and La3+ 1 mg

84031 Rhod-2, trisodium saltFluorescence enhancement upon binding Ca2+,Mg2+, Ba2+, Cd2+, Hg2+, Pb2+, Zn2+ and La3+ 1 mg

� Rhod-5Fλ

abs: 580 nm; λ

em: 602 nm

Long wavelength fluorescent Ca2+ indicator


84059 Rhod-5F, tripotassium salt 1 mg

84060 Rhod-5F, AM 1 mg

� Rhod-5Nλ

abs: 551 nm; λ

em: 577 nm


84061 Rhod-5N, tripotassium salt 1 mg

84062 Rhod-5N, AM 1 mg

� Coelenterazine Sampler Kit *UltraPure Grade*

This Coelenterazine Sampler Kit consists of coelenterazine and four ofits derivatives, 25 µg each, for reconstituting aequorin in cells that havebeen transfected with apoaequorin DNA. The coelenterazine analogs con-fer different Ca2+ affinities and spectral properties on he aequorincomplex.


71403 Coelenterazine Sampler Kit *UltraPure Grade* 1 kit

� Coelenterazine and Its Synthetic Analogs forLuminescent Calcium Detection

The aequorin complex comprises a 22,000-dalton apoaequorin protein,molecular oxygen and the luminophore coelenterazine. When three Ca2+ions bind to this complex, coelenterazine is oxidized to coelenteramide,with a concomitant release of carbon dioxide and blue light. It has abroad detection range from ~0.1 µM to >100 µM. Aequorins containingthe cp, f or h form of coelenterazine exhibit 10–20 times stronger lumi-nescence than that of apoaequorin reconstituted with nativecoelenterazine. Coelenterazine cp has been used in HTS screening assayfor GPCRs.


82255 Coelenterazine *UltraPure Grade* 0.25 mgAbs (max): 429 nm; Em (max): 466 nm

82256 Coelenterazine cp *UltraPure Grade* 0.25 mgAbs (max): 430 nm; Em (max): 442 nm

82257 Coelenterazine f *UltraPure Grade* 0.25 mgAbs (max): 437 nm; Em (max): 472 nm

82258 Coelenterazine h *UltraPure Grade* 0.25 mgAbs (max): 437 nm; Em (max): 466 nm

82259 Coelenterazine hcp *UltraPure Grade* 0.25 mgAbs (max): 437 nm; Em (max): 445 nm

82260 Coelenterazine n *UltraPure Grade* 0.25 mgAbs (max): 431 nm; Em (max): 468 nm

� Non- Luminescent Calcium Indicators


84070 BAPTA, AM # 25 mg

84071 BAPTA, AM *UltraPure Grade* # 25 mg

84072 BAPTA, tetrapotassium salt ## 100 mg

84073 BAPTA, tetrasodium salt ## 100 mg

84074 5,5’-Difluoro BAPTA, AM # 25 mg

84075 5,5’-Difluoro BAPTA, tetrapotassium salt ## 100 mg

84076 5,5’-Dimethyl BAPTA, AM # 25 mg

84077 5,5’-Dimethyl BAPTA, tetrapotassium salt ## 100 mg

84080 5,5’-Dinitro BAPTA, AM # 25 mg

84081 5,5’-Dinitro BAPTA, free acid ## 100 mg

84100 EGTA, AM # 10 mg

84097 EGTA, tetrasodium salt, 10 mM aqueous 10 mLsolution *UltraPure Grade*A chelating agent for the determinationof calcium in the presence of magnesium

84040 Pluronic® F-127 *Cell Culture Tested * 10 gCell culture reagent for dissolving AM esters

84041 Pluronic® F-127, 20% solution in DMSO 10 mLCell culture reagent for dissolving AM esters

84042 Pluronic® F-127, 10% solution in water 100 mLCell culture reagent for dissolving AM esters

# Cell permeable

## Cell impermeable

FLUORESCENCECalcium Detection


Fluorecent substrates for Esterase/Oxidase for Cell ViabilityFLUORESCENCE

Cell viability, proliferation & toxicity

� Fluorogenic Esterase and Oxidase SubstratesUsed for Cell Viability Assays


84602 BCECF, AM, 1 mg/mL solution in anhydrous 1 mlDMSOλ

abs: 503 nm; λ

em : 520 nm,

84603 BCECF, AM *Custom Packaging* 1 mgProperties same as above

85703 5-(and-6)-Carboxy-2'’,7'’- 25 mgdichlorodihydrofluorescein diacetateλ

abs: 495 nm; λ

em : 529 nm)

Cell-impermeable substrate fordetection of oxidases (including peroxidase)

85702 5-Carboxy-2'’,7'’-dichlorodihydrofluorescein 5 mgdiacetate, di(acetoxymethyl ester)λ

abs: 495 nm; λ

em : 529 nm

Cell-permeable substrate for fluorogenicdetection of oxidases (including peroxidase)

89003 5-(and-6)-Carboxy-2'’,7'’- 100 mgdichlorofluoresceindiacetate *Mixed Isomers*λ

abs: 495 nm; λ

em : 529 nm

pH indicator for acidic pH range

89005 5(6)-CFDA [5-(and-6)-Carboxyfluorescein 100 mgdiacetate] *Mixed Isomers*λ

abs: 492 nm; λ

em : 517 nm,

pH indicator for slightly acidic pH range

89007 5-CFDA [5-Carboxyfluorescein diacetate] 100 mg*Single Isomers* λ

abs : 492 nm;

λem

: 517 nm)pH indicator for slightlyacidic pH range

89008 6-CFDA [6-Carboxyfluorescein diacetate] 100 mg*Single Isomers* λ

abs: 492 nm;

λem

: 517 nm)pH indicator for slightlyacidic pH range

89009 5-CFDA, AM [5-Carboxyfluorescein diacetate, 5 mgacetoxymethyl ester]λ

abs : 492 nm; λ

em : 517 nm

Cell-permeable pH indicatorfor slightly acidic pH range

85706 2'’,7'’-Dichlorodihydrofluorescein diacetate 100 mg[2'’,7'’-Dichlorofluorescin diacetate]λ

abs: 495 nm; λ

em : 529 nm

Cell-permeable substrate fordetection of oxidases (including peroxidase)

85700 Dihydrocalcein, AM 1 mgλ

abs: 494 nm; λ

em : 517 nm

Generic cell permeable substrate fordetection of oxidases(including peroxidase);is well retained in cells.

85701 Dihydrocalcein, AM *Custom Packaging* 1 mgλ

abs: 494 nm; λ

em : 517 nm

Also available Calcein & Calcein Blue. Please view page #

� Fluorescent Vital Stains for MonitoringCellular Functions


84737 DBDS [4,4'’-Dibenzamidostilbene-2,2'’- 100 mgdisulfonic acid, disodium salt]λ

abs : 343 nm; λ

em: 430 nm

Environment-sensitive dye for studyingmembrane anion transporter

84915 DNDS [4,4'’-Dinitrostilbene-2,2'’- 1 gdisulfonic acid, disodium salt]λ

abs : 352 nm; λ

em: N/A

Fluorescent probe for studying iontransporter

84710 DiA [4-(4-(Dihexadecylamino) styryl)-N- 25 mgmethylpyridinium iodide]λ

abs : 491 nm; λ

em: 613 nm

Fluorescent lipophilic tracer

� Fluorogenic Esterase and Oxidase SubstratesUsed for Cell Viability Assays (Contd.)


85701 Dihydrocalcein, AM 1 mgλ

abs: 494 nm; λ

em 517 nm *

Reduced form of calcein AM

85717 Dihydroethidium 10 mg*Special Air-free Packaging*λ

abs: 518 nm; λ

em: 605 nm

Generic substrate for detectionof oxidases (including peroxidase); Theoxidized product tends to accumulate in nuclei.

85718 Dihydroethidium 1 mL/5 mM solution in DMSO 10 mLλ

abs: 355 nm; λ

em: N/A Bind to DNA/RNA

(red fluorescence) upon oxidation

85710 Dihydrofluorescein diacetate 25 mgλ

abs: 492 nm; λ

em: 517 nm

Generic substrate for detectionof oxidases (including peroxidase)

85711 Dihydrorhodamine 123 10 mgλ

abs : 507 nm; λ

em: 529 nm

Generic substrate for detection of oxidases(including peroxidase) in mitochondria

85720 Dihydrorhodamine 123 *Air-free Packaging* 10 mgProperties same as above

85719 Dihydrorhodamine 123, 5 mM solution in 1 mLDMSO Properties same as above

85712 Dihydrorhodamine 6G 25 mgλ

abs: 528 nm; λ

em: 551 nm

Generic substrate for detectionof oxidases (including peroxidase)in mitochondria

88400 FDA [Fluorescein diacetate] 1 gλ

abs: 490 nm; λ

em: 514* nm,

Cell-permeable pH indicator forslightly acidic pH range


� Fluorescent Vital Stains for Monitoring CellularFunctions (Contd.)


84700 DiBAC4(3) *UltraPure Grade* 25 mg[Bis-(1,3-dibutylbarbituric acid)trimethine oxonol]λ

abs: 493 nm; λ

em: 516 nm

Sensitive 488 nm-excitable membranepotential probe,~1% fluorescence intensity change/mV;

84701 DiBAC4(5) *UltraPure Grade* 25 mg[Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]λ

abs: 590 nm; λ

em: 616 nmv

Sensitive membrane potential probewith longer wavelength

84903 DiD [1,1'’-Dioctadecyl-3,3,3'’,3'’ 25 mgtetramethylindodicarbocyanineperchlorate] *Oil*λ

abs: 644 nm; λ

em: 665 nm

Lipophilic membrane tracer

84920 DiD [1,1'’-Dioctadecyl-3,3,3'’,3'’ 10 mgtetramethylindodicarbocyanine,4-chlorobenzenesulfonate salt] *Solid*Properties same as above

84904 DIDS [4,4'’-Diisothiocyanatostilbene-2,2'’- 100 mgdisulfonic acid, disodium salt]λ

abs: 341 nm; λ

em: 415 nm

Reactive environment-sensitive dye forstudying receptors and proteins

84711 DiI [1,1'’-Dioctadecyl-3,3,3'’,3'’- 100 mgtetramethylindocarbocyanine perchlorate]λ

abs: 549 nm; λ

em: 565 nm

A lipophilic membrane stain that diffuseslaterally to stain the entire cell; Its

84906 D9-DiI [1,1'’-Dioleyl-3,3,3'’,3'’- 25 mgtetramethylindocarbocyaninemethanesulfonate]λ

abs: 549 nm; λ

em: 564 nm

A lipophilic membrane stain

84709 DiIC1(5) 100 mg[1,1'’,3,3,3'’,3'’-Hexamethylindodicarbocyanine iodide]λ

abs: 638 nm; λ

em: 658 nm

Used for measurement of mitochondrialmembrane potential

84902 DiIC12(3) 100 mg[1,1'’-Didodecyl-3,3,3'’,3'’-tetramethylindocarbocyanine perchlorate]λ

abs: 549 nm; λ

em: 565 nm

Lipophilic neuronal tracer

84905 DiIC16(3) [1,1 100 mgλ

abs: 549 nm; λ

em: 565 nm

Lipophilic neuronal tracerss

84907 DiIC18(3)-DS [1,1'’-Dioctadecyl-3,3,3'’,3'’ 5 mgtetramethylindocarbocyanine-5,5'’-disulfonic acid]λ

abs: 555 nm; λ

em: 570 nm




84908 DiIC18(5)-DS 5 mg[1,1'’-Dioctadecyl-3,3,3'’,3'’tetramethylindodicarbocyanine-5,5'’-disulfonic acid] λ

abs: 650 nm;

λem

: 670 nmLipophilic neuronal tracer

84712 DiO [3,3'’-Dioctadecyloxacarbocyanine 100 mgperchlorate λ

abs: 484 nm; λ

em:

501 nm Lipophilic neuronal tracer

84706 DiOC2(3) [3,3'’-Diethyloxacarbocyanine 100 mgiodide] λ

abs: 482 nm; λ

em: 497 nm

Membrane potential-dependentred-shifted fluorescence

84714 DiOC5(3) [3,3'’-Dipentyloxacarbocyanine 100 mgiodide] λ

abs: 484 nm; λ

em: 500 nm

measuring membrane potential

84715 DiOC6(3) [3,3'’-Dihexyloxacarbocyanine 100 mgiodide] λ

abs: 484 nm; λ

em: 501 nm

Most widely used for measuring membranepotential

84707 DiOC7(3) [3,3'’-Diheptyloxacarbocyanine 100 mgiodide] λ

abs: 482 nm; λ

em: 504 nm

Used for measuring membrane potentialof mitochondria

84708 DiOC16(3) 25 mg[3,3'’-Dihexadecyloxacarbocyanineperchlorate] λ

abs: 484 nm; λ

em: 501 nm

Lipophilic membrane tracer

84916 1,1'’-Dioctadecyl-5,5'’-diphenyl-3,3,3'’, 3'’ 5 mgtetramethylindocarbocyanine chlorideλ

abs: 576 nm; λ

em: 599 nm


84922 DiR [1,1'’-Dioctadecyl-3,3,3'’,3'’ 10 mgtetramethylindotricarbocyanine iodidevλ

abs: 748 nm; λ

em:780 nmv


84702 DiSBAC2(3) 25 mg/[Bis-(1,3-diethylthiobarbituric acid) 100 mgtrimethine oxonol]λ

abs: 535 nm; λ

em: 560 nm

Sensitive membrane potential probe,less temperature-dependent than DiBAC

84923 DiSC3(5) 100 mg[3,3'’-Dipropylthiadicarbocyanine iodide]λ

abs: 651 nm; λ

em: 675 nm

Accumulate in cells on hyperpolarizedmembranes

88200 5-Dodecanoylaminofluorescein 100 mgλ

abs: 495 nm; λ

em: 518 nm

Cell membrane stain

88202 DPH [1,6-Diphenyl-1,3,5-hexatriene] 25 mgλ

abs:350 nm; λ

em: 452 nm

Environment-sensitive dye for studyingmembrane and protein structures

Fluorescent Stains for Cell ViabilityFLUORESCENCE



Fluorecent Stains for Cell Viability/Nucleic acid stainsFLUORESCENCE




88203 DPH propionic acid 25 mg[3-(4-(6-Phenyl)-1,3,5-hexatrienyl)phenylpropionic acid] λ

abs: 354 nm;

λem

: 430 nm Cell membrane stain

88060 JC-1 [5,5'’,6,6'’-tetrachloro-1,1'’,3,3'’- 5 mgtetraethylbenzimidazolylcarbocyanine iodide]λ

abs: 514 nm; λ

em: 529 nm

Widely used for measuring membranepotential of mitochondria; 585/520Fluorescence ratio increases upon cellhyper-polarization.

88201 Laurdan 25 mg[6-Dodecanoyl-2-dimethylaminonaphthalene]λ

abs: 364 nm; λ

em: 497 nm

Environment-sensitive dye for studyingmembrane and protein structures

84720 Merocyanine 540 25 mgλ

abs: 555 nm; λ

em: 578 nm

The first fluorescent dye used formeasuring membrane potential;phototoxic agent

88210 5-Octadecanoylaminofluorescein 100 mgλ

abs: 497 nm; λ

em: 519 nm

Cell membrane stain; used for staininglatex and liposome

84703 Oxonol V 100 mg[Bis-(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol] λ

abs: 610 nm;

λem

: 639 nm Fluorescence decreaseupon membrane hyperpolarization

84704 Oxonol VI 100 mg[Bis-(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol] λ

abs: 599 nm;

λem

: 634 nm

88212 Prodan 25 mg[6-Propionyl-2-dimethylaminonaphthalene]α

abs: 363 nm; Em (max): 497 nm

Environment-sensitive dye for studyingmembranes and structures of proteins

88310 1,3,6,8-Pyrenetetrasulfonic acid, 100 mgtetrasodium salt λ

abs: 374 nm; λ

em: 403 nm

Hydrophilic cell tracer

88054 Rhodamine 123 *UltraPure Grade* 25 mgλ

abs: 507 nm; λ

em: 529 nm

Widely used for measuring membranepotential of mitochondria; Fluorescence isless dependent on dye location

88058 Rhodamine B, hexyl ester, perchlorate 10 mgλ

abs: 556 nm; λ

em: 578 nm

Mitochondrial stain

88316 SITS [4-Acetamido-4'’- 100 mgisothiocyanatostilbene- 2,2'’- disulfonic acid,disodium salt] λ

abs: 336 nm; λ

em: 436 nm

Fluorescent probe for studying ion transporter

84918 SP-DiIC18(3) [1,1'’-Dioctadecyl-6,6'’- 5 mgdi(4-sulfophenyl) -3,3,3'’,3'’tetramethylindocarbocyanine]λ

abs: 556 nm; λ

em: 573 nm



84917 SP-DiOC18(3) [3,3'’-Dioctadecyl-5,5'’- 5 mgdi(4-sulfophenyl) oxacarbocyanine,sodium salt]λ

abs: 497 nm; λ

em: 513 nm


84732 SPEEDY™ DiA 5 mg[4-(4-(Dilinoleylamino)styryl)-N-methylpyridinium iodide] *Oil*λ

abs: 492 nm; λ

em: 612 nm

Fluorescent lipophilic tracer

88061 TMRE 25 mg[Tetramethylrhodamine, ethyl ester,perchlorate]λ

abs: 549 nm; λ

em: 574 nm

Used for measuring membrane potentialof mitochondria; Fluorescence is lessdependent on dye location.

88065 TMRM [Tetramethylrhodamine, 25 mgmethyl ester, perchlorate)]λ

abs: 549 nm;λ

em: 573 nm

Used for measuring membrane potentialof mitochondria; Fluorescence is lessdependent on dye location.

� Cell-Impermeant Nucleic Acid Stains Used for CellViability Assays


83208 EthD-1 [Ethidium homodimer-1] 1 mgλ

abs: 528 nm; λ

em: 617 nm

Higher affinity to nucleic acids than EtBr

83209 EthD-2 [Ethidium homodimer-2], 200 ml1 mM solution in DMSOλ

abs: 535 nm; λ

em: 624 nm

Higher affinity to nucleic acids than EtBr

83222 Ethidium Bromide, 5 mM aqueous solution 10 xλ

abs: 518 nm; λ

em: 605 nm 1 mL

83221 Ethidium Bromide *UltraPure Grade* 1 gproperties same as above

83214 Ethidium monoazide bromide 5 mgλ

abs: 462 nm; λ

em: 625 nm

A fluorescent photoaffinity label that couplescovatently to nucleic acids upon light irradiation

83212 Propidium iodide 25 mgλ

abs: 535 nm; λ

em: 617 nm

83215 Propidium iodide, 1.0 mg/mL solution in water 10 mlproperties same as above


abs: 573 nm; λ

em: 609 nm

Differential staining of nucleic acidsand proteins; RNA (bluish purple),DNA (blue) and proteins (red)


LDH based Assay Kits for Analyzing Cell Viability & ProliferationFLUORESCENCE

Assay kits for Cell Viability/Cytotoxicity

� DHL™ Cell Viability and Proliferation Assay Kit*Fluorimetric*

Lactate dehydrogenase (LDH) generally exists in the cytoplasm of livingmammalian cells. The measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viability.The kit provides researchers a one-step assay to count living cells in aculture and continuously monitor cell proliferation over time by measur-ing cytoplasmic LDH activity. The kit uses resazurin as a sensitivefluorogenic indicator, which is converted to resozufin (excitation/emission=560nm /590 nm) by cytoplasmic LDH3-5. It can detect as littleas 48 living cells with the linear range up to 5X104 cells (r2=0.99). It issuitable for high throughput screening of cell proliferation or cytotoxic-ity effect of a variety of compounds.


71300 DHL™ Cell Viability and Proliferation 2000Assay Kit *Fluorimetric* assays

71301 DHL™ Cell Viability and Proliferation 10,000Assay Kit *Fluorimetric* assays

� DHL™ Express Cell Counting Kit *Fluorimetric*

DHL™ Express Cell Counting Kit provides a convenient 15-minute assay toquantify the total cell number (including live cells and dead cells) bymeasuring total LDH activity in cytoplasm and culture medium. Upon celldeath, LDH is released into surrounding medium. The kit uses resazurin,a sensitive indicator for cell viability. Resazurin is converted to resozufin(excitation/emission=560 nm/590 nm) by LDH with a coupled enzymaticreaction. The kit can detect as little as 97 cells with the linear range upto 2.5X104 cells (r2=0.95). It is suitable for high throughput screening ofcell proliferation or cytotoxicity effect of a variety of compounds. Thekit contains: Assay mixture, Assay buffer, Lysis solution, Stop solution, Adetailed protocol


71405 DHL™ Express Cell Counting Kit 500*Fluorimetric* assays

71406 DHL™ Express Cell Counting Kit 5000*Fluorimetric* assays

� DHL™ Cell Cytotoxicity Assay Kit *Fluorimetric*

The damage of cell membrane leads to the release of cytoplasmic enzymes.The measurement of released cytoplasmic lactate dehydrogenase (LDH)is a well-accepted assay to estimate cell membrane integrity and quantifycytotoxicity. The EnzoLyte™ Cell Cytotoxicity Assay Kit uses resazurin asa sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon converted)to measure LDH activity. The assay can be performed in a mixed populationof damaged and viable cells, but it only measures the LDH released fromdamaged cells. The cytoplasmic LDH in living cells produces little signalsunder assay condition. The fluorescent signal is proportional to the numberof damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limitreaching 100 dead cells.

The kit contains: • Assay mixture• Assay buffer• Lysis solution• Stopsolution• A detailed protocol


71302 DHL™ Cell Cytotoxicity Assay Kit 500*Fluorimetric* assays

71303 DHL™ Cell Cytotoxicity Assay Kit 5000*Fluorimetric* assays


FISH PaintsCYTOGENETICS

StarFish chromosomal Paints

� STAR*FISH™ Chromosome Painting

The Cambio STARHFISH Chromosome Painting System has been devel-oped in collaboration with a leading molecular cytogenetics laboratory inthe University of Cambridge, U.K. The products come with Cambio’sguarantee of quality, and are backed with the full technical support ofthe Cambridge University molecular cytogenetics laboratory. Cambio’sChromosome Paints are uniquely prepared from PCR* amplified DNAobtained from FACS-sorted chromosomes. F. Hoffmann-La Roche Inc. hasgranted Cambio a PCR based DNA manufacturing license to make theSTARHFISH range of Chromosome Paints.

The STARHFISH range includes: Mouse Whole Chromosome-specific Probes,Human and Mouse Centromeric Probes, Detection Kits, Labeled and Sec-ondary Antibodies and ancillary products. We are also pleased to an-nounce that this range is now complemented by AL Technologies’ prod-ucts, which include Human Chromosome-specific ‘p’ and ‘q’ arm probes.

� STAR*FISH Human Whole Chromosome-Specific Probes

Cambio’s STAR*FISH system provides an excellent method for the identi-fication of single human chromosomes, and allows the detection of trans-locations and insertions on metaphase chromosomes. Marker chromo-somes can also be identified and complex karyotypes analysed. Probesare available for all chromosomes. To allow users to undertake moreambitious protocols, paints are now supplied in concentrated form withseparate hybridisation buffer. The individual paints come in packs of 10and 20 tests. All Cambio paint systems for whole human chromosomes arenow Cot-1 free. Ground breaking work by our collaborators in the Uni-versity of Cambridge has resulted in competitor DNA-free painting. ALTechnologies’ whole chromosome probes labelled with digoxigenin (DIG)are now available from Cambio.

� STAR*FISH Human Paint Boxes

The Cambio ‘Paint Box’ contains 3 tests for each chromosome presentedin one convenient palette, which is particularly useful for researchersneeding to test for each chromosome. STAR*FISH Paint Boxes are sup-plied in a ready-to-use format (45µl per vial).

� Multicolour FISH (M-FISH)

Multicolour FISH (M-FISH) allows the simultaneous visualisation of all hu-man chromosomes in a different colour and consequently allows the ex-amination of the human genome in a single hybridisation. The technologyis especially useful when specimen material is limited and numerous com-plex chromosomal rearrangements are involved. SKY and M-FISH are thetwo systems used to analyse multicoloured chromosomes and the systemsdiffer only in the way they capture and analyse the images. The multicolourprobe set consists of a mixture of combinatorially labelled chromosome-specific paints of all human chromosomes. These paints are derivedfrom DOP-PCR-amplified DNA from FACS-sorted chromosomes.

� Human Rainbow*FISH Probes

The human Rainbow*FISH probes visualise 6 chromosomes per set (1-6, 7-12, 13-18 and 16-Y). Covering the entire genome in just four experi-ments, the Rainbow*FISH probes are quick and easy to use. The probesutilise biotin, FITC and Cy3, so there is no need to buy new filters orsoftware. The probes come ready to use with hybridisation buffer.

� STAR*FISH Human Chromosome-SpecificArm Probes

AL Technologies’ probes for the ‘p’ or ‘q’ arm of each chromosome arelabelled with either biotin or digoxigenin (DIG). Please note that due tocross-hybridisation only the ‘q’ arm is available for chromosomes 13,14, 15, 21 and 22.

� STAR*FISH Human Chromosome-SpecificBand Probes

These specific band and oncogene probes are constructed frommicrodissected normal human metaphase chromosome DNA that has beendirectly amplified by PCR using a degenerate primer. The probes areavailable labelled with either biotin or digoxigenin (DIG).

� STAR*FISH Human Chromosome-Y-Specific Probes

Chromosome Y Euchromatic Probe – Biotin Labelled

This probe is an unpublished cosmid clone (LOR2.6, 9, Taylor & Wolfe),which can be used to detect the euchromatic portion of the Y chromo-some but not the pseudoautosomal region at the distal tip of Yp (and Xp)and not the distal Yq heterochromatin. It gives no signal elsewhere on thegenome. Use detection kit numbers 1066-K, 1082-KT, 1089-KB, 1090-KD,1043-KB or 1596-KC with this product.

Chromosome Y Heterochromatic Region Probe

To supplement our Chromosome Y Euchromatic probe and the whole chro-mosome Y paints, which do not paint heterochromatin, we also providesa Yqh-specific probe. Available with biotin, FITC or Cy3 labelling, it canbe used in conjunction with other STARHFISH paints and probes, andother STAR*FISH detection kits and reagents.

� STAR*FISH Human Chromosome-SpecificCentromeric Probes

A comprehensive range of biotin, Cy3 & FITC labelled human chromo-some-specific centromeric probes is now available from Cambio.

� STAR*FISH Human Chromosome Pan CentromericProbes

These DNA probes identify the centromeric region of each chromosome.They are ideal for the detection of aneuploidy and polyploidy, dicen-trics, tricentrics and other complex aberrations, as well as for generalnumerical chromosome analysis. All STAR*FISH Chromosome Paint sys-tems are carefully tested for their cytogenetic performance as in situhybridisation probes The paints are available in both concentrated and‘ready-to-use’ format. Storage at -20°C is recommended.

� STAR*FISH Human ChromosomePan-Telomeric Probes

We offer a probe specific to the telomeric region of all human chromo-somes. Implicated in the processes of cell division, telomeres are be-lieved to play a role in senescence and cancer. STAR*FISH telomericprobes are an invaluable tool to any researcher studying these areas.Available labelled with biotin, FITC or Cy3 the probes are tested forspecificity and signal intensity, and are provided with full experimentalprotocols.


� Harlequin*FISH Paints

Harlequin*FISH paints are derived from the application of chromosomepainting to comparisons of the genetics of a wide variety of species. This‘cross-species colour banding’ was developed to be applied to the analy-sis of the human genome. Created from two closely related gibbon spe-cies, the probes hybridise to different loci in the human genome.

Benefits:

• Whole genome analysis in a single multicolour FISH assay

• Combinatorial labelling generates 7 colours

• Unique ‘bar code’ for each chromosome

• Characterise gross and cryptic genomic rearrangements

• Detect inter-chromosomal translocations

• Interpret marker chromosomes and samples with poor

morphology

• Provide rapid and precise characterisations of leukaemias

• Detect and classify cancers

• Monitor therapeutic response

• Accessible, robust FISH tool

� STAR*FISH Primed In Situ Synthesis

Telomeric PRINS Kit

STAR*FISH PRINS (PRimed IN situ Synthesis) is a rapid and sensitivemethod of detecting sequences of DNA without requiring lengthy in situhybridisation protocols. The technique is basically a single-cycle PCRreaction, requiring a single specific primer and labelled dUTP/dNTPsolution. The key advantages of this method of in situ detection are theintensity and clarity of the signal and the speed of the protocol. Thesignal produced is intense and localised due to the fact that individualunincorporated nucleotides do not contribute significantly to background,and carefully chosen primers eliminate false signal emerging from otherloci. With its speedy protocol, results can be obtained in under 1 hour (alot faster than conventional FISH). Also, PCR requires few, if any, toxicreagents, unlike the need for formamide in FISH.

� STAR*FISH Mouse Whole Chromosome-SpecificProbes

STAR*FISH Mouse Whole Chromosome-Specific paint systems are pre-pared from PCR-amplified DNA obtained from FACS-sorted chromosomes.Mouse paints are now being used to develop model systems for studies ingenetics, mutagenesis, developmental cancer biology, and as human sur-rogates in studying the effects of genotoxic agents. Available in thisrange are whole chromosome paints for use in the detection of transloca-tion and trisomy in mice. Paints are available for chromosomes 1–19, Xand Y.

Mouse probes are available labelled with biotin, FITC or Cyanine 3, andare supplied with hybridisation buffer in packs of 10 and 20 tests. Unlessotherwise requested, mouse probes are supplied in the ‘ready-to-use’format.

� STAR*FISH Mouse Paint Boxes

In addition to our popular human paint boxes, we also offers 3 tests ofeach mouse whole chromosome paint in one convenient palette. Thisproduct was specifically designed for researchers needing to test foreach chromosome, and is also a useful resource when initialising a newpainting assay or service

� STAR*FISH Mouse Pan-Centromeric Probes

To complement our human pan-centromerics, ourrange includes mousepan centromeric probes. Manufactured and quality controlled to thesame high standards as our human probes, the mouse pan-centromericsare ideal for the detection of chromosome number aberrations in thisexperimental species.

FISH PaintsCYTOGENETICS

StarFish chromosomal Paints

� Mouse Rainbow*FISH Probes

The mouse Rainbow*FISH probes visualise 7 chromosomes per set (1-7, 8-14 and 15-Y). Covering the entire genome in just three experiments, theRainbowHFISH probes are quick and easy to use. The probes utilisebiotin, FITC and Cy3, so there is no need to buy new filters or software.

� X-Y FISH Bovine Sex Test Kit

X and Y chromosome paints were developed from sorted yak chromo-somes for sexing cattle spermatozoa. The procedure was evaluated usingthe Beltsville sperm sexing technology, which separates spermatozoa byflow-cytometry into X- and Y-bearing fractions. Using FISH, the methodis a simple, reliable and robust procedure for assessing the effective-ness of any separation technique of bovine X and Y spermatozoa.

Paints can also be used for interphase-FISH.

� Bovine Translocation (1;29) FISH Kit

The 1;29 balanced chromosome translocation is recognised to be themost common chromosome aberration in cattle causing reduced fertil-ity. The t(1;29) abnormality can be identified or excluded by conven-tional chromosome analysis, which is used widely as a screening test.

FISH analysis offers the advantage of identifying the translocation ininterphase nuclei and suboptimal chromosome preparations. It providesa rapid and unequivocal diagnosis.

� STAR*FISH Rat 12/y PaintsSTAR*FISH Pig x/y Paints

These highly-specific paints are suitable for a wide range of applica-tions. As with all our paints, they come with the Cambio guarantee ofquality, and the full technical support of the University of CambridgeMolecular Cytogenetics Laboratory.

� STAR*FISH Detection Kits

These Detection Kits have been specially designed to optimise thevisualisation of the Chromosome Paints. Due to popular demand, theyare provided separately from the Paints.

� Sonicated Human Genomic DNA

This product has been purified from human placental by phenol extrac-tion. Quality control (by electrophoresis) indicated the absence of pro-tein and RNA. It is suitable for eliminating background when performingSouthern and other types of hybridisation, genomic analysis, and libraryconstruction. Size range shows a medium 300–500bps. Supplied at10mg/ml.

� Proteinase K & Ribonuclease A (RNase A)

Please see page # 3 and page # 39


Genome Walking- APA™TechnologyNEW TECHNOLOGIES

APA Gene Technology

� Asymmetrical PCR Amplification (APA™) technology

Asymmetrical PCR amplification (APA™) represents a new generation of PCR technology developed by Bio S&T (U.S. patent pending).

PCR technology is widely used in molecular biology for DNA cloning, genomic typing and sequencing. It has also been applied in medical and pharmaceu-tical research, clinical diagnosis and human DNA fingerprinting. A variety of PCR permutations have been developed since its conception. However, acommon limitation among these variations is the need to know the DNA sequences flanking the region of interest. Thus, DNA amplification has been limited totemplates with known sequences. APA™ is designed specifically to overcome this limitation.APA™ technology is capable of selectively or randomly amplifying any unknown DNA sequences. The APA™ procedure includes two steps (see figure 1):

In step 1, particular primer sets named degenerated random tagging (DRT) primers

are involved in the reaction. Each DRT primer consists of three components respec-

tively, each containing a 4-6 nucleotide arbitrary sequence at the 3’ end, a bi-nucle-

otide degenerated sequence and a tagging primer binding sequence. The functions of

the DRT primers are to randomly bind denatured DNA templates, lead single strand

DNA amplification, and provide a tagging primer binding position in the 5’ region. The

annealing temperature during this step ranges from 35-42oC with an optimum of 37oC

for many species. In step 2 of the APA™ procedure, a specific primer and tagging

primer are used in a reaction performed at a higher annealing temperature (higher

than 57oC).

One of the most effective uses of APA™ Technology is gap filling in genome integration.

Conventional gap filling is often laborious, time consuming and ineffective given that

too much DNA template is utilized in each reaction. Through APA™ Technology the

sequencing process to walk the BAC clones is extremely simple and reliable with the

additional advantage that only a minute amount of DNA is needed for the reaction.

Usually, BAC End sequencing is usually limited by two factors: 1) a large amount of DNA,

usually more than 5 ug is needed for each reaction 2) the BAC vector is typically a single

copy vector, making it hard to extract large amounts of DNA from a BAC clone. Our

revolutionary APA™ Technology has overcome these limitations. With this absolutely

fresh approach, only 2-5 ng of template DNA are needed for each reaction, almost 1000

times less than required by the conventional procedure.

� APAgene™ Genome Walking Kit

Can be used for: gap filling, localized cloning of genomic DNA, isolating promoter and regulatory sequences corresponding to cloned cDNAs, 5’ and 3’RACE of first-strand cDNAs, and identifying intron/exon junctions.

� APAgene™ Locator Kit

Can be used for: identifying transgene/genomic DNA junctions, bi-directional sequence extension of sequence-tagged sites (STSs) and expressedsequence tags (ESTs), identifying gene traps and transposon-insertion locations.

� APAgene™ BAC End Amplification Kit

Can be used for: insert-end amplification of large clones such as P1, YAC and BAC as well as localized sub-cloning of large clones such as P1, YAC andBAC.


BT600 APAgene™ 6 walksGenome Walking Kit




BT700 APAgene™ 6 walksGenome Locator Kit




BT500 APAgene™ BAC 6 walksEnd Amplification Kit



Figure 1: Schematic Representation of the APA Technology


A Novel Cloning Vector - pLivSelect®Cloning TechnologyNEW TECHNOLOGIES

Cloning Vector

� pLivSelect®Cloning Technology

pLivSelect® technology (patent pending No. 2,335,412) is the latest tech-nology in DNA cloning. Compared with conventional DNA cloning technolo-gies, which depend on blue/white selection for recombinants, pLivSelect®

is a revolution in DNA cloning.

In conventional cloning technologies, the lac operon contains a partiallacZ sequence coding for the N-terminal portion of the b-galactosidasegene. The C-terminal portion is contributed by the host via a-comple-mentation. If a DNA insert is not introduced into the lacZ gene, anenzymatically active b-galactosidase will be generated via the a-comple-mentation of the N-terminal portion with the C-terminal portion con-tributed by the host strain. The functional b-galactosidase will thencleave X-gal resulting in an insoluble blue substance forming blue colo-nies. If a DNA insert is introduced into the lacZ gene, transcription ofthe N-terminal portion of the b-galactosidase will be interrupted, pre-venting a-complementation and consequently cleavage of X-gal. The re-sulting colonies are colorless.

Our revolutionary pLivSelect® cloning vector provides an innovativedirect selection system for recombinant identification. The key partof this innovative cloning system comprises:

• A lacI repressor sequence that has been engineered to

contain multiple cloning sites (MCS);

• A lac-ABR operon where the antibiotic resistant gene is

placed under the regulation of the lac operon.

If there is no DNA insertion in the MCS area of the lacI sequence, lacI hasnormal repressor function that will inhibit lac promoter activity for b-lactamase synthesis. Therefore, cells that harbor the non-recombinantvector cannot survive on agar plates containing chloramphenicol. In con-trast, if a DNA insert has been introduced into the MCS region of the lacI

sequence, the chlorampehnicol resistance gene will be normally expressedowing to the absence of lacI repressor activity. Cells that contain a vec-tor carrying an exogenous DNA fragment will survive on agar plates con-taining the antibiotic. pLivSelect® is thus a direct antibiotic-based se-lection system for recombinant identification. pLivSelect®-PCR CloningKits can be applied for cloning PCR products amplified using either Taqor Pfu DNA polymerases (those which generate either sticky or bluntends).

Figure 1: PLivSelect Vector Map

� pLivSelect® cloning vector

The pLivSelect®-PCR vector is derived from the pLivSelect®-I vector(Cat#BC101). The XbaI site in the MCS region of the original vector hasbeen replaced by a PmlI site. Therefore, the pLivSelect®-PCR vectorcontains two PmlI sites flanking the PCR insertion site (HpaI) so that thePCR insert can easily be excised from the vector through a single PmlIdigestion. The pLivSelect®-PCR vector provided in the pLivSelect®-PCRCloning Kit has been linearized using Hpa and processed through a uniqueprocedure so that the DNA vector can be used to clone all types of PCRproducts. Therefore, pLivSelect®-PCR Cloning Kits can be applied forcloning PCR products amplified using either Taq or Pfu DNA polymerases(those which generate either sticky or blunt ends).

Choose between pLivSelect®-II Cloning Kit A, B or C depending uponthe restriction sites into which you would like to clone your gene.

The following are the contents of pLivSelect®-II Cloning Kit A, Band C:

• Linearized and dephosphorylated pLivSelect-II vector DNA,100 ng/µl

• T4 DNA ligase, 5 U/µl

• 10X T4 ligase buffer

• E. coli DH10B glycerol stock

pLivSelect®-II Cloning Kit R-A, R-B and R-C

Contains 40 Ready-to-go cloning reaction mixtures (for a 20 µl ligationreaction).Each lyophilized reaction mix contains

• 100 ng of pLivSelect.-II vector DNA

• T4 ligase buffer components

• T4 DNA ligase

• E. coli DH10B agar stock

� pLivSelect®-II-PCR Cloning Kit L

(With and Without competent cells) Optimized to enhance blunt-endligation (all types of PCR products with blunt ends or 3‘-A overhangsticky ends can be cloned with high efficiency).

� pLivSelect®-II-PCR Cloning Kit

Optimized to enhance blunt-end ligation (all types of PCR products withblunt ends or 3‘-A overhang sticky ends can be cloned with high effi-ciency).

Transformation

All E. coli strains that do not express the lac repressor (lacI or lacI q)

may be used for transformation. If you use a strain that expresses thelac repressor, your transformants WILL NOT grow on agar plates con-taining chloramphenicol.


A Novel Eukaryotic Expression System-LEXSYNEW TECHNOLOGIES


� Leishmania Expression System

The Leishmania Expression System represents the combination of easy handling known from bacterial expression systems with the potential of aneukaryotic protein expression/folding/ modification system. The protozoan host Leishmania tarentolae was isolated from lizard and is non-humanpathogenic (Biosafety level 1).

For high level expression target genes are supplied with specific splicing signals and integrated into a strongly transcribed chromosomal site ormaintained episomally. The expression constructs are made in E. coli and introduced into L. tarentolae by electroporation.

Up to four selection markers can be used simultaneously for coexpression of multisubunit proteins in one strain or expression enhancement.General and secretory expression vectors are available.

The selected recombinant strains can be cultivated as static or agitated cultures and grow to high cell densities in standard bacteriological mediawithout sera. The cells can be easily lysed by sonication or detergents.

One main advantage of the Leishmania Expression System is the potential for mammalian-type posttranslational modification of heterologoustarget proteins, such as glycosylation, phosphorylation, prenylation etc.

• Amplification of target gene from cDNA as Bgl II / Nco I / Xho I x Xho I / Pac I / Not I compatible cassette

• Insertion of target gene into Leishmania expression vector

• Transfection of Leishmania tarentolae host strain by electroporation

• Selection of recombinant strains with Nourseothricin/Hygromycin/Bleomycin/Neomycin

• Confirmation of genetic structure of recombinant strain by diagnostic PCR

• Cultivation of expression strain and evaluation of expression level of target protein

• Purification and characterization of target protein

Reference:Breitling et al. (2002) Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Prot. Expr. Purific. 25:209.

Fig. 1 : The pF4X1.4 general expression plasmid family is availablewith sat, hyg, ble or neo marker genes for selection with Nourseothricin,Hygromycin B, Bleomycin or Neomycin. Instead of a poly-linker, thetarget gene replaces a stuffer fragment, allowing control for properrestriction. The Nco I site overlaps the ATG of the target gene. 5’ int and3’ int are regions for homologous recombination into the host chromo-some following linearization of the expression plasmid with Swa I; utr1,utr2 and utr3 are optimized gene flanking non-translated regions pro-viding the splicing signals for posttranscriptional mRNA processing forexpression of target and marker genes in L. tarentolae.

Figure 2: The pSP1.4sat secretory expression plasmid family allowsthe use of a signal peptide which has proven to function for extracellularsecretion and correct processing in L. tarentolae. Signal peptide codingregions from human epo gene or from L. tarentolae gp63 gene are avail-able. Instead of a polylinker, the mature part of the target gene replacesa stuffer fragment, allowing control for proper restriction. The Kas Isite overlaps the signal peptide cleavage site. 5' int and 3' int are regionsfor homologous recombination into the host chromosome following linear-ization of the expression plasmid with Swa I. For secretory expressionwith the homologous signal peptide of the target protein the pF4X1.4general expression plasmid family (Fig. 1) can be used.


Protein Expression in a new system-LEXSYNEW TECHNOLOGIES


� Proteins expressed in L. tarentolae at JenaBioscience:

• Cytosolic proteins:

human transcription factors of the proto-oncogene family:Miz-1, c-Myc, Max, Mad1,

human Cu/Zn superoxide dismutase, ß-galactosidase, T7 RNApolymerase, green fl uorescent protein, glutathione S-trans-ferase.

• Membrane proteins:

human bradykinin receptor, Rab7-GTPase, Type I MBP.

• Secreted proteins:

human erythropoietin, single chain antibodies, humaninterferon •

� Gene Expression Starter Kit

If you prefer to perform the complete transfection and expression pro-cedure yourself, Jena Bioscience offers a Starter Kit, containing allessential components to engineer a Leishmania expression strain. Thefollowing steps are described in an easy to follow manual:

1. Amplifi cation of target gene as Bgl II / Nco I / Xho I x Xho I /Pac I / NotI compatible cassette

2. Insertion of target gene into Leishmania expression vector by stan-dard methods in E. coli

3. Transfection of Leishmania tarentolae host strain by electroporation

4. Selection of recombinant strains with Nourseothricin / Hygromycin /Bleomycin / Neomycin

5. Confirmation of genetic structure of recombinant strain by diagnos-tic PCR

6. Cultivation of expression strain and evaluation of expression level oftarget protein


EGE-101 Constitutive LEXSY Starter Kitbleomycin selection(contains vector pF4X1.4ble) 1 Kit

EGE-102 Constitutive LEXSY Starter Kithygromycin selection(contains vector pF4X1.4hyg) 1 Kit

EGE-103 Constitutive LEXSY Starter Kitneomycin selection(contains vector pF4X1.4neo) 1 Kit

EGE-104 Constitutive LEXSY Starter Kitnourseothricin selection(contains vector pF4X1.4sat) 1 Kit

Kit Contents• Leishmania tarentolae recipient strain as live submerse culture• 50 ml completed medium for initial inoculations• Components for preparation of 1-liter growth medium• General Expression vector with sat marker (Nourseothricin

selection)• 50 ml electroporation buffer• Primer sets for insert sequencing and diagnostic PCR• Manual (the manual is also available for download in PDF format

from our web site at www.jenabioscience.com)


EGE-201 pF4X1.4ble Constitutive expression vector 5 µg (50 µl)

EGE-202 pF4X1.4hyg Constitutive expression vector 5 µg (50 µl)

EGE-203 pF4X1.4neo Constitutive expression vector 5 µg (50 µl)

EGE-204 pF4X1.4sat Constitutive expression vector 5 µg (50 µl)

Host strains, Growth media, antibiotics, additives and primers available.

Please inquire for pricing of custom transfection services

Important Licensing Information:

This service is licensed for non-commercial research only.

Commercial use of this expression system requires separate licensing.

Cloning of customer genes in Leishmania expression vectors, developmentof protein purification protocols, pilot expressions and proteinpurification can be performed with a separate contract.

� High level Inducible expression of targetproteins in LEXSY

Use of heterologous T7 polymerase-TET repressor system for high levelprotein synthesis:


EGE-120 Inducible LEXSY Starter Kitbleomycin selection(contains vector pTUBAPX1.4ble) 1 Kit

EGE-121 Inducible LEXSY Starter Kitneomycin selection(contains vector pTUBAPX1.4neo) 1 Kit

Expression vectors also available separately

� Secretory expression of target proteins in LEXSY

Efficient protein secretion with function proved signal peptides forprotein targeting:


EGE-110 Secretory LEXSY Starter Kit EPO 1 Kitsignal peptidenourseothricin selection(contains vector pF4SPepoX1.4sat)

EGE-111 Secretory LEXSY Starter Kit LMSAP 1 Kitsignal peptidenourseothricin selection(contains vector pF4SPlmsapX1.4sat)

EGE-113 Secretory LEXSY Starter Kit LMSAP 1 Kitsignal peptidehygromycin selection(contains vector pF4SPlmsapX1.4hyg)

EGE-114 Secretory LEXSY Starter Kit LMSAP 1 Kitsignal peptideneomycin selection(contains vector pF4SPlmsapX1.4neo)

Expression vectors also available separately

� Leishmania tarentolae Cultivation Starter Kits

The Leishmania tarentolae Cultivation Starter Kit is the easiest way toestablish a Leishmania tarentolae cell culture in your own laboratory.The kit contains everything to start your work with Leishmania tarentolae

(strain as live suspension culture, media, cell culture flasks and cryo vialswith glycerol).


LT-102 LEXSY Cultivation Starter Kit P10 1 Kit(contains laboratory strain P10;use for constitutiveand secretory expression vectors)

LT-111 LEXSY Cultivation Starter Kit T7-TR 1 Kit(contains T7-TR strain expressingbacteriophage T7 RNA polymerase andTET repressor; use for inducibleexpression vectors)

www.biotechdesk.com T : 040-27017477 / 65916167 F : 040-27016168 [email protected]

1 kb Ladder ................................................................ 11100 bp + 1.5 kb DNA Ladder ............................................ 11100 bp Ladder ............................................................. 112’-Deoxyribonucleoside-5’-Triphosphate Solutions ................ 11200 bp Ladder ............................................................. 12293 cell nuclear extract ................................................ 1232'-dATP ...................................................................... 112'-dCTP ...................................................................... 112'-dGTP ...................................................................... 112'-dNTP (mix of all 4) .................................................... 112'-dTTP ...................................................................... 112'-dUTP ...................................................................... 112'-Fluoro-dCTP Solution .................................................. 322'-Fluoro-dUTP Solution .................................................. 322-Way™ Cap, m7G[5’]pppp[5’]m7G .................................. 312X Tissue & Cell Lysis Buffer ............................................. 33'-RACE ............................................................... 132,15150 bp Ladder ............................................................... 12500 bp Ladder ............................................................. 125'-RACE ............................................................... 132,1518-Oxoguanine-DNA Excision Mix ........................................ 41

AAbl SH3 domain .......................................................... 106ABTS ......................................................................... 91acridine orange .......................................................... 137actinomycin D ............................................................ 137Additive screen ............................................................ 79Additives .................................................................... 79ADHP ......................................................................... 91adrenergic receptors .................................................. 101Adrenocorticotrophin peptides ...................................... 130AEC fizzing tablets ...................................................... 113AF6-RBD ................................................................... 100Affinity chromatography resins, columns ............................ 58Agarase ...................................................................... 17Agarose Gel-Digesting Preparation, GELase™ ....................... 17Agouti related peptides ............................................... 130Akt1/PKBa ........................................................... 106-110Akt1/PKBb ........................................................... 106-110Alcian Blue ................................................................ 141Alkaline Phosphatase (CIP) .............................................. 42Alkaline Phosphatase assay kits ......................................... 89Alkaline Phosphatase, APex™ Heat-Labile ............................ 42Alkaline Phosphatase ...................................................... 42Amino acids ............................................................... 127Amino acids-Boc labelled .............................................. 127Amino acids-Fmoc labelled ............................................ 127Amino hexyl CTP Sepharose ............................................ 58Amino hexyl dCTP Sepharose .......................................... 58Amino hexyl dGTP Sepharose .......................................... 58Amino hexyl dITP Sepharose ........................................... 58Amino hexyl dUTP Sepharose .......................................... 58Amino hexyl dXTP Sepharose .......................................... 58Amino hexyl GTP Sepharose ............................................ 58Amino hexyl ITP Sepharose ............................................. 58Amino hexyl UTP Sepharose ............................................ 58Amino hexyl XTP Sepharose ............................................ 58Amino hexyl ATP Sepharose .............................................. 58Amino hexyl dATP Sepharose ........................................... 58Amino octyl ATP Sepharose .............................................. 58Amino octyl CTP Sepharose ............................................. 58Amino octyl dATP Sepharose ............................................ 58Amino octyl dCTP Sepharose ........................................... 58Amino octyl dGTP Sepharose ........................................... 58Amino octyl dITP Sepharose ............................................ 58Amino octyl dUTP Sepharose ........................................... 58Amino octyl dXTP Sepharose ........................................... 58Amino octyl GTP Sepharose ............................................. 58Amino octyl ITP Sepharose .............................................. 58Amino octyl UTP Sepharose ............................................. 58Amino octyl XTP Sepharose ............................................. 58Amino phenyl ATP Sepharose ............................................ 58Aminoallyl- UTP ............................................................ 34Ammonium Acetate 5M Solution ........................................ 17AmpliCap-MAX™ SP6 High YieldMessage Maker RNA Kit .................................................. 31AmpliCap-MAX™ T3 High Yield Message Maker RNA Kit ........... 31AmpliCap-MAX™ T7 High Yield MessageMaker RNA Kit ............................................................. 31AmpliCap™ SP6 High Yield Message Maker RNA Kit ................. 31AmpliCap™ T3 High Yield Message Maker RNA Kit .................. 31AmpliCap™ T7 High Yield Message Maker RNA Kit .................. 31Amplification, DNA see PCRAmpligase® DNA ligase Kit ............................................... 43

Ampligase® Thermostable DNA Ligase ................................. 43AmpliScribe™ SP6 High Yield Transcription Kit ..................... 30AmpliScribe™ T3 High Yield Transcription Kit ...................... 30AmpliScribe™ T3-Flash™ Transcription Kit ........................... 30AmpliScribe™ T7 High Yield Transcription Kit ...................... 30AmpliScribe™ T7-Flash™ Transcription Kit ........................... 30AmpliTherm™ DNA Polymerase, MasterAmp™ ........................ 8Amyloid - vital stains ..................................................... 93Amyloid peptides ........................................................ 130Analytical services ...................................................... 133AnaPrep AEC substrate system ....................................... 115AnaPrep IHC Histain kit ................................................ 115ANS ......................................................................... 141Antibiotic ................................................................. 130Antibodies, custom synthesis ......................................... 133antibodies, primary .................................................... 113antibodies, secondary .................................................. 113Antibody diluent ......................................................... 115antioxidants .............................................................. 126APA technology ........................................................... 151APex™ Heat-Labile Alkaline Phosphatase .............................. 42A-plus Poly (A) Polymerase Tailing kit ................................ 31Apoptosis Blocking peptides .......................................... 130Apoptosis ................................................................ 94-95ARCA Cap Analog, 3’-O-Methyl-m7G[5’]ppp[5’]G .................. 31ARCA, 3’-O-Methyl-m7G[5’]ppp[5’]G ................................ 19aRNA Amplification .................................................... 33-35ArrayPure Nano-scale RNA Purification kits ......................... 4Arrest-in Transfection reagent ........................................ 36artemin .................................................................... 125ATP (Adenosine-5'-Triphosphate) ...................................... 32ATP Solution ................................................................ 32Autocamtide-2, substrate for CaMK ................................ 109Autocamtide-3, substrate for CaMK ................................ 109Automated Cycle Sequencing ........................................... 56

BB stearothermophilus (rBst) DNA Polymerase ........................ 7β1-adrenergic receptor-G protein fusion ......................... 101β1-adrenergic receptors .............................................. 101β2-adrenergic receptors .............................................. 101β2-adrenergic receptors-G protein fusion ........................ 101BAC Clones .................................................................. 28BAC Cloning ............................................................. 19-21BAC Genomic library ................................................... 131BAC library construction .............................................. 131BAC VectorsBACMAX DNA Purification kit ........................................ 4,20Bacterial Protein Extraction Solution ................................. 57Bacteriophage Lambda ............................................... 12,21BAC-Tracker™ Supercoiled DNA Ladder .............................. 20BAPTA ...................................................................... 144BCIP/INT .................................................................. 113Bcl-10 ...................................................................... 121BDNF, brain derived neurotrophic factor ......................... 125beadbeater ................................................................. 67Benchtop freezer ......................................................... 66Beta amyloid antibodies ................................................. 93Beta amyloid fluorescence sampler kits .............................. 93Beta amyloid peptides ................................................... 93Beta amyloid tracers ..................................................... 93Beta-secretase assay kit ................................................. 90Beta-secretase assay-substrates ....................................... 90bio pulverizer .............................................................. 68Bioactive peptides ...................................................... 130biocytin C2 maleimide .................................................. 114Biocytin .................................................................... 114Bioprocessing, Protein & Nucleic Acid ............................... 57Biotin Quantitation kit, HABA ........................................ 115Biotin, thiol reactive ................................................... 114Biotin-fluorophore tagged ............................................. 115Biotins, amino containing .............................................. 114Biotins, amino reactive ................................................ 114Biotin-X .................................................................... 114Biotin-X-X-NHS ............................................................. 35Biotinylation kit for proteins ......................................... 114BioTrack Human Gene subsets .......................................... 25BLA RP-1 Reverse Primer ............................................... 50bLyS/BAFF, B-lymphocyte stimulator/B-cell activating factor 125BMP-2, bone morphogenetic protein-2 ............................ 124BMP-4, Bone Morphogenetic protein 4 ............................. 123Bombesins ................................................................. 130BOP ......................................................................... 128BPDEtide, substrate for PKG ......................................... 109Bradykinins ............................................................... 130

INDEX

i


Brahma-related Gene 1 protein ...................................... 122BRCA1 ...................................................................... 121BrdU ....................................................................... 138BrdUTP .................................................................... 138Brucella ORF Collection ................................................... 26BSA, maleimidyl .......................................................... 115BuccalAmp™ DNA Extraction Kit ......................................... 1Building Blocks ........................................................... 129

CC jejuni ORF Collection ................................................... 26C.elegans ORF Collection ................................................. 27C.elegans Promoter Collection .......................................... 27C.elegans RNAi library ................................................... 36Calcein ..................................................................... 143Calcitonin related peptides ........................................... 130Calcium binding proteins .............................................. 126Calcium indicators, non luminiscent ................................ 144Calcium indicators, UV excitable .................................... 143Calcium indicators, Visible light excitable ................... 143,144Calcium/calmodulin-dependent kinases ............................. 107Calmodulin ................................................................ 126Calpain-substrate ......................................................... 87Cancer research Blocking peptides ................................. 130Candida albicans Genomic Library .................................... 27Cap analogs ................................................................. 31Capping Kits, RNA ......................................................... 31Caspase 3 assay kit ....................................................... 94Caspase 7 assay kit ....................................................... 94Caspase antibodies ........................................................ 95Caspase assay kits ......................................................... 94Caspase assay substrates ................................................ 95Caspase Profiling kit ...................................................... 94Catch-All™ Sample Collection Swabs .................................... 1Cathepsin- substrate ..................................................... 87CCAAT-box-binding Transcription factor1 ......................... 121CD40 ligand ............................................................... 125cdc25A ..................................................................... 111Cdc4 ........................................................................ 298cDNA Clones ......................................................... 16,25-27cDNA Cloning ............................................................ 16-17cDNA Libraries ......................................................... 25-27cDNA library construction ............................................. 131cDNA Synthesis ......................................................... 14,15Cell adhesion assays ..................................................... 133Cell counting kit .......................................................... 148Cell Cytotoxicity assay kit ............................................. 148Cell permeable peptides ............................................... 130Cell proliferation assays ............................................... 133Cell viability and Proliferation assay kit ........................... 148Cell viability assays, fluorogenic substrates .................. 145-148Cell viability, monitoring cellular functionsby fluorescent stains ............................................... 145-148c-fos ........................................................................ 121Chiller ........................................................................ 66CHK2 (Checkpoint kinase 2) ........................................... 107Chromosome paints ................................................ 149-150CircLigase™ ssDNA Ligase .............................................. 122c-jun ....................................................................... 121CK 2 (Casein kinase 2) .................................................. 107CK2a ................................................................... 107,108CK2b .................................................................. 107,108Cloning kit, PCR, pLivselect vector ................................. 152Cloning vector, pLivselect ............................................. 152c-myc ...................................................................... 121c-myc-anti, antibody ................................................... 113CNTF, Ciliary Nerotrophic factor .................................... 125Coelentrazine ............................................................. 144Colony Fast-Screen™ Kit (PCR Screen) ............................... 22Colony Fast-Screen™ Kit (Restriction Screen) ..................... 22Colony Fast-Screen™ Kit (Size Screen) ............................... 22Competent Cells ........................................................ 23-24Complement Component 5a receptors .............................. 103Congo Red1 ................................................................. 41CopyControl™ BAC Cloning Kit (BamH I) .............................. 19CopyControl™ BAC Cloning Kit (EcoR I) ............................... 19CopyControl™ BAC Cloning Kit (Hind III) ............................. 19CopyControl™ cDNA, Gene & PCR Cloning Kit ....................... 16CopyControl™ Fosmid Library Production Kit ...................... 18CopyControl™ HTP Fosmid Library Production Kit ................ 18CopyControl™ Induction Solution ............................... 16,18,19CopyControl™ pCC1BAC™ BamH I Cloning Ready Vector ........ 19CopyControl™ pCC1BAC™ EcoR I Cloning Ready Vector .......... 19CopyControl™ pCC1BAC™ Hind IIICloning Ready Vector ..................................................... 19

CopyCutter EPI400 Cells ............................................. 23,24Core Enzyme, E coli RNA Polymerase .................................. 29Corticotrophin releasing factor ...................................... 130Cosmid Cloning ............................................................. 21Coupling reagents ....................................................... 128c-Raf ................................................................... 99-100CREBtide, substrate for PKA ......................................... 108Crosstide, substrate for Akt/PKB ................................... 109Cryo crystallization ....................................................... 82Crystallization- Tape, Grease .......................................... 81Crystallization-covers slides ............................................ 81Crystallization-plates ..................................................... 81CTP (Cytidine-5'-Triphosphate) ........................................ 32CTP ........................................................................... 32Custom gene synthesis .................................................. 131Custom peptide synthesis ......................................... 130,132Custom synthesis of Fluorescent probes ............................ 132Cuvettes ................................................................. 73,75Cycle Sequencing ........................................................ KitsCyclin dependent kinases .............................................. 107Cytochrome P450 assay substrates .................................... 92Cytomegalovirus proteins .............................................. 117Cytotoxicity assays ..................................................... 133

DDAB ........................................................................... 91Dansyl chloride ........................................................... 134DAPI ........................................................................ 137D-Biotin .................................................................... 114DCC ......................................................................... 128Detergents .................................................................. 79DHFR-1 FP-1 Forward Sequencing Primer ..................... 52,55DHFR-1 RP-1 Reverse Sequencing Primer ...................... 52,55DIBOC ...................................................................... 128DIC ......................................................................... 128DIEA ........................................................................ 128DMAP ....................................................................... 128DNA & RNA Endonucleases ................................................ 40DNA & RNA Exonucleases ................................................. 40DNA Amplification, ................................................. see PCRDNA Binding Proteins ..................................................... 37DNA Endonucleases ......................................................... 38DNA Exonuclease I, E coli ................................................. 38DNA Exonuclease III , E coli .............................................. 38DNA Exonuclease VII, E coli .............................................. 38DNA Exonucleases ...................................................... 38,39DNA extraction ............................................................ 1-4DNA Fragment 2X Precipitation Solution ............................. 17DNA Glycosylases & Excision Mixes ..................................... 41DNA Markers ............................................................ 11,12DNA Polymerase I, E coli ................................................ 7-8DNA Purifcation from Plant .............................................. 2DNA Purification from Blood ............................................. 2DNA Purification from Faeces ........................................... 2DNA Purification from Leaf .............................................. 2DNA Purification from Soil ............................................... 2DNA Purification from Water ............................................ 2DNA Purification .......................................................... 1-4DNA Sequencing ........................................................ 55,56DNase Af ..................................................................... 41DNase, Plasmid-Safe™ ATP-Dependent .............................. 136DNP ......................................................................... 139dNTP ......................................................................... 11Dopamine receptors .................................................... 102Dr1, down regulator of transcription ............................. 1122Drosophila Gene Collection .............................................. 26Drosophila RNAi library .................................................. 36DuraScribe™ SP6 Transcription Kit ................................... 32DuraScribe™ T7 Transcription Kit ..................................... 32Dynorphins ................................................................ 130

EE coli RNA Polymerase Core Enzyme .................................. 29E coli RNA Polymerase Holo Enzyme (sigma saturated) ........... 29E coli DNA Endonuclease IV .............................................. 38E2F-1 ....................................................................... 121EasyLyseTM Bacterial Protein Extraction Solution ................. 57EDANS ...................................................................... 134EDC128EGF Receptor, substrate for MAPK ................................. 110EGF, Epidermal Growth Factor ....................................... 125EGTA ....................................................................... 144EG-VEGF, Endocrine Gland-derived vascular endothelial growth factor .......................................... 125Elastase-substrate ......................................................... 87

INDEX

ii


Electroblotter .......................................................... 62-63Electrophoresis - horizontal, submarine ......................... 59-60Electrophoresis- vertical ................................................ 61Electrophoresis-power supplies ........................................ 61Electrophoresis-Sequencing gels ....................................... 62Electroporation Cuvettes ............................................ 22,73ELISA substrates ......................................................... 113Endexins ................................................................... 130End-It™ DNA End-Repair Kit ............................................. 17Endorphins ................................................................ 130Endothelins ................................................................ 130Enkephalins ................................................................ 130Enzyme Storage Buffer ................................................... 12Eosin ........................................................................ 141EPAC-1 ....................................................................... 99EpiFOS™ Fosmid Library Production Kit ............................. 19Epi-Grids Colony Grid Templates ...................................... 22EPO, erythropoetin ..................................................... 123Epstein Barr Virus proteins .......................................... 117ERK1 (extra cellular related kinase) ................................ 108ERK2 (extracellular signal-regulated kinase) ...................... 108Estrogen receptor ....................................................... 123ESTs ........................................................................... 25Ethidium bromide ....................................................... 137Exo/S1 kit ................................................................... 41ExtractMaster Fecal DNA Extraction kit ............................... 2EZ::TN™ <blaM/R6Kγori> Transposon ................................. 50EZ::TN™ <DHFR-1> Insertion Kit ................................... 50,55EZ::TN™ <DHFR-1> Tnp Transposome™ .............................. 52EZ::TN™ <DHFR-1> Transposon ....................................... 52EZ::TN™ <KAN-2> Insertion Kit ..................................... 50,55EZ::TN™ <KAN-2> Tnp Transposome™ Kit ............................ 52EZ::TN™ <KAN-2> Transposon .......................................... 52EZ::TN™ <Not I/KAN-3> Transposon ................................... 54EZ::TN™ <oriV/KAN-2> Insertion Kit .................................. 49EZ::TN™ <oriV/KAN-2> Transposon .................................... 49EZ::TN™ <R6Kγori/KAN-2> Insertion Kit ............................. 50EZ::TN™ <R6Kγori/KAN-2> Transposon .............................. 50EZ::TN™ <T7/KAN-2> Promoter Insertion Kit ....................... 49EZ::TN™ <T7/KAN-2> Transposon ..................................... 49EZ::TN™ <TET-1> Insertion Kit ......................................... 55EZ::TN™ <TET-1> Transposon ........................................... 55EZ::TN™ Beta-Lactamase Fusion Kit ................................... 50EZ::TN™ In-Frame Linker Insertion Kit ............................... 54EZ::TN™ Plasmid-Based Deletion Machine ............................ 54EZ::TN™ pMOD™-2<MCS> Transposon

Construction Vector ................................................. 51EZ::TN™ pMOD™-3<R6Kγori/MCS>

Transposon Construction Vector .................................. 51EZ::TN™ Transposase ................................................ 49-55EZ::TN™<R6Kγori/KAN-2> Tnp Transposome™ ..................... 52EZ::TN™Protein Truncation Kit ........................................ 53EZ-Tn5™ <R6Kγori/KAN-2> Insertion Kit ............................. 50EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit ....................... 49EZ-Tn5™ γ -Lactamase Fusion Kit ....................................... 50

FFailSafe™ GREEN Real-Time PCR PreMix

Selection Kit ........................................................... 10FailSafe™ GREEN Real-Time PCR System .............................. 10FailSafe™ PCR 2X PreMix A to Premix L ............................... 6FailSafe™ PCR PreMix Selection Kit .................................... 6FailSafe™ PCR System ..................................................... 6FailSafe™ PROBES Real-TimePCR PreMix Selection Kit ................................................ 10FailSafe™ PROBES Real-Time PCR System ........................... 10FailSafe™ Real-Time GREEN Capillary PCR PreMix Selection Kit 10FailSafe™ Real-Time GREEN PCR Capillary System ................. 10FAM ......................................................................... 135Farnesoid X-activated receptor ..................................... 123Fast-Link™ DNA Ligation Kit ............................................. 43FDG ........................................................................... 89FDP, fluorogenic substrate for phosphatase ....................... 112FDP ........................................................................... 89FGF-11 ....................................................................... 21FGF-21 ....................................................................... 21FGF-91 ....................................................................... 25Fibronectin fragments ................................................. 130Finding Signal PeptidesFirst strand synthesis .................................................... 14FISH kit, bovine, (1,29) translocation ......................... 149-150FISH probes, Harlequin ........................................... 149-150FISH probes,Human chromosome Pan-telomeric ............................. 149-150FISH probes,

Human chromosome-arm specific .............................. 149-150FISH probes, Human chromosome-specific

band specific .................................................. 149-150FISH probes, Human chromosome-specific

centromeric .................................................... 149-150FISH probes, Human chromosomeY-specific .................. 149-150FISH probes, Human paint boxes ................................ 149-150FISH probes, Human Rainbow .................................... 149-150FISH probes, mouse paint boxes149-150FISH probes, mouse, pan-centromeric probes .............. 149-150FISH probes, Mouse, rainbow ................................... 149-150FISH probes, mouse, whole chromosome-specific probes . 149-150FISH probes, Multicolour FISH ................................... 149-150FISH probes, pig, X/Y ............................................. 149-150FISH probes, rat, 12/Y ............................................ 149-150FISH probes, whole chromosome specific ..................... 149-150FISH probes .......................................................... 149-150FISH, detection kits ................................................ 149-150FITC ........................................................................ 135FLT3 ligand, Fms-like Tyrosine kinase 3 ligand .................... 123Fluo-3 ...................................................................... 143Fluo-5F ..................................................................... 143Fluo-5N ..................................................................... 143Fluorescamine ............................................................ 141Fluorescent dyes .................................................... 134-141Fmoc Biocytin ............................................................ 114Fmoc-Osu .................................................................. 128Formyl Peptide receptors ............................................. 102Fosmid Cloning ......................................................... 18-19Fosmid Library Production ......................................... 18,19FosmidMAX DNA Purification kit ........................................ 4Freezer storage boxes ................................................... 66FRET probes, DABSYL based .......................................... 138FRET probes, EDANS based ........................................... 139Ftase ....................................................................... 100Fugu IMAGE cDNA .......................................................... 26Full Range DNA Ladder .................................................... 11Fura-2 ...................................................................... 143

GGα ........................................................................... 99GAL4-AH, GAL4 fused to alpha helix ................................ 121GAL4-E1A .................................................................. 121GAL4-Sp1Q ................................................................ 122GAL4-VP16 ................................................................ 121Galactosidase substrate ................................................. 89Galanins .................................................................... 130GAP (GTPase activation Protein) ...................................... 99Gastrins ................................................................... 130G-CSF ................................................................. 123,124Gel Blot papers ............................................................ 63Gel extraction .............................................................. 17Gel scooper ................................................................. 76GELase™ LMP Agarose Gel-Digesting Preparation .................. 17Gene Expression Analysis ............................................. 33-35Gene synthesis ........................................................... 131GenMap Human BAC Collection ......................................... 28Genome walking .......................................................... 151Genomic Cloning ....................................................... 18-21Genomic DNA, human, sonicated ................................ 149-150Genomic walking .................................................... 132,151GFP-anti, antibody ..................................................... 113GGTase-I ................................................................... 100GGTase-II .................................................................. 100Gloves ........................................................................ 75Glucagon-like peptides ................................................. 130Glucocorticoid receptor ............................................... 123Glutathione S-transferase ............................................. 126Glycogen synthase, substrate for CamK II ......................... 109Glycogen synthase, substrate for PKC .............................. 109Glycosidase assay- substrates .......................................... 88Glycosylase, HK™-UNG Uracil-DNA ..................................... 41Glycosylases & Excision Mixes .......................................... 41Glycosylases ................................................................. 41GNDF, Glial-derived neurotrophic factor .......................... 125GPCRs (G-protein coupled receptors) ........................ 100-103G-protein coupled receptors- cell extract ........................ 103G-protein ................................................................... 99Gram Positive DNA Purification Kit, MasterPure™ ................. 2growth factors ........................................................... 125Growth Hormone releasing factor ................................... 130Growth Hormone ........................................................ 125Growth Hormone-20K ................................................... 125GST ......................................................................... 126GST-anti, antibody ...................................................... 113

INDEX

iii


GTP (Guanosine-5'-Triphosphate) ..................................... 32GTP Family ............................................................ 96-103GTP Solution ................................................................ 32GTP ........................................................................... 32GTPase Cycle Effectors ............................................ 99-100GTPase modiying enzyme .............................................. 100Guanine Nucleotide Exchange Factor .................................. 99Gustducin ................................................................... 99

HH1R (HistamineH1 receptor)+regulator of G-protein signaling 102H1R (HistamineH1 receptor) .......................................... 102H1R-G protein fusion protein ......................................... 102H2 R(Histamine H2 receptor) ......................................... 101H2R (HistamineH2 receptor)+regulator of

G-protein signaling ................................................ 102H2R-G protein fusion protein ......................................... 102HA-anti, antibody ....................................................... 113Halogen containing nucleotides ......................................... 80Halogen-containing ATP analogs ......................................... 80Halogen-containing GTP analogs ........................................ 80HA-Tagged Yeast Strains ................................................. 27HBTU ....................................................................... 128Hck SH3 domain .......................................................... 106HCV Protease assay kits86HCV Protease- substrates ............................................... 86heavy atom derivatization -crystallography ........................ 80Hela cell cytosol extract ................................................ 123Hela cell nuclear extracts .............................................. 123Hepatitis A proteins ..................................................... 117Hepatitis B proteins .................................................... 117Hepatitis C proteins ............................................... 117-119Hepatitis C-related peptides ......................................... 130Hepatitis D proteins .................................................... 119Hepatits E proteins ..................................................... 119Herpes proteins .......................................................... 117HEX ......................................................................... 135HGF, Hepatocyte Growth factor ..................................... 125High Fidelity Polymerase .................................................. 9High Range DNA Ladder .................................................. 11Hilyte Fluor ................................................................ 135HisTag-anti, antibody .................................................. 113Histamine receptors .................................................... 102HIV Protease - substrates ............................................... 86HIV Protease assay kits .................................................. 86HIV related peptides ................................................... 130HIV Reverse Transcriptase .............................................. 15HIV-1 proteins ...................................................... 119,120HIV-2 proteins ........................................................... 120HK™ Thermolabile Phosphatase ........................................ 42HK-UNG™ Thermolabile Uracil N-Glycosylase ........................ 41HMG1, High mobility group .......................................... 1122HMP linker ................................................................ 128HOAt ....................................................................... 128HOBt ....................................................................... 128Hoechst dyes .............................................................. 137Holoenzyme, E coli RNA Polymerase, (sigma-saturated) .......... 29Homogenizer ................................................................ 67Hot Start Polymerase ...................................................... 9H-Ras ......................................................................... 96Human ORF Collection .................................................... 25Human T-cell Lymphotropic proteins (HTLV) ...................... 120Hybridase™ Thermostable RNase H ................................... 39Hybridization bottles ..................................................... 72Hybridization Oven ................................................... 72-73Hydrogen Peroxidase assay kit ......................................... 91HyperMu™ <CHL-1> Forward Primer .................................. 55HyperMu™ <CHL-1> Insertion Kit ....................................... 55HyperMu™ <CHL-1> Reverse Primer .................................. 55HyperMu™ <CHL-1> Transposon ........................................ 55HyperMu™ <KAN-1> Insertion kit ...................................... 55HyperMu™ <KAN-1> Transposon ........................................ 55HyperMu™ <R6Kγori/KAN-1> Tnp Transposome™ Kit .............. 52HyperMu™ <R6Kγori/KAN-1> Transposon ............................. 52HyperMu™ Transposase .................................................. 50

IIBI Base Runner 100 & 200 sequencers ............................... 62IBI MP1015 Electrophoresis System ................................... 60IBI STS-45I Sequencer .................................................... 62IBI Ultra-wide mini gel submarine system ........................... 60IBI Universal Protein Gel systems ...................................... 61IFN-α, 2b .................................................................. 124IFN-α-1b ................................................................... 124IFN-α-2a ................................................................... 124

IFN-β ........................................................................................................ 124IFN-γ ........................................................................................................ 124IL-1 receptor antagonist ............................................... 125IL-10 ........................................................................ 124IL-11 ........................................................................ 124IL-15 ........................................................................ 125IL-1a ........................................................................ 125IL-1b ....................................................................... 125IL-2 ......................................................................... 125IL-3 ......................................................................... 124IL-4 ......................................................................... 125IL-5 ......................................................................... 125IL-6 ......................................................................... 125IL-7 ......................................................................... 125IL-9 ......................................................................... 125IMAGE ESTs ................................................................. 25Immobilized 2',3'- EDA Nucleotides .................................... 58Immobilized 8-Aminobutyl Nucleotides ............................... 58Immobilized 8-Aminohexyl Nucleotides ............................... 58Immobilized Tyrosines .................................................... 58Importin ................................................................... 103Incyte Gene Collection .................................................... 25Indo-1 ...................................................................... 143Indo-5F .................................................................... 143Induction Solution, CopyControl™ .................................. 18,19Induction Solution, CopyCutter™ ....................................... 24Inhibitor of DNA Cleavage, TypeOne™ Restriction Inhibitor ..... 17Inhibitor of Transcription, Tagetin™ RNA Polymerase

Inhibitor ................................................................ 32Inhibitor Remover Resin .................................................. 2Inhibitors .......................................... (see relevant section)Inserting a Promoter ..................................................... 49Inserting an Origin of Replication ..................................... 49Insertion Kits ............................................................... 49Insulin like Growth factor binding protein ................... 125,126Insulin like Growth factor ........................................ 125,126Insulin ...................................................................... 124In-vitro Transcription ................................................ 29-32Isofreeze flipper temperature maintenance racks ................ 66

JJBS DNA Shuffling Mutagenesis Kit ..................................... 54JBS dNTP Mutagenesis Kit ............................................... 54JBS Error-Prone Random Mutagenesis Kit ........................... 54JBS Halo kits ................................................................ 80JBS Halo-ATP kits .......................................................... 80JBS Halo-GTP kits ......................................................... 80JBS Membrane Screen ................................................... 77JBS Methylation kit ....................................................... 79JBS Solubility kits ......................................................... 79JBScreen Basic ............................................................. 78JBScreen Buffer kits ..................................................... 78JBScreen Bulk kits ......................................................... 77JBScreen Cryo ............................................................. 82JBScreen Crystal Screening Kits ....................................... 77JBScreen Detergents ..................................................... 79JBScreen Heavy ............................................................ 80JBScreen HTS ............................................................... 77JBScreen Plus .............................................................. 79JBScreen Refill kits ....................................................... 77JBSolution Detergent test kit ........................................... 79JNK2a/SAPK1a (c-jun kinase/stress-activated protein kinase) 108JOE ......................................................................... 136

KKAN-2 FP-1 Forward Sequencing Primer ............................ 55KAN-2 RP-1 Reverse Sequencing Primer ............................. 55Kemptide, substrate for PKA ........................................ 108KGF-1, keratinocyte growth factor-1 .............................. 125KGF-2, keratinocyte growth factor-2 .............................. 125Klenow DNA Polymerase ................................................. 7,8KLH, maleimidyl .......................................................... 115Kool NC-45 RNAP Activity & Inhibitor Screening Kit ............... 30Kool NC-45 Universal RNA Polymerase Template .................... 30K-Ras ......................................................................... 96

LLabels ........................................................................ 75Ladders .................................................................. 11,12Lambda DNA (Bgl I) ....................................................... 11Lambda DNA/Bme (Ava II) ............................................... 11Lambda DNA/Bss T1I (StyI) ............................................. 11Lambda DNA/HindIII ...................................................... 11Lambda DNA ................................................................ 12Lambda Exonuclease ...................................................... 38Lambda GT10 Forward DNA Sequencing Primer .................... 13

INDEX

iv


Lambda GT10 Reverse DNA Sequencing Primer ..................... 13Lambda GT11 Forward DNA Sequencing Primer .................... 13Lambda GT11 Reverse DNA Sequencing Primer ..................... 13Lambda Terminase ........................................................ 38Lck SH3 domain .......................................................... 106Leptin, obesity factor ................................................. 124Leukemia Inhibitory factor ........................................... 124Leukotriene receptors ................................................. 102Leutenizing Hormone ................................................... 130LEXSY, Leishmania Expression System.......................... 153-154Library construction ................................................... 131Ligase, T4 DNA, Cloned .................................................. 43Lipophilic membrane tracers .................................... 145-148Liquid nitrogen dewars .................................................. 65Liver-X receptor ........................................................ 123Log Scale DNA Ladder .................................................... 12Long Range Polymerase .................................................... 9Low Range DNA Ladder ................................................... 11Luciferin ..................................................................... 92LY294002 ................................................................... 105Lysozyme .................................................................... 57

MM13 forward and reverse primers .................................... 13M13/pUC Forward DNA Sequencing Primer ......................... 13M13/pUC Reverse DNA Sequencing Primer .......................... 13Magnesium Chloride ....................................................... 12magnetic stirrer with hot plate ........................................ 68magnetic stirrer .......................................................... 68Malaria aspartyl Proteinase FRET substrate ........................ 87Mammalian Gene Collection .............................................. 25MAPK2 (mitogen activated kinase) .................................. 108MARKS Protein, substrate for PKC ................................. 109MasterAmp™ 10X PCR Enhancer ........................................ 32MasterAmp™ AmpliThem™ DNA Polymerase ........................... 8MasterAmp™ Buccal Swab Brushes - Remote Site Testing ......... 1MasterAmp™ Buccal Swab Brushes ..................................... 1MasterAmp™ Buccal Swab Kit - Remote Site Testing ............... 1MasterAmp™ Buccal Swab Kit ........................................... 1MasterAmp™ DNA Extraction Solution .................................. 1MasterAmp™ Extra-Long 2X PCR Premix 1 to Premix 9 ........... 6MasterAmp™ Extra-Long DNA Polymerase Mix ....................... 6MasterAmp™ Extra-Long PCR Kit ....................................... 6MasterAmp™ GREEN Real-Time RT-PCR Kit .......................... 10MasterAmp™ High Fidelity RT-PCR Kit ............................... 14MasterAmp™ PCR Optimization Kit with (NH4)2SO4 ............. 5-6MasterAmp™ PCR Optimization Kit without (NH4)2SO4 ......... 5-6MasterAmp™ RT-PCR Kit for High Sensitivity ....................... 15MasterAmp™ Taq DNA Polymerase Kit .............................. 7,8MasterAmp™ Taq DNA Polymerase .................................... 7,8MasterAmp™ Taq PCR Core Kit ......................................... 8MasterAmp™ Tfl DNA Polymerase Kit ................................ 7,8MasterAmp™ Tfl DNA Polymerase ..................................... 7,8MasterAmp™ Tth DNA Polymerase Kit ................................ 7,8MasterAmp™ Tth DNA Polymerase .................................... 7,8MasterPure™ Complete DNA & RNA Purification Kit ............... 3MasterPure™ DNA Purification Kit ..................................... 3MasterPure™ DNA Purification Kit for Blood ......................... 2MasterPure™ Gram Positive DNA Purification kit ................... 2MasterPure™ Plant Leaf DNA Purification Kit ........................ 2MasterPure™ Precipitation Solution for Blood ....................... 3MasterPure™ RNA Purification Kit ..................................... 3MasterPure™ Yeast DNA Purification Kit .............................. 4MasterPure™ Yeast RNA Purification Kit .............................. 4Matrix Metalloproteinases ........................................... 83-85Matrix Metalloproteinases-Substrates FRET based ............ 83-85MaxPlax™ Lambda Packaging Extract ................................. 21M-CSF ...................................................................... 124MEK1 ....................................................................... 106Membrane potential probes ...................................... 145-148Mesophilic DNA Polymerases ........................................... 7-9MessageMAX T7 Capped Message Transcription Kit ............... 31MessageMuter™ shRNAi Production Kit ............................... 37MgCI2, 25 mM .............................................................. 58MIA, Melanoma Inhibitory activity protein ........................ 126Microcentrifuge ........................................................... 64Microfuge ................................................................... 64Mid Range DNA Ladder ................................................... 11Minifridge .................................................................. 66Minifuge ..................................................................... 64MiniV In vitro Transcription kit ....................................... 30MKK6 ....................................................................... 106MMLV Reverse Transcriptase ........................................... 14MMP- antibodies .......................................................... 85MMP-1 Assay kits ...................................................... 83,84

Monoclonal antibody production ..................................... 133Monomethylated; m7G[5´]ppp[5´]G .................................. 19MonsterScript 1st-strand cDNA synthesis kit ....................... 14MonsterScript Reverse Transcriptase ................................ 14Mouse BAC Clones ......................................................... 28MPC Protein Precipitation Solution .................................... 3MPC Protein Precipitation Solution ................... 215M-Ras ........................................................................ 96mRNA Amplification ................................................... 33-35mRNA Purification .......................................................... 3mRNA Purification .......................................................... 3mRNA-ONLY Eukaryotic mRNA Isolation kit ............................ 3mRNA-ONLY Prokaryotic mRNA Isolation kit .......................... 3Mthylene blue ............................................................. 137Mu-End™Protein Truncation Kit ....................................... 53MUKAN-1 FP-1 Forward Sequencing Primer ......................... 52MUKAN-1 RP-1 Reverse Sequencing Primer ......................... 52Multidrug resistance assays ........................................... 133Mung Bean Nuclease ....................................................... 40Mutagenesis Kits ........................................................... 54Mutation Detection ........................................................ 43Myosin II, non muscle, regulatory ................................... 126Myricetin .................................................................. 105

NNatriuretic peptides ................................................... 130NBT/ROCK ................................................................ 113Needles and Loops for Inoculation ..................................... 76Nerokinins ................................................................. 130Nerve Growth factor ................................................... 126Neurogranin, substrate for PKC ..................................... 109Neuromedins .............................................................. 130Neuropeptide Y and analogs ........................................... 130Neurotensins and related peptides .................................. 130Neurotrophin3 ........................................................... 126Nickase ...................................................................... 41Nile Red .................................................................... 141Ninhydrin test kit ....................................................... 128Nitrocellulose membranes ................................................ 63Not I/KAN-3 FP-2 Forward Sequencing Primer .................... 54Not I/KAN-3 RP-2 Reverse Sequencing Primer ..................... 54N-Ras ......................................................................... 96NTP Mixture (ATP, CTP, GTP, UTP) ................................... 32NTPhos Thermolabile Phosphatase ..................................... 42Nuclear receptors ....................................................... 123Nuclease S1 .................................................................. 41Nylon membranes .......................................................... 63

OOil Red ..................................................................... 141Oligo dT ..................................................................... 13Oligo modifcation ......................................................... 13Oligo Synthesis ............................................................. 13OmniCleave™ Endonuclease .............................................. 40OPD ........................................................................... 91Optimization Kit ........................................................... 6Orexins .................................................................... 130Osteocalcins .............................................................. 130Owl adjustable Vertical system ........................................ 60Owl Bandit Mini Tank electroblotter .................................. 63Owl Bandit Tank Electroblotting sytems .............................. 63Owl Buffer Puffer Self ReCirculating Gel System .................. 59Owl Centipede Minigel System .......................................... 59Owl Gator Gel System .................................................... 59Owl Millipede Horizontal System ....................................... 60Owl Otter sequencing gel casting system ............................ 62Owl Panther Semi Dry Electroblotters ................................ 62Owl Penguin Dual-gel water-cooled electrophoresis system ...... 61Owl Puffin Single sided Minigel system ............................... 60Owl simple cast Minigel Systems ....................................... 59Owl Spider Electrophoresis System ................................... 59Owl Submarine Gel Electrophoresis equipment ..................... 59Owl T-Rex Aluminium Backed Sequencing System ................... 62Owl Whirlsystem Recirculating Gel system........................... 59Oxidase assay substrates ................................................ 92Oxytocins .................................................................. 130Ozma PEG-Series 48-Salt ................................................ 78

Pp300, tumor suppressor gene and transcription factor ........ 122p38a/SAPK2α ............................................................. 108p42 ......................................................................... 108p44 ......................................................................... 108p52, transcriptional coactivator .................................... 122p53, C-term deletion ................................................... 122p53, wild type ........................................................... 122

INDEX

v


p75, transcriptional coactivator .................................... 122p85α ........................................................................ 104Pancreatic polypeptides ............................................... 130Parathyroid hormone and related peptides ....................... 130pBR322 DNA ................................................................ 12PC (Phospatidylcholine) ................................................ 105PC4, positive cofactor 4 ............................................... 122pCC1BAC™/plndigoBAC-5 Forward Sequencing Primer ....... 19,20pCC1BAC™/plndigoBAC-5 Reverse Sequencing Primer ........ 19,20pCC1FOS™/pEpiFOS™-5 Forward Sequencing .................... 18,19pCC1FOS™/pEpiFOS™-5 Reverse Sequencing Primer .......... 18,19pCC2 Forward Sequencing Primer .................................... 18pCC2 Reverse Sequencing Primer ..................................... 18PCR CleanUp ................................................................ 17PCR Cloning ............................................................. 16,17PCR Enhancer ............................................................... 12PCR Optimization .......................................................... 6PCR ........................................................................ 5-17PDGFR (Platelet-derived growth factor receptor) .............. 106PE (Phosphatidylethanolamine) ....................................... 105Peptide synthesis ........................................................ 132Peptide YY and analog .................................................. 130Periplasmic protein extraction ........................................ 57PeriPreps™ Periplastic Protien Extraction Kit .................... 57peroxidase assay - substrates .......................................... 91Phage Lambda Extracts .................................................. 21Phage T7 DNA ............................................................... 12Phi29 DNA Polymerase, RepliPHI™ ...................................... 9Phi29 Polymerase Dilution Buffer, RepliPHI™ ......................... 9Phi29 Reagent Set, RepliPHI™ ............................................ 9Phosphatase assay kits ............................................ 111,112Phosphatase assay- substrate ........................................... 89Phospholipase assay- substrate ........................................ 89Phosphopeptides ........................................................ 130PI (Phosphatidylinositol) ............................................... 105PI3K antibodies .......................................................... 105PI3K inhibitors ........................................................... 105PI3K lipid kinase mix1 .................................................. 105PI3K lipid kinase mix2 .................................................. 105PI3Kα ....................................................................... 104PI3Kβ ....................................................................... 104PI3Kδ ....................................................................... 105PI3Kγ ....................................................................... 104PI-4,5P2 (Phosphatidylinositol bisphosphate) ..................... 105PI-4P ....................................................................... 105pIndigoBAC-5 (BamH I Cloning-Ready) ............................... 20pIndigoBAC-5 (Hind III Cloning-Ready) .............................. 20pIndigoBAC-5 Sequencing Primers .................................... 20Piperidine ................................................................ 128Pituitary adenylate cyclase activating peptides ................. 130Pituitary Growth hormone ............................................ 125PKA (Protein kinase A) ................................................. 106PKCα ....................................................................... 106Plasmid DNA library construction ................................... 132Plasmid prep ............................................................... 16PlasmidMAX Plasmid Isolation kit ...................................... 16Plasmid-Safe™ ATP-Dep DNase .......................................... 16Platelet derived growth factor ...................................... 126Platelet-activating factor receptors ................................ 103Pluronic F-127 ............................................................ 144pMOD™<MCS> Forward PCR Primer .................................. 51pMOD™<MCS> Forward Sequencing Primer ......................... 51pMOD™<MCS> Reverse PCR Primer ................................... 51pMOD™<MCS> Reverse Sequencing Primer .......................... 51pNPP ................................................................... 89, 113Poly(A) Tailing .............................................................. 31Polyclonal antibody production ...................................... 133Ponceau .................................................................... 141Power supplies ............................................................. 61Power supply for sequencing gel ....................................... 61PPAR, Peroxisome Proliferator-activated receptor ............ 123pPDM™ Sequencing Primer for pPDM™-1 & pPDM™-2 ........... 54pPDM™-1 Cloning Plasmid ............................................... 54pPDM™-2 Cloning Plasmid ................................................ 54Precipitation Solution for Blood ........................................ 3PreMix-Choice Kits ........................................................ 6Pre-mRNA splicing factors ............................................ 122Primed in situ synthesis .......................................... 149-150Primers Commonly Used for Sequencing ............................. 13Primers ...................................................................... 13Prion associated proteins ............................................. 116Prion cDNA ................................................................ 116Prion proteins ........................................................... 116PRL-3 ....................................................................... 111

Prolactin releasing peptides .......................................... 130Prolactin ................................................................... 124Propidium iodide ....................................................... 137Protease assay kits ....................................................... 87Protease assay- substrates .............................................. 87Proteases .................................................................... 87Protein kinase related peptides ..................................... 130Protein kinase substrate sampler kit ............................... 112Protein labeling kit, 5-FAM ............................................ 142Protein labeling kit, 5-ROX ........................................... 142Protein labeling kit, 5-TAMRA ........................................ 142Protein labeling kit, Alkalin phosphatase ........................... 142Protein labeling kit, AMCA-X ......................................... 142Protein labeling kit, APC ............................................... 142Protein labeling kit, Biotin ............................................ 142Protein labeling kit, Hilyte Fluor ..................................... 142Protein labeling kit, HRP .............................................. 142Protein labeling kit, Phycoerythrin ................................. 142Protein labeling kit, TR ................................................ 142Protein Molecular weight Marker ...................................... 12Protein Quantitation kit1 ................................................ 41Proteinase K ................................................................. 3Proteolipid proteins (PLPs) ........................................... 130PS (Phosphatidylserine) ............................................... 105PTEN ....................................................................... 111PTP-1B .................................................................... 111PTP-Basophil like ........................................................ 111PTPm ....................................................................... 111PTP-SL-catalytic domain .............................................. 111PTP-SL-ex ................................................................. 111PTP-SL-kim (kinase interacting motif) ............................. 111pUC 19 DNA ................................................................. 12pUC 19/MspI ................................................................ 11pUC19 DNA Control Template ........................................... 11PVDF Membranes .......................................................... 63pWEB::TNC™ Cosmid Cloning Kit ....................................... 21pWEB::TNC™ Cosmid Vector ............................................ 21pWEB::TNC™ Deletion Cosmid Transposition Kit ................... 21pWEB™ Cosmid Cloning Kit .............................................. 21Pyronin Y .................................................................. 137

QQ-Beta Replicase .......................................................... 29Quantitative PCR and RT-PCRQuenchers ............................................................ 139,140Quercetin ................................................................. 105QuickExtract™ DNA Extraction Solution ................................ 1QXL .................................................................... 139,140

RR6KAN-2 Reverse Primer ................................................ 52Rab GGTase ............................................................... 100Rab17 ........................................................................ 97Rab1A ........................................................................ 97Rab1B ........................................................................ 97Rab2 .......................................................................... 97Rab24 ........................................................................ 97Rab27A ...................................................................... 97Rab3A ........................................................................ 97Rab4A ........................................................................ 97Rab6A ........................................................................ 97Rab7 .......................................................................... 97RabGDI ...................................................................... 97Rac1 .......................................................................... 98RAD51 ...................................................................... 122RafRBD ................................................................ 99-100RalGDS-RBD ................................................................. 99Ran ........................................................................... 98Random primers ........................................................... 13RanGAP ...................................................................... 99Rap1A ........................................................................ 96Rap1B ........................................................................ 96RAP30 ...................................................................... 121RAP74 ...................................................................... 121RapGAP ...................................................................... 99Ras ........................................................................... 96RasGAP ...................................................................... 99RB, retinoblastoma protein ........................................... 122rBst DNA Polymerase ...................................................... 7Ready-Lyse™ Lysozyme Solution ........................................ 57ReadyPreps™ Protein Preparation Kit ............................... 57Real-Time PCR ............................................................. 10Real-Time RT-PCR ......................................................... 15Rec A Protein ............................................................... 37

INDEX

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Rec J Exonuclease ......................................................... 39RecA Protein, E coli ....................................................... 37RecBCD Nuclease, Ecoli ................................................... 39Red Cell Lysis Solution ..................................................... 3Renin-substrate ............................................................ 87REP-1 ....................................................................... 100REP-2 ....................................................................... 100Replica plating device .................................................... 76RepliPHI™ Phi29 DNA Polymerase ........................................ 9Rescue Cloning of Insertion Mutations ................................ 50resins, 2-chlorotrityl ................................................... 128resins, AFC, AMC, pNA ................................................. 128resins, Fmoc-Rink amide .............................................. 128resins, HMP ............................................................... 128resins, MAPS ............................................................. 128resins, MBHA ............................................................. 128resins, merrifield ....................................................... 128resins, PAM ............................................................... 128resins, TentaGel ......................................................... 128resins, Wang .............................................................. 128resins ...................................................................... 128Resorufin .................................................................... 89Restriction enzymes .................................................. 44-48Retinoic acid receptor ................................................. 123RetroAmp™ RT DNA Polymerase ........................................ 15Retroviral vectors ......................................................... 36Reverse transcriptase .................................................... 14RhoA .......................................................................... 98Rhod-2 ................................................................ 143,144Rhod-5F ............................................................... 143,144Rhod-5N .............................................................. 143,144Rhodamine ................................................................ 136Rhotekin ............................................................... 99-100Ribonucleoside-5’-Triphosphate Solutions ........................... 32RiboScribe™ SP6 RNA Probe Synthesis Kit ........................... 30RiboScribe™ T3 RNA Probe Synthesis Kit ............................ 30RiboScribe™ T7 RNA Probe Synthesis Kit ............................ 30RiboShredder™ RNase Blend ............................................ 40RIN2-RA .................................................................... 100Rink amide linker ........................................................ 128RNA Cap Analog, Methylated ............................................ 31RNA Cap Analog, Trimethylated ........................................ 31RNA Cap Analog, Unmethylated ......................................... 31RNA Interference (RNAi) ............................................. 36-37RNA Labeling ............................................................ 33-35RNA pol II- hRPB10 ...................................................... 121RNA pol II- hRPB12 ...................................................... 121RNA pol II- hRPB5 ........................................................ 121RNA pol II- hRPB6 ........................................................ 121RNA pol II- hRPB9 ........................................................ 121RNA pol II .................................................................. 121RNA pol II-CTD ........................................................... 121RNA Polymerases ........................................................... 29RNA Probe Synthesis ...................................................... 30RNA Purification for Microarray ....................................... 4RNA Purification from Yeast ............................................. 4RNA Purification .......................................................... 3,4RNAi ...................................................................... 36-37RNase A ...................................................................... 39RNase A-Resistant RNA ................................................... 30RNase H, E coli ............................................................. 39RNase I, E coli .............................................................. 40RNase III, E coli ............................................................ 40RNase T1, Aspergillus oryzae ........................................... 40RNase TA ..................................................................... 41RNase-Free DNase I ....................................................... 38RNTP ......................................................................... 32rocker- 3D .................................................................. 69rocker ....................................................................... 68Rolling Circle Transcription ............................................. 32ROX ......................................................................... 136RT-PCR .................................................................. 14,15Rubella virus proteins .................................................. 120

SS100A, Calcium binding protein A ................................... 126SARS virus proteins ..................................................... 120SB202190 (p38 MAPK inhibitor) ...................................... 108SB203580 (p38 MAPK inhibitor) ...................................... 108SCF, Stem cell factor .................................................... 124Secretins .................................................................. 130Sequencing .............................................................. 55,56Sequencing Polymerase .................................................... 9Sequencing Primer-1/pWEB::TNC™ Cosmid Vector ............... 21

SequiTherm™ Cycle Sequencing Kit .................................... 56SequiTherm™ DNA Polymerase .......................................... 56SequiTherm™ EXCEL™ II DNA Sequencing Kit - LC

for 25-41 cm gels ..................................................... 56SequiTherm™ EXCEL™ II DNA Sequencing Kit - LC for 66 cm gels 56SequiTherm™ EXCEL™ II Long-Read™ DNA Sequencing Kit - ALF™ 56SequiTherm™ EXCEL™ II Manual Sequencing Kit ..................... 56SequiTherm™ Stop/Gel Loading Buffer (Automated) .............. 56SequiTherm™ Stop/Gel Loading Buffer (Manual) ................... 56SequiTherm™ Stop/Gel Loading Buffer for LI-COR® or NEN® .. 56serine/threonine kinase substrate sampler kit ................... 112Serine/Threonine kinases ......................................... 106-110shaker orbital .............................................................. 69shaker ........................................................................ 69Short-Hairpin RNA .................................................... 36-37SHP-1 (src homology 2 domain phosphatase) ...................... 111shRNAi controls ............................................................ 36shRNAi Libraries .......................................................... 36shRNAi transfection kits ................................................. 36Signal Transduction blocking peptides .............................. 130Single Stranded DNA Binding Protein ................................. 37Site directed mutageneis .............................................. 132Slides for Microarray .................................................... 76Slot-Blot Manifold systems .............................................. 63Slot-Blot Minifold systems ............................................... 63SoilMaster™ DNA Extraction Kit ......................................... 2SoilMaster™ Inhibitor Removal Resin ................................... 2SoilMaster™ Spin Columns ................................................. 2Solubility kits ............................................................... 79Somatostatins ............................................................ 130Sp1 ......................................................................... 122SP6 Promoter DNA Sequencing Primer ............................... 13SP6- Promoter Primer ................................................... 13SP6 R&DNA™ Polymerase ................................................. 29SP6 RNA Polymerase ...................................................... 29SP600125 (JNK inhibitor) .............................................. 108Sphingomyelin ............................................................ 105Spin ColumnsSpreader .................................................................... 76Src SH3 domain .......................................................... 106Src ......................................................................... 106Stains-all ................................................................... 137Staurosporine ............................................................ 105Stickleback cDNA Collection ............................................. 26stirrer ....................................................................... 68Streptavidin, alkaline phosphatase .................................. 115Streptavidin, fluorophore conjugated ............................. 115Streptavidin, HRP ....................................................... 115Streptavidin .............................................................. 115Subcloning ................................................................. 132Substance P and analogs ............................................... 130Substrate for Calcineurin .............................................. 110Substrate for casein kinase ........................................... 110substrate for cdk ...................................................... 1110Substrate for cdk ...................................................... 5110Substrate for MAPK .................................................... 110Substrate for p43 (CD2) ............................................... 110Substrate for p60-src .................................................. 110Substrate for PKCe ..................................................... 109Substrate for PKCm .................................................... 109Substrate for PKG ...................................................... 109Substrate peptide for AMPK ......................................... 108Substrate peptide for CHK ............................................ 108Substrate peptide for CK1 ............................................ 108Substrate peptide for CK2 ............................................ 108Substrate peptide for DYRK .......................................... 108Substrate peptide for GSK3 .......................................... 108Substrate peptide for LKB1 .......................................... 108Substrate peptide for PKA ............................................ 108Substrate peptide for PKC ............................................ 108Substrate peptide for src ............................................. 108Substrate peptides for Abl ............................................ 108Substrate peptides for Akt ........................................... 108Substrate peptides for Cdc-2 ........................................ 108Substrates for CaM Kinase II ......................................... 109Substrates for PTK ..................................................... 110Substrates for PTP ..................................................... 110Substrates for PTP-1B ................................................. 110Sulfoxide reductase, methionine ..................................... 126Supercoiled DNA Marker Set ............................................ 20Supercoiled DNA Marker Set ............................................ 20Superoxide dismutase .................................................. 126Swabs, CatchAll™ Sample Collection Swabs ............................ 1Syntide, substrate for CamKII ....................................... 109

INDEX

vii


TT3 Promoter DNA Sequencing Primer ................................. 13T3- Promoter primer .................................................... 13T3 RNA Polymerase ........................................................ 29T4 DNA Endonuclease V ................................................... 38T4 DNA Ligase, Cloned .................................................... 43T4 DNA Polymerase ......................................................... 9T4 Polynucleotide Kinase, Cloned ...................................... 43T4 RNA Ligase .............................................................. 43T7 DNA Polymerase, Unmodified ........................................ 9T7 Promoter DNA Sequencing Primer ................................. 13T7- Promoter primer .................................................... 13T7 R&DNA™ Polymerase .................................................. 29T7 RNA Polymerase ........................................................ 29TA Buffer 10X .............................................................. 12TACE-substrate ............................................................ 87Tagetin™ RNA Polymerase Inhibitor ................................... 32TAMRA ..................................................................... 136Taq DNA Polymerase, MasterAmp™ ..................................... 8TAQurate™ GREEN Real-Time PCR MasterMix ....................... 10TAQurate™ PROBES Real-Time PCR MasterMix ..................... 10TargetAmp 1-Round amplification kit 103 ...................... 33-35TargetAmp 1-Round Aminoallyl-aRNA amplification kit 101 .. 33-35TargetAmp 2-Round Aminoallyl-aRNA amplificcation kit 10 .. 33-35TargetAmp 2-Round Aminoallyl-aRNA amplificcation kit 20 .. 33-35TATA box binding protein .............................................. 121Taylor-Wharton Cryogenic Refrigerators ............................ 65TBTU ....................................................................... 128TC-21/R-Ras 3 ............................................................. 96TC21-R-Ras 2 ............................................................... 96TE Buffer ..................................................................... 3Terminator 5'- Phosphate-Dependent Exonuclease ................. 40TET ......................................................................... 137TET-1 FP-1 Forward Primer ............................................ 55TET-1 RP-1 Reverse Primer ............................................. 55TFIIA ....................................................................... 121TFIIA-p12 .................................................................. 121TFIIA-p55 .................................................................. 121TFIIB ....................................................................... 121TFIID ....................................................................... 121TFIIE ........................................................................ 121TFIIEα, p56 ............................................................... 121TFIIEβ, p34 ................................................................ 121TFIIF, RAP74 .............................................................. 121TFIIF ........................................................................ 121TFIIH ....................................................................... 121TFIIH-p62 .................................................................. 121Tfl DNA Polymerase, MasterAmp™ ...................................... 8Thermolabile Phosphatases .............................................. 42Thermophilic DNA Polymerases ........................................ 7,8Thermus Thermostable RNA Polymerase .............................. 29Thiazole orange .......................................................... 137Thrombin related peptides ........................................... 130Thrombopoetin, megakaryocyte colony stimulating factor .... 124Thyroid hormone receptor ............................................ 123Thyrotrophin releasing hormones and related peptides ........ 130Tick-borne Encephalitis protein ..................................... 120Tissue & Cell Lysis Solution ............................................... 3tissue tearor ............................................................... 67Titin ........................................................................ 126TMB .......................................................................... 91TN FP-1 Forward Primer ............................................ 49,50TN RP-1 Reverse Primer ............................................ 49,50TNF peptides ............................................................. 130TNF-α ...................................................................... 124TNF-β ....................................................................... 124Tobacco Acid Pyrophosphatase ......................................... 42Topoisomerase I, core domain ........................................ 122Topoisomerase I, C-term domain .................................... 122Topoisomerase I, N-term domain .................................... 122Topoisomerase I .......................................................... 122Tough tags, spots .......................................................... 75Toxins ...................................................................... 130Toxoplasma proteins .................................................... 120Trancription factors, activators .................................... 121Transcription Buffer Package .......................................... 31Transcription factors, coactivators ................................ 122Transcription factors ............................................. 121-123

Transformation & Storage Solution .................................... 17Transformation ........................................................ 17,23TransforMax™ EC100D™ pir+ Electrocompent E coli ........... 23,24TransforMax™ EC100D™ pir-116 Electrocompent E coli ....... 23,24TransforMax™ EC100™ Chemically Competent E coli .......... 23,24TransforMax™ EC100™ Electrocompetent E coli ................. 23,24TransforMax™ EC100™-T1R Chemically

Competent E coli ................................................. 23,24TransforMax™ EC100™-T1R Electrocompetent E coli ........... 23,24TransforMax™ EPI300™ Chemically Competent E coli ......... 23,24TransforMax™ EPI300™ Electrocompetent E coli ................ 23,24TransforMax™ EPI300™-T1R Chemically Competent E coli ... 23,24TransforMax™ EPI300™-T1R Electrocompetent E coli .......... 23,24Transilluminators .......................................................... 74Transposome kits .......................................................... 52Transposon Construction Vector ....................................... 51Transposon Construction Vectors ...................................... 51Transposons ............................................................. 49-55Treponema proteins .................................................... 120Trimethylated; m2,2,7G[5´]ppp[5´]G ............................... 31TRITC ...................................................................... 137Trypsin-substrate ......................................................... 87TypeOne™ Restriction Inhibitor ........................................ 17Tyrosine Hydroxylase, substrate for MAPK ....................... 110Tyrosine kinases ......................................................... 106Tyrosine like kinases .................................................... 106Tyrosine protein kinase substrate sampler kit ................... 112

UUniversal Primers ......................................................... 13Unmethylated; G[5´]ppp[5´]G ......................................... 31Uracil N-Glycosylase , HK™-UNG ........................................ 41Uracil-DNA Excision Mix .................................................. 41Urea peroxide tablets .................................................. 113UTP (Uridine-5'-Triphosphate) ......................................... 32UTP (Uridine-5'-Triphosphate) ......................................... 32UV Crosslinker ............................................................. 74UV Lamp- Handheld ....................................................... 74UV shield/goggles .......................................................... 76UV-White ligh plate converter .......................................... 74

VVaccinia DNA Topoisomerase I .......................................... 37Vasoactive Intestinal peptides ........................................ 130Vasopressins .............................................................. 130VEGF, Vascular Endothelial growth factor ......................... 126Ventricular Myosin regulatory light chain .......................... 126VH1-related phosphatase .............................................. 111Vortex mixer ............................................................... 68VP16, Herpes simplex virion trans-activating protein ......... 121Varizella-Zoster Virus proteins ...................................... 120

WWASP-121 ................................................................. 100water bath .................................................................. 70Water, Sterile Nuclease-Free ........................................... 12Water ........................................................................ 12waterbath- shaking ....................................................... 70waterbath-circulating .................................................... 71WaterMaster DNA Purification kit ...................................... 2Wizard I and II ............................................................. 78Wortmannin .............................................................. 105WT-1(+KTS), Wilm’s tumor suppressor and

transcription splicing factor .................................... 122WT-1(-KTS), Wilm’s tumor suppressor andtranscription splicing factor ......................................... 122

XXenopus IMAGE cDNA ..................................................... 26X-Rhod-1 ............................................................. 143,144X-Y FISH Bovine sex test kit ...................................... 149-150

YYeast Insertional Mutant Strains ....................................... 27Yeast Knockout Strains ................................................... 27Yeast ORF Collection ...................................................... 27Yeast TAP Fusion Collection .............................................. 28Yeast Tet-promoter Hughes Strains .................................... 28

ZZebrafish Gene Collection ............................................... 26Zebrafish Morpholino Collection ....................................... 26

INDEX

viii

Documents

Biotechnology Biotechdesk Catalog 2006 07