BioPrint printed electrochemical sensor

development platform Marja Nissinen, Oulu University of Applied Sciences

Introduction to biosensors • Definitions and principles of

biosensors and electrochemical detection

• Advantages of printed biosensors

• Applications BioPrint facilities Production of electrochemical biosensors

Outlines

Biosensor Definition: A biosensor is a device that uses specific biochemical reactions to detect chemical compounds (analyte) in biological samples and transduces the reaction to the signals which are proportional to the amount of the analyte and can be measured.

Sample

Sample • Body fluids • Food sample • Industrial

process product

• Environmental field sample

Electrochemical transduction

Is based on movement of electrons (e-) in redox reactions detected when a potential is applied between two electrodes. The redox reactions in biosensors are often catalyzed by enzymatic reactions.

O2 H2O2 OH- e- e-

O2 redox reaction:

Glucose biosensor: excample of enzymatic recognition reaction and transduction of it to electrochemical signal

Printed technology

Screen printed electrode • Silver/AgCl2 reference

eletrode, RE • Carbon working electrode, WE • Carbon counter elctrode, CE • Dieletric insulating layer

Electrochemical cell • Electrodes • Wires connecting

electrodes and potentiostat

• Electrolyte and sample

Electrochemistry in analytics

Advantages of printed electrochemical biosensors

• inexpensive • point-of-care or on-site-test

• easy to perform, results available on site allowing fast decision making • reduce the number of unnecessary laboratory

tests • prescreening of samples for lab tests • result seen in numbers (not colours) • disposable

Applications of printed electrochemical biosensors • diagnostics glucose, cholesterol, urea, lactate, cardiac markers, drugs…

• environment toxicity of environment

• food processing bacteria, toxicity

• wellness lactate, stress

• industrial processes process products, enzymes, yeast

BioPrint-project

• Focuses on the development of electrochemical biosensors using printed technology

• Small scale pilot manufacturing laboratory for electrochemical biosensors is located at the Oulu University of Applied Sciences (OUS) • Equipments : Small scale R2R with Flexo, Gravure and Hot embossing units

• Screen printing machine • Inkjet • Dispenser

• Production of prototypes of electrochemical biosensors for research and development

• Development of small scale manufacturing processes for biosensors

• Set up network of companies and research organizations which are needed for the production of the biosensors

BioPrint-project

Focuses on

Fabrication of enzymatic screen printed biosensors

Each ink has its own layer.

Graphite working electrode

Mediator

Glutaraldehyde

Bioinks (enzymes)

Printing of electrodes Addition of the bioinks

Testing of biosensors: quality control, functionality,

reproducibility

Materials and printing • Conductivity • Light microscopy • Surface profiles • Scanning electron microscopy

Functionality • Electrochemical methods

• Cylic voltametry • Amperometry

Cyclic voltametry

SEM pictures

Surface profile

Electrochemical biosensor application

Sensor

technology

Sensor manufactur

ing

Sensor testing

Material development:

bioinks, conducting

inks

Materials: Biomolecul

es, inks, substrates

Instrumentation for signal

detection and

processing

Steps to the biosensor application prototype

BioPrint- printed electrochemical sensor development platform

Key questions for the application idea: What should be detected How to applicate the sample to the recognizing element? How to recognize the reaction? What is the signal from reaction and how to detect?

www.oamk.fi/bioprint www.oamk.fi/prinlab

Oulu University of Applied Sciences Antti Berg Harri Määttä Tomi Tuomaala Marja Nissinen

University of Oulu Mikko Nelo Jari Hannu Jari Juuti

VTT Liisa Kivimäki Leena Hakalahti