At2g04040

MATE efflux

At1g02850

Glycosyl Hydrolase

At1g77120

ADH1

At2g43820

UDP glucoronosyl transferase

At1g26770

EXP10

KO-1 OX-1 Col-0 C24

Supplemental Figure 2. QRT- PCR analysis of selected genes up-regulated in ANAC102 over-expressing lines. Changes in expression were monitored for a set of genes in each of Col-0 (open circles), C24 (closed circles), KO-1 (open squares) and OX-1 (filled squares) at various points following exposure to 0.1% oxygen. Data presented are expression ratios at each time point for each Arabidopsis line relative to Col-0 expression at time-point 0 (± 1 Standard Error, n=9).

-1.5

8.5

18.5

28.5

38.5

48.5

58.5

68.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

nAt5g63790

ANAC102

-1.5

0.5

2.5

4.5

6.5

8.5

10.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-1.5

3.5

8.5

13.5

18.5

23.5

28.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-1.50.52.54.56.58.5

10.512.514.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-1.5

0.5

2.5

4.5

6.5

8.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-0.5

0.5

1.5

2.5

3.5

4.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

At1g72680

CAD

At2g30140

UDP glucoronosyl transferase

At1g68570

POT

At3g56710

Sig 1A binding protein

At5g43580

Protease inhibitor

KO-1 OX-1 Col-0 C24

Supplemental Figure 2. QRT- PCR analysis of selected genes up-regulated in ANAC102 over-expressing lines. Changes in expression were monitored for a set of genes in each of Col-0 (open circles), C24 (closed circles), KO-1 (open squares) and OX-1 (filled squares) at various points following exposure to 0.1% oxygen. Data presented are expression ratios at each time point for each Arabidopsis line relative to Col-0 expression at time-point 0 (± 1 Standard Error, n=9).

At3g59140

ABC transporter

-1.5

3.5

8.5

13.5

18.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

0

0.5

1

1.5

2

2.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

0.5

1.5

2.5

3.5

4.5

5.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-1.5

0.5

2.5

4.5

6.5

8.5

10.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-1.5

0.5

2.5

4.5

6.5

8.5

10.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n

-1.50.52.54.56.58.5

10.512.5

0 5 10 15 20 25

time (hours)

Rel

ativ

e ex

pre

ssio

n