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Bacterial Effects of Bacterial Effects of Body Piercing Body Piercing Robert S. Bruneel Robert S. Bruneel Undergraduate Student Undergraduate Student Department of Education Department of Education Secondary Education Secondary Education Biology Biology Tennessee Technological University Tennessee Technological University Cookeville, TN 38501 Cookeville, TN 38501

Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

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Page 1: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Bacterial Effects of Bacterial Effects of Body PiercingBody Piercing

Robert S. BruneelRobert S. BruneelUndergraduate StudentUndergraduate Student

Department of EducationDepartment of EducationSecondary EducationSecondary Education

BiologyBiologyTennessee Technological UniversityTennessee Technological University

Cookeville, TN 38501Cookeville, TN 38501

Page 2: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Project SummaryProject Summary

PROBLEM/QUESTIONPROBLEM/QUESTION-Are body piercings more prone to bacterial formation, depending on the place of the piercing?

OBJECTIVEOBJECTIVE- - To prove that depending on the place of To prove that depending on the place of piercing, more bacteria formation is likely to occur around piercing, more bacteria formation is likely to occur around piercing site.piercing site.

HYPOTHESISHYPOTHESIS- - A person is more susceptible to A person is more susceptible to bacteria formation around piercing site.bacteria formation around piercing site.

NULL HYPOTHESISNULL HYPOTHESIS- - A person is not more A person is not more susceptible to bacteria formation around piercing site.susceptible to bacteria formation around piercing site.

Page 3: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Project SummaryProject Summary GOALGOAL::

– To prove that people that have body piercings are more prone to To prove that people that have body piercings are more prone to bacterial growth around the pierce site than someone without the bacterial growth around the pierce site than someone without the piercing at the same site.piercing at the same site.

HOW DO I ACCOMPLISH GOALHOW DO I ACCOMPLISH GOAL::– DataData: Swabs of people with different piercings.: Swabs of people with different piercings.

– TreatmentsTreatments: : 1. People with no piercing.1. People with no piercing. 2. People that have an ear piercing.2. People that have an ear piercing. 3. People with tongue piercing.3. People with tongue piercing.

– ControlControl: People with no piercings.: People with no piercings.

– ReplicatesReplicates: Two groups of people, both with ear piercings : Two groups of people, both with ear piercings and two groups of people with tongue piercings and two and two groups of people with tongue piercings and two groups of people with no piercings at all.groups of people with no piercings at all.

Page 4: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

IntoductionIntoduction

PROBLEMPROBLEM = = body piercing can cause body piercing can cause serious bacterial infections.serious bacterial infections.

HYPOTHESISHYPOTHESIS- - A person is more A person is more susceptible to bacteria formation around susceptible to bacteria formation around piercing site.piercing site.

NULL HYPOTHESISNULL HYPOTHESIS- - A person is not A person is not more susceptible to bacteria formation more susceptible to bacteria formation around piercing site.around piercing site.

Page 5: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Articles Being Articles Being ReferencedReferenced

The risks of ear piercing in childrenThe risks of ear piercing in children Schleroderma and body piercingSchleroderma and body piercing Monitoring and educational feedback to improve the Monitoring and educational feedback to improve the

compliance of tattooists and body piercers with infection compliance of tattooists and body piercers with infection control standards: A randomized controlled trialcontrol standards: A randomized controlled trial

Infection control practices among tattooists and body Infection control practices among tattooists and body piercers in Sydney, Australiapiercers in Sydney, Australia

Body piercing as a risk factor for viral hepatitis: An Body piercing as a risk factor for viral hepatitis: An integrative research reviewintegrative research review

Antibiotic prophylaxis, body piercing and infective Antibiotic prophylaxis, body piercing and infective endocarditisendocarditis

Body piercing: medical consequences and Body piercing: medical consequences and psychological motivationspsychological motivations

Mastitis Due to Mycobacterium abscessus after Body Mastitis Due to Mycobacterium abscessus after Body PiercingPiercing

Page 6: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Materials and MethodsMaterials and Methods Material/Supplies:Material/Supplies:

– SwabsSwabs– Petri dishesPetri dishes– IncubatorIncubator– PeoplePeople

Step-by-StepStep-by-Step: : 1. Obtain people.1. Obtain people. 2. Swab ear piercings.2. Swab ear piercings. 3. Swab tongue piercings.3. Swab tongue piercings. 4. Swab similar area of people with no piercings.4. Swab similar area of people with no piercings. 5. Streak Petri dishes and then incubate.5. Streak Petri dishes and then incubate. 6. Check for growth.6. Check for growth. 7. Record data and analyze.7. Record data and analyze.

Page 7: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Expected Results and Expected Results and BenefitsBenefits

Data SummarizationData Summarization: : Data will be summarized Data will be summarized by a chart of data including colony counts. by a chart of data including colony counts.

AssumptionsAssumptions: : The people that I do the experiment The people that I do the experiment on will have good hygiene and no colds.on will have good hygiene and no colds.

Expected ResultsExpected Results: : The people that I take swabs The people that I take swabs from will have more bacteria around pierce site, as others from will have more bacteria around pierce site, as others with no piercing will have less bacteria.with no piercing will have less bacteria.

Advantages/DisadvantagesAdvantages/Disadvantages = A lot of people = A lot of people have piercings, so it is easy to find subjects to experiment have piercings, so it is easy to find subjects to experiment on, but not everyone has the same hygiene so the on, but not everyone has the same hygiene so the experiment has some error.experiment has some error.

Page 8: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Bacterial Effects of Body Piercing Timeline

Time

Task Jan 17-24 Jan 24-31 Jan 31-Feb 7 Feb 7-14 Feb 14-21 Feb 21-28 Week 7

Research Topic/Develop Title              

Apply for Interlibrary Loan      

Develop Scientific Method      

Develop Objective/ Hypothesis        

Start Research Proposal        

Start Web Page      

Do Data Form    

Swab Pierce Sites TO BE DETERMINED

Collect Data/ Record Data TO BE DETERMINED

Page 9: Bacterial Effects of Body Piercing Robert S. Bruneel Undergraduate Student Department of Education Secondary Education Biology Tennessee Technological

Proposed Budget

Category    

Salaries & Wages First Year Second Year

Professional    

Technician $ 2,000 $ 2,000

Secretarial $ 1,000 $ 1,000

Graduate Student(2) $ 3,000 $ 3,000

Student Hourly $ 15 $ 15

Subtotal $ 6,015 $ 6,015

Benefits $ 500 $ 500

Travel $ 200 $ 200

Non-Expendables $ 300 $ 300

Expendables    

Computer $ 1,000 $ 1,000

Supplies & Expenses $ 500 $ 500

Copying & Telephone $ 300 $ 300

Tuition & Fees $ 2,000 $ 2,000

Subtotal $ 4,800 $ 4,800

Total Direct Costs $ 10,815 $ 10,815

Indirect Costs $ 500 $ 500

Total Costs $ 11,315 $ 11,315