ars.els-cdn.com · Web view0.0119 138387 5.36 3.4 3 36 c21499_g1_orf1 Lysosomal aspartic protease 0.625 0.0119 48256 8.73 33.9 9 559 c15173_g2_orf1 Fasciclin-like protein 0.6275 0.0179

Supplementary material

Comparative quantitative proteomics unveils putative mechanisms involved into

mercury toxicity and tolerance in Tigriopus japonicus under multigenerational

exposure scenario

Xiaoqun Xu c, Lin Shi a, Minghua Wang a, b, *

a Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems,

Xiamen University, Xiamen, 361102, China

b Center for Marine Environmental Chemistry and Toxicology/College of the

Environment & Ecology, Xiamen University, Xiamen, 361102, China

c Second Institute of Oceanography, State Oceanic Administration People's Republic

of China, Hangzhou, 310012, China

*Corresponding author at: Key Laboratory of the Ministry of Education for Coastal

and Wetland Ecosystems, Xiamen University, Xiamen, 361102, China

E-mail address: [email protected]

Phone: +86-592-2880219; Fax: +86-592-2880219.

.

Number of figures: 3

Number of tables: 10

Text S1: Biochemical parameter determination

For the biochemical assays, the F0 copepods were homogenized for 5 min in 0.85 %

saline at 4 ºC, using a glass-glass Elvehjem-Potter homogenizer. The homogenate was

centrifuged at 10000 g for 20 min at 4 ºC, and the supernatant was provided for

biochemical parameter determination using the commercial assay kits (Nanjing

Jiancheng Bioengineering Institute, Nanjing, China). Protein content determination

was performed according to the Bradford Coomassie brilliant blue method with

bovine serum albumin as a standard.

Chymotrypsin activity was determined by the colorimetric method, and the OD of

the supernatant was read in a spectrophotometer at 660 nm. All the experimental

samples were run by using a substrate free control and an enzyme free control. One

unit of chymotrypsin activity was calculated as 1 µg of tyrosine released per minute

per mg protein at 37 ºC.

Nitric oxide synthase produces nitric oxide (NO) by catalyzing the conversion of L-

arginine to L-citrulline. This assay was undertaken with the commercial kit by

measuring the concentration of the products NO with the absorbance at 530 nm. One

unit of nitric oxide synthase activity was calculated as 1 nmol of NO produced per

minute per mg protein at 37 ºC.

The levels of reduced glutathione (GSH) were measured according to the protocols

of the GSH assay kit. After reaction with 5,5-dithiobes-(2-ni-trobenzoic acid), the

GSH levels were determined at 405 nm with a spectrophotometer. The results are

expressed as the contents (μmol GSH) per g protein.

Malondialdehyde (MDA) level is used as a marker of lipid peroxidation. Prior to the

analysis of MDA, tissue samples were placed in 50 μL of 5 mM butylated

hydroxytoluene to prevent further lipid peroxidation. MDA level was assessed using

the commercially available colorimetric MDA-586 assay kit with the absorbance read

on a microplate reader at a wavelength of 586 nm. All experimental procedures for

these assays were performed using the instructions provided by the manufacturer.

MDA of the samples was normalized to protein content and expressed as μmol/g.

Figure captions

Fig. S1. The reproducibility was performed for the two biological replicates per

generation (i.e., F0, F2, and F5) via Pearson's correlation analysis, which was based

on the log ratios of the differentially expressed proteins. F03 and F04 were

representing the two replicates in the treated Tigriopus japonicus proteome, with F01

and F02 being the two ones in the control group, respectively. Consequently, the ratios

of F03/F01 and F04/F02 indicated the two biological replicates in F0. So did the F2

and F5.

Fig. S2. Comparison of the enzyme activity and protein expression for chymotrypsin-

like proteinase and nitric oxide synthase, respectively, in the F0 Tigriopus japonicus

under the control and 50 µg/L treatment. Data are described as means ± semi-range (n

= 2).

Fig. S3. The levels of reduced glutathione (GSH; A) and lipid peroxidation (LPO; B)

in the F0 Tigriopus japonicus under the control and 50 µg/L treatment. Data are

described as means ± semi-range (n = 2).

Fig. S1

Fig. S2

Fig. S3

Table S1 A summary for quantitative data in protein identity for the three Tigriopus

japonicus proteome sets via mass spectrometry analysis.

Group name

Total spectra

Spectra Unique spectra

Peptide Unique peptide

Protein FDR

Run1 199075 20141 15495 10080 8686 2827 0.01Run2 190112 18920 14381 9429 8038 2662 0.01

Run3 183923 24958 19644 14022 12292 3373 0.01

Table S2 The list of the up-regulated proteins for the copepod Tigriopus japonicus in F0.

Protein accessionProtein description F0 ratio P value MW [Da] pI AASC [%] MP Score

c17793_g1_orf1 Exo-beta-1,3-glucanase 2.642 0.0093 56050 4.46 13.7 5 194

c48715_g1 2.5495 0.0326 12339 4.64 13 1 24

c31704_g1_orf1 Trypsin type 4 (Fragment) 2.4895 0.00492 32797 4.53 11.1 2 280

c5952_g1 2.279 0.0124 21140 5.73 13.6 2 42

c62078_g1_orf1 Transporter 2.08 0.0477 80224 4.88 4.1 2 39

c20273_g1_orf1 Angiotensin-converting enzyme, putative 1.9865 0.0461 89375 4.5 8 2 37

TJ_CDS_13424-1 Carboxypeptidase B 1.926 0.0151 52426 4.72 10.6 2 108

TJ_CDS_10433-1 Sphingomyelin phosphodiesterase, putative 1.919 0.027 45659 4.68 5.6 2 52

c12760_g1_orf1 CD36 family protein 1.9115 0.0352 71791 4.48 5.1 2 58

c39594_g1_orf1 Putative xaa-pro aminopeptidase (Fragment) 1.9105 0.036 89110 5.38 8 5 124

c46825_g1_orf1 Chymotrypsin-like proteinase 1.871 0.038 33855 5.39 23.3 4 1118

c22200_g1_orf1 Cytochrome P450-like protein 3 (Fragment) 1.858 0.00668 71337 6.41 4.9 2 48

c16035_g1_orf1 Arginine kinase 1.855 0.0246 50450 6.08 44.3 12 610

c61653_g1_orf1 GABA-alpha subunit (Fragment) 1.833 0.0176 47757 6.09 6.3 1 49

c1269_g1_orf1 Putative endo-1,3(4)-beta-glucanase 1.8205 0.00349 35325 6.27 9.2 1 57

TJ_CDS_15294-2 Alcohol dehydrogenase zinc-binding domain protein 1.767 0.0207 45111 5.67 15.2 5 108

TJ_CDS_15251-1 Apolipoprotein D 1.754 0.0144 26987 4.46 4.9 1 26

c24400_g1_orf1 FI06514p (Fragment) 1.7485 0.0471 72182 4.4 15.8 5 279

TJ_CDS_11582-1 L-fucose isomerase and related proteins-like protein 1.703 0.0362 62500 6.12 4.5 1 69

TJ_CDS_09776-1 Aminopeptidase 1.668 0.0324 85512 4.98 3.4 2 40

c2851_g1_orf1 Kelch repeat-containing protein 1.653 0.0175 40471 4.8 11.1 2 107

TJ_CDS_22533-1 Transmembrane 9 superfamily member 3 1.646 0.0462 74680 5.94 2.1 1 75

c50370_g1_orf1 FG-GAP repeat family protein 1.6365 0.0125 55622 4.35 32.2 9 719

c15173_g1 1.6275 0.00862 24202 4.31 11.2 1 63

TJ_CDS_04924-1 Ferritin light chain, oocyte isoform 1.613 0.0363 29087 6.29 38.8 6 261

c39778_g1_orf1 Nicotinic acetylcholine receptor alpha-2 subunit 1.612 0.0135 35187 5.05 2.9 1 34

c61549_g1_orf1 Vitellogenin-like protein 1.6025 0.0143 198827 7.86 59.6 68 17824

c46869_g1_orf1 Aminopeptidase N 1.5835 0.0256 125758 4.59 8.1 7 197

c54917_g1_orf1 Hypothetical protein 1.5705 0.0117 56645 7.04 2.7 1 43

c8328_g1_orf1 Putative this conserved domain family includes a large number (Fragment) 1.5555 0.0178 71495 4.46 3.2 2 34

TJ_CDS_06891-1 Sodium potassium adenosine triphosphatase (Fragment) 1.5395 0.0324 27873 4.69 21.4 4 405

c20286_g1_orf1 Transporter 1.5355 0.0445 50441 6 8.7 2 80

c23394_g1_orf1 Urocanate hydratase 1.5125 0.0416 86206 5.74 10.1 3 83

c15358_g1 1.501 0.0495 32111 4.81 21.5 4 595

c3366_g1_orf1 AAEL005137-PA 1.4955 0.0353 35945 4.61 7.1 1 76

c23072_g1_orf1 Cytochrome P450 isoform 1-like protein (Fragment) 1.495 0.0373 71967 6.16 11.7 4 88

c21690_g1_orf1 Nitric oxide synthase 1.4945 0.0277 133843 6.25 3 3 53

c31445_g1_orf1 Hypothetical protein BRAFLDRAFT_75999 1.45 0.0226 55950 4.32 1.9 1 42

c62155_g1_orf1 Spermine synthase 1.444 0.0401 56037 4.89 6.4 2 50

TJ_CDS_05716-1 Sodium-independent sulfate anion transporter 1.431 0.0428 74892 8.09 3 2 45

TJ_CDS_04641-1 Glutamic acid decarboxylase 1, isoform D 1.4255 0.0262 66473 6.08 3.7 2 71

c55293_g1 1.4145 0.0376 25133 4.48 34.2 4 122

c23968_g1 1.406 0.0485 95603 5.49 8.2 5 153

TJ_CDS_19121-1 Hect E3 ubiquitin ligase, putative 1.3975 0.0408 327858 5.19 0.3 1 28

c3857_g1_orf1 Diaminopimelate decarboxylase 1.3955 0.000805 58727 5.87 4 1 42

c22725_g1_orf1 Short stop/Kakapo long isoform 1.3895 0.0449 626176 5.35 26.3 91 2773

c18174_g1_orf2 Chitin deacetylase 9 1.383 0.00665 51617 4.72 12.7 5 251

TJ_CDS_14183-2 Serine proteinase stubble 1.3735 0.00937 53918 5.2 16.2 4 137


TJ_CDS_09089-1 Cystathionine gamma-lyase 1.3695 0.0267 54827 6.45 25.7 8 299

c54164_g1_orf1 Aminoacylase-1 1.369 0.00173 60595 6.42 4.2 2 67

TJ_CDS_15261-2 Ras-related protein Rab-11A 1.362 0.00176 27172 5.98 7.9 2 36

c61606_g1 1.3585 0.0346 26164 4.27 9.8 2 124

TJ_CDS_02320-1 Calcineurin subunit B isoform 2 1.353 0.0234 23016 4.48 10.6 1 47

c17865_g1_orf1 Hydroxypyruvate isomerase 1.344 0.0277 35384 5.06 23.1 6 132

TJ_CDS_09128-1 3-hydroxyisobutyrate dehydrogenase 1.34 0.0131 47393 7.63 6.6 2 53

c23960_g1_orf1 Periostin 1.331 0.0269 41440 7.98 17.1 3 142

c31637_g1_orf1 Putative endo-1,3(4)-beta-glucanase 1.3305 0.0241 37589 7.11 3.2 1 44

TJ_CDS_02364-1 1.3195 0.0209 33574 4.81 3.9 1 30

c9826_g1 1.314 0.0486 14506 10.29 8 1 26

TJ_CDS_21797-2 V-type proton ATPase subunit S1 1.3035 0.0199 42975 6.6 6.7 2 46

TJ_CDS_08477-2 Twitchin (Fragment) 1.2985 0.0479 40523 6.03 4.2 1 26

TJ_CDS_18079-1 Putative smoothelin (Fragment) 1.292 0.0414 25523 8.75 15.2 2 66

c54486_g1_orf1 Low density lipoprotein receptor, putative 1.267 0.00238 211732 4.75 22.1 24 765

c14279_g2_orf2 Sorting and assembly machinery component 1.2475 0.0244 60698 6.5 9.7 3 63

c20934_g1_orf1 Putative carnitine o-acyltransferase crot (Fragment) 1.2465 0.0194 87475 7.44 3.2 2 36

c39359_g1_orf1 Endoplasmic reticulum-Golgi intermediate compartment protein 1 1.2445 0.0143 38659 6.88 8.6 2 107

c14502_g1_orf1 Tropomyosin 1.2425 0.00394 42851 4.89 17 4 117

c54525_g1_orf1 Phosphatidate cytidylyltransferase 1.2425 0.00131 56724 7.18 6.3 2 108

c39411_g1_orf1 Zgc:56585 protein 1.241 0.0343 40876 8.24 20.9 3 78

c13353_g1 1.2395 0.0173 66269 4.89 2 1 26

TJ_CDS_18232-1 Serine/threonine-protein phosphatase 2A catalytic subunit 1.2385 0.004 39786 5.29 32.4 7 211

c17352_g1_orf1 AAEL008862-PA 1.235 0.0298 139498 5.07 3.8 3 63

TJ_CDS_23612-1 Solute carrier family 2, facilitated glucose transporter member 1 1.233 0.00273 59006 5.26 4.9 1 85

c54151_g1_orf1 Putative Na/Ca exchanger 1.231 0.0138 106660 4.76 5.3 3 96

TJ_CDS_18957-1 Putative neurexin iv (Fragment) 1.2165 0.0191 164114 5.16 3.5 3 63

TJ_CDS_19957-1 Calcium-binding mitochondrial carrier protein Aralar2 1.215 0.0266 88221 6.96 14.2 6 258

TJ_CDS_00120-1 Protein 5NUC 1.2145 0.00742 75806 5.33 3.3 1 37

TJ_CDS_21811-1 Protein kinase 1.21 0.0152 43602 5.85 7.6 2 66

TJ_CDS_07292-1 AAEL009415-PA 1.209 0.0365 66821 5.18 2.4 1 25

c17322_g1_orf1 Putative aminopeptidase W07G4.4 1.2025 0.011 73051 8.09 16.9 7 248

c17276_g1_orf1 Mitochondrial glutamate carrier protein 1.2015 0.03 42263 9.7 6.3 2 46

F0 ratio: the average ratio for F03/F01 and F04/F02. MW: molecular weight. pI: isoelectric point. AASC: amino acid sequence coverage. MP: matched unique peptide.

Table S3 The list of the down-regulated proteins for the copepod Tigriopus japonicus in F0.

Protein accessionProtein description F0 ratio P value

MW

[Da]pI AASC [%] MP Score

c21457_g1_orf1 PREDICTED: uncharacterized protein LOC103305877 0.1445 0.0145 21061 5.03 11.5 2 92

c22761_g2 0.34850.00048

944508 9.26 8.3 2 94

c39303_g1 0.397 0.00528 49655 4.31 2 1 32

c17387_g1_orf1 SPT transcription factor family member 0.3995 0.00265 87095 7.65 36.4 19 551

c31508_g1_orf1 Polysaccharide deacetylase in N-acetylglucoamine utilization 0.4185 0.00493 38431 8.9 10.2 3 142

c8384_g1 0.43 0.0234 47492 8.14 13 4 170

TJ_CDS_20532-1 Chromobox protein homolog 1 0.4395 0.0391 23324 5.08 12.2 1 38

TJ_CDS_01066-1 60S ribosomal protein L17 0.459 0.0306 2767410.2

713.6 1 84

TJ_CDS_00774-2 Globin D, coelomic, putative 0.462 0.0425 27481 8.89 3.4 1 35

c8255_g1_orf1 Ribosomal protein L37 0.469 0.0144 1764111.5

212.8 2 41

c47444_g1 0.48 0.0233 60679 4.6 2.8 1 45

c2740_g1_orf1 Putative replication factor-a protein 1 0.4825 0.024 87564 6.49 1.8 1 27


c13669_g1_orf1 Cathepsin i (Fragment) 0.487 0.00869 44255 5.94 5.2 1 42


721.1 2 63

c19540_g1_orf1 Metastasis-associated protein MTA1 0.51 0.0441 94832 9.58 4.1 3 37

c2138_g1_orf1 Probable serine carboxypeptidase CPVL 0.5185 0.0139 64099 7.35 3 2 36

TJ_CDS_16977-3 Vitellogenin 2 0.5235 0.0447 237843 8.27 54 76 10065

c19007_g1_orf1 60S ribosomal protein L13 0.539 0.0235 3379111.5

511.5 2 45

c24318_g1_orf1 Cathepsin i (Fragment) 0.5395 0.0228 25449 4.75 15.4 2 83

c19400_g1_orf1 Caprin-1 0.5415 0.0229 94721 8.96 1.6 1 25

TJ_CDS_24307-1 Putative tubulin alpha-1 chain 0.5465 0.0358 55176 5.01 49.8 14 1207

c20430_g1_orf1 Vitellogenin 2 0.552 0.0284 238394 8.4 63.8 93 11420

c47068_g1_orf1 Survival of motor neuron-related-splicing factor 30 0.5535 0.0178 35621 9.5 8.7 2 84

TJ_CDS_18528-1 Proliferating cell nuclear antigen 0.563 0.00437 33337 4.63 13.1 1 59

c13485_g1_orf1 CG16799-like protein (Fragment) 0.5655 0.0388 28818 4.85 9.1 2 55

c17640_g1_orf1 Histone H1 0.5655 0.0125 3255110.7

322.2 3 66

c22969_g1_orf1 Multiple ankyrin repeats single kh domain protein (Fragment) 0.566 0.00587 245324 8.16 1.5 2 39

c22456_g1_orf1 DNA topoisomerase 2 0.571 0.0104 230448 8.79 0.6 1 39

TJ_CDS_12185-1 Putative ribosomal protein 49 0.578 0.0166 2455210.7

95.8 1 51

c46835_g1_orf1 Protein jagged-1 0.584 0.0352 50002 4.67 14.7 6 128

TJ_CDS_18188-1 60S ribosomal protein L10 0.5845 0.0466 30466 10 21.5 5 58

TJ_CDS_07457-1 Putative dna replication licensing factor mcm4 component (Fragment) 0.5905 0.0334 106483 5.24 2.5 2 35


c31579_g1_orf1 3-ketoacyl-CoA thiolase, mitochondrial 0.6055 0.0266 52498 8.2 12.8 4 138

c6985_g1_orf1 Eukaryotic translation initiation factor 4 gamma 2 0.607 0.0453 175223 8.42 1.6 1 34

c4422_g1_orf1 Selenophosphate synthetase 0.609 0.00488 52762 6.25 6.7 4 99

TJ_CDS_06925-1 N-alpha-acetyltransferase 40 0.615 0.00827 35721 6.48 9.5 2 61

c14127_g1_orf1 Piwi-like 1 protein (Fragment) 0.6265 0.0213 53373 9.05 8.3 3 91

TJ_CDS_12267-1 Myb-binding protein 1A 0.6285 0.0197 173213 5.72 1.9 2 54

c21806_g2_orf1 Vitellogenin 0.6295 0.0386 237746 8.74 63.2 95 9350

c19772_g1_orf1 Ribonucleoside-diphosphate reductase 0.6335 0.0477 110197 6.06 2.9 2 32

c8331_g1_orf1 Histone deacetylase 0.641 0.0266 60714 5.56 1.9 1 32

TJ_CDS_06955-1 Mitochondrial heat shock protein 60 0.6445 0.042 72164 5.38 16.6 7 269

c23945_g1_orf1 60S ribosomal protein L23a 0.6455 0.0206 3261410.7

327.1 3 164


730.2 4 70

c23818_g1_orf1 60S ribosomal protein L5 0.65 0.0382 43546 9.81 20.2 5 164

c61793_g1_orf1 JAB-MPN domain protein 0.6545 0.0101 46694 5.47 4.6 2 46

c47582_g1 0.657 0.00742 56195 4.37 6.4 2 45

c4648_g1_orf1 Peptidyl-prolyl cis-trans isomerase 0.6585 0.0326 23220 8.85 4.9 1 28

TJ_CDS_23122-2 NADH dehydrogenase iron-sulfur protein 8, mitochondrial 0.659 0.0447 27747 5.8 8.3 1 41

TJ_CDS_07843-1 GTP-binding nuclear protein Ran 0.666 0.0172 29375 6.97 17.7 2 63


TJ_CDS_13407-1 Probable pyridoxine biosynthesis SNZERR 0.667 0.00574 38175 5.83 13.2 2 73

TJ_CDS_18177-1 Eukaryotic translation initiation factor 5A 0.6685 0.0374 20318 4.89 7 1 64

c8400_g1_orf1 Tissue specific transplantation antigen P35B (Fragment) 0.6705 0.0492 44121 7.83 3.3 1 33

c85_g1 GRIM-19 0.671 0.029 99274 8.92 2.7 2 45

c39274_g1 0.673 0.00779 46157 4.15 3.1 1 36


38.3 1 28

c13332_g1_orf1 Putative defense protein 3 0.682 0.002 24140 4.77 8.2 1 34

TJ_CDS_07138-1 Fascilin 2-like protein 0.684 0.0141 103058 5.31 6.3 4 133

TJ_CDS_02731-1 AAEL013136-PA 0.6845 0.0111 266997 5.57 1.1 2 54

c5499_g1_orf1 LOC100144940 protein 0.6855 0.0334 67190 5.98 3.6 1 24

c19043_g1_orf1 PIWI 2 (Fragment) 0.6905 0.0298 120993 9.73 6.2 3 48

c2103_g1_orf1 Spatzle protein (Fragment) 0.691 0.00618 39688 5.99 17.6 6 188

TJ_CDS_11198-1 Phosphoglycerate mutase 2 0.7045 0.00754 34349 5.86 9.1 1 145

TJ_CDS_17477-1 Protein VAC14-like protein 0.7065 0.0163 94875 5.69 1.1 1 30

c54243_g1_orf1 TIP41-like protein 0.707 0.0195 38358 5.62 9.6 2 53

c6814_g1_orf1 Putative GTP cyclohydrolase 1 type 2 (Fragment) 0.707 0.0174 49336 6.79 2.1 1 41

c22610_g2_orf1 GH20974 0.7105 0.00989 67398 9.53 1.6 1 30

c3893_g1_orf1 Minichromosome maintenance 3 0.7145 0.0234 100901 5.9 1.2 1 28

TJ_CDS_19237-1 La protein homolog 0.7155 0.0391 66650 8.31 2 1 38

c5004_g1_orf1 Cathepsin B 0.7185 0.017 43526 6.31 11 2 143

TJ_CDS_03208-1 40S ribosomal protein S3a 0.721 0.041 37933 9.72 26.8 5 237

TJ_CDS_01156-1 RNA-binding protein squid 0.724 0.0184 34424 9.17 5.9 1 36

TJ_CDS_24334-1 Aldh9a1a protein 0.7265 0.00815 61167 5.42 3.1 2 30

c19159_g1 0.7275 0.0338 27763 6.22 36.4 5 500

TJ_CDS_23821-1 Methionine aminopeptidase 0.729 0.0164 50575 6.73 7.3 2 58

TJ_CDS_16632-1 Vasa-like (Fragment) 0.737 0.0266 83507 6.33 7.1 2 98

TJ_CDS_05137-1 Ubiquitin carboxyl-terminal hydrolase 0.7415 0.0406 106281 5.24 7.4 3 56

TJ_CDS_23231-2 Proteasome subunit beta type 0.7425 0.00865 34361 8.82 13.1 1 78

c16834_g1_orf1 AGAP003823-PA 0.744 0.0496 100436 5.63 1.4 1 47

TJ_CDS_02394-3 Eukaryotic initiation factor 4A-III 0.745 0.0225 51166 5.61 5.7 2 39

TJ_CDS_22214-1 ATP synthase subunit alpha (Precursor) 0.7465 0.00126 76234 8.96 29.9 14 787

TJ_CDS_05792-1 Inosine-5'-monophosphate dehydrogenase 0.7525 0.0449 68562 6.26 1.4 1 30

TJ_CDS_03626-1 GG13510 0.7535 0.0477 270139 5.69 0.4 1 45

c19770_g1_orf1 Peptidyl-prolyl cis-trans isomerase (Fragment) 0.761 0.0479 19557 8.68 10.3 1 108

c19218_g1_orf1 AAEL014571-PA 0.7615 0.0227 560598 4.92 5.8 16 423

TJ_CDS_01671-1 Down syndrome cell adhesion molecule, putative 0.763 0.0215 184812 6.02 2.8 2 47

TJ_CDS_18054-1 MGC84267 protein 0.765 0.0217 30220 6.16 15.5 3 91

c17709_g1_orf1 Threonyl-tRNA synthetase 0.767 0.03 102638 8.27 2 1 31

TJ_CDS_15386-1 MC family transporter 0.7755 0.0326 34878 9.82 7.6 2 35

c22859_g2_orf1 Putative rna-binding protein sart3 rrm superfamily 0.7755 0.0382 120981 6.13 2.3 2 53

TJ_CDS_26295-1 Putative thioredoxin 2 variant 1 0.78 0.00579 17657 5.12 23.2 3 77

TJ_CDS_07909-1 Chorion peroxidase 0.7825 0.019 91339 6.14 1.9 1 47


c14903_g1_orf1 Mannose-6-phosphate isomerase (Fragment) 0.79 0.0242 53863 5.03 14.3 4 133

c15173_g2_orf1 Fasciclin-like protein 0.7945 0.00774 114513 5.39 8.4 4 132

c61718_g1_orf1 AAEL010969-PA 0.7955 0.0171 50112 7.48 9 3 140

TJ_CDS_10120-1 Kelch domain-containing protein 4 0.7995 0.0365 74142 5.29 4.6 1 89

c18930_g1_orf1 Signal recognition particle subunit SRP68 0.8005 0.0112 85071 7.06 6.7 4 64

TJ_CDS_18130-1 Adenylosuccinate synthetase 0.803 0.0162 55188 6.23 11.7 4 76

c9735_g1_orf1 NADH dehydrogenase flavoprotein 1, mitochondrial 0.8065 0.0443 64419 8.6 18.5 6 275

c2296_g1 0.8095 0.0384 28192 7.79 31.7 4 256

TJ_CDS_00715-1 Cct5-prov protein 0.8155 0.0396 69474 5.84 10 4 94

c9850_g1_orf1 Mcm4 protein 0.819 0.0246 112921 6.31 2.3 2 30

c46982_g1_orf1 E1b-55kD-associated protein, putative 0.8215 0.00178 99313 5.95 9.7 6 232

TJ_CDS_17187-1 Nat10 protein (Fragment) 0.823 0.018 135429 8.55 2.9 2 111

c39207_g1_orf1 Malate dehydrogenase 0.8245 0.0236 42378 6.08 41 13 503

TJ_CDS_05732-1 Sortilin-related receptor 0.827 0.0404 272495 5.11 2.2 4 79

TJ_CDS_05598-5Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform,

putative0.8275 0.0203 82032 5.8 1.8 1 43

c15893_g2_orf1 Stereocilin 0.828 0.0296 155527 5.36 1.4 1 25

c24127_g1_orf1 26S proteasome non-ATPase regulatory subunit 6 0.8325 0.0133 49905 6.82 3.8 1 49



Protein accessionProtein description F2 ratio P value MV [Da] pI AASC [%] MP Score

c61633_g1_orf1 Putative cuticle protein 2.186 0.0466 16684 3.72 3.5 1 34

c56533_g1_orf1 Beta-tubulin (Fragment) 1.946 0.0148 24458 7.05 19.7 5 231

c39450_g1_orf1 Ionotropic GABA-aminobutyric acid receptor BRL3-3b6a 1.772 0.0033 35543 5.06 31.2 5 255

c23401_g2_orf1 AAEL007311-PA 1.77 0.0355 51997 5.3 2.7 2 81

TJ_CDS_15939-1 Troponin C, isoform 1 1.6275 0.0299 22033 3.92 32.6 5 391

c22815_g1_orf1 AGAP004872-PA (Fragment) 1.552 0.0173 51494 5.14 3.4 1 84

c11443_g1_orf1 1.55 0.0231 54789 4.22 5.3 2 35

c39860_g1_orf1 Putative ferric-chelate reductase 1 1.511 0.0485 23864 3.67 7.9 1 314

c25323_g1_orf1 Putative maltase H 1.5035 0.0234 79600 4.5 3.9 1 102

c25288_g1_orf1 Intraflagellar transport protein 122-like protein 1.467 0.00409 22690 5.33 11.2 2 153


c9514_g1 1.45 0.0283 36287 8.68 6 1 75

c39300_g1 1.449 0.00284 21586 4.59 16.3 1 119

c15150_g1_orf1 GI24705 1.4465 0.0306 71632 4.22 7.5 5 90

TJ_CDS_05396-1 Beta tubulin (Fragment) 1.4385 0.0485 35087 6.34 46.8 13 1828

c9652_g1_orf1 Cuticular protein PpolCPR55 1.427 0.0387 34608 8.3 13.5 2 89

TJ_CDS_06418-1 3-carboxymuconate cyclase-like protein 1.4215 0.0219 41443 4.31 15.3 4 70


c13870_g1_orf1 Sulfotransferase family protein 1.402 0.0443 38025 8.85 8.3 2 81

c19159_g1 1.386 0.00165 27763 6.22 36.4 5 500

c2296_g1 1.3815 0.0025 28192 7.79 31.7 4 256

TJ_CDS_19914-3 Flexible cuticle protein 12 1.3795 0.0344 35318 5.29 41.7 10 267

33318289 beta-actin [Tigriopus japonicus] 1.3765 0.0228 46624 5.3 43.2 15 1408

TJ_CDS_18812-1 Apolipoprotein D 1.345 0.00738 23418 3.8 9.6 1 71

TJ_CDS_26956-1 Myosin heavy chain type b 1.3425 0.0492 108245 6.89 36.2 22 1472

c43166_g1 1.341 0.041 33746 5.87 11.5 2 78

c5952_g1 1.3405 0.0383 21140 5.73 13.6 2 42

TJ_CDS_09415-1 Translocon-associated protein subunit gamma 1.34 0.00374 25259 9.61 6 1 52

TJ_CDS_12048-1 V-type proton ATPase subunit H 1.3375 0.0273 63969 6.3 6.2 2 71

TJ_CDS_18680-2 Lactase-phlorizin hydrolase 1.3315 0.0297 63420 4.91 4.5 2 123

c12926_g1_orf1 Past-1 1.331 0.0231 70562 8.33 7.8 4 106

c6598_g1_orf1 Short chain dehydrogenase/reductase family 16C, member 5 1.33 0.00965 44815 8.92 18.4 2 62

c23665_g2_orf1 Myosin heavy chain type b 1.329 0.0367 59122 7.08 32.9 12 515

c18437_g1_orf1 PREDICTED: uromodulin-like 1.3195 0.0249 39097 4.39 17.7 2 123

c54262_g1_orf1 NADH dehydrogenase (Ubiquinone) alpha subcomplex subunit, mitochondrial 1.318 0.018 57032 6.36 15.1 6 137

c3043_g1_orf1 Hypothetical protein LOTGIDRAFT_87751, partial 1.311 0.0286 39915 5.03 10.4 2 90

c13151_g1_orf1 ATP synthase subunit g 1.308 0.0145 15931 9.04 35.7 3 371


TJ_CDS_03879-2 Peptidyl-prolyl cis-trans isomerase 1.304 0.0272 33774 5.09 22.3 3 85

c23556_g1_orf1 Papilin 1.2975 0.0246 190846 5.29 12.5 11 380

c24361_g1_orf1 Alcohol dehydrogenase zinc-binding domain protein 1.2925 0.0163 46714 5.68 9.7 2 95

c41096_g1_orf1 Hypothetical protein NCLIV_049840 1.2925 0.0402 33537 4.17 6.9 2 88

c31594_g1_orf1 RBAM_012210 1.292 0.0348 64393 4.58 20.4 8 166

c17803_g1_orf1 Transmembrane protein, putative 1.287 0.0266 53872 3.68 7.7 3 147

c24443_g1 1.2845 0.019 35787 9.66 17.7 4 142

c31698_g1_orf1 Flavin reductase-like protein 1.274 0.0209 29584 7.88 6.9 2 51

TJ_CDS_25287-1 Putative cuticle protein 1.2685 0.0462 31197 5.62 7.3 2 187

c3203_g1_orf1 Apolipophorin 1.266 0.0239 1028623 5.88 11.8 69 2349

c32316_g1_orf1 RH59876p 1.262 0.034 13749 4.89 31.1 3 184


c21592_g2_orf1 GF14980 1.253 0.0452 201846 6.22 3.2 3 116

c3872_g1_orf1 Innexin 1.2485 0.0166 50026 8.84 4.3 2 38

c19921_g1_orf1 Phosphate carrier protein, mitochondrial 1.243 0.00786 46536 8.97 24 8 325

TJ_CDS_11950-1 Nuclear pore complex protein Nup160-like protein 1.2415 0.0487 179545 5.5 2 2 72

c8443_g1_orf1 Microsomal glutathione S-transferase 1 1.236 0.0404 29796 9.06 15.4 2 102

c46760_g1 1.2355 0.0149 70368 8.89 15 7 374

c21993_g1_orf1 Myosin heavy chain, muscle (Fragment) 1.232 0.0411 169699 5.41 56.8 66 4129

TJ_CDS_04135-1 Copine-8 1.2305 0.0317 74104 5.14 10.9 6 189

c15723_g1_orf1 Galaxin 1.2295 0.0263 61609 3.83 5.9 2 90

c10133_g1_orf1 Nahoda-like protein 1.229 0.0416 56902 5.11 21.3 7 279

c39380_g1_orf1 UDP-glucuronosyltransferase 2A3 (Fragment) 1.223 0.0428 68861 8.09 10.5 5 90

c40743_g1_orf1 Beta-actin, partial 1.21 0.0333 3382 6.75 59.1 1 138

c21719_g1_orf1 Long-chain-fatty-acid--CoA ligase, putative 1.207 0.0215 104888 8.89 5.4 3 44

TJ_CDS_04386-1 Synaptotagmin 1 1.207 0.0491 72717 7.91 14.2 5 118

c21020_g1_orf2 Diet1 1.2035 0.0203 206819 4.5 2.9 3 117



Protein accessionProtein description F2 ratio P value MV [Da] pI

AASC

[%]MP Score

TJ_CDS_13533-1 BCS-1 protein (Fragment) 0.287 0.00893 19662 4.75 27.3 3 296

c18643_g1_orf1 Hypothetical protein 0.3665 0.00352 60254 5.51 6 2 35

TJ_CDS_01131-1 Pro-resilin 0.3795 0.0159 24247 5.51 14 2 89

c12796_g1_orf1 BCS-1 protein (Fragment) 0.382 0.00618 10383 4.73 47 3 173

c8128_g1_orf1 Venom allergen 3 0.3885 0.0431 46503 6.27 22.9 5 186

c1383_g1_orf1 BCS-1 protein (Fragment) 0.39 0.00209 42867 8.66 8.2 2 72

TJ_CDS_22957-1 BCS-1 protein (Fragment) 0.3955 0.0142 21545 5.21 6.1 1 49


c12438_g1_orf1 AAEL007311-PA 0.403 0.0341 49048 5.61 5.5 2 102

TJ_CDS_02233-1 Cuticle protein precursor 0.413 0.0184 15287 4.41 29.8 5 377

c17845_g1_orf1 Putative trypsin-like serine protease (Fragment) 0.4405 0.000569 54133 9.08 22.2 5 448

c10592_g1_orf1 Cuticle protein5a 0.463 0.0426 36177 4.65 7.1 1 44


c18515_g1_orf1 Putative cuticle protein (Fragment) 0.483 0.0123 13661 4.38 32.1 3 73

c15894_g2_orf1 Alpha-carbonic anhydrase 0.498 0.0228 35307 5.02 8.9 2 83

c39337_g1_orf1 Protein msta, isoform B 0.516 0.00658 66506 6.68 2.8 1 84

c62404_g1 0.5235 0.00868 16653 5.79 15.7 2 47

TJ_CDS_24277-1 CREG1 0.5235 0.034 28615 5.53 14.8 2 50

c13332_g1_orf1 Putative defense protein 3 0.53 0.0298 24140 4.77 8.2 1 34

c63275_g1 0.539 0.0248 15244 8.65 35.2 3 112


TJ_CDS_11521-3 Putative SPT transcription factor family member (Fragment) 0.5475 0.0106 73041 8.56 26 9 191

c12546_g2_orf1 Hypothetical protein DAPPUDRAFT_306723 0.5475 0.0302 29443 4.6 4.7 1 41

c20556_g1_orf1 PREDICTED: macrophage mannose receptor 1-like 0.5545 0.0136 35177 8.63 60.8 12 651

TJ_CDS_12619-1 Cuticle protein 19 0.5565 0.0222 22163 6.25 21 2 153

c54160_g1 0.559 0.0317 46098 5.45 12.6 3 97

TJ_CDS_16351-1 Putative carboxypeptidase a 0.5615 0.0181 57939 5.25 5.3 2 83

c5004_g1_orf1 Cathepsin B 0.565 0.0278 43526 6.31 11 2 143

c2707_g1 0.5725 0.035 43923 4.85 9.7 2 61

TJ_CDS_08621-1 Myosin heavy chain, muscle 0.5805 0.0326 126408 5.48 27.3 20 688

c2291_g1_orf1 40S ribosomal protein S13 0.5855 0.0131 24660 10.53 25.6 3 110

c5198_g1_orf1 Putative ascorbate peroxidase 0.5855 0.0468 48617 5.88 11.6 4 173

TJ_CDS_15715-1 Cathepsin i (Fragment) 0.5865 0.01 43845 5.69 3.6 1 69

c61551_g1_orf1 CDGSH iron sulfur domain-containing protein 2 homolog 0.5945 0.00549 20345 8.61 8.3 1 269

c14374_g2_orf1 GM12828 0.602 0.0479 90015 4.6 7 4 206

c17645_g1 0.6045 0.0137 16763 5.51 33.9 3 77

c13167_g1_orf1 40S ribosomal protein S7 0.619 0.0434 30859 10.14 25.8 5 156



c20184_g1_orf1 Xanthine dehydrogenase/oxidase 0.625 0.0119 138387 5.36 3.4 3 36

c21499_g1_orf1 Lysosomal aspartic protease 0.625 0.0119 48256 8.73 33.9 9 559

c15173_g2_orf1 Fasciclin-like protein 0.6275 0.0179 114513 5.39 8.4 4 132


c34440_g1 0.637 0.0333 18991 4.94 21.5 3 96

c4699_g1_orf1 Metallopeptidase M72 family protein 0.6525 0.0174 63800 5.47 13.5 7 107

c12123_g2 0.6545 0.0138 28717 4.49 10 2 71

TJ_CDS_10340-1 60S ribosomal protein L31 0.657 0.0426 16966 10.15 45.9 4 70

c54217_g1 0.664 0.0435 48027 8.54 5.8 2 211

TJ_CDS_21737-2 Putative SPT transcription factor family member (Fragment) 0.664 0.0492 79984 5.9 12.9 8 222

TJ_CDS_03540-1 Lamin-C 0.6665 0.041 94494 6.06 19.9 10 234

TJ_CDS_05958-1 60S ribosomal protein L23 0.6685 0.0393 18149 10.69 20 2 98


TJ_CDS_24893-1 BCS-1 0.6775 0.0424 34112 5.35 11.2 2 347


c31699_g1 0.6815 0.00899 48749 6.09 10.3 4 53

c3857_g1_orf1 Diaminopimelate decarboxylase 0.6815 0.023 58727 5.87 4 1 42

c39563_g1 0.6895 0.0256 19603 8.3 16.1 2 45

c5761_g1_orf1 Cellobiohydrolase CHBI 0.6925 0.00518 59436 4.4 37.4 14 1627

TJ_CDS_01091-1 40S ribosomal protein S26 0.6925 0.0135 18037 10.87 23.2 2 94

TJ_CDS_07457-1 Putative dna replication licensing factor mcm4 component (Fragment) 0.694 0.0208 106483 5.24 2.5 2 35

c39470_g1 0.698 0.019 42546 5.26 21.7 6 197

TJ_CDS_06616-2 Col25a1 protein 0.698 0.0358 40504 5.62 8.2 2 113

c54197_g1_orf1 AGAP011936-PA 0.7105 0.0341 70809 4.93 8.8 4 275

c7794_g1_orf1 Putative serine carboxypeptidase CPVL 0.711 0.0154 57755 4.63 1.7 1 39

c48702_g1_orf1 Peptide methionine sulfoxide reductase MsrB 0.711 0.0374 29600 9.19 8.7 1 33


c48014_g1_orf1 Hemicentin-1 0.715 0.0312 49703 5.17 5.5 2 74

TJ_CDS_13301-1 AGAP003895-PA 0.7155 0.0235 36586 4.29 7 2 101

c14530_g1 0.717 0.0247 58660 8.42 9.3 4 113

TJ_CDS_23796-1 Sarcoplasmic calcium-binding protein, beta chain 0.7205 0.0262 26726 4.83 46.6 7 284

339787884"glutathione S-transferase 1-like protein, partial [Tigriopus

californicus]"0.724 0.0277 17586 4.73 21.4 2 65

c20764_g1_orf1 N-formylglutamate amidohydrolase 0.7255 0.0104 35465 5.34 6 1 70

c31495_g1_orf1 GH23633 0.7255 0.0243 25371 6.13 17.7 2 108

c61718_g1_orf1 AAEL010969-PA 0.7305 0.0483 50112 7.48 9 3 140

c2103_g1_orf1 Spatzle protein (Fragment) 0.733 0.031 39688 5.99 17.6 6 188


c5260_g1_orf1 Polyadenylate-binding protein 1 0.738 0.0316 85965 9.16 6.5 4 96

TJ_CDS_02511-1 Ubiquitin carboxyl-terminal hydrolase isozyme L3 0.7405 0.0404 29110 4.52 21.3 4 98

c19680_g1_orf1 Tropomyosin-1, isoforms 9A/A/B 0.7405 0.0257 40452 4.76 18.8 4 162

TJ_CDS_22533-1 Transmembrane 9 superfamily member 3 0.742 0.0074 74680 5.94 2.1 1 75

TJ_CDS_16184-1 Adapter molecule Crk 0.743 0.00495 35461 6.08 9.1 2 67

TJ_CDS_07816-2 Synaptosomal-associated protein 0.7435 0.031 26490 4.52 20.6 3 81

c13053_g1_orf1 Myosin-9, putative 0.7445 0.0261 173429 6.11 15.3 15 359

TJ_CDS_16540-1 AAEL013766-PA 0.7455 0.0163 53154 4.7 8.6 3 54

c31972_g1_orf1 Beta-hexosaminidase 0.747 0.0101 92827 6.11 2.1 1 33

c20430_g1_orf1 Vitellogenin 2 0.7475 0.0265 238394 8.4 63.8 93 11420

TJ_CDS_00237-1 Putative methylthioribulose-1-phosphate dehydratase 0.7495 0.0267 59122 9.82 2.7 1 36

c31810_g1_orf1 Di-N-acetylchitobiase 0.7515 0.0346 43274 4.35 5.4 2 55

c54389_g1_orf1 Mesoderm development candidate 2 0.7535 0.0245 40145 6.56 10.5 2 25

c20729_g1_orf1 PREDICTED: maltase-glucoamylase, intestinal-like 0.7595 0.0146 107031 4.62 8.1 5 218

c39208_g1_orf1 Lysozyme 0.7595 0.0198 19382 5.75 43.3 5 410

c9057_g1 0.76 0.0186 31921 9.66 6.7 1 101

TJ_CDS_16409-2 Tudor staphylococcal nuclease 0.766 0.0109 118256 6.5 9.5 6 275

TJ_CDS_00439-1 Ras-related protein ORAB-1 0.768 0.0192 26464 5.28 28.4 4 164

c20843_g1_orf1 Dumpy, isoform J 0.7715 0.00975 98387 6.3 10.9 5 191

TJ_CDS_03342-1 Ribosomal protein S27-3 0.7715 0.0153 24676 9.73 53.2 8 205

c23872_g1 0.7735 0.0491 41020 6.52 18.9 5 155

c21276_g1_orf1 Homologue of Sarcophaga 26,29kDa proteinase 0.7745 0.0127 74089 5.7 9.2 4 141

c23999_g1 0.775 0.0311 29166 4.07 25.6 4 508

TJ_CDS_15107-2 Myosin tail 0.7775 0.0186 272312 5.97 9.8 13 443

c21806_g2_orf1 Vitellogenin 0.7775 0.0357 237746 8.74 63.2 95 9350

c61871_g1_orf1 Omega class glutathione S-transferase 0.7815 0.0131 36736 6.52 19.9 4 82

c39194_g1_orf1 Cathepsin L 0.7855 0.0371 56245 4.65 9.8 2 81

c6356_g1_orf1 Agrin 0.7875 0.0493 230389 4.37 2.6 3 71

TJ_CDS_16479-1 Heat shock protein 40 (Fragment) 0.7935 0.017 53673 5.89 5.8 2 102

c1312_g1_orf1 Succinyl-CoA:3-ketoacid-coenzyme A transferase 0.806 0.00656 65357 6.32 8 3 207

c16486_g1 0.808 0.0298 42692 5.58 13.7 5 121

c39130_g1_orf1 AAEL017263-PA 0.8105 0.0486 143055 4.97 6.1 4 105

c16112_g1_orf1 Rho-associated protein kinase 2 0.813 0.0272 196887 6.05 2 1 107

c17495_g1_orf1 T-complex protein 1 subunit alpha 0.814 0.0205 76878 6.12 6.4 4 109

c13226_g1 0.8145 0.012 20340 8.2 39.3 7 537


TJ_CDS_04697-1 Propionyl-CoA carboxylase beta chain, mitochondrial 0.816 0.0104 69459 7.99 9.1 4 122

c1975_g1_orf1 Venom allergen 3 0.822 0.0357 46458 6.12 6.4 2 181

c23296_g1_orf1 Chromatin-remodeling complex ATPase chain Iswi 0.8285 0.0278 143767 8.87 2.9 3 95

TJ_CDS_06025-1 40S ribosomal protein S6 0.831 0.0188 35189 10.88 20.9 2 138




Protein accessionProtein description F5 ratio P value MV [Da] pI AASC [%] MP Score

TJ_CDS_02480-1 1.8405 0.00265 22273 5.87 24.6 3 246

c54324_g1 1.6365 0.0165 6993 7.55 24.5 2 102

c11477_g1_orf1 Histone H2A 1.5825 0.018 22515 10.9 9.7 2 69

c1381_g1 1.5665 0.0197 53815 4.3 12 5 159

TJ_CDS_01131-1 Pro-resilin 1.5235 0.0164 24247 5.51 14 2 89

c42612_g1_orf1 Tropomyosin 1.5225 0.0468 22266 4.79 13.3 2 51

c46913_g1 1.477 0.0147 23712 4.67 25.3 3 180

TJ_CDS_14513-1 Myosin light chain alkali 1.417 0.0427 23270 4.83 42.6 7 163

c17282_g1_orf1 Calmin-like protein (Fragment) 1.4165 0.0145 89364 5.16 32 15 340

c23637_g1_orf1 Myosin heavy chain, nonmuscle or smooth muscle 1.4085 0.0459 216437 5.84 44.4 65 3411

c25418_g1 1.408 0.025 35535 5.02 6.3 1 277

c23554_g1_orf1 Poly [ADP-ribose] polymerase 1 1.3955 0.0105 314446 8.35 1.4 2 49

c48014_g1_orf1 Hemicentin-1 1.3925 0.0445 49703 5.17 5.5 2 74

c46777_g1_orf1 Metal ion binding protein 1.3865 0.0272 75741 6.59 24.5 12 356

c46813_g1_orf1 Extended synaptotagmin 1.381 0.0417 107505 5.51 2.9 2 119

c61720_g1_orf1 PREDICTED: protein lethal(2)essential for life-like 1.3775 0.0463 227675 5.39 20.3 26 771

TJ_CDS_00649-2 Myosin-7 1.372 0.0325 173741 5.52 23.3 25 575

TJ_CDS_00648-1 Myosin heavy chain 1.3545 0.0475 52135 5.07 15 4 132

TJ_CDS_22797-1 Putative 4a-hydroxytetrahydrobiopterin dehydratase 1.3405 0.0364 17153 9.03 9.9 1 64

TJ_CDS_16787-1 Myosin-9, putative 1.3165 0.0111 265385 5.7 41.3 75 4795

TJ_CDS_26956-1 Myosin heavy chain type b 1.3075 0.0114 108245 6.89 36.2 22 1472

c23520_g1_orf1 Polysaccharide deacetylase (Precursor) 1.298 0.032 38873 9.53 8 2 125

c5769_g1_orf1 Heat shock protein beta-1 1.2825 0.00113 31305 7.82 5.8 1 30

c13444_g1_orf1 Cullin-associated NEDD8-dissociated protein 1 1.278 0.0412 163830 5.96 3.8 3 97

c61728_g1_orf1 5-oxoprolinase 1.271 0.0117 160159 5.92 4.3 2 42


TJ_CDS_07722-3 Elongation factor Tu 1.259 0.0295 73989 5.2 3.5 2 62

TJ_CDS_22949-3 Proteasome subunit beta type 1.258 0.0394 26417 6.31 5.4 1 45

c54460_g1_orf1 D-lactate dehydrogenase 1.257 0.047 53994 7.62 21.9 7 155

c4171_g1_orf1 Glucosamine-6-phosphate isomerase 1.2565 0.0335 42779 6.95 8.6 2 51

c39207_g1_orf1 Malate dehydrogenase 1.2555 0.0386 42378 6.08 41 13 503

TJ_CDS_15939-1 Troponin C, isoform 1 1.252 0.0202 22033 3.92 32.6 5 391

TJ_CDS_03008-1 Glucose-6-phosphate isomerase 1.238 0.016 72757 5.97 18.8 6 250

TJ_CDS_11765-1 Eukaryotic translation initiation factor 3 subunit M 1.236 0.0431 52143 5.98 5.9 2 51

TJ_CDS_00410-1 Glyceraldehyde 3-phosphate dehydrogenase 1.2215 0.0129 40824 6.66 40.8 9 1144

c12037_g1_orf126S proteasome regulatory complex subunit

RPN2/PSMD11.221 0.0431 129179 5.3 5.2 5 94

TJ_CDS_08623-1 Myosin heavy chain isoform 1 1.22 0.0491 163054 5.61 52.4 53 3747

c32080_g1_orf1 Carboxylesterase 1.2135 0.0432 93570 5.6 4.1 2 101

TJ_CDS_14266-1 Hexokinase 1.208 0.0306 77158 6.2 26.1 8 262

c12783_g1_orf1 Fructose-bisphosphate aldolase 1.203 0.00941 49332 6.78 15.2 5 157



Protein accession Protein description F5 ratio P value MV [Da] pIAASC

[%]MP Score

c20088_g1_orf1 AAEL007311-PA 0.4655 0.000596 51558 5.48 4.8 2 60

c17879_g1_orf1 AAEL010188-PA 0.48 0.00612 41291 4.89 6.8 2 35

c12828_g1_orf1 Nuclear pore membrane glycoprotein 210 0.4955 0.0296 118900 4.78 6 4 154

c62404_g1 0.506 0.036 16653 5.79 15.7 2 47





c11005_g1_orf1 PREDICTED: survival motor neuron protein 1-like isoform X1 0.5645 0.027 102054 5.03 1.2 1 44

c15358_g1 0.58 0.0484 32111 4.81 21.5 4 595

c21708_g2_orf1 Intraflagellar transport protein 122-like protein 0.5835 0.0298 46098 6.13 3.2 1 67


c3212_g1_orf1 Serine protease nudel 0.607 0.0178 227382 5.06 9.3 12 193


c35874_g1 0.6235 0.0346 61767 4.44 2.6 1 37

c14564_g1_orf2 Cyanophycinase 0.634 0.0157 24114 4.06 16.4 2 82

c18209_g1_orf1 Peroxidase 0.6395 0.0274 85506 6.01 12.7 7 176

c20164_g1_orf1 Acid trehalase-like protein 1 0.6415 0.0346 87709 4.74 4.1 3 79

c16808_g1_orf1 Stress protein DDR48 0.6435 0.0259 130746 4.78 5.1 5 60

c15469_g1 0.6615 0.0254 68885 9.66 2.1 1 48

c15150_g1_orf1 GI24705 0.6615 0.0047 71632 4.22 7.5 5 90

c143_g1_orf1 PREDICTED: hepatic lectin-like 0.666 0.0305 21435 4.44 21.2 3 125

c3180_g1 0.677 0.0335 72929 5.62 17.3 9 513

c14374_g2_orf1 GM12828 0.679 0.0178 90015 4.6 7 4 206

c5493_g1_orf1 Cuticle protein 0.6825 0.0291 37338 6.33 6.7 2 68

c47303_g1 0.685 0.0121 29230 5.2 19.1 3 135

TJ_CDS_15264-1 Long-chain-fatty-acid--CoA ligase 3 0.6855 0.0334 94745 7.87 3.8 2 71

c6356_g1_orf1 Agrin 0.687 0.0406 230389 4.37 2.6 3 71

c20764_g1_orf1 N-formylglutamate amidohydrolase 0.691 0.00824 35465 5.34 6 1 70

c61590_g1_orf1 Ferritin 0.6925 0.0197 26030 5.41 59.6 11 1051

c31810_g1_orf1 Di-N-acetylchitobiase 0.6985 0.0285 43274 4.35 5.4 2 55

c2755_g1_orf1 FK506-binding protein 7 0.7005 0.0266 29895 4.6 12.2 3 363

c46775_g1_orf1 AAEL007657-PA 0.701 0.0404 121252 4.93 10.2 7 475

c13296_g1 0.703 0.0471 22000 4.9 27.6 3 244

c31552_g1_orf1 Heme-binding protein 2 0.7045 0.0462 26703 4.56 4.2 1 76

c21018_g1_orf1 GM11273 0.7055 0.0464 109549 4.78 8.4 6 172

c13467_g1_orf1 Thymidine kinase 2, mitochondrial 0.7065 0.0358 43785 9.21 10.5 2 27

c8328_g1_orf1Putative this conserved domain family includes a large number

(Fragment)0.708 0.0457 71495 4.46 3.2 2 34

c14907_g1_orf1 Cytochrome c 0.711 0.00881 19398 9.56 36.2 4 116

c15723_g1_orf1 Galaxin 0.7155 0.048 61609 3.83 5.9 2 90

c18974_g1_orf1 Protein jagged-1 0.7175 0.00563 43969 5.05 16 5 126


c39860_g1_orf1 Putative ferric-chelate reductase 1 0.7215 0.00343 23864 3.67 7.9 1 314

c31594_g1_orf1 RBAM_012210 0.7235 0.015 64393 4.58 20.4 8 166

TJ_CDS_13424-1 Carboxypeptidase B 0.7245 0.0289 52426 4.72 10.6 2 108

TJ_CDS_07157-1 Putative maternal expression at 31b 0.727 0.0187 58317 8.02 24.1 8 236

c56245_g1_orf1 PREDICTED: d-dopachrome decarboxylase 0.7275 0.0432 18359 8.46 9 1 63



c11144_g1_orf1 Putative phospholipase B-like 2 0.736 0.0481 75151 8.79 5.3 1 53

c5761_g1_orf1 Cellobiohydrolase CHBI 0.7375 0.0158 59436 4.4 37.4 14 1627

c19132_g1 0.744 0.0422 63926 4.86 7.6 3 154

c13683_g1_orf1 Protein disulfide isomerase, putative 0.747 0.0402 71265 5.01 22.4 9 292

c13578_g1_orf2 Putative villin-1 0.7525 0.00386 110019 5.29 9.4 6 241

c5690_g1_orf1 GA21047, isoform A 0.755 0.0467 35685 5.59 9.4 2 85

c20709_g1_orf1 Mannose C-type lectin receptor 0.763 0.0242 121929 4.41 3.2 2 87

c5733_g1_orf1 Lipase maturation factor 2 0.7735 0.0239 86616 9.16 2.9 2 50

c39653_g1_orf1 Aminopeptidase 0.774 0.0253 86846 5.07 5.7 3 54

TJ_CDS_17075-2 Cytokine receptor 0.7825 0.00439 155323 5.62 1 1 77

c46825_g1_orf1 Chymotrypsin-like proteinase 0.7825 0.0424 33855 5.39 23.3 4 1118

TJ_CDS_12945-1 Fatty acid-binding protein 2 0.7835 0.0162 23167 6 19.2 3 159

c23328_g2_orf1 Putative low-density lipoprotein receptor 0.7845 0.0486 429084 4.87 8.3 18 710

c61748_g1_orf1 C-type lectin-galactose binding 0.785 0.0148 58743 6.09 14.8 4 162

TJ_CDS_01675-1 Down syndrome cell adhesion molecule isoform (Fragment) 0.7855 0.0371 177972 6.18 2.9 3 79

c8758_g1_orf1 GE14248 0.7855 0.0252 69937 4.55 10.2 4 171

c21398_g1_orf1 Collagenase 0.7875 0.0225 36568 5.97 18.4 3 476

TJ_CDS_00392-1 GD14343 0.788 0.027 39515 3.7 4.7 1 129

TJ_CDS_18188-1 60S ribosomal protein L10 0.791 0.00914 30466 10 21.5 5 58

c54140_g1_orf1 Protein disulfide isomerase prolyl 4-hydroxylase beta subunit 0.7935 0.0323 71734 4.77 48.9 25 2386

c54197_g1_orf1 AGAP011936-PA 0.795 0.00932 70809 4.93 8.8 4 275

TJ_CDS_07829-1 Serine proteinase-like 2a 0.7955 0.0233 71901 4.43 28.1 8 1347

c16336_g1_orf1 Transmembrane emp24 domain-containing protein 2 0.7985 0.0457 29433 5.54 15.4 3 92

TJ_CDS_17501-1 Dehydrogenase/reductase SDR family member 1 0.8025 0.037 38054 7.66 7.8 2 61

c27773_g1 0.804 0.0162 5801 6.71 12.8 2 57

c21973_g1_orf1 Opsin (Fragment) 0.805 0.0261 50046 7.36 3.2 1 117

TJ_CDS_21526-1 Chromosome 11 SCAF14979, whole genome shotgun sequence 0.8065 0.0443 26028 9.54 4.9 1 71

c46862_g1_orf1 Trypsin type 4 (Fragment) 0.808 0.0463 30571 4.41 19.5 5 397

c5952_g1 0.809 0.0233 21140 5.73 13.6 2 42

TJ_CDS_13506-2 DnaJ-like protein subfamily C member 10 0.8135 0.0222 104449 5.76 5.7 4 90

TJ_CDS_26035-1 AGAP000851-PA 0.8135 0.0222 14024 9.15 11.5 1 86

c8241_g1_orf1 Alpha-glucosidase 0.82 0.0318 75829 4.5 19.3 8 427

c61657_g1 0.82 0.0424 44420 9.12 7.5 2 130

TJ_CDS_01908-1 AAEL005376-PA 0.822 0.0143 189323 6.32 1.3 2 79

c23328_g1_orf1 AAEL009869-PA 0.823 0.018 179795 4.82 12.4 10 515


c16705_g1_orf2 Cytochrome P450 2L1 0.8265 0.0385 64503 8.86 7.2 3 86

c19218_g1_orf1 AAEL014571-PA 0.8275 0.0203 560598 4.92 5.8 16 423

c22233_g2_orf1 Protein jagged-1 0.8305 0.0244 48854 4.52 18.7 6 306


c31682_g1_orf1 Cytochrome b-c1 complex subunit 7 0.8315 0.00944 23546 8.97 24.2 5 376


Table S8 Distribution of enriched protein pathways for the differentially expressed proteins in F0.

Category Term description Accession number Count Fisher's exact test P value Benjamini-Hochberg's corrected P value

Up-regulated

Cellular component Intrinsic component of membrane GO:0031224 10 0.0008 0.0021

Cellular component Integral component of membrane GO:0016021 10 0.0008 0.0021

Cellular component Membrane GO:0016020 15 0.0014 0.0023

Cellular component Membrane part GO:0044425 10 0.0074 0.0093

Down-regulated

Cellular component Ribonucleoprotein complex GO:0030529 12 0.0000 0.0002

Cellular component Ribosome GO:0005840 11 0.0000 0.0002

Cellular component MCM complex GO:0042555 3 0.0006 0.0052

Cellular Component Macromolecular complex GO:0032991 20 0.0007 0.0052

Cellular component Intracellular organelle GO:0043229 20 0.0014 0.0060

Cellular component Organelle GO:0043226 20 0.0014 0.0060

Cellular component Intracellular part GO:0044424 25 0.0014 0.0060

Cellular component Nucleus GO:0005634 8 0.0027 0.0098

Cellular component Non-membrane-bounded organelle GO:0043228 13 0.0052 0.0150

Cellular component Intracellular non-membrane-bounded organelle GO:0043232 13 0.0052 0.0150

Molecular function Nucleic acid binding GO:0003676 24 0.0000 0.0000

Molecular function Structural constituent of ribosome GO:0003735 11 0.0000 0.0007

Molecular function DNA binding GO:0003677 10 0.0000 0.0007

Molecular function Organic cyclic compound binding GO:0097159 37 0.0006 0.0122

Molecular function Heterocyclic compound binding GO:1901363 37 0.0006 0.0122

Molecular function Lipid transporter activity GO:0005319 3 0.0014 0.0223

Molecular function DNA helicase activity GO:0003678 3 0.0020 0.0268

Biological process Cellular biosynthetic process GO:0044249 26 0.0000 0.0000

Biological process Organic substance biosynthetic process GO:1901576 26 0.0000 0.0000

Biological process Cellular macromolecule metabolic process GO:0044260 31 0.0000 0.0001

Biological process Biosynthetic process GO:0009058 26 0.0000 0.0001

Biological process Cellular macromolecule biosynthetic process GO:0034645 19 0.0000 0.0001

Biological process Macromolecule biosynthetic process GO:0009059 19 0.0000 0.0001

Biological process Gene expression GO:0010467 18 0.0000 0.0002

Biological process Cellular metabolic process GO:0044237 40 0.0000 0.0004

Biological process DNA replication GO:0006260 6 0.0000 0.0008

Biological processNucleobase-containing compound metabolic

processGO:0006139 17 0.0001 0.0010

Biological process Macromolecule metabolic process GO:0043170 37 0.0001 0.0010

Biological process Heterocycle metabolic process GO:0046483 18 0.0001 0.0011

Biological process Cellular aromatic compound metabolic process GO:0006725 18 0.0001 0.0011

Biological process Nucleic acid metabolic process GO:0090304 12 0.0001 0.0011

Biological process DNA metabolic process GO:0006259 6 0.0001 0.0012

Biological process Organic cyclic compound metabolic process GO:1901360 18 0.0001 0.0012

Biological process Cellular nitrogen compound metabolic process GO:0034641 18 0.0002 0.0016

Biological process Translation GO:0006412 13 0.0003 0.0029

Biological process Organophosphate biosynthetic process GO:0090407 7 0.0009 0.0077

Biological process DNA-dependent DNA replication GO:0006261 3 0.0010 0.0077

Biological process DNA replication initiation GO:0006270 3 0.0010 0.0077

Biological process Nucleotide-sugar biosynthetic process GO:0009226 2 0.0010 0.0077

Biological process Lipid transport GO:0006869 3 0.0022 0.0158

Biological process Lipid localization GO:0010876 3 0.0022 0.0158

Biological process Organic hydroxy compound biosynthetic process GO:1901617 2 0.0029 0.0190

Biological process Nucleotide-sugar metabolic process GO:0009225 2 0.0029 0.0190

Biological process Cellular protein metabolic process GO:0044267 26 0.0053 0.0333

Biological process Aromatic compound biosynthetic process GO:0019438 7 0.0072 0.0440

KEGG pathway DNA replication ko03030 6 0.0000 0.0006

KEGG pathway Ribosome ko03010 11 0.0002 0.0042

KEGG pathway Cell cycle ko04110 5 0.0007 0.0110

KEGG pathway Meiosis - yeast ko04113 4 0.0022 0.0275

Protein domain Vitellinogen, open beta-sheet, subdomain 1 IPR015817 3 0.0001 0.0121

Protein domain Vitellinogen, open beta-sheet IPR015255 3 0.0002 0.0148

Protein domain Lipid transport protein, beta-sheet shell IPR015819 3 0.0006 0.0196

Protein domain Rubredoxin-type fold IPR004039 2 0.0007 0.0196

Protein domain Lipid transport protein, N-terminal IPR001747 3 0.0009 0.0196

Protein domain Vitellinogen, beta-sheet N-terminal IPR015816 3 0.0009 0.0196

Protein domain Vitellinogen, superhelical IPR011030 3 0.0009 0.0196

Protein domainMini-chromosome maintenance, DNA-dependent

ATPaseIPR001208 3 0.0009 0.0196

Protein domain Nucleic acid-binding, OB-fold IPR012340 5 0.0011 0.0215

Protein domain von Willebrand factor, type D domain IPR001846 3 0.0025 0.0436

Only the terms with a corrected P value < 0.05 were considered to be enriched.



Up-regulated

Cellular component Organelle part GO:0044422 9 0.0017 0.0462

Cellular component Intracellular organelle part GO:0044446 9 0.0017 0.0462

Down-regulated

Cellular component Ribosome GO:0005840 8 0.0012 0.0135

Cellular component Ribonucleoprotein complex GO:0030529 8 0.0025 0.0135



Molecular function Structural molecule activity GO:0005198 19 0.0000 0.0000

Molecular function Structural constituent of cuticle GO:0042302 10 0.0000 0.0000

Molecular function Cysteine-type peptidase activity GO:0008234 6 0.0001 0.0018

Molecular function Structural constituent of ribosome GO:0003735 8 0.0008 0.0115

Biological process Macromolecule metabolic process GO:0043170 27 0.0001 0.0080

Biological process Protein metabolic process GO:0019538 23 0.0003 0.0097

KEGG pathway Ribosome ko03010 8 0.0001 0.0081

Protein domain Peptidase C1A, papain C-terminal IPR000668 5 0.0001 0.0072

Protein domain Proteinase inhibitor I29, cathepsin propeptide IPR013201 3 0.0007 0.0435




Up-regulated

Cellular component Myosin complex GO:0016459 6 0.0000 0.0000

Cellular component Actin cytoskeleton GO:0015629 6 0.0000 0.0000

Cellular component Protein complex GO:0043234 9 0.0000 0.0000

Cellular component Cytoskeletal part GO:0044430 6 0.0000 0.0003

Cellular component Cytoskeleton GO:0005856 6 0.0001 0.0003

Cellular component Macromolecular complex GO:0032991 9 0.0001 0.0003

Cellular component Organelle part GO:0044422 7 0.0002 0.0007

Cellular component Intracellular organelle part GO:0044446 7 0.0002 0.0007



Cellular component Intracellular part GO:0044424 9 0.0035 0.0084

Cellular component Intracellular GO:0005622 9 0.0097 0.0211

Cellular component Cell GO:0005623 9 0.0149 0.0242

Cellular component Cell part GO:0044464 9 0.0149 0.0242

Cellular component Intracellular organelle GO:0043229 7 0.0149 0.0242

Cellular component Organelle GO:0043226 7 0.0149 0.0242

Molecular function Motor activity GO:0003774 6 0.0000 0.0006

Molecular functionIntramolecular oxidoreductase activity,

Interconverting aldoses and ketosesGO:0016861 2 0.0012 0.0181

Molecular function Nucleoside-triphosphatase activity GO:0017111 7 0.0014 0.0181

Molecular function Intramolecular oxidoreductase activity GO:0016860 2 0.0016 0.0181

Molecular function Pyrophosphatase activity GO:0016462 7 0.0017 0.0181

Molecular functionHydrolase activity, acting on acid Anhydrides, in

phosphorus-containing anhydridesGO:0016818 7 0.0019 0.0181

Molecular function Hydrolase activity, acting on acid anhydrides GO:0016817 7 0.0022 0.0181

Biological process Carbohydrate metabolic process GO:0005975 8 0.0000 0.0001

Biological process Single-organism carbohydrate metabolic process GO:0044723 4 0.0005 0.0160

Biological process Glucose metabolic process GO:0006006 2 0.0006 0.0160

KEGG pathway Glycolysis / Gluconeogenesis ko00010 4 0.0003 0.0061

KEGG pathway Microbial metabolism in diverse environments ko01120 6 0.0005 0.0061

KEGG pathway Carbon fixation in photosynthetic organisms ko00710 3 0.0005 0.0061

KEGG pathway Carbon metabolism ko01200 5 0.0008 0.0072

KEGG pathway Amino sugar and nucleotide sugar metabolism ko00520 3 0.0020 0.0146

KEGG pathway Biosynthesis of antibiotics ko01130 5 0.0048 0.0289

KEGG pathway Fructose and mannose metabolism ko00051 2 0.0096 0.0496

Protein domain Myosin tail IPR002928 5 0.0000 0.0003

Protein domain Myosin head, motor domain IPR001609 5 0.0000 0.0008

Protein domain Myosin-like IQ motif-containing domain IPR027401 4 0.0001 0.0009

Protein domain Alpha crystallin/Hsp20 domain IPR002068 2 0.0037 0.0469

Down-regulated

Cellular component Cytochrome complex GO:0070069 2 0.0007 0.0148

Cellular component Respiratory chain GO:0070469 2 0.0015 0.0148

Cellular component Mitochondrial respiratory chain GO:0005746 2 0.0015 0.0148

Molecular function Serine-type endopeptidase activity GO:0004252 6 0.0007 0.0330

Molecular function Serine-type peptidase activity GO:0008236 6 0.0023 0.0334

Molecular function Serine hydrolase activity GO:0017171 6 0.0023 0.0334

Protein domain Epidermal growth factor-like domain IPR000742 8 0.0000 0.0014

Protein domain Six-bladed beta-propeller, TolB-like IPR011042 4 0.0003 0.0118

Protein domain Peptidase S1 IPR001254 6 0.0005 0.0118

Protein domain Trypsin-like cysteine/serine peptidase domain IPR009003 6 0.0006 0.0118

Protein domain DOMON domain IPR005018 3 0.0010 0.0174


Documents

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