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cDNA 1356 267 5594
Dmy (DM-domain gene on the Y chromosome)
doublesex mab-3 DNA
SRY
DM-
DM-
- 2002 -
1 2 4 5 63
Exposure to Diethylstilbestrol During Embryonic and Larval Stages
Medaka Fish (Oryzias latipes) Leads to
Sex Reversal in Genetic Males and Reduced Gonad Weight
In Genetic Females
Bindhu Paul-Prasanth, Yasushi Shibata, Ryo Horiguchi, and Yoshitaka Nagahama
Endocrinology (in press)
SE
8 8-18 8-128
LE
18
28
18-28 18-128
LE
DES
DES
DES
0 6 8 18 12828
XY
XX
Cyp19a1
DMY
DES
Short exposure
Long exposure
0
400
600
XY XX
200
DES (ng/ml)
800
Co
nt
0.0
1
0.1
0
1.0
0
10
.00
10
0.0
0
Co
nt
0.0
1
0.1
0
1.0
0
10
.00
10
0.0
0
DES
0
200
300
XY XX
100
DES (ng/ml)
400
Co
nt
0.0
1
0.1
0
1.0
0
10
.00
10
0.0
0
Co
nt
0.0
1
0.1
0
1.0
0
10
.00
10
0.0
0
1 ng/ml
DES
Ge
rm c
ell
nu
mb
er
0
400
800
8 18 8 18
XY XX
Brd
U-p
osit
ive g
erm
cell n
um
ber
0
80
120
XY XX
40
8 18 8 18
XX 18 days after fertilization
Positive control Control DES
Effects of DES on germ cell apoptosis (TUNEL technology)
80 �m
XY XX
Effect�of�DES�on�gonado�somatic�index�(GSI)
Cont
XY
SE-
Washed
XY
LE-
Washed
XY
Cont
XX
SE-
Washed
XX
LE-
Washed
XX
0.00
2.00
4.00
6.00
Gon
ado-
som
atic
inde
x (G
SI)
XY XX
Cyp19a1 expression�after�withdrawal�of�DES
20000
16000
12000
8000
4000
0
Cont SE LE Cont SE LE
DES Cyp19a1
DES