BIOTECHNOLOGY BIOENGINEERING - Wiley Online Library...222 Modulation of Viability of Live Cells by Focused Ion-Beam ExposureT. Shinada, T. Akimoto, Y. Zhu, H. Goke, and I. Ohdomari

Volume 108, Number 1, January, 2011

FEATURES

vi Spotlight

ARTICLES

Biocatalysis, Protein Engineering, and Nanobiotechnology

1 Re-Structuring Protein Crystals Porosity for Biotemplating by Chemical Modifi cation of Lysine ResiduesN. Cohen-Hadar, S. Lagziel-Simis, Y. Wine, F. Frolow, and A. Freeman

Published online 7 September 2010

Biofuels and Environmental Biotechnology

12 Comparative Lipidomic Profi ling of Xylose-Metabolizing S. cerevisiae and Its Parental Strain in Different Media Reveals Correlations Between Membrane Lipids and Fermentation CapacityJ. Xia, A. D. Jones, M. W. Lau, Y.-J. Yuan, B. E. Dale, and V. Balan

Published online 27 August 2010

22 Increasing Cellulose Accessibility Is More Important Than Removing Lignin: A Comparison of Cellulose Solvent-Based Lignocellulose Fractionation and Soaking in Aqueous AmmoniaJ. A. Rollin, Z. Zhu, N. Sathitsuksanoh, and Y.-H. P. Zhang


Bioprocess Engineering and Supporting Technologies

31 Enhanced Butyric Acid Tolerance and Bioproduction by Clostridium tyrobutyricum Immobilized in a Fibrous Bed BioreactorL. Jiang, J. Wang, S. Liang, J. Cai, Z. Xu, P. Cen, S. Yang, and S. Li

Published online 21 October 2010

41 Perfusion of 3D Encapsulated Hepatocytes—A Synergistic Effect Enhancing Long-Term Functionality in BioreactorsR.M. Tostões, S. B. Leite, J.P. Miranda, M. Sousa, D.I.C. Wang, M. J.T. Carrondo, and P.M. Alves


BIOTECHNOLOGYBIOENGINEERING

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BIT-108(1)-CNT.indd 1BIT-108(1)-CNT.indd 1 11/23/10 11:45:15 AM11/23/10 11:45:15 AM

Bioseparations and Downstream Processing

50 Effects of Solution Environment on Mammalian Cell Fermentation Broth Properties: Enhanced Impurity Removal and Clarifi cation PerformanceM. Westoby, J. Chrostowski, P. de Vilmorin, J.P. Smelko, and J.K. Romero


59 Improved Process Analytical Technology for Protein A Chromatography Using Predictive Principal Component Analysis ToolsY. Hou, C. Jiang, A.A. Shukla, and S.M. Cramer

Published online 29 July 2010

69 Application of an Aqueous Two-Phase Systems High-Throughput Screening Method to Evaluate mAb HCP SeparationS.A. Oelmeier, F. Dismer, and J. Hubbuch


Cellular and Metabolic Engineering

82 Metabolic Flux Analysis of CHO Cell Metabolism in the Late Non-Growth PhaseN. Sengupta, S.T. Rose, and J.A. Morgan

Published online 29 July 2010

93 Metabolic Engineering of Escherichia coli for the Production of Cadaverine: A Five Carbon DiamineZ.-G. Qian, X.-X. Xia, and S.Y. Lee


Engineering Science of Biological Systems

104 Reaction–Diffusion Constraints in Living Tissue: Effectiveness Factors in Skeletal Muscle DesignS.K. Dasika, S.T. Kinsey, and B.R. Locke


116 Micropatterning and Characterization of Electrospun Poly(�-Caprolactone)/ Gelatin Nanofi ber Tissue Scaffolds by Femtosecond Laser Ablation for Tissue Engineering ApplicationsY.C. Lim, J. Johnson, Z. Fei, Y. Wu, D.F. Farson, J.J. Lannutti, H. W. Choi, and L.J. Lee


Systems Biotechnology

127 Cybernetic Models Based on Lumped Elementary Modes Accurately Predict Strain-Specifi c Metabolic FunctionH.-S. Song and D. Ramkrishna


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Tissue Engineering and Delivery Systems

141 Primary Rat Hepatocyte Culture on 3D Nanofi brous Polymer Scaffolds for Toxicology and Pharmaceutical ResearchJ. Bierwolf, M. Lutgehetmann, K. Feng, J. Erbes, S. Deichmann, E. Toronyi, C. Stieglitz, B. Nashan, P.X. Ma, and J.M. Pollok


151 Aggregation of Bovine Anterior Cruciate Ligament Fibroblasts or Marrow Stromal Cells Promotes Aggrecan ProductionJ.J. Lim, L.Scott Jr., and J.S. Temenoff


163 High-Throughput Screening of a Small Molecule Library for Promoters and Inhibitors of Mesenchymal Stem Cell Osteogenic DifferentiationD.M. Brey, N.A. Motlekar, S.L. Diamond, R.L. Mauck, J.P. Garino, and J.A. Burdick


175 Microfl uidic Synthesis of Composite Cross-Gradient Materials for Investigating Cell–Biomaterial InteractionsJ. He, Y. Du, Y. Guo, M. J. Hancock, B. Wang, H. Shin, J. Wu, D. Li, and A. Khademhosseini


186 CHO Cell Expression, Long-Term Stability, and Primate Pharmacokinetics and Brain Uptake of an IgG–Paroxonase-1 Fusion ProteinR.J. Boado, E.K.-W. Hui, J.Z. Lu, and W.M. Pardridge


197 Characterization of Protein Release From Hydrolytically Degradable Poly(Ethylene Glycol) HydrogelsS.P. Zustiak and J.B. Leach


207 Composite Thin Film and Electrospun Biomaterials for Urologic Tissue ReconstructionA.K. Kundu, J. Gelman, and D.R. Tyson


COMMUNICATIONS TO THE EDITOR

Bioprocess Engineering and Supporting Technologies

216 Quantitative Infl uence of Endogenous Salicylic Acid Level on Taxuyunna-nine C Biosynthesis in Suspension Cultures of Taxus chinensisX. Zhou and J.-J. Zhong


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222 Modulation of Viability of Live Cells by Focused Ion-Beam ExposureT. Shinada, T. Akimoto, Y. Zhu, H. Goke, and I. Ohdomari


Cellular and Metabolic Engineering

226 Bacillus subtilis pgsE (Formerly ywtC) Stimulates Poly-�-Glutamate Production in the Presence of ZincD. Yamashiro, M. Yoshioka, and M. Ashiuchi


Engineering Science of Biological Systems

231 Antimicrobial Testing for Surface-Immobilized Agents With a Surface-Separated Live–Dead Staining MethodJ.-B.D. Green, S. Bickner, P.W. Carter, T. Fulghum, M. Luebke, M.A. Nordhaus, and S. Strathmann


237 Detection and Reduction of Microaggregates in Insulin PreparationsC.L. Heldt, M. Sorci, D. Posada, A. Hirsa, and G. Belfort



Cover LegendFluid fi eld distribution of bioreactors with helical impeller using CFD software. See related article by Bao et. al., Biotechnology and Bioengineering, Volume 105, Number 4 (March 1, 2010), pages 718-728. (Image courtesy of Prof. Jie Bao et. al.)

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BIOTECHNOLOGY BIOENGINEERING - Wiley Online Library...222 Modulation of Viability of Live Cells by Focused Ion-Beam ExposureT. Shinada, T. Akimoto, Y. Zhu, H. Goke, and I. Ohdomari