Application Notes Aracus - Gulf Bio Analytical · Application Notes Aracus membraPure GmbH Wolfgang Küntscher Str. 14 ... 24 55.2 Nle 4 42620 Urea 25 56.9 Tyr 5 18.0 Asp 26 60.2

Application Notes

Aracus

membraPure GmbH

Wolfgang Küntscher Str. 14

16761 Hennigsdorf / Berlin

Mail: [email protected]

Tel.: 03302 / 201 20 0

Fax: 03302 / 201 20 21

___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

membraPure GmbH · Wolfgang-Küntscher-Straße 14 · D-16761 Hennigsdorf

Tel.: +49 (0)3302 201 20-0 · Email: [email protected]

Index

• Physiological Extended 54 amino acid

Clinical Applications

• Physiological (125x3/iVD) 42 amino acid

• Physiological (150x4)

• Diagnostic short (MSUD)

Food and Feedstuff Applications

• Hydrolysate (125x3)

• Hydrolysate (125x4)

• Hydrolysate PLUS (TRP)

• Feedstuff

• 24 Amino Acids Feedstuff-short

Biogene amine and others

• Biogenic Amine

• Glyphosate and AMPA

• Stress factors plants

Definitions of abbreviations

1 – 2

5 – 6

7 – 8

9 – 10

13 – 14

15 – 16

17 – 18

19 – 20

21 – 22

25 – 26

27 – 28

29 – 30

31 – 32

Seite 1 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



A P P L I C A T I O N N O T E

Physiological Extended

Equipment .................... : ARACUS

Column ......................... : Lithium system, 150 mm BL x 4.0 mm ID

Ammonium Absorber, 40 mm x 2.0 mm ID

Eluents .......................... : Lithium system - Eluent A to Eluent F

Runtime ........................ : 128 min

Gradient ........................ : Program in Steps

Eluent Flow ................... : 240 µl/min

Column Temperature ... : 35 °C to 74 °C

Injection Volume .......... : 20 µl

Reagent ........................ : Ninhydrin (Post-Column Derivatization)

Reagent Flow ............... : 120 µl/min

Reactor Temperature ... : 125 °C

Detection ...................... : Visible – 570 nm (Channel A) / 440 nm (Channel B)

Sample .......................... : Physiological standard Solution

Standard Solution with 100 nmol/ml (Channel A / 570nm)

[min.]Time

0 20 40 60 80 100 120 140

[mV]

Voltage

0

100

200

300

400

500

3,7

3,8

SULFO

6,0

TAU

7,1

PEA

9,6

Ure

a

13,7

GLUA

21,3

ASP

24,5

HYP

28,2

THR

30,3

SER

34,2

ASN

36,5

GLU

39,3

GLN

42,5

SAR

45,6

AAA

48,9

50,8

GLY

53,7

ALA

56,3

CIT

59,5

ABU

65,9

VAL

67,6

SAC

68,0

CYS2

68,6

ASA

69,2

PIP

69,7

CYSTA

70,2

HCI

71,2

MET

71,9

AIL

E

73,5

ILE

75,4

LEU

77,5

NO

RLEU

80,0

TYR

86,3

PHE

91,0

HCY2

95,5

BALA

96,8

ANHYD A

SA

97,2

BAIB

99,5

ALEVA

100,1

GABA

101,2

KYN

102,7

HIS

104,0

1M

HIS

106,2

3M

HIS

108,9

TRP

112,7

CAR

113,7

HCAR 117,1

ANS

119,6

AEC

120,3

HYL

121,8

ORN

123,6

LYS

126,6

Am

monia

128,9

ETN

135,1

ARG

[min.]Time

65 70 75 80

[mV]

Voltage

0

50

100

150

200

65,9

0 V

AL

67,5

8 S

AC

67,9

7 C

YS2

68,5

8 A

SA

69,1

7 P

IP

69,7

0 C

YSTA

70,2

3 H

CI

71,2

1 M

ET

71,9

1 A

ILE

73,5

3 ILE

75,4

4 L

EU

77,4

6 N

ORLEU

80,0

5 T

YR

Seite 2 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Physiological Extended +

number retention time

code


code [min]

[min]

1 3,8 SULFO

27 71,2 AILE

2 6 TAU

28 73,5 ILE

3 7,1 PEA

29 75,4 LEU

4 9,6 Urea

30 77,5 NORLE

5 13,7 GLUA

31 80 TYR

6 21,3 ASP

32 86,3 PHE

7 24,5 HYP

34 91 HCY2

8 28,2 THR

35 95,8 BALA

9 30,3 SER

36 96,8 ANHYD ASA

10 34,2 ASN

37 97,2 BAIB

11 36,5 GLU

38 99,5 ALEVA

12 39,3 GLN

39 100,1 GABA

13 39,3 SAR

40 101,2 KYN

14 45,6 AAA

41 102,7 HIS

15 48,9 PRO

42 104 1MHIS

16 50,8 GLY

43 106,2 3MHIS

17 53,7 ALA

44 108,9 TRP

18 56,3 CIT

45 112,7 CAR

19 59,5 ABU

46 113,7 HCAR

20 65,9 VAL

47 117,1 ANS

21 67,6 SAC

48 119,6 AEC

22 68 CYS2

49 120,3 HYL

23 68,6 ASA

50 121,8 ORN

24 69,2 PIP

51 123,6 LYS

25 69,7 CYSTA

52 126,6 Ammonia

26 70,2 MET

53 128,9 ETH

54 135,1 ARG

Concentration: 100 nmol amino acid / ml ((Cys) 2: 50 nmol / ml)

Seite 3 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Seite 4 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Clinical

Applications

Seite 5 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Physiological (125x3/iVD)


Column ......................... : Lithium system, 125mm BL x 3.0 mm ID



Runtime ........................ : 103 min











3.9

95

' P

-Ser

5.3

29

' T

au

6.2

55

' P

EA

7.9

23

' U

rea

18

.00

8'

Asp

20

.63

8'

Hyp

ro

24

.34

6'

Th

r

26

.46

0'

Ser

29

.74

7'

Asn 31

.45

1'

Glu

34

.46

6'

Sar

35

.95

5'

a-A

AA

37

.80

6'

Pro

39

.55

8'

Gly

40

.96

6'

Ala

42

.61

6'

Cit

43

.95

3'

a-A

BA

47

.23

9'

Val

49

.21

0'

(Cy

s)2

50

.73

3'

Cy

sth

a5

1.3

95

' M

et

52

.79

4'

Ile

53

.97

8'

Leu

55

.23

6'

Nle

56

.91

7'

Ty

r

60

.27

2'

Ph

e

66

.88

1'

H-C

yst

ine

69

.97

4'

b-A

la7

0.4

20

' b

-AiB

A

71

.81

9'

g-A

BA

74

.17

4'

His

75

.00

2'

1M

ehis

76

.36

8'

3M

ehis

78

.81

5'

Trp

80

.34

9'

Car

82

.98

1'

Ans

90

.41

6'

Hyly

s

91

.64

2'

Orn

93

.12

5' L

eu

95

.50

0'

NH

4

97

.34

6'

EO

HN

H2

10

1.5

66' A

rg

Seite 6 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Physiological (125x3/iVD)


code


code [min]

[min]

1 42616 P-Ser

22 52.8 Ile

2 42434 Tau

23 53.9 Leu

3 42406 PEA

24 55.2 Nle

4 42620 Urea

25 56.9 Tyr

5 18.0 Asp

26 60.2 Phe

6 42541 Hypro

27 66.9 H-Cystin

7 42453 Thr

28 69.9 b-Ala

8 42516 Ser

29 70.4 b-Aiba

9 42580 Asn

30 71.8 g-ABA

10 42521 Glu

31 74.1 His

11 34.5 Sar

32 75.0 1Mehis

12 35.9 a-AAA

33 76.4 3Mehis

13 37.8 Pro

34 78.8 Trp

14 39.6 Gly

35 80.3 Car

15 40.9 Ala

36 82.9 Ans

16 42.6 Cit

37 90.4 Hylys

17 43.9 a-ABA

38 91.6 Orn

18 47.2 Val

39 93.1 Lys

19 49.2 (Cys)2

40 95.5 NH4

20 50.7 Cystha

41 97.3 EOHNH2

21 51.4 Met

42 101.6 Arg


Seite 7 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Physiological (150x4)





Runtime ........................ : 128 min











4.1

28

' P

-Ser

6.1

35

' T

au7

.30

9'

PE

A

9.6

91

' U

rea

21

.02

8'

Asp

24

.00

3'

Hyp

ro

28

.17

3'

Th

r

30

.29

2'

Ser

34

.37

7'

Asn

36

.68

4'

Glu

39

.42

0' G

ln

42

.48

7'

Sar

45

.29

7'

a-A

AA

48

.44

6'

Pro

50

.24

1'

Gly

52

.86

5'

Ala

55

.06

0'

Cit

58

.05

3'

a-A

BA

63

.93

8'

Val

66

.46

8'

(Cy

s)2

67

.99

9'

Cy

sth

a6

9.3

40

' M

et

71

.45

0'

Ile

73

.14

5'

Leu

75

.01

0'

Nle

77

.46

0'

Ty

r

82

.66

2'

Ph

e

85

.74

6'

H-C

yst

ine

90

.95

0'

b-A

la

93

.85

4'

b-A

iBA

97

.10

2'

g-A

BA

10

0.4

10' H

is

10

1.7

46' 1

Meh

is

10

4.2

38' 3

Meh

is

10

5.5

99' T

rp

11

1.0

49' C

ar

11

3.9

05' A

ns

11

5.9

27' H

yly

s1

17

.18

1' O

rn

11

8.5

68' L

ys

12

1.7

72' N

H4

12

3.7

54' E

OH

NH

2

12

9.7

96' A

rg

Seite 8 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Physiological (150x4)


code


code [min]

[min]

1 4.1 P-Ser

23 71.5 Ile

2 6.1 Tau

24 73.1 Leu

3 7.3 PEA

25 73.1 Nle

4 9.7 Urea

26 75.0 Tyr

5 21.0 Asp

27 77.5 Phe

6 24.0 Hypro

28 82.7 H-Cystin

7 28.2 Thr

29 85.7 b-Ala

8 30.3 Ser

30 90.9 b-Aiba

9 34.4 Asn

31 93.9 g-ABA

10 36.7 Glu

32 97.1 His

11 39.4 Gln

34 100.4 1Mehis

12 42.5 Sar

35 101.7 3Mehis

13 45.3 a-AAA

36 104.2 Trp

14 48.4 Pro

37 105.6 Car

15 50.2 Gly

38 111.0 Ans

16 52.9 Ala

39 113.9 Hylys

17 55.1 Cit

40 115.9 Orn

18 58.1 a-ABA

41 117.2 Lys

19 63.9 Val

42 121.8 NH4

20 66.5 (Cys)2

43 123.8 EOHNH2

21 67.9 Cystha

44 129.8 Arg

22 69.3 Met


Seite 9 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Diagnostic short (MSUD)




Eluents .......................... : Lithium system - Eluent B, C and Eluent F

Runtime ........................ : 34 min









Sample .......................... : Diagnostic standard solution


17

.46

3'

Val

19

.54

6'

Met

20

.48

1'

a-Il

e

21

.60

4'

Ile

22

.68

9'

Leu

24

.48

2'

Nle

25

.92

7'

Ty

r

31

.39

3'

Ph

e

Seite 10 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Diagnostic short (MSUD)



[min] code

1 17.5 Val

2 19.5 Met

3 20.5 a-Ile

4 21.6 Ile

5 22.7 Leu

6 24.5 Nle

7 25.9 Tyr

8 31.4 Phe

Seite 11 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Seite 12 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Food and

Feedstuff

Applications

Seite 13 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Hydrolysate (125x3)





Runtime ........................ : 60 min









Sample .......................... : Hydrolysate standard solution


12

.74

7' A

sp

18

.36

9'

Th

r

19

.58

6'

Sar

20

.86

6'

Glu

24

.70

8'

Pro

26

.13

9'

Gly

27

.43

1'

Ala

28

.44

2' (

Cy

s)2

28

.94

4'

Val

30

.54

2'

Met

32

.14

1'

Ile

32

.91

3'

Leu

34

.49

1'

Ty

r

35

.73

2' P

he

39

.04

8'

His

44

.67

4'

Ly

s

47

.07

9'

NH

4

52

.15

4' A

rg

Channel A

Seite 14 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Hydrolysate (125x3)



[min] code

1 12.7 Asp

2 18.3 Thr

3 19.6 Ser

4 20.9 Glu

5 24.7 Pro

6 26.1 Gly

7 27.4 Ala

8 28.4 (Cys)2

9 28.9 Val

10 30.5 Met

11 32.1 Ile

12 32.9 Leu

13 34.5 Tyr

14 35.7 Phe

15 39.0 His

16 44.7 Lys

17 47.1 NH4

18 52.2 Arg

Seite 15 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Hydrolysate (125x4)





Runtime ........................ : 60 min









Sample .......................... : Hydrolysate standard solution


15

.43

7'

Asp

23

.11

4'

Th

r

24

.53

5'

Ser

25

.81

6'

Glu

31

.09

6'

Pro

32

.57

8'

Gly

34

.72

0'

Ala 3

5.4

17

' (C

ys)

2 36

.22

2'

Val

38

.09

7'

Met

40

.09

7'

Ile

41

.06

6'

Leu 4

2.6

68

' T

yr

44

.35

4' P

he

45

.64

2'

His

50

.56

9' L

ys

53

.92

2'

NH

4

59

.55

4'

Arg

Channel A Channel B

Seite 16 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Hydrolysate (125x4)



[min] code

1 15.4 Asp

2 23.1 Thr

3 24.5 Ser

4 25.8 Glu

5 31.1 Pro

6 32.6 Gly

7 34.7 Ala

8 35.4 (Cys)2

9 36.2 Val

10 38.1 Met

11 40.1 Ile

12 41.1 Leu

13 42.7 Tyr

14 44.4 Phe

15 45.6 His

16 50.6 Lys

17 53.9 NH4

18 59.6 Arg

Seite 17 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Hydrolysate PLUS (TRP)





Runtime ........................ : 80 min


Eluent Flow ................... : 220 µl/min and 280µl/min




Reagent Flow ............... : 120µl/min and 140 µl/min



Sample .......................... : Hydrolysate standard solution with additional:

L-Asparagine

L-Tryptophan

L-Ornithine


25

.19

2'

Asp

33

.73

4'

Th

r

36

.17

2'

Ser

40

.60

6'

Asn

41

.67

0'

Glu

47

.17

8'

Pro

48

.72

2'

Gly

50

.15

2'

Ala

50

.95

3'

(Cy

s)2

51

.71

4'

Val

54

.33

4'

Met

56

.19

8' I

le

57

.29

8'

Leu

58

.65

6'

Ty

r

60

.61

2'

Ph

e

61

.77

4'

His

64

.78

7'

Trp

65

.98

1'

Orn

68

.83

2'

Ly

s

72

.86

4'

NH

4

79

.05

5'

Arg

Channel A

Seite 18 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Hydrolysate PLUS (TRP)



[min] code

1 25.2 Asp

2 33.7 Thr

3 36.2 Ser

4 40.6 Asn

5 41.6 Glu

6 47.2 Pro

7 48.7 Gly

8 50.2 Ala

9 50.9 (Cys)2

10 51.7 Val

11 54.3 Met

12 56.2 Ile

13 57.3 Leu

14 58.7 Tyr

15 60.6 Phe

16 61.8 His

17 64.8 Trp

18 65.9 Orn

19 68.8 Lys

20 72.9 NH4

21 79.1 Arg

Seite 19 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Feedstuff


Column ......................... : Natrium system, 125 mm BL x 4.0 mm ID


Eluents .......................... : Natrium system - Eluent A to Eluent F

Runtime ........................ : 52 min









Sample .......................... : Feedstuff standard


3.8

22

' C

ya

14

.89

1'

Me

tSO

N

16

.58

2'

Asp

18

.24

6'

Th

r

19

.90

5'

Ser

23

.58

7'

Glu

24

.63

7'

Pro

27

.23

3'

Gly

28

.25

2'

Ala

30

.79

0'

(Cy

s)2

32

.01

3'

Val

33

.70

1'

Me

t

35

.85

8'

Ile

36

.80

4'

Leu

38

.85

1'

Ty

r

40

.28

2'

Ph

e

43

.49

5'

His

46

.21

4'

Ly

s

47

.55

2'

NH

4

52

.50

9'

Arg

Chann

Seite 20 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Feedstuff



[min] code

1 3.8 Cya

2 14.9 MetSON

3 16.6 Asp

4 18.2 Thr

5 19.9 Ser

6 23.6 Glu

7 24.6 Pro

8 27.2 Gly

9 28.3 Ala

10 30.8 (Cys)2

11 32.0 Val

12 33.7 Met

13 35.9 Ile

14 36.8 Leu

15 38.9 Tyr

16 40.3 Phe

17 43.5 His

18 46.2 Lys

19 47.6 NH4

20 52.5 Arg

Seite 21 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




24 Amino Acids Feedstuff-short


Column ......................... : Natrium system, 125 mm BL x 4.0 mm ID


Eluents .......................... : Natrium system - Eluent A to Eluent F

Runtime ........................ : 26.5 min



Column Temperature ... : 50 °C and 75 °C






Sample .......................... : Feedstuff standard solution with additional:

Diaminopimelic acid

γ-Amino-n-butyric Acid

L-Ornithine and

L-Tryptophan


1.9

59

' C

ya

7.8

51

' M

etS

ON

8.4

47

' A

sp

8.9

96

' T

hr

9.7

58

' S

er

11

.49

5'

Glu

11

.74

5'

Pro

13

.14

3'

Gly

13

.57

2'

Ala

14

.29

4'

(Cy

s)2 1

5.5

20

' V

al

16

.11

7'

DA

P

16

.47

4'

Met

17

.55

3'

Ile

18

.07

6'

Leu

19

.14

2'

Ty

r

20

.53

8'

Ph

e

21

.66

1'

g-A

BA

22

.84

8'

His

23

.41

2'

Orn

23

.88

6'

Ly

s

24

.41

7'

NH

4

25

.67

6'

Trp

26

.44

5'

Ph

e

Channel A

40

80

120

160

200

240

280

320

360

400

440

480

mV

3 6 9 12 15 18 21 24 27 min

Seite 22 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




24 Amino Acids Feedstuff-short



[min] code

1 1.6 Cya

2 7.9 MetSON

3 8.4 Asp

4 9.0 Thr

5 9.8 Ser

6 11.5 Glu

7 12.2 Pro

8 13.1 Gly

9 13.6 Ala

10 14.3 (Cys)2

11 15.5 Val

12 16.1 DAP

13 16.5 Met

14 17.6 Ile

15 18.1 Leu

16 19.1 Tyr

17 20.5 Phe

18 21.7 g-ABA

19 22.8 His

20 23.4 Orn

21 23.9 Lys

22 24.4 NH4

23 25.7 Trp

24 26.4 Arg

Seite 23 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Seite 24 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Biogene

amine and

others

Seite 25 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Biogenic Amine


Column ......................... : Potassium system, 118 mm BL x 2.0 mm ID

Inline Filter, 40 mm x 2.0 mm ID

Eluents .......................... : Potassium system - Eluent A to Eluent D & Eluent F

Runtime ........................ : 40 min



Column Temperature ... : 75 °C






Sample .......................... : Biogenic Amine Standard solution


8.5

88

' D

op

amin

e

10

.89

8'

His

tam

ine

15

.51

1'

Pu

tre

scin

e

19

.46

4'

Cad

ave

rin

e

21

.59

6'

Sp

erm

idin

e

27

.32

6'

Sp

erm

ine

33

.11

5'

Ag

ma

tin

e

35

.12

4'

Ty

ram

ine

Seite 26 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Biogenic Amine


[min] code

1 8.6 Dopamine

2 10.9 Histamine

3 15.5 Putrescine

4 19.5 Cadaverine

5 21.6 Spermidine

6 27.3 Spermine

7 33.1 Agmatine

8 35.1 Tyramine

Seite 27 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Glyphosate and AMPA


Column ......................... : Lithium system, 118 mm x 3.0 mm ID and


Eluents .......................... : Lithium system - Eluent A and Eluent F



Column Temperature ... : 60 °C





Detection ...................... : Visible – 570 nm

Sample .......................... : Glyphosate

AMPA (Aminomethylphosphonic acid)

Standard Solution with 100 nmol/ml

Gly

ph

osa

te

AM

PA

0

20

40

60

80

100

120

140

160

180

200

mV

0.9 1.8 2.7 3.6 4.5 5.4 6.3 7.2 8.1 9 9.9 min

Seite 28 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Glyphosate and AMPA

Standard Solutions with 100 nmol/ml, 25 nmol/ml and 5 nmol/ml GA_5

GA_2 5

GA_1 00

20

40

60

80

10 0

12 0

14 0

16 0

18 0

20 0

mV

0.9 1.8 2.7 3.6 4.5 5.4 6.3 7.2 8.1 9 9.9 min

Gly

ph

osa

te

AM

PA

Standard Solution of Amino Acids with 100 nmol/ml

P-S

er

Tau

PE

A

Ure

a

30

60

90

120

150

180

210

240

270

300

mV

0.9 1.8 2.7 3.6 4.5 5.4 6.3 7.2 8.1 9 9.9 min

Seite 29 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Stress factors plants



Ammonium Absorber, 40 mm BL x 2.0 mm ID

Eluents .......................... : Lithium system - Eluent B to Eluent F

Runtime ........................ : 23 min


Eluent Flow ................... : 450 µL/min

Column Temperature ... : 50 °C and 70 °C



Reagent Flow ............... : 225 µL/min



Sample .......................... : Physiological Standard



Ammonium Absorber, 40 mm BL x 2.0 mm ID

Seite 30 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________




Stress factors plants

Standard Solution with 100 nmol/ml (Channel A / 570nm an Channel B /440nm)


[min] amino acid code

1 11.0 L- Proline Pro

2 22.0 L-Arginine Arg

10

.98

3'

Pro

22

.02

1'

Arg

33

.99

7'

Pro

Channel A Channel B

Seite 31 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Definitions of abbreviations

amino acid code

S-Sulfocysteine SULFO

Taurine TAU

o-Phosphoethanolamine PEA

Urea Urea

D-Glucosaminic Acid GLUA

L-Aspartic Acid ASP

Hydroxy-L-Proline HYP

L- Threonine THR

L-Serine SER

L- Asparagine ASN

L-Glutamic Acid GLU

L-Glutamine GLN

L- Sarcosine SAR

L- α-Aminoadipic Acid AAA

L- Proline PRO

Glycine GLY

L-Alanine ALA

L-Citrulline CIT

L-α-Amino-n-butyric Acid ABU

L- Valine VAL

L-Saccharopine SAC

L-Cystine CYS2

Argininosuccinic acid ASA

L-Pipecolic Acid PIP

Cystathionine CYSTA

L-Methionine MET

L-Allo-Isoleucine AILE

L-Isoleucine ILE

L-Leucine LEU

Norleucine(internal standard) NORLE

L-Tyrosine TYR

L-Phenylalanine PHE

H-Cystine HCY2

ß-Alanine BALA

Seite 32 ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________



Succinic anhydride ANHYD ASA

ß-Aminoisobutyric Acid BAIB

Amino-Levulinic Acid ALEVA

γ-Amino-n-butyric Acid GABA

Kynurenine KYN

L-Histidine HIS

1-Methyl-L-Histidine 1MHIS

3-Methyl-L-Histidine 3MHIS

L-Tryptophan TRP

L-Carnosine CAR

Homocarnosine HCAR

L-Anserine ANS

S-Aminoethyl-L-cysteine AEC

Hydroxylysine HYL

L-Ornithine ORN

L-Lysine LYS

Ammonium Chloride Ammonia

Ethanolamine ETH

L-Arginine ARG

Documents

Application Notes Aracus - Gulf Bio Analytical · Application Notes Aracus membraPure GmbH Wolfgang Küntscher Str. 14 ... 24 55.2 Nle 4 42620 Urea 25 56.9 Tyr 5 18.0 Asp 26 60.2