HIGH SURVIVABILITY: Can withstand the harsh environment of stomach acid (low pH) and bile juices to reach the intestine where it colonizes

HIGHLY RESILIENT: Can survive the shipping, manufacturing, and transport processes without loss of any viability

SUPERIOR VIABILITY: Stable at room temperature, requires no refrigeration.

RESISTANT TO A GREATER RANGE OF TEMPERATURES: Can survive a range of temperatures - high boiling to low freezing temperatures. This has a wide application in the food industry as it can be incorporated into a broad range of foods

NEEDS NO MICRO ENCAPSULATION: Makes it a cost effective probiotic which can be incorporated into a wide range of foods

• Aids in digestion• Helps maintain the intestinal flora• Helps support a healthy immune system

Why We Recommend Applications

Bacillus coagulans Unique IS2 is an ideal ingredi-ent which can be added to foods, beverages and supplements to fortify them as it is stable across all temperatures. Some examples includeBaked foods - cakes , muffins and cookiesDry powders - cake and soup mixesFinished products -granola, protein bars and breakfast cereals Dairy products -cheese, milk products, ice creams and yoghurtsBeverages - coffee, tea and juicesCandies and gummies.

Food Applications

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Bacillus coagulans

Bacillus coagulans Unique IS2 is a gram positive, spore forming bacterium. It is non-pathogenic and safe for human consumption. Being naturally encapsulated, it is a robust and stable probiotic making it the probiotic of choice in the food and pharmaceutical industry.

Bulk powder 6/15/25/50/100/150/200/250 billion cfu/g and enteric coated pelletsFinished formulations - Capsules, Sachets & Chewable Tablets Fiber board drums with double polythene linersStore in a cool and dry place.

Availability, Packing & Storage

• Stable for 3 years

Stability

Supporting Documentation

• Certificate of Analysis (COA)• Material Safety Data Sheet (MSDS)• GMO Free Certificate• BSE/TSE Free Declarations• Stability Data • Dossier• Halal and Kosher Certification• WHO GMP, ISO 9001:2008

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References1. Ratna Sudha et al., (2010). Molecular typing and probiotic attributes of a new strain of Bacillus coagulans Unique IS-2 potential therapeutic agent ; Genetic Engineering and Biology journal. GEBJ-72. Ratna Sudha et al., (2011). Safety study of Bacillus coagulans Unique IS-2 in rats: morphological, biochemical and clinical evaluation; Interna-tional Journal of Probiotics and Prebiotics, 6,(1):43-483. Ratna Sudha et al., (2011). Clinical study of Bacillus coagulans Unique IS-2 in treatment of patients with bacterial vaginosis ; Indian Journal of Microbiology.52(3): 396-3994. Ratna Sudha et al., (2011). Effect of supplementation of Bacillus coagulans Unique IS-2(ATCC pat-11748) on hypercholesterolemic subjects: a clinical study; International Journal of Probiotics and Prebiotics, 6,(2):89-945. Sudha, Ratna M.; Bhonagiri, Shivaram (2012) Efficacy of bacillus coagulans strain Unique IS-2 in the treatment of patients with acute diarrhea; International Journal of Probiotics and Prebiotics: 7(1): 33

Specification For Bacillus coagulans

S.No

1.

2.

3.

4.

5.

6.

7.

8.

9.

Test

Description

Identification

Lactic Acid Producing Capacity

Loss on drying

Standard concentration

Pathogens

Yeast & Mold count

Other Organisms

Heavy metals:LeadCadmiumMercuryArsenic

Specifications

Free flowing white to grayish white to brown color powder with characteristic odour , slightly sweet in taste.

The spores are seen as small terminal oval shaped refractile bodies at the end of each vegetative cell.

Not less than 14 ml of 0.05 N Sodium hydroxide should be consumed

Not more than 5.0%

15/25/50/100/200 Billion cfu/g Should be absent

Not more than 100 cfu/g

Not more than 0.15 /0.25/0.5/1.0 / 2.0 million cfu/g Not more than 2 ppm Not more than 1 ppmNot more than 0.1 ppmNot more than 1 ppm